Traditional Chinese medicine composition for treating whelks as well as preparation method and application of traditional Chinese medicine composition

Abstract

The traditional Chinese medicine composition is prepared from the following components in parts by weight: 5 to 25 parts of radix angelicae sinensis, 3 to 20 parts of rhizoma chuanxiong, 6 to 30 parts of radix salviae miltiorrhizae, 5 to 26 parts of flos carthami, 3 to 25 parts of radix paeoniae rubra, 2 to 22 parts of radix trichosanthis, 6 to 25 parts of bulbus fritillariae cirrhosae, 6 to 20 parts of frankincense, 5 to 25 parts of myrrh, 3 to 30 parts of fructus gardeniae, 3 to 26 parts of fructus forsythiae, 5 to 30 parts of flos lonicerae, 5 to 25 parts of herba violae, 5 to 30 parts of spina gleditsiae, 3 to 30 parts of caulis bambusae in taeniam, 3 to 30 parts of herba taraxaci, 5 to 25 parts of radix rehmanniae and 3 to 30 parts of radix scrophulariae. The invention provides a prescription of angelica sinensis, ligusticum wallichii, salvia miltiorrhiza, safflower, radix paeoniae rubra, radices trichosanthis, fritillaria, frankincense, myrrh, gardenia, fructus forsythiae, honeysuckle, bunge corydalis herb, spina gleditsiae, caulis bambusae in taeniam, dandelion, radix rehmanniae and radix scrophulariae, all the medicines supplement one another, the effects of clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, reducing swelling and removing stasis and cooling blood can be achieved, and the traditional Chinese medicine has a good effect on treating whelks.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine preparation, and in particular to a Chinese medicine composition for treating acne, and a preparation method and application thereof. Background Art

[0002] Acne, commonly known as pimples, is a common chronic inflammatory skin disease of the hair follicles and sebaceous glands, and is often manifested clinically as acne, papules, pustules, nodules and cysts. The prevalence of acne is high, and it often recurs. If it is not handled or treated properly, it may cause pigmentation and scarring problems, which greatly affect the patient's body and mind. Western medicine uses oral antibiotics, retinoic acid drugs, anti-androgen drugs or glucocorticoids to treat acne, combined with topical benzoic acid peroxide, salicylic acid, dapsone, etc., but it may produce adverse reactions or side effects, drug resistance and other problems. Traditional Chinese medicine has a long history in the treatment of skin diseases with its unique method of syndrome differentiation and treatment, and has obtained multi-components, multi-pathways and multi-targets to achieve the purpose of sterilization and antibacterial. Therefore, it is an ideal choice to develop drugs with good efficacy and small side effects from traditional Chinese medicine to prevent and treat acne, which can provide a reference for the development of oral preparations that can meet the needs of clinical treatment of acne. Summary of the invention

[0003] In view of this, the purpose of the present invention is to propose a Chinese medicine composition for treating acne and its preparation method and application, and to provide an oral preparation that meets the needs of clinical treatment of acne.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A traditional Chinese medicine composition for treating acne comprises the following components by weight: 5-25 parts of angelica, 3-20 parts of chuanxiong, 6-30 parts of salvia miltiorrhiza, 5-26 parts of safflower, 3-25 parts of red peony root, 2-22 parts of trichosanthes, 6-25 parts of fritillaria, 6-20 parts of frankincense, 5-25 parts of myrrh, 3-30 parts of twig stalk, 3-26 parts of forsythia, 5-30 parts of honeysuckle, 5-25 parts of physalis, 5-30 parts of soapberry, 3-30 parts of bamboo shavings, 3-30 parts of dandelion, 5-25 parts of rehmannia root, and 3-30 parts of scrophularia.

[0006] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, a plant of the Umbelliferae family. It is sweet, pungent, and warm in nature; it enters the liver, heart, and spleen meridians. It has the effects of nourishing blood and activating blood circulation, regulating menstruation and relieving pain, and moistening the intestines and relieving constipation. It is used for blood deficiency and sallow complexion, dizziness and palpitations, irregular menstruation, amenorrhea and dysmenorrhea, asthenic abdominal pain, rheumatic arthralgia, injuries from falls, carbuncles, ulcers, and constipation caused by dry intestines. Wine Angelica sinensis activates blood circulation and regulates menstruation. It is used for amenorrhea and dysmenorrhea, rheumatic arthralgia, and injuries from falls.

[0007] Chuanxiong is the dried rhizome of Ligusticum chuanxiong Hort., a plant of the Umbelliferae family. It is pungent and warm in nature, and belongs to the liver, gallbladder, and pericardium meridians. Its functions and indications are promoting blood circulation and qi movement, dispelling wind and relieving pain. It is used for chest pain due to chest impediment, stabbing pain in the chest and hypochondrium, swelling and pain from falls and bruises, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to mass in the abdomen, headache, and pain due to wind-damp arthralgia.

[0008] Danshen is the dried root and rhizome of Salvia miltiorrhiza Bge., a plant of the Labiatae family. It is bitter and slightly cold in nature, and belongs to the heart and liver meridians. Its functions and indications are promoting blood circulation to remove stasis, dredging meridians to relieve pain, clearing the heart to remove vexation, cooling blood and dissipating carbuncles. It is used for chest pain due to chest impediment, pain in the epigastrium and hypochondrium, mass accumulation, pain due to heat arthralgia, restlessness and insomnia, irregular menstruation, amenorrhea and dysmenorrhea, swelling and pain of sores and ulcers.

[0009] Honghua is the dried flower of Carthamus tinctorius L., a plant of the Compositae family. It is pungent and warm in nature, and belongs to the heart and liver meridians. Its functions and indications are promoting blood circulation to dredge menstruation, dispersing stasis and relieving pain. It is used for amenorrhea, dysmenorrhea, retention of lochia, mass in the abdomen, chest pain due to chest impediment, stasis abdominal pain, stabbing pain in the chest and hypochondrium, falls and bruises, swelling and pain of sores and ulcers.

[0010] Chishao is the dried root of Paeonia lactiflora Pall. or Paeonia veitchii Lynch, plants of the Ranunculaceae family. It is bitter and slightly cold in nature, and belongs to the liver meridian. Its functions and indications are clearing heat and cooling blood, dispersing stasis and relieving pain. It is used for heat entering nutrient blood, epidemic febrile disease with maculae, hematemesis and epistaxis, red and painful eyes, hypochondriac pain due to stagnation of liver qi, amenorrhea and dysmenorrhea, abdominal pain due to mass in the abdomen, falls and bruises, carbuncles and sores.

[0011] Tianhuafen is the dried root of Trichosanthes kirilowii Maxim. or Trichosanthes rosthornii Harms, plants of the Cucurbitaceae family. It is sweet, slightly bitter, and slightly cold in nature, and belongs to the lung and stomach meridians. Its functions and indications are clearing heat and purging fire, promoting the production of body fluid to quench thirst, dissipating swelling and discharging pus. It is used for feverish vexation and thirst, dry cough due to lung heat, internal heat and thirst, swelling and toxicity of sores and ulcers.

[0012] Fritillaria, a plant of the genus Fritillaria in the Liliaceae family, has bulbs used medicinally for relieving cough and resolving phlegm, clearing heat and dissipating nodules. Sichuan Fritillaria is bitter, sweet, and slightly cold in nature; Zhejiang Fritillaria is bitter and cold in nature, and belongs to the lung and heart meridians. Its effects are resolving phlegm and relieving cough, clearing heat and dissipating nodules. It is mainly used for cough with thick phlegm due to heat, external cough, yin deficiency cough, scanty phlegm with dry throat, thick yellow phlegm, lung abscess, breast abscess, swelling and toxicity of sores and ulcers, scrofula, etc. Modern pharmacological experiments have shown that Fritillaria has effects such as relieving cough, lowering blood pressure, and increasing blood sugar.

[0013] Frankincense is the resin exuded from the bark of Boswellia carterii Birdw. of the Burseraceae family and plants of the same genus, Boswellia bhaw-dajiana Birdw. It is pungent, bitter, and warm in nature, and belongs to the heart, liver, and spleen meridians. Its functions and indications are: activating blood circulation to relieve pain, reducing swelling and promoting granulation. It is used for chest impediment pain, epigastric pain, dysmenorrhea and amenorrhea, postpartum stasis, abdominal pain due to mass in the abdomen, rheumatic arthralgia, muscle contracture, traumatic injury, carbuncles and sores.

[0014] Myrrh is the dried resin of Commiphora myrrha Engl. or Commiphora molmol Engl. of the Burseraceae family. It is pungent, bitter, and neutral in nature, and belongs to the heart, liver, and spleen meridians. Its functions and indications are: promoting blood circulation to remove stasis and relieve pain, reducing swelling and promoting granulation. It is used for chest impediment pain, epigastric pain, dysmenorrhea and amenorrhea, postpartum stasis, abdominal pain due to mass in the abdomen, rheumatic arthralgia, traumatic injury, carbuncles and sores.

[0015] Gardenia, also known as cape jasmine fruit, is a traditional Chinese medicine name. It is the dried ripe fruit of Gardenia jasminoides Ellis of the Rubiaceae family. It is bitter and cold in nature, and belongs to the heart, lung, and triple energizer meridians. Its functions and indications are: purging fire and relieving restlessness, clearing away heat and promoting diuresis, cooling blood and detoxifying; reducing swelling and relieving pain externally. It is used for restlessness due to febrile diseases, damp-heat jaundice, stranguria with pain, hematemesis and epistaxis due to blood heat, red and swollen eyes, sores and ulcers caused by fire toxin; externally treating sprains and contusions.

[0016] Forsythia fruit is the dried fruit of Forsythia suspensa (Thunb.) Vahl of the Oleaceae family. It is bitter and slightly cold in nature, and belongs to the lung, heart, and small intestine meridians. Its functions and indications are: clearing heat and detoxifying, reducing swelling and dissipating nodules, dispersing wind-heat. It is used for carbuncles, scrofula, breast abscess, erysipelas, wind-heat cold, the initial stage of warm diseases, heat entering the nutrient system, high fever with restlessness, coma and maculae, stranguria with pain due to heat.

[0017] Honeysuckle flower is the dried flower bud or the flower with the initial opening of Lonicera japonica Thunb. of the Caprifoliaceae family. It is sweet and cold in nature, and belongs to the lung, heart, and stomach meridians. Its functions and indications are: clearing heat and detoxifying, dispersing wind-heat. It is used for carbuncles and boils, sore throat, erysipelas, dysentery with heat toxin, wind-heat cold, fever of warm diseases.

[0018] Corydalis bungeana Turcz., also known as Corydalis bungeana, is a perennial herb of the genus Corydalis in the Papaveraceae family. It is cold in nature, bitter in taste, slightly poisonous, and belongs to the heart and spleen meridians. Its functions and indications are: clearing heat and detoxifying. It is used to treat high fever and restlessness due to warm diseases, influenza, infectious hepatitis, nephritis, scrofula, mumps, boils and other suppurative infections.

[0019] Soap needle, Gleditsia sinensis Lam., also known as Chinese honey locust, honey locust needle, knife soap, etc., with the Chinese name soap thorn, is a plant of the genus Gleditsia in the legume family of the Fabales order. It is pungent in taste, warm in nature, and belongs to the liver and lung meridians. Its functions and indications are: disinfecting and promoting pus discharge, expelling wind, and killing insects. It is mainly used for carbuncles and sores, scrofula, stubborn skin diseases such as sores and tinea, lack of milk after childbirth, retention of the placenta, and leprosy. It contains flavonoid glycosides, phenols, and amino acids. The flavonoid compounds are fustin (i.e., 3,7,3,4,-tetrahydroxyflavanone) and fisetin (i.e., 3,7,3,4-tetrahydroxyflavone), and also contains colorless anthocyanins.

[0020] Bamboo shavings are the dried middle layer of the stems of Bambusa tuldoides Munro, Sinocalamus beecheyanus (Munro) McClure var. Pubescens P.F.Li, or Phyllostachys nigra (Lodd.) Munro var. henonis (Mitf.) Stapf ex Rendle, which are plants of the Poaceae family. Functions and indications: clearing heat and resolving phlegm, relieving restlessness, and stopping vomiting. It is used for cough with phlegm-heat, phlegm-fire, restlessness and uneasiness, insomnia due to restlessness, stroke with phlegm obstruction, stiffness of the tongue and inability to speak, and vomiting due to stomach heat.

[0021] Dandelion is the dried whole herb of Taraxacum mongolicum Hand.-Mazz., Taraxacum sinicum Kitag., or several other plants of the same genus in the Asteraceae family. Its nature and flavor are bitter, sweet, and cold; its meridian tropism is the liver and stomach meridians. Functions and indications: clearing heat and detoxifying, reducing swelling and dissipating nodules, and promoting diuresis and relieving stranguria. It is used for furuncles and carbuncles, breast abscess, scrofula, red eyes, sore throat, lung abscess, intestinal abscess, damp-heat jaundice, and painful and difficult urination.

[0022] Raw rehmannia usually refers to Rehmannia glutinosa Libosch., the fresh or dried tuberous root of the plant Rehmannia glutinosa Libosch. of the Scrophulariaceae family. Fresh rehmannia is sweet, bitter, and cold in nature, and belongs to the heart, liver, and kidney meridians. Raw rehmannia is sweet and cold in nature, and belongs to the heart, liver, and kidney meridians. Functions and indications: Fresh rehmannia is used for clearing heat and promoting fluid production, cooling blood, and stopping bleeding. It is used for injury to yin due to febrile diseases, crimson tongue and vexatious thirst, maculae caused by warm-toxicity, vomiting of blood, epistaxis, and sore throat. Raw rehmannia is used for clearing heat and cooling blood, nourishing yin and promoting fluid production. It is used for heat entering nutrient-blood, maculae caused by warm-toxicity, vomiting of blood and epistaxis, injury to yin due to febrile diseases, crimson tongue and vexatious thirst, constipation due to fluid impairment, yin deficiency fever, steaming bone fever, and internal heat with thirst.

[0023] Scrophulariae Radix is the dried root of the plant Scrophularia ningpoensis Hemsl. of the family Scrophulariaceae. It is sweet, bitter, salty in flavor and slightly cold in nature, and acts on the lung, stomach and kidney meridians. Its functions and indications include clearing heat and cooling blood, nourishing yin and reducing fire, detoxifying and dissipating binds. It is used for heat invading nutrient-blood, epidemic toxin maculae, febrile disease injuring yin, crimson tongue with restlessness and thirst, fluid impairment and constipation, consumptive fever with dyspnea, red eyes, sore throat, diphtheria, scrofula, carbuncles, sores and toxic swelling.

[0024] A further formulation, by weight parts, comprises the following components: 15 - 25 parts of Angelicae Sinensis Radix, 5 - 15 parts of Chuanxiong Rhizoma, 18 - 30 parts of Salviae Miltiorrhizae Radix et Rhizoma, 15 - 26 parts of Carthami Flos, 15 - 25 parts of Paeoniae Radix Rubra, 5 - 12 parts of Trichosanthis Radix, 15 - 25 parts of Fritillariae Thunbergii Bulbus, 12 - 20 parts of Olibanum, 15 - 25 parts of Myrrha, 10 - 20 parts of Gardeniae Fructus, 15 - 26 parts of Forsythiae Fructus, 10 - 20 parts of Lonicerae Japonicae Flos, 5 - 15 parts of Violae Herba, 10 - 30 parts of Gleditsiae Spina, 10 - 20 parts of Bambusae Caulis in Taeniam, 10 - 20 parts of Taraxaci Herba, 5 - 15 parts of Rehmanniae Radix, 10 - 20 parts of Scrophulariae Radix.

[0025] A still further formulation, by weight parts, comprises the following components: 15 - 18 parts of Angelicae Sinensis Radix, 12 - 15 parts of Chuanxiong Rhizoma, 18 - 20 parts of Salviae Miltiorrhizae Radix et Rhizoma, 15 - 18 parts of Carthami Flos, 15 - 18 parts of Paeoniae Radix Rubra, 10 - 12 parts of Trichosanthis Radix, 15 - 18 parts of Fritillariae Thunbergii Bulbus, 12 - 15 parts of Olibanum, 15 - 18 parts of Myrrha, 18 - 20 parts of Gardeniae Fructus, 15 - 18 parts of Forsythiae Fructus, 18 - 20 parts of Lonicerae Japonicae Flos, 12 - 15 parts of Violae Herba, 10 - 12 parts of Gleditsiae Spina, 18 - 20 parts of Bambusae Caulis in Taeniam, 18 - 20 parts of Taraxaci Herba, 12 - 15 parts of Rehmanniae Radix, 18 - 20 parts of Scrophulariae Radix.

[0026] A further preferred formulation, by weight parts, comprises the following components: 15 g of Angelicae Sinensis Radix, 12 g of Chuanxiong Rhizoma, 18 g of Salviae Miltiorrhizae Radix et Rhizoma, 15 g of Carthami Flos, 15 g of Paeoniae Radix Rubra, 12 g of Trichosanthis Radix, 15 g of Fritillariae Thunbergii Bulbus, 12 g of Olibanum, 15 g of Myrrha, 18 g of Gardeniae Fructus, 15 g of Forsythiae Fructus, 18 g of Lonicerae Japonicae Flos, 15 g of Violae Herba, 12 g of Gleditsiae Spina, 18 g of Bambusae Caulis in Taeniam, 18 g of Taraxaci Herba, 15 g of Rehmanniae Radix, 18 g of Scrophulariae Radix.

[0027] On the one hand, the present invention provides a preparation method of the above-mentioned traditional Chinese medicine composition for treating acne, which comprises the following steps:

[0028] (1) According to the above formula, take Olibanum and Myrrha, and soak them in a 70% ethanol solution by a material-liquid weight ratio of 1:3 - 5 for 2 - 3 h to obtain a soaking solution.

[0029] (2) According to the above formula, cut each medicinal material, add water 6 - 8 times the weight of the raw materials and soak for 1 - 2 h, then add the soaking solution obtained in step (1), decoct for 2 - 3 h, filter, take the filtrate, add water 5 - 7 times the weight of the filter residue to the filter residue, decoct for 2 - 3 h, filter, and combine the two filtrates to obtain a decoction solution.

[0030] A traditional Chinese medicine preparation for treating acne, comprising the above-mentioned traditional Chinese medicine composition for treating acne and pharmaceutically acceptable excipients.

[0031] A further scheme is that the traditional Chinese medicine preparation for treating acne includes one of decoction, granule, and ointment.

[0032] A still further scheme is that the preparation method of the decoction includes the following:

[0033] (1) According to the above formula, take frankincense and myrrh, and soak them in a 70% ethanol solution by volume at a material-liquid weight ratio of 1:3 - 5 for 2 - 3 h to obtain an immersion solution.

[0034] (2) According to the above formula, cut each medicinal material, add water 6 - 8 times the weight of the raw materials and soak for 1 - 2 h, then add the immersion solution obtained in step (1), decoct for 2 - 3 h, filter, take the filtrate, add water 5 - 7 times the weight of the filter residue to the filter residue, decoct for 2 - 3 h, filter, and combine the two filtrates to obtain the decoction.

[0035] On the other hand, the present invention also provides the application of the above-mentioned traditional Chinese medicine composition for treating acne in the preparation of drugs for treating acne.

[0036] A further scheme is that the application in the preparation of drugs for reducing inflammatory factors from the traditional Chinese medicine composition for treating acne, and the inflammatory factors are IL-1β and / or TNF-α.

[0037] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0038] The present invention provides a prescription of angelica, chuanxiong, salvia miltiorrhiza, safflower, red peony root, trichosanthes root, fritillaria thunbergii, frankincense, myrrh, gardenia jasminoides, forsythia suspensa, honeysuckle flower, viola yedoensis, Chinese honey locust spine, bamboo shavings, dandelion, rehmannia glutinosa, and scrophularia ningpoensis. The compatibility of this prescription is mainly for clearing heat and detoxifying, promoting blood circulation to remove stasis, and supplemented by the effects of detumescence and dissipating nodules, cooling blood. It is used to treat acne caused by qi and blood stasis and external pathogen invasion. Forsythia suspensa, honeysuckle flower, viola yedoensis, dandelion, etc. in the prescription have the effect of clearing heat and detoxifying, and can be used to treat diseases caused by heat toxin. Angelica, chuanxiong, salvia miltiorrhiza, safflower, red peony root, etc. have the effect of promoting blood circulation to remove stasis and can be used to treat diseases caused by qi and blood stasis. Trichosanthes root, fritillaria thunbergii, frankincense, myrrh, Chinese honey locust spine, etc. have the effect of detumescence and dissipating nodules. Bamboo shavings has the effects of clearing heat and resolving phlegm, removing vexation and stopping vomiting, and can be used to treat restlessness and insomnia, etc. Rehmannia glutinosa and scrophularia ningpoensis have the effects of clearing heat and cooling blood, nourishing yin and reducing fire, and can be used to treat heat entering nutrient blood, yin deficiency and excessive fire, etc. All the medicines complement each other and have a good effect in treating acne.

[0039] The traditional Chinese medicine composition for treating acne can reduce the levels of IL-1β and TNF-α in the serum of mice in the mouse acne model, indicating that the compatibility of this formula can inhibit the release of inflammatory factors and has a good therapeutic effect on the skin inflammation of acne mice induced by P.acnes. Detailed implementation manners

[0040] To better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0041] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0042] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can all be obtained from commercial channels.

[0043] Propionibacterium acnes (P.acnes) was purchased from Beijing Beina Biotechnology Co., Ltd.

[0044] After the decoction of the traditional Chinese medicine composition was concentrated, the crude drug content was about 2.6 - 2.8 g / mL.

[0045] Example 1

[0046] The formula of the traditional Chinese medicine composition for treating acne is as follows:

[0047] Angelica sinensis 15 g, Ligusticum wallichii 12 g, Salvia miltiorrhiza 18 g, Carthamus tinctorius 15 g, Paeonia lactiflora 15 g, Trichosanthes kirilowii 12 g, Fritillaria thunbergii 15 g, Olibanum 12 g, Myrrha 15 g, Gardenia jasminoides 18 g, Forsythia suspensa 15 g, Lonicera japonica 18 g, Viola yedoensis 15 g, Gleditsia sinensis 12 g, Bambusa ventricosa 18 g, Taraxacum mongolicum 18 g, Rehmannia glutinosa 15 g, Scrophularia ningpoensis 18 g.

[0048] The preparation method of the decoction of the above traditional Chinese medicine composition is as follows:

[0049] (1) According to the above formula, take olibanum and myrrha, and soak them in a 70% ethanol solution by volume at a material-liquid weight ratio of 1:3 for 2 h to obtain a soaking solution;

[0050] (2) According to the above formula, cut each medicinal material, add water 6 times the weight of the raw materials and soak for 1 h, then add the soaking solution obtained in step (1), decoct for 2 h, filter, take the filtrate, add water 5 times the weight of the filter residue to the filter residue, decoct for 2 h, filter, and combine the two filtrates to obtain a decoction.

[0051] Example 2

[0052] The formula of the traditional Chinese medicine composition for treating acne is as follows:

[0053] Angelica sinensis 25 g, Ligusticum wallichii 5 g, Salvia miltiorrhiza 30 g, Carthamus tinctorius 25 g, Paeonia lactiflora 25 g, Trichosanthes kirilowii 5 g, Fritillaria thunbergii 25 g, Olibanum 20 g, Myrrha 25 g, Gardenia jasminoides 10 g, Forsythia suspensa 25 g, Lonicera japonica 10 g, Viola yedoensis 5 g, Gleditsia sinensis 30 g, Bambusa ventricosa 10 g, Taraxacum mongolicum 10 g, Rehmannia glutinosa 5 g, Scrophularia ningpoensis 10 g.

[0054] The preparation method of the decoction of the above traditional Chinese medicine composition is the same as that of Example 1.

[0055] Example 3

[0056] The formula of the traditional Chinese medicine composition for treating acne is as follows:

[0057] Angelica sinensis 5 g, Ligusticum wallichii 20 g, Salvia miltiorrhiza 8 g, Carthamus tinctorius 5 g, Paeonia lactiflora 5 g, Trichosanthes kirilowii 25 g, Fritillaria thunbergii 8 g, Olibanum 8 g, Myrrha 5 g, Gardenia jasminoides 30 g, Forsythia suspensa 5 g, Lonicera japonica 30 g, Viola yedoensis 25 g, Gleditsia sinensis 5 g, Bambusa ventricosa 30 g, Taraxacum mongolicum 30 g, Rehmannia glutinosa 25 g, Scrophularia ningpoensis 30 g.

[0058] The preparation method of the decoction of the above traditional Chinese medicine composition is the same as that of Example 1.

[0059] Example 4

[0060] The formula of the traditional Chinese medicine composition for treating acne is as follows:

[0061] Angelica sinensis 10 g, Ligusticum wallichii 8 g, Salvia miltiorrhiza 25 g, Carthamus tinctorius 20 g, Paeonia lactiflora 20 g, Trichosanthes kirilowii 18 g, Fritillaria thunbergii 10 g, Olibanum 15 g, Myrrha 20 g, Gardenia jasminoides 18 g, Forsythia suspensa 20 g, Lonicera japonica 12 g, Viola yedoensis 20 g, Gleditsia sinensis 18 g, Bambusa ventricosa 25 g, Taraxacum mongolicum 25 g, Rehmannia glutinosa 10 g, Scrophularia ningpoensis 25 g.

[0062] The preparation method of the decoction of the above traditional Chinese medicine composition is the same as that of Example 1.

[0063] Control Example 1

[0064] The difference between the formula of the traditional Chinese medicine composition for treating acne in this control example and that of Example 1 is that Lonicera japonica is replaced by Rheum palmatum, and Rehmannia glutinosa is replaced by Sanguisorba officinalis:

[0065] Angelica sinensis 15 g, Ligusticum wallichii 12 g, Salvia miltiorrhiza 18 g, Carthamus tinctorius 15 g, Paeonia lactiflora 15 g, Trichosanthes kirilowii 12 g, Fritillaria thunbergii 15 g, Olibanum 12 g, Myrrha 15 g, Gardenia jasminoides 18 g, Forsythia suspensa 15 g, Rheum palmatum 18 g, Viola yedoensis 15 g, Gleditsia sinensis 12 g, Bambusa ventricosa 18 g, Taraxacum mongolicum 18 g, Sanguisorba officinalis 15 g, Scrophularia ningpoensis 18 g.

[0066] The preparation method of the decoction of the above traditional Chinese medicine composition is the same as that of Example 1.

[0067] Control Example 2

[0068] This comparative example is for the formula of a traditional Chinese medicine composition for treating acne. The difference from Example 1 is that the amounts of Angelica sinensis, Ligusticum wallichii, Forsythia suspensa, and Lonicera japonica are reduced, and the lacking amounts are distributed according to the ratios of other components:

[0069] Salvia miltiorrhiza 28 g, Carthamus tinctorius 23 g, Paeonia lactiflora 23 g, Trichosanthes kirilowii Maxim. 12 g, Fritillaria thunbergii Miq. 15 g, Olibanum 12 g, Myrrha 15 g, Gardenia jasminoides Ellis 30 g, Viola philippica Cav. 25 g, Gleditsia sinensis Lam. 12 g, Bambusa ventricosa McClure 18 g, Taraxacum mongolicum Hand.-Mazz. 30 g, Rehmannia glutinosa Libosch. 15 g, Scrophularia ningpoensis Hemsl. 18 g.

[0070] The preparation method of the decoction of the above traditional Chinese medicine composition is the same as that of Example 1.

[0071] Comparative Example 3

[0072] This comparative example is for the formula of a traditional Chinese medicine composition for treating acne. The difference from Example 1 is that Gleditsia sinensis Lam. and Bambusa ventricosa McClure are not used:

[0073] Angelica sinensis 15 g, Ligusticum wallichii 12 g, Salvia miltiorrhiza 18 g, Carthamus tinctorius 15 g, Paeonia lactiflora 15 g, Trichosanthes kirilowii Maxim. 12 g, Fritillaria thunbergii Miq. 15 g, Olibanum 12 g, Myrrha 15 g, Gardenia jasminoides Ellis 18 g, Forsythia suspensa 15 g, Lonicera japonica 18 g, Viola philippica Cav. 15 g, Taraxacum mongolicum Hand.-Mazz. 18 g, Rehmannia glutinosa Libosch. 15 g, Scrophularia ningpoensis Hemsl. 18 g.

[0074] The preparation method of the decoction of the above traditional Chinese medicine composition is the same as that of Example 1.

[0075] Experimental Example

[0076] Establish a mouse acne model

[0077] Select 90 8-week-old SPF-grade BALB / c female mice. After one week of adaptive feeding, they are randomly divided into groups of 10 each: normal group, model group, drug administration groups (Example 1 - 4, Comparative Example 1 - 3). The normal group is not treated, and in the other groups, oleic acid is topically applied to the right auricle of the mice for 14 days. During this period, starting from the 8th day, 20 μL of Propionibacterium acnes suspension (5×10 7 cfu / μL) is injected to establish a mouse ear acne model. The drug administration groups are gavaged at a dose of 7.20 g·kg -1 ·d -1 . The normal group and the model group are gavaged with an equal amount of normal saline for 14 consecutive days. Observe the conditions of the mice in each group every day, and sacrifice the mice 12 h after the last administration. After centrifuging the blood, collect the supernatant and detect the expression levels of IL-1β and TNF-α using an ELISA kit. Measure the ear thickness with a caliper. The test results are shown in Table 1 and Table 2 below.

[0078] Table 1 Right ear thickness of mice in each group (n = 10)

[0079] Group Right ear thickness (cm) Normal group 0.203 Model group 0.375 Example 1 0.247 Example 2 0.266 Example 3 0.273 Example 4 0.278 Comparative example 1 0.331 Comparative example 2 0.327 Comparative example 3 0.303

[0080] After the experiment, the auricles of the mice in the normal group were soft and smooth. In the model group, the auricles of the mice showed problems such as thickened epidermis, necrosis, enlarged pores, and rough auricles, accompanied by papule-like elevations or cysts. In the medicated groups, the pathological conditions of the auricles of the mice were alleviated to varying degrees. In medicated groups 1-4 (Examples 1-4), most of the cysts or papules on the auricles of the mice were absorbed, and the problems of skin swelling, dryness, and roughness were alleviated, and the auricles gradually became smooth. As can be seen from Table 1, after treatment with the decoctions of each group, the thickness of the auricles of the mice decreased, and the degree of auricle swelling was alleviated.

[0081] Table 2 Effects of the decoctions of each group on the levels of IL-1β and TNF-α in the serum of mice ( n = 10)

[0082] Group <![CDATA[IL-1β (pg·mL -1 )]]> <![CDATA[TNF-α (pg·mL -1 )]]> Normal group 98.7±10.3 93.6±11.5 Model group 214.3±13.6 163.4±6.1 Example 1 145.4±6.3 118.6±4.8 Example 2 156.7±7.8 121.5±5.2 Example 3 163.3±8.7 127.0±4.4 Example 4 157.2±8.1 123.4±4.2 Comparative example 1 183.8±10.2 148.4±5.6 Comparative example 2 193.0±11.8 150.5±4.0 Comparative example 3 178.8±9.6 138.7±5.7

[0083] As shown in Table 2, the levels of IL-1β and TNF-α in the serum of the mice in the model group were higher than those in the normal group. Compared with the model group, the decoctions of each group had varying degrees of reduction in the levels of IL-1β and TNF-α in the serum of the mice. Compared with the comparative example, the traditional Chinese medicine composition provided in the examples of the present invention had a better degree of reduction in the levels of IL-1β and TNF-α in the serum of the mice, indicating that the prescription of Angelica sinensis, Ligusticum chuanxiong, Salvia miltiorrhiza, Carthamus tinctorius, Paeonia lactiflora, Trichosanthes kirilowii, Fritillaria thunbergii, Olibanum, Myrrha, Gardenia jasminoides, Forsythia suspensa, Lonicera japonica, Viola yedoensis, Gleditsia sinensis, Bambusa ventricosa, Taraxacum mongolicum, Rehmannia glutinosa, and Scrophularia ningpoensis provided by the present invention can inhibit the release of inflammatory factors and have a good therapeutic effect on the skin inflammation of acne-induced mice by P. acnes.

[0084] Angelica sinensis: It has the effects of enriching blood and promoting blood circulation, and moistening the intestines and relieving constipation.

[0085] Ligusticum chuanxiong: It has the functions of promoting blood circulation and qi movement, and dispelling wind and relieving pain.

[0086] Salvia miltiorrhiza: It can promote blood circulation to remove blood stasis, dredge meridians and relieve pain, clear the heart and remove vexation, and cool the blood and eliminate carbuncles.

[0087] Carthamus tinctorius: It can promote blood circulation to dredge the meridians and disperse stasis and relieve pain.

[0088] Paeonia lactiflora: It has the effects of clearing heat and cooling blood, and dispersing stasis and relieving pain.

[0089] Trichosanthes kirilowii: It can clear heat and purge fire, promote the production of body fluid and quench thirst, and reduce swelling and drain pus.

[0090] Fritillaria thunbergii: It has the functions of clearing heat and moistening the lungs, resolving phlegm and relieving cough, and dissipating nodules and eliminating carbuncles.

[0091] Olibanum: It can promote blood circulation and qi movement to relieve pain, and reduce swelling and promote granulation.

[0092] Myrrha: It can disperse stasis and relieve pain, and reduce swelling and promote granulation.

[0093] Gardenia jasminoides Ellis: It has the effects of purging fire and relieving restlessness, clearing away heat and promoting diuresis, cooling blood and detoxicating. Topically, it can reduce swelling and relieve pain.

[0094] Forsythia suspensa (Thunb.) Vahl: It can clear away heat and toxic materials, disperse swelling and dissipate binds, dispel wind-heat.

[0095] Lonicera japonica Thunb.: It has the functions of clearing away heat and toxic materials, dispelling wind-heat.

[0096] Viola philippica Cav.: It can clear away heat and toxic materials, cool blood and reduce swelling.

[0097] Gleditsia sinensis Lam.: It can reduce swelling and expel toxins, discharge pus.

[0098] Bamboo shavings: It has the effects of clearing away heat and resolving phlegm, relieving restlessness, stopping vomiting.

[0099] Taraxacum mongolicum Hand.-Mazz.: It can clear away heat and toxic materials, disperse swelling and dissipate binds, promote diuresis and relieve stranguria.

[0100] Rehmannia glutinosa Libosch.: It has the effects of clearing away heat and cooling blood, nourishing yin and promoting the production of body fluid.

[0101] Scrophularia ningpoensis Hemsl.: It can clear away heat and cool blood, nourish yin and lower pathogenic fire, detoxify and disperse binds.

[0102] All the herbs supplement and assist each other, and can achieve the effects of clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, dispersing swelling and dissipating binds, cooling blood.

[0103] The above is the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art of this technology, without departing from the principle of the present invention, several improvements and modifications can still be made, and these improvements and modifications are also regarded as the protection scope of the present invention.

Claims

1. A Chinese medicine composition for treating acne, characterized in that: The invention comprises the following components by weight: 5-25 parts of angelica sinensis, 3-20 parts of chuanxiong, 6-30 parts of salvia miltiorrhiza, 5-26 parts of safflower, 3-25 parts of red peony root, 2-22 parts of trichosanthes, 6-25 parts of fritillaria, 6-20 parts of frankincense, 5-25 parts of myrrh, 3-30 parts of twigs, 3-26 parts of forsythia, 5-30 parts of honeysuckle, 5-25 parts of physalis, 5-30 parts of soapberry, 3-30 parts of bamboo shavings, 3-30 parts of dandelion, 5-25 parts of rehmannia root, and 3-30 parts of Scrophularia ningpoensis.

2. A Chinese medicine composition for treating acne according to claim 1, characterized in that: The invention comprises the following components by weight: 15-25 parts of angelica sinensis, 5-15 parts of chuanxiong, 18-30 parts of salvia miltiorrhiza, 15-26 parts of safflower, 15-25 parts of red peony root, 5-12 parts of trichosanthes, 15-25 parts of fritillaria, 12-20 parts of frankincense, 15-25 parts of myrrh, 10-20 parts of twig nut, 15-26 parts of forsythia, 10-20 parts of honeysuckle, 5-15 parts of physalis, 10-30 parts of soapberry, 10-20 parts of bamboo shavings, 10-20 parts of dandelion, 5-15 parts of rehmannia root, and 10-20 parts of Scrophularia ningpoensis.

3. A Chinese medicine composition for treating acne as claimed in claim 2, characterized in that: The invention comprises the following components by weight: 15-18 parts of angelica sinensis, 12-15 parts of chuanxiong, 18-20 parts of salvia miltiorrhiza, 15-18 parts of safflower, 15-18 parts of red peony root, 10-12 parts of trichosanthes, 15-18 parts of fritillaria, 12-15 parts of frankincense, 15-18 parts of myrrh, 18-20 parts of twigs, 15-18 parts of forsythia, 18-20 parts of honeysuckle, 12-15 parts of physalis, 10-12 parts of soapberry, 18-20 parts of bamboo shavings, 18-20 parts of dandelion, 12-15 parts of rehmannia root, and 18-20 parts of scrophularia.

4. A Chinese medicine composition for treating acne as claimed in claim 3, characterized in that: The invention comprises the following components by weight: 15g of angelica sinensis, 12g of chuanxiong rhizome, 18g of salvia miltiorrhiza, 15g of safflower, 15g of red peony root, 12g of trichosanthes root, 15g of fritillaria, 12g of fritillaria, 15g of frankincense, 15g of myrrh, 18g of twig fruit, 15g of forsythia, 18g of honeysuckle, 15g of physalis, 12g of soapberry, 18g of bamboo shavings, 18g of dandelion, 15g of rehmannia root, and 18g of scrophularia.

5. The method for preparing the Chinese medicinal composition for treating acne according to any one of claims 1 to 4, characterized in that: These include: (1) According to the above formula, frankincense and myrrh are taken, and the weight ratio of the material to the liquid is 1:3-5, and a 70% volume concentration ethanol solution is added and soaked for 2-3 hours to obtain a soaking liquid; (2) According to the above formula, each medicinal material is chopped, and water of 6 to 8 times the weight of the raw material is added to soak for 1 to 2 hours, and then the soaking liquid obtained in step (1) is added, and decocted for 2 to 3 hours. The mixture is filtered, and the filtrate is taken. Water of 5 to 7 times the weight of the raw material is added to the residue, and the mixture is decocted for 2 to 3 hours. The mixture is filtered, and the two filtrates are combined to obtain a decoction liquid.

6. A Chinese medicine preparation for treating acne, characterized in that: The invention comprises the Chinese medicinal composition for treating acne according to any one of claims 1 to 4, and pharmaceutically acceptable excipients.

7. A Chinese medicinal preparation for treating acne according to claim 6, characterized in that: The Chinese medicine preparation for treating acne comprises one of a decoction, a granule and an ointment.

8. A Chinese medicinal preparation for treating acne according to claim 7, characterized in that: The preparation method of the decoction comprises the following steps: (1) According to the above formula, frankincense and myrrh are taken, and the weight ratio of the material to the liquid is 1:3-5, and a 70% volume concentration ethanol solution is added and soaked for 2-3 hours to obtain a soaking liquid; (2) According to the above formula, each medicinal material is chopped, and 6 to 8 times the weight of water of the raw material is added to soak for 1 to 2 hours, and then the soaking liquid obtained in step (1) is added, and decocted for 2 to 3 hours. The mixture is filtered, and the filtrate is taken. 5 to 7 times the weight of water is added to the residue, and the mixture is decocted for 2 to 3 hours. The mixture is filtered, and the two filtrates are combined to obtain a decoction.

9. Use of the Chinese medicinal composition for treating acne according to any one of claims 1 to 4 in the preparation of a drug for treating acne.

10. The use according to claim 9, characterized in that: The use of the traditional Chinese medicine composition for treating acne in preparing a drug for reducing inflammatory factors, wherein the inflammatory factors are IL-1β and / or TNF-α.