Repair type composition, repair type product and preparation method of repair type product

Abstract

The invention provides a repair type composition, a repair type product and a preparation method of the repair type product. The repair type composition comprises a composite dendrobium extract, a radix sophorae flavescentis extract and a rhizoma bletillae root extract, the number-average molecular weight of the composite dendrobium nobile extract is 6000 Da to 8000 Da, and the average viscosity of the composite dendrobium nobile extract at 25 DEG C is 100 mpa.s to 200 mpa.s; extraction sources of the composite dendrobium extract comprise dendrobium officinale, dendrobium nobile and dendrobium fimbriatum. The composite dendrobium extract is used together with the radix sophorae flavescentis extract and the rhizoma bletillae extract, so that the synergistic antibacterial and soothing effects can be achieved. Meanwhile, the number-average molecular weight of the composite dendrobium extract is small, the viscosity is low, the absorption effect of the skin on the dendrobium extract is facilitated, the repairing capacity of the dendrobium extract is improved, and the dendrobium extract is more suitable for people with sensitive skin to use.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and particularly relates to a repair composition, a repair product and a preparation method of the repair product. Background Art

[0002] Inflammation is a defense response of the body to stimuli, manifested as redness, swelling, heat, pain and dysfunction. Inflammation is the body's self-defense mechanism, but at the same time it can also cause a series of skin problems. Persistent inflammation can induce sensitive skin, damage the skin barrier, and repeatedly activate the nerve response; while sensitive skin is more vulnerable to irritation and damage, and the two produce a vicious cycle, ultimately leading to more sensitive skin. Therefore, the formation of sensitive skin is closely related to inflammation. Inflammation is one of the main factors of skin sensitivity, and skin sensitivity can promote the generation and development of inflammation.

[0003] At present, cosmetics with plant concepts on the market are widely favored by consumers. In particular, Dendrobium is widely used in cosmetics. However, most cosmetics using Dendrobium have problems of poor absorption and low antibacterial ability, and cannot be well applied to inflammatory sensitive skin. Summary of the Invention

[0004] Based on this, the present application provides a repair composition, a repair product and a preparation method of the repair product that are easily absorbed and have remarkable antibacterial ability, so as to be applicable to the repair of inflammatory sensitive skin.

[0005] The first aspect of the present application provides a repair composition, including a compound Dendrobium extract, a Sophora flavescens root extract and a Bletilla striata root extract; the number average molecular weight of the compound Dendrobium extract is 6000Da - 8000Da, and the average viscosity at 25°C is 100mpa·s - 200mpa·s; the extraction sources of the compound Dendrobium extract include Dendrobium officinale, Dendrobium nobile and Dendrobium fimbriatum.

[0006] In some embodiments, the mass ratio of the compound Dendrobium extract, the Sophora flavescens root extract and the Bletilla striata root extract is (1 - 5):(1 - 5):(1 - 5).

[0007] In some embodiments, the mass ratio of Dendrobium officinale, Dendrobium nobile and Dendrobium fimbriatum in the extraction sources of the compound Dendrobium extract is (1 - 2):(1 - 2):(1 - 2).

[0008] In some embodiments, the extraction sources of the compound Dendrobium extract further include one or more of Dendrobium huoshanense, Dendrobium moniliforme, Dendrobium phalaenopsis, Dendrobium chrysanthum and Dendrobium loddigesii.

[0009] The second aspect of the present application provides a repair product, comprising a repair composition and a collagen component. The repair composition includes a compound dendrobium extract, a sophora flavescens root extract, and a bletilla striata root extract; the number-average molecular weight of the compound dendrobium extract is 6000 Da - 8000 Da, and the average viscosity at 25 °C is 100 mPa·s - 200 mPa·s; the extraction sources of the compound dendrobium extract include dendrobium officinale, dendrobium nobile, and dendrobium fimbriatum.

[0010] In some embodiments, the mass ratio of the compound dendrobium extract, the sophora flavescens root extract, and the bletilla striata root extract is (1 - 5):(1 - 5):(1 - 5).

[0011] In some embodiments, the mass ratio of dendrobium officinale, dendrobium nobile, and dendrobium fimbriatum in the extraction sources of the compound dendrobium extract is (1 - 2):(1 - 2):(1 - 2).

[0012] In some embodiments, the extraction sources of the compound dendrobium extract further include one or more of dendrobium huoshanense, dendrobium moniliforme, dendrobium phalaenopsis, dendrobium chrysanthum, and dendrobium loddigesii.

[0013] In some embodiments, the collagen component includes one or more of soluble collagen, soluble collagen cross-linked polymer, collagen, collagen, and small molecule peptides.

[0014] In some embodiments, by mass, the repair product includes 0.1 parts - 15 parts of the compound dendrobium extract, 0.1 parts - 5 parts of the sophora flavescens root extract, 0.1 parts - 5 parts of the bletilla striata root extract, and 0.1 parts - 5 parts of the collagen component.

[0015] In some embodiments, by mass, the repair product includes 3 parts - 15 parts of the compound dendrobium extract, 1 part - 5 parts of the sophora flavescens root extract, 1 part - 5 parts of the bletilla striata root extract, and 1 part - 5 parts of the collagen component.

[0016] In some embodiments, the repair product further includes a skin conditioner, a moisturizer, a thickener, an antioxidant, a pH regulator, and water.

[0017] In some embodiments, the mass of the skin conditioner is 0.05 parts - 10 parts.

[0018] In some embodiments, the mass of the moisturizer is 0.5 parts - 20 parts.

[0019] In some embodiments, the mass of the thickener is 0.05 parts - 3 parts.

[0020] In some embodiments, the mass of the antioxidant is 0.05 parts - 0.3 parts.

[0021] In some embodiments, the mass fraction of the pH regulator is 0.05 parts - 3 parts.

[0022] In some embodiments, the humectant includes one or more of polyhydric alcohols such as glycerol, glucosylglycerol, 1,3 - propanediol, 1,2 - propanediol, butanediol, 1,2 - hexanediol, 1,2 - pentanediol, dipropylene glycol, sorbitol, sodium hyaluronate, hydrolyzed sodium hyaluronate, sodium polyglutamate, trehalose, erythritol, β - glucan, and betaine.

[0023] In some embodiments, the thickener includes one or more of carbomer, acrylate / C10 - 30 alkyl acrylate cross - linked polymer, xanthan gum, Chondrus crispus, sodium alginate, hydroxyethyl cellulose, acryloyldimethyltaurine ammonium / VP copolymer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, polyacrylate cross - linked polymer - 6, and hydrolyzed scleroglucan.

[0024] In some embodiments, the antioxidant includes one or more of vitamin E, tocopheryl acetate, tocotrienol, p - hydroxyacetophenone, and p - hydroxypropiophenone.

[0025] In some embodiments, the pH regulator includes one or more of triethanolamine, arginine, sodium hydroxide, and aminomethylpropanol.

[0026] In some embodiments, the skin conditioner includes one or more of ceramides, phytosterols, fermentation filtrates, and plant extracts.

[0027] In some embodiments, the plant extract includes one or more of asiatic pennywort extract, silybum marianum seed oil, gastrodia elata root extract, salvia miltiorrhiza root extract, thymus vulgaris leaf extract, coptis chinensis root extract, dendrobium chrysotoxum extract, dendrobium phalaenopsis flower extract, and dendrobium loddigesii extract.

[0028] In some embodiments, the type of the repair product includes essence.

[0029] The third aspect of the present application provides a preparation method of a repair product, including the following steps:

[0030] Perform water extraction on sophora flavescens root powder, bletilla striata root powder, and a composite dendrobium composition powder containing dendrobium officinale, dendrobium nobile, and dendrobium fimbriatum respectively to prepare sophora flavescens root extract, bletilla striata root extract, and a primary composite dendrobium extract;

[0031] Mix the primary composite dendrobium extract with lactobacillus plantarum and then ferment;

[0032] The product obtained after fermentation is subjected to enzyme removal treatment to prepare a compound Dendrobium extract;

[0033] A mixed solution containing the Sophora flavescens root extract, the Bletilla striata root extract, the compound Dendrobium extract and a collagen component is prepared to obtain the repair product;

[0034] Among them, the mass ratio of the compound Dendrobium crude extract to the Lactobacillus plantarum is (200-600):1; the number-average molecular weight of the compound Dendrobium extract is 6000Da-8000Da, and the average viscosity at 25°C is 100mpa·s-200mpa·s.

[0035] In some embodiments, the preparation method includes at least one of the following conditions:

[0036] (1) The temperature of the fermentation is 26°C-28°C, and the time is 3 days-5 days;

[0037] (2) When preparing the compound Dendrobium crude extract, the single water extraction time is 1.5h-2h, the number of water extraction times is 1-4 times, the water extraction temperature is 95°C-105°C, and the material-water mass ratio is 1:(20-40);

[0038] (3) When preparing the Sophora flavescens root extract, the single water extraction time is 1.5h-2h, the number of water extraction times is 1-4 times, the water extraction temperature is 95°C-105°C, and the material-water mass ratio is 1:(20-40);

[0039] (4) When preparing the Bletilla striata root extract, the single water extraction time is 1.5h-2h, the number of water extraction times is 1-4 times, the water extraction temperature is 95°C-105°C, and the material-water mass ratio is 1:(20-40);

[0040] (5) The step of subjecting the product obtained after fermentation to enzyme removal treatment includes: sequentially performing ethanol enzyme removal and ethanol removal treatment on the product obtained after fermentation;

[0041] Optionally, the ethanol removal treatment is carried out by means of reduced pressure rotary evaporation;

[0042] Optionally, the process parameters of the reduced pressure rotary evaporation include: the temperature is 70°C-80°C, the rotation speed is 20rpm-40rpm, and the vacuum degree after decompression is 0.05Pa-0.095Pa;

[0043] (6) The mass-volume concentration of the Sophora flavescens root extract, the Bletilla striata root extract and the compound Dendrobium extract is independently 25mg / mL;

[0044] (7) The collagen component is a collagen complex, and the collagen complex includes a soluble collagen cross-linked polymer, a filtrate of a galactomyces fermented product and water;

[0045] Optionally, the mass ratio of the soluble collagen cross-linked polymer in the collagen complex is 4%-6%; the mass ratio of the filtrate of the galactomyces ferment filtrate in the collagen complex is 1%-5%.

[0046] For the above-mentioned repair composition, a composite dendrobium extract containing three dendrobium extracts is used in combination with sophora flavescens root extract and bletilla striata root extract. The combined antibacterial effect is better than that of a single agent, and the inhibition rate of hyaluronidase is significantly increased. Therefore, the combined use of the composite dendrobium extract with sophora flavescens root extract and bletilla striata root extract can achieve a synergistic antibacterial and soothing effect. At the same time, the composite dendrobium extract has a small number-average molecular weight and low viscosity, which is beneficial to the absorption of the dendrobium extract by the skin, improving its repair ability, and making it more suitable for use by people with sensitive skin. In addition, by combining the composite dendrobium extract with sophora flavescens root extract and bletilla striata root extract, the addition of traditional and non-traditional preservatives can be greatly reduced, making the product using this repair composition milder and more suitable for use by people with sensitive skin. Description of the Drawings

[0047] In order to more clearly illustrate the technical solutions in the embodiments of the present application and to more fully understand the present application and its beneficial effects, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can obtain other drawings based on these drawings without creative efforts.

[0048] Figure 1 The number-average molecular weight of the composite dendrobium extract in Examples 1-7 and Comparative Examples 5-7.

[0049] Figure 2 The average viscosity of the composite dendrobium extract in Examples 1-7 and Comparative Examples 5-7.

[0050] Figure 3 The diameter of the inhibition zone of the repair essence on Escherichia coli and Staphylococcus aureus in Example 1, Examples 8-13 and Comparative Examples 1-3.

[0051] Figure 4 The inhibition rate of the repair essence on hyaluronidase in Example 1, Examples 8-13 and Comparative Examples 1-4. Detailed Description of the Embodiments

[0052] To facilitate the understanding of the present invention, the present application will be described more comprehensively below with reference to relevant embodiments. The following gives the preferred embodiments of the present application. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present application more thorough and comprehensive.

[0053] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs. The terms used in the description of the present application in this specification are only for the purpose of describing specific embodiments and are not intended to limit the present application.

[0054] As used herein, the alternative ranges of the terms "and / or", "or / and", and "and / or" include any one of two or more related listed items, as well as any and all combinations of the related listed items. The said any and all combinations include any two related listed items, any more related listed items, or the combination of all related listed items. It should be noted that when at least two conjunctions selected from "and / or", "or / and", and "and / or" are used to connect at least three items, it should be understood that in this application, this technical solution undoubtedly includes the technical solution connected by "logical AND", and undoubtedly includes the technical solution connected by "logical OR".

[0055] In this application, among the technical features described in an open-ended manner, it includes a closed technical solution composed of the listed features, as well as an open technical solution including the listed features.

[0056] In this application, regarding the numerical range, unless otherwise specified, the above numerical range is considered continuous and includes the minimum value and the maximum value of this range, as well as each value between such minimum value and maximum value. Further, when the range refers to an integer, it includes each integer between the minimum value and the maximum value of this range. In addition, when multiple ranges are provided to describe features or characteristics, these ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0057] This article only specifically discloses some numerical ranges. However, any lower limit can be combined with any upper limit to form a range not explicitly recorded; and any lower limit can be combined with other lower limits to form a range not explicitly recorded. Similarly, any upper limit can be combined with any other upper limit to form a range not explicitly recorded. In addition, each separately disclosed point or single numerical value itself can be used as a lower limit or an upper limit to be combined with any other point or single numerical value or with other lower limits or upper limits to form a range not explicitly recorded.

[0058] The temperature parameters in this application, unless otherwise specified, allow both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the accuracy range controlled by the instrument. Fluctuations within ranges such as ±5°C, ±4°C, ±3°C, ±2°C, ±1°C are allowed.

[0059] In this application, unless otherwise specified, when it comes to size, particle size, and diameter, it generally refers to the average value. In this application, "particle size" and "diameter" have the same definition and both represent the average diameter of spheres or sphere-like particles.

[0060] In this text, the "suitable combination method", "suitable method", "any suitable method", etc., the "suitable" mentioned therein is subject to being able to implement the technical solution of this application, solve the technical problems of this application, and achieve the expected technical effects of this application.

[0061] In this application, "further", "even further", "especially", etc. are used for descriptive purposes and indicate differences in content, but should not be understood as limiting the protection scope of this application.

[0062] In this application, "optionally", "optional", "option", mean that it can be either present or absent, that is, it refers to any one of the two parallel options of "present" or "absent". If "optional" appears multiple times in a technical solution, unless otherwise specified and there are no contradictions or mutual restrictions, each "optional" is independent.

[0063] In the description of the application, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0064] If there is no special instruction, all implementation manners and optional implementation manners of this application can be combined with each other to form a new technical solution. If there is no special instruction, all technical features and optional technical features of this application can be combined with each other to form a new technical solution.

[0065] If there is no special instruction, all steps of this application can be carried out in sequence or randomly, and preferably in sequence.

[0066] Inflammation is mainly a complex reaction caused by the interaction of various immune cells in the skin under a series of complex mechanisms. The skin cells participating in the immune inflammatory reaction regulate each other through cytokines and transmit "communication" information, ultimately leading to skin inflammation. Therefore, cosmetics for inflammatory sensitive skin have also become an important research direction.

[0067] At present, cosmetics with plant concepts are widely favored by consumers on the market, especially dendrobium, which is widely used in cosmetics. However, most cosmetics using dendrobium have many problems: First, dendrobium is used in cosmetics, mainly using its core ingredient, dendrobium polysaccharide, but the molecular weight of dendrobium polysaccharide is relatively large, and it is difficult for the skin to absorb it, and it is difficult to play its repair effect; in order to promote the skin's absorption of dendrobium, it is usually necessary to use it with a penetration enhancer, which makes it unsuitable for sensitive skin. Secondly, the sesquiterpenoid compounds contained in dendrobium have antibacterial ability, but the antibacterial ability of a single dendrobium species component is effective, and the use of preservatives cannot be greatly reduced, and its antibacterial ability to replace part of the preservatives cannot be effectively exerted. Thirdly, in the process of extraction of plant extracts, organic solvents such as methanol and ether are often selected; how to avoid the use of irritating and sensitizing solvents to ensure that the extracts do not cause new irritation and damage to the skin while exerting their efficacy is a difficulty in technical development.

[0068] Based on the above problems, the present application adopts a composite dendrobium extract containing three kinds of dendrobium extracts, which is used together with Sophora flavescens root extract and Bletilla striata root extract to obtain a repairing composition, which can play a synergistic antibacterial and soothing role. At the same time, the number average molecular weight and average viscosity of the composite dendrobium extract are regulated to promote the skin's absorption of the dendrobium extract and enhance its repair ability, so that it is suitable for people with sensitive skin.

[0069] One or more embodiments of the present application provide a repairing composition, comprising a composite dendrobium extract, a sophora flavescens root extract and a bletilla striata root extract; the number average molecular weight of the composite dendrobium extract is 6000Da-8000Da, and the average viscosity at 25°C is 100mpa·s-200mpa·s; the extraction sources of the composite dendrobium extract include Dendrobium officinale, Dendrobium nobile and Dendrobium tassellensis.

[0070] As a non-limiting example, the number average molecular weight of the composite dendrobium extract can be, but is not limited to, 6000Da, 6200Da, 6400Da, 6600Da, 6800Da, 7000Da, 7200Da, 7400Da, 7600Da, 7800Da, 8000Da or a range between any two of the above molecular weights, etc. When the number average molecular weight of the composite dendrobium extract is within the above range, it is beneficial for the composite dendrobium extract to be better absorbed and exert its repairing effect, and at the same time, it can maintain a better and more lasting moisturizing effect on the skin.

[0071] The average viscosity of the compound dendrobium extract can be, but is not limited to, 100 mPa·s, 105 mPa·s, 110 mPa·s, 115 mPa·s, 120 mPa·s, 125 mPa·s, 130 mPa·s, 135 mPa·s, 140 mPa·s, 145 mPa·s, 150 mPa·s, 155 mPa·s, 160 mPa·s, 165 mPa·s, 170 mPa·s, 175 mPa·s, 180 mPa·s, 185 mPa·s, 190 mPa·s, 195 mPa·s, 200 mPa·s, or the range between any two of the above viscosities, etc. When the average viscosity of the compound dendrobium extract is within the above range, it has a better skin feel, is not sticky, can be better absorbed by the skin and has a moisturizing effect.

[0072] It should be noted that when the mass concentration of the compound dendrobium extract is 25 mg / mL, its average viscosity is tested to be 100 mPa·s - 200 mPa·s.

[0073] The dendrobium extract mainly contains components such as polysaccharides, alkaloids, flavonoids and phenols, and sesquiterpenoids. Dendrobium polysaccharides can stimulate the function of macrophages, increase immunity and regulate biological responses, and have a good repair effect on the skin; alkaloids, flavonoids and phenols have antibacterial and anti-inflammatory effects; sesquiterpenoid compounds can effectively enhance the immune activity of the body and inhibit the growth of microorganisms such as Staphylococcus aureus, Escherichia coli, and Candida albicans. The extract of Dendrobium officinale has strong repair ability, and visa skin detection shows that the A value in the red area is significantly reduced; it shows that this dendrobium essence not only has a repair effect, but also has an anti-inflammatory and redness-reducing effect.

[0074] The Root extract of Sophora flavescens contains various alkaloids with medicinal effects. The main components are sophocarpine, D-matrine, D-oxymatrine, and also contain a small amount of D-sophoranole and L-anagyrine. The Root extract of Sophora flavescens has extremely strong anti-inflammatory and anti-allergic effects, can reduce the release of allergic mediators, is an excellent immunosuppressant, and has a good relieving effect on skin itching symptoms. The Root extract of Sophora flavescens has antibacterial effects, and has significant inhibitory effects on Gram-positive bacilli, such as Bacillus dysenteriae, Staphylococcus aureus, Salmonella albicans, and Bacillus subtilis; for Gram-negative bacteria, especially Pseudomonas aeruginosa, it has a good inhibitory effect. The Root extract of Sophora flavescens has an inhibitory effect on fungi, for example, it has different degrees of inhibitory effects on more than a dozen skin fungi such as Trichophyton rubrum.

[0075] The Bletilla striata root extract contains bibenzyl compounds and phenanthrene components. The Bletilla striata root extract has an obvious inhibitory effect on the activities of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, and has a better effect on Gram-negative bacteria than on Gram-positive bacteria, and the antibacterial effect increases with the increase of concentration.

[0076] Understandably, in the repair composition provided by the present application, a composite dendrobium extract containing three dendrobium extracts is used in combination with Sophora flavescens root extract and Bletilla striata root extract. The combined antibacterial effect is better than the single antibacterial effect, and the inhibition rate of hyaluronidase is significantly improved. Therefore, the combined use of the composite dendrobium extract with Sophora flavescens root extract and Bletilla striata root extract can play a synergistic antibacterial and soothing role. At the same time, the composite dendrobium extract has a small number-average molecular weight and low viscosity, which is beneficial to the absorption of the dendrobium extract by the skin, improves its repair ability, and makes it more suitable for use by people with sensitive skin. In addition, by combining the composite dendrobium extract with Sophora flavescens root extract and Bletilla striata root extract, the addition of traditional and non-traditional preservatives can be greatly reduced, making the product using this repair composition milder and more suitable for use by people with sensitive skin.

[0077] As a non-limiting example, the extraction sources of the composite dendrobium extract include the stems and their medicinal parts of Dendrobium officinale, the stems and their medicinal parts of Dendrobium nobile, and the stems and their medicinal parts of Dendrobium fimbriatum.

[0078] In some embodiments, the mass ratio of the compound Dendrobium extract, Sophora flavescens root extract, and Bletilla striata root extract is (1 - 5):(1 - 5):(1 - 5). As a non-limiting example, the mass ratio of the compound Dendrobium extract, Sophora flavescens root extract, and Bletilla striata root extract can be, but is not limited to, (1 - 5):(1 - 5):(1 - 5), (1 - 4.5):(1 - 4.5):(1 - 4.5), (1 - 4):(1 - 4):(1 - 4), (1 - 3.5):(1 - 3.5):(1 - 3.5), (1 - 3):(1 - 3):(1 - 3), (1 - 2.5):(1 - 2.5):(1 - 2.5), (1 - 2):(1 - 2):(1 - 2), (1 - 1.5):(1 - 1.5):(1 - 1.5), (1.5 - 5):(1.5 - 5):(1.5 - 5), (2 - 5):(2 - 5):(2 - 5), (2.5 - 5):(2.5 - 5):(2.5 - 5), (3 - 5):(3 - 5):(3 - 5), (3.5 - 5):(3.5 - 5):(3.5 - 5), (4 - 5):(4 - 5):(4 - 5), or (4.5 - 5):(4.5 - 5):(4.5 - 5), etc. When the mass ratio of the compound Dendrobium extract, Sophora flavescens root extract, and Bletilla striata root extract is within the above range, the antibacterial effect is better than that of single antibacterial agents, and the inhibition rate of hyaluronidase is significantly increased, which can play a synergistic antibacterial and soothing role; at the same time, since the three are used in combination, they have a significant antibacterial effect, and can effectively reduce the addition of traditional and non-traditional preservatives.

[0079] In some embodiments, the mass ratio of Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum in the extraction source of the compound Dendrobium extract is (1 - 2):(1 - 2):(1 - 2); for example, it can be, but is not limited to, (1.2 - 2):(1.2 - 2):(1.2 - 2), (1.4 - 2):(1.4 - 2):(1.4 - 2), (1.6 - 2):(1.6 - 2):(1.6 - 2), (1.8 - 2):(1.8 - 2):(1.8 - 2), (1 - 1.8):(1 - 1.8):(1 - 1.8), (1 - 1.6):(1 - 1.6):(1 - 1.6), (1 - 1.4):(1 - 1.4):(1 - 1.4), or (1 - 1.2):(1 - 1.2):(1 - 1.2), etc. When the mass ratio of Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum is within the above range, after being compounded with Sophora flavescens root extract and Bletilla striata root extract, the inhibition rate of the composition on hyaluronidase can reach more than 90%, and it has a good soothing effect; in addition, the diameters of the antibacterial zones of the composition against Escherichia coli (mm) and Staphylococcus aureus (mm) are both ≥20 mm, and it has a good antibacterial effect.

[0080] As a possible implementation, the extraction sources of the compound Dendrobium extract also include one or more of Dendrobium huoshanense, Dendrobium moniliforme, Dendrobium phalaenopsis, Dendrobium chrysanthum, and Dendrobium loddigesii.

[0081] One or more embodiments of the present application provide a repair product, including a repair composition and a collagen component. The repair composition includes a compound Dendrobium extract, Sophora flavescens root extract, and Bletilla striata root extract; the number-average molecular weight of the compound Dendrobium extract is 6000 Da - 8000 Da, and the average viscosity at 25°C is 100 mPa·s - 200 mPa·s; the extraction sources of the compound Dendrobium extract include Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum.

[0082] In the repair product, a compound Dendrobium extract containing three Dendrobium extracts is used in combination with Sophora flavescens root extract and Bletilla striata root extract, and at the same time, a collagen component is compounded to enhance the repair effect. It can not only ensure the repair effect of the product, but also effectively prevent microorganisms, reduce the dosage of preservatives, greatly improve the repair effect, and further enhance the soothing effect, and is suitable for use by people with inflammatory skin, sensitive skin, and acne skin caused by inflammation.

[0083] As a non-limiting example, the extraction sources of the compound Dendrobium extract include the stems and their medicinal parts of Dendrobium officinale, the stems and their medicinal parts of Dendrobium nobile, and the stems and their medicinal parts of Dendrobium fimbriatum.

[0084] The collagen component is the main component in animal connective tissues, skin, tendons, tendons, and bones. Adding a collagen component to cosmetics can partially penetrate into the skin and be absorbed by the skin to compensate for the loss of soluble collagen caused by age and light.

[0085] In some embodiments, the mass ratio of the compound Dendrobium extract, Sophora flavescens root extract, and Bletilla striata root extract is (1 - 5):(1 - 5):(1 - 5); as a non-limiting example, the mass ratio of the compound Dendrobium extract, Sophora flavescens root extract, and Bletilla striata root extract can be but is not limited to (1 - 5):(1 - 5):(1 - 5), (1 - 4.5):(1 - 4.5):(1 - 4.5), (1 - 4):(1 - 4):(1 - 4), (1 - 3.5):(1 - 3.5):(1 - 3.5), (1 - 3):(1 - 3):(1 - 3), (1 - 2.5):(1 - 2.5):(1 - 2.5), (1 - 2):(1 - 2):(1 - 2), (1 - 1.5):(1 - 1.5):(1 - 1.5), (1.5 - 5):(1.5 - 5):(1.5 - 5), (2 - 5):(2 - 5):(2 - 5), (2.5 - 5):(2.5 - 5):(2.5 - 5), (3 - 5):(3 - 5):(3 - 5), (3.5 - 5):(3.5 - 5):(3.5 - 5), (4 - 5):(4 - 5):(4 - 5), or (4.5 - 5):(4.5 - 5):(4.5 - 5), etc. When the mass ratio of the compound Dendrobium extract, Sophora flavescens root extract, and Bletilla striata root extract is within the above range, the antibacterial effect is better than that of single-component antibacterial agents, and the inhibition rate of hyaluronidase is significantly increased, which can play a synergistic antibacterial and soothing role; at the same time, since the three are used in combination with a significant antibacterial effect, the addition of traditional and non-traditional preservatives can be effectively reduced.

[0086] In some embodiments, the mass ratio of Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum in the extraction source of the compound Dendrobium extract is (1 - 2):(1 - 2):(1 - 2); for example, it can be but is not limited to (1.2 - 2):(1.2 - 2):(1.2 - 2), (1.4 - 2):(1.4 - 2):(1.4 - 2), (1.6 - 2):(1.6 - 2):(1.6 - 2), (1.8 - 2):(1.8 - 2):(1.8 - 2), (1 - 1.8):(1 - 1.8):(1 - 1.8), (1 - 1.6):(1 - 1.6):(1 - 1.6), (1 - 1.4):(1 - 1.4):(1 - 1.4), or (1 - 1.2):(1 - 1.2):(1 - 1.2), etc. When the mass ratio of Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum is within the above range, after being compounded with Sophora flavescens root extract and Bletilla striata root extract, the inhibition rate of the composition on hyaluronidase can reach more than 90%, having a good soothing effect; in addition, the diameter of the antibacterial zone of the composition against Escherichia coli (mm) and Staphylococcus aureus (mm) is ≥20 mm, having a good antibacterial effect.

[0087] As a possible implementation, the extraction sources of the compound dendrobium extract also include one or more of Dendrobium huoshanense, Dendrobium moniliforme, Dendrobium phalaenopsis, Dendrobium chrysanthum, and Dendrobium loddigesii.

[0088] As a possible implementation, the collagen components include one or more of soluble collagen, soluble collagen cross-linked polymers, collagen, collagen, and small molecule peptides.

[0089] It should be noted that the small molecule peptides mentioned in the context refer to short-chain amino acid polymers with a molecular weight between 180 and 1000 daltons, also known as small peptides, oligopeptides, and oligomeric peptides.

[0090] In some embodiments, by mass, the repair product includes 0.1 to 15 parts of the compound dendrobium extract, 0.1 to 5 parts of Sophora flavescens root extract, 0.1 to 5 parts of Bletilla striata root extract, and 0.1 to 5 parts of collagen components.

[0091] As a non-limiting example, the mass parts of the compound dendrobium extract contained in the repair product can be, but are not limited to, 0.1 part, 0.5 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, or the range between any two of the above mass parts, etc. In some alternative embodiments, the mass parts of the compound dendrobium extract contained in the repair product are 3 to 15 parts.

[0092] The mass parts of the Sophora flavescens root extract contained in the repair product can be, but are not limited to, 0.1 part, 0.5 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, or the range between any two of the above mass parts, etc. In some alternative embodiments, the mass parts of the Sophora flavescens root extract contained in the repair product are 1 to 5 parts.

[0093] The mass parts of the Bletilla striata root extract contained in the repair product can be, but are not limited to, 0.1 part, 0.5 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, or the range between any two of the above mass parts, etc. In some alternative embodiments, the mass parts of the Bletilla striata root extract contained in the repair product are 1 to 5 parts.

[0094] The mass parts of the collagen components contained in the repair product can be, but are not limited to, 0.1 part, 0.5 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, or the range between any two of the above mass parts, etc. In some alternative embodiments, the mass parts of the collagen components contained in the repair product are 1 to 5 parts.

[0095] In the repair-type product, when the dosages of the compound dendrobium extract, sophora flavescens root extract, bletilla striata extract and collagen components are within the above ranges respectively, on the one hand, the repair effect of a single raw material can be enhanced, so that the repair effects achieve a synergistic effect; on the other hand, due to the good antibacterial effect brought by the combined use, the use of traditional preservatives is effectively reduced, making the product milder; in addition, the collagen components can be supplemented to the skin and can also stimulate the synthesis of its own collagen, strengthening the repair effect of the whole combination.

[0096] In some embodiments, the repair-type product further includes a skin conditioner, a humectant, a thickener, an antioxidant, a pH regulator and water.

[0097] In some alternative embodiments, the mass fraction of the skin conditioner is 0.05 parts - 10 parts; for example, it can be, but is not limited to, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts or the range between any two of the above mass fractions, etc.

[0098] As a possible embodiment, the skin conditioner includes one or more of ceramides, phytosterols, fermentation filtrates and plant extracts.

[0099] In some alternative embodiments, the plant extract includes one or more of centella asiatica extract, silybum marianum seed oil, gastrodia elata root extract, salvia miltiorrhiza root extract, thymus vulgaris leaf extract, coptis chinensis root extract, dendrobium wardianum extract, dendrobium phalaenopsis flower extract and dendrobium loddigesii extract.

[0100] In some exemplary embodiments, the mass fraction of the humectant is 0.5 parts - 20 parts; for example, it can be, but is not limited to, 0.5 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts or the range between any two of the above mass fractions, etc.

[0101] In some exemplary embodiments, the humectant includes one or more of polyols such as glycerol, glucosylglycerol, 1,3-propanediol, 1,2-propanediol, butanediol, 1,2-hexanediol, 1,2-pentanediol, dipropylene glycol, sorbitol, sodium hyaluronate, hydrolyzed sodium hyaluronate, sodium polyglutamate, trehalose, erythritol, β-glucan and betaine.

[0102] As a possible embodiment, the mass fraction of the thickener is 0.05 parts - 3 parts; for example, it can be, but is not limited to, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 2 parts, 3 parts or the range between any two of the above mass fractions, etc.

[0103] In some of these embodiments, the thickener includes one or more of carbomer, acrylate / C10-30 alkyl acrylate cross-linked polymer, xanthan gum, Chondrus crispus, sodium alginate, hydroxyethyl cellulose, acryloyldimethyltaurine ammonium / VP copolymer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, polyacrylate cross-linked polymer-6, and hydrolyzed sclerotium gum.

[0104] In some alternative embodiments, the mass fraction of the antioxidant is 0.05 parts - 0.3 parts; for example, it can be, but is not limited to, 0.05 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, or the range between any two of the above mass fractions, etc.

[0105] In some alternative embodiments, the antioxidant includes one or more of vitamin E, tocopheryl acetate, tocotrienol, p-hydroxyacetophenone, and p-hydroxypropiophenone.

[0106] As a possible embodiment, the mass fraction of the pH regulator is 0.05 parts - 3 parts; for example, it can be, but is not limited to, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, or the range between any two of the above mass fractions, etc.

[0107] In some exemplary embodiments, the pH regulator includes one or more of triethanolamine, arginine, sodium hydroxide, and aminomethylpropanol.

[0108] In some embodiments, the type of the repair product includes essence.

[0109] One or more embodiments of the present application provide a preparation method of a repair product, which can prepare the above-mentioned repair product. The preparation method includes the following steps:

[0110] Perform water extraction on Sophora flavescens root powder, Bletilla striata root powder, and a composite Dendrobium composition powder containing Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum respectively to prepare Sophora flavescens root extract, Bletilla striata root extract, and a composite Dendrobium crude extract; mix the composite Dendrobium crude extract with Lactobacillus plantarum and then ferment; perform enzyme removal treatment on the product obtained after fermentation to prepare a composite Dendrobium extract; prepare a mixture containing Sophora flavescens root extract, Bletilla striata root extract, composite Dendrobium extract, and a collagen component to obtain a repair product;

[0111] Wherein, the mass ratio of the composite Dendrobium crude extract to Lactobacillus plantarum is (200 - 600):1; the number-average molecular weight of the composite Dendrobium extract is 6000Da - 8000Da, and the average viscosity at 25°C is 100mpa·s - 200mpa·s.

[0112] As a non-limiting example, the mass ratio of the crude extract of compound dendrobium and Lactobacillus plantarum can be, but is not limited to, 200:1, 220:1, 240:1, 260:1, 280:1, 300:1, 320:1, 340:1, 360:1, 380:1, 400:1, 420:1, 440:1, 460:1, 480:1, 500:1, 520:1, 540:1, 560:1, 580:1, 600:1, or the range between any two of the above ratios, etc.

[0113] After the crude extract of compound dendrobium is fermented, the number-average molecular weight of the dendrobium extract decreases from high to low, and its viscosity changes. The overall average molecular weight changes from high viscosity to low viscosity, which is beneficial for skin absorption and improves the repair ability. And by regulating the mass ratio of the crude extract of compound dendrobium and Lactobacillus plantarum, it is beneficial to obtain a compound dendrobium extract with an appropriate number-average molecular weight and average viscosity.

[0114] This preparation method obtains a compound small-molecule dendrobium extract by optimizing the process. While retaining the active ingredients, it reduces its molecular weight, enhances the skin absorption effect, improves the repair ability, and makes the dendrobium extract more suitable for use by people with sensitive skin. All raw materials used in this preparation method are safe, and natural extraction and purification methods are adopted. Water extraction is used in the extraction process, and no other chemical components and organic solvents are introduced, avoiding the problem of residual organic solvents and chemical components.

[0115] In some embodiments, the fermentation temperature is 26°C - 28°C; for example, it can be, but is not limited to, 26°C, 27°C, 28°C, or the range between any two of the above temperatures, etc.

[0116] As a possible embodiment, the fermentation time is 3 days - 5 days; for example, it can be, but is not limited to, 3 days, 4 days, 5 days, or the range between any two of the above times, etc.

[0117] It should be noted that the fermentation temperature and time can be combined in any suitable way, and both can be selected from any of the fermentation temperatures and times described herein.

[0118] In some embodiments, the single water extraction time for preparing the crude extract of compound dendrobium is 1.5 h - 2 h; for example, it can be, but is not limited to, 1.5 h, 1.6 h, 1.7 h, 1.8 h, 1.9 h, 2 h, or the range between any two of the above times, etc.

[0119] As a possible embodiment, the number of water extraction times for preparing the crude extract of compound dendrobium is 1 time - 4 times; for example, it can be, but is not limited to, 1 time, 2 times, 3 times, 4 times, or the range between any two of the above values, etc.

[0120] When preparing the composite Dendrobium crude extract, controlling the single - time water extraction time and the number of water extraction times within the above - mentioned ranges respectively is beneficial to obtaining a composite Dendrobium extract with an appropriate number - average molecular weight and average viscosity.

[0121] In some embodiments, the water extraction temperature when preparing the composite Dendrobium crude extract is 95°C - 105°C; for example, it can be but is not limited to 95°C, 96°C, 97°C, 98°C, 99°C, 100°C, 101°C, 102°C, 103°C, 104°C, 105°C or the range between any two of the above temperatures, etc.

[0122] As a possible embodiment, the mass ratio of material to water when preparing the composite Dendrobium crude extract is 1:(20 - 40); for example, it can be but is not limited to 1:20, 1:22, 1:24, 1:26, 1:28, 1:30, 1:32, 1:34, 1:36, 1:38, 1:40 or the range between any two of the above mass ratios of material to water, etc.

[0123] In some embodiments, the single - time water extraction time when preparing the Sophora flavescens root extract is 1.5h - 2h; for example, it can be but is not limited to 1.5h, 1.6h, 1.7h, 1.8h, 1.9h, 2h or the range between any two of the above times, etc.

[0124] In some exemplary embodiments, the number of water extraction times when preparing the Sophora flavescens root extract is 1 - 4 times; for example, it can be but is not limited to 1 time, 2 times, 3 times, 4 times or the range between any two of the above values, etc.

[0125] In some alternative embodiments, the water extraction temperature when preparing the Sophora flavescens root extract is 95°C - 105°C; for example, it can be but is not limited to 95°C, 96°C, 97°C, 98°C, 99°C, 100°C, 101°C, 102°C, 103°C, 104°C, 105°C or the range between any two of the above temperatures, etc.

[0126] As a possible embodiment, the mass ratio of material to water when preparing the Sophora flavescens root extract is 1:(20 - 40); for example, it can be but is not limited to 1:20, 1:22, 1:24, 1:26, 1:28, 1:30, 1:32, 1:34, 1:36, 1:38, 1:40 or the range between any two of the above mass ratios of material to water, etc.

[0127] In some embodiments, the single - time water extraction time when preparing the Bletilla striata root extract is 1.5h - 2h; for example, it can be but is not limited to 1.5h, 1.6h, 1.7h, 1.8h, 1.9h, 2h or the range between any two of the above times, etc.

[0128] In some exemplary embodiments, the number of water extraction times for preparing the Bletilla striata root extract is 1 to 4 times; for example, it can be, but is not limited to, 1 time, 2 times, 3 times, 4 times, or the range between any two of the above values, etc.

[0129] In some alternative embodiments, the water extraction temperature for preparing the Bletilla striata root extract is 95°C to 105°C; for example, it can be, but is not limited to, 95°C, 96°C, 97°C, 98°C, 99°C, 100°C, 101°C, 102°C, 103°C, 104°C, 105°C, or the range between any two of the above temperatures, etc.

[0130] As a possible embodiment, the mass-to-water ratio during the preparation of the Bletilla striata root extract is 1:(20 - 40); for example, it can be, but is not limited to, 1:20, 1:22, 1:24, 1:26, 1:28, 1:30, 1:32, 1:34, 1:36, 1:38, 1:40, or the range between any two of the above mass-to-water ratios, etc.

[0131] In some embodiments, the steps of enzyme removal treatment on the product obtained after fermentation include: sequentially performing ethanol enzyme removal and ethanol removal treatment on the product obtained after fermentation.

[0132] In some alternative embodiments, the ethanol removal treatment is carried out by means of reduced-pressure rotary evaporation. Ethanol has a small molecular weight and a low boiling point, is easy to volatilize, and is easy to remove by reduced-pressure rotary evaporation.

[0133] As a possible embodiment, the process parameters of reduced-pressure rotary evaporation include: the temperature is 70°C to 80°C, the rotation speed is 20 rpm to 40 rpm, and the vacuum degree after decompression is 0.05 Pa to 0.095 Pa.

[0134] As a non-limiting example, the temperature of reduced-pressure rotary evaporation can be, but is not limited to, 70°C, 72°C, 74°C, 78°C, 60°C, or the range between any two of the above temperatures, etc. The rotation speed of reduced-pressure rotary evaporation can be, but is not limited to, 20 rpm, 22 rpm, 24 rpm, 26 rpm, 28 rpm, 30 rpm, 32 rpm, 34 rpm, 36 rpm, 38 rpm, 40 rpm, or the range between any two of the above rotation speeds, etc. The vacuum degree after decompression can be, but is not limited to, 0.05 Pa, 0.06 Pa, 0.07 Pa, 0.08 Pa, 0.09 Pa, 0.095 Pa, or the range between any two of the above vacuum degrees, etc.

[0135] In some embodiments, the mass-volume concentrations of Sophora flavescens root extract, Bletilla striata root extract, and compound Dendrobium extract are each independently 25 mg / mL.

[0136] As a possible implementation, the collagen component is a collagen complex, and the collagen complex includes a soluble collagen cross-linked polymer, a filtrate of the fermentation product of Saccharomycopsis galactica, and water.

[0137] In some alternative implementations, the mass proportion of the soluble collagen cross-linked polymer in the collagen complex is 4% - 6%; for example, it can be, but is not limited to, 4%, 5%, 6%, or the range between any two of the above mass proportions, etc.

[0138] In some of these implementations, the mass proportion of the filtrate of the fermentation product of Saccharomycopsis galactica in the collagen complex is 1% - 5%; for example, it can be, but is not limited to, 1%, 2%, 3%, 4%, 5%, or the range between any two of the above mass proportions, etc.

[0139] As a non-limiting example, the steps of separately performing water extraction on Sophora flavescens root powder, Bletilla striata root powder, and a composite Dendrobium composition powder containing Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum to prepare Sophora flavescens root extract, Bletilla striata root extract, and a crude extract of the composite Dendrobium include:

[0140] The Sophora flavescens root powder, Bletilla striata root powder, and the composite Dendrobium composition powder are respectively boiled in water according to a material-to-water ratio of 1:(20 - 40), heated and kept warm at 95°C - 105°C for 1.5 h - 2 h, extracted 1 - 4 times, then filtered through a 200-mesh filter membrane to remove residues, and the filtrate is retained; further centrifuged at 3000 rpm - 5000 rpm for 10 minutes - 15 minutes, the residual precipitate is discarded, the supernatant is taken, and the supernatant is filtered through a 3-μm filter membrane, and the filtrate is collected. The filtrate of the composite Dendrobium is heated and concentrated at 95°C - 105°C until the mass concentration reaches 50 mg / mL to obtain the crude extract of the composite Dendrobium; the filtrates of the Sophora flavescens root and the Bletilla striata root are respectively concentrated until the mass concentration reaches 25 mg / mL to obtain the extract of the Sophora flavescens root and the extract of the Bletilla striata root.

[0141] The following elaborates on the technical solutions of the present invention in combination with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. For the experimental methods without specific conditions noted in the following embodiments, the guidance given in the present invention is preferentially referred to, and it can also be in accordance with the experimental manuals or conventional conditions in the art, or in accordance with the conditions recommended by the manufacturer, or refer to the experimental methods known in the art.

[0142] In the following specific embodiments, regarding the measurement parameters of the raw material components, if there is no special indication, there may be slight deviations within the weighing accuracy range. Regarding the temperature and time parameters, acceptable deviations caused by instrument test accuracy or operation accuracy are allowed.

[0143] It should be noted that the Dendrobium officinale used in the following examples and comparative examples is artificially cultivated Dendrobium officinale.

[0144] I. Preparation of Repairing Essence

[0145] Example 1

[0146] Step S1: Wash Dendrobium officinale, Dendrobium nobile, and Dendrobium fimbriatum separately with deionized water, dry for 4 hours, pulverize, and pass through a 60-mesh sieve to obtain Dendrobium officinale powder, Dendrobium nobile powder, and Dendrobium fimbriatum powder; mix the three kinds of Dendrobium powders in a ratio of 1:1:1 to obtain a composite Dendrobium composition powder.

[0147] Step S2: Wash Sophora flavescens root and Bletilla striata root separately with deionized water, dry for 4 hours, pulverize, and pass through a 60-mesh sieve to obtain Sophora flavescens root powder and Bletilla striata powder.

[0148] Step S3: Boil the composite Dendrobium composition powder, Sophora flavescens root powder, and Bletilla striata root powder respectively according to a material-to-water ratio of 1:40, keep warm and heat at 100 °C for 4 hours, extract 4 times, then filter out the residue with a 200-mesh filter membrane and retain the filtrate. Further impurity removal is carried out by centrifuging at 5000 r / min for 10 minutes with a centrifuge, discard the remaining precipitate, take the supernatant, and filter the supernatant through a 3-μm filter membrane to collect the filtrate.

[0149] Step S4: Heat and concentrate the filtrate obtained in Step S3 respectively, control the temperature at 100 °C, concentrate the composite Dendrobium filtrate to a concentration of 50 mg / mL to obtain a composite Dendrobium crude extract, and concentrate the Sophora flavescens root filtrate and Bletilla striata filtrate to 25 mg / mL to obtain Sophora flavescens root extract and Bletilla striata extract, and set aside.

[0150] Step S5: Add Lactobacillus plantarum to the composite Dendrobium crude extract, add it according to a mass ratio of the composite Dendrobium crude extract to Lactobacillus plantarum of 200:1, control the temperature at 28 °C, and carry out constant-temperature fermentation for 5 days. Stop fermentation after observing bubbles on its surface.

[0151] Step S6: Add 95% ethanol to the fermentation product and filter to remove the enzyme.

[0152] Step S7: Remove ethanol from the material processed in Step 6 by means of vacuum rotary evaporation, the speed of vacuum rotary evaporation is 30 rpm, the temperature of vacuum rotary evaporation is controlled at 75 °C, and the vacuum degree after decompression is 0.09 Pa; concentrate the composite Dendrobium extract to 25 mg / ml.

[0153] Step S8: Take 15 parts by mass of compound dendrobium extract, 5 parts of sophora flavescens root extract, 5 parts of bletilla striata root extract, 5 parts of collagen component (water / soluble collagen cross-linked polymer / galactomyces ferment filtrate), 5 parts of humectant 1,3-butanediol, 0.05 part of humectant sodium hyaluronate, 0.05 part of humectant 1,2-hexanediol, 0.05 part of antioxidant p-hydroxyacetophenone, 0.35 part of thickener acrylate / C10-30 alkanol acrylate cross-linked polymer, 0.35 part of pH regulator arginine, 3 parts of skin conditioner gastrodia elata root extract, 2 parts of skin conditioner salvia miltiorrhiza root extract and 0.1 part of antioxidant tocopherol. Add deionized water to make up 100 parts to obtain a repair essence; among them, the mass ratio of the soluble collagen cross-linked polymer in the collagen component is 5%, and the mass ratio of the galactomyces ferment filtrate is 3%.

[0154] Example 2

[0155] The preparation method of Example 2 is similar to that of Example 1, the difference is that: in step S3 of Example 2, the heating time for water extraction is 4 hours and the extraction times are 2 times; the others are the same.

[0156] Example 3

[0157] The preparation method of Example 3 is similar to that of Example 1, the difference is that: in step S3 of Example 3, the heating time for water extraction is 4 hours and the extraction times are 1 time; the others are the same.

[0158] Example 4

[0159] The preparation method of Example 4 is similar to that of Example 1, the difference is that: in step S3 of Example 4, the heating time for water extraction is 2 hours and the extraction times are 4 times; the others are the same.

[0160] Example 5

[0161] The preparation method of Example 5 is similar to that of Example 1, the difference is that: in step S3 of Example 5, the heating time for water extraction is 1 hour and the extraction times are 4 times; the others are the same.

[0162] Example 6

[0163] The preparation method of Example 6 is similar to that of Example 1, the difference is that: in step S5 of Example 6, the mass ratio of the compound dendrobium extract to lactobacillus plantarum during fermentation is 400:1, and the fermentation is carried out for 5 days; the others are the same.

[0164] Example 7

[0165] The preparation method of Example 7 is similar to that of Example 1, except that: in step S5 of Example 7, when fermentation is carried out, the mass ratio of the compound dendrobium extract to Lactobacillus plantarum is 600:1, and the fermentation lasts for 5 days; others are the same.

[0166] Example 8

[0167] The preparation method of Example 8 is similar to that of Example 1, except that: in step S8 of Example 8, 7.5 parts of the compound dendrobium extract, 2.5 parts of Sophora flavescens root extract, and 2.5 parts of Bletilla striata root extract are used; others are the same.

[0168] Example 9

[0169] The preparation method of Example 9 is similar to that of Example 1, except that: in step S8 of Example 9, 3 parts of the compound dendrobium extract, 1 part of Sophora flavescens root extract, and 1 part of Bletilla striata root extract are used; others are the same.

[0170] Example 10

[0171] The preparation method of Example 10 is similar to that of Example 1, except that: in the compound dendrobium composition powder in step S1 of Example 10, the mass ratio of Dendrobium officinale powder, Dendrobium nobile Lindl. powder, and Dendrobium fimbriatum Hook. powder is 2:1:1; others are the same.

[0172] Example 11

[0173] The preparation method of Example 11 is similar to that of Example 1, except that: in the compound dendrobium composition powder in step S1 of Example 11, the mass ratio of Dendrobium officinale powder, Dendrobium nobile Lindl. powder, and Dendrobium fimbriatum Hook. powder is 1:2:2; others are the same.

[0174] Example 12

[0175] The preparation method of Example 12 is similar to that of Example 1, except that: in the compound dendrobium composition powder in step S1 of Example 12, the mass ratio of Dendrobium officinale powder, Dendrobium nobile Lindl. powder, and Dendrobium fimbriatum Hook. powder is 2:1:2; others are the same.

[0176] Example 13

[0177] The preparation method of Example 13 is similar to that of Example 1, except that: in the compound dendrobium composition powder in step S1 of Example 13, the mass ratio of Dendrobium officinale powder, Dendrobium nobile Lindl. powder, and Dendrobium fimbriatum Hook. powder is 2:2:1; others are the same.

[0178] Comparative Example 1

[0179] The preparation method of Comparative Example 1 is similar to that of Example 9, except that: in step S8 of Comparative Example 1, 3 parts of the compound dendrobium extract and 1 part of Bletilla striata root extract are used, and no Sophora flavescens root extract is contained; others are the same.

[0180] Comparative Example 2

[0181] The preparation methods of Comparative Example 2 and Example 9 are similar, with the difference that: in step S8 of Comparative Example 2, 3 parts of compound dendrobium extract and 1 part of sophora flavescens root extract are used, and it does not contain bletilla striata root extract; the others are the same.

[0182] Comparative Example 3

[0183] The preparation methods of Comparative Example 3 and Example 9 are similar, with the difference that: in step S8 of Comparative Example 3, 3 parts of compound dendrobium extract are used, and it does not contain sophora flavescens root extract and bletilla striata root extract; the others are the same.

[0184] Comparative Example 4

[0185] The preparation methods of Comparative Example 4 and Example 1 are similar, with the difference that: in step S8 of Comparative Example 4, collagen components (water / soluble collagen cross-linked polymer / galactomyces ferment filtrate) are not added; the others are the same.

[0186] Comparative Example 5

[0187] The preparation methods of Comparative Example 5 and Example 1 are similar, with the difference that: in step S4 of Comparative Example 5, the compound dendrobium filtrate is concentrated to 25 mg / mL, and the fermentation treatment in step S5 is not carried out; the others are the same.

[0188] Comparative Example 6

[0189] The preparation methods of Comparative Example 6 and Example 1 are similar, with the difference that: in step S5 of Comparative Example 6, the mass ratio of the compound dendrobium extract to lactobacillus plantarum during fermentation is 650:1, and the fermentation is carried out for 5 days; the others are the same.

[0190] Comparative Example 7

[0191] The preparation methods of Comparative Example 7 and Example 1 are similar, with the difference that: in step S5 of Comparative Example 7, the mass ratio of the compound dendrobium extract to lactobacillus plantarum during fermentation is 150:1, and the fermentation is carried out for 5 days; the others are the same.

[0192] II. Performance Test

[0193] 1. The number-average molecular weight of the compound dendrobium extract in Examples 1-7 and Comparative Examples 5-7 was measured by the GPC method (gel permeation chromatography).

[0194] The Waters 515 gel chromatograph was used, equipped with an Ultrahydrogel chromatographic column, to calculate the number-average molecular weight and distribution of the compound dendrobium extract. The experimental conditions are as follows:

[0195] RI flow cell temperature: 40 °C; mobile phase: 50 mM acetic acid + 100 mM sodium nitrate buffer; flow rate: 0.42 mL / min; chromatographic column: Waters Ultrahydrogel 2000 column, 7.8 X 300 mm; column temperature: 40 °C; sample diluent: 50 mM acetic acid + 100 mM sodium nitrate buffer; injection volume: 50 μL; data processing software: Empower QS + GPC calculation module chromatographic data software; sample preparation: Dilute the compound dendrobium solution from 25 mg / mL to 1 mg / mL. The experimental results are shown in Table 1 and Figure 1 as follows.

[0196] Table 1

[0197]

[0198] It can be seen from the result comparison of Examples 1-5 in Table 1 that by regulating the heating time and extraction times of single water extraction within an appropriate range, it is beneficial to reduce the number average molecular weight of the compound dendrobium extract, making the number average molecular weight of the compound dendrobium extract between 6 KDa and 8 KDa.

[0199] It can be seen from the result comparison of Example 1, Examples 6-7 and Comparative Examples 5-7 that after fermentation, the number average molecular weight of the compound dendrobium extract is greatly reduced; and by controlling the mass ratio of the initial extract of compound dendrobium to Lactobacillus plantarum during fermentation within the range of (200 - 600):1, it is beneficial to regulate the number average molecular weight of the compound dendrobium extract between 6 KDa and 8 KDa; in addition, as the addition amount of Lactobacillus plantarum decreases, the reduction of the number average molecular weight of the compound dendrobium extract is relatively slow.

[0200] 2. The average viscosity of the compound dendrobium extracts in Examples 1-7 and Comparative Examples 5-7 was measured by the viscosity method.

[0201] Testing instrument: AMETEK BROOKFIELD / Brookfield DV2T viscometer; rotor speed RV1 #20 r / min; test solution: 25 mg / mL compound dendrobium extract solution; test temperature: 25 °C. The test results are shown in Table 2 and Figure 2 as follows.

[0202] Table 2

[0203]

[0204] It can be seen from the result comparison of Examples 1-5 in Table 2 that by regulating the heating time and extraction times of single water extraction within an appropriate range, it is beneficial to reduce the average viscosity of the compound dendrobium extract, making the average viscosity of the compound dendrobium extract between 100 mPa·s and 200 mPa·s.

[0205] From the result comparison of Example 1, Examples 6-7 and Comparative Examples 5-7, it can be seen that after fermentation, the average viscosity of the compound dendrobium extract is greatly reduced; and by controlling the mass ratio of the initial dendrobium extract to Lactobacillus plantarum during fermentation within the range of (200-600):1, it is beneficial to regulate the average viscosity of the compound dendrobium extract between 100 mPa·s and 200 mPa·s; in addition, as the addition amount of Lactobacillus plantarum decreases, the decrease in the average viscosity of the compound dendrobium extract is relatively slow.

[0206] 3. Inhibition zone test

[0207] The antibacterial activities of the repair essence prepared in Example 1, Examples 8-13 and Comparative Examples 1-3 against Escherichia coli and Staphylococcus aureus were investigated by the inhibition zone method to evaluate their antibacterial efficacy. Among them, the essence without compound dendrobium extract, sophora flavescens root extract and bletilla striata root extract was used as the blank control compared with Example 1. The investigation results are shown in Table 3 and Figure 3 as follows.

[0208] Table 3

[0209]

[0210] From the result comparison of Example 1, Examples 8-13 and Comparative Example 3 in Table 3, it can be seen that the essence containing compound dendrobium extract, sophora flavescens root extract and bletilla striata root extract at the same time has a significantly better inhibition zone diameter than the essence only using compound dendrobium extract, and as the addition amount of the extract increases, the inhibition zone diameter increases significantly.

[0211] From the results of Comparative Examples 1-3, it can be seen that sophora flavescens root extract has a significant inhibitory effect on Escherichia coli, bletilla striata root extract has a significant inhibitory effect on Staphylococcus aureus, and compound dendrobium extract has a significant inhibitory effect on both Escherichia coli and Staphylococcus aureus. From the result comparison of Example 1 and Comparative Examples 1-3, it can be seen that compound dendrobium extract, sophora flavescens root extract and bletilla striata root extract have a synergistic antibacterial effect.

[0212] 4. Hyaluronidase inhibition test

[0213] Hyaluronidase is the main component of the extracellular matrix of connective tissue cells in the body. Excessive hyaluronidase will promote allergic reactions and is related to most type I allergic reactions mediated by IgE and type IV allergic reactions mediated by T cells. The increase of IgE will promote the release of inflammatory factors and cause allergic phenomena.

[0214] According to GDCTC / OT-L014 "Cosmetics - Operating Procedures for Hyaluronidase Inhibition Test", the hyaluronidase inhibition test was carried out on the repair essence obtained in Example 1, Examples 8 - 13 and Comparative Examples 1 - 4. Among them, compared with Example 1, the essence without compound dendrobium extract, sophora flavescens root extract and bletilla striata root extract and the essence with collagen components were used as blank controls. The inspection results are shown in Table 4 and Figure 4 0000443as follows. 0000444

[0215] 0000445Table 4 0000446

[0216] 0000447 0000448 0000449

[0217] 0000450It can be seen from the result comparison of Example 1, Examples 8 - 13 and Comparative Example 3 in Table 4 that the essence containing compound dendrobium extract, sophora flavescens root extract and bletilla striata root extract at the same time has significantly better hyaluronidase inhibition effect than the essence only using compound dendrobium extract, and with the increase of the addition amount of the extract, the hyaluronidase inhibition rate increases significantly. 0000451

[0218] 0000452It can be seen from the result comparison of Example 1 and Comparative Examples 1 - 3 that compound dendrobium extract, sophora flavescens root extract and bletilla striata root extract have a synergistic hyaluronidase inhibition effect, that is, a synergistic soothing effect. 0000453

[0219] 0000454It can be seen from the result comparison of Example 1 and Comparative Example 4 that adding collagen components (water / soluble collagen cross-linked polymer / galactomyces ferment filtrate) is beneficial to inhibiting hyaluronidase, indicating that adding collagen components helps to enhance the soothing effect. 0000455

[0220] 0000456The technical features of the above-mentioned embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification. 0000457

[0221] 0000458The above-mentioned embodiments only express several implementation manners of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent should be subject to the appended claims.

Claims

1. A repair composition, characterized in that: The invention comprises a composite dendrobium extract, a sophora flavescens root extract and a bletilla striata root extract; the number average molecular weight of the composite dendrobium extract is 6000Da-8000Da, and the average viscosity at 25°C is 100mpa·s-200mpa·s; the extraction sources of the composite dendrobium extract include dendrobium officinale, dendrobium nobile and dendrobium tassellensis.

2. The repair composition according to claim 1, characterized in that The mass ratio of the composite dendrobium extract, the Sophora flavescens root extract and the Bletilla striata root extract is (1-5):(1-5):(1-5).

3. The repair composition according to any one of claims 1 to 2, characterized in that: The mass ratio of the Dendrobium officinale, the Dendrobium nobile and the Dendrobium tasseliensis in the extraction sources of the composite Dendrobium extract is (1-2):(1-2):(1-2); Optionally, the extraction source of the composite Dendrobium extract also includes one or more of Dendrobium huoshanense, Dendrobium slender-stemmed, Dendrobium huoshanense, Dendrobium huangcao and Dendrobium huancao.

4. A repair product, characterized in that: It includes a repairing composition and a collagen component, wherein the repairing composition includes a composite dendrobium extract, a sophora flavescens root extract and a bletilla striata root extract; the number average molecular weight of the composite dendrobium extract is 6000Da-8000Da, and the average viscosity at 25°C is 100mpa·s-200mpa·s; the extraction sources of the composite dendrobium extract include Dendrobium officinale, Dendrobium nobile and Dendrobium tasseliensis.

5. The repair product according to claim 4, characterized in that: The mass ratio of the composite dendrobium extract, the Sophora flavescens root extract and the Bletilla striata root extract is (1-5):(1-5):(1-5); and / or The mass ratio of the Dendrobium officinale, the Dendrobium nobile and the Dendrobium tasseliensis in the extraction sources of the composite Dendrobium extract is (1-2):(1-2):(1-2); Optionally, the composite dendrobium extract may be extracted from one or more of Dendrobium huoshanense, Dendrobium slender-stemmed, Dendrobium huoshanense, Dendrobium huangcao and Dendrobium huancao.

6. The repair product according to claim 4, characterized in that: The collagen components include one or more of soluble collagen, soluble collagen cross-linked polymer, collagen protein, collagen and small molecule peptides.

7. The repair product according to claim 4, characterized in that: In parts by mass, the repair product comprises 0.1 to 15 parts of a composite dendrobium extract, 0.1 to 5 parts of a sophora flavescens root extract, 0.1 to 5 parts of a bletilla striata root extract, and 0.1 to 5 parts of a collagen component; Optionally, the repair product includes, by weight, 3-15 parts of composite dendrobium extract, 1-5 parts of Sophora flavescens root extract, 1-5 parts of Bletilla striata root extract and 1-5 parts of collagen components.

8. The repair product according to any one of claims 4 to 7, characterized in that: The repair product also includes a skin conditioner, a moisturizer, a thickener, an antioxidant, a pH adjuster and water; Optionally, the mass fraction of the skin conditioning agent is 0.05 parts to 10 parts; Optionally, the mass fraction of the moisturizing agent is 0.5 parts to 20 parts; Optionally, the mass fraction of the thickener is 0.05 parts to 3 parts; Optionally, the antioxidant is present in an amount of 0.05 to 0.3 parts by weight; Optionally, the mass fraction of the pH adjuster is 0.05 parts to 3 parts; Optionally, the moisturizing agent includes one or more of glycerol, glyceryl glucoside, 1,3-propylene glycol, 1,2-propylene glycol, butylene glycol, 1,2-hexanediol, 1,2-pentanediol, dipropylene glycol and other polyols, sorbitol, sodium hyaluronate, hydrolyzed sodium hyaluronate, sodium polyglutamate, trehalose, erythritol, β-glucan and betaine; Optionally, the thickener includes one or more of carbomer, acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, xanthan gum, crispy chondrus crispus, sodium alginate, hydroxyethyl cellulose, ammonium acryloyldimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polyacrylate crosspolymer-6 and hydrolyzed sclerotium gum; Optionally, the antioxidant includes one or more of vitamin E, tocopherol acetate, tocopherol, p-hydroxyacetophenone and p-hydroxypropiophenone; Optionally, the pH regulator includes one or more of triethanolamine, arginine, sodium hydroxide and aminomethyl propanol; Optionally, the skin conditioning agent comprises one or more of ceramides, phytosterols, fermentation filtrates, and plant extracts; Further optionally, the plant extract includes one or more of Centella asiatica extract, milk thistle seed oil, Gastrodia elata root extract, Salvia miltiorrhiza root extract, Thyme leaf extract, Coptis chinensis root extract, Dendrobium officinale extract, Dendrobium leucoderma flower extract and Dendrobium annuum extract.

9. The repair product according to any one of claims 4 to 7, characterized in that: The types of the repair products include essences.

10. A method for preparing a repair product, characterized in that: The steps include: Extracting the powder of Sophora flavescens root, the powder of Bletilla striata root and the powder of the composite Dendrobium composition comprising Dendrobium officinale, Dendrobium nobile and Dendrobium tasseliensis respectively with water to prepare the extract of Sophora flavescens root, the extract of Bletilla striata root and the composite Dendrobium primary extract; The composite dendrobium primary extract is mixed with Lactobacillus plantarum and then fermented; The product obtained after fermentation is subjected to enzyme removal treatment to prepare a composite dendrobium extract; preparing a mixed solution comprising the Sophora flavescens root extract, the Bletilla striata root extract, the composite Dendrobium officinale extract and collagen components to obtain the repair product; The mass ratio of the composite dendrobium primary extract to the plant lactobacillus is (200-600):1; the number average molecular weight of the composite dendrobium extract is 6000Da-8000Da, and the average viscosity at 25°C is 100mpa·s-200mpa·s.

11. The preparation method according to claim 10, characterized in that: The preparation method comprises at least one of the following conditions: (1) The fermentation temperature is 26° C.-28° C. and the fermentation time is 3 to 5 days; (2) When preparing the composite dendrobium primary extract, the single water extraction time is 1.5h-2h, the number of water extractions is 1-4 times, the water extraction temperature is 95°C-105°C, and the material-water mass ratio is 1:(20-40); (3) When preparing the Sophora flavescens root extract, the single water extraction time is 1.5h-2h, the number of water extractions is 1-4 times, the water extraction temperature is 95°C-105°C, and the material-water mass ratio is 1:(20-40); (4) When preparing the Bletilla striata root extract, the single water extraction time is 1.5h-2h, the number of water extractions is 1-4 times, the water extraction temperature is 95°C-105°C, and the material-water mass ratio is 1:(20-40); (5) The step of subjecting the fermentation product to enzyme removal treatment comprises: subjecting the fermentation product to ethanol enzyme removal and ethanol removal treatment in sequence; Optionally, the ethanol removal treatment is performed by vacuum rotary evaporation; Optionally, the process parameters of the reduced pressure rotary evaporation include: temperature of 70°C-80°C, rotation speed of 20rpm-40rpm, and vacuum degree of 0.05Pa-0.095Pa after reduced pressure; (6) the mass volume concentration of the Sophora flavescens root extract, the Bletilla striata root extract and the composite Dendrobium officinale extract are each independently 25 mg / mL; (7) The collagen component is a collagen complex, which includes a soluble collagen cross-linked polymer, a filtrate of a galactosidase-like bacteria fermentation product, and water; Optionally, the mass proportion of the soluble collagen cross-linked polymer in the collagen complex is 4%-6%; the mass proportion of the filtrate of the galactose yeast-like bacteria fermentation product in the collagen complex is 1%-5%.

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