Uric acid reducing composition containing eucommia polysaccharide as well as preparation method and application of uric acid reducing composition

Abstract

The invention discloses a uric acid reducing composition containing eucommia polysaccharide as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The uric acid reducing composition prepared by the invention is prepared from the eucommia polysaccharide, the mulberry leaf extract and the clove extract, and the eucommia polysaccharide, the mulberry leaf extract and the clove extract are compounded for use, so that generation-excretion dual blocking can be formed aiming at different links of uric acid metabolism, and multi-target synergistic uric acid reduction is realized. Meanwhile, plant-derived components are adopted, so that the safety is higher, and adverse reactions can be effectively reduced. Besides, lactobacillus sake, lactobacillus plantarum and kluyveromyces marxianus are adopted as fermentation strains to prepare the mulberry leaf extract through fermentation, and the content of effective components in the mulberry leaf extract can be effectively increased, so that the uric acid reducing effect of the prepared composition is improved.

Description

Technical Field

[0001] The present invention relates to the field of biological medicine technology, and particularly to a uric acid-lowering composition containing eucommia ulmoides polysaccharide, a preparation method thereof, and an application thereof. Background Art

[0002] Hyperuricemia (HUA) refers to the situation where, in adults under normal purine diet, the fasting blood uric acid level is > 420 μmol / L on two non-consecutive days. An increase in blood uric acid level is not only associated with the occurrence and development of diseases in systems such as the kidneys, endocrine metabolism, and cardiovascular and cerebrovascular systems, but also finally leads to renal fibrosis and renal failure, endangering life and health.

[0003] Currently, drugs are mostly taken to lower uric acid, thereby achieving effective treatment of hyperuricemia. Among them, the uric acid-lowering drugs are mainly Western medicines. For example, allopurinol, febuxostat, and benzbromarone, etc. These Western medicines achieve the effect of reducing blood uric acid and treating hyperuricemia by promoting uric acid excretion and inhibiting uric acid synthesis. However, long-term use may have adverse reactions such as liver and kidney damage, allergic reactions, and cardiovascular risks, thereby affecting the treatment effect. In sharp contrast, plant-derived extracts, as natural substances, due to their high safety and few toxic and side effects, show unique advantages and potential in the field of uric acid lowering and have gradually become a research hotspot.

[0004] In the prior art, CN115607634A discloses a traditional Chinese medicine composition for preventing and treating hyperuricemia and gout and its application. The traditional Chinese medicine composition is prepared from the following raw materials: coix seed, polygonatum sibiricum, mulberry leaf, papaya, and walnut kernel. However, it is only prepared by mixing the above-mentioned traditional Chinese medicine components and decocting them in hot water. The content of active ingredients in the traditional Chinese medicine composition prepared by this hot water extraction method is small, and the active ingredients may also volatilize, deform, or decompose during hot water extraction, resulting in poor effect of the traditional Chinese medicine composition in lowering uric acid. CN113456661A discloses a uric acid-lowering composite polysaccharide composition and its application. The composite polysaccharide composition includes an inonotus obliquus polysaccharide extract, a morchella esculenta polysaccharide extract, and a hericium erinaceus polysaccharide extract. Although this composition has xanthine inhibitory activity and uric acid-lowering activity, it is only composed of polysaccharide substances, and the components are relatively single. Uric acid lowering involves multiple physiological links. Relying solely on polysaccharide substances to regulate the activity of uric acid metabolism-related enzymes and play anti-inflammatory and immunomodulatory roles is difficult to comprehensively cover all factors affecting uric acid levels.

[0005] Therefore, it is particularly important to find plant-derived extracts with synergistic effects to prepare a composition that can effectively reduce uric acid. Summary of the Invention

[0006] In view of the above-mentioned prior art, the object of the present invention is to provide a uric acid-lowering composition containing eucommia ulmoides polysaccharide, and its preparation method and application. The uric acid-lowering composition prepared by the present invention is made of eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract. The compound use of eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract can form a "generation-excretion" dual block for different links of uric acid metabolism, and achieve multi-target synergistic uric acid reduction. At the same time, compared with chemical drugs such as allopurinol, the present invention adopts plant-derived components, has higher safety, and can effectively reduce the occurrence of adverse reactions.

[0007] To achieve the above object, the present invention adopts the following technical solutions:

[0008] In the first aspect of the present invention, a uric acid-lowering composition containing eucommia ulmoides polysaccharide is provided, and the uric acid-lowering composition is prepared from eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract according to a weight ratio of (8-12):(4-6):(4-6).

[0009] Preferably, the eucommia ulmoides polysaccharide is prepared by the following method:

[0010] After mixing eucommia ulmoides leaf powder and water according to a solid-liquid ratio of 1 g:(5-15) mL, ultrasonic treatment is carried out and then centrifuged, the filtrate is collected and concentrated to obtain a concentrated solution; after mixing the concentrated solution and absolute ethanol according to a volume ratio of 1:(2-5), standing is carried out and then filtered, the precipitate is collected, and after purification and drying, eucommia ulmoides polysaccharide is obtained.

[0011] Further preferably, during the ultrasonic treatment, the ultrasonic temperature is 50-70 °C, the ultrasonic power is 200-400 W, and the ultrasonic time is 0.5-1 h.

[0012] Further preferably, the centrifugation speed is 3500-4500 r / min, and the centrifugation time is 8-12 min.

[0013] Further preferably, the standing time is 24 h.

[0014] Preferably, the mulberry leaf extract is prepared by the following method:

[0015] After mixing the seed liquid of Lactobacillus sakei, the seed liquid of Kluyveromyces marxianus and the seed liquid of Lactobacillus plantarum according to a volume ratio of 1:(1.5-2.5):(4-5), a mixed seed liquid is obtained; the mixed seed liquid is inoculated into a mulberry leaf liquid medium, and after fermentation culture and centrifugation, a fermentation broth is obtained; the fermentation broth is concentrated and dried to obtain the mulberry leaf extract.

[0016] Further preferably, the preservation number of Lactobacillus sakei is ACCC 05429, the preservation number of Kluyveromyces marxianus is ACCC 20314, and the preservation number of Lactobacillus plantarum is ACCC 03954.

[0017] Further preferably, in the mixed seed liquid, the viable count of Lactobacillus sakei ≥ 1.8×10 6 cfu / mL, the viable count of Kluyveromyces marxianus ≥ 1.8×10 6 cfu / mL, and the viable count of Lactobacillus plantarum ≥ 1.2×10 7 cfu / mL.

[0018] Further preferably, the components of the mulberry leaf liquid medium are: (180 - 220) g / L potato leachate, (15 - 25) g / L sucrose, and (25 - 35) mg / L mulberry leaf extract;

[0019] The preparation method of the mulberry leaf extract is as follows: Mix mulberry leaves and water according to a material - liquid ratio of 1 g:(15 - 25) mL, heat and decoct 2 - 3 times, with each decoction time being 3 - 4 h, combine the decoction liquids, and dry to obtain the mulberry leaf extract.

[0020] Further preferably, the inoculation amount of the mixed seed liquid is 8 - 12% of the volume of the fermentation medium.

[0021] Further preferably, during the fermentation culture process, the temperature is 30 - 37 °C and the fermentation time is 5 - 7 d.

[0022] Preferably, the clove extract is prepared by the following method:

[0023] Mix cloves and ethanol with a mass fraction of 75% - 90% according to a material - liquid ratio of 1 g:(4 - 6) mL, perform ultrasonic extraction at a power of 300 - 400 W for 2 - 3 h, filter, collect the supernatant, and concentrate and dry it to obtain the clove extract.

[0024] In the second aspect of the present invention, a preparation method of the above uric - acid - lowering composition containing eucommia ulmoides polysaccharide is provided, including the following steps:

[0025] Mix eucommia ulmoides polysaccharide, mulberry leaf extract, and clove extract evenly to obtain the uric - acid - lowering composition containing eucommia ulmoides polysaccharide.

[0026] In the third aspect of the present invention, an application of the above uric - acid - lowering composition containing eucommia ulmoides polysaccharide in the preparation of uric - acid - lowering products is provided.

[0027] The beneficial effects of the present invention:

[0028] The uric acid-lowering composition prepared by the present invention is made of eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract. The eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract are used in combination, and can form a "generation-excretion" dual block targeting different links of uric acid metabolism, achieving multi-target synergistic uric acid reduction. At the same time, compared with chemical drugs such as allopurinol, the present invention uses plant-derived ingredients, has higher safety, and can effectively reduce the occurrence of adverse reactions. In addition, the present invention uses Lactobacillus sakei, Lactobacillus plantarum and Kluyveromyces marxianus as fermentation strains to ferment and prepare mulberry leaf extract. Compared with methods such as hot water extraction and ultrasonic microwave extraction, the content of active ingredients in the mulberry leaf extract prepared by fermentation is higher, thereby further improving the effect of the uric acid-lowering composition in reducing blood uric acid. Detailed Description of the Invention

[0029] It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present application belongs.

[0030] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.

[0031] The experimental materials used in the embodiments of the present invention are all conventional experimental materials in the art and can be obtained through commercial channels.

[0032] Among them, Lactobacillus sakei, Kluyveromyces marxianus and Lactobacillus plantarum are all purchased from the China Center for Type Culture Collection of Agricultural Microorganisms. The preservation number of Lactobacillus sakei is ACCC 05429, the preservation number of Kluyveromyces marxianus is ACCC 20314, and the preservation number of Lactobacillus plantarum is ACCC 03954.

[0033] The potato leaching solution is prepared by the following method: after peeling the potatoes, 200 g of potatoes are cut into small pieces, put into 800 mL of distilled water, boiled and then boiled for 20-30 min, and filtered through 4-6 layers of gauze to remove the potato residue to obtain the potato leaching solution.

[0034] Example 1: Uric acid-lowering composition containing eucommia ulmoides polysaccharide

[0035] The preparation method of eucommia ulmoides polysaccharide is as follows:

[0036] Dry Eucommia ulmoides leaves and crush them to obtain Eucommia ulmoides leaf powder; mix the Eucommia ulmoides leaf powder and water at a material-liquid ratio of 1 g: 10 mL, perform ultrasonic treatment at 60 °C and 300 W for 45 min, then centrifuge at 4000 r / min for 10 min, collect the filtrate and concentrate it to obtain a concentrated solution; mix the concentrated solution and absolute ethanol at a volume ratio of 1: 3.5, let it stand for 24 h, filter, collect the precipitate after filtration, perform deproteinization and decolorization for purification and drying to obtain Eucommia ulmoides polysaccharide.

[0037] The preparation method of mulberry leaf extract is as follows:

[0038] Mix the seed liquid of Lactobacillus sakei, the seed liquid of Kluyveromyces marxianus and the seed liquid of Lactobacillus plantarum at a volume ratio of 1: 2: 4.5 to obtain a mixed seed liquid, and adjust the viable count of Lactobacillus sakei in the mixed seed liquid to 1.8×10 6 cfu / mL, the viable count of Kluyveromyces marxianus is 1.8×10 6 cfu / mL, and the viable count of Lactobacillus plantarum is 1.2×10 7 cfu / mL;

[0039] Inoculate the mixed seed liquid into the mulberry leaf liquid medium, where the inoculation amount of the mixed seed liquid is 10% of the volume of the mulberry leaf liquid medium, ferment at 35 °C for 6 d, centrifuge to obtain a fermentation broth; concentrate and dry the fermentation broth to obtain mulberry leaf extract;

[0040] The components of the mulberry leaf liquid medium are: 30 mg / L mulberry leaf extract, 200 g / L potato leaching solution, 20 g / L sucrose; among them, the mulberry leaf extract is prepared by the following method: mix mulberry leaves and water at a material-liquid ratio of 1 g: 20 mL, heat and decoct 3 times, each decocting time is 3.5 h, combine the decoction liquids, dry to obtain mulberry leaf extract.

[0041] The preparation method of clove extract is as follows:

[0042] Dry cloves and crush them, mix them with ethanol with a mass fraction of 80% at a material-liquid ratio of 1 g: 5 mL, perform ultrasonic extraction at 350 W for 2.5 h, filter, collect the supernatant, concentrate and dry to obtain clove extract.

[0043] Mix Eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract at a weight ratio of 10: 5: 5 to obtain a uric acid-lowering composition containing Eucommia ulmoides polysaccharide.

[0044] Example 2:

[0045] The preparation method of Eucommia ulmoides polysaccharide is as follows:

[0046] Dry Eucommia ulmoides leaves and then crush them to obtain Eucommia ulmoides leaf powder; mix the Eucommia ulmoides leaf powder and water at a material-liquid ratio of 1 g: 5 mL, subject to ultrasonic treatment at 50 °C and 200 W for 1 h, then centrifuge at 3500 r / min for 12 min, collect the filtrate and concentrate it to obtain a concentrated solution; mix the concentrated solution and absolute ethanol at a volume ratio of 1:5, let it stand for 24 h, filter, collect the precipitate after filtration, perform deproteinization and decolorization for purification and drying to obtain Eucommia ulmoides polysaccharide.

[0047] The preparation method of mulberry leaf extract is as follows:

[0048] Mix the Leuconostoc sake seed liquid, Kluyveromyces marxianus seed liquid and Lactobacillus plantarum seed liquid at a volume ratio of 1:1.5:4 to obtain a mixed seed liquid, and adjust the viable count of Leuconostoc sake in the mixed seed liquid to 1.8×10 6 cfu / mL, the viable count of Kluyveromyces marxianus is 1.8×10 6 cfu / mL, and the viable count of Lactobacillus plantarum is 1.2×10 7 cfu / mL;

[0049] Inoculate the mixed seed liquid into the mulberry leaf liquid medium, wherein the inoculation amount of the mixed seed liquid is 8% of the volume of the mulberry leaf liquid medium, ferment at 30 °C for 5 d, centrifuge to obtain a fermentation broth; concentrate and dry the fermentation broth to obtain mulberry leaf extract;

[0050] The components of the mulberry leaf liquid medium are: 25 mg / L mulberry leaf extract, 180 g / L potato leaching solution, 15 g / L sucrose; among them, the mulberry leaf extract is prepared by the following method: mix mulberry leaves and water at a material-liquid ratio of 1 g: 15 mL, heat and decoct 3 times, each decoction time is 3 h, combine the decoction liquids, dry to obtain mulberry leaf extract.

[0051] The preparation method of clove extract is as follows:

[0052] Dry cloves and crush them, mix them with ethanol with a mass fraction of 75% at a material-liquid ratio of 1 g: 4 mL, subject to ultrasonic extraction at 300 W for 2 h, filter, collect the supernatant, concentrate and dry to obtain clove extract.

[0053] Mix Eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract at a weight ratio of 8:4:4 to obtain a uric acid-lowering composition containing Eucommia ulmoides polysaccharide.

[0054] Example 3:

[0055] The preparation method of Eucommia ulmoides polysaccharide is as follows:

[0056] Dry Eucommia ulmoides leaves and crush them to obtain Eucommia ulmoides leaf powder; mix the Eucommia ulmoides leaf powder and water at a material-liquid ratio of 1 g: 15 mL, subject to ultrasonic treatment at 70 °C and 400 W for 30 min, then centrifuge at 4500 r / min for 8 min, collect the filtrate and concentrate it to obtain a concentrated solution; mix the concentrated solution and absolute ethanol at a volume ratio of 1:2, let it stand for 24 h, filter, collect the precipitate after filtration, perform deproteinization and decolorization for purification and drying to obtain Eucommia ulmoides polysaccharide.

[0057] The preparation method of mulberry leaf extract is as follows:

[0058] Mix the seed liquid of Lactobacillus sakei, the seed liquid of Kluyveromyces marxianus and the seed liquid of Lactobacillus plantarum at a volume ratio of 1: 2.5: 5 to obtain a mixed seed liquid, and adjust the viable count of Lactobacillus sakei in the mixed seed liquid to 1.8×10 6 cfu / mL, the viable count of Kluyveromyces marxianus is 1.8×10 6 cfu / mL, and the viable count of Lactobacillus plantarum is 1.2×10 7 cfu / mL;

[0059] Inoculate the mixed seed liquid into the mulberry leaf liquid medium, wherein the inoculation amount of the mixed seed liquid is 12% of the volume of the mulberry leaf liquid medium, ferment at 37 °C for 7 d, centrifuge to obtain a fermentation broth; concentrate and dry the fermentation broth to obtain mulberry leaf extract;

[0060] The components of the mulberry leaf liquid medium are: 35 mg / L mulberry leaf extract, 220 g / L potato leachate, 25 g / L sucrose; among them, the mulberry leaf extract is prepared by the following method: mix mulberry leaves and water at a material-liquid ratio of 1 g: 25 mL, heat and decoct 2 times, each decocting time is 4 h, combine the decoction liquids, and dry to obtain mulberry leaf extract.

[0061] The preparation method of clove extract is as follows:

[0062] Dry cloves and crush them, mix them with ethanol with a mass fraction of 90% at a material-liquid ratio of 1 g: 6 mL, subject to ultrasonic extraction at 400 W for 3 h, filter, collect the supernatant, concentrate and dry to obtain clove extract.

[0063] Mix Eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract at a weight ratio of 2: 6: 6 to obtain a uric acid-lowering composition containing Eucommia ulmoides polysaccharide.

[0064] Comparative Example 1:

[0065] The difference between this comparative example and Example 1 is that the uric acid-lowering composition consists only of Eucommia ulmoides polysaccharide.

[0066] Comparative Example 2:

[0067] The difference between this comparative example and Example 1 is that the uric acid-lowering composition is made by mixing eucommia ulmoides polysaccharide and mulberry leaf extract in a weight ratio of 10:5.

[0068] Comparative Example 3:

[0069] The difference between this comparative example and Example 1 is that the uric acid-lowering composition is made only of eucommia ulmoides polysaccharide and clove extract in a weight ratio of 10:5.

[0070] Test Example 1:

[0071] Referring to the animal experiment in the reference journal "Matrix Study on the Uric Acid-Lowering Effect of Mulberry Twig Polysaccharide on Hyperuricemic Rats with Kidney Injury" (Zhou Dan et al., West China Journal of Pharmaceutical Sciences, 2023, 38(6): 651-654), the uric acid-lowering effects of the uric acid-lowering compositions prepared in Example 1 and Comparative Examples 1-3 were detected.

[0072] Among them, SPF-grade mice (weight 20±2 g) were selected for the test. Before the test, the SPF mice were adaptively fed for 7 days. The SPF-grade mice were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0073] These 70 SPF mice were randomly divided into 7 groups, namely Treatment Groups 1-7. The details of each treatment group are as follows:

[0074] Treatment Group 1: Blank control group; fed with normal feed and intragastrically administered normal saline daily;

[0075] Treatment Group 2: Hyperuricemia model group; fed with hyperuricemia feed and intragastrically administered normal saline daily;

[0076] Treatment Group 3: Positive control group; fed with hyperuricemia feed and intragastrically administered allopurinol solution daily. The allopurinol solution was made by mixing allopurinol and normal saline, and its concentration was 5 mg / mL;

[0077] Treatment Group 4: Fed with hyperuricemia feed and intragastrically administered uric acid-lowering composition solution daily. The uric acid-lowering composition solution was made by mixing the uric acid-lowering composition prepared in Example 1 and normal saline, and its concentration was 400 mg / kg;

[0078] Treatment Group 5: Fed with hyperuricemia feed and intragastrically administered uric acid-lowering composition solution daily. The uric acid-lowering composition solution was made by mixing the uric acid-lowering composition prepared in Comparative Example 1 and normal saline, and its concentration was 400 mg / kg;

[0079] Treatment Group 6: Fed with hyperuricemia feed and intragastrically administered uric acid-lowering composition solution daily. The uric acid-lowering composition solution was made by mixing the uric acid-lowering composition prepared in Comparative Example 2 and normal saline, and its concentration was 400 mg / kg;

[0080] Treatment group 7: Fed with high-uric acid diet and gavaged with uric acid-lowering composition solution daily. The uric acid-lowering composition solution was prepared by mixing the uric acid-lowering composition obtained in Comparative Example 3 with normal saline, and its concentration was 400 mg / kg;

[0081] During the experiment, the daily feed intake and feed intake time of each group of mice were kept consistent. Among them, the normal feed was purchased from Jinan Pengyue Laboratory Animal Breeding Co., Ltd., and the product name was standard mouse feed; the high-uric acid feed was prepared by mixing normal feed, adenine and potassium oxonate in a mass ratio of 40:0.1:1. Ensure that the daily gavage volume of mice is 0.1 mL / 10 g (body weight), and continuous gavage is carried out for 6 weeks. Each group of mice had a normal diet.

[0082] After the last administration, after anesthesia with isoflurane, blood was taken from the eyeballs. After the blood was naturally coagulated at room temperature for 1 h, it was then centrifuged at 4°C and 3600 r / min for 10 min to obtain serum. The uric acid content in the serum was measured using a biochemical analyzer, and the results are shown in Table 1.

[0083] Table 1 Serum uric acid content in each treatment group

[0084]

[0085] As can be seen from Table 1, when only eucommia ulmoides polysaccharide was gavaged, the serum uric acid content was 85.6 μmol / L. After gavaging eucommia ulmoides polysaccharide and mulberry leaf extract, the serum uric acid content was 80.3 μmol / L. After gavaging eucommia ulmoides polysaccharide and clove extract, the serum uric acid content was 81.6 μmol / L. However, for the uric acid-lowering composition prepared by gavaging eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract in the present invention, the serum uric acid content was 75.2 μmol / L. Thus, it can be seen that eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract have a synergistic effect in reducing the serum uric acid content.

[0086] The above are only the preferred embodiments of the present application and are not used to limit the present application. For those skilled in the art, the present application can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.

Claims

1. A uric acid-lowering composition containing eucommia ulmoides polysaccharide, characterized in that, The uric acid-lowering composition is prepared from eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract according to the weight ratio of (8 - 12):(4 - 6):(4 - 6).

2. The uric acid-lowering composition containing eucommia ulmoides polysaccharide according to claim 1, characterized in that The eucommia ulmoides polysaccharide is prepared by the following method: After mixing eucommia ulmoides leaf powder and water according to the solid-liquid ratio of 1 g:(5 - 15) mL, performing ultrasonic treatment and centrifugation, collecting the filtrate and concentrating it to obtain a concentrated solution; after mixing the concentrated solution and absolute ethanol according to the volume ratio of 1:(2 - 5), standing and then filtering, collecting the precipitate, and performing purification and drying to obtain eucommia ulmoides polysaccharide; During the ultrasonic treatment process, the ultrasonic temperature is 50 - 70 °C, the ultrasonic power is 200 - 400 W, and the ultrasonic time is 0.5 - 1 h.

3. The uric acid-lowering composition containing eucommia ulmoides polysaccharide according to claim 1, wherein, The mulberry leaf extract is prepared by the following method: After mixing the seed liquid of Lactobacillus sakei, the seed liquid of Kluyveromyces marxianus and the seed liquid of Lactobacillus plantarum according to the volume ratio of 1:(1.5 - 2.5):(4 - 5), obtaining a mixed seed liquid; inoculating the mixed seed liquid into a mulberry leaf liquid medium, performing fermentation culture and centrifugation to obtain a fermentation broth; concentrating and drying the fermentation broth to obtain the mulberry leaf extract.

4. The uric acid-lowering composition containing eucommia ulmoides polysaccharide according to claim 3, characterized in that, The preservation number of Lactobacillus sakei is ACCC 05429, the preservation number of Kluyveromyces marxianus is ACCC 20314, and the preservation number of Lactobacillus plantarum is ACCC 03954.

5. The uric acid-lowering composition containing eucommia ulmoides polysaccharide according to claim 3, characterized in that, In the mixed seed liquid, the viable count of Lactobacillus sakei ≥ 1.8×10 6 cfu / mL, the viable count of Kluyveromyces marxianus ≥ 1.8×10 6 cfu / mL, and the viable count of Lactobacillus plantarum ≥ 1.2×10 7 cfu / mL.

6. The uric acid-lowering composition containing eucommia ulmoides polysaccharide according to claim 3, wherein, The components of the mulberry leaf liquid medium are: (180 - 220) g / L potato leaching solution, (15 - 25) g / L sucrose, (25 - 35) mg / L mulberry leaf extraction solution; The preparation method of the mulberry leaf extraction solution is: after mixing mulberry leaves and water according to the solid-liquid ratio of 1 g:(15 - 25) mL, heating and decocting 2 - 3 times, with the decocting time of each time being 3 - 4 h, combining the decoction liquids and drying to obtain the mulberry leaf extraction solution.

7. The uric acid-lowering composition containing eucommia ulmoides polysaccharide according to claim 3, wherein The inoculation amount of the mixed seed liquid is 8 - 12% of the volume of the fermentation medium; during the fermentation culture process, the temperature is 30 - 37 °C and the fermentation time is 5 - 7 d.

8. The uric acid-lowering composition containing eucommia ulmoides polysaccharide according to claim 1, characterized in that, The clove extract is prepared by the following method: After mixing cloves and ethanol with a mass fraction of 75% - 90% according to the solid-liquid ratio of 1 g:(4 - 6) mL, performing ultrasonic extraction at a power of 300 - 400 W for 2 - 3 h, filtering, collecting the supernatant, and performing concentration and drying to obtain the clove extract.

9. A method for preparing a uric acid-lowering composition containing eucommia ulmoides polysaccharide according to any one of claims 1-8, characterized in that, It includes the following steps: After mixing eucommia ulmoides polysaccharide, mulberry leaf extract and clove extract evenly, the uric acid-lowering composition containing eucommia ulmoides polysaccharide is obtained.

10. Use of the uric acid-lowering composition containing eucommia ulmoides polysaccharide according to any one of claims 1 - 8 in the preparation of a uric acid-lowering product.

Images