Preparation method of hair loss prevention, hair growth and oil control shampoo based on plant active ingredients

Abstract

The invention relates to the field of shampoo preparation, and particularly discloses a preparation method of anti-hair-loss, hair-growth and oil-control shampoo based on plant active ingredients, which comprises the following steps: step 1, soaking folium artemisiae argyi and pepper in warm water of 50-60 DEG C for 30 minutes, decocting for 30 minutes with medium fire, and filtering to obtain a first extracting solution; the preparation method comprises the following steps: soaking soapberry, cacumen biotae, duckweed, fructus cnidii, fructus kochiae, angelica sinensis, radix rehmanniae, radix paeoniae alba, rhizoma atractylodis macrocephalae, coptis chinensis, golden cypress, scutellaria baicalensis, radix sophorae flavescentis and ligusticum wallichii for 2 hours, and decocting at 95-100 DEG C for 4 hours; a functional closed loop of'oil control purification-hair follicle activation-inflammation blocking 'is formed through a three-element synergistic mechanism of sapindus, cacumen biotae and duckweed, sapindus saponin constructs microenvironment balance through targeted removal of hair follicle fat suppository, cacumen biotae flavone activates a hair papilla cell proliferation signal channel, duckweed polysaccharide forms an anti-inflammatory protection barrier, and the anti-inflammatory effect is achieved. The three components form a three-dimensional protective network on the molecular level, so that the pathological process of seborrheic alopecia is effectively blocked.

Description

Technical Field

[0001] The present invention belongs to the field of shampoo preparation, and specifically relates to a method for preparing an anti-hair loss, hair growth-promoting and oil-control shampoo based on plant active ingredients. Background Art

[0002] A shampoo is a personal care product that cleans the scalp and hair through physical and chemical actions, and is used to remove oil, sweat, environmental pollutants and residues of hair care products.

[0003] Traditional commercially available anti-hair loss products mostly rely on a single active ingredient, such as minoxidil and ketoconazole, which only target a single path of hair follicle activation or antibacterial, and cannot block the vicious cycle of "excessive oil → inflammation → hair follicle atrophy", making it difficult to form a cross-level scalp microenvironment governance system. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a method for preparing an anti-hair loss, hair growth-promoting and oil-control shampoo based on plant active ingredients, so as to solve the problem that traditional commercially available anti-hair loss products in the prior art mostly rely on a single active ingredient and cannot block the vicious cycle of "excessive oil → inflammation → hair follicle atrophy".

[0005] The anti-hair loss, hair growth-promoting and oil-control shampoo based on plant active ingredients contains the following active ingredients by weight:

[0006] 12 - 17 parts of Sapindus mukorossi, 7 - 11 parts of Platycladus orientalis, 7 - 11 parts of Lemna minor, 2 - 4 parts of Cnidium monnieri, 2 - 4 parts of Kochia scoparia, 1 - 3 parts of Artemisia argyi, 1 - 3 parts of Zanthoxylum bungeanum, 2 - 4 parts of Angelica sinensis, 2 - 4 parts of Rehmannia glutinosa, 2 - 4 parts of Paeonia lactiflora, 2 - 4 parts of Atractylodes macrocephala, 2 - 4 parts of Coptis chinensis, 2 - 4 parts of Phellodendron amurense, 2 - 4 parts of Scutellaria baicalensis, 2 - 4 parts of Sophora flavescens, 1 - 3 parts of Ligusticum wallichii.

[0007] The method for preparing the anti-hair loss, hair growth-promoting and oil-control shampoo based on plant active ingredients includes the following steps:

[0008] Step 1: Soak Artemisia argyi and Zanthoxylum bungeanum in warm water at 50 - 60 °C for 30 minutes, then decoct with medium heat for 30 minutes, and filter to obtain the first extract;

[0009] Soak Sapindus mukorossi, Platycladus orientalis, Lemna minor, Cnidium monnieri, Kochia scoparia, Angelica sinensis, Rehmannia glutinosa, Paeonia lactiflora, Atractylodes macrocephala, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Sophora flavescens and Ligusticum wallichii for 2 hours, then decoct at 95 - 100 °C for 4 hours, and concentrate under reduced pressure to 20 - 30% of the original volume at 50 - 60 °C, and filter to obtain the second extract;

[0010] Step 2: Mix the first extract and the second extract in a volume ratio of 1:2 - 1:3;

[0011] Step 3: Add surfactant, thickener and preservative, adjust the pH to 5.5 - 6.5, and fill the product after homogenization.

[0012] Preferably, in Step 1, the sapindus, oriental arborvitae leaf, duckweed, fructus cnidii, and fructuskochiae are pretreated as follows: After crushing the medicinal materials to 40 - 60 mesh, add a compound enzyme preparation composed of cellulase and pectinase in a mass ratio of 1:1.5, and perform enzymatic hydrolysis in a buffer solution with a pH of 4.5 - 5.0 at 45°C for 40 minutes. After inactivation, perform soaking.

[0013] Preferably, the addition amount of the compound enzyme preparation is 0.8 - 1.2% of the total weight of the medicinal materials. During the enzymatic hydrolysis process, ultrasonic waves with a frequency of 20 - 30 kHz are applied synchronously, and the power density is 50 - 80 W / L.

[0014] Preferably, after mixing the extraction solutions in Step 2, add β - cyclodextrin embedding agent and vitamin E. The addition amount of β - cyclodextrin is 0.5 - 1.0% of the weight of the mixed solution, and the addition amount of vitamin E is 0.05 - 0.1%.

[0015] The β - cyclodextrin is modified by hydroxypropyl, and the weight embedding ratio with the active ingredient is 1:20 - 1:30.

[0016] Preferably, in Step 3, the surfactant is a composite system prepared by compounding sodium lauroyl sarcosinate and cocamidopropyl betaine in a ratio of 3:1, and the total addition amount is 10 - 12%.

[0017] Preferably, the homogenization treatment is carried out using a high - pressure micro - jet homogenizer, controlling the pressure at 150 - 180 MPa and performing cyclic treatment 3 - 5 times.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] Through the ternary synergistic mechanism of sapindus - oriental arborvitae leaf - duckweed, a functional closed - loop of "oil control and purification - hair follicle activation - inflammation block" is formed. Among them, sapindus saponin constructs a micro - environmental balance by targeting and removing hair follicle fat plugs, oriental arborvitae leaf flavonoids activate the proliferation signal pathway of dermal papilla cells, and duckweed polysaccharides form an anti - inflammatory protection barrier. The three form a three - dimensional protection network at the molecular level, effectively blocking the pathological process of seborrheic alopecia.

[0020] Through the innovative application of the compound enzymatic hydrolysis - ultrasonic wave synergistic extraction technology, the bioavailability of active ingredients is significantly improved.

[0021] Angelica sinensis, rehmannia glutinosa, and paeonia lactiflora inhibit the inflammatory response by regulating the scalp immune micro - environment; the alkaloid components of coptis chinensis, phellodendron amurense, and scutellaria baicalensis synergistically inhibit the growth of fungi and bacteria; atractylodes macrocephala balances sebum secretion, ligusticum wallichii promotes scalp microcirculation, and sophora flavescens inhibits androgen metabolism. Multiple targets synergistically consolidate the core efficacy. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the preparation method of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0024] As Figure 1 shown

[0025] Example 1

[0026] Formulation composition (parts by weight):

[0027] 12 parts of Sapindus mukorossi, 7 parts of Platycladus orientalis, 7 parts of Lemna minor, 2 parts of Cnidium monnieri, 2 parts of Kochia scoparia, 1 part of Artemisia argyi, 1 part of Zanthoxylum bungeanum, 2 parts of Angelica sinensis, 2 parts of Rehmannia glutinosa, 2 parts of Paeonia lactiflora, 2 parts of Atractylodes macrocephala, 2 parts of Coptis chinensis, 2 parts of Phellodendron amurense, 2 parts of Scutellaria baicalensis, 2 parts of Sophora flavescens, 1 part of Ligusticum chuanxiong

[0028] Preparation method:

[0029] First extraction solution:

[0030] 1 part of Artemisia argyi and 1 part of Zanthoxylum bungeanum are soaked in warm water at 50°C for 30 minutes, decocted over medium heat for 30 minutes, and filtered.

[0031] Pretreatment of the second extraction solution:

[0032] Sapindus mukorossi, Platycladus orientalis, Lemna minor, Cnidium monnieri, Kochia scoparia, Angelica sinensis, Rehmannia glutinosa, Paeonia lactiflora, Atractylodes macrocephala, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Sophora flavescens and Ligusticum chuanxiong are pulverized to 40 meshes, 0.8% complex enzyme (cellulase: pectinase = 1:1.5) is added, and in a buffer solution with pH 4.5, enzymatic hydrolysis is carried out at 45°C for 40 minutes (simultaneously applying ultrasonic wave at 20 kHz, 50 W / L), and inactivated at 90°C.

[0033] After soaking, decoct at 95°C for 4 hours, concentrate under reduced pressure at 50°C to 20% of the original volume, and filter.

[0034] Mixing and compounding:

[0035] The first and second extraction solutions are mixed in a ratio of 1:2, and 0.5% hydroxypropyl-β-cyclodextrin (inclusion ratio 1:20) and 0.05% vitamin E are added.

[0036] Add 10% surfactant (sodium lauroyl sarcosinate: betaine = 3:1), adjust the pH to 5.5, and fill after homogenizing 3 times with high-pressure microfluidization at 150 MPa.

[0037] Example 2

[0038] Formulation composition (parts by weight):

[0039] 14.5 parts of Sapindus mukorossi, 9 parts of Platycladus orientalis leaves, 9 parts of Lemna minor, 3 parts of Cnidium monnieri, 3 parts of Kochia scoparia, 2 parts of Artemisia argyi, 2 parts of Zanthoxylum bungeanum, 3 parts of Angelica sinensis, 3 parts of Rehmannia glutinosa, 3 parts of Paeonia lactiflora, 3 parts of Atractylodes macrocephala, 3 parts of Coptis chinensis, 3 parts of Phellodendron amurense, 3 parts of Scutellaria baicalensis, 3 parts of Sophora flavescens, 2 parts of Ligusticum wallichii

[0040] Preparation method:

[0041] First extract:

[0042] 2 parts of Artemisia argyi and 2 parts of Zanthoxylum bungeanum are soaked in warm water at 55 °C for 30 minutes, decocted over medium heat for 30 minutes, and filtered.

[0043] Pretreatment of the second extract:

[0044] Sapindus mukorossi, Platycladus orientalis leaves, Lemna minor, Cnidium monnieri, Kochia scoparia, Angelica sinensis, Rehmannia glutinosa, Paeonia lactiflora, Atractylodes macrocephala, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Sophora flavescens and Ligusticum wallichii are pulverized to 50 mesh, 1.0% complex enzyme (cellulase: pectinase = 1:1.5) is added, and enzymolysis is carried out at 45 °C for 40 minutes (synchronously applying 25 kHz ultrasonic wave, 65 W / L) in a pH 4.75 buffer solution, and inactivated at 87.5 °C.

[0045] After soaking, decoct at 97.5 °C for 4 hours, concentrate under reduced pressure at 55 °C to 25% of the original solution volume, and filter.

[0046] Mixing and compounding:

[0047] The first and second extracts are mixed in a ratio of 1:2.5, and 0.75% hydroxypropyl-β-cyclodextrin (inclusion ratio 1:25) and 0.075% vitamin E are added.

[0048] Add 11% surfactant (sodium lauroyl sarcosinate: betaine = 3:1), adjust the pH to 6.0, and fill after homogenizing 4 times with high-pressure microfluidization at 165 MPa.

[0049] Example 3

[0050] Formulation composition (parts by weight):

[0051] 17 parts of Sapindus mukorossi, 11 parts of Platycladus orientalis leaves, 11 parts of Lemna minor, 4 parts of Cnidium monnieri, 4 parts of Kochia scoparia, 3 parts of Artemisia argyi, 3 parts of Zanthoxylum bungeanum, 4 parts of Angelica sinensis, 4 parts of Rehmannia glutinosa, 4 parts of Paeonia lactiflora, 4 parts of Atractylodes macrocephala, 4 parts of Coptis chinensis, 4 parts of Phellodendron amurense, 4 parts of Scutellaria baicalensis, 4 parts of Sophora flavescens, 3 parts of Ligusticum wallichii

[0052] Preparation method:

[0053] First extract:

[0054] Soak 3 parts of Artemisia argyi and 3 parts of Chinese prickly ash in warm water at 60°C for 30 minutes, decoct with medium heat for 30 minutes, and filter.

[0055] Pretreatment of the second extract:

[0056] Sapindus mukorossi, Platycladus orientalis, Lemna minor, Cnidium monnieri, Kochia scoparia, Angelica sinensis, Rehmannia glutinosa, Paeonia lactiflora, Atractylodes macrocephala, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Sophora flavescens and Ligusticum chuanxiong are crushed to 60 meshes, 1.2% complex enzyme (cellulase: pectinase = 1:1.5) is added, and in a buffer solution with pH 5.0, enzymolysis is carried out at 45°C for 40 minutes (synchronously applying ultrasonic wave at 30 kHz, 80 W / L), and inactivated at 85°C.

[0057] After soaking, decoct at 100°C for 4 hours, concentrate under reduced pressure at 60°C to 30% of the original volume, and filter.

[0058] Mixing and compounding:

[0059] Mix the first and second extracts in a ratio of 1:3, add 1.0% hydroxypropyl β-cyclodextrin (inclusion ratio 1:30) and 0.1% vitamin E.

[0060] Add 12% surfactant (sodium lauroyl sarcosinate: betaine = 3:1), adjust the pH to 6.5, and fill after homogenizing 5 times by high-pressure microfluidization at 180 MPa.

[0061] Experimental example 1:

[0062] Experimental purpose: To verify the irreplaceability of Sapindus mukorossi, Platycladus orientalis, Lemna minor and their synergistic process in the effects of preventing hair loss, promoting hair growth and controlling oil in Example 2.

[0063] I. Experimental scheme

[0064] Experimental grouping (30 people in each group, patients with seborrheic alopecia, experimental period 12 weeks):

[0065] Example 2 group: Use the shampoo prepared in Example 2 (containing all ingredients and composite process); Comparative example 1: Remove Sapindus mukorossi, and keep the rest of the ingredients and process unchanged;

[0066] Comparative example 2: Remove Platycladus orientalis, and keep the rest of the ingredients and process unchanged;

[0067] Comparative example 3: Remove Lemna minor, and keep the rest of the ingredients and process unchanged;

[0068] Comparative example 4: Remove Sapindus mukorossi, Platycladus orientalis and Lemna minor at the same time, and only keep other ingredients;

[0069] Blank control group: Commercially available ordinary oil-control shampoo (containing zinc pyrithione).

[0070] Detection indicators:

[0071] Oil-control effect: Scalp sebum secretion amount ( measurement);

[0072] Anti-hair loss effect: Daily hair loss count;

[0073] Hair growth effect: Hair follicle density ( image analysis);

[0074] Component permeability: Fluorescent labeling concentration of key active components (flavonoids, saponins) in hair follicles.

[0075] II. Experimental data table

[0076]

[0077]

[0078] III. Analysis of experimental results

[0079] Irreplaceability of core components:

[0080] Absence of Sapindus mukorossi (comparative example 1): The sebum reduction rate decreased by 38.8%, and the saponin retention rate decreased by 23.7%, proving that Sapindus mukorossi saponin is the core oil-control component, and its surfactant effect can deeply clean the lipid in hair follicles;

[0081] Absence of Platycladus orientalis (comparative example 2): The hair follicle density increase rate decreased by 69%, and the flavonoid permeability was 0, indicating that Platycladus orientalis flavonoids directly promote hair follicle regeneration by activating the Wnt / β-catenin pathway;

[0082] Absence of Lemna minor (comparative example 3): The hair loss reduction rate decreased by 22.7%, because Lemna minor polysaccharide can inhibit the IL-6 inflammatory factor and block the transformation of hair follicles into the telogen phase.

[0083] Synergistic mechanism:

[0084] The hair follicle density increase rate of the group in Example 2 (31.5 / cm 2 ) was significantly higher than that of each single-component absence group, verifying the synergistic logic of "Sapindus mukorossi controls oil → Platycladus orientalis promotes hair growth → Lemna minor reduces inflammation";

[0085] The anti-hair loss effect of comparative example 4 (absence of all components) was close to that of the blank control group, indicating that other components (such as Coptis chinensis and Sophora flavescens) only play an auxiliary antibacterial role and cannot replace the functions of the core three components.

[0086] Effect of process on component stability:

[0087] The saponin retention rate of the Example 2 group (93.7%) was much higher than that of Comparative Examples 1-4, proving that the combined enzymatic hydrolysis-ultrasonic process can reduce the damage to the saponin structure caused by high temperature;

[0088] Since the blank control group does not contain plant active ingredients, it has no competitiveness in terms of permeability and stability indicators.

[0089] IV. Conclusion

[0090] Through the Sapindus-Platycladus orientalis-Lemna minor ternary synergistic system and the directional extraction process of the present invention, significant effects of oil control (sebum reduction rate ≥ 68%), anti-hair loss (hair loss reduction rate ≥ 74%), and hair growth (hair follicle density increase ≥ 31 per cm 2 ) are achieved, and the data stability (ingredient retention rate ≥ 93%) is superior to that of traditional formulations.

[0091] Experimental Example 2:

[0092] Experimental purpose: To verify the effects of combined enzymatic hydrolysis, ultrasonic assistance, and the application of embedding agent in Example 2 on the retention rate of active ingredients in shampoo and the effects of oil control and anti-hair loss.

[0093] I. Experimental scheme

[0094] Setting of comparative examples (each experiment is repeated 3 times):

[0095] Comparative Example 1: Cancel the steps of combined enzymatic hydrolysis and ultrasonic treatment, and only extract the medicinal materials by conventional decoction method;

[0096] Comparative Example 2: Use a single enzyme (only cellulase) to replace the combined enzyme, and other processes are the same as in Example 2;

[0097] Comparative Example 3: Cancel hydroxypropyl-β-cyclodextrin and vitamin E, and directly mix the extraction solution;

[0098] Comparative Example 4: Change the surfactant to a single component (only sodium lauroyl sarcosinate), and the total addition amount remains unchanged;

[0099] Comparative Example 5: Reduce the homogenization pressure to 100 MPa, and the number of cycles is 1 time.

[0100] Detection indicators:

[0101] Content of active ingredients: HPLC determination of flavonoids (Platycladus orientalis), saponins (Sapindus), and alkaloids (Coptis chinensis);

[0102] Oil control effect: Inhibitory rate of sebum secretion by in vitro sebaceous gland cells (SZ95);

[0103] Anti-hair loss effect: Proliferation rate of human dermal papilla cells (DPC) (MTT method);

[0104] Stability: Retention rate of active ingredients after 30 days of storage;

[0105] Physical properties: foam volume (GB / T 13173), viscosity (rotational viscometer).

[0106] II. Experimental data table

[0107]

[0108]

[0109] III. Analysis of experimental results

[0110] Synergistic effect of enzymatic hydrolysis and ultrasonic wave:

[0111] The content of active ingredients (flavonoids, saponins) in Comparative Example 1 (without enzymatic hydrolysis / ultrasonic wave) decreased by 40 - 45%, proving that the combined enzymatic hydrolysis and ultrasonic wave can significantly improve the cell wall breaking rate and promote the dissolution of components.

[0112] The saponin extraction rate in Comparative Example 2 (single enzyme) was 19% lower than that in Example 2, indicating that the compound of cellulase and pectinase can specifically decompose the cellulose-pectin composite structure in the medicinal material cell wall.

[0113] Effect of embedding agent on stability:

[0114] The alkaloid retention rate in Comparative Example 3 (without β-cyclodextrin) decreased by 20.1%, and the component loss was serious after storage (the retention rate was only 63.8%), indicating that hydroxypropyl β-cyclodextrin can effectively prevent oxidation and hydrolysis through the embedding effect.

[0115] The addition of vitamin E further inhibits the degradation of fat-soluble components (such as tetramethylpyrazine) and improves the anti-hair loss effect.

[0116] Cleanliness and mildness of surfactant compounding:

[0117] The foam volume in Comparative Example 4 (single surfactant) decreased by 8%, and the oil control rate decreased by 6%, indicating that the 3:1 compound of sodium lauroyl sarcosinate and betaine has both degreasing power and mildness, avoiding excessive cleaning and damaging the scalp barrier.

[0118] Optimization of physical properties by high-pressure homogenization:

[0119] The viscosity in Comparative Example 5 (low-pressure homogenization) decreased by 1.5%, and the foam stability decreased, indicating that high-pressure treatment at 150 - 180 MPa can refine particles (particle size ≤ 200 nm) and improve the sensory quality and use experience of shampoo.

[0120] IV. Experimental conclusion

[0121] Example 2 achieved the efficient extraction and long-term stability of active ingredients through the combined enzymatic hydrolysis-ultrasonic synergistic extraction, β-cyclodextrin embedding stabilization, and surfactant compounding process, and its oil control and anti-hair loss effects were significantly better than those of the conventional process (Comparative Examples 1-5).

[0122] Experimental Example 3: Verify the therapeutic effects of auxiliary ingredients (Angelica sinensis, Rehmannia glutinosa, etc.) on folliculitis and seborrheic alopecia

[0123] I. Experimental scheme

[0124] Experimental purpose: Verify the synergistic therapeutic effects of auxiliary ingredients such as Angelica sinensis, Rehmannia glutinosa, Paeonia lactiflora, Atractylodes macrocephala, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Sophora flavescens, and Ligusticum chuanxiong on folliculitis and seborrheic alopecia.

[0125] Experimental grouping (30 patients with seborrheic alopecia complicated with folliculitis in each group, experimental period: 12 weeks):

[0126] Example 2 group: Use the shampoo prepared in Example 2 (containing all ingredients and compound process);

[0127] Comparative Example A: Remove Angelica sinensis, Rehmannia glutinosa, and Paeonia lactiflora, and keep the other ingredients and process unchanged;

[0128] Comparative Example B: Remove Atractylodes macrocephala, Coptis chinensis, and Phellodendron amurense, and keep the other ingredients and process unchanged;

[0129] Comparative Example C: Remove Scutellaria baicalensis, Sophora flavescens, and Ligusticum chuanxiong, and keep the other ingredients and process unchanged;

[0130] Comparative Example D: Only retain the core three ingredients (Sapindus mukorossi, Platycladus orientalis, and Lemna minor), and remove other auxiliary ingredients;

[0131] Blank control group: Commercially available oil-control shampoo containing zinc pyrithione.

[0132] Detection indicators:

[0133] Improvement of folliculitis: Concentrations of scalp inflammatory factors IL-6 and TNF-α (ELISA method);

[0134] Oil control effect: Sebum secretion amount

[0135] Anti-hair loss effect: Daily hair loss amount;

[0136] Hair growth effect: Hair follicle density

[0137] Antibacterial effect: Malassezia inhibition rate (in vitro culture method).

[0138] II. Experimental data table

[0139]

[0140]

[0141] III. Analysis of Experimental Results

[0142] Verification of anti-inflammatory effect:

[0143] Control Example A (removing Angelica sinensis, Rehmannia glutinosa, Paeonia lactiflora): The reduction rates of IL-6 and TNF-α decreased by 19.6% and 22.9% respectively, indicating that Angelica sinensis, Rehmannia glutinosa, and Paeonia lactiflora synergistically inhibit inflammation by regulating the immune response.

[0144] Control Example B (removing Atractylodes macrocephala, Coptis chinensis, Phellodendron amurense): The reduction rate of IL-6 decreased by 37.0%. Because the alkaloids in Coptis chinensis and Phellodendron amurense directly inhibit pathogenic bacteria, and Atractylodes macrocephala regulates sebum secretion to block the inducement of inflammation.

[0145] Control Example D (only retaining the core three components): The reduction rate of inflammatory factors was significantly lower than that of Example 2 group (IL-6 decreased by 46.8%), proving that the auxiliary components are indispensable for the improvement of folliculitis.

[0146] Synergy between oil control and anti-hair loss:

[0147] Control Example C (removing Scutellaria baicalensis, Sophora flavescens, Ligusticum chuanxiong): The reduction rate of hair loss decreased by 14.3%. Because baicalin and matrine can inhibit the activity of 5α-reductase, reduce the production of DHT, and Ligusticum chuanxiong promotes scalp microcirculation. The three of them jointly delay follicular atrophy.

[0148] Antibacterial effect:

[0149] Control Example B (removing Coptis chinensis, Phellodendron amurense, Atractylodes macrocephala): The inhibition rate of Malassezia decreased by 23.7%, proving that the alkaloids of Coptis chinensis and Phellodendron amurense have a strong inhibitory effect on fungi, and Atractylodes macrocephala indirectly inhibits fungal reproduction by controlling oil.

[0150] Comprehensive efficacy:

[0151] The follicle density in Example 2 group increased (31.5 follicles / cm 2 ) significantly higher than all control examples, indicating that the auxiliary components synergistically enhance the anti-hair loss and hair growth effect of the core three components through multiple targets (anti-inflammatory, antibacterial, promoting blood circulation).

[0152] IV. Experimental Conclusions

[0153] Auxiliary components such as Angelica sinensis, Rehmannia glutinosa, Paeonia lactiflora, Atractylodes macrocephala, Coptis chinensis, Phellodendron amurense, Scutellaria baicalensis, Sophora flavescens, and Ligusticum chuanxiong enhance the therapeutic effect of shampoo on folliculitis and seborrheic alopecia through the following mechanisms:

[0154] Anti-inflammatory: inhibiting the release of inflammatory factors such as IL-6 and TNF-α (Angelica sinensis, Scutellaria baicalensis);

[0155] Antibacterial: directly killing Malassezia (Coptis chinensis, Phellodendron amurense);

[0156] Regulate microcirculation: Promote the nutrient supply to hair follicles (Chuanxiong Rhizoma, Rehmanniae Radix).

[0157] Synergistically control oil: Reduce the generation of DHT (Sophora flavescens Ait., Atractylodes macrocephala Koidz.).

[0158] Removing any auxiliary component results in a significant decrease in key indicators, verifying its necessity in the "oil control - anti - inflammation - hair growth" functional chain.

[0159] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above - mentioned terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0160] In the attached drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are involved. Other structures can refer to the general design. Without conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0161] Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An anti - hair loss, hair growth - promoting and oil - controlling shampoo based on plant active ingredients, characterized in that, Comprising the following active ingredients by weight parts: Sapindus mukorossi Gaertn. 12 - 17 parts, Platycladus orientalis (L.) Franco 7 - 11 parts, Lemna minor L. 7 - 11 parts, Cnidium monnieri (L.) Cuss. 2 - 4 parts, Kochia scoparia (L.) Schrad. 2 - 4 parts, Artemisia argyi Levl. et Vant. 1 - 3 parts, Zanthoxylum bungeanum Maxim. 1 - 3 parts, Angelica sinensis (Oliv.) Diels 2 - 4 parts, Rehmannia glutinosa Libosch. 2 - 4 parts, Paeonia lactiflora Pall. 2 - 4 parts, Atractylodes macrocephala Koidz. 2 - 4 parts, Coptis chinensis Franch. 2 - 4 parts, Phellodendron amurense Rupr. 2 - 4 parts, Scutellaria baicalensis Georgi 2 - 4 parts, Sophora flavescens Ait. 2 - 4 parts, Ligusticum wallichii Franch. 1 - 3 parts.

2. Preparation method of anti - hair - loss, hair - growth promoting and oil - control shampoo based on plant active ingredients, characterized in that, Including the following steps: Step 1: Soak Artemisia argyi Levl. et Vant. and Zanthoxylum bungeanum Maxim. in warm water at 50 - 60 °C for 30 minutes, decoct with medium heat for 30 minutes, and filter to obtain the first extract; After soaking Sapindus mukorossi Gaertn., Platycladus orientalis (L.) Franco, Lemna minor L., Cnidium monnieri (L.) Cuss., Kochia scoparia (L.) Schrad., Angelica sinensis (Oliv.) Diels, Rehmannia glutinosa Libosch., Paeonia lactiflora Pall., Atractylodes macrocephala Koidz., Coptis chinensis Franch., Phellodendron amurense Rupr., Scutellaria baicalensis Georgi, Sophora flavescens Ait. and Ligusticum wallichii Franch. for 2 hours, decoct at 95 - 100 °C for 4 hours, concentrate under reduced pressure to 20 - 30% of the original volume at 50 - 60 °C, and filter to obtain the second extract; Step 2: Mix the first extract and the second extract at a volume ratio of 1:2 - 1:3; Step 3: Add a surfactant, a thickener and a preservative, adjust the pH to 5.5 - 6.5, and fill after homogenization.

3. The preparation method of the anti - hair - loss, hair - growth promoting and oil - control shampoo based on plant active ingredients according to claim 2, wherein, In Step 1, the pretreatment of Sapindus mukorossi Gaertn., Platycladus orientalis (L.) Franco, Lemna minor L., Cnidium monnieri (L.) Cuss. and Kochia scoparia (L.) Schrad. is as follows: After crushing the medicinal materials to 40 - 60 meshes, add a composite enzyme preparation composed of cellulase and pectinase at a mass ratio of 1:1.5, in a buffer solution with pH 4.5 - 5.0, carry out enzymatic hydrolysis at 45 °C for 40 minutes, inactivate and then soak.

4. The preparation method of the anti - hair - loss, hair - growth promoting and oil - controlling shampoo based on plant active ingredients according to claim 3, characterized in that, The addition amount of the composite enzyme preparation is 0.8 - 1.2% of the total weight of the medicinal materials, and ultrasonic waves of 20 - 30 kHz are applied synchronously during the enzymatic hydrolysis process, with a power density of 50 - 80 W / L.

5. The preparation method of the anti - hair - loss, hair - growth promoting and oil - controlling shampoo based on plant active ingredients according to claim 2, characterized in that, After mixing the extraction liquids in Step 2, add β - cyclodextrin embedding agent and vitamin E, wherein the addition amount of β - cyclodextrin is 0.5 - 1.0% of the weight of the mixed liquid, and the addition amount of vitamin E is 0.05 - 0.1%; The β - cyclodextrin is modified by hydroxypropyl group, and the weight embedding ratio with the active ingredient is 1:20 - 1:

30.

6. The preparation method of the anti - hair - loss, hair - growth - promoting and oil - controlling shampoo based on plant active ingredients according to claim 2, wherein, In Step 3, the surfactant is a composite system prepared by compounding sodium lauroyl sarcosinate and cocamidopropyl betaine at a ratio of 3:1, and the total addition amount is 10 - 12%.

7. The preparation method of the anti - hair - loss, hair - growth promoting and oil - controlling shampoo based on plant active ingredients according to claim 2, wherein, The homogenization treatment is carried out by a high - pressure microfluidic homogenizer, controlling the pressure at 150 - 180 MPa and circulating for 3 - 5 times.

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