Salt-tolerant and phosphorus-solubilizing penicillium oxalicum and products and applications thereof

Abstract

The application belongs to the technical field of microorganisms, and particularly relates to a salt stress-resistant and phosphorus-solubilizing Penicillium oxalicum as well as products and applications thereof. The application provides the Penicillium oxalicum, which is preserved on October 26, 2022, preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 40346. The Penicillium oxalicum provided by the application has the functions of salt stress resistance and phosphorus solubilization, can improve the utilization rate of soil insoluble phosphate in a saline-alkali soil, significantly improves the content of soluble phosphorus in the soil, has a wide application prospect in soil remediation and soil fertility improvement in the saline-alkali soil, and has far-reaching significance in agricultural production activities.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to a salt-resistant, phosphorus-solubilizing Penicillium oxalate, its products, and applications. Background Technology

[0002] Phosphorus is an essential element for life, a crucial component of DNA, and the phosphorus (P) in ATP (adenosine triphosphate, the molecule that provides energy to cells). Plants need phosphorus to grow, hence the application of phosphate fertilizers to crops. Soluble phosphorus plays a vital role in plant growth, but the high salt concentration stress has led to poor soil quality in the Yellow River Delta saline-alkali land, resulting in low soluble phosphorus content and low soil microbial activity.

[0003] Phosphorus solubilization, also known as phosphorus removal, refers to the process by which organic phosphorus compounds in soil are converted into phosphates (POT) or insoluble phosphorus is converted into soluble phosphorus under the action of microorganisms. This increases the content of available phosphorus in the soil, which is beneficial to plant growth. By screening for microorganisms with phosphorus-solubilizing properties, the conversion of organic phosphorus compounds in soil into phosphates or the conversion of insoluble phosphorus into soluble phosphorus can be promoted.

[0004] Chinese patent application 202210830139.3 discloses a phosphate-solubilizing bacterium, species name: Pantotheca ( ). Pantoea sp. GRINML12, deposited at the China Center for Type Culture Collection, Wuhan University, Wuhan, China, on November 20, 2019, with accession number CCTCC NO: M2019960, utilizes this bacterium to dissolve insoluble inorganic phosphorus sources as free phosphates. In the uranium-contaminated system, U(VI) in the free phosphate undergoes a co-precipitation reaction with the uranium phosphate to form stable uranyl phosphate minerals.

[0005] Chinese patent application 202310835034.1 discloses a type of Klebsiella variegata M15C3 and its applications. This Klebsiella variegata M15C3 possesses phosphorus-solubilizing ability, which can increase the content of available phosphorus in the soil and improve the soil environment. Klebsiella variegata M15C3 plays a positive role in accelerating the conversion of straw returned to the field, improving soil fertility, and promoting crop growth.

[0006] Penicillium oxalate ( Penicillium oxalicum ) is a fungus that is widely found in nature and belongs to the genus Penicillium. Penicillium). It has important application value in agriculture, industry and environment, especially in phosphorus solubilization, biological control and biodegradation. The mechanism of Penicillium oxalicum in phosphorus solubilization mainly depends on the organic acids produced by it. These organic acids can reduce the pH value of the soil, thereby increasing the solubility of the insoluble inorganic phosphorus. At the same time, the organic acids can also combine with metal ions in the soil to form soluble metal-organic acid complexes, further promoting the release of phosphorus. In addition, Penicillium oxalicum also secretes phosphatase (such as acid phosphatase) to decompose organic phosphorus compounds and release inorganic phosphorus that can be absorbed by plants. Specifically, Penicillium oxalicum produces various organic acids such as oxalic acid, citric acid, malic acid, etc. during growth. These organic acids can convert insoluble phosphorus compounds in the soil into soluble phosphorus through dissolution. In addition, Penicillium oxalicum can also adjust the soil environment, such as improving the aeration and water permeability of the soil, which is conducive to the growth and development of plant roots, thereby indirectly promoting the absorption and utilization of phosphorus by plants.

[0007] As a multifunctional fungus, Penicillium oxalicum has important application value in phosphorus solubilization, biodegradation and industrial production. Therefore, it is urgent to develop more efficient and environmentally friendly phosphorus-solubilizing Penicillium oxalicum.

[0008] The saline-alkali soil has high salt content and poor soil, which makes it difficult for plants and microorganisms to survive. Even if there are salt-tolerant plants that can grow, they lack nutrients and grow slowly. Artificial addition of P fertilizer is costly, and it is easy to lose and has low utilization rate. Therefore, it is a difficult problem to be solved to use microorganisms that can tolerate salt stress and solubilize phosphorus to improve the soil of saline-alkali land. SUMMARY

[0009] In order to overcome the defects of the prior art, the present application screens for phosphorus-solubilizing and growth-promoting bacteria in the rhizosphere soil of a pioneer plant for ecological restoration of saline-alkali land, and finally obtains a Penicillium oxalicum (P. oxalicum) strain. Penicillium oxalicum ).

[0010] The technical solution of the present application to achieve the above technical purpose is as follows:

[0011] On the one hand, the present application provides a Penicillium oxalicum, which was deposited on October 26, 2022 at the China General Microbiological Culture Collection Center, and has a deposit number of CGMCC No. 40346.

[0012] On the other hand, the present application provides a culture, which is obtained by inoculating the aforementioned Penicillium oxalicum in a culture medium.

[0013] The inoculation amount of the inoculation can be 0.1-20%, in some cases, it can also be a higher or lower inoculation amount. Specifically, the inoculation amount can be 1-20%, 2-20%, 1-15%, 1-10%, 1-5%, 1-8%, 5-15%, 5-10%, 5-8%, 8-10%, 8-15%, 5-12%, 2-7%.

[0014] Specifically, the culture medium can be a solid culture medium, a semi-solid culture medium or a liquid culture medium, more specifically, it can be any suitable culture medium type disclosed in the prior art, it can also be a culture medium further improved on the culture medium type disclosed in the prior art to improve the performance of the strain, and it can also be a culture medium not disclosed in the prior art but capable of being used for the culture of the aforementioned Penicillium oxalicum.

[0015] The culture conditions of the Penicillium oxalicum provided by the present application include temperature, oxygen content, in some cases, it can also include light, carbon dioxide content, pH, humidity, stirring condition, ventilation condition, oscillation condition, medium replacement condition, culture days, resistance condition, etc.

[0016] Preferably, the temperature in the culture condition can be 20-30℃, specifically, it can be 25-30℃, 26-30℃, 2-30℃, 28-30℃, 29-30℃, 21-30℃, 22-30℃, 23-30℃, 24-30℃, 21-29℃, 21-28℃, 21-27℃, 21-26℃, 21-25℃, 29.5-30℃, 25.5-29.5℃, 27-29.5℃ or 26-27.5℃. Further preferably, it is 29.5-30℃, and more further, it is 30℃.

[0017] Preferably, the culture is an anaerobic culture, and the oxygen content in the culture condition can be 0-8%, specifically, it can be 0-7%, 0-6%, 0-5%, 0-4%, 0-3%, 0-2%, 0-1%, 0-5.5%, 0-3.5%, 0-2.5%, 0-0.5%, 1-8%, 1-5%, 1-2% or 0.5-2%.

[0018] In some examples, the culture includes an isolate and / or a pure culture.

[0019] In some examples, the culture is selected from at least one of a culture broth, a culture broth extract, a whole fungus, a whole fungus extract, a fermentation broth, a fermentation broth extract.

[0020] In another aspect, the present application provides a microbial inoculant, which includes the aforementioned Penicillium oxalicum or culture.

[0021] In some examples, the microbial inoculant further comprises an adjuvant selected from at least one of a solvent, a diluent, a disintegrant, a precipitation inhibitor, a surfactant, a glidant, a binder, a lubricant, a dispersant, a suspending agent, an isotonic agent, a thickening agent, an emulsifying agent, a preservative, a stabilizer, a hydrating agent, an emulsion accelerating agent, a buffer, an absorbent, a coloring agent, a flavoring agent, a sweetening agent, an ion exchange agent, a release agent, a coating agent, a flavoring agent, and an antioxidant.

[0022] In some examples, the dosage form of the microbial inoculant is selected from at least one of a powder, a tablet, a granule, a capsule, a solution, an emulsion, a suspension, an injection, a spray, a powder mist, an aerosol, a suppository, a drop, and a dripping pill.

[0023] In another aspect, the present application provides use of the aforementioned Penicillium oxalicum or culture or microbial inoculant in the preparation of a phosphorus solubilizing product.

[0024] In another aspect, the present application provides a phosphorus solubilizing product comprising the aforementioned Penicillium oxalicum or culture or microbial inoculant.

[0025] In some examples, the phosphorus solubilizing product further comprises at least one of a nutrient agent, a trace element, or a surfactant.

[0026] In some examples, the phosphorus solubilizing product comprises a bio-fertilizer, a phosphorus solubilizing agent, a soil conditioner, a soil amendment, or a soil remediation agent.

[0027] In another aspect, the present application provides use of the aforementioned Penicillium oxalicum or culture or microbial inoculant or phosphorus solubilizing product in soil amendment.

[0028] Specifically, the soil amendment comprises soil conditioning or remediation.

[0029] In some examples, the soil is a soil deficient in soluble phosphorus.

[0030] In another aspect, the present application provides use of the aforementioned Penicillium oxalicum or culture or microbial inoculant or phosphorus solubilizing product in improving soil fertility.

[0031] In some examples, the soil is a soil deficient in soluble phosphorus.

[0032] The Penicillium oxalicum PF1 provided by the present application has salt stress resistance and phosphorus solubilizing function, can improve the utilization rate of insoluble phosphate in saline-alkali soil, significantly increase the content of soluble phosphorus in saline-alkali soil, and has wide application prospect in soil remediation and improvement of soil fertility in saline-alkali soil, and has far-reaching significance in agricultural production activities.

[0033] Deposit information:

[0034] Biological material: PF1;

[0035] Classification name: Penicillium oxalicum Penicillium oxalicum

[0036] Accession No. CGMCC No. 40346

[0037] Deposit date: October 26, 2022

[0038] Depositary: China General Microbiological Culture Collection Center

[0039] Address: No. 1, Xili Beichen, Beijing, China BRIEF DESCRIPTION OF DRAWINGS

[0040] Figure 1 and Figure 2 Growth of Penicillium oxalicum PF1 on a culture plate is shown.

[0041] Figure 3 Phosphorus solubilization rings of Penicillium oxalicum PF1 (from left to right, calcium phosphate, magnesium phosphate, and iron phosphate) are shown.

[0042] Figure 4 Phosphorus standard concentration curve is shown.

[0043] Figure 5 Culture solution pH value and phosphorus content (calcium phosphate) are shown.

[0044] Figure 6 Culture solution pH value and phosphorus content (magnesium phosphate) are shown.

[0045] Figure 7 Culture solution pH value and phosphorus content (iron phosphate) are shown.

[0046] Figure 8 Growth of Penicillium oxalicum PF1 in a salt tolerance test (upper row from left to right, salt content 0%, 2%, 4%, lower row from left to right, salt content 6%, 8%, 10%, 12%) is shown.

[0047] Figure 9 Growth of Penicillium oxalicum PF1 in a salt tolerance test (upper row from left to right, salt content 14%, 15%, 16%, lower row from left to right, salt content 17%, 18%) is shown.

[0048] Figure 10 Growth of Penicillium oxalicum PF1 in a salt tolerance test (upper row, salt content 16%, lower row from left to right, salt content 17%, 18%) is shown. DETAILED DESCRIPTION

[0049] ​Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. For purposes of interpreting this specification, the following definitions will apply and whenever appropriate, terms used in the singular will include the plural and vice versa.

[0050] As used herein, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. For example, reference to "a cell" includes a plurality of such cells and equivalents thereof known to those skilled in the art, and so forth.

[0051] Reagents and materials involved in the present application:

[0052] (a) Test soil sample:

[0053] The test soil sample was collected from the field test station of the Yellow River Delta Comprehensive Test Center of the Chinese Academy of Forestry (118°54'4''E, 37°38'36''N), and the plant was selected as the rhizosphere soil of Tamarix. When collecting, the salt crust and the upper soil layer were removed with a radius of 20 cm from the center of the main stem on the ground, and the underground part of the plant was dug out as a whole. The soil 5-20 cm from the ground was taken and placed in a sterile sample bag. The ice bag was stored at low temperature and transported back to the laboratory for testing.

[0054] (b) Culture medium:

[0055] LB medium: 10 g of proteose peptone, 5 g of yeast extract, 10 g of NaCl, 1000 mL of distilled water, pH 7.0-7.2 (15 g of agar was added to the solid medium on this basis).

[0056] Inorganic phosphorus medium: 10 g of glucose, 0.5 g of yeast extract, 0.5 g of (NH4)2SO4, 0.3 g of NaCl, 0.3 g of KCl, 0.3 g of MgSO4, 0.03 g of MnSO4, 0.03 g of FeSO4, 5 g of phosphorus source, 1000 mL of distilled water, pH 7.0-7.5 (15 g of agar was added to the solid medium on this basis).

[0057] (c) Molybdenum-antimony storage solution: measure 153 mL of concentrated sulfuric acid and slowly add to 400 mL of distilled water, stirring constantly and cooling. Measure another 10 g of finely ground ammonium molybdate and dissolve in 300 mL of deionized water at a temperature of about 60°C, and cool. Then slowly pour the sulfuric acid solution into the ammonium molybdate solution. Add 100 mL of 0.5% antimony potassium tartrate solution, and after cooling, add deionized water to 1 L, shake well, and store in a brown reagent bottle.

[0058] (d) Molybdenum-antimony anti-color developing agent: 100 mL of stock solution plus 1.5 g of ascorbic acid, prepared fresh.

[0059] (e) 5 mg / L phosphorus standard stock solution:

[0060] Accurately weigh potassium dihydrogen phosphate (GB1274, superior pure) dried at 105℃ for 2h, 0.4394g, dissolve with water, add 5mL of concentrated sulfuric acid, add water to 1L, the solution contains 100mg / L of phosphorus, and store in the refrigerator for long-term use.

[0061] Experimental method of the application:

[0062] (f) molybdenum-antimony anti-colorimetric method:

[0063] A standard solution of 0-1.2µg / mL is prepared with potassium dihydrogen phosphate dried to constant weight. In a 25mL sample system, add 1mL of ascorbic acid solution and 2mL of molybdate solution and mix thoroughly. Measure the absorbance at a wavelength of 700nm. Dilute the fermentation filtrate with deionized water to the appropriate multiple for measurement, and use the molybdenum-antimony anti method to quantitatively determine the phosphorus solubilizing ability of the phosphorus solubilizing bacteria.

[0064] (1) Preparation of standard curve: Take 5mL of phosphorus standard solution 0mL, 2mL, 4mL, 6mL, 8mL, 10mL into a 50mL volumetric flask, add the same volume of blank solution as the sample solution used for colorimetric determination, add 2 drops of dinitrophenol indicator, and adjust the solution to just slightly yellow with 100g / L sodium carbonate solution or 50mL / L sulfuric acid solution. Accurately add 5mL of molybdenum-antimony anti-color reagent, shake well, and place at room temperature above 15℃ for 30min. At a wavelength of 700nm, measure the absorbance. Take the absorbance as the vertical coordinate and the phosphorus concentration (mg / L) as the horizontal coordinate to draw the standard curve.

[0065] (2) Determination of soluble phosphorus: Take an appropriate amount of the sample to be tested (fermentation supernatant) into a 50mL volumetric flask, dilute with water to about 3 / 5 of the total volume, add 1-2 drops of dinitrophenol indicator, and adjust the solution to just slightly yellow with 100g / L sodium carbonate solution. Accurately add 5mL of molybdenum-antimony anti-color reagent, shake well, add water to volume, and place at room temperature for 30min. Measure the absorbance of the colored sample at 700nm to calculate the phosphorus content.

[0066] In order to make the objectives, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to examples. If specific conditions are not indicated in the examples, the conventional conditions or the conditions suggested by the manufacturers are used. If the manufacturers of all reagents or instruments are not indicated, the conventional products available in the market are used. In order to better illustrate the present application, numerous specific details are given in the following specific embodiments. The specific embodiments described herein are only used to explain the present application and do not constitute any limitation on the present application. In addition, in the following description, the description of the well-known structures and techniques is omitted to avoid unnecessary confusion of the concept of the present application. Such structures and techniques are described in many publications, for example, "Molecular Cloning: A Laboratory Manual (Fourth Edition)", Cold Spring Harbor Laboratory Press, Ausubel, F. M. et al., "Current Protocols in Molecular Biology", John Wiley & Sons, Inc., and the publications of Greene Publishing Assoc. and Wiley-Interscience. Current Protocols in Molecular Biology

[0067] Example 1 Isolation and screening of Penicillium oxalicum

[0068] 5 g of a soil sample was added to 45 mL of sterile water, and a suspension was prepared by shaking at 30°C and 120 rpm for 30 min. After standing for 15 min, gradient dilution (10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 ) was performed, and 0.1 mL of the suspension was spread on each inorganic phosphorus medium plate. Each gradient was plated in duplicate, and the plates were incubated at 28°C for 5-7 days. Single colonies with obvious transparent circles were picked. Strains with high phosphorus solubilizing ability were selected for purification. After purification for 3 times, single colonies were picked and inoculated on LB slants for 2-3 days for storage.

[0069] As a result of the screening, a strain PF1 was obtained. The sequencing result showed that it was Penicillium oxalicum (P. oxalicum). Penicillium oxalicum The growth of P. oxalicum PF1 on the plate is shown in Figures 1-2 .

[0070] Example 2 Detection of the phosphorus solubilizing ability of P. oxalicum PF1

[0071] The P. oxalicum PF1 strain obtained by isolation and purification was inoculated with single colonies into LB liquid medium, and was incubated overnight (OD 600 was controlled between 0.6 and 0.7) to obtain a bacterial solution.

[0072] 2.1 Phosphorus solubilizing circle (qualitative test)

[0073] ​The inorganic phosphorus solid medium was set with calcium phosphate, iron phosphate, magnesium phosphate as phosphorus source, and the phosphorus concentration was 5 g / L. 20 μL of bacterial liquid was inoculated in the center of the medium, and cultured at 28°C for 10 days. Three parallel samples were set for each phosphorus source. The growth of the strain was observed, and the ratio of the transparent circle diameter D to the colony diameter d was measured to roughly reflect the phosphorus solubilizing ability of the strain. The results are shown in Table 1 and Figure 3 .

[0074] Table 1 D / d value of Penicillium oxalicum PF1 under different phosphorus sources

[0075]

[0076] According to the results of the phosphorus solubilization ring test, the phosphorus solubilizing ability of the strain to different phosphorus sources is calcium phosphate > magnesium phosphate > iron phosphate.

[0077] 2.2 Liquid culture (quantitative test)

[0078] The prepared bacterial liquid was inoculated into the inorganic phosphorus liquid medium at an inoculation amount of 1% (v / v). The phosphorus source was also set as three test groups of calcium phosphate, magnesium phosphate and iron phosphate, and the phosphorus concentration was 5 g / L. Three parallel samples were set for each test group. The CK group was not treated with Penicillium oxalicum. The culture was carried out at 28°C and 180 rpm for 7 days. The supernatant was sampled under sterile conditions every day, and the pH value and soluble phosphorus content (molybdenum antimony resistance colorimetric method) were measured. The determination of phosphorus standard concentration curve is shown in Table 2 and Figure 4 .

[0079] Table 2 Determination of phosphorus standard concentration curve

[0080]

[0081] 15 mL of supernatant diluted to an appropriate concentration was taken in a 50 mL volumetric flask, 2-3 drops of indicator were added, and the solution was adjusted to just yellowish, 5 mL of molybdenum antimony resistance color developing agent was added, shaken and diluted to 50 mL. At a wavelength of 700 nm, the absorbance was measured. The results are shown in Tables 3, 4 and 5.

[0082] Among them, the data in Table 3, 4 and 5 in the parentheses are the data of the CK group under the corresponding conditions.

[0083] Table 3 pH value and phosphorus content of the culture solution (calcium phosphate)

[0084]

[0085] The results (Table 3, Figure 5 ) show that during the 7-day culture period, the pH value of the culture solution is stable between 2 and 3, and the highest phosphorus content is 980.09 mg / L, which is increased by 1507.23% compared with the highest value of the CK group.

[0086] Table 4 pH value and phosphorus content (magnesium phosphate) of culture solution

[0087]

[0088] The results show (Table 4, Figure 6 ), the pH value of the culture solution is stable between 7 and 8, and the highest phosphorus content is 267.20 mg / L, which is 927.30% higher than the highest value of the CK group.

[0089] Table 5 pH value and phosphorus content (iron phosphate) of culture solution

[0090]

[0091] The results show (Table 5, Figure 7 ), the pH value of the culture solution is stable between 2 and 3, and the highest phosphorus content is 17.83 mg / L, which is 60.92% higher than the highest value of the CK group.

[0092] According to the results of the liquid culture test, the phosphorus solubilizing ability of the strains is quite different among different phosphorus sources, and the pH value of the culture solution in the test group is significantly lower than that in the CK group during the phosphorus solubilizing process. The reason may be that the strains release organic acids during the phosphorus solubilizing process, and it is also possible that too low pH has a negative impact on the growth and function of the strains.

[0093] Example 3 Salt tolerance limit test

[0094] The strains were inoculated into LB liquid medium and cultured at 28°C and 180 rpm, and the OD600 value of the bacterial solution was controlled between 0.6 and 0.7, and the shaking was stopped. The salt concentration gradient was set as 0%, 2%, 4%, 6%, 8%, 10%, 12%, 14%, 15%, 16%, 17%, and 18% (w / v) PDA medium, 20 μL of bacterial solution was inoculated in the center of the salt-containing medium, three parallel samples were set for each gradient, the growth of the strains was observed and quantified daily, and the colony diameter was measured after 10 days of culture.

[0095] Table 6 Colony diameter of Penicillium oxalicum PF1 under different salt content

[0096]

[0097] The results show (Table 6, Figure 8 , Figure 9 , Figure 10), inoculated on the medium with 0%-12% salt content, the strain spread and grew vigorously; from 14%, 15% salt content, the growth of the strain began to be inhibited, but still grew normally; under 16%-17% salt content, the growth inhibition was significant, but white colonies were still observed on the medium, and the strain could still survive and colonize; when the salt content was 18%, no colony was observed, and the strain stopped growing.

[0098] From the above related tests, it can be seen that the test strain Penicillium oxalicum PF1 has excellent salt tolerance.

[0099] It should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A Penicillium oxalicum (P. oxalicum) strain, characterized in that, Penicillium oxalicum The preservation date of the Penicillium oxalicum is October 26, 2022, and it is preserved in the China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 40346. ​ 2. A culture, characterized in that, The culture is the Penicillium oxalicum of claim 1 inoculated in a culture medium and cultured.

3. A microbial inoculant, characterized in that, The microbial agent comprises the Penicillium oxalicum of claim 1 or the culture of claim 2.

4. The microbial inoculant of claim 3, wherein, The microbial agent further comprises an adjuvant selected from at least one of a diluent, a disintegrant, a precipitation inhibitor, a surfactant, a glidant, a binder, a lubricant, a dispersant, a suspending agent, an isotonic agent, a thickening agent, an emulsifying agent, a preservative, a stabilizer, an emulsification accelerator, a buffer, an absorbent, a coloring agent, a flavoring agent, a sweetener, an ion exchanger, a release agent, a coating agent, a flavoring agent, and an antioxidant.

5. The microbial inoculant of claim 4, wherein, The dosage form of the microbial agent is selected from at least one of a powder, a tablet, a granule, a capsule, a solution, an emulsion, a suspension, and an aerosol.

6. Use of the Penicillium oxalicum of claim 1, the culture of claim 2, or the microbial agent of any one of claims 3-5 in the preparation of a phosphorus-solubilizing product.

7. A product for dephosphorization, characterized by, The phosphorus-solubilizing product comprises the Penicillium oxalicum of claim 1, the culture of claim 2, or the microbial agent of any one of claims 3-5.

8. The product of claim 7, wherein, The phosphorus-solubilizing product further comprises at least one of a nutrient, a trace element, or a surfactant.

9. The product of dephosphorylation according to claim 7 or 8, characterized in that, The phosphorus-solubilizing product is selected from a bio-fertilizer, a soil conditioner, a soil improver, or a soil remediation agent.

10. Use of the Penicillium oxalicum of claim 1, the culture of claim 2 or the microbial inoculant of any one of claims 3-5 or the phosphorus solubilizing product of any one of claims 7-9 for soil amelioration, characterized in that, The soil improvement comprises soil conditioning or remediation.

Images