Instant composite oligosaccharide solid beverage and preparation method thereof

Abstract

The invention discloses an instant composite oligosaccharide solid beverage and a preparation method thereof. The solid beverage is prepared from the following components in parts by weight: 4 to 8 parts of xylooligosaccharide, 9 to 16 parts of fructo-oligosaccharide, 2 to 4 parts of chitosan oligosaccharide, 5 to 10 parts of galactooligosaccharide, 1 to 2 parts of agaric oligosaccharide, 1 to 2 parts of xanthan gum oligosaccharide, 0 to 0.5 part of citric acid and 0 to 4 parts of erythritol. According to a balanced dietary pagoda and daily dietary fiber recommended intake of a human body, xylooligosaccharide from staple food, fructo-oligosaccharide from fruits and vegetables, chitosan oligosaccharide from animals and galactooligosaccharide from dairy products are adopted; the oligosaccharide solid beverage is prepared by scientifically compounding agaric oligosaccharide from fungi, xanthan gum oligosaccharide from microorganisms and a sour and sweet flavoring agent, and the oligosaccharide solid beverage is good in taste, has the health-care effects of lowering lipid, tonifying the spleen, moistening the intestines and the like, is suitable for overweight old people and has a certain market prospect.

Description

Technical Field

[0001] The present invention relates to the field of nutritional health foods, and particularly relates to an instant compound oligosaccharide solid beverage and a preparation method thereof. Background Art

[0002] Existing dietary fibers are mainly used as food ingredient components in various solid beverages. Some patented products use polysaccharides as the main components to prepare solid beverages, but there are problems such as poor solubility, high viscosity, and indigestibility. As a dietary fiber with a small molecular weight, oligosaccharides are rich in variety, easily soluble in water, easy to digest, and have prebiotic activity. However, there are few solid beverages on the market with oligosaccharides as the main component. Currently, common fructooligosaccharide solid beverages have the disadvantages of simple composition, single function, and easy blood sugar rise.

[0003] The present invention scientifically compounded a solid beverage of oligosaccharides by using xylooligosaccharide from staple foods, fructooligosaccharide from fruits and vegetables, chitosan oligosaccharide from animals, galactooligosaccharide from dairy products, auricularia auricula oligosaccharide from fungi, and xanthan gum oligosaccharide from microorganisms, sour agent citric acid, and sweetener erythritol, which has the effects of reducing lipid and strengthening spleen and moistening intestine, and has a broad market prospect.

[0004] Xanthan gum is an extracellular polysaccharide secreted by Xanthomonas campestris pv. campestris, which has excellent thickening, suspending, emulsifying, and water-soluble properties, and has good thermal and acid-base stability. It is a safe food additive approved by the US Food and Drug Administration (FDA) and has been widely used in various foods. Xanthan gum oligosaccharide is an oligosaccharide compound obtained by enzymatic hydrolysis or other degradation methods of xanthan gum, and is a commercially available safe functional oligosaccharide with prebiotic effects. Compared with other oligosaccharides, xanthan gum oligosaccharide has the advantages of low cost, high safety factor, wide source, wide application range, good biological activity, and high stability, and has broad application prospects in foods. Its application in solid beverages has not been reported at present.

[0005] In solid beverages mainly based on prebiotics, xylooligosaccharide, fructooligosaccharide, chitosan oligosaccharide, and galactooligosaccharide are often added. However, the suitable population for the above oligosaccharides in solid beverages is not clear, and the addition amount of oligosaccharides lacks sufficient experimental data support. The solid beverage in the present invention designs the addition amounts of common staple food source, fruit and vegetable source, animal source, and dairy product source oligosaccharides with reference to the intake of different dietary components in the dietary pagoda, and at the same time adds auricularia auricula oligosaccharide and xanthan gum oligosaccharide from microorganisms. The solid beverage in the present invention takes oligosaccharides as the main component, rationally matches the formula of oligosaccharides with reference to the dietary pagoda, and utilizes the digestibility and functionality of oligosaccharides to develop a solid beverage suitable for overweight elderly people, and at the same time presents a good lipid-lowering effect. Currently, this solid beverage is still in a blank state in the market.

[0006] After retrieving existing patent documents, it was found that CN109170461A discloses a fructooligosaccharide solid beverage and its preparation method; this oligosaccharide solid beverage is prepared from the following raw materials in parts by weight: 50 - 70 parts of arabinose, 20 - 30 parts of polydextrose, 10 - 20 parts of fructooligosaccharide, and 1 - 2 parts of xylooligosaccharide. This product belongs to a new type of sugar substitute in the health industry, has a low calorific value, will not cause obesity when consumed for a long time, and also has the effects of weight loss, blood pressure reduction, blood sugar reduction, and blood lipid reduction, and is more suitable for diabetic patients; the preparation method of this fructooligosaccharide solid beverage is simple and easy to operate, has a low cost, is safe, healthy, and pollution-free, and the prepared fructooligosaccharide solid beverage has both good taste and health care effects.

[0007] CN116250605A discloses a burdock solid beverage rich in 1-kestose, inulin, and fructooligosaccharide, belonging to the technical field of food processing. This invention uses the natural medicinal and edible herb burdock, and through enzymatic hydrolysis treatment of burdock roots, the prepared burdock solid beverage has diverse functional elements, including inulin contained in itself, generated fructooligosaccharides (GF2, GF3, GF4), and potential functional sweetener DFA-I. The contents of inulin, GF2, GF3, GF4, and DFA-I are 13.2%, 1.5%, 1.6%, 1.6%, and 4.6% respectively. The burdock solid beverage prepared by this invention contains multiple prebiotics; at the same time, the burdock solid beverage provided by this invention is packaged in a bag with a small volume, easy to carry and brew, is a convenient, nutritious, and healthy solid beverage product, is a form of deep processing and utilization of burdock, and has a broad market prospect.

[0008] CN104366649A discloses a solid beverage containing probiotics and oligosaccharides and its preparation method. The solid beverage is prepared by mixing skim milk powder, maltodextrin, microcrystalline cellulose, galactooligosaccharide, inulin, sodium carboxymethylcellulose, and probiotic powder in proportion and then making total mixing and packaging into solid beverages. The advantages of this invention are that probiotics have the effects of regulating the balance of intestinal flora, relieving hangover and protecting the liver, and enhancing immunity. Coupled with the presence of functional oligosaccharides inulin and galactooligosaccharide, the colonization and survival rate of probiotics in the intestine are enhanced.

[0009] CN111887370A discloses a solid beverage of marine fish oligopeptide chitosan oligosaccharide for reducing uric acid. The solid beverage of marine fish oligopeptide chitosan oligosaccharide for reducing uric acid of this invention includes the following components: marine fish oligopeptide, chitosan oligosaccharide, tart cherry powder, lemon fruit powder, poria cocos powder, erythritol, polydextrose, sodium citrate. The formula of this invention uses food raw materials, and the main active ingredients in the raw materials are all small molecule substances, which are easily absorbed by the body and have high biological activity. Under scientific proportioning, through different action mechanisms, it can significantly reduce the uric acid level of the body, and has high safety and no any toxic and side effects.

[0010] CN110547468A discloses a preparation method of probiotic microcapsules containing edible mushroom oligosaccharides. After mixing intestinal probiotic liquid, heat-denatured whey protein solution and auricularia auricula oligosaccharide solution, TGase is quickly added, and vortex oscillation is carried out to make it disperse evenly. The mixed solution is added to a conical flask containing preheated soybean oil, and reaction is carried out under the action of a magnetic stirrer. After the system is converted into a gel under the action of the enzyme, centrifugation is carried out, the microcapsule particles are collected and washed twice with Ringer's reagent, and then the colloidal particles are collected again after centrifugation and stored at 4 °C for standby. The invention adds small-molecule edible mushroom oligosaccharides to the wall material, provides a new prebiotic wall material, and has important practical significance for the in-depth processing of edible mushrooms and the exploration of intestinal probiotic embedding technology. Summary of the Invention

[0011] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide an instant compound oligosaccharide solid beverage and a preparation method thereof. The compound solid beverage uses oligosaccharides from various types of food sources, including xylooligosaccharide, fructooligosaccharide, chitosan oligosaccharide, galactooligosaccharide, auricularia auricula oligosaccharide, xanthan oligosaccharide, citric acid, and erythritol, to scientifically compound an oligosaccharide solid beverage. The beverage has a good taste and has health care functions such as reducing lipid and strengthening the spleen and moistening the intestine.

[0012] To achieve this purpose, the present invention adopts the following technical solutions:

[0013] In the first aspect, the present invention provides an instant compound oligosaccharide solid beverage, and the solid beverage includes the following components by weight:

[0014] 9-16 parts of fructooligosaccharide,

[0015] 4-8 parts of xylooligosaccharide,

[0016] 2-4 parts of chitosan oligosaccharide,

[0017] 5-10 parts of galactooligosaccharide,

[0018] 1-2 parts of auricularia auricula oligosaccharide,

[0019] 1-2 parts of xanthan oligosaccharide,

[0020] 0-0.5 part of citric acid,

[0021] 0-4 parts of erythritol.

[0022] Fructooligosaccharide is a functional oligosaccharide formed by the combination of multiple fructose groups through β(2→1) glycosidic bonds with the fructose group in sucrose, and the degree of polymerization is 2-9. It is widely present in various plants, such as bananas, garlic, onions, barley, wheat, and malt, etc. It is a natural active ingredient and also an excellent water-soluble dietary fiber, and has the effect of promoting the proliferation of probiotics.

[0023] Xylooligosaccharides are functional oligosaccharides composed of 2 to 7 xylose molecules linked by β-1,4 glycosidic bonds. Xylooligosaccharides are widely distributed in nature, mainly in fiber raw materials rich in lignin. They have good palatability, a certain degree of sweetness, and their sweetness is similar to that of sucrose.

[0024] Chitosan oligosaccharides are low-molecular polymers obtained by degrading chitosan. Generally, the degree of polymerization is between 2 and 20. Its raw material, chitin, is the most abundant natural resource except for cellulose and is the main component of the outer shells of crustaceans such as crabs and shrimps. Chitosan oligosaccharides have low molecular mass, high deacetylation degree, low viscosity, and complete water solubility, and have various special biological properties such as antibacterial, anti-tumor, and anti-inflammatory effects, and are safe and non-toxic to humans. Since it was approved as a new food raw material in China in 2014, chitosan oligosaccharides, as a food raw material with many properties, have gradually attracted attention.

[0025] Galactooligosaccharides are functional oligosaccharides naturally present in mammalian milk. They are less abundant in animal milk and more abundant in human milk. Galactooligosaccharides are oligosaccharides formed by the action of β-galactosidase on lactose, with 2 to 9 galactosyl groups linked to 1 terminal glucose through glycosidic bonds. Galactooligosaccharides are a type of milk-derived functional oligosaccharide with relatively good dietetic probiotic properties, and have effects such as regulating the intestinal flora, improving intestinal function, immune regulation, and preventing allergies.

[0026] Auricularia auricula oligosaccharides are prepared by hydrolyzing Auricularia auricula polysaccharides, mainly including oligo-glucose linked by β-1,3 glycosidic bonds. Auricularia auricula is one of the important cultivated fungi in China and has important edible value, with the effects of clearing the lungs, moistening the body fluids, and reducing cholesterol. Auricularia auricula oligosaccharides have various biological activities such as hypoglycemic, hypolipidemic, and anti-tumor effects; however, there has been no literature report on its lipid-lowering effect before the present invention. And it is generally considered that adding Auricularia auricula oligosaccharides to solid beverages can be used as dietary fiber to improve intestinal peristalsis.

[0027] Xanthan oligosaccharides are oligosaccharide compounds obtained by biocatalysis or other degradation methods from xanthan gum. Its raw material, xanthan gum, is a microbial extracellular polysaccharide secreted by Xanthomonas campestris pv. campestris, which has excellent thickening, suspending, emulsifying, and water-soluble properties and is a safe food additive approved by the US Food and Drug Administration (FDA). Compared with other oligosaccharides, xanthan oligosaccharides have low cost, high safety factor, wide application range, and good biological activity, and are a type of commercially available safe functional oligosaccharide with prebiotic effects. In addition, xanthan gum and xanthan oligosaccharides have prebiotic potential. The present invention finds that the viscosity of xanthan gum aqueous solution is high and it is not suitable for making solid beverages, while xanthan oligosaccharides have good water solubility and the potential to be applied to solid beverages. The present invention first applies xanthan oligosaccharides to solid beverages.

[0028] Citric acid is a naturally occurring substance present in many fruits such as lemons, oranges, etc. It is an organic weak acid that can provide a fresh and refreshing sour taste, enhancing the flavor of food, and is widely used in the food industry as a flavoring agent, acidulant, preservative, etc.

[0029] Erythritol is a four-carbon polyol widely present in fruits and vegetables, with unique advantages such as low calorie content, pure sweetness, and a refreshing taste. Compared with sucrose, erythritol has a calorie content of only 0.4 kcal / g, its sweetness is about 70% of sucrose, it has a more positive sweetness compared to other sweeteners, no aftertaste of bitterness, and can mask other negative aftertastes such as astringency and the taste of strongly stimulating sweeteners. Therefore, erythritol, as a low-calorie sweetener, is widely used in the food industry.

[0030] In the present invention, the fructooligosaccharide is 9 to 16 parts, for example, it can be 9, 10, 11, 12, 13, 14, 15, 16 parts, etc.

[0031] In the present invention, the xylooligosaccharide is 4 to 8 parts, for example, it can be 4, 5, 6, 7, 8 parts, etc.

[0032] In the present invention, the chitosan oligosaccharide is 2 to 4 parts, for example, it can be 2, 2.5, 3, 3.5, 4 parts, etc.

[0033] In the present invention, the galactooligosaccharide is 5 to 10 parts, for example, it can be 5, 6, 7, 8, 9, 10 parts, etc.

[0034] In the present invention, the auricularia auricula oligosaccharide is 1 to 2 parts, for example, it can be 1.0, 1.2, 1.4, 1.6, 1.8, 2.0 parts, etc.

[0035] In the present invention, the xanthan oligosaccharide is 1 to 2 parts, for example, it can be 1.0, 1.2, 1.4, 1.6, 1.8, 2.0 parts, etc.

[0036] In the present invention, the citric acid is 0 to 0.5 parts, for example, it can be 0, 0.1, 0.2, 0.3, 0.4, 0.5 parts, etc.

[0037] In the present invention, the erythritol is 0 to 4 parts, for example, it can be 0, 0.5, 0.6, 1, 1.5, 2, 2.5, 3.0, 3.5, 4.0 parts, etc.

[0038] The recommended daily intake of dietary fiber for the human body is 25 - 30g. According to the balanced diet pagoda, the recommended intake of staple foods is 250 - 400g, the recommended intake of fruits and vegetables is 500 - 850g, the recommended intake of animal foods is 120 - 200g, and the recommended intake of dairy foods is 300 - 500g. The addition amount of the above fructooligosaccharides refers to the recommended daily intake of dietary fiber and the recommended intake ratio of fruits and vegetables. The addition amount of xylooligosaccharides refers to the recommended daily intake of dietary fiber and the recommended intake ratio of staple foods. Chitosan oligosaccharide refers to the recommended daily intake of dietary fiber and the recommended intake ratio of animal foods. Galactooligosaccharide refers to the recommended daily intake of dietary fiber and the recommended intake ratio of dairy foods. Auricularia auricula oligosaccharide refers to the recommended daily intake of dietary fiber and the recommended intake ratio of fungal foods. Xanthan gum oligosaccharide refers to the recommended daily intake of dietary fiber and the recommended intake for lipid-lowering efficacy.

[0039] The above-mentioned various substances are combined in a specific ratio to achieve a synergistic effect. It not only has the oligosaccharide components from various food sources but also is a compound solid beverage integrating lipid-lowering and nutritional health care.

[0040] In the present invention, the monosaccharide composition of the xanthan gum oligosaccharide is composed of glucose, mannose, and glucuronic acid. Preferably, the purity of the xanthan gum oligosaccharide is 82.15%, and the molecular weight is 1023Da. The molar percentages of glucose, mannose, and glucuronic acid in the monosaccharide composition are 45 - 48.5%: 21.5 - 23%: 30 - 32%. In some implementation examples, the xanthan gum oligosaccharide is prepared by an enzymatic method, including: using atmospheric and room temperature plasma (ARTP) combined with xanthan gum to mutagenize and breed Escherichia coli BL21 expressing MiXen to obtain a strain with an enzyme activity of 18.5U / ml or higher; enzymatically preparing the crude xanthan gum oligosaccharide; separating and purifying, and identifying the xanthan gum oligosaccharide by high-performance liquid chromatography.

[0041] In the present invention, the compound mass ratio of citric acid and erythritol is 1:0 - 8. Preferably, the compound mass ratio of citric acid and erythritol is 1:2 - 6. Most preferably, the compound mass ratio of citric acid and erythritol is 1:5. Example 3 of the present invention is the most preferred scheme. If it is not within this most preferred range, the solid beverage still has the function of improving the intestinal flora, but the lipid-lowering efficacy will be weakened.

[0042] In the present invention, the mass composition of the compound oligosaccharide solid beverage is: water 5.0 - 8.0%, ash 2.0 - 4.0%, protein 0.2 - 0.7%, total sugar 84 - 92%, and reducing sugar 1.2 - 3.5%.

[0043] In the present invention, the dissolution range of the compound oligosaccharide is 20 - 200 mL of water per bag, the dissolution time is 2 - 5 s, and the solution presents a transparent light yellow color.

[0044] Second, the application of the instant compound oligosaccharide solid beverage of the present invention in the preparation of health care products for reducing blood lipid and strengthening the spleen and moistening the intestine; it belongs to the protection scope of the present invention.

[0045] Third, the application of the instant compound oligosaccharide solid beverage of the present invention in the preparation of weight loss and constipation prevention beverages; it also belongs to the protection scope of the present invention.

[0046] The solid beverage of the present invention has the health care functions of reducing blood lipid and strengthening the spleen and moistening the intestine. The solid beverage is suitable for people with hyperlipidemia, obesity and intestinal flora imbalance, especially for overweight elderly people. The drinking amount is generally 20 - 30 g per person per day.

[0047] Compared with the prior art, the present invention has the following beneficial effects:

[0048] The solid beverage of the present invention rationally formulates the usage amounts of fructooligosaccharide, xylooligosaccharide, chitosan oligosaccharide, galactooligosaccharide, auricularia auricula oligosaccharide and xanthan gum oligosaccharide with reference to the balanced diet pagoda and the recommended daily dietary fiber intake for humans. The oligosaccharides from various different food sources cooperate in a specific proportion to play complementary functions and synergistically promote the effects of reducing blood lipid and strengthening the spleen and moistening the intestine. In addition, the oligosaccharide solid beverage of the present invention has the characteristics of small molecular weight, fast dissolution speed, good taste and convenient carrying, and is suitable for the broad health care beverage market. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] By reading the detailed description of the non-limiting embodiments with reference to the following drawings, other features, objects and advantages of the present invention will become more obvious:

[0050] Figure 1 It is a schematic diagram of the appearance morphology of auricularia auricula oligosaccharide;

[0051] Figure 2 It is a schematic diagram of the appearance morphology of xanthan gum oligosaccharide;

[0052] Figure 3 It is a comparison chart of the influence of the solid beverage on the body weight of obese mice;

[0053] Figure 4 It is a comparison chart of the influence of the solid beverage on the perirenal fat weight of obese mice. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0054] In order to further illustrate the technical solution of the present invention, the following describes the specific implementation manners of the present invention in detail with reference to the embodiments. It should be noted that this embodiment is descriptive and not restrictive, and the protection scope of the present invention cannot be limited thereby.

[0055] Preparation Method of Auricularia auricula Oligosaccharide in Example 1

[0056] Weigh 2 g of crude polysaccharide powder of Auricularia auricula commercially available from Zhenjiang Junlin Biotechnology Co., Ltd. and dissolve it in 250 mL of distilled water. Add 1.5 mL of 30% H2O2 solution and treat it in an ice bath for 30 min under the condition of an ultrasonic power of 60 W. After the degradation, use a rotary evaporation device to concentrate the hydrolysis solution to 50 mL, add 100 mL of absolute ethanol, let it stand for 6 h, centrifuge at 5000 rpm for 10 min to remove the undigested polysaccharide, then add 200 mL of absolute ethanol to the supernatant, let it stand for 6 h, and centrifuge at 5000 rpm for 10 min to obtain the oligosaccharide precipitate. Then use a dialysis bag with a molecular weight cut-off of 100 - 500 Da to dialyze to remove H2O2 and freeze-dry it in a vacuum freeze dryer to obtain Auricularia auricula oligosaccharide (see Figure 1 ).

[0057] Preparation Method of Xanthan Gum Oligosaccharide in Example 2

[0058] (1) Inoculate Escherichia coli BL21 expressing the MiXen (endo-xanthanase) gene (provided by Sangon Biotech (Shanghai) Co., Ltd.) onto an LB solid medium and culture it overnight. Then pick a single colony from the LB solid plate and inoculate it into an LB liquid medium, and culture it at 37 °C until the OD of the bacterial suspension 600 is between 0.6 and 0.8 for standby.

[0059] (2) Use atmospheric and room temperature plasma (ARTP) combined with xanthan gum for mutagenesis and screening. Sterilize the ARTP workbench, take 10 μL of the bacterial suspension and place it on a sterilized ARTP slide, adjust the mutagenesis working parameters, the power of the power supply is 120 W, the gas flow rate is 10 L / min, and the mutagenesis time is 80 s. After the mutagenesis treatment, spread the bacterial suspension on a TSB solid medium containing 1% xanthan gum and culture it at 30 °C for about 1 - 2 d, and set up a blank plate as a control at the same time. Pick a single colony on the TSB solid medium and transfer it to the TSB solid medium for streak passage and standby. After 12 - 24 h, transfer it to a shake flask and culture it at 37 °C for 12 h, centrifuge at 5000 rpm for 10 min to obtain the bacterial precipitate, add an appropriate amount of sterile water to suspend it, control the OD of the bacterial suspension 600 to be between 0.8 and 0.9, use an ultrasonic crusher to crush the bacterial suspension, ultrasonicate it for 5 minutes under the condition of 200 W, and centrifuge the bacterial suspension at 5000 rpm for 10 min. The obtained supernatant is the crude enzyme solution.

[0060] (3) Screening for strains with high xylanase activity. Prepare a 2 g / L xanthan gum solution using PBS buffer. Add 50 μL of the above crude enzyme solution to 100 mL of the xanthan gum solution (the control group is the enzyme solution inactivated by boiling water). The enzymatic reaction is carried out at 35 °C for 45 min, and the reaction is terminated by boiling water bath for 10 min. The content of reducing sugar in the hydrolyzate is determined by the DNS method. A strain with an enzyme activity of 18.5 U / ml is screened and named MiXenYB1.

[0061] (4) Preparation of crude xyloglucan oligosaccharides by enzymatic hydrolysis. Add 200 mL of absolute ethanol to 100 mL of the above enzymatic hydrolyzate, let it stand for 6 h, and centrifuge at 5000 rpm for 10 min to remove the unhydrolyzed polysaccharides. Then add 400 mL of absolute ethanol to the supernatant, let it stand for 6 h, and centrifuge at 5000 rpm for 10 min to obtain crude xyloglucan oligosaccharides, which are dried for later use.

[0062] (5) Separating and purifying xyloglucan oligosaccharides using gel chromatography technology.

[0063] ① At room temperature, soak the Bio-p-2 gel packing dry powder of Bio-Rad in 500 mL of distilled water for 24 hours to ensure gel swelling until the gel volume no longer changes;

[0064] ② Pack the swollen gel packing into the chromatography column, and avoid generating air bubbles or faults in the column. The packing should be uniform. Rinse for 12 hours, control the flow rate at 1 mL / min, and compact the packing;

[0065] ③ Dissolve 20 mg of crude xyloglucan oligosaccharides in 0.3 mL of distilled water, filter it with a microporous filter membrane, and then load the sample;

[0066] ④ After loading the sample, take samples every 4 min for 35 tubes;

[0067] ⑤ Use thin-layer chromatography to detect the separation of xyloglucan oligosaccharides. The developing solvent is n-propanol: distilled water = 7:3. Use orcinol staining solution to stain to judge the main components of xyloglucan oligosaccharides, and then collect and freeze-dry for later use;

[0068] (6) Identifying the molecular weight of xyloglucan oligosaccharides using a high-performance liquid chromatography-mass spectrometry (HPLC-MS) instrument. Chromatographic conditions: BRT UPLColigo A chromatographic column (100 mm × 2.1 mm, 3.5 μm), flow rate: 0.3 mL / min, column temperature: 40 °C, injection volume: 10 μL; collect data in positive ion mode. The gradient elution conditions are shown in Table 1.

[0069] Table 1 Mobile phase gradient elution conditions

[0070] Time 0.1% Aqueous Formic Acid Solution (%) Acetonitrile (%) 0 95 5 40 0 100 45 95 5

[0071] (7) Analyze the monosaccharide composition of xanthan oligosaccharides by high performance liquid chromatography (HPLC). The specific method is as follows: Dissolve about 5 mg of the sample in 300 μL of 2 M trifluoroacetic acid, and then hydrolyze it at 110 °C for 1 h. Dry the hydrolyzed sample with nitrogen, add 300 μL of methanol, and then dry it three times to remove trifluoroacetic acid. Dissolve the final hydrolysis product in 25 mL of distilled water for analysis. Determine the monosaccharide composition of the hydrolysis product and the monosaccharide standard on a Dionex ion chromatography system (ICS 5000; Dionex Corp., USA) with a CarboPac PA20 chromatographic column.

[0072] In this example, the xanthan oligosaccharides obtained according to the above steps are as Figure 2 shown. The purity of the xanthan oligosaccharides is 82.15%, the molecular weight is 1023 Da, and the monosaccharide composition is composed of glucose, mannose, and glucuronic acid. The molar percentages between glucose, mannose, and glucuronic acid are 47.49%:21.64%:30.87%.

[0073] Example 3 Preparation of Compound Oligosaccharide Solid Beverage A

[0074] This example provides a compound oligosaccharide solid beverage, which, by weight, includes: 9 parts of fructooligosaccharide; 4 parts of xylooligosaccharide; 2 parts of chitosan oligosaccharide; 5 parts of galactooligosaccharide; 2 parts of auricularia auricula oligosaccharide; 2 parts of xanthan oligosaccharide; 0.1 part of citric acid; 0.5 part of erythritol. After adding the above components in the above proportions, pulverize and mix them thoroughly with an ultrafine pulverizer, pass through a 120-mesh sieve, and package 25 g of the powder into one bag to obtain compound oligosaccharide solid beverage A.

[0075] In this example, the mass percentages of auricularia auricula oligosaccharide and xanthan oligosaccharide are 8.13% and 8.13% respectively. The mass composition of the compound oligosaccharide solid beverage is (Table 2): moisture is 7.3%, ash is 2.6%, protein is 0.4%, total sugar is 86.8%, and reducing sugar is 2.9%.

[0076] Table 2 Mass Composition of Compound Oligosaccharide Solid Beverage

[0077]

[0078]

[0079] Example 4 Preparation of Compound Oligosaccharide Solid Beverage B

[0080] This embodiment provides a compound oligosaccharide solid beverage. The compound solid beverage comprises, by weight: 16 parts of fructooligosaccharide; 8 parts of xylooligosaccharide; 4 parts of chitosan oligosaccharide; 10 parts of galactooligosaccharide; 1.5 parts of auricularia auricula oligosaccharide; 1.5 parts of xanthan gum oligosaccharide; 0.2 part of citric acid; 0.5 part of erythritol. After adding the above components in the said proportions, they are pulverized by an ultrafine pulverizer, fully mixed, sieved through a 120-mesh sieve, and 25 g of the powder is packaged into one bag, thus obtaining the compound oligosaccharide solid beverage B.

[0081] In this embodiment, the mass percentages of auricularia auricula oligosaccharide and xanthan gum oligosaccharide are 2.46% and 2.46% respectively. The mass composition of the compound oligosaccharide solid beverage is as follows (Table 1): moisture is 5.7%, ash is 3.1%, protein is 0.3%, total sugar is 89.1%, and reducing sugar is 1.8%.

[0082] Preparation of Compound Oligosaccharide Solid Beverage C in Example 5

[0083] This embodiment provides a compound oligosaccharide solid beverage. The compound solid beverage comprises, by weight: 13 parts of fructooligosaccharide; 6 parts of xylooligosaccharide; 3 parts of chitosan oligosaccharide; 7 parts of galactooligosaccharide; 1 part of auricularia auricula oligosaccharide; 1 part of xanthan gum oligosaccharide; 0.1 part of citric acid; 0.6 part of erythritol. After adding the above components in the said proportions, they are pulverized by an ultrafine pulverizer, fully mixed, sieved through a 120-mesh sieve, and 25 g of the powder is packaged into one bag, thus obtaining the compound oligosaccharide solid beverage C.

[0084] In this embodiment, the mass percentages of auricularia auricula oligosaccharide and xanthan gum oligosaccharide are 4.59% and 4.59% respectively. The mass composition of the compound oligosaccharide solid beverage is as follows (Table 1): moisture is 6.4%, ash is 3.6%, protein is 0.4%, total sugar is 87.0%, and reducing sugar is 2.6%.

[0085] Preparation of Compound Oligosaccharide Solid Beverage D in Comparative Example 1

[0086] This comparative example provides a compound oligosaccharide solid beverage. The compound solid beverage comprises, by weight: 9 parts of fructooligosaccharide; 4 parts of xylooligosaccharide; 2 parts of chitosan oligosaccharide; 5 parts of galactooligosaccharide; 4 parts of xanthan gum oligosaccharide; 0.1 part of citric acid; 0.5 part of erythritol. After adding the above components in the said proportions, they are pulverized by an ultrafine pulverizer, fully mixed, sieved through a 120-mesh sieve, and 25 g of the powder is packaged into one bag, thus obtaining the compound oligosaccharide solid beverage D.

[0087] Preparation of Compound Oligosaccharide Solid Beverage E in Comparative Example 2

[0088] This comparative example provides a compound oligosaccharide solid beverage. The compound solid beverage comprises, by weight: 9 parts of fructooligosaccharide; 4 parts of xylooligosaccharide; 2 parts of chitosan oligosaccharide; 5 parts of galactooligosaccharide; 4 parts of auricularia auricula oligosaccharide; 0.1 part of citric acid; 0.5 part of erythritol. After adding the above components in the said proportions, they are pulverized by an ultrafine pulverizer, fully mixed, passed through a 120-mesh sieve, and 25 g of the powder is packaged into one bag, thus obtaining the compound oligosaccharide solid beverage E.

[0089] Preparation of the compound oligosaccharide solid beverage F in Comparative Example 3

[0090] This comparative example provides a compound oligosaccharide solid beverage. The compound solid beverage comprises, by weight: 9 parts of fructooligosaccharide; 4 parts of xylooligosaccharide; 2 parts of chitosan oligosaccharide; 5 parts of galactooligosaccharide; 0.1 part of citric acid; 4.5 parts of erythritol. After adding the above components in the said proportions, they are pulverized by an ultrafine pulverizer, fully mixed, passed through a 120-mesh sieve, and 25 g of the powder is packaged into one bag, thus obtaining the compound oligosaccharide solid beverage F.

[0091] Effect of the compound oligosaccharide solid beverage in Example 6 on obese mice

[0092] SPF-grade C57BL / 6 mice were housed in ventilated cages at 25 ± 1 °C, under a cycle of 12 h light / 12 h darkness, with free access to water and food. After a 1-week adaptation period, the mice were randomly divided into a normal group and a model group. Normal group: The mice were fed with regular food. Model group: The mice were fed with HFD. After 4 weeks, when the body weight of the mice in the model group increased by 10%, they became obese model group mice, and their body weights were recorded. The obese model group mice were divided into 4 groups, namely a control group, a solid beverage A group, a solid beverage B group, and a solid beverage C group, with 6 mice in each group. The above 4 groups were respectively gavaged with 200 mg / kg.bw of drinking water, 200 mg / kg.bw of solid beverage A, 200 mg / kg.bw of solid beverage B, and 200 mg / kg.bw of solid beverage C for 8 consecutive weeks. The normal group drank normal drinking water every day, and the body weights of all mice were recorded every 2 weeks. After the experiment, the mice were humanely euthanized by inhaling 5% isoflurane, and the perirenal fat was collected and weighed.

[0093] As Figure 3 shown, compared with the obese model group mice, the body weights of the mice in the treatment groups of Comparative Example 1, Comparative Example 2, and Comparative Example 3 showed a decreasing trend, and the body weights of the mice in Comparative Example 1 and 2 decreased significantly. Compared with the obese model group mice, after the obese mice were gavaged with solid beverage A, solid beverage B, and solid beverage C, their body weights decreased significantly. Among them, the amount of body weight decrease of the mice treated with solid beverage A was the most significant, and it was also significantly greater than the amount of body weight decrease of the mice treated with Comparative Example 1, Comparative Example 2, and Comparative Example 3. The above results indicate that solid beverage A shows the best weight loss effect. As Figure 4As shown, compared with the mice in the obesity model group, the perirenal fat weight of the mice in the treatment groups of Comparative Example 1, Comparative Example 2 and Comparative Example 3 decreased slightly. The perirenal fat weight of the obese mice treated with Solid Beverage A and Solid Beverage B showed a significant decrease, and was significantly greater than the decrease in the perirenal fat weight of the mice in the treatment groups of Comparative Example 1, 2 and 3. Among them, Solid Beverage A showed the best lipid-lowering effect. From the above results, it can be seen that compared with Comparative Example 3, the obese mice showed a slight lipid-lowering effect after adding xanthan oligosaccharide and auricularia auricula oligosaccharide in Comparative Example 1 and 2 respectively. After adding xanthan oligosaccharide and auricularia auricula oligosaccharide simultaneously in Solid Beverage A, Solid Beverage B and Solid Beverage C, they showed better weight loss and lipid-lowering effects compared with Comparative Example 1, 2 and 3. Among them, Solid Beverage A had the best weight loss and lipid-lowering effect.

[0094] The applicant declares that the instant compound oligosaccharide solid beverage and its preparation method of the present invention are illustrated by the above embodiments, but the present invention is not limited to the above embodiments. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, making equivalent substitutions or changes, should be covered within the protection scope and disclosure scope of the present invention.

Claims

1. An instant compound oligosaccharide solid beverage, characterized in that, The raw materials are measured by weight, including: 9-16 parts of oligofructose, 4-8 parts of xylo-oligosaccharide, 2-4 parts of chitosan oligosaccharide, Galacto-oligosaccharide 5-10 parts, 1-2 portions of fungus oligosaccharide, 1-2 parts of xanthan gum oligosaccharide, 0-0.5 parts of citric acid, 0-4 parts of erythritol.

2. The instant compound oligosaccharide solid beverage according to claim 1, wherein The monosaccharide composition of the xanthan gum oligosaccharide is composed of glucose, mannose and glucuronic acid.

3. The instant compound oligosaccharide solid beverage according to claim 2, wherein The molar percentages of glucose, mannose and glucuronic acid in the monosaccharide composition of the xanthan gum oligosaccharide are 45-48.5%: 21.5-23%: 30-32%.

4. The instant compound oligosaccharide solid beverage according to claim 1, wherein The compounding mass ratio of the citric acid and erythritol is 1:0-8.

5. The instant compound oligosaccharide solid beverage according to claim 4, wherein The compounding mass ratio of the citric acid and erythritol is 1:2-6.

6. The instant compound oligosaccharide solid beverage according to claim 1, wherein The mass composition of the composite oligosaccharide solid beverage is: 5.0-8.0% of water, 2.0-4.0% of ash, 0.2-0.7% of protein, 84-92% of total sugar and 1.2-3.5% of reducing sugar.

7. The instant compound oligosaccharide solid beverage according to claim 1, wherein The solubility range of the complex oligosaccharide is 20-200 mL of water / bag, the dissolution time is 2-5 seconds, and the solution is transparent light yellow.

8. Use of the instant composite oligosaccharide solid beverage according to any one of claims 1 to 7 in the preparation of lipid-lowering and spleen-strengthening and intestinal-moistening health products.

9. Use of the instant composite oligosaccharide solid beverage according to any one of claims 1 to 7 in the preparation of a beverage for weight loss and constipation prevention.

10. The application according to claim 8 or 9, characterized in that, The drinking amount of the solid beverage is 20 to 30 g / person / day.

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