Oil control composition as well as application and cosmetic thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses an oil control composition and application thereof and cosmetics, the oil control composition is obtained by water extraction of a plant combination of bitter almond seeds, reeds, radix ophiopogonis roots and rhizoma kaempferiae roots, and the weight ratio of the bitter almond seeds to the reeds to the radix ophiopogonis roots to the rhizoma kaempferiae roots in the plant combination is (3-7): (4-8): (3-7): (1-6); when the four plant extracts are used together, the four plant extracts can play a synergistic role, act on the skin through multiple pathways, reduce synthesis and secretion of lipid in sebaceous gland cells and decompose skin grease, and have a higher 5alpha-reductase inhibition rate and an excellent oil control effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and specifically discloses an oil-control composition, its use, and a cosmetic product. Background Art

[0002] In modern society, people's attention to skin health and appearance is increasing day by day. People with oily skin face many troubles. Excessive sebum secretion not only makes the skin look shiny, affecting beauty, but also easily causes skin problems such as clogged pores, acne, and comedones, bringing negative impacts to personal image and mental health. At the same time, when the skin is exposed to external stimuli, such as ultraviolet rays, environmental pollution, and improper skin care, it is prone to discomfort symptoms such as sensitivity, flushing, and itching. Currently, there are various oil-control products and technologies on the market. Traditional oil-control products mostly use adsorbents, such as talcum powder, kaolin, etc., to reduce the oil on the skin surface through physical adsorption. However, this method only temporarily solves the surface problem and cannot regulate sebum secretion from the root, resulting in poor oil-control effect. And long-term use may cause burden on the skin, affecting the normal respiration and metabolism of the skin. Chemical synthetic oil-control ingredients, although they can regulate sebum secretion to a certain extent, may be irritating and easily cause adverse reactions such as skin allergies, and are not suitable for sensitive skin.

[0003] Based on this, the technical problem to be solved by the present invention is how to prepare an oil-control product with high oil-control efficacy. Summary of the Invention

[0004] The purpose of the present invention is to provide an oil-control composition containing bitter almond seed extract, reed extract, ophiopogon root extract, and kaempferia root extract. Through the synergistic effect among the four, it can effectively reduce sebum secretion and achieve the purpose of oil control.

[0005] At the same time, the present invention also provides the use of an oil-control composition and a cosmetic product.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] An oil-control composition, wherein the oil-control composition is obtained by water extraction of a plant combination of bitter almond seeds, reeds, ophiopogon roots, and kaempferia roots, and the weight ratio of bitter almond seeds, reeds, ophiopogon roots, and kaempferia roots in the plant combination is 3-7:4-8:3-7:1-6.

[0008] In the present invention, the effects of each component are as follows:

[0009] Bitter almond seed extract: Bitter almond seed extract is rich in various vitamins, fatty acids and minerals. It can help regulate the activity of sebaceous glands by supplementing the nutrients of the skin lipid barrier. Its emollient property can reduce the phenomenon of excessive oil secretion caused by water shortage while moisturizing the skin, thus indirectly achieving the effect of oil control;

[0010] Reed extract: Flavonoids and other compounds in reed extract have the effect similar to phytoestrogens. They can combine with hormone receptors in human skin cells to regulate the balance of hormones in the body, reduce the sensitivity of sebaceous glands to androgens, and thus reduce the amount of oil secretion.

[0011] Ophiopogon japonicus root extract: Components such as homoisoflavonoids in Ophiopogon japonicus root extract can act on sebaceous gland cells. By affecting the signal transduction pathway in cells, it reduces the expression of genes related to lipid synthesis and secretion in sebaceous gland cells, and then reduces the ability of sebaceous glands to synthesize and secrete oil, controls the production of skin oil from the source, makes the skin oil secretion at a normal level, and achieves the effect of oil control.

[0012] Kaempferia galanga root extract: Kaempferia galanga root extract has a certain inhibitory effect on some common skin microorganisms such as Propionibacterium acnes and Malassezia. When these microorganisms overgrow on the skin surface, they will decompose the oil on the skin surface, produce irritating substances, cause skin inflammation, and then lead to abnormal oil secretion. Kaempferia galanga root extract maintains the balance of skin microecology by inhibiting the growth and reproduction of these microorganisms, reduces skin inflammation and abnormal oil secretion caused by microbial infection, and makes the skin more refreshing and comfortable.

[0013] In the research of the present invention, it is found that when the above four plant extracts are used together, they can play a synergistic effect. They may act on the skin through multiple pathways, reduce the synthesis and secretion of lipids in sebaceous gland cells and decompose skin oil, and exert an excellent oil control effect.

[0014] Preferably, the extraction method of the extract is as follows:

[0015] Step 1: After crushing the plant combination of bitter almond seeds, reeds, Ophiopogon japonicus roots and Kaempferia galanga roots through a 40-mesh sieve, add water and stir at 65 ± 5 °C for 2 - 4 h, centrifuge to remove the precipitate, and concentrate the centrifugate under reduced pressure at 60 ± 5 °C and -0.08 ± 0.01 MPa to 25 - 35 wt% of the mass of the centrifugate to obtain the first concentrated solution. Among them, the weight ratio of the plant combination to water is 1 - 3:8 - 15;

[0016] Step 2: Add an equal volume of 95 vol% ethanol to the first concentrated solution, perform alcohol precipitation for 12 - 24 h, centrifuge to remove the precipitate, and concentrate the centrifugate under reduced pressure at 60 ± 5°C and -0.08 ± 0.01 MPa until there is no alcohol left to obtain a second concentrated solution; add butanediol and water to the second concentrated solution, stir evenly, and perform ultrafiltration at 0.25 ± 0.1 MPa to obtain an ultrafiltrate, where the weight ratio of the second concentrated solution, butanediol, and water is 1 - 3:3 - 5:5 - 7;

[0017] Step 3: Add a preservative to the ultrafiltrate and sterilize it at 85 ± 3°C for 0.5 - 1 h to obtain an oil-control composition.

[0018] In addition, the present invention also provides the use of the above-mentioned oil-control composition in preparing cosmetics.

[0019] Preferably, the cosmetics have an oil-control effect.

[0020] Finally, the present invention also discloses a cosmetic containing the above-mentioned oil-control composition.

[0021] Preferably, the cosmetic contains 0.01 - 30 wt% of the oil-control composition.

[0022] More preferably, the dosage form of the cosmetic is lotion, cream, essence, lotion, shampoo, facial foam, facial cleanser or hair conditioner.

[0023] Compared with the prior art, the beneficial effects of the present invention are:

[0024] The oil-control composition of the present invention is obtained by combining extracts of bitter almond seeds, reed, root of dwarf lilyturf, and root of lesser galangal. When these four plant extracts are used together, they have a synergistic effect of enhancing the oil-control effect. Description of the Drawings

[0025] Figure 1 It is an observation diagram of a chicken embryo before using the sample in Example 1;

[0026] Figure 2 It is an observation diagram of a chicken embryo after using the sample in Example 1. Detailed Embodiments

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] Product information:

[0029] Bitter almond seeds: with a moisture content of 4.8 wt%, purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd.;

[0030] Reed: with a moisture content of 6.1 wt%, purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0031] Ophiopogon japonicus roots: with a moisture content of 13.5 wt%, purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd.;

[0032] Kaempferia galanga roots: with a moisture content of 9.3 wt%, purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd.

[0033] The preparation methods of the oil-control compositions in each example and comparative example are as follows:

[0034] Step 1: After crushing the plant combination of bitter almond seeds, reed, Ophiopogon japonicus roots and Kaempferia galanga roots through a 40-mesh sieve, add it to water and stir at 65 °C for 3 h. Centrifuge to remove the precipitate, and concentrate the centrifugate by vacuum distillation at 60 °C and -0.08 MPa to 30 wt% of the mass of the centrifugate to obtain the first concentrated solution. Among them, the weight ratio of the plant combination to water is 2:10;

[0035] Step 2: Add an equal volume of 95 vol% ethanol to the first concentrated solution, perform alcohol precipitation for 18 h, centrifuge to remove the precipitate, and concentrate the centrifugate by vacuum distillation at 60 °C and -0.08 MPa until alcohol-free to obtain the second concentrated solution; add butanediol and water to the second concentrated solution, stir evenly, and perform ultrafiltration at 0.25 ± 0.1 MPa to obtain the ultrafiltrate. Among them, the weight ratio of the second concentrated solution, butanediol and water is 2:3:5;

[0036] Step 3: Add 0.5% of 1,2-hexanediol and 0.5% of p-hydroxyacetophenone, which is equivalent to 0.5% of the weight of the ultrafiltrate, to the ultrafiltrate, and sterilize at 85 °C for 1 h to obtain the oil-control composition.

[0037] The formulations of the plant combinations in each example and comparative example are shown in Table 1;

[0038] Table 1 Formulation table of plant combinations (parts by weight)

[0039] Bitter almond seeds Reed Ophiopogon japonicus root Kaempferia galanga root Example 1 5 6 5 4 Example 2 3 8 3 6 Example 3 7 4 7 1 Comparative Example 1 0 6 5 9 Comparative Example 2 9 6 5 0 Comparative Example 3 0 10.5 9.5 0

[0040] Performance testing

[0041] Chorioallantoic membrane test of chicken embryo

[0042] 1. Test purpose and principle

[0043] The chicken embryo chorioallantoic membrane (CAM) test is an early - adopted in - vitro evaluation method for eye irritation. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chicken embryo. This test utilizes the characteristics of the intact, clear, and transparent vascular system of the chorioallantoic membrane in the mid - stage of incubated chicken embryos. A certain amount of the test substance is directly contacted with the chorioallantoic membrane of the chicken embryo. After a period of action, the changes in the toxicity effect indicators of the chorioallantoic membrane (such as bleeding, coagulation, and vascular lysis) are observed. These indicators reflect the changes in the morphological structure, color, and permeability of blood vessels and vascular networks, as well as the phenomena of protein denaturation in the chorioallantoic membrane and its degree of damage. Then, a score is combined to evaluate the eye irritation of the test substance.

[0044] The purpose of this test is to test the ability of the test substance to cause toxic changes in the chicken embryo chorioallantoic membrane and to evaluate the elements and processes of the potential eye irritation of the substance to be evaluated.

[0045] 2. Test materials and methods

[0046] 2.1 Instrument and equipment

[0047] Fully automatic incubator;

[0048] Stereomicroscope;

[0049] SPF chicken embryos.

[0050] 2.2 Reagents

[0051] Sodium chloride;

[0052] Sodium dodecyl sulfate (SDS).

[0053] 2.3 Incubation conditions

[0054] Room temperature: 20°C - 25°C, relative humidity: 45% - 70%. Incubation temperature: 37.5°C ± 0.5°C, relative humidity: 55% - 70%, turntable rotation frequency: 3 times / h - 6 times / h. Chicken embryos at 9 - day - old do not need to be rotated during incubation.

[0055] 2.4 Test methods

[0056] (1) Test operation steps

[0057] In this test, 6 embryos are selected for each group. The situation of the chorioallantoic membrane is recorded with a photographing device. The composition of Example 1 is diluted with pure water into a 2% aqueous solution, added to the chorioallantoic membrane of the chicken embryo. The time of adding the sample is recorded, and the air chamber is covered with a moistened plastic wrap. The chicken embryo is transferred to an incubator with constant temperature and humidity for cultivation. The degree of change in each toxicity effect is observed and photographed.

[0058] (2) Result observation

[0059] Observe and record the manifestations of bleeding, coagulation, and vascular lysis, and score according to their severity.

[0060] (3) Data analysis

[0061] Table 2 Scoring criteria

[0062]

[0063]

[0064] The experiment was conducted using the endpoint evaluation method. The endpoint evaluation (ES) was calculated, and the result was retained to two decimal places; ES ≤ 4, no irritation; 4 < ES ≤ 12, mild irritation; 12 < ES < 16, moderate irritation; ES ≥ 16, strong irritation / corrosion; the score of each chick embryo = the sum of the degrees of bleeding, blood coagulation, and vascular lysis observed in each chick embryo; ES = the average value of the mathematical sum obtained from 6 chick embryos.

[0065] After detection, the ES of the composition in Example 1 was 2.67, indicating that the sample was mild and non-irritating. Refer to Figure 1 、 Figure 2 , for the comparison of the chick embryo states before and after dropping the aqueous solution prepared from the composition of Example 1 onto the chick embryo. Among them, Figure 1 is the observation diagram of the chick embryo before using the sample, Figure 2 is the observation diagram of the chick embryo after using the sample.

[0066] 5α-reductase inhibition rate

[0067] 1. Test purpose and principle

[0068] The sebaceous glands of oily skin have relatively strong secretion functions. The face feels greasy, is not easy to clean, and has an oily sheen that affects beauty. At the same time, some corresponding skin diseases are prone to occur, such as acne vulgaris and seborrheic dermatitis, etc. In view of the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of the test sample on 5α-reductase is usually detected to reflect the role of sebum regulation, so as to characterize the oil control effect of the test sample. In this test, referring to the laboratory method, the 5α-reductase inhibition rate test results of the test sample and the negative control were compared. If the inhibition rate of the test sample was higher than that of the negative control, it could be considered that the test sample had a certain oil control effect.

[0069] 2. Test materials and methods

[0070] 2.1 Instrument and equipment

[0071] BSA224S analytical balance;

[0072] Agilent 1220 high performance liquid chromatograph;

[0073] 2.2 Reagents

[0074] Testosterone, with a concentration of 98%;

[0075] NADPH, with a concentration of 90%;

[0076] Methanol, with a purity of HPLC grade;

[0077] Buffer: Tris-HCl.

[0078] 2.3 Test methods

[0079] (1) Treatment of reference substances and test samples

[0080] Sample group: Dilute the compositions of Examples 1-3 and Comparative Examples 1-3 with buffer to a sample concentration of 5%;

[0081] Negative reference substance: Tris-HCl buffer.

[0082] (2) Test operation steps

[0083] Set up a sample group, a negative control group and a blank group. Two parallels need to be set up for each group. Add different reagent solutions to the three groups respectively, shake well, perform HPLC analysis on each group, and determine the testosterone content in each tube.

[0084] (3) Calculation formula

[0085]

[0086] 3 Test results

[0087] Table 3 5α-reductase inhibition rate

[0088] Sample Test result / % Example 1 28.89 Example 2 26.25 Example 3 26.78 Comparative Example 1 15.41 Comparative Example 2 13.38 Comparative Example 3 10.24 Negative control 2.03

[0089] Note: The 5α-reductase inhibition rate is retained to two decimal places.

[0090] Data analysis of Table 3 shows that:

[0091] The data of Examples 1-3 show that the combination of bitter almond seed extract, reed extract, ophiopogon root extract and kaempferia root extract used in the present invention can efficiently inhibit 5α-reductase within the weight ratio range of the present invention. The inhibition rate of Example 1 is 28.89%;

[0092] According to the data analysis of Example 1 and Comparative Examples 1-3, when the bitter almond seed extract and / or the kaempferia root extract are missing in the composition, the 5α-reductase inhibition rate is significantly reduced. Especially in Comparative Example 3, which lacks both the bitter almond seed extract and the kaempferia root extract, the 5α-reductase inhibition rate is only 10.24%, much lower than that of Example 1. The above data show that the four components of bitter almond seed extract, reed extract, ophiopogon root extract, and kaempferia root extract in the oil-control composition of the present invention are compounded to produce a synergistic effect of increasing the 5α-reductase inhibition rate, thus achieving a better oil-control effect.

[0093] Application Example

[0094] The present invention discloses a cosmetic (oil-control essence), which contains the oil-control composition prepared in Example 1, and its formula is shown in Table 4;

[0095] Table 4 Formula Table of Oil-Control Essence

[0096]

[0097]

[0098] A preparation method of a cosmetic (oil-control essence):

[0099] (1) Prepare the prefabricated phase:

[0100] ① Phase A3 is pre-stirred and dispersed evenly.

[0101] ② Phase B is pre-stirred and dissolved completely.

[0102] ③ Phase C is heated to 55-60 °C and pre-stirred and dissolved completely, then cooled for standby.

[0103] (2) Take a clean beaker, put the room-temperature Phase A1 into the cup, stir and dissolve it completely, and slowly sprinkle Phase A2 into the liquid surface of the beaker in multiple small amounts until Phase A2 is completely wetted without white powder particles.

[0104] (3) Add the dispersed Phase A3, stir and heat to 85-88 °C, keep warm for 20 minutes, and homogenize appropriately for 1-2 minutes.

[0105] (4) After observing that the material is completely dissolved, cool it to 55-60 °C, add Phases B and C, stir and mix evenly, continue to cool to 45-48 °C, and add Phase D and stir and mix evenly to obtain the oil-control essence.

[0106] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above-described exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present invention.

Claims

1. An oil-control composition, characterized in that, The described oil-control composition is obtained by water extraction from a plant combination of bitter almond seeds, reed, Ophiopogon japonicus roots, and Kaempferia galanga roots. The weight ratio of bitter almond seeds, reed, Ophiopogon japonicus roots, and Kaempferia galanga roots in the plant combination is 3 - 7:4 - 8:3 - 7:1 - 6.

2. The oil-control composition according to claim 1, wherein The extraction method of the described oil-control composition is as follows: Step 1: After crushing the plant combination of bitter almond seeds, reed, Ophiopogon japonicus roots, and Kaempferia galanga roots and passing it through a 40-mesh sieve, add it to water and stir at 65 ± 5°C for 2 - 4 h. Centrifuge to remove the precipitate, and concentrate the centrifugate under reduced pressure at 60 ± 5°C and -0.08 ± 0.01 MPa to 25 - 35 wt% of the mass of the centrifugate to obtain a first concentrated solution. Among them, the weight ratio of the plant combination to water is 1 - 3:8 - 15; Step 2: Add an equal volume of 95% ethanol to the first concentrated solution, perform alcohol precipitation for 12 - 24 h, centrifuge to remove the precipitate, and concentrate the centrifugate under reduced pressure at 60 ± 5°C and -0.08 ± 0.01 MPa until alcohol-free to obtain a second concentrated solution; add butanediol and water to the second concentrated solution, stir evenly, and perform ultrafiltration at 0.25 ± 0.1 MPa to obtain an ultrafiltrate. Among them, the weight ratio of the second concentrated solution, butanediol, and water is 1 - 3:3 - 5:5 - 7; Step 3: Add a preservative to the ultrafiltrate and sterilize it at 85 ± 3°C for 0.5 - 1 h to obtain the oil-control composition.

3. Use of the oil-control composition according to any one of claims 1 - 2 for preparing cosmetics.

4. The use according to claim 3, characterized in that, The described cosmetics have an oil-control effect.

5. A cosmetic, characterized in that, Contain the oil-control composition according to any one of claims 1 - 2.

6. The cosmetic according to claim 5, characterized in that, The cosmetics contain 0.01 - 30 wt% of the oil-control composition.

7. The cosmetic according to claim 5, characterized in that, The dosage form of the described cosmetics is lotion, cream, essence, lotion, shampoo, facial cleansing mousse, facial cleanser, or hair conditioner.

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