Wet compressing agent for external application of postpartum eczema and preparation method of wet compressing agent

Abstract

The invention relates to the technical field of biological medicine, and discloses a wet compressing agent for external application of postpartum eczema and a preparation method of the wet compressing agent for external application of postpartum eczema. 10-20 parts of radix paeoniae alba; 15 to 25 parts of cortex dictamni; 15-25 parts of mulberry leaves; the synergistic component is prepared from the following components in parts by mass: 5 to 15 parts of radix scutellariae, 8 to 15 parts of folium ilicis purpurea; 5 to 10 parts of centella asiatica extract; 3-8 parts of a matricaria recutita extract; the traditional Chinese medicines such as cassia twig, radix paeoniae alba, cortex dictamni, folium mori and folium ilicis purpurea form a basic formula, and the traditional Chinese medicines have the effects of warming and dredging meridians and collaterals, nourishing blood and astringing yin, clearing heat and drying dampness, dispelling wind and detoxifying and the like, and can be used for treating the pathologic characteristics related to excessive postpartum eczema, deficiency of qi and blood, wind-damp-heat evil invasion and the like; the traditional Chinese medicine composition has the effects of strengthening the body resistance to eliminate pathogenic factors, conditioning the internal environment of the body and relieving eczema symptoms, and the targeted treatment effect of the medicine on postpartum eczema is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to a wet compress for external application of postpartum eczema and a preparation method thereof. Background Art

[0002] Wet compresses are a type of topical preparation that exerts therapeutic effects by applying to the skin surface. They are mainly used to relieve symptoms such as skin inflammation, itching, redness and swelling. They are commonly used in the treatment of skin diseases such as eczema and dermatitis. Traditional wet compresses are mostly Chinese herbal decoctions or Western hormone ointments. They achieve local anti-inflammatory and antipruritic effects by penetrating the skin with drugs, but they have problems such as single ingredients, unstable efficacy, easy recurrence, and safety risks (such as hormone dependence).

[0003] However, the traditional decoctions, due to high-temperature decoction and simple filtration, lead to a large loss of fat-soluble active ingredients (such as baicalin and matricolide), and the residual macromolecular impurities (colloids, starch) affect transdermal absorption, resulting in a cure rate of only 41.2% and a recurrence rate of up to 28.6%. Especially for pregnant women after childbirth, postpartum eczema is a skin disease that parturients are susceptible to due to factors such as changes in hormone levels and decreased immunity after childbirth. Existing drugs for the treatment of postpartum eczema may have the problem of not being targeted enough, and do not fully consider the special physical conditions of postpartum women and the specific pathological mechanisms of eczema.

[0004] Therefore, it is necessary to provide a wet compress for external application of postpartum eczema and a preparation method thereof to solve the above problems. Summary of the invention

[0005] The purpose of the present invention is to provide a technical solution to solve the problems in the prior art mentioned in the above background technology.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] A wet compress for external application of postpartum eczema, comprising a basic formula, wherein the basic formula is calculated in parts by mass as follows:

[0008] Cinnamon twig: 10-20 parts;

[0009] White peony root: 10-20 parts;

[0010] Dictamni: 15-25 portions;

[0011] Mulberry leaves: 15-25 parts;

[0012] Evergreen: 8-15 servings.

[0013] Preferably, the wet compress further comprises a synergistic component, wherein the synergistic component is calculated in the following parts by mass:

[0014] Scutellaria baicalensis: 5-15 parts;

[0015] Centella asiatica extract: 5 - 10 parts;

[0016] Matricaria chamomilla extract: 3 - 8 parts;

[0017] Dipotassium glycyrrhizinate: 1 - 5 parts.

[0018] Preferably, the wet compress also includes functional excipients, and the functional excipients are calculated by mass parts as follows:

[0019] Nano zinc oxide: 1 - 3 parts;

[0020] Menthol-β-cyclodextrin inclusion complex: 0.5 - 2 parts.

[0021] Preferably, the content of baicalin extract in Scutellaria baicalensis is 8% - 15%, the content of asiaticoside in Centella asiatica extract is 5% - 10%, and the content of matricarin in Matricaria chamomilla extract is 2% - 5%.

[0022] A preparation method of a wet compress for external application to postpartum eczema, using a wet compress for external application to postpartum eczema, comprising the following steps:

[0023] S1. Raw material pretreatment:

[0024] Selection of raw materials: Use a vibrating screen to remove impurities from Ramulus Cinnamomi, Radix Paeoniae Alba, and Cortex Dictamni. Remove the outer rough bark of Ramulus Cinnamomi, retain the inner bark and xylem; scrape off the surface cork of Radix Paeoniae Alba, only retain the white fleshy part; remove the internal lignified fibers of Cortex Dictamni, retain the light yellow root bark.

[0025] Crushing of raw materials: Use a crusher to coarsely crush Ramulus Cinnamomi, Radix Paeoniae Alba, Cortex Dictamni, and Folium Mori in S1 to 2 - 3 mm particles, and separately perform ultrafine crushing on Ilex purpurea Hassk. to a particle size ≤ 180 microns.

[0026] Sterilization of raw materials: Use a cobalt-60 γ-ray irradiator to irradiate Cortex Dictamni in S2 for 2 hours.

[0027] S2. Alcohol extraction process:

[0028] Dynamic infiltration treatment of raw materials: Mix the crushed Ramulus Cinnamomi, Radix Paeoniae Alba, and Ilex purpurea Hassk. in S2, put them into a multi-functional extraction tank and stir, add 6 times the amount of 70% ethanol, control the temperature at 45°C, and stir at 30 revolutions per minute for 45 minutes.

[0029] First-stage reflux extraction: Heat the multi-functional extraction tank to 78°C, which is the boiling point of ethanol, maintain a slightly boiling state for 1.5 hours, monitor the density of the extract in real time, stop when it reaches 1.12 g / cm³, and recover the volatilized ethanol through a condenser.

[0030] Secondary countercurrent extraction: Add 4 times the amount of fresh 70% ethanol to the medicinal residues in the multi-functional extraction tank after S5 treatment, extract for 1 hour for the second time, combine the two extraction liquids, and concentrate to a relative density of 1.25 to obtain the ethanol extraction concentrate.

[0031] S3. Water extraction process:

[0032] Pressurized decoction: Using a concentration unit, mix dictamnus bark, mulberry leaves, and skullcap root, add 10 times the amount of purified water, and put them into the closed extraction tank of the concentration unit. Pressurize the closed extraction tank of the concentration unit to 0.15 MPa, decoct 3 times, 1 hour each time, and combine the decoction liquids.

[0033] Enzymatic treatment: Add cellulase to the closed extraction tank, adjust the pH to 4.8, enzymatically hydrolyze at a temperature of 50 °C for 30 minutes in the closed extraction tank, and then raise the temperature to 90 °C and maintain for 10 minutes.

[0034] Membrane filtration and purification: Use a 0.2-micron ceramic membrane for filtration, with an operating pressure of 0.3 MPa, and control the filtrate flow rate at 80 L / (m²·h) to remove macromolecular impurities such as colloids and starches to obtain the water extraction concentrate.

[0035] S4. Refining and forming treatment:

[0036] Use a high-pressure homogenizer to mix the ethanol extraction concentrate and the water extraction concentrate at a volume ratio of 3:7, stir at 1200 rpm for 20 minutes until uniform, add nano-zinc oxide, adjust the pH of the mixed solution with 10% citric acid solution and 5% sodium hydroxide solution, add menthol-β-cyclodextrin inclusion complex, and stir in a 5 °C constant temperature water bath until the solution is clear.

[0037] S5. Wet compress agent forming:

[0038] Preparation of drug-loaded substrate: Prepare a mixed colloidal solution of 2% sodium alginate and 1% sodium carboxymethylcellulose, coat it on the surface of a glass plate with a thickness of 0.15 mm, and soak it in a 2% calcium chloride solution for 30 minutes to form a hydrogel film.

[0039] Vacuum impregnation with drug: Uniformly coat the medicinal liquid on the surface of the hydrogel film and perform vacuum impregnation

[0040] Freeze-drying: Use a freeze-dryer to quickly freeze the medicinal liquid on the hydrogel film at -40 °C for 4 hours to form an ice crystal skeleton, and then perform sublimation drying to prepare the formed wet compress agent.

[0041] The technical effects and advantages of the present invention: A wet compress agent for external application in postpartum eczema and its preparation method proposed by the present invention have the following advantages compared with the prior art:

[0042] The present invention uses traditional Chinese medicines such as Cinnamomi Ramulus, Paeoniae Radix Alba, Dictamni Cortex, Mori Folium, and Ilicis Purpureae Folium to form a basic formula. These traditional Chinese medicines have the effects of warming and dredging meridians, nourishing blood and astringing yin, clearing heat and drying dampness, expelling wind and detoxifying, etc. They can target the pathological characteristics of postpartum eczema, which is mostly related to qi and blood deficiency, invasion of wind, dampness, heat, and pathogenic factors, play the role of strengthening healthy qi and eliminating pathogenic factors, regulating the internal environment of the body, and relieving eczema symptoms, and enhance the targeted therapeutic effect of the medicine on postpartum eczema.

[0043] Moreover, to further improve the curative effect, the technical solution adds synergistic components such as Scutellaria baicalensis, Centella asiatica extract, Matricaria recutita extract, and dipotassium glycyrrhizinate. Scutellaria baicalensis has the effects of clearing heat and drying dampness, purging fire and detoxifying, and its active ingredient baicalin has anti-inflammatory, antibacterial and other effects, which can enhance the therapeutic effect on eczema. Asiaticoside in Centella asiatica extract can promote the synthesis of skin collagen and contribute to the repair of eczema skin lesions. Matricarin in Matricaria recutita extract has anti-inflammatory and anti-allergic effects, which can reduce the inflammatory reaction and itching symptoms of eczema. Dipotassium glycyrrhizinate has anti-inflammatory, anti-allergic, moisturizing and other effects, can soothe the skin, and cooperate with other drugs to play a better therapeutic role, thereby enhancing the overall curative effect of the medicine. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 It is a process flow chart for the preparation of a wet compress agent for external application to postpartum eczema according to the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0045] Now the subject matter described herein will be discussed with reference to example embodiments. It should be understood that discussing these embodiments is only to enable those skilled in the art to better understand and thus implement the subject matter described herein, and the functions and arrangements of the elements discussed can be changed without departing from the scope of protection of the content of this specification. Each example can omit, substitute or add various processes or components as needed. In addition, the features described in some examples can also be combined in other examples.

[0046] The invention provides as Figure 1 shown:

[0047] Example 1: Wet compress agent for acute eczema

[0048] Formulation composition (by mass):

[0049] 15 parts of Cinnamomi Ramulus, 15 parts of Paeoniae Radix Alba, 20 parts of Dictamni Cortex, 20 parts of Mori Folium, 10 parts of Ilicis Purpureae Folium;

[0050] 12 parts of Scutellaria baicalensis (baicalin content ≥ 12%), 8 parts of Centella asiatica extract (asiaticoside ≥ 8%);

[0051] 5 parts of Matricaria recutita extract (matricarin ≥ 3%), 3 parts of dipotassium glycyrrhizinate;

[0052] 2 parts of nano-zinc oxide (particle size D50 = 50 nm) and 1.5 parts of menthol-β-cyclodextrin inclusion complex.

[0053] The preparation method of the wet compress agent includes the following steps:

[0054] S1. Raw material pretreatment:

[0055] Selection and purification of raw materials: Use a vibrating sieve to remove impurities from cinnamon twig, white peony root, and dictamnus bark. Remove the outer rough bark of cinnamon twig, retain the inner bark and xylem; scrape off the surface cork of white peony root, only retain the white fleshy part; remove the internal lignified fibers of dictamnus bark, retain the light yellow root bark.

[0056] Crushing of raw materials: Use a crusher to coarsely crush cinnamon twig, white peony root, dictamnus bark, and mulberry leaf in S1 to 2-3 mm particles, and separately ultra-finely crush ivy to a particle size ≤ 180 microns.

[0057] Sterilization of raw materials: Use a cobalt-60 γ-ray irradiator to irradiate dictamnus bark in S2 for 2 hours.

[0058] S2. Ethanol extraction process:

[0059] Dynamic soaking treatment of raw materials: Mix the crushed cinnamon twig, white peony root, and ivy in S2, put them into a multi-functional extraction tank and stir, add 6 times the amount of 70% ethanol, control the temperature at 45°C, and stir at 30 revolutions per minute for 45 minutes.

[0060] First-stage reflux extraction: Heat the multi-functional extraction tank to 78°C, which is the boiling point of ethanol, maintain a slightly boiling state for 1.5 hours, monitor the density of the extract in real time, stop when it reaches 1.12 g / cm³, and recover the volatilized ethanol through a condenser.

[0061] Second-stage countercurrent extraction: Add 4 times the amount of fresh 70% ethanol to the medicinal residues in the multi-functional extraction tank after S5 treatment, extract for 1 hour for the second time, combine the two extracts, and concentrate to a relative density of 1.25 to obtain an ethanol extraction concentrate.

[0062] S3. Water extraction process:

[0063] Pressurized decoction: Use a concentration unit. After mixing dictamnus bark, mulberry leaf, and scutellaria baicalensis, add 10 times the amount of pure water and put it into the closed extraction tank of the concentration unit. Pressurize the closed extraction tank of the concentration unit to 0.15 MPa, decoct 3 times, 1 hour each time, and combine the decoction liquids.

[0064] Enzymatic hydrolysis treatment: Add cellulase to the closed extraction tank, adjust the pH to 4.8, enzymatically hydrolyze at a temperature of 50°C for 30 minutes in the closed extraction tank, and then raise the temperature to 90°C and maintain for 10 minutes.

[0065] Membrane filtration purification, using a 0.2-micron ceramic membrane for filtration, with an operating pressure of 0.3 MPa, controlling the filtrate flow rate at 80 L / (m²·h), removing macromolecular impurities such as colloid and starch, and obtaining a water extract concentrate;

[0066] S4, Refining and shaping treatment:

[0067] Use a high-pressure homogenizer to mix the ethanol extract concentrate and the water extract concentrate at a volume ratio of 3:7, and stir at 1200 rpm for 20 minutes until uniform. Add nano-zinc oxide, adjust the pH of the mixed solution with 10% citric acid solution and 5% sodium hydroxide solution, add menthol-β-cyclodextrin inclusion complex, and stir in a 5°C constant temperature water bath until the solution is clear;

[0068] S5, Preparation of wet compress:

[0069] Preparation of the drug-loaded substrate, prepare a mixed colloidal solution of 2% sodium alginate and 1% sodium carboxymethylcellulose, coat it on the surface of a glass plate with a thickness of 0.15 mm, and soak it in 2% calcium chloride solution for 30 minutes to form a hydrogel film;

[0070] Vacuum impregnation with drug, evenly coat the drug solution on the surface of the hydrogel film and carry out vacuum impregnation

[0071] Freeze-drying, use a freeze-dryer to rapidly freeze the drug solution on the hydrogel film at -40°C for 4 hours to form an ice crystal skeleton, and then carry out sublimation drying to prepare a formed wet compress.

[0072] Example 2: Wet compress for chronic eczema (long-acting repair type)

[0073] Formulation composition (by mass parts):

[0074] 18 parts of Cinnamomum cassia, 15 parts of Paeonia lactiflora, 22 parts of Dictamnus dasycarpus, 25 parts of Morus alba, 12 parts of Ilex purpurea;

[0075] 15 parts of Scutellaria baicalensis (baicalin ≥ 15%), 10 parts of Centella asiatica extract (asiaticoside ≥ 10%);

[0076] 8 parts of Matricaria chamomilla extract (matricarin ≥ 5%), 5 parts of dipotassium glycyrrhizinate;

[0077] 3 parts of nano-zinc oxide (particle size D50 = 30 nm), 2 parts of menthol-β-cyclodextrin inclusion complex

[0078] Example 3: Safe wet compress for lactation period (low irritation type)

[0079] 12 parts of Cinnamomum cassia, 18 parts of Paeonia lactiflora, 25 parts of Dictamnus dasycarpus, 15 parts of Morus alba, 8 parts of Ilex purpurea;

[0080] 5 parts of Scutellaria baicalensis (baicalin ≥ 8%), 5 parts of Centella asiatica extract (asiaticoside ≥ 5%);

[0081] 3 parts of Matricaria recutita extract (Matricarin ≤ 2%) and 1 part of dipotassium glycyrrhizinate;

[0082] 1 part of nano-zinc oxide (particle size D50 = 100 nm) and 0.5 part of menthol-β-cyclodextrin clathrate;

[0083] It should be noted that the preparation methods of the wet compress agents in Example 2 and Example 3 both adopt the preparation method of Example 1;

[0084] Experimental example:

[0085] Taking 120 eczema patients after 4 weeks of treatment as an example for the comparison of clinical efficacy, as shown in the following table:

[0086]

[0087]

[0088] Taking the verification of the low irritation of the lactation-safe wet compress agent in Example 3 as an example, as shown in the following table:

[0089] Test items 3 groups of examples 1 group of examples Group of desonide cream <![CDATA[TEWL value (g / h / m 2 )]]> 8.2±1.3 9.5±1.6 15.7±2.1 Erythema index (a value) 12.3±2.1 14.5±3.0 28.7±4.5 Incidence rate of stinging pain 0％ 3.8％ 22.5％

[0090] Note:

[0091] TEWL (transepidermal water loss): The skin barrier function of the group in Example 3 is the best. Compared with the desonide group: P < 0.01, indicating that hormonal drugs significantly damage the skin barrier.

[0092] Summary: This technical solution shows significant advantages in staged treatment and safety breakthroughs in clinical applications. The preparation in Example 1 designed for acute eczema, through the synergistic effect of high-concentration baicalin (≥12%) and menthol transdermal promoter, achieves a cure rate of 68.3% and an EASI score reduction rate of 84.7% within 4 weeks. Its curative effect is close to that of the hormonal drug desonide cream (cure rate of 75.6%), but the recurrence rate is only 9.7% (32.4% in the desonide group), significantly reducing the risk of hormone dependence.

[0093] The preparation in Example 2 is optimized for chronic lichenified skin lesions. It uses chitosan-modified nano-zinc oxide to target and deliver asiaticoside (≥10%), promoting collagen regeneration and epidermal barrier repair, reducing the 6-month recurrence rate to as low as 6.3%, and at the same time controlling the transepidermal water loss value (TEWL) at 8.2 g / h / m 2 , compared with the recurrence rate of 52.6% and the TEWL value of 15.7 g / h / m of the traditional decoction 2 has obvious advantages.

[0094] For the special group during lactation, in Example 3 preparation, through precise regulation of ingredients (matricaria lactone ≤ 2%, baicalin ≤ 8%), while ensuring a cure rate of 63.5%, double safety for mother and infant is achieved: no migration of active ingredients is detected in breast milk (detection limit 0.01 ppb), the drug residue in the infant's serum is zero, and there are no differences in breastfeeding behavior and infant development indicators compared with the control group. In contrast, due to process defects, the traditional decoction has serious loss of fat-soluble ingredients, and the cure rate is only 41.2%. Although hormonal drugs have significant short-term efficacy, they cause damage to the skin barrier (TEWL value increases by 2 times) and a high recurrence rate of 32.4%.

[0095] The embodiments of the present invention have been described above, but the present invention is not limited to the above specific embodiments. The above specific embodiments are merely illustrative rather than restrictive. Under the inspiration of the present invention, those of ordinary skill in the art can also make many forms, all of which fall within the protection scope of the present invention.

Claims

1. A wet compress agent for external application in postpartum eczema, characterized in that, It includes a basic formula, and the basic formula is as follows by mass fraction: Ramulus Cinnamomi: 10 - 20 parts; Radix Paeoniae Alba: 10 - 20 parts; Cortex Dictamni: 15 - 25 parts; Folium Mori: 15 - 25 parts; Folium Isatidis: 8 - 15 parts.

2. The wet compress agent for external application for postpartum eczema according to claim 1, wherein, It also includes a synergistic component, and the synergistic component is as follows by mass fraction: Radix Scutellariae: 5 - 15 parts; Centella asiatica extract: 5 - 10 parts; Matricaria recutita extract: 3 - 8 parts; Dipotassium glycyrrhizinate: 1 - 5 parts.

3. The wet compress agent for external application for postpartum eczema according to claim 1, wherein It also includes a functional excipient, and the functional excipient is as follows by mass fraction: Nano zinc oxide: 1 - 3 parts; Menthol-β-cyclodextrin inclusion compound: 0.5 - 2 parts.

4. A wet compress agent for external application in postpartum eczema according to claim 2, characterized in that, The content of baicalin extract in the Radix Scutellariae is 8% - 15%, the content of asiaticoside in the Centella asiatica extract is 5% - 10%, and the content of matricarin in the Matricaria recutita extract is 2% - 5%.

5. A preparation method of a wet compress agent for external application in postpartum eczema, using a wet compress agent for external application in postpartum eczema as described in any one of claims 1-4, characterized in that, It includes the following steps: S1. Raw material pretreatment: Selection of raw materials: Use a vibrating sieve to remove impurities from Ramulus Cinnamomi, Radix Paeoniae Alba, and Cortex Dictamni. Remove the outer rough bark of Ramulus Cinnamomi, retain the inner bark and xylem; scrape off the surface cork of Radix Paeoniae Alba, only retain the white fleshy part; remove the internal lignified fibers of Cortex Dictamni, retain the pale yellow root bark. Crushing of raw materials: Use a crusher to coarsely crush Ramulus Cinnamomi, Radix Paeoniae Alba, Cortex Dictamni, and Folium Mori in S1 to 2 - 3 mm particles, and separately ultra - finely crush Folium Isatidis to a particle size ≤ 180 microns. Sterilization of raw materials: Use a cobalt - 60 γ - ray irradiator to irradiate Cortex Dictamni in S2 for 2 hours. S2. Ethanol extraction process: Dynamic soaking treatment of raw materials: Mix the crushed Ramulus Cinnamomi, Radix Paeoniae Alba, and Folium Isatidis in S2, put them into a multi - functional extraction tank and stir, add 6 times the amount of 70% ethanol, control the temperature at 45°C, and stir at 30 revolutions per minute for 45 minutes. First - stage reflux extraction: Heat the multi - functional extraction tank to 78°C, which is the boiling point of ethanol, maintain a slightly boiling state for 1.5 hours, monitor the density of the extract in real - time, stop when it reaches 1.12 g / cm³, and recover the volatilized ethanol through a condenser. Second - stage counter - current extraction: Add 4 times the amount of fresh 70% ethanol to the medicinal residues in the multi - functional extraction tank after S5 treatment, extract for 1 hour for the second time, combine the two extraction liquids, and concentrate to a relative density of 1.25 to obtain an ethanol - extracted concentrated solution. S3. Water extraction process: Pressurized decoction: Use a concentration unit. After mixing Cortex Dictamni, Folium Mori, and Radix Scutellariae, add 10 times the amount of pure water and put them into the closed extraction tank of the concentration unit. Pressurize the closed extraction tank of the concentration unit to 0.15 MPa, decoct 3 times, 1 hour each time, and combine the decoction liquids. Enzymatic hydrolysis treatment: Add cellulase to the closed extraction tank, adjust the pH to 4.8, enzymatically hydrolyze at 50°C for 30 minutes in the closed extraction tank, and then raise the temperature to 90°C and maintain for 10 minutes. Membrane filtration and purification: Use a 0.2 - micron ceramic membrane for filtration, with an operating pressure of 0.3 MPa, control the filtrate flow rate at 80 L / (m²·h), remove macromolecular impurities such as colloids and starches, and obtain a water - extracted concentrated solution. S4. Refining and forming treatment: Use a high-pressure homogenizer to mix the ethanol extract concentrate and the water extract concentrate at a volume ratio of 3:7, and stir at 1200 revolutions per minute for 20 minutes until homogeneous. Add nano-zinc oxide, adjust the pH of the mixed solution with 10% citric acid solution and 5% sodium hydroxide solution, add menthol-β-cyclodextrin inclusion complex, and stir in a 5°C constant temperature water bath until the solution is clear; S5. Preparation of wet compress: Preparation of drug-loaded substrate: Prepare a mixed colloidal solution of 2% sodium alginate and 1% sodium carboxymethylcellulose, coat it on the surface of a glass plate with a thickness of 0.15 mm, and soak it in 2% calcium chloride solution for 30 minutes to form a hydrogel film; Vacuum impregnation with drug: Uniformly coat the liquid medicine on the surface of the hydrogel film and carry out vacuum impregnation Freeze-drying: Use a freeze-dryer to quickly freeze the liquid medicine on the hydrogel film at -40°C for 4 hours to form an ice crystal skeleton, and then carry out sublimation drying to prepare a formed wet compress.

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