Mongolian medicine for treating rhinitis and preparation method and application thereof

Abstract

The invention relates to a Mongolian medicine for treating rhinitis and a preparation method and application thereof. The preparation method comprises the following steps: performing high-energy ball milling on a buffalo horn, bezoar, sea buckthorn root and ligusticum wallichii mixture to obtain a mixture a; placing a mixture of stigma croci, dianthus superbus, flos caryophylli, red clover roots and origanum vulgare leaves above a mixed solution composed of ethanol, dichloromethane and N, N-dimethylformamide for fumigation, collecting fumigation liquid obtained by fumigation through condensation, and performing vacuum freeze drying on the obtained condensate to obtain a mixture b; and mixing the mixture a and the mixture b to obtain the Mongolian medicine for treating rhinitis. The Mongolian medicine is assisted by medicinal materials for clearing heat, detoxifying, diminishing swelling, astringing and conditioning voxels, dredging channels and collaterals and eliminating qi and blood stagnation, and the components in the Mongolian medicine are mutually promoted and assisted, so that the prepared Mongolian medicine is high in treatment and cure rate of allergic rhinitis, not easy to relapse and low in side effect, and is proved to be excellent in clinical curative effect.

Description

Technical Field

[0001] The invention belongs to the technical field of Mongolian medicines, and in particular relates to a Mongolian medicine for treating rhinitis and a preparation method and application thereof. Background Art

[0002] Allergic rhinitis is a common disease. It is not easy to cure and often causes symptoms such as nasal congestion and runny nose. Current drugs have great side effects, long and slow treatment courses, and great side effects. In terms of clinical symptoms, allergic rhinitis manifests as paroxysmal sneezing, clear watery nasal discharge, intermittent or persistent nasal congestion, and strong itching in the nose. When making a diagnosis, it is necessary to determine whether it belongs to intermittent allergic rhinitis or persistent allergic rhinitis. According to traditional Chinese medicine, this disease belongs to the category of "nasal congestion". Its onset is due to weak lung qi, weak defense surface, loose pores, and the invasion of wind and cold evil. The wind and cold evil invades the nasal cavity, the evil and the righteous fight each other, the lung qi is blocked, and the body fluids stagnate, resulting in nasal congestion, sneezing, runny nose and other symptoms.

[0003] At present, the commonly used treatment methods can be selected according to individual specificity, including drug therapy, immunotherapy and surgical treatment. Drug therapy can be administered intranasally or systemically, and commonly used oral or nasal antihistamines, glucocorticoids, anti-leukotrienes, chromatin drugs, intranasal decongestants, intranasal anticholinergics, and Chinese medicine prescriptions; immunotherapy is suitable for situations where conventional drug treatment is ineffective, using subcutaneous injection and sublingual administration to induce clinical and immune tolerance, and local and systemic adverse reactions may occur; surgical treatment is suitable for special cases such as no improvement after drug or immunotherapy, obvious anatomical variations in the nasal cavity accompanied by functional disorders, and combined with chronic rhinosinusitis-nasal polyps.

[0004] Traditional Chinese medicine has a reliable effect on rhinitis treatment, with few toxic and side effects, and has broad prospects. However, judging from the therapeutic effects of traditional Chinese medicines currently published, there are problems such as long treatment course and slow efficacy, and most of them are external medicines, focusing on treating the symptoms and prone to relapse. In this regard, Mongolian medicine has a variety of therapeutic drugs and therapies, and enjoys a high reputation among the people. However, due to the lack of summary, many prescriptions are only circulated among the people in the form of proven prescriptions, which limits the popularization and development of such treatment techniques. Therefore, it is imperative to actively collect, organize and develop new Mongolian medicines that are effective for allergic rhinitis. Summary of the invention

[0005] In view of the technical problems existing in the prior art, the purpose of the present invention is to provide a Mongolian medicine for treating rhinitis and a preparation method and application thereof.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] The present invention provides a method for preparing a Mongolian medicine for treating rhinitis, comprising the following steps:

[0008] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong by weight parts and perform high-energy ball milling to obtain mixture a;

[0009] (2) Cut saffron, dianthus superbus, clove, red clover root and oregano leaf by weight parts, wash, dry and mix evenly. Spread the mixture flat above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and perform vacuum freeze-drying on the condensate to obtain mixture b;

[0010] (3) Mix mixture a and mixture b evenly to obtain a Mongolian medicine for treating rhinitis.

[0011] Furthermore, in the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong, saffron, dianthus superbus, clove, red clover root, oregano leaf is 1-3:5-6:1-1.5:1-3:5-6:1-3:1-3:1-3:1-3.

[0012] Furthermore, in step (1), the rotation speed of the high-energy ball milling is 350-400 r / min, and the high-energy ball milling time is 2-2.5 h.

[0013] Furthermore, in step (2), the volume ratio of ethanol, dichloromethane and N,N-dimethylformamide is 1:(5-10):(5-10).

[0014] Furthermore, in step (2), the drying temperature is 80-120 °C, and the drying time is 3-6 h.

[0015] Furthermore, the fumigation temperature is 180 °C.

[0016] Furthermore, the fumigation is usually to spread the medicinal materials flat on a sieve plate with mesh holes. The aperture of the sieve plate ensures that the medicinal materials will not leak, and the fumigation solvent is located at a certain distance below the sieve plate.

[0017] Furthermore, the Mongolian medicine dosage form can be: tablets, sugar-coated tablets, film-coated tablets, enteric-coated tablets, capsules, hard capsules, soft capsules, oral liquids, buccal tablets, granules, infusion, honey pills, powders, pills, solution, injections, suppositories, syrups, powders, powder injections or sustained-release capsules.

[0018] The present invention provides a Mongolian medicine for treating rhinitis, and the Mongolian medicine is prepared by the above preparation method.

[0019] The present invention also provides an application of a Mongolian medicine for treating rhinitis. Specifically, the Mongolian medicine is prepared by the above method and is used for treating allergic rhinitis.

[0020] The present invention mixes buffalo horn, bezoar, seabuckthorn root and chuanxiong rhizome, and adopts the method of high-energy ball milling to fully release the active ingredients in buffalo horn, bezoar, seabuckthorn root and chuanxiong rhizome, improve the chemical reaction activity of the medicinal materials, and at the same time enable the active ingredients to react and bond under the conditions of high-energy ball milling, so as to obtain the active ingredients of Mongolian medicine that can effectively treat allergic rhinitis. The applicant has found that not all medicinal materials can achieve the improvement of the efficacy of Mongolian medicine in treating allergic rhinitis by the method of high-energy ball milling. Selecting specific buffalo horn, bezoar, seabuckthorn root and chuanxiong rhizome for high-energy ball milling can make the medicinal materials better exert their medicinal properties compared with other medicinal materials and other mixing methods, and thus can better treat allergic rhinitis.

[0021] The present invention uses a mixed solvent to fumigate the mixture of saffron, dianthus superbus, clove, red clover root and oregano leaf, and collects the fumigation liquid after fumigating the mixture by means of condensation. Compared with a single solvent, the selection of the mixed solvent in the present invention can extract the active ingredients for treating allergic rhinitis in the medicinal materials with higher efficiency. By means of the mixed solvent and fumigation, the active ingredients in the mixed medicinal materials are extracted, the purity of the active ingredients for treating allergic rhinitis in Mongolian medicine is improved, and thus the efficiency of Mongolian medicine in treating allergic rhinitis is improved. At the same time, the safety of Mongolian medicine in treating allergic rhinitis is improved, and the occurrence of drug side effects is reduced.

[0022] The present invention adopts the method of vacuum freeze-drying to dry the fumigation product. Compared with other drying methods, vacuum freeze-drying can better maintain the activity of the extracted active ingredients and better exert the medicinal effect of the active ingredients in treating allergic rhinitis.

[0023] The beneficial effects of the present invention:

[0024] The formula of the present invention is simple, the production process is simple, the toxic and side effects are small, and the cost is low. Assisted by medicinal materials for clearing heat, detoxifying, detumescence, astringing and conditioning the body constitution, dredging the meridians and eliminating qi and blood stasis, the components in Mongolian medicine are mutually reinforcing and enhancing. The prepared Mongolian medicine has a high cure rate for treating allergic rhinitis, is not easy to relapse, has low side effects, and has been proven to have very good clinical efficacy.

[0025] The present invention uses medicinal materials with different medicinal properties and conducts scientific proportioning. The applicant unexpectedly finds that adopting a specific treatment method to extract the active ingredients in specific medicinal materials can achieve an ideal treatment effect, can directly reach the lesion, has a short cure time, and is not easy to relapse after cure. Detailed implementation mode

[0026] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0027] Unless otherwise specified, the raw materials and equipment used in the embodiments of the present invention are raw materials and equipment from conventional sources in the technical field. In the ranges disclosed herein, the endpoints and any values are not limited to the exact ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoint values of each range, between the endpoint values of each range and individual point values, and between individual point values can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed herein.

[0028] Pharmacological toxicity test

[0029] Data of acute toxicity experiment

[0030] 1. Experimental method

[0031] Apply the depilatory evenly on both sides of the spine on the back of the guinea pigs, so that the depilated area is about 20 square centimeters. Wash the depilatory and put the guinea pigs back into the cage for observation for 24 hours. Each group of guinea pigs is respectively applied with 0.2, 0.4 and 0.8 ml of the Mongolian medicine preparation external use drops of the present invention, containing 121 mg, 242 mg and 484 mg of crude drugs respectively. Another group is applied with 0.8 ml of the solvent twice a day for one week. After the experiment, the guinea pigs are sacrificed, and the heart, liver, kidney and depilated skin are subjected to pathological examination.

[0032] 2. Results

[0033] In the trunk depilated areas of the above three groups of guinea pigs treated with drugs, there were no local skin edema, congestion, erythema, bleeding points or ulcers. There were no obvious differences in the hair color, food intake, limb activities, etc. of the guinea pigs in the drug treatment groups compared with the control group. In the pathological histological examination, there were also no obvious differences in the heart, liver, kidney and depilated skin of the drug administration groups compared with the control group. The results suggest that the Mongolian medicine external preparation of the present invention has no local irritation effect and no systemic toxicity manifestation. It shows that the traditional Chinese medicine external preparation of the present invention is safe to use.

[0034] Data of long-term toxicity experiment

[0035] 1. Experimental method

[0036] Randomly divide the guinea pigs into 4 groups, with 20 in each group. Apply the depilatory evenly on both sides of the spine on the back of the guinea pigs, so that the depilated area is about 20 square centimeters. Wash the depilatory, and after observing for 24 hours, each group of guinea pigs is respectively applied with 0.2, 0.4 and 0.8 ml of the Mongolian medicine preparation oral capsule of the present invention, containing 121, 242 and 484 mg of crude drugs respectively. Another group is applied with 0.8 ml of the solvent twice a day for 30 days. Observe the general conditions of the guinea pigs. After the experiment, the animals are sacrificed for hematology, blood biochemistry and pathological examinations.

[0037] 2. Results

[0038] In the depilated areas of the trunks of the guinea pigs in the above three groups of medicated animals, no local skin edema, congestion, erythema, bleeding points or ulcers were observed. There were no obvious differences in hair color, food intake, limb movement, etc. between the medicated group and the control group. In the blood biochemical examination, both the medicated group and the control group were within the normal range. In the pathological histological examination, no obvious lesions were found in the heart, liver, kidneys and local skin of each experimental group. It is indicated that long-term administration of the Mongolian medicine preparation of the present invention has no obvious toxic effects on the local skin and important organs of the whole body. The test results show that the Mongolian medicine preparation of the present invention is safe in the experiment.

[0039] Example 1:

[0040] A preparation method of a Mongolian medicine for treating rhinitis comprises the following steps:

[0041] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong according to parts by weight and carry out high-energy ball milling to obtain mixture a;

[0042] (2) Cut crocus sativus, dianthus superbus, cloves, red clover root and oregano leaves according to parts by weight, wash, dry and mix evenly. Spread the mixture flat above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b;

[0043] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0044] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong, crocus sativus, dianthus superbus, cloves, red clover root and oregano leaves is 1:5:1:1:5:1:1:1:1.

[0045] Further, in step (1), the rotation speed of the high-energy ball milling is 350 r / min, and the high-energy ball milling time is 2 h.

[0046] Further, in step (2), the volume ratio of ethanol, dichloromethane and N,N-dimethylformamide is 1:5:5.

[0047] Case 1: Ms. Wang, 32 years old, has had severe allergic rhinitis for 6 years. She has taken a variety of Chinese and Western medicine preparations but all have been ineffective. The main symptoms are: nasal congestion, frequent runny nose and sneezing, and frequent colds.

[0048] Treatment: The granules made from the Mongolian medicine of the present invention are taken twice a day. The effect can be seen after 2 days, and there is a feeling of relaxation in the nose. After continuous use for 6 days, the nose can breathe normally, and the symptoms of running nose and sneezing are significantly reduced. Basically, one can fall asleep normally at night. Continue to use until two courses of treatment (7 days for one course). The symptoms of sneezing and running nose have completely disappeared, and one can fall asleep normally like ordinary people at night. The allergic rhinitis has been cured and has not recurred since then. Moreover, during this period, one catches a cold very rarely and no side effects have been found.

[0049] Example 2:

[0050] A preparation method of a Mongolian medicine for treating rhinitis, comprising the following steps:

[0051] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong according to parts by weight and carry out high-energy ball milling to obtain mixture a;

[0052] (2) Cut crocus sativus, dianthus superbus, cloves, red clover root and oregano leaves according to parts by weight, wash and dry them, then mix them evenly. Spread the mixture above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b;

[0053] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0054] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong, crocus sativus, dianthus superbus, cloves, red clover root and oregano leaves is 1:5:1:1:5:1:1:1:1.

[0055] Further, in step (1), the rotation speed of the high-energy ball milling is 400 r / min, and the high-energy ball milling time is 2 h.

[0056] Further, in step (2), the volume ratio of ethanol, dichloromethane and N,N-dimethylformamide is 1:5:5.

[0057] Case 2: Mr. Zhang, 28 years old, has suffered from severe allergic rhinitis for 1 year. He has taken various Chinese and Western medicine preparations but all have no effect. The main symptoms are: running clear nose constantly in the early morning, itchy nose and sneezing, often blocked in both nostrils, accompanied by headache symptoms.

[0058] Treatment: The granules made from the Mongolian medicine of the present invention are taken twice a day. After two courses of treatment, it is cured without any adverse symptoms and has not recurred since then.

[0059] Comparative Example 1:

[0060] A preparation method of a Mongolian medicine for treating rhinitis, comprising the following steps:

[0061] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong rhizome by weight parts, and carry out airflow pulverization to obtain mixture a;

[0062] (2) Cut saffron, dianthus superbus, clove, red clover root and oregano leaves by weight parts, wash, dry and mix evenly. Spread the mixture above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b;

[0063] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0064] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong rhizome, saffron, dianthus superbus, clove, red clover root and oregano leaves is 1:5:1:1:5:1:1:1:1.

[0065] Furthermore, in step (2), the volume ratio of ethanol, dichloromethane and N,N-dimethylformamide is 1:5:5.

[0066] Comparative Example 2:

[0067] A preparation method of a Mongolian medicine for treating rhinitis, comprising the following steps:

[0068] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong rhizome by weight parts, and carry out ultrasonic pulverization to obtain mixture a;

[0069] (2) Cut saffron, dianthus superbus, clove, red clover root and oregano leaves by weight parts, wash, dry and mix evenly. Spread the mixture above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b;

[0070] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0071] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong rhizome, saffron, dianthus superbus, clove, red clover root and oregano leaves is 1:5:1:1:5:1:1:1:1.

[0072] Furthermore, in step (2), the volume ratio of ethanol, dichloromethane and N,N-dimethylformamide is 1:5:5.

[0073] Comparative Example 3:

[0074] A preparation method of a Mongolian medicine for treating rhinitis, comprising the following steps:

[0075] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong rhizome by weight parts and carry out high-energy ball milling to obtain mixture a;

[0076] (2) Cut saffron, dianthus superbus, clove, red clover root and oregano leaf by weight parts, wash, dry and mix evenly. Spread the mixture above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain condensate, and dry the condensate in an oven to obtain mixture b;

[0077] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0078] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong rhizome, saffron, dianthus superbus, clove, red clover root and oregano leaf is 1:5:1:1:5:1:1:1:1.

[0079] Furthermore, in step (1), the rotation speed of the high-energy ball milling is 350 r / min and the high-energy ball milling time is 2 h.

[0080] Furthermore, in step (2), the volume ratio of ethanol to dichloromethane is 1:5.

[0081] Comparative Example 4:

[0082] A preparation method of a Mongolian medicine for treating rhinitis, comprising the following steps:

[0083] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong rhizome by weight parts and carry out high-energy ball milling to obtain mixture a;

[0084] (2) Cut saffron, dianthus superbus, clove, red clover root and oregano leaf by weight parts, wash, dry and mix evenly. Spread the mixture above the solvent for fumigation. The solvent is an ethanol solution. Condense and collect the fumigation liquid obtained by volatilization to obtain condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b;

[0085] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0086] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong rhizome, saffron, dianthus superbus, clove, red clover root and oregano leaf is 1:5:1:1:5:1:1:1:1.

[0087] Furthermore, in step (1), the rotation speed of the high-energy ball milling is 350 r / min and the high-energy ball milling time is 2 h.

[0088] Comparative Example 5:

[0089] A preparation method of a Mongolian medicine for treating rhinitis, comprising the following steps:

[0090] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong by weight and carry out high-energy ball milling to obtain mixture a;

[0091] (2) Cut crocus sativus, dianthus superbus, cloves and origanum vulgare leaves by weight, wash, dry and mix them evenly. Spread the mixture above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b;

[0092] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0093] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong, crocus sativus, dianthus superbus, cloves and origanum vulgare leaves is 1:5:1:1:5:1:1:1.

[0094] Further, in step (1), the rotation speed of the high-energy ball milling is 350 r / min, and the high-energy ball milling time is 2 h.

[0095] Further, in step (2), the volume ratio of ethanol, dichloromethane and N,N-dimethylformamide is 1:5:5.

[0096] Comparative Example 6:

[0097] A preparation method of a Mongolian medicine for treating rhinitis, comprising the following steps:

[0098] (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong by weight and carry out high-energy ball milling to obtain mixture a;

[0099] (2) Cut crocus sativus, dianthus superbus, cloves and red clover root by weight, wash, dry and mix them evenly. Spread the mixture above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b;

[0100] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0101] In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong, crocus sativus, dianthus superbus, cloves and red clover root is 1:5:1:1:5:1:1:1.

[0102] Further, in step (1), the rotation speed of the high-energy ball milling is 350 r / min, and the high-energy ball milling time is 2 h.

[0103] Further, in step (2), the volume ratio of ethanol, dichloromethane, and N,N-dimethylformamide is 1:5:5.

[0104] Comparative Example 7:

[0105] A preparation method of a Mongolian medicine for treating rhinitis includes the following steps:

[0106] (1) Mix buffalo horn, bezoar, and chuanxiong by weight and perform high-energy ball milling to obtain mixture a;

[0107] (2) Cut crocus sativus, dianthus superbus, clove, red clover root, and oregano leaf by weight, wash, dry, and mix them evenly. Spread the mixture above the solvent for fumigation. The solvent is a mixed solution of ethanol, dichloromethane, and N,N-dimethylformamide. Condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and perform vacuum freeze-drying on the condensate to obtain mixture b;

[0108] (3) Mix mixture a and mixture b evenly to obtain the Mongolian medicine for treating rhinitis.

[0109] In the preparation method, the weight ratio of buffalo horn, bezoar, chuanxiong, crocus sativus, dianthus superbus, clove, red clover root, and oregano leaf is 1:5:1:5:1:1:1:1.

[0110] Further, in step (1), the rotation speed of the high-energy ball milling is 350 r / min, and the high-energy ball milling time is 2 h.

[0111] Further, in step (2), the volume ratio of ethanol, dichloromethane, and N,N-dimethylformamide is 1:5:5.

[0112] Application Example: Clinical Therapeutic Effect

[0113] There were 981 clinical patients with allergic rhinitis, including 489 males and 492 females. The oldest patient was 78 years old, the youngest was 8 years old. The longest disease course was 7 years, and the shortest was 15 days. The shortest observation time was 1 week, and the longest was 2 years. The patients with allergic rhinitis were randomly divided into 2 treatment groups and 7 control groups. The 2 treatment groups and 7 control groups were respectively given the Mongolian medicines prepared in Examples 1-2 and Comparative Examples 1-7. Each group in the treatment group and the control group had 109 people. The gender, age, disease course, etc. of each group were statistically processed, and the differences were not significant, showing comparability.

[0114] Usage and Dosage: The dosage for treating group 1 is to take the Mongolian medicine prepared in Example 1, 1 dose each time, with warm water, twice a day; the dosage for treating group 2 is to take the Mongolian medicine prepared in Example 2, 1 dose each time, with warm water, twice a day; the control groups 1-7 took the Mongolian medicine prepared in comparative examples 1-7 with warm water respectively, 1 dose each time, twice a day for each group; 7 consecutive days is one course of treatment, and a total of 3 courses of treatment are given.

[0115] According to the diagnostic criteria and efficacy evaluation criteria of allergic rhinitis of the Chinese Medical Association's Otolaryngology Branch: perennial onset, with three main clinical manifestations of sneezing (more than 3 times in a row each time), runny nose and nasal mucosal swelling, cumulative onset days of more than 6 months within 1 year, cumulative onset time of more than 30 minutes within 1 day, at least 1 year of onset, and scoring of 6 points or more.

[0116] According to the diagnostic criteria and efficacy evaluation criteria of allergic rhinitis of the Chinese Medical Association's Otolaryngology Branch: Based on the sum of the scores of symptoms and signs before and after treatment, the percentage of improvement is evaluated according to the following formula to evaluate the efficacy of perennial allergic rhinitis: (total score before treatment - total score after treatment) ÷ total score before treatment × 100%. ≥51% is markedly effective; 50%-21% is effective; ≤20% is ineffective.

[0117] Table 1 Total effective rate and relapse rate of clinical treatment of the Chinese medicine composition of the present invention (%)

[0118] Total number of cases Marked effect Effective Ineffective Total effective rate % Recurrence Recurrence rate % Treatment group 1 109 98 5 6 94.5 2 1.8 Treatment group 2 109 95 9 5 95.4 5 4.6 Control group 1 109 46 11 52 52.3 45 41.3 Control group 2 109 51 9 49 55.0 41 37.6 Control group 3 109 63 15 31 71.6 29 26.6 Control group 4 109 67 19 23 78.9 25 22.9 Control group 5 109 76 19 14 87.2 18 16.5 Control group 6 109 73 7 29 73.3 18 16.5 Control group 7 109 77 6 26 76.1 21 19.3

[0119] As can be seen from Table 1, by comparing treatment group 1 and control group 1-2, it can be seen that the high-energy ball milling of the mixture of buffalo horn, bezoar, sea buckthorn root and Chuanxiong can fully release the active ingredients in buffalo horn, bezoar, sea buckthorn root and Chuanxiong, compared with gas crushing and ultrasonic crushing, and improve the chemical reactivity of the medicinal materials. At the same time, each active ingredient reacts and bonds under the conditions of high-energy ball milling, and obtains Mongolian medicine active ingredients that can effectively treat allergic rhinitis. In addition, the applicant also found that not all medicinal materials can be subjected to high-energy ball milling to improve the efficacy of Mongolian medicine in treating allergic rhinitis. Selecting specific buffalo horn, bezoar, sea buckthorn root and Chuanxiong for high-energy ball milling can make the medicinal materials better exert their medicinal properties compared with other medicinal materials and other mixing methods, and thus can better treat allergic rhinitis.

[0120] Comparing treatment group 1 and control group 3, it can be seen that vacuum freeze-drying was used to dry the fumigation products. Compared with other drying methods, vacuum freeze-drying can better maintain the activity of the extracted active ingredients and better exert the efficacy of the active ingredients in treating allergic rhinitis.

[0121] Comparing Treatment Group 1 and Control Group 4, it can be seen that when using a mixed solvent to fumigate the mixture of saffron, dianthus superbus, clove, red clover root and oregano leaf, and collecting the fumigation liquid after fumigating the mixture by condensation, compared with a single solvent, the selection of the mixed solvent in the present invention can extract the active ingredients for treating allergic rhinitis in medicinal materials more efficiently. By means of the mixed solvent and fumigation, the active ingredients in the mixed medicinal materials are extracted, the purity of the active ingredients for treating allergic rhinitis in Mongolian medicine is improved, and further the efficiency of Mongolian medicine in treating allergic rhinitis is improved. At the same time, the safety of Mongolian medicine in treating allergic rhinitis is improved, and the occurrence of drug side effects is reduced.

[0122] Comparing Treatment Group 1 and Control Groups 5-7, it can be seen that the seabuckthorn root after high-energy ball milling, the oregano leaf fumigated with a mixed solvent, and the red clover root fumigated with a mixed solvent can bring good curative effects for treating allergic rhinitis with these active components.

[0123] The applicant declares that the present invention uses the above embodiments to illustrate the technical solution of the present invention, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvement to the present invention, equivalent measures for each raw material of the product of the present invention, addition of auxiliary components, selection of specific methods, etc. all fall within the protection scope and public scope of the present invention.

[0124] The above content is only an example and illustration of the present invention. Those skilled in the art of the present technology can make various modifications or supplements to the described specific embodiments or use similar methods for substitution, as long as they do not deviate from the invention or exceed the scope defined by the claims of the present invention, they should fall within the protection scope of the present invention.

[0125] In addition, it should be noted that in the above specific embodiments, the various specific technical features described can be combined in any suitable way without conflict. To avoid unnecessary repetition, the present invention will not separately describe various possible combination methods.

Claims

1. Preparation method of Mongolian medicine for treating rhinitis, comprising the following steps: (1) Mix buffalo horn, bezoar, seabuckthorn root and chuanxiong according to parts by weight and carry out high-energy ball milling to obtain mixture a; (2) Cut crocus sativus, dianthus superbus, clove, red clover root and oregano leaves according to parts by weight, wash, dry and mix evenly, lay the mixture flat above the solvent for fumigation, the solvent is a mixed solution of ethanol, dichloromethane and N,N-dimethylformamide, condense and collect the fumigation liquid obtained by volatilization to obtain a condensate, and carry out vacuum freeze-drying on the condensate to obtain mixture b; (3) Mix mixture a and mixture b evenly to obtain Mongolian medicine for treating rhinitis.

2. The preparation method according to claim 1, characterized in that, In step (1), the rotation speed of high-energy ball milling is 350-400 r / min, and the high-energy ball milling time is 2-2.5 h.

3. The preparation method according to claim 1, characterized in that, In the preparation method, the weight ratio of buffalo horn, bezoar, seabuckthorn root, chuanxiong, crocus sativus, dianthus superbus, clove, red clover root and oregano leaves is 1-3:5-6:1-1.5:1-3:5-6:1-3:1-3:1-3:1-3.

4. The preparation method according to claim 1, characterized in that, In step (2), the volume ratio of ethanol, dichloromethane and N,N-dimethylformamide is 1:(5-10):(5-10).

5. The preparation method according to claim 1, characterized in that, In step (2), the drying temperature is 80-120 °C, and the drying time is 3-6 h.

6. A Mongolian medicine for treating rhinitis, characterized in that, The Mongolian medicine is prepared by the preparation method according to any one of claims 1-5.

7. Use of the Mongolian medicine for treating rhinitis according to claim 6, characterized in that, The Mongolian medicine is used for treating allergic rhinitis.