Anti-aging composition containing TGF-B1 stem cell extract

Abstract

The invention relates to the technical field of biomedical engineering, and discloses an anti-aging composition containing a TGF-B1 stem cell extract, and the composition comprises the following components in parts by mass: 8-9 parts of TGF-B1 stem cell extract; 0.5 to 0.9 part of a grape seed extract; 1 to 1.2 parts of calystegia hederacea extract; 0.8 to 1.3 parts of a mugwort extract; 1.8 to 2.1 parts of an aronia extract; 0.5 to 0.8 part of potentilla chinensis extract; 2.1 to 2.5 parts of a sow thistle extract; 3-5 parts of a ginseng extract; 20 to 30 parts of an aloe extract; 0.012 to 0.022 part of a clove extract; 0.01 to 0.019 part of a ginkgo leaf extract; the preparation method comprises the following steps: dividing the components into four types A, B, C and D, mixing under different conditions, sequentially adding into a homogenizer, homogenizing to obtain the composition, filtering, sterilizing, packaging, and storing in an environment of-5 to 5 DEG C. The composition can activate cell viability and slow down skin aging from the source; and the components in the formula have high safety and wide compatibility at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical engineering, and specifically to an anti-aging composition containing TGF-B1 stem cell extract. Background Art

[0002] In the field of anti-aging, existing anti-aging formulations have many limitations. On the one hand, these formulations often fail to activate cell activity at the root, making it difficult to deeply intervene in and effectively reverse the aging process. The reduction of cell activity is one of the important factors leading to human aging. With the increase of age or the influence of the external environment, the metabolic, proliferative, and self-repair abilities of cells gradually weaken, and most of the existing formulations can only relieve the appearance of aging, such as temporarily improving the water content of the skin or reducing surface wrinkles, but cannot truly awaken the vitality of cells and restore them to a young and healthy state. On the other hand, most anti-aging products on the market are mainly composed of chemical components. Although these chemical components may have certain anti-aging effects in some aspects, long-term use may bring potential safety hazards. Chemical synthetic substances may cause adverse reactions such as skin allergies and irritations, which have an adverse impact on human health. Moreover, due to the complexity and singularity of chemical components, it is difficult to balance the compatibility of the product while ensuring safety, and it cannot meet the needs of different skin types and individuals. In this context, finding an anti-aging ingredient that can activate cell activity at the root and has high safety and wide compatibility has become an urgent need in the anti-aging research field. The emergence of TGF-B1 stem cell extract brings new hope and direction for solving these problems. Summary of the Invention

[0003] (1) Technical Problems to be Solved

[0004] In view of the deficiencies of the prior art, the present invention provides an anti-aging composition containing TGF-B1 stem cell extract, which has the advantages of activating cell activity at the root and having high safety and wide compatibility of components, and solves the problems that existing formulations cannot activate the vitality of cells and it is difficult to balance the compatibility of the product while ensuring safety.

[0005] (2) Technical Solutions

[0006] To achieve the above object, the present invention provides the following technical solution: An anti-aging composition containing TGF-B1 stem cell extract, and the components and their mass fraction ranges of the composition are as follows: 8-9 parts of TGF-B1 stem cell extract; 0.5-0.9 part of grape seed extract; 1-1.2 parts of Calystegia hederacea extract; 0.8-1.3 parts of Artemisia lavandulaefolia extract; 1.8-2.1 parts of Aronia melanocarpa extract; 0.5-0.8 part of Potentilla chinensis extract; 2.1-2.5 parts of Sonchus oleraceus extract; 3-5 parts of ginseng extract; 20-30 parts of aloe extract; 0.012-0.022 part of clove extract; 0.011-0.019 part of ginkgo leaf extract; 0.04-0.06 part of Chinese prickly ash extract.

[0007] Preferably, the components and their mass fractions of the composition are as follows: 9 parts of TGF-B1 stem cell extract; 0.6 part of grape seed extract; 1 part of Calystegia hederacea extract; 0.9 part of Artemisia lavandulaefolia extract; 2.1 parts of Aronia melanocarpa extract; 0.65 part of Potentilla chinensis extract; 2.2 parts of Sonchus oleraceus extract; 3.5 parts of ginseng extract; 22 parts of aloe extract; 0.015 part of clove extract; 0.016 part of ginkgo leaf extract; 0.05 part of Chinese prickly ash extract.

[0008] Preferably, the components and their mass fractions of the composition are as follows: 9 parts of TGF-B1 stem cell extract; 0.5 part of grape seed extract; 1.2 parts of Calystegia hederacea extract; 1.3 parts of Artemisia lavandulaefolia extract; 1.8 parts of Aronia melanocarpa extract; 0.5 part of Potentilla chinensis extract; 2.4 parts of Sonchus oleraceus extract; 4.5 parts of ginseng extract; 30 parts of aloe extract; 0.022 part of clove extract; 0.013 part of ginkgo leaf extract; 0.04 part of Chinese prickly ash extract.

[0009] Preferably, the components and their mass fractions of the composition are as follows: 8.6 parts of TGF-B1 stem cell extract; 0.8 part of grape seed extract; 1.1 parts of Calystegia hederacea extract; 0.95 part of Artemisia lavandulaefolia extract; 1.9 parts of Aronia melanocarpa extract; 0.55 part of Potentilla chinensis extract; 2.3 parts of Sonchus oleraceus extract; 4.5 parts of ginseng extract; 26 parts of aloe extract; 0.019 part of clove extract; 0.013 part of ginkgo leaf extract; 0.046 part of Chinese prickly ash extract.

[0010] Preferably, the composition is composed of a skin conditioner, an antioxidant, an astringent, an anti-inflammatory agent and a natural preservative.

[0011] Preferably, the skin conditioner is composed of TGF-B1 stem cell extract, ginseng extract and aloe extract, the antioxidant is composed of grape seed extract and Sonchus oleraceus extract, the astringent is composed of Potentilla chinensis extract, the anti-inflammatory agent is composed of Artemisia lavandulaefolia extract and clove extract, and the natural preservative is composed of ginkgo leaf extract and Chinese prickly ash extract.

[0012] Preferably, an anti-aging composition containing TGF-B1 stem cell extract, and the preparation process of the composition includes the following steps:

[0013] S1. Component extraction and disinfection: Each plant in the formula is extracted by the supercritical carbon dioxide extraction method to obtain grape seed extract, Calystegia hederacea extract, Artemisia lavandulaefolia extract, elderberry extract, Potentilla chinensis extract, Sonchus oleraceus extract, ginseng extract, aloe extract, clove extract, ginkgo leaf extract and Chinese prickly ash extract, and the TGF-B1 stem cell extract is sterilized.

[0014] S2. Preparation of component mass fractions: Weigh the TGF-B1 stem cell extract, grape seed extract, Calystegia hederacea extract, Artemisia lavandulaefolia extract, elderberry extract, Potentilla chinensis extract, Sonchus oleraceus extract, ginseng extract, aloe extract, clove extract, ginkgo leaf extract and Chinese prickly ash extract according to the mass fractions specified in the formula.

[0015] S3. Classification: Divide the components in S2 into four categories A, B, C, and D, and set four different conditions for mixing.

[0016] S4. Homogenization: Add the components after mixing the four categories A, B, C, and D into a homogenizer in turn, and perform homogenization treatment at a rotation speed of 2000 - 3000 rpm for 15 - 20 minutes to form a composition.

[0017] S5. Filtration: After homogenization, filter the composition 1 - 3 times with filter paper under sterile conditions.

[0018] S6. Sterilization and packaging: Sterilize the filtered composition under low temperature conditions with an ultraviolet wavelength of 253.7 nm, a duration of 30 - 45 minutes, and a temperature of 20 - 25 °C, and then sub-pack the sterilized composition into sterile containers.

[0019] S7. Preservation: Place the sterile container containing the composition in an environment with a temperature of -5 to 5 °C for preservation.

[0020] Preferably, category A consists of TGF-B1 stem cell extract, ginseng extract and aloe extract. In an environment with a temperature of 20 - 25 °C, mix the TGF-B1 stem cell extract with the aloe extract, stir evenly, then add the ginseng extract, and continue stirring for 10 - 15 minutes.

[0021] Preferably, category B consists of grape seed extract, Calystegia hederacea extract, Artemisia lavandulaefolia extract and elderberry extract. Under the temperature control condition of 35 - 40 °C, mix the grape seed extract with the Calystegia hederacea extract, stir for 5 - 6 minutes, then add the Artemisia lavandulaefolia extract and elderberry extract, and continue stirring for 10 - 15 minutes.

[0022] Preferably, the class C is composed of Potentilla chinensis extract and Sonchus oleraceus extract, and under the environmental condition of 45-50 °C, the Potentilla chinensis extract and the Sonchus oleraceus extract are mixed and stirred for 5-10 minutes. The class D is composed of Syzygium aromaticum extract, Ginkgo biloba extract and Zanthoxylum bungeanum extract, and under the low temperature condition of 15-20 °C, the Syzygium aromaticum extract, the Ginkgo biloba extract and the Zanthoxylum bungeanum extract are mixed and stirred for 5-10 minutes.

[0023] Compared with the prior art, the present invention provides an anti-aging composition containing TGF-B1 stem cell extract, and has the following beneficial effects:

[0024] 1. By adding TGF-B1 stem cell extract and ginseng extract to the formula, the TGF-B1 stem cell extract can directly activate cell activity, promote deep repair and anti-aging, and at the same time, when combined with ginseng extract, it can provide nutrition for cells, enhance cell metabolism and skin elasticity.

[0025] 2. By adding Calystegia hederacea Wall. extract, Aronia melanocarpa extract and Zanthoxylum bungeanum extract to the formula, the prepared composition has a synergistic effect when used, so as to assist the TGF-B1 stem cell extract to enhance the antioxidant and anti-inflammatory capabilities, promote skin microcirculation, and the added Zanthoxylum bungeanum extract can be used as a natural preservative, which can replace chemical preservatives, and while improving the safety of the product, it also has antibacterial and metabolic promoting effects, solving the problem that it is difficult for existing formulas to balance the compatibility of products while ensuring safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a process step diagram of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0028] Please refer to Figure 1, An anti-aging composition containing TGF-B1 stem cell extract, the components and their mass fraction ranges of the composition are: 8-9 parts of TGF-B1 stem cell extract; 0.5-0.9 part of grape seed extract; 1-1.2 parts of Calystegia hederacea extract; 0.8-1.3 parts of Artemisia lavandulaefolia extract; 1.8-2.1 parts of black chokeberry extract; 0.5-0.8 part of Potentilla chinensis extract; 2.1-2.5 parts of Sonchus oleraceus extract; 3-5 parts of ginseng extract; 20-30 parts of aloe extract; 0.012-0.022 part of clove extract; 0.011-0.019 part of ginkgo leaf extract; 0.04-0.06 part of Chinese prickly ash extract.

[0029] Specifically, the components and their mass fractions of the composition are: 8.6 parts of TGF-B1 stem cell extract; 0.8 part of grape seed extract; 1.1 parts of Calystegia hederacea extract; 0.95 part of Artemisia lavandulaefolia extract; 1.9 parts of black chokeberry extract; 0.55 part of Potentilla chinensis extract; 2.3 parts of Sonchus oleraceus extract; 4.5 parts of ginseng extract; 26 parts of aloe extract; 0.019 part of clove extract; 0.013 part of ginkgo leaf extract; 0.046 part of Chinese prickly ash extract.

[0030] The functions corresponding to the above components are shown in Table 1:

[0031] Table 1

[0032]

[0033]

[0034] Specifically, the composition consists of a skin conditioner, an antioxidant, an astringent, an anti-inflammatory agent and a natural preservative.

[0035] Specifically, the skin conditioner consists of TGF-B1 stem cell extract, ginseng extract and aloe extract, the antioxidant consists of grape seed extract and Sonchus oleraceus extract, the astringent consists of Potentilla chinensis extract, the anti-inflammatory agent consists of Artemisia lavandulaefolia extract and clove extract, and the natural preservative consists of ginkgo leaf extract and Chinese prickly ash extract.

[0036] Specifically, for the preparation process of an anti-aging composition containing TGF-B1 stem cell extract, the following steps are included:

[0037] S1. Component extraction and disinfection: Each plant in the formula is extracted using supercritical carbon dioxide extraction method to obtain grape seed extract, Calystegia hederacea Wall. extract, Artemisia lavandulaefolia DC. extract, elderberry extract, Potentilla chinensis Ser. extract, Sonchus oleraceus L. extract, Panax ginseng C. A. Mey. extract, Aloe vera extract, Syzygium aromaticum (L.) Merr. & Perry extract, Ginkgo biloba extract and Zanthoxylum bungeanum Maxim. extract, ensuring the integrity of the purity of the raw materials while retaining the biological activity of the extracts. Sterile treatment is carried out on the TGF-B1 stem cell extract to ensure that its biological activity is not damaged;

[0038] S2. Preparation of component mass fractions: Weigh the TGF-B1 stem cell extract, grape seed extract, Calystegia hederacea Wall. extract, Artemisia lavandulaefolia DC. extract, elderberry extract, Potentilla chinensis Ser. extract, Sonchus oleraceus L. extract, Panax ginseng C. A. Mey. extract, Aloe vera extract, Syzygium aromaticum (L.) Merr. & Perry extract, Ginkgo biloba extract and Zanthoxylum bungeanum Maxim. extract according to the mass fractions specified in the formula to ensure the accuracy of the component ratio;

[0039] S3. Classification: Divide the components in S2 into four categories, A, B, C, and D, and set four different conditions for mixing;

[0040] S4. Homogenization: Add the components after mixing the four categories A, B, C, and D into the homogenizer in sequence, and carry out homogenization treatment at a rotation speed of 2000 - 3000 rpm for 15 - 20 minutes to form a composition, ensuring that all components are fully mixed to form a uniform solution;

[0041] S5. Filtration: After homogenization, filter the composition 1 - 3 times using filter paper under sterile conditions to remove possible impurities and ensure the purity of the final product;

[0042] S6. Sterilization and packaging: Carry out low-temperature sterilization treatment on the filtered composition under the conditions of an ultraviolet wavelength of 253.7 nm, a duration of 30 - 45 minutes, and a temperature of 20 - 25 °C. The sterilized composition is sub-packed into sterile containers to ensure the high safety of the product;

[0043] S7. Preservation: Place the sterile container containing the composition in an environment with a temperature of -5 to 5 °C for preservation, seal it for preservation, avoid light and high temperature, and ensure the stability of the components.

[0044] Advantages: The synergistic effect of the TGF-B1 stem cell extract and various plant extracts can effectively activate the self-repair and regeneration ability of cells, delay cell aging. All components have undergone strict sterile treatment and low-temperature extraction to ensure that the product is non-irritating to the skin and suitable for long-term use. The components in the composition are scientifically proportioned and can be compatible with a variety of skin types, suitable for people of different ages and skin types, solving the problem that existing formulas are difficult to balance safety and compatibility.

[0045] Specifically, Class A consists of TGF-B1 stem cell extract, ginseng extract, and aloe extract. In an environment with a temperature of 20 - 25°C, the TGF-B1 stem cell extract is mixed with the aloe extract, stirred evenly, and then the ginseng extract is added, and stirring continues for 10 - 15 minutes to ensure thorough mixing.

[0046] Specifically, Class B consists of grape seed extract, Calystegia hederacea extract, Artemisia lavandulaefolia extract, and chokeberry extract. Under temperature control conditions of 35 - 40°C, the grape seed extract is mixed with the Calystegia hederacea extract, stirred for 5 - 6 minutes, then the Artemisia lavandulaefolia extract and chokeberry extract are added, and stirring continues for 10 - 15 minutes to ensure uniform dispersion of the components.

[0047] Specifically, Class C consists of Potentilla chinensis extract and Sonchus oleraceus extract. Under environmental conditions with a temperature of 45 - 50°C, the Potentilla chinensis extract is mixed with the Sonchus oleraceus extract, stirred for 5 - 10 minutes to ensure thorough fusion of the components. Class D consists of clove extract, ginkgo biloba extract, and Chinese prickly ash extract. Under low temperature conditions of 15 - 20°C, the clove extract, ginkgo biloba extract, and Chinese prickly ash extract are mixed, stirred for 5 - 10 minutes to ensure uniformity of the components.

[0048] Example 1

[0049] Specifically, the components and their mass parts of the composition are: 9 parts of TGF-B1 stem cell extract; 0.6 part of grape seed extract; 1 part of Calystegia hederacea extract; 0.9 part of Artemisia lavandulaefolia extract; 2.1 parts of chokeberry extract; 0.65 part of Potentilla chinensis extract; 2.2 parts of Sonchus oleraceus extract; 3.5 parts of ginseng extract; 22 parts of aloe extract; 0.015 part of clove extract; 0.016 part of ginkgo biloba extract; 0.05 part of Chinese prickly ash extract.

[0050] Comparative Example 1

[0051] Specifically, the components and their mass parts of the composition are: 9 parts of FGF (fibroblast growth factor) extract; 0.6 part of grape seed extract; 1 part of Calystegia hederacea extract; 0.9 part of Artemisia lavandulaefolia extract; 2.1 parts of chokeberry extract; 0.65 part of Potentilla chinensis extract; 2.2 parts of Sonchus oleraceus extract; 22 parts of aloe extract; 0.015 part of clove extract; 0.016 part of ginkgo biloba extract; 0.05 part of Chinese prickly ash extract (replacing the TGF-B1 stem cell extract in Example 1 with FGF (fibroblast growth factor) extract), and the ginseng extract is deleted.

[0052] The performance tests were carried out on the composition products prepared in Example 1 and Comparative Example 1, and the test data are shown in Table 2 below:

[0053] Table 2

[0054]

[0055]

[0056] Analysis based on Table 2 shows that:

[0057] In Example 1, the TGF-β1 stem cell extract can directly activate cell activity, promote deep repair and anti-aging. At the same time, when combined with ginseng extract, it can provide nutrition for cells, enhance cell metabolism and skin elasticity. In Comparative Example 1, the FGF (fibroblast growth factor) extract stimulates fibroblasts to produce collagen, but has a relatively large ability to activate the overall cell activity. And without ginseng extract, it will reduce the cell nutritional support and lower the overall anti-aging effect.

[0058] Example 2

[0059] Specifically, the components and their mass parts of the composition are: 9 parts of TGF-β1 stem cell extract; 0.5 part of grape seed extract; 1.2 parts of Calystegia hederacea extract; 1.3 parts of Artemisia lavandulaefolia extract; 1.8 parts of elderberry extract; 0.5 part of Potentilla chinensis extract; 2.4 parts of Sonchus oleraceus extract; 4.5 parts of ginseng extract; 30 parts of aloe extract; 0.022 part of clove extract; 0.013 part of ginkgo leaf extract; 0.04 part of Zanthoxylum bungeanum extract.

[0060] Comparative Example 2

[0061] Specifically, the components and their mass parts of the composition are: 9 parts of TGF-β1 stem cell extract; 0.5 part of grape seed extract; 1.3 parts of Artemisia lavandulaefolia extract; 0.5 part of Potentilla chinensis extract; 2.4 parts of Sonchus oleraceus extract; 4.5 parts of ginseng extract; 30 parts of aloe extract; 0.022 part of clove extract; 0.013 part of ginkgo leaf extract; 0.02 - 0.025 part of methylparaben (replacing Calystegia hederacea extract, elderberry extract and Zanthoxylum bungeanum extract with methylparaben).

[0062] Perform performance tests on the composition products prepared from Example 2 and Comparative Example 2, and the test data are shown in Table 3 below:

[0063] Table 3

[0064]

[0065] The following information is obtained from the analysis of Table 3:

[0066] In Example 2, the combination of Calystegia hederacea Wall. extract and Aronia melanocarpa extract synergistically enhances the antioxidant and anti-inflammatory capabilities of the resulting composition, thereby assisting TGF-B1 stem cell extract to promote skin microcirculation. The added Zanthoxylum bungeanum Maxim. extract can serve as a natural preservative, replacing chemical preservatives to improve product safety, while also having antibacterial and metabolism-promoting effects. In the formulation of Comparative Example 2, Calystegia hederacea Wall. extract, Aronia melanocarpa extract, and Zanthoxylum bungeanum Maxim. extract are not included, resulting in a decrease in the antioxidant and anti-inflammatory capabilities of the prepared composition. Chemical preservatives must be added additionally to improve compatibility. However, chemical preservatives have low safety and cannot reduce the irritation of the composition to the skin.

[0067] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An anti-aging composition containing TGF-B1 stem cell extract, characterized in that, The components of the composition and their parts by mass range are as follows: 8 - 9 parts of TGF - B1 stem cell extract; 0.5 - 0.9 part of grape seed extract; 1 - 1.2 parts of Calystegia hederacea extract; 0.8 - 1.3 parts of Artemisia lavandulaefolia extract; 1.8 - 2.1 parts of Aronia melanocarpa extract; 0.5 - 0.8 part of Potentilla chinensis extract; 2.1 - 2.5 parts of Sonchus oleraceus extract; 3 - 5 parts of ginseng extract; 20 - 30 parts of aloe extract; 0.012 - 0.022 part of clove extract; 0.011 - 0.019 part of ginkgo leaf extract; 0.04 - 0.06 part of Chinese prickly ash extract.

2. The anti-aging composition containing TGF-β1 stem cell extract according to claim 1, wherein: The components of the composition and their parts by mass are as follows: 9 parts of TGF - B1 stem cell extract; 0.6 part of grape seed extract; 1 part of Calystegia hederacea extract; 0.9 part of Artemisia lavandulaefolia extract; 2.1 parts of Aronia melanocarpa extract; 0.65 part of Potentilla chinensis extract; 2.2 parts of Sonchus oleraceus extract; 3.5 parts of ginseng extract; 22 parts of aloe extract; 0.015 part of clove extract; 0.016 part of ginkgo leaf extract; 0.05 part of Chinese prickly ash extract.

3. The anti-aging composition containing TGF-β1 stem cell extract according to claim 1, wherein: The components of the composition and their parts by mass are as follows: 9 parts of TGF - B1 stem cell extract; 0.5 part of grape seed extract; 1.2 parts of Calystegia hederacea extract; 1.3 parts of Artemisia lavandulaefolia extract; 1.8 parts of Aronia melanocarpa extract; 0.5 part of Potentilla chinensis extract; 2.4 parts of Sonchus oleraceus extract; 4.5 parts of ginseng extract; 30 parts of aloe extract; 0.022 part of clove extract; 0.013 part of ginkgo leaf extract; 0.04 part of Chinese prickly ash extract.

4. An anti-aging composition containing TGF-β1 stem cell extract according to claim 1, characterized in that: The components of the composition and their parts by mass are as follows: 8.6 parts of TGF - B1 stem cell extract; 0.8 part of grape seed extract; 1.1 parts of Calystegia hederacea extract; 0.95 part of Artemisia lavandulaefolia extract; 1.9 parts of Aronia melanocarpa extract; 0.55 part of Potentilla chinensis extract; 2.3 parts of Sonchus oleraceus extract; 4.5 parts of ginseng extract; 26 parts of aloe extract; 0.019 part of clove extract; 0.013 part of ginkgo leaf extract; 0.046 part of Chinese prickly ash extract.

5. The anti-aging composition containing TGF-β1 stem cell extract according to claim 1, characterized in that: The composition is composed of a skin conditioner, an antioxidant, an astringent, an anti - inflammatory agent and a natural preservative.

6. The anti-aging composition containing TGF-β1 stem cell extract according to claim 5, wherein: The skin conditioner is composed of TGF - B1 stem cell extract, ginseng extract and aloe extract; the antioxidant is composed of grape seed extract and Sonchus oleraceus extract; the astringent is composed of Potentilla chinensis extract; the anti - inflammatory agent is composed of Artemisia lavandulaefolia extract and clove extract; the natural preservative is composed of ginkgo leaf extract and Chinese prickly ash extract.

7. An anti-aging composition containing TGF-β1 stem cell extract according to claim 1, characterized in that, The preparation process of the composition includes the following steps: S1. Component extraction and disinfection: Each plant in the formula is extracted by the supercritical carbon dioxide extraction method to obtain grape seed extract, Calystegia hederacea extract, Artemisia lavandulaefolia extract, Aronia melanocarpa extract, Potentilla chinensis extract, Sonchus oleraceus extract, ginseng extract, aloe extract, clove extract, ginkgo leaf extract and Chinese prickly ash extract, and the TGF - B1 stem cell extract is subjected to aseptic treatment. S2. Preparation of component mass fractions: Weigh TGF-B1 stem cell extract, grape seed extract, Calystegia hederacea extract, Artemisia lavandulaefolia extract, chokeberry extract, Potentilla chinensis extract, Sonchus oleraceus extract, ginseng extract, aloe extract, clove extract, ginkgo leaf extract and Zanthoxylum bungeanum extract according to the mass fractions specified in the formula. S3. Classification: Divide the components in S2 into four categories, A, B, C, and D, and set four different conditions for mixing. S4. Homogenization: Add the components after mixing the four categories A, B, C, and D into the homogenizer in sequence, and carry out homogenization treatment at a rotation speed of 2000 - 3000 rpm for 15 - 20 minutes to form a composition. S5. Filtration: After homogenization, filter the composition 1 - 3 times using filter paper under sterile conditions. S6. Sterilization and packaging: Carry out low-temperature sterilization treatment on the filtered composition under the conditions of an ultraviolet wavelength of 253.7 nm, a duration of 30 - 45 minutes, and a temperature of 20 - 25 °C. After sterilization, sub-pack the composition into sterile containers. S7. Storage: Place the sterile containers containing the composition in an environment with a temperature of -5 to 5 °C for storage.

8. An anti-aging composition containing TGF-β1 stem cell extract according to claim 7, characterized in that: The category A consists of TGF-B1 stem cell extract, ginseng extract and aloe extract. In an environment with a temperature of 20 - 25 °C, mix the TGF-B1 stem cell extract and aloe extract, stir evenly, then add the ginseng extract and continue to stir for 10 - 15 minutes.

9. The anti-aging composition containing TGF-β1 stem cell extract according to claim 7, characterized in that: The category B consists of grape seed extract, Calystegia hederacea extract, Artemisia lavandulaefolia extract and chokeberry extract. Under the temperature control condition of 35 - 40 °C, mix the grape seed extract and Calystegia hederacea extract, stir for 5 - 6 minutes, then add the Artemisia lavandulaefolia extract and chokeberry extract, and continue to stir for 10 - 15 minutes.

10. An anti-aging composition containing TGF-β1 stem cell extract according to claim 7, characterized in that: The category C consists of Potentilla chinensis extract and Sonchus oleraceus extract. Under the environmental condition of a temperature of 45 - 50 °C, mix the Potentilla chinensis extract and Sonchus oleraceus extract and stir for 5 - 10 minutes. The category D consists of clove extract, ginkgo leaf extract and Zanthoxylum bungeanum extract. Under the low-temperature condition of 15 - 20 °C, mix the clove extract, ginkgo leaf extract and Zanthoxylum bungeanum extract and stir for 5 - 10 minutes.

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