Composition with anti-wrinkle and firming effects, cosmetic and preparation method thereof

Abstract

The invention belongs to the technical field of daily chemicals, and particularly relates to a composition with anti-wrinkle and firming effects, a cosmetic and a preparation method thereof, and the composition is prepared from the following components in parts by mass: 4.5 to 5.5 parts of deionized water, 1 to 3 parts of glycerol, 1.5 to 2.5 parts of butanediol, 0.005 to 0.02 part of caprylyl glycol, 0.0008 to 0.002 part of acetyl hexapeptide-8 and 0.2 to 0.4 part of hydroxypropyl tetrahydropyrantriol. According to the anti-wrinkle and firming composition, the cosmetic and the preparation method of the cosmetic, the composition is compounded, acetyl hexapeptide-8 and synapse-related protein SNAP-25 compete for the position in an SNARE compound, so that formation of the compound is interfered, muscle contraction is reduced, wrinkles are reduced, and the anti-wrinkle and firming composition has the advantages that the anti-wrinkle and firming effect is achieved; meanwhile, hydroxypropyl tetrahydropyrantriol in a composition system can influence extracellular matrixes in a three-layer structure of the skin and effectively keep the skin compact and fine, so that skin aging is delayed, the skin care product has the anti-wrinkle and compact effects, and the defects that part of active matter ingredients are unstable and irritate the skin are overcome.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily chemicals, and in particular relates to a composition with anti-wrinkle and firming effects, a cosmetic and a preparation method thereof. Background Art

[0002] As people's living standards continue to improve, after meeting their basic needs for food, clothing, housing and transportation, they are beginning to pay attention to other aspects, such as their appearance. With the development of medicine and technological advancements, the exploration of aging and anti-aging has become a global topic and is constantly deepening. Anti-aging cosmetics are also attracting more and more attention.

[0003] Existing skincare products typically strengthen the skin through long-term repair of the extracellular matrix, or by adding antioxidants to reduce free radical damage, regulate immunity, and enhance the skin's self-protective mechanisms. Alternatively, they incorporate plant oils to improve skin aging, accelerate protein secretion, and prevent aging. Naturally active antioxidants can also effectively prevent pigmentation caused by UV rays and other harmful radiation, thereby improving skin radiance.

[0004] In the concept of skin care products, anti-wrinkle mainly targets already formed static lines (such as nasolabial folds and crow's feet) or dynamic lines (such as forehead wrinkles), and smoothes the skin by promoting collagen regeneration and fading lines. Firming focuses on improving skin sagging, reshaping facial contours (such as sagging apple muscles and blurred jaw lines), and enhancing support by increasing the density of elastic fibers.

[0005] Commonly used anti-wrinkle and firming ingredients such as retinol can easily cause skin irritation and are easily oxidized and degraded, while vitamin C has disadvantages such as instability and easy decomposition.

[0006] Therefore, how to provide a composition with anti-wrinkle and firming effects that can overcome the drawbacks of anti-wrinkle and firming active ingredients such as skin irritation, instability, and easy decomposition is a technical problem that urgently needs to be solved in this field.

[0007] It should be noted that the above information disclosed in this background technology section is only used to understand the background technology of the present application concept, and therefore, the above description is not considered to constitute information of the prior art. Summary of the Invention

[0008] The embodiments of the present disclosure provide at least one composition, cosmetics and preparation method thereof with anti-wrinkle and firming effects.

[0009] In a first aspect, an embodiment of the present disclosure provides a skin care composition comprising the following components in parts by mass: 4.5-5.5 parts of deionized water, 1-3 parts of glycerin, 1.5-2.5 parts of butylene glycol, 0.005-0.02 parts of caprylyl glycol, 0.0008-0.002 parts of acetyl hexapeptide-8, and 0.2-0.4 parts of hydroxypropyl tetrahydropyrantriol.

[0010] In a second aspect, the embodiments of the present disclosure also provide an anti-wrinkle and firming water, comprising the following components: Phase A, comprising: 80-85 parts of deionized water, 4-8 parts of glycerin, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, and 0.01-0.03 parts of xanthan gum; Phase B, comprising: 0.3-0.6 parts of parahydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase C, comprising: 6-10 parts of the skin care composition described above.

[0011] In a third aspect, the embodiments of the present disclosure also provide a method for preparing the cosmetics as described above, comprising the following steps: step S11, pretreatment, i.e., mixing and heating phase A uniformly, heating and stirring phase B until transparent, and the temperature of phase A is greater than the temperature of phase B; step S12, cooling phase A and adding it to phase B and stirring uniformly to obtain an AB mixed phase; step S13, cooling the AB mixed phase, adding phase C and stirring and dispersing uniformly to obtain a mixed phase; step S14, cooling the mixed phase to room temperature to obtain anti-wrinkle firming water.

[0012] In an optional embodiment, in step S11, phase A is heated to 85°C and phase B is heated to 60°C; in step S12, phase A is cooled to 60°C; and in step S13, the AB mixed phase is cooled to 45°C.

[0013] In a fourth aspect, the embodiments of the present disclosure further provide a cosmetic, which is an emulsion, comprising the following components: Phase A, comprising: 70-75 parts of deionized water, 6-10 parts of glycerin, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, 0.01-0.03 parts of xanthan gum, and 0.05-0.2 parts of sodium stearoyl glutamate; Phase B, comprising: 1-2 parts of C14-22 alcohol, 0.3-0.5 parts of C12-20 alkyl glucoside, 0.5-1.5 parts of stearyl alcohol, 1-3 parts of squalane, and 3-5 parts of polydimethylsiloxane; Phase C, comprising: 0.3-0.6 parts of parahydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase D, comprising: 6-10 parts of the skin care composition described above.

[0014] In a fifth aspect, the embodiments of the present disclosure also provide a method for preparing the cosmetics as described above, comprising the following steps: step S21, pretreatment, i.e., mixing phase A evenly and heating, and heating phase C and stirring and dispersing it until transparent; step S22, heating phase B and adding it to phase A, homogenizing to obtain an AB mixed phase; step S23, cooling the AB mixed phase, adding phase D, and homogenizing to obtain an ABD mixed phase; step S24, cooling the ABD mixed phase, adding phase C, stirring and dispersing it evenly to obtain a mixed phase; step S25, continuing to stir the mixed phase and cooling it to room temperature to obtain an anti-wrinkle firming lotion.

[0015] In an optional embodiment, in step S21, phase A is heated to 85°C and phase C is heated to 60°C; in step S22, phase B is heated to 85°C; in step S23, the AB mixed phase is cooled to 60°C; and in step S24, the ABD mixed phase is cooled to 45°C.

[0016] In a sixth aspect, the embodiments of the present disclosure further provide a cosmetic, which is a firming cream, comprising the following components: Phase A, comprising: 65-70 parts of deionized water, 6-10 parts of glycerin, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, 0.01-0.03 parts of xanthan gum, and 0.05-0.2 parts of sodium stearoyl glutamate; Phase B, comprising: 1-3 parts of C14-22 alcohol, 0.4-0.6 parts of C12-20 alkyl glucoside, 3-5 parts of stearyl alcohol, 2-4 parts of squalane, and 3-5 parts of polydimethylsiloxane; Phase C, comprising: 0.3-0.6 parts of parahydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase D, comprising: 6-10 parts of the skin care composition described above.

[0017] In a seventh aspect, the embodiments of the present disclosure also provide a method for preparing the cosmetics as described above, comprising the following steps: step S31, pretreatment, i.e., mixing phase A uniformly and heating, and heating phase C and stirring and dispersing it until transparent; step S32, heating phase B and adding it to phase A, homogenizing to obtain an AB mixed phase; step S33, cooling the AB mixed phase, adding phase D, and homogenizing to obtain an ABD mixed phase; step S34, cooling the ABD mixed phase, adding phase C, stirring and dispersing it uniformly to obtain a mixed phase; step S35, continuing to stir the mixed phase and cooling it to room temperature to obtain an anti-wrinkle firming cream.

[0018] In an optional embodiment, in step S31, phase A is heated to 85°C and phase C is heated to 60°C; in step S32, phase B is heated to 85°C; in step S33, the AB mixed phase is cooled to 60°C; and in step S34, the ABD mixed phase is cooled to 45°C.

[0019] The beneficial effects of the present invention are that the composition, cosmetics and preparation method thereof with anti-wrinkle and firming effects utilize acetyl hexapeptide-8 to compete with the synaptosome-associated protein SNAP-25 for a position in the SNARE complex by compounding the composition, thereby interfering with the formation of the complex, reducing muscle contraction to reduce wrinkles, and at the same time, hydroxypropyl tetrahydropyrantriol in the composition system can affect the extracellular matrix in the three-layer structure of the skin, effectively keeping the skin firm and delicate, thereby delaying skin aging, so that the skin care product has both anti-wrinkle and firming effects, and also avoids the disadvantages of some active ingredients being unstable and irritating to the skin.

[0020] Other features and advantages of the present invention will be described in the following description, and in part will become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention are realized and obtained by the structures particularly pointed out in the description and the drawings.

[0021] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0023] Figure 1 A comparison chart of the moisturizing effects of Example 1 provided in the embodiments of the present disclosure;

[0024] Figure 2 A bar graph showing the relative expression of the eln1 gene according to Example 2 of the present disclosure;

[0025] Figure 3 This is a bar graph of the relative expression level of the col1a1a gene provided in an embodiment of the present disclosure. DETAILED DESCRIPTION

[0026] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0027] As used herein, the phrases "in one embodiment," "according to one embodiment," "in some embodiments," and the like generally refer to the fact that the particular feature, structure, or characteristic following the phrase may be included in at least one embodiment of the present disclosure. Thus, a particular feature, structure, or characteristic may be included in more than one embodiment of the present disclosure, such that these phrases do not necessarily refer to the same embodiment. As used herein, the terms "example," "exemplary," and the like are used to "serve as an example, instance, or illustration." Any implementation, aspect, or design described herein as "example" or "exemplary" is not necessarily to be construed as preferred or advantageous over other implementations, aspects, or designs. Instead, the use of the terms "example," "exemplary," and the like is intended to present concepts in a concrete manner.

[0028] Herein, example embodiments of the present disclosure will be described in more detail with reference to the accompanying drawings. As used herein, expressions such as "at least one of..." when following a list of elements modify the entire list of elements, rather than modifying individual elements in the list. For example, the expression "at least one of a, b, and c" should be understood to include only a, only b, only c, both a and b, both a and c, both b and c, or all of a, b, and c.

[0029] The terms used herein are only used to describe specific exemplary configurations and are not intended to be limiting. As used herein, the singular articles "a", "an" and "the" may also be intended to include plural forms, unless otherwise clearly indicated herein. The terms "comprise", "include" and "have" are inclusive and therefore specify the presence of features, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, steps, operations, elements, components and / or combinations thereof. The method steps, processes and operations described herein should not be interpreted as necessarily requiring them to be performed in the particular order discussed or shown, unless specifically identified as an execution order. Additional or alternative steps may be adopted.

[0030] The following embodiments of the present invention are described in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features therein may be combined with each other.

[0031] An embodiment of the present disclosure provides a skin care composition, comprising the following components in parts by mass: 4.5-5.5 parts of deionized water, 1-3 parts of glycerin, 1.5-2.5 parts of butylene glycol, 0.005-0.02 parts of caprylyl glycol, 0.0008-0.002 parts of acetyl hexapeptide-8, and 0.2-0.4 parts of hydroxypropyl tetrahydropyrantriol.

[0032] Specifically, muscle contraction occurs at the synapse (also known as the neuromuscular junction (NMJ), involving both nerves and muscles. The SNARE complex: vesicle-associated membrane protein (VAMP), synaptosome-associated protein (SNAP-25), and syntaxin. This complex is responsible for releasing ACh into the synapse. Upon stimulation of neuronal vesicles containing ACh, the SNARE complex assembles and releases the neurotransmitter into the synapse. ACh receptors then bind to ACh, causing muscle contraction.

[0033] Specifically, acetyl hexapeptide-8 is a hexapeptide that competes with SNAP-25 for its position in the SNARE complex, thereby regulating the formation of the complex and interfering with the formation and stability of the complex, but it is very safe. That is, acetyl hexapeptide-8 interferes with the SNARE complex, reduces muscle contraction, regulates the release of glutamate, and reduces wrinkles.

[0034] Specifically, hydroxypropyl tetrahydropyrantriol can directly affect the extracellular matrix in the three-layer structure of the skin, affect the secretion of glycosaminoglycans (GAGs), promote the synthesis of GAGs in the skin, improve the adhesion between the dermis and the epidermis, promote the regeneration of damaged tissues, help maintain the elasticity of the dermis, effectively keep the skin firm and delicate, and delay skin aging.

[0035] The presently disclosed embodiments also provide an anti-wrinkle and firming lotion comprising the following components: Phase A comprising: 80-85 parts of deionized water, 4-8 parts of glycerin, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, and 0.01-0.03 parts of xanthan gum; Phase B comprising: 0.3-0.6 parts of parahydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase C comprising: 6-10 parts of the aforementioned skin care composition.

[0036] The presently disclosed embodiments further provide a method for preparing the aforementioned anti-wrinkle and firming water, comprising the following steps: step S11, pretreatment, i.e., mixing and heating phase A uniformly, and heating and stirring phase B until dispersed until transparent; step S12, cooling phase A and adding it to phase B and stirring uniformly to obtain an AB mixed phase; step S13, cooling the AB mixed phase, adding phase C and stirring and dispersing uniformly to obtain a mixed phase; step S14, cooling the mixed phase to room temperature to obtain the anti-wrinkle and firming water.

[0037] Specifically, in step S11, phase A is heated to 85° C., and phase B is heated to 60° C.; in step S12, phase A is cooled to 60° C.; and in step S13, the AB mixed phase is cooled to 45° C.

[0038] The presently disclosed embodiment also provides an anti-wrinkle and firming lotion, comprising the following components: Phase A, comprising: 70-75 parts of deionized water, 6-10 parts of glycerin, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, 0.01-0.03 parts of xanthan gum, and 0.05-0.2 parts of sodium stearoyl glutamate; Phase B, comprising: 1-2 parts of C14-22 alcohol, 0.3-0.5 parts of C12-20 alkyl glucoside, 0.5-1.5 parts of stearyl alcohol, 1-3 parts of squalane, and 3-5 parts of polydimethylsiloxane; Phase C, comprising: 0.3-0.6 parts of p-hydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase D, comprising: 6-10 parts of the skin care composition described above.

[0039] The present disclosure also provides a method for preparing the aforementioned anti-wrinkle and firming lotion, comprising the following steps: step S21, pretreatment, i.e., mixing and heating phase A uniformly, and heating and stirring phase C to disperse until transparent; step S22, heating phase B and adding it to phase A, and homogenizing to obtain an AB mixed phase; step S23, cooling the AB mixed phase, adding phase D, and homogenizing to obtain an ABD mixed phase; step S24, cooling the ABD mixed phase, adding phase C, and stirring and dispersing uniformly to obtain a mixed phase; step S25, continuing to stir the mixed phase and cooling it to room temperature to obtain the anti-wrinkle and firming lotion.

[0040] Specifically, in step S21, phase A is heated to 85°C and phase C is heated to 60°C; in step S22, phase B is heated to 85°C; in step S23, the AB mixed phase is cooled to 60°C; and in step S24, the ABD mixed phase is cooled to 45°C.

[0041] The present disclosure also provides an anti-wrinkle and firming cream comprising the following components: Phase A, comprising: 65-70 parts of deionized water, 6-10 parts of glycerin, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, 0.01-0.03 parts of xanthan gum, and 0.05-0.2 parts of sodium stearoyl glutamate; Phase B, comprising: 1-3 parts of C14-22 alcohol, 0.4-0.6 parts of C12-20 alkyl glucoside, 3-5 parts of stearyl alcohol, 2-4 parts of squalane, and 3-5 parts of polydimethylsiloxane; Phase C, comprising: 0.3-0.6 parts of p-hydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase D, comprising: 6-10 parts of the skin care composition described above.

[0042] The presently disclosed embodiments further provide a method for preparing the anti-wrinkle and firming cream as described above, comprising the following steps: step S31, pretreatment, i.e., mixing and heating phase A uniformly, and heating and stirring phase C to disperse until transparent; step S32, heating phase B and adding it to phase A, and homogenizing to obtain an AB mixed phase; step S33, cooling the AB mixed phase, adding phase D, and homogenizing to obtain an ABD mixed phase; step S34, cooling the ABD mixed phase, adding phase C, and stirring and dispersing uniformly to obtain a mixed phase; step S35, continuing to stir the mixed phase and cooling it to room temperature to obtain the anti-wrinkle and firming cream.

[0043] Specifically, in step S31, phase A is heated to 85°C and phase C is heated to 60°C; in step S32, phase B is heated to 85°C; in step S33, the AB mixed phase is cooled to 60°C; and in step S34, the ABD mixed phase is cooled to 45°C.

[0044] Example 1

[0045] Formula of skin care composition:

[0046] 4.5 parts of deionized water, 1 part of glycerol, 1.5 parts of butylene glycol, 0.005 parts of caprylyl glycol, 0.0008 parts of acetyl hexapeptide-8, and 0.2 parts of hydroxypropyl tetrahydropyrantriol.

[0047] The application of the skin care composition in skin care water has the following formula:

[0048] Phase A: deionized water 82.93g, glycerol 6g, 1,3-propylene glycol 2g, disodium EDTA 0.05g, xanthan gum 0.02g;

[0049] Phase B: 0.5 g p-hydroxyacetophenone, 0.5 g 1,2-hexanediol;

[0050] Phase C: 8g of anti-wrinkle, firming and moisturizing composition.

[0051] Production steps:

[0052] Step S11: Mix phase A evenly and heat to 85°C, heat phase B to 60°C, stir and disperse until transparent, and set aside;

[0053] Step S12, cooling phase A to 60°C, adding phase B, and stirring evenly to obtain an AB mixed phase;

[0054] Step S13, cooling the AB mixed phase to 45°C, adding the C phase and stirring and dispersing them uniformly to obtain a mixed phase;

[0055] Step S14, cooling the mixed phase to room temperature to obtain a sample of anti-wrinkle and firming water.

[0056] Test Name: Zebrafish Moisturizing Efficacy Test Method

[0057] Method source: "Standard operating procedures for the evaluation of the moisturizing effect of zebrafish"

[0058] The trial starts and ends on August 15, 2023, and ends on August 16, 2023.

[0059] Brief description of results

[0060] 1. System and sample size

[0061] Experimental system: zebrafish (Albino) with melanin allele mutation.

[0062] Zebrafish age: 2 days post fertilization (2 dpf).

[0063] The sample size of each experimental group was 15 (N=10).

[0064] Adult fish rearing and breeding methods: follow the company's laboratory standard rearing and breeding methods, in line with the requirements of the international AAALAC certification (certification number: 001458).

[0065] 2. Principle and method: When zebrafish are treated with sodium chloride, the skin surface will lose water and shrink due to osmotic pressure, and the tail area will become smaller due to shrinkage. The moisturizing effect of cosmetics can be evaluated by the impact on the tail area.

[0066] 3. Experimental Procedure

[0067] S1. Randomly select zebrafish and place them in a 6-well plate, with 15 zebrafish per well.

[0068] S2. Dissolve the sample in water and set up a normal control group and a model control group. The volume of each well is 3 mL.

[0069] S3. Sodium chloride was simultaneously administered in water to establish a zebrafish skin dehydration model.

[0070] S4. Incubate in the dark at 28°C for 22 h.

[0071] Ten zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Advanced image processing software was used to analyze and collect data. The tail area (S) of the zebrafish was analyzed, and the moisturizing effect of the sample was calculated according to the formula to determine whether it has moisturizing effect.

[0072] Moisturizing effect (%) = (S (sample group) - S (model control group)) / (S (normal control group) - S (model control group)) * 100%

[0073] 4. Applicability and Limitations

[0074] Applicable to moisturizing efficacy testing of cosmetics and their raw materials. It requires that the sample can be dissolved in water or prepared into a suspension that can be evenly dispersed in water.

[0075] 5. Basis for determination

[0076] Statistical analysis showed that p<0.05 was significant.

[0077] Applicability and Limitations: This test is applicable to the moisturizing efficacy test of cosmetics and their raw materials. The sample must be soluble in water or be prepared into a suspension that can be evenly dispersed in water.

[0078] The difference was determined to be significant if p<0.05 according to statistical analysis.

[0079] Specifically, the test results are shown in Table 1 below.

[0080] Table 1 Test results

[0081] Test items Detection concentration (%) effect(%) p-value Test results Moisturizing effect 0.025 47 <0.01 significant

[0082] Specifically, the detection concentration of 0.025% refers to diluting the anti-wrinkle and firming water sample in the anti-wrinkle and firming water sample group to a concentration of 0.025%.

[0083] Specifically, such as Figure 1 As shown, the normal control group was fish farming water, the model control group was 0.9% sodium chloride + fish farming water, and the anti-wrinkle and firming water group was 0.9% sodium chloride + fish farming water + 0.025% sample anti-wrinkle and firming water; Figure 1 The red dotted line is the body part of the zebrafish, and the shaded area is the tail of the zebrafish. The tail area of the anti-wrinkle and firming water group is significantly increased compared with the model control group, revealing that the sample has moisturizing effect.

[0084] Example 2

[0085] Formula of skin care composition:

[0086] 5 parts of deionized water, 2 parts of glycerol, 2 parts of butylene glycol, 0.01 parts of caprylyl glycol, 0.001 parts of acetyl hexapeptide-8, and 0.3 parts of hydroxypropyl tetrahydropyrantriol.

[0087] The application of the skin care composition in the emulsion has the following formula:

[0088] Phase A: deionized water 71.65g, glycerol 8g, 1,3-propylene glycol 2g, disodium EDTA 0.05g, xanthan gum 0.2g, sodium stearoyl glutamate 0.1g;

[0089] Phase B: C14-22 alcohol 1.6g, C12-20 alkyl glucoside 0.4g, stearyl alcohol 1g, squalane 2g, dimethicone 4g;

[0090] Phase C: 0.5 g p-hydroxyacetophenone, 0.5 g 1,2-hexanediol;

[0091] Phase D: 8g of anti-wrinkle, firming and moisturizing composition.

[0092] Production steps:

[0093] Step S21, mix phase A evenly and heat to 85°C, heat phase C to 60°C, stir and disperse until transparent, and set aside;

[0094] Step S22, heating phase B to 85°C, adding phase B to phase A, and homogenizing for 5 minutes to obtain an AB mixed phase;

[0095] Step S23, cooling the AB mixed phase to 60°C, adding phase D, and homogenizing for 5 minutes to obtain an ABD mixed phase;

[0096] Step S24, stirring the ABD mixed phase and cooling it to 45°C, adding the pretreated C phase, stirring and dispersing it evenly to obtain a mixed phase.

[0097] Step S25, continue stirring and cool down to room temperature to obtain a sample anti-wrinkle firming lotion.

[0098] Test Name: Zebrafish Firming Efficacy Test Method

[0099] Method source: "T / ZHCA015-2022 Evaluation of the Firming Efficacy of Cosmetics: Relative Expression of Elastin Gene in Zebrafish Juveniles"

[0100] The trial starts and ends on August 14, 2023, and ends on August 16, 2023.

[0101] Brief description of results

[0102] 1. System and sample size

[0103] Experimental system: Wild-type AB strain zebrafish.

[0104] Zebrafish age: 4 days post fertilization (4 dpf).

[0105] Sample size for each experiment: 30 (three biological replicates, N=3)

[0106] Adult fish rearing and breeding methods: follow the company's laboratory standard rearing and breeding methods, in line with the requirements of the international AAALAC certification (certification number: 001458).

[0107] 2. Principle and method

[0108] Elastin is the primary component of elastic fibers in skin tissue, providing structural support and protecting the skin from aging and sagging. Elastin is composed of two alternating short peptides. Eln1 and eln2 encode different elastin peptides. Together, these two genes regulate elastin expression, contributing to skin firmness and elasticity. Zebrafish possess elastin genes (eln1 and eln2) similar to those found in humans. Therefore, measuring the relative expression of the ehn1 and / or eln2 genes can indicate whether a sample has firming properties.

[0109] 3. Experimental Procedure

[0110] S1. Randomly select zebrafish and place them in a 6-well plate, with 30 zebrafish per well.

[0111] S2. Water-soluble administration samples and a normal control group were set up simultaneously. The volume of each well was 3 mL. Three biological replicates were performed.

[0112] S3. Incubate in the dark at 28°C for 24 hours.

[0113] S4. Total RNA was extracted from zebrafish in each experimental group, cDNA was synthesized, and the gene expression of β-actm and target genes was detected by q-PCR.

[0114] S5. Use β-actin as an internal reference for gene expression to calculate the relative RNA expression of the target gene.

[0115] Relative RNA expression = 2 ΔΔC(t)

[0116] ΔΔC(t)=ΔC(t) 正常对照组 -ΔC(t) 样品组

[0117] ΔC(t)=C(t) 目的基因 -C(t) β-actin

[0118] 4. Applicability and Limitations

[0119] Applicable to the firming efficacy test of cosmetics and their raw materials. It requires that the sample can be dissolved in water or prepared into a suspension that can be evenly dispersed in water.

[0120] 5. Basis for determination

[0121] Statistical analysis showed that p<0.05 was significant.

[0122] Specifically, the test results are shown in Table 2 below.

[0123] Table 2 Test results

[0124]

[0125] Specifically, the detection concentration of 0.05% refers to diluting the sample anti-wrinkle and firming lotion in the sample anti-wrinkle and firming lotion group to a concentration of 0.05%.

[0126] Specifically, such as Figure 2 As shown, the relative expression level of eln1 gene in the anti-wrinkle firming lotion group was significantly increased compared with the normal control group, revealing that the sample has a firming effect.

[0127] Example 3

[0128] Formula of skin care composition:

[0129] 5.5 parts of deionized water, 3 parts of glycerol, 2.5 parts of butylene glycol, 0.02 parts of caprylyl glycol, 0.002 parts of acetyl hexapeptide-8, and 0.4 parts of hydroxypropyl tetrahydropyrantriol.

[0130] The application of the skin care composition in anti-wrinkle and firming cream has the following formula:

[0131] Phase A: deionized water 67.65g, glycerol 8g, 1,3-propylene glycol 2g, disodium EDTA 0.05g, xanthan gum 0.2g, sodium stearoyl glutamate 0.1g;

[0132] Phase B: C14-22 alcohol 2g, C12-20 alkyl glucoside 0.5g, stearyl alcohol 3.5g, squalane 3g, dimethicone 4g;

[0133] Phase C: 0.5 g p-hydroxyacetophenone, 0.5 g 1,2-hexanediol;

[0134] Phase D: 8g of anti-wrinkle, firming and moisturizing composition.

[0135] Production steps:

[0136] Step S31, mix phase A evenly and heat to 85°C, heat phase C to 60°C, stir and disperse until transparent, and set aside.

[0137] Step S32, heating phase B to 85°C, adding phase B to phase A, and homogenizing for 5 minutes to obtain an AB mixed phase;

[0138] Step S33, cooling the AB mixed phase to 60°C, adding phase D, and homogenizing for 5 minutes to obtain an ABD mixed phase;

[0139] Step S34, stirring the ABD mixed phase and cooling it to 45°C, adding the pretreated C phase, stirring and dispersing it evenly to obtain a mixed phase.

[0140] Step S35, continue stirring and cool down to room temperature to obtain a sample anti-wrinkle firming cream.

[0141] Test Name: Zebrafish Anti-Wrinkle Efficacy Test Method

[0142] Method source: "Standard operating procedures for zebrafish anti-wrinkle efficacy evaluation experiment"

[0143] The trial starts and ends on August 14, 2023, and ends on August 16, 2023.

[0144] Brief description of results

[0145] 1. System and sample size

[0146] Experimental system: Wild-type AB strain zebrafish.

[0147] Zebrafish age: 4 days post fertilization (4 dpf).

[0148] Sample size for each experiment: 30 (three biological replicates, N=3)

[0149] Adult fish rearing and breeding methods: follow the company's laboratory standard rearing and breeding methods, in line with the requirements of the international AAALAC certification (certification number: 001458).

[0150] 2. Principle and method

[0151] Skin growth, repair, nutrition, elasticity, and tension are all related to collagen. Its loss can reduce skin smoothness and produce wrinkles. In tetrapods, type I collagen is a trimer mainly composed of two αl chains and one α2 chain, encoded by the collala and colla2 genes, respectively, and performs collagen-related biological functions in connective tissue and bone. In zebrafish, there are three type I collagen genes, collala, collalb, and colla2, which encode the αl(I), α2(I), and α3(I) chains, respectively. Therefore, by detecting the relative expression levels of the collala or (and) collalb or (and) colla2 genes, it can be indicated whether the sample has anti-wrinkle effects.

[0152] Experimental procedures

[0153] S1. Randomly select zebrafish and place them in a 6-well plate, with 30 zebrafish per well.

[0154] S2. Water-soluble administration samples and a normal control group were set up simultaneously. The volume of each well was 3 mL. Three biological replicates were performed.

[0155] S3. Incubate in the dark at 28°C for 24 hours.

[0156] S4. Total RNA of zebrafish in each experimental group was extracted, cDNA was synthesized, and the gene expression of β-actin and target genes was detected by q-PCR.

[0157] S5. Use β-actin as an internal reference for gene expression to calculate the relative RNA expression of the target gene.

[0158] Relative RNA expression = 2 ΔΔC(t)

[0159] ΔΔC(t)=ΔC(t) 正常对照组 -ΔC(t) 样品组

[0160] ΔC(t)=C(t) 目的基因 -C(t) β-actin

[0161] 4. Applicability and Limitations

[0162] Applicability and Limitations: Applicable to the anti-wrinkle efficacy testing of cosmetics and their raw materials. The sample must be soluble in water or prepared into a suspension that can be evenly dispersed in water.

[0163] 5. Basis for determination

[0164] Statistical analysis showed that p<0.05 was significant.

[0165] Specifically, the test results are shown in Table 3 below.

[0166] Table 3 Test results

[0167]

[0168] Specifically, the detection concentration of 0.05% refers to diluting the sample anti-wrinkle and firming cream in the sample anti-wrinkle and firming cream group to a concentration of 0.05%.

[0169] Specifically, such as Figure 3 As shown, the relative expression level of col1a1a gene in the anti-wrinkle firming cream group was significantly increased compared with the normal control group, revealing that the sample has anti-wrinkle effect.

[0170] In summary, the present composition, cosmetics and preparation method with anti-wrinkle and firming effects utilize acetyl hexapeptide-8 to compete with synaptosome-associated protein SNAP-25 for a position in the SNARE complex by compounding the composition, thereby interfering with the formation of the complex and reducing muscle contraction to reduce wrinkles. At the same time, hydroxypropyl tetrahydropyrantriol in the composition system can affect the extracellular matrix in the three-layer structure of the skin, effectively keeping the skin firm and delicate, thereby delaying skin aging, so that the skin care product has both anti-wrinkle and firming effects, and also avoids the disadvantages of some active ingredients being unstable and irritating to the skin.

[0171] With the above-described preferred embodiments of the present invention as a guide, and with reference to the above description, relevant personnel are fully capable of making various changes and modifications without departing from the technical scope of this invention. The technical scope of this invention is not limited to the contents of the specification and must be determined according to the scope of the claims.

Claims

1. A skin care composition, characterized in that The composition includes the following parts by mass: 4.5-5.5 parts of deionized water, 1-3 parts of glycerol, 1.5-2.5 parts of butylene glycol, 0.005-0.02 parts of caprylyl glycol, 0.0008-0.002 parts of acetyl hexapeptide-8, and 0.2-0.4 parts of hydroxypropyl tetrahydropyrantriol.

2. An anti-wrinkle and firming lotion, characterized in that: Includes the following components: Phase A comprises: 80-85 parts of deionized water, 4-8 parts of glycerol, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, and 0.01-0.03 parts of xanthan gum; Phase B, comprising: 0.3-0.6 parts of p-hydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase C comprises: 6-10 parts of the skin care composition according to claim 1.

3. A method for preparing the anti-wrinkle and firming lotion according to claim 2, characterized in that: The steps include: Step S11, pretreatment, that is, mixing and heating phase A uniformly, heating and stirring phase B until it is transparent, and the temperature of phase A is higher than that of phase B; Step S12, cooling phase A and adding it to phase B and stirring evenly to obtain an AB mixed phase; Step S13, cooling the AB mixed phase, adding the C phase and stirring and dispersing them uniformly to obtain a mixed phase; Step S14, cooling the mixed phase to room temperature to obtain anti-wrinkle and firming water.

4. The preparation method according to claim 3, wherein In step S11, phase A is heated to 85°C and phase B is heated to 60°C; In step S12, the temperature of phase A is reduced to 60° C. In step S13, the AB mixed phase is cooled to 45°C.

5. An anti-wrinkle firming lotion, characterized in that: Includes the following components: Phase A comprises: 70-75 parts of deionized water, 6-10 parts of glycerol, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, 0.01-0.03 parts of xanthan gum, and 0.05-0.2 parts of sodium stearoyl glutamate; Phase B, including: 1-2 parts of C14-22 alcohol, 0.3-0.5 parts of C12-20 alkyl glucoside, 0.5-1.5 parts of stearyl alcohol, 1-3 parts of squalane, and 3-5 parts of polydimethylsiloxane; Phase C, comprising: 0.3-0.6 parts of p-hydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase D comprises: 6-10 parts of the skin care composition according to claim 1.

6. A method for preparing the anti-wrinkle and firming lotion according to claim 5, characterized in that: The steps include: Step S21, pretreatment, that is, mixing and heating phase A uniformly, and heating and stirring phase C to disperse until transparent; Step S22, heating phase B and adding it to phase A, and homogenizing to obtain an AB mixed phase; Step S23, cooling the AB mixed phase, adding the D phase, and homogenizing to obtain an ABD mixed phase; Step S24, cooling the ABD mixed phase, adding phase C, stirring and dispersing the mixture uniformly to obtain a mixed phase; Step S25, continuing to stir the mixed phase and cooling it to room temperature to obtain an anti-wrinkle and firming lotion.

7. The preparation method according to claim 6, wherein In step S21, phase A is heated to 85°C and phase C is heated to 60°C; In step S22, phase B is heated to 85° C. In step S23, the AB mixed phase is cooled to 60°C; In step S24, the ABD mixed phase is cooled to 45°C.

8. An anti-wrinkle and firming cream, characterized in that: Includes the following components: Phase A comprises: 65-70 parts of deionized water, 6-10 parts of glycerin, 1-3 parts of 1,3-propylene glycol, 0.03-0.06 parts of disodium EDTA, 0.01-0.03 parts of xanthan gum, and 0.05-0.2 parts of sodium stearoyl glutamate; Phase B, including: 1-3 parts of C14-22 alcohol, 0.4-0.6 parts of C12-20 alkyl glucoside, 3-5 parts of stearyl alcohol, 2-4 parts of squalane, and 3-5 parts of polydimethylsiloxane; Phase C, comprising: 0.3-0.6 parts of p-hydroxyacetophenone and 0.3-0.6 parts of 1,2-hexanediol; Phase D comprises: 6-10 parts of the skin care composition according to claim 1.

9. A method for preparing the anti-wrinkle and firming cream according to claim 8, characterized in that: The steps include: Step S31, pretreatment, that is, mixing and heating phase A uniformly, and heating and stirring phase C to disperse until transparent; Step S32, heating phase B and adding it to phase A, and homogenizing to obtain an AB mixed phase; Step S33, cooling the AB mixed phase, adding the D phase, and homogenizing to obtain an ABD mixed phase; Step S34, cooling the ABD mixed phase, adding phase C, stirring and dispersing the mixture uniformly to obtain a mixed phase; Step S35, continuing to stir the mixed phase and cooling it to room temperature to obtain an anti-wrinkle and firming cream.

10. The preparation method according to claim 9, characterized in that In step S31, phase A is heated to 85°C and phase C is heated to 60°C; In step S32, phase B is heated to 85° C. In step S33, the AB mixed phase is cooled to 60°C; In step S34, the temperature of the ABD mixed phase is reduced to 45°C.

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