Tightening and anti-wrinkle composition and application thereof

Abstract

The invention discloses a firming and anti-wrinkle composition and application thereof, and belongs to the field of cosmetics. The composition comprises the following raw materials: a tuber melanosporum extract, a dendrobium officinale extract, a polyrock extract and a swertia indica extract, and the raw materials are synergistically compounded, so that the composition provided by the invention has remarkable tightening and anti-wrinkle effects.

Description

Technical Field

[0001] The present invention belongs to the field of cosmetics, and in particular relates to a firming and anti-wrinkle composition and application thereof. Background Art

[0002] Current anti-wrinkle skin care products mainly rely on the following technical routes:

[0003] Synthetic ingredients: Improve wrinkles by stimulating collagen regeneration, but have problems such as high irritation (the "Cosmetic Safety Technical Specifications" limit the use of retinol to ≤1%) and poor photostability;

[0004] Single plant extracts: They are safer than synthetic ingredients, but they have a single target (only antioxidant or anti-inflammatory), making it difficult to address the complex mechanisms of photoaging, including oxidative stress, inflammatory response, and matrix degradation.

[0005] Traditional compound combinations: Mostly based on empirical combinations, lacking the synergistic design of key targets such as elastase inhibition, DNA repair, and signal pathway regulation, resulting in limited efficacy improvement.

[0006] In view of the above problems, there is an urgent need to provide a multi-target synergistic firming and anti-wrinkle composition to solve the defects of poor stability, single efficacy and high irritation in the existing technology. Summary of the Invention

[0007] The present invention provides a firming and anti-wrinkle composition and its application. The composition has the effect of firming and anti-wrinkle by synergistically compounding a Tuber melanosporum extract, a Dendrobium officinale extract, a Cistus polyphylla extract and a Swertia indica extract.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a firming and anti-wrinkle composition, comprising the following raw materials in parts by weight:

[0010] Black Truffle Extract: 3-9 parts;

[0011] Dendrobium officinale extract: 1-5 parts;

[0012] Cistus polyphylla extract: 0.2-0.8 parts;

[0013] Indian swede sprout extract: 0.1-0.5 parts.

[0014] Preferably, the composition comprises the following raw materials in parts by weight:

[0015] Black Truffle Extract: 6-9 parts;

[0016] Dendrobium officinale extract: 3-5 parts;

[0017] Cistus polyphylla extract: 0.4-0.8 parts;

[0018] Indian swede sprout extract: 0.3-0.5 parts.

[0019] Preferably, the composition comprises the following raw materials in parts by weight:

[0020] Black Truffle Extract: 7 parts;

[0021] Dendrobium officinale extract: 4 parts;

[0022] Cistus polyphylla extract: 0.5 parts;

[0023] Indian Swertia Sprout Extract: 0.4 parts.

[0024] In a second aspect, the present invention provides a use of the firming and anti-wrinkle composition of the first aspect in preparing a skin care product with firming and anti-wrinkle effects.

[0025] Preferably, the dosage form of the skin care product is any one of lotion, cream, essence, eye cream, lotion, and facial mask.

[0026] In a third aspect, the present invention provides an essence with firming and anti-wrinkle effects, the essence comprising the following components in percentage by weight:

[0027] The composition of the first aspect: 1-10 wt%;

[0028] Moisturizing agent: 0.01-5wt%;

[0029] Emollient: 0.01-2wt%;

[0030] Thickener: 0.1-1wt%;

[0031] Preservative: 0.01-0.5wt%;

[0032] Deionized water was added to make up to 100 wt%.

[0033] Preferably, the moisturizing agent is at least one of sodium hyaluronate, glycerin, and butylene glycol.

[0034] Preferably, the emollient is at least one of squalane, dimethicone, and caprylic / capric triglyceride.

[0035] Preferably, the thickener is at least one of sodium polyacryloyldimethyltaurate and ammonium acryloyldimethyltaurate / VP copolymer.

[0036] Preferably, the preservative is at least one of phenoxyethanol and ethylhexylglycerin.

[0037] In the present invention:

[0038] Tuber melanosporum extract: Tuber melanosporum, a fungus of the genus Tuber in the family Tuberaceae, is commonly known as "truffle" and is primarily found in Europe and southwestern China. Its extract is rich in truffle polysaccharides, triterpenoids, and essential amino acids. It activates macrophages and promotes collagen synthesis, strengthening the skin barrier function and reducing wrinkles and sagging.

[0039] Dendrobium Officinale Extract: Dendrobium Officinale, a plant of the genus Dendrobium in the Orchidaceae family, is rich in polysaccharides, dendrobine, amino acids, vitamins, flavonoids, etc. It has multiple functions such as antioxidant, anti-aging, and skin care.

[0040] Cistus monspeliensis Extract: Cistus monspeliensis, a plant of the genus Cistus in the family Cistusaceae, is primarily found in the barren mountainous regions along the Mediterranean coast. The extract's core active ingredients are myricetin-3-O-rhamnoside and Ciste'M. These active ingredients promote the synthesis of DNA repair enzymes (such as XPC and DDB2), reduce UVA / UVB and blue light-induced DNA damage and lipid peroxidation, inhibit collagen degradation by the metalloproteinase MMP-1, stimulate dermal collagen regeneration, accelerate epidermal renewal, and strengthen basement membrane structure, improving wrinkles and skin sagging.

[0041] Swertia chirayata extract: Swertia chirayata (Swertia chirayita), a member of the Gentianaceae family, is primarily found in the Himalayas. Its main active ingredients are swertiamarin and iridoid compounds. These active substances can inhibit the expression of inflammatory factors (such as ICAM-1), reduce UV-induced erythema and skin sensitivity, scavenge free radicals, reduce oxidative stress-induced skin damage, and delay photoaging.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] This invention combines extracts from Tuber melanosporum, Dendrobium officinale, Cistus polyphylla, and Swertia indica. These ingredients form a three-dimensional defense network through antioxidant, anti-inflammatory, collagen protection, and cell regeneration benefits. This combination directly inhibits elastase activity and indirectly reduces elastase expression by repairing the skin's microenvironment. This synergistic effect can significantly improve firmness and elasticity in anti-aging skincare products, making them suitable for repairing photoaging and dynamic wrinkles. DETAILED DESCRIPTION

[0044] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples. The terms used in the examples are for describing specific embodiments and do not constitute a limitation on the scope of protection of the present invention.

[0045] The experimental methods in the following examples, where specific conditions are not specified, are generally based on conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0046] Some of the raw materials and their sources are as follows:

[0047] Black Truffle Extract: Shanghai Haotai Biotechnology Co., Ltd.

[0048] Dendrobium officinale extract: Yunnan Yingge Biotechnology Co., Ltd.

[0049] Cistus oleifera extract: BASF (China) Co., Ltd.

[0050] Indian Swertia Sprout Extract: LUCAS MEYER COSMETICS

[0051] Other raw materials and reagents were purchased commercially.

[0052] A firming and anti-wrinkle composition, the raw materials and their weight parts are as follows:

[0053] Composition 1:

[0054] Black Truffle Extract: 7 parts;

[0055] Dendrobium officinale extract: 4 parts;

[0056] Cistus polyphylla extract: 0.5 parts;

[0057] Indian Swertia Sprout Extract: 0.4 parts.

[0058] Composition 2:

[0059] Black Truffle Extract: 9 parts;

[0060] Dendrobium officinale extract: 1 part;

[0061] Cistus polyphylla extract: 0.4 parts;

[0062] Indian swede sprout extract: 0.3 parts.

[0063] Composition 3:

[0064] Black Truffle Extract: 3 parts;

[0065] Dendrobium officinale extract: 5 parts;

[0066] Cistus polyphylla extract: 0.2 parts;

[0067] Indian Swertia Sprout Extract: 0.5 parts.

[0068] Composition 4:

[0069] Black Truffle Extract: 6 parts;

[0070] Dendrobium officinale extract: 3 parts;

[0071] Cistus polyphylla extract: 0.8 parts;

[0072] Indian swede sprout extract: 0.1 parts.

[0073] Composition ①: Different from composition 1, it lacks the Tuber Melosporus extract, and the missing mass is supplemented by Dendrobium officinale extract, Cistus polyphylla extract, and Swertia indica extract in a mass ratio of 4:0.5:0.4.

[0074] Composition ②: Different from composition 1, it lacks the Dendrobium officinale extract, and the missing mass is supplemented by the Black Tuber extract, the Cistus polymorpha extract, and the Swertia indica extract in a mass ratio of 7:0.5:0.4.

[0075] Composition ③: Different from composition 1, it lacks the Cistus polymorpha extract, and the missing mass is supplemented by Tuber melanosporus extract, Dendrobium officinale extract, and Swertia indica extract in a mass ratio of 7:4:0.4.

[0076] Composition ④: Different from composition 1, it lacks the Swertia indica extract, and the missing mass is supplemented by Tuber Melospora extract, Dendrobium officinale extract, and Cistus polyphylla extract in a mass ratio of 7:4:0.5.

[0077] Composition ⑤:

[0078] Black Truffle Extract: 0.4 parts;

[0079] Dendrobium officinale extract: 0.5 parts;

[0080] Cistus polyphylla extract: 7 parts;

[0081] Indian Swertia Sprout Extract: 4 parts.

[0082] Elastase inhibition test:

[0083] This test uses porcine pancreatic elastase as the test subject and N-succinyl-alanine-alanine-p-nitroanilide (AAAPVN) as the substrate. Porcine pancreatic elastase hydrolyzes AAAPVN, and the hydrolysis product causes an increase in absorbance at 420 nm. This absorbance is measured using a microplate reader to evaluate the firming and anti-wrinkle efficacy of the composition.

[0084] Solution preparation:

[0085] Preparation of the sample solution to be tested: Use pure water to prepare compositions 1-4 and compositions ①-⑤ into a 5 wt % mixed solution.

[0086] Prepare Tris-HCl buffer: weigh 2.42 g of Tris into a beaker, add 200 mL of ultrapure water, and after complete dissolution, adjust the pH to 8.0 with concentrated HCl to prepare Tris-HCl buffer.

[0087] Prepare a positive control tea polyphenol solution: weigh 5 mg of tea polyphenols and dissolve it in 5 mL of Tris-HCl buffer to prepare a positive control solution.

[0088] Preparation of substrate solution AAAPVN: Weigh 4.51 mg of AAAPCN and dissolve it in 5 mL of Tris-HCl buffer to prepare a substrate solution.

[0089] Preparation of porcine pancreatic elastase solution: porcine pancreatic elastase was measured and dissolved in 10 mL of Tris-HCl buffer to prepare a porcine pancreatic elastase solution with an enzyme activity of 0.171 U / mL.

[0090] Experimental group division:

[0091] The experimental group was divided into four groups: the test sample group, the positive control group, the blank control group, and the model control group. Four replicate wells were set up for each group at the same concentration. The amount of each solution added is shown in Table 1.

[0092] Table 1

[0093]

[0094]

[0095] Determination method: Incubate at room temperature for 15 minutes and measure the absorbance at 420 nm using a microplate reader.

[0096] Calculation formula: Inhibition rate (%) = [1-(A1-A0) / (A2-A0)] × 100%

[0097] Where: A0 is the average absorbance of the blank control group; A1 is the average absorbance of the test sample group or the positive control group; A2 is the average absorbance of the model control group. The experimental results are expressed as average values.

[0098] Table 2

[0099] Sample Group Elastase inhibition rate / % Blank control group / Model control group 0 Positive control group 70.31* Composition 1 68.42* Composition 2 62.73* Composition 3 65.14* Composition 4 67.26* Composition ① 48.37* Composition ② 45.26* Composition ③ 47.35* Composition ④ 40.41* Composition ⑤ 59.53*

[0100] Note: “*” indicates that the positive control group, compositions 1-4, compositions ①-⑤ were compared with the model control group, p<0.05.

[0101] Test results: By comparing the results of composition 1 with compositions ①-⑤, it can be seen that the extract of Tuber melanosporum, the extract of Dendrobium officinale, the extract of Cistus polyphylla and the extract of Swertia indica in the present invention have a significant synergistic effect in inhibiting the activity of elastase.

[0102] Specifically, by comparing the results of composition 1 with compositions ①-④, it can be seen that there is a certain synergistic effect between the black truffle extract, the Dendrobium officinale extract, the Cistus polysaccharide extract, and the Indian sedge sprout extract. The possible mechanism is: the truffle polysaccharides and triterpenoid compounds in the black truffle extract prolong cell life by activating telomerase (SIRT-1 pathway) and enhance the collagen regeneration ability of fibroblasts. At the same time, the ceramides and essential amino acids it contains can repair the skin barrier and reduce the release of elastase caused by external stimuli; the polysaccharides and phenanthrene compounds in the Dendrobium inhibit oxidative stress by scavenging free radicals and reduce the oxidative microenvironment required for elastase activation; the active substances such as myricetin glycoside in the Cistus polysaccharide can inhibit the activity of MMP-1 and MMP-3, reduce the degradation of collagen and elastase, and promote the synthesis of DNA repair enzymes such as XPC and DDB2, repair damaged skin cells, and thus reduce the source of elastase release; the sedge sprout bitter glycoside in the Indian sedge sprout can By inhibiting the NF-kb and JAK2 / STAT3 signaling pathways, reducing the release of proinflammatory factors (such as TNF-α, IL-6), and blocking the excessive activation of elastase induced by inflammation, the iridoid compounds it contains can downregulate the activity of TRPV1 receptors and alleviate the damage of neurogenic inflammation to the dermis. The above four raw materials are used in combination, among which Dendrobium officinale extract and Black Truffle extract can remove free radicals and reduce oxidative stress, Indian musk deer sprout extract can inhibit verification signals, and the three together can destroy the activation environment of elastase; Polygonum multiflorum extract can inhibit the degradation of matrix metalloproteinases (MMPs), Dendrobium officinale extract promotes collagen synthesis, and Black Truffle extract can prolong cell life. The three together can form a closed loop from inhibiting decomposition to accelerating regeneration; Indian musk deer sprout extract, Dendrobium officinale extract, and Polygonum multiflorum extract block the upstream and downstream activation links of elastase from different pathways, further improving the elastase inhibition rate. Comparison of composition 1 and composition ⑤ shows that when the dosage ratio of each raw material in the composition is within the range defined by the present invention, the composition has a better effect of inhibiting the activity of elastase.

[0103] A firming and anti-wrinkle essence, the components and their mass percentages are as follows

[0104] Essence 1:

[0105] Firming and anti-wrinkle composition (Composition 1): 5 wt%;

[0106] Sodium hyaluronate: 2wt%;

[0107] Squalane: 1.3wt%;

[0108] Sodium polyacryloyldimethyl taurate: 0.3wt%;

[0109] Phenoxyethanol: 0.3wt%;

[0110] Deionized water: make up to 100 wt%.

[0111] Essence 2:

[0112] The difference from essence 1 is that composition 2 is used to replace composition 1, and the remaining components and mass percentages are the same as those of essence 1.

[0113] Essence 3:

[0114] The difference from essence 1 is that composition 3 is used to replace composition 1, and the remaining components and mass percentages are the same as those of essence 1.

[0115] Essence 4:

[0116] The difference from essence 1 is that composition 4 is used to replace composition 1, and the remaining components and mass percentages are the same as those of essence 1.

[0117] Composition 5:

[0118] Firming and anti-wrinkle composition (Composition 1): 1 wt%;

[0119] Sodium hyaluronate: 0.01wt%;

[0120] Squalane: 2wt%;

[0121] Sodium polyacryloyldimethyl taurate: 1wt%;

[0122] Phenoxyethanol: 0.5wt%;

[0123] Deionized water: make up to 100 wt%.

[0124] Essence 6:

[0125] Firming and anti-wrinkle composition (Composition 1): 10 wt%;

[0126] Sodium hyaluronate: 5wt%;

[0127] Squalane: 0.01wt%;

[0128] Sodium polyacryloyldimethyl taurate: 0.1wt%;

[0129] Phenoxyethanol: 0.01wt%;

[0130] Deionized water: make up to 100 wt%.

[0131] Blank Essence:

[0132] Sodium hyaluronate: 2wt%;

[0133] Squalane: 1.3wt%;

[0134] Sodium polyacryloyldimethyl taurate: 0.3wt%;

[0135] Phenoxyethanol: 0.3wt%;

[0136] Deionized water: make up to 100 wt%.

[0137] The specific preparation steps of the above-mentioned essences 1-6 with firming and anti-wrinkle effects are as follows:

[0138] S1: mixing deionized water and the firming and anti-wrinkle composition and stirring to obtain a mixed solution;

[0139] S2: Sodium hyaluronate, squalane, sodium polyacryloyldimethyl taurate, and phenoxyethanol are sequentially added to the mixed solution, and the mixture is stirred to obtain the essence.

[0140] The specific preparation steps of the blank essence are as follows:

[0141] Sodium hyaluronate, squalane, sodium polyacryloyldimethyl taurate, and phenoxyethanol were sequentially added to deionized water, and the mixture was stirred to obtain the blank essence.

[0142] Safety trials

[0143] Using the 2015 "Technical Specifications for Safety of Cosmetics" as a reference standard, the cosmetic irritation evaluation of Essences 1-6 and blank Essence was conducted using a skin patch test on 70 randomly distributed subjects aged 16-65 years, with 10 subjects in each group.

[0144] Test Method: Place the test substance in a patch tester at a dosage of 0.020-0.025g. Cover the patch tester with non-irritating cloth-based tape on the subject's back or forearm. Use the palm of your hand to gently press the patch tester to evenly adhere to the skin surface. Continue for 24 hours. Remove the patch tester and observe the skin reaction 30 minutes after the indentation disappears. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0145] Evaluation criteria:

[0146] Grade 0: negative reaction;

[0147] Grade 1: Suspicious reaction, only slight erythema;

[0148] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0149] Grade 3: Strong positive reaction, with erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0150] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent blister, and reaction beyond the test area.

[0151] Test results: All subjects had negative skin reactions.

[0152] The above test results show that the essence provided by the present invention is mild and non-irritating to the skin and is safe to use.

[0153] Human efficacy testing

[0154] Test samples: Essence 1-6, blank essence.

[0155] Volunteer screening: 210 healthy female subjects (aged 35-55 years) with facial problems such as dry lines, fine lines, sagging, and decreased skin elasticity were selected and randomly divided into 7 groups of 30 people each, with the average age of each group kept as close as possible.

[0156] Test Method: After each group of volunteers cleansed their skin with warm water, a skin elasticity tester was used to measure the skin elasticity parameter R1 on the cheek area. Three measurements were taken and the average was calculated. After the measurement was completed, 3 mL of essence was evenly applied to the face and gently massaged with the fingertips until completely absorbed. This was done once in the morning and evening for 8 consecutive weeks. No other similar products were used during the test period. The test samples were essences 1-6 and a blank essence. All volunteers in the same group used the same products. After the 8-week test, the skin elasticity parameter R2 was measured again on the same cheek area using a skin elasticity tester. Three measurements were taken and the average was calculated. R1 and R2 represent the ratio of the skin's rebound in the absence of negative pressure to its maximum stretch in the presence of negative pressure. They indicate the degree to which the skin can recover within a single cycle after deformation caused by negative pressure. A test value closer to 1 indicates better skin elasticity. See Table 4 for details.

[0157] Skin elasticity improvement rate = (R2-R1) / R1×100%

[0158] Table 4 Skin elasticity test results

[0159] Sample Group Skin elasticity improvement rate / % Essence 1 36.37 Essence 2 33.52 Essence 3 31.69 Essence 4 34.54 Essence 5 17.62 Essence 6 57.62 Blank Essence 1.54

[0160] As shown in Table 4, compared with the blank essence, the skin elasticity improvement rate of essences 1-6 was significantly higher than that of the blank essence, indicating that the composition and essence provided by the present invention have the effect of significantly improving skin elasticity, further proving that the plant extracts in the products of the present invention can effectively maintain and enhance skin elasticity, ultimately achieving a synergistic anti-aging effect.

[0161] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A firming and anti-wrinkle composition, characterized in that: The composition comprises the following raw materials in parts by weight: Black Truffle Extract: 3-9 parts; Dendrobium officinale extract: 1-5 parts; Cistus polyphylla extract: 0.2-0.8 parts; Indian swede sprout extract: 0.1-0.5 parts.

2. The firming and anti-wrinkle composition according to claim 1, characterized in that: The composition comprises the following raw materials in parts by weight: Black Truffle Extract: 6-9 parts; Dendrobium officinale extract: 3-5 parts; Cistus polyphylla extract: 0.4-0.8 parts; Indian swede sprout extract: 0.3-0.5 parts.

3. The firming and anti-wrinkle composition according to claim 1, characterized in that: The composition comprises the following raw materials in parts by weight: Black Truffle Extract: 7 parts; Dendrobium officinale extract: 4 parts; Cistus polyphylla extract: 0.5 parts; Indian Swertia Sprout Extract: 0.4 parts.

4. Use of the firming and anti-wrinkle composition according to any one of claims 1 to 3 in the preparation of a skin care product with firming and anti-wrinkle effects.

5. The application according to claim 4, characterized in that: The dosage form of the skin care product is any one of lotion, cream, essence, eye cream, lotion and mask.

6. A serum with firming and anti-wrinkle effects, characterized in that: Includes the following components by mass percentage: The firming and anti-wrinkle composition according to any one of claims 1 to 3: 1-10 wt%; Moisturizing agent: 0.01-5wt%; Emollient: 0.01-2wt%; Thickener: 0.1-1wt%; Preservative: 0.01-0.5wt%; Deionized water was added to make up to 100 wt%.

7. The essence according to claim 6, characterized in that The moisturizing agent is at least one of sodium hyaluronate, glycerin and butylene glycol.

8. The essence according to claim 6, characterized in that The emollient is at least one of squalane, polydimethylsiloxane, and caprylic / capric triglyceride.

9. The essence according to claim 6, characterized in that The thickener is at least one of sodium polyacryloyldimethyltaurate and ammonium acryloyldimethyltaurate / VP copolymer.

10. The essence according to claim 6, characterized in that The preservative is at least one of phenoxyethanol and ethylhexylglycerin.

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