Cell culture device for stem cell detection of gastrointestinal tumor

Abstract

The invention discloses a cell culture device for stem cell detection of gastrointestinal tumor, and particularly relates to the technical field of cell culture.The cell culture device mainly comprises a rectangular frame column and a linkage screw, the linkage screw is located in the rectangular frame column and rotationally connected with the rectangular frame column, and the top end of the rectangular frame column is fixedly connected with a gear motor used for driving the linkage screw to rotate; a layered linkage mechanism is arranged on the outer wall of the linkage screw rod; the layered linkage mechanism comprises a plurality of sleeving sliding blocks arranged on the outer wall of the linkage screw. A layered linkage mechanism is adopted to start a gear motor to drive a linkage screw to rotate reversely, a concave block enables a connecting shaft to drive an inclined sleeve rod to move upwards, a sealing rotary disc is far away from an arc-shaped limiting plate along the top end of a culture disc, and the top ends of multiple culture dish groove bodies are synchronously opened; the plurality of gastrointestinal tumor stem cell culture dishes in the culture box can be quickly uncovered and checked, and the culture of the gastrointestinal tumor stem cells is more time-saving and labor-saving.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and more particularly to a cell culture device for detecting stem cells of gastrointestinal tumors. Background Art

[0002] The cell culture device for detecting gastrointestinal tumor stem cells provides an efficient platform for drug screening. Researchers can culture a large number of gastrointestinal tumor stem cells in the device, test the effects of different drugs on them, and quickly screen out drug candidates that have inhibitory effects on gastrointestinal tumor stem cells, thereby improving detection accuracy for preclinical drug development.

[0003] A search of existing public documents revealed that patent publication number CN218893688U discloses a cell culture device in which a connecting groove is provided through the top surface of the dish cover, and a partition is provided below the connecting groove. By turning a toggle block, the partition rotates within the connecting groove, connecting the connecting groove with the outside world. The user can control the rotation range of the partition according to the size of the extraction device to ensure sufficient extraction space in the dish cover, avoid directly opening the dish cover, and minimize contamination of the cell solution in the bottom of the dish by external dust and pollutants, thereby ensuring normal subsequent cell cultivation. However, this cell culture device has the following problems and defects:

[0004] When a cell culture device is used to detect gastrointestinal tumor stem cells, the gastrointestinal tumor stem cells need to be cultured, which requires placing the culture dishes inside an incubator and regularly checking the gastrointestinal tumor stem cells in each culture dish. When checking, the culture dish covers need to be opened one by one, and then the status of the gastrointestinal tumor stem cells inside the culture dishes needs to be checked one by one. This makes the gastrointestinal tumor stem cell culture more time-consuming and labor-intensive. Therefore, a cell culture device for gastrointestinal tumor stem cell detection is provided. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the present invention provides a cell culture device for detecting stem cells of gastrointestinal tumors.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solutions: A cell culture device for stem cell detection of gastrointestinal tumors, comprising a rectangular frame column and a linkage screw, wherein the linkage screw is located inside the rectangular frame column and is rotatably connected, the top end of the rectangular frame column is fixedly connected to a reduction motor for driving the linkage screw to rotate, and the outer wall of the linkage screw is provided with a layered linkage mechanism; the layered linkage mechanism comprises a plurality of sleeved sliders arranged on the outer wall of the linkage screw, one side of each sleeved slider is fixedly connected to a linkage ring, a plurality of linkage shafts are embedded in the top end of the linkage ring, and the bottom end of each linkage shaft is integrally formed with a concave block by die-casting; a connecting shaft is welded to one side of the inner wall of the concave block, and an inclined sleeve is rotatably mounted on the outer wall of the connecting shaft, a rotatably connected support shaft is passed through the inner wall of the inclined sleeve near its bottom end, a concave support block with a concave vertical cross-section is welded to one end of the support shaft, a linkage rotating plate is welded below the concave support block, and a slidably connected culture tray is mounted below the linkage rotating plate;

[0007] A displacement viewing component is installed at the bottom end of the culture dish, and multiple sleeve sliders are threadedly connected to the outer wall of the linkage screw, and multiple sleeve sliders are arranged equidistantly from top to bottom. Multiple outer walls of the sleeve sliders are vertically slidably connected to the inner wall of the rectangular frame column, and the outer wall of the sleeve slider and the inner wall of the rectangular frame column are polished and ground. Multiple linkage shafts are rotatably connected to the linkage ring, and the outer wall of the linkage shaft is set to a smooth surface.

[0008] Preferably, one end of the linkage rotating plate is fixedly connected to a sealing turntable, and an arc-shaped limiting plate fixedly connected to the culture plate is installed on one side of each of the sealing turntables, and a culture dish trough is provided inside the arc-shaped limiting plate; the other end of the linkage rotating plate is installed with a rotating ring rotatably connected to the rectangular frame column, and the inner wall of the culture plate and the outer wall of the rectangular frame column are fixedly connected, and a sliding column slidably connected to the culture plate is provided at the bottom end of the linkage rotating plate near the middle thereof, and the sliding column and the linkage rotating plate are fixedly connected.

[0009] When in use according to the above technical solution, the reduction motor drives the linkage screw to reverse, and the linkage screw causes the multiple sleeve sliders to slide up and reset along the inner wall of the rectangular frame column under the action of the thread, and the multiple sleeve sliders respectively drive the multiple linkage rings to move up synchronously, and the concave block causes the connecting shaft to drive the inclined sleeve rod to move up, and the linkage rotating plate causes the rotating ring to rotate counterclockwise along the inside of the rectangular frame column, and the sealing turntable no longer seals the top position of the culture dish trough body, and the top ends of the multiple culture dish trough bodies are opened synchronously.

[0010] Preferably, the displacement viewing assembly includes a connecting pillar fixedly mounted on the bottom end of the culture tray; a sliding chassis is fixedly mounted on the top end of the connecting pillar; a rotating rod is rotatably mounted on the bottom end of the culture tray and located on one side of the connecting pillar; a transmission motor fixedly connected to the sliding chassis is mounted on the bottom end of the rotating rod; and a rotating ring is welded concentrically to the outer wall of the rotating rod;

[0011] A rotating bar is welded to one side of the outer wall of the rotating ring, and a rotating frame plate slidably connected to the sliding chassis is installed at one end of the rotating bar, and the rotating bar and the rotating frame plate are fixedly connected. A linkage adjustment component is installed on one side of the rotating frame plate, and the bottom end of the rotating rod is coaxially connected to the output end of the transmission motor, and the center point of the rotating rod and the center point of the culture dish are on the same vertical line.

[0012] When in use according to the above technical solution, the linkage motor is started to drive the transmission screw to rotate, and the transmission screw rotates stably inside the rotating frame. The three threaded sleeves are guided down along the inner wall of the rotating frame under the action of the threads, and the threaded sleeves drive multiple inspection cameras to move downward synchronously for adjustment.

[0013] Preferably, the linkage adjustment component includes a controller fixedly mounted on one side of the rotating frame plate; a transmission screw is rotatably mounted on the inner wall of the rotating frame plate, and a linkage motor for driving the transmission screw to rotate is fixedly mounted on the top of the rotating frame plate, and a plurality of threaded sleeves are arranged equidistantly from top to bottom on the outer wall of the transmission screw, and an inspection camera is fixedly connected to the interior of each threaded sleeve located on one side of the rotating frame plate, and the plurality of threaded sleeves are connected to the transmission screw through threaded transmission, and the plurality of threaded sleeves are vertically slidably connected to the inner wall of the rotating frame plate.

[0014] When in use according to the above technical solution, the driving transmission motor drives the rotating rod to rotate. When the rotating rod rotates at the bottom of the culture dish, it can drive the rotating ring to rotate synchronously. The rotating ring drives the rotating bar to rotate the rotating frame plate. The rotating frame plate simultaneously carries the transmission screw to rotate multiple threaded sleeves. The threaded sleeves drive the inspection camera to rotate. The inspection camera can move along the top of multiple culture dish troughs. The inspection camera inspects the internal culture conditions of the gastrointestinal tumor stem cell culture dishes inside the multiple culture dish troughs.

[0015] The technical effects and advantages of the present invention are as follows:

[0016] 1. The present invention uses a layered linkage mechanism to start the reduction motor to drive the linkage screw to reverse. The linkage screw causes the multiple sleeved sliders to slide upward and reset along the inner wall of the rectangular frame column under the action of the thread. The concave block causes the connecting shaft to drive the inclined sleeve rod to move upward. The inclined sleeve rod drives the support shaft to move the concave support block upward. The sealing turntable moves away from the arc-shaped limit plate along the top of the culture dish, and the tops of the multiple culture dish tanks are opened synchronously. Multiple gastrointestinal tumor stem cell culture dishes inside the incubator can be quickly opened for inspection without having to open the covers one by one. The efficiency of the synchronous opening inspection is effectively improved, and the gastrointestinal tumor stem cell culture is more time-saving and labor-saving.

[0017] 2. The present invention uses a variable position viewing assembly to drive the transmission motor to rotate the rotating rod, and the sliding chassis provides vertical support for the bottom end of the transmission motor. The rotating ring drives the rotating bar to rotate the rotating frame plate, and the bottom end of the rotating frame plate can slide along the outer wall of the sliding chassis. The inspection camera can check the internal culture status of gastrointestinal tumor stem cell culture dishes in multiple culture dish tanks. Multi-point inspection can be carried out simultaneously without opening the incubator, which saves time and effort for gastrointestinal tumor stem cell culture inspection.

[0018] 3. The present invention uses a linkage adjustment component to enable the controller to activate the linkage motor to drive the transmission screw to rotate. The transmission screw drives the three threaded sleeves to slide down along the inner wall of the rotating frame under the action of the thread. After the vertical positions of multiple inspection cameras are adjusted synchronously, it is ensured that multiple inspection cameras can clearly view the cell status inside the gastrointestinal tumor stem cell culture dish, making the inspection more accurate and clear, and the culture inspection more time-saving and labor-saving;

[0019] The mutual influence of the above-mentioned multiple functions, first of all, the tops of multiple culture dish troughs are opened synchronously, and the vertical positions of multiple inspection cameras are adjusted synchronously. Multi-point inspection can be realized synchronously without opening the incubator. In summary, the tops of multiple gastrointestinal tumor stem cell culture dishes can be opened synchronously, and the inspection can be adjusted synchronously, which saves time and effort in gastrointestinal tumor stem cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the main structure of a cell culture device for detecting gastrointestinal tumor stem cells according to the present invention.

[0021] Figure 2 It is a schematic diagram of the partial structure of the connection between the linkage screw and the sleeve slider of the present invention.

[0022] Figure 3 It is a schematic diagram of the local structure of the connection between the linkage shaft and the concave block of the present invention.

[0023] Figure 4 For the present invention Figure 2 Enlarged structural diagram at point A in the middle.

[0024] Figure 5 This is a schematic diagram of the rear view of a cell culture device for detecting gastrointestinal tumor stem cells according to the present invention.

[0025] Figure 6 It is a schematic diagram of the local structure of the connection between the rotating rod and the transmission motor of the present invention.

[0026] Figure 7 It is a schematic diagram of the structure of the linkage adjustment component of the present invention.

[0027] The accompanying drawings are marked as follows: 1. rectangular frame column; 2. linkage screw; 3. reduction motor; 4. sleeve slider; 5. linkage ring; 6. linkage rotating shaft; 7. concave block; 8. connecting shaft; 9. tilt sleeve; 10. support shaft; 11. concave support block; 12. linkage rotating plate; 13. sealing turntable; 14. culture plate; 15. culture dish trough; 16. arc-shaped limit plate; 17. sliding column; 18. rotating ring; 19. connecting pillar; 20. sliding chassis; 21. rotating rod; 22. rotating ring; 23. transmission motor; 24. rotating bar; 25. rotating frame plate; 26. controller; 27. transmission screw; 28. threaded sleeve; 29. inspection camera; 30. linkage motor. DETAILED DESCRIPTION

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0029] As attached Figure 1-7 The cell culture device for detecting gastrointestinal tumor stem cells is shown. The device is equipped with a layered linkage mechanism, a position detection component, and a linkage adjustment component. The configuration of each mechanism and component enables the simultaneous opening and adjustment of the tops of multiple gastrointestinal tumor stem cell culture dishes, making gastrointestinal tumor stem cell culture more time-efficient and labor-saving. The specific structural configuration of each mechanism and component is as follows:

[0030] When used in this embodiment, as shown in the attached Figure 1-4As shown, the layered linkage mechanism includes a plurality of sleeved sliders 4 arranged on the outer wall of the linkage screw 2, and a linkage ring 5 is fixedly connected to one side of each sleeved slider 4, and a plurality of linkage rotating shafts 6 are embedded in the top of the linkage ring 5, and the bottom end of each linkage rotating shaft 6 is integrally formed with a concave block 7 by die-casting; a connecting shaft 8 is welded to one side of the inner wall of the concave block 7, and an inclined sleeve rod 9 is rotatably installed on the outer wall of the connecting shaft 8, and a rotatably connected support shaft 10 is passed through the inner wall of the inclined sleeve rod 9 near its bottom end, and a concave support block 11 with a concave vertical cross-section is welded to one end of the support shaft 10, and a linkage rotating plate 12 is welded below the concave support block 11, and a slidingly connected culture tray 14 is installed below the linkage rotating plate 12; a displacement viewing component is installed at the bottom end of the culture tray 14.

[0031] When used in this embodiment, as shown in the attached Figure 4 As shown, one end of the linkage rotating plate 12 is fixedly connected to the sealing turntable 13, and one side of each sealing turntable 13 is installed with an arc-shaped limiting plate 16 fixedly connected to the culture tray 14, and a culture dish trough 15 is provided inside the arc-shaped limiting plate 16; the other end of the linkage rotating plate 12 is installed with a rotating ring 18 rotatably connected to the rectangular frame column 1, and the inner wall of the culture tray 14 is fixedly connected to the outer wall of the rectangular frame column 1, and the bottom end of the linkage rotating plate 12 and near the middle position thereof are provided with a sliding column 17 slidably connected to the culture tray 14, and the sliding column 17 is fixedly connected to the linkage rotating plate 12, so that the linkage rotating plate 12 can make the sealing turntable 13 approach the arc-shaped limiting plate 16 along the top of the culture tray 14, and the sealing turntable 13 squeezes the inner wall of the arc-shaped limiting plate 16 to form a seal, which can achieve synchronous sealing of the positions of multiple culture dish troughs 15, forming a linkage operation.

[0032] When used in this embodiment, as shown in the attached Figure 4-6 As shown, the displacement viewing assembly includes a connecting pillar 19 fixedly mounted at the bottom end of the culture tray 14; a sliding chassis 20 is fixedly mounted on the top of the connecting pillar 19, and a rotating rod 21 is rotatably mounted at the bottom end of the culture tray 14 and located on one side of the connecting pillar 19. A transmission motor 23 fixedly connected to the sliding chassis 20 is mounted at the bottom end of the rotating rod 21, and a rotating ring 22 is welded concentrically to the outer wall of the rotating rod 21.

[0033] A rotating bar 24 is welded to one side of the outer wall of the rotating ring 22, and a rotating frame plate 25 slidably connected to the sliding chassis 20 is installed at one end of the rotating bar 24, and the rotating bar 24 and the rotating frame plate 25 are fixedly connected. A linkage adjustment component is installed on one side of the rotating frame plate 25, and the bottom end of the rotating rod 21 is coaxially connected to the output end of the transmission motor 23, and the center point of the rotating rod 21 and the center point of the culture dish 14 are on the same vertical line.

[0034] When used in this embodiment, as shown in the attached Figure 7As shown, the linkage adjustment component includes a controller 26 fixedly mounted on one side of the rotating frame plate 25; a transmission screw 27 is rotatably mounted on the inner wall of the rotating frame plate 25, and a linkage motor 30 for driving the transmission screw 27 to rotate is fixedly mounted on the top of the rotating frame plate 25. The outer wall of the transmission screw 27 is provided with a plurality of threaded sleeves 28 arranged equidistantly from top to bottom, and an inspection camera 29 is fixedly connected to the interior of each threaded sleeve 28 and located on one side of the rotating frame plate 25. The plurality of threaded sleeves 28 are connected to the transmission screw 27 by threaded transmission, and the plurality of threaded sleeves 28 are vertically slidably connected to the inner wall of the rotating frame plate 25.

[0035] The working principle of the cell culture device for detecting gastrointestinal tumor stem cells of the present invention is as follows:

[0036] First, when the present invention is cultured, the gastrointestinal tumor stem cell culture dish is placed in the three-layer culture tray 14, and the gastrointestinal tumor stem cell culture dish is held by the culture dish trough 15 on the culture tray 14. The reduction motor 3 is started to drive the linkage screw 2 to rotate forward. The linkage screw 2 carries multiple sleeve sliders 4 and slides down along the inner wall of the rectangular frame column 1 under the action of the thread. The multiple sleeve sliders 4 respectively drive multiple linkage rings 5 to move downward synchronously. The linkage ring 5 drives multiple linkage rotating shafts 6 to move the concave block 7 downward. The concave block 7 drives the connecting shaft 8 to drive the inclined sleeve rod 9 to move downward. The inclined sleeve rod 9 drives the support shaft 10 to move the concave support block 11 downward, and the culture tray 14 rotates the linkage rotating plate 12. The linkage rotating plate 12 drives the sliding column 17 to rotate inside the culture tray 14. The linkage rotating plate 12 causes the rotating ring 18 to rotate clockwise along the inside of the rectangular frame column 1. The linkage rotating plate 12 causes the sealing turntable 13 to move along the top of the culture tray 14 toward the arc-shaped limit plate 16. The sealing turntable 13 squeezes the inner wall of the arc-shaped limit plate 16 to form a seal, which can achieve synchronous sealing of the positions of multiple culture dish troughs 15, thereby placing the entire rectangular frame column 1 in the incubator and adjusting it to appropriate culture humidity and temperature;

[0037] Secondly, when the present invention performs distributed linkage opening, when the gastrointestinal tumor stem cell culture dish inside the incubator needs to be checked, the controller 26 can be wirelessly driven by an external device, and the controller 26 starts the reduction motor 3 to drive the linkage screw 2 to reverse, and the linkage screw 2 causes the multiple sleeve sliders 4 to slide up and reset along the inner wall guide of the rectangular frame column 1 under the action of the thread, and the multiple sleeve sliders 4 respectively drive the multiple linkage rings 5 to move up synchronously, and the concave block 7 causes the connecting shaft 8 to drive the inclined sleeve rod 9 to move up, and the inclined sleeve rod 9 drives the support shaft 10 to move the concave support block 11 upward, and the linkage rotating plate 12 causes the rotating ring 18 to rotate counterclockwise along the inside of the rectangular frame column 1, and the linkage rotating plate 12 causes the sealing turntable 13 to move away from the arc-shaped limit plate 16 along the top of the culture dish 14, and the sealing turntable 13 no longer seals the top position of the culture dish trough 15, thereby synchronously opening the top of the multiple culture dish troughs 15;

[0038] Then, when the present invention performs linkage fine adjustment, the controller 26 starts the linkage motor 30 to drive the transmission screw 27 to rotate. The transmission screw 27 rotates stably inside the rotating frame plate 25. The transmission screw 27 drives the three threaded sleeves 28 to slide down along the inner wall of the rotating frame plate 25 under the action of the thread. The multiple threaded sleeves 28 respectively drive the multiple inspection cameras 29 to move downward synchronously. After the vertical positions of the multiple inspection cameras 29 are synchronously adjusted, the inspection operation can be carried out.

[0039] Finally, when the present invention performs displacement inspection, the controller 26 is remotely started through an external remote control, so that the controller 26 drives the transmission motor 23 to drive the rotating rod 21 to rotate, and the sliding chassis 20 provides vertical support force to the bottom end of the transmission motor 23. When the rotating rod 21 rotates at the bottom end of the culture dish 14, it can drive the rotating ring 22 to rotate synchronously, and the rotating ring 22 drives the rotating bar 24 to rotate the rotating frame plate 25, and the bottom end of the rotating frame plate 25 can slide along the outer wall of the sliding chassis 20. The rotating frame plate 25 also carries the transmission screw 27 to rotate multiple threaded sleeves 28, and the threaded sleeves 28 drive the inspection camera 29 to rotate. The inspection camera 29 can move above multiple culture dish troughs 15, and the inspection camera 29 checks the internal culture conditions of the gastrointestinal tumor stem cell culture dishes inside the multiple culture dish troughs 15.

[0040] The contents not described in detail in the specification belong to the existing technology known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited, and conventional equipment can be used. In this technical solution, the electrical control components not mentioned are not shown in the figure because they belong to the existing technology and are not described here.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cell culture device for detecting stem cells of gastrointestinal tumors, comprising a rectangular frame column (1) and a linkage screw (2), wherein the linkage screw (2) is located inside the rectangular frame column (1) and is rotatably connected thereto, and a reduction motor (3) for driving the linkage screw (2) to rotate is fixedly connected to the top end of the rectangular frame column (1), characterized in that: The outer wall of the linkage screw (2) is provided with a layered linkage mechanism; The layered linkage mechanism comprises a plurality of sleeved sliders (4) arranged on the outer wall of the linkage screw (2), a linkage ring (5) is fixedly connected to one side of each sleeved slider (4), a plurality of linkage rotating shafts (6) are embedded in the top end of each linkage rotating shaft (6), and a concave block (7) is integrally formed by die-casting at the bottom end of each linkage rotating shaft (6); A connecting shaft (8) is welded to one side of the inner wall of the concave block (7), and an inclined sleeve rod (9) is rotatably mounted on the outer wall of the connecting shaft (8). A rotatably connected support shaft (10) is passed through the inner wall of the inclined sleeve rod (9) near its bottom end. A concave support block (11) having a concave vertical cross-section is welded to one end of the support shaft (10). A linkage rotating plate (12) is welded below the concave support block (11), and a slidingly connected culture tray (14) is mounted below the linkage rotating plate (12). A displacement viewing component is installed at the bottom end of the culture tray (14).

2. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 1, characterized in that: The plurality of sleeve sliding blocks (4) are all threadedly connected to the outer wall of the linkage screw rod (2), and the plurality of sleeve sliding blocks (4) are arranged in sequence and at equal distances from top to bottom.

3. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 1, characterized in that: The outer walls of the plurality of sleeve sliding blocks (4) are vertically slidably connected to the inner wall of the rectangular frame column (1), and the outer walls of the sleeve sliding blocks (4) and the inner wall of the rectangular frame column (1) are polished and ground.

4. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 1, characterized in that: The plurality of linkage rotating shafts (6) are rotationally connected to the linkage ring (5), and the outer wall of the linkage rotating shaft (6) is configured as a smooth surface.

5. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 1, characterized in that: One end of the linkage rotating plate (12) is fixedly connected to a sealing rotating disk (13), and one side of each sealing rotating disk (13) is installed with an arc-shaped limiting plate (16) fixedly connected to the culture dish (14), and a culture dish tank (15) is provided inside the arc-shaped limiting plate (16); The other end of the linkage rotating plate (12) is provided with a rotating ring (18) rotatably connected to the rectangular frame column (1), and the inner wall of the culture tray (14) is fixedly connected to the outer wall of the rectangular frame column (1). A sliding column (17) slidably connected to the culture tray (14) is provided at the bottom end of the linkage rotating plate (12) and near its center, and the sliding column (17) is fixedly connected to the linkage rotating plate (12).

6. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 1, characterized in that: The displacement viewing assembly includes a connecting pillar (19) fixedly mounted on the bottom end of the culture tray (14); A sliding chassis (20) is fixedly mounted on the top of the connecting pillar (19); a rotating rod (21) is rotatably mounted on the bottom of the culture tray (14) and located on one side of the connecting pillar (19); a transmission motor (23) fixedly connected to the sliding chassis (20) is mounted on the bottom end of the rotating rod (21); and a rotating ring (22) is welded cocentrically to the outer wall of the rotating rod (21); A rotating bar (24) is welded to one side of the outer wall of the rotating ring (22); a rotating frame plate (25) slidably connected to the sliding chassis (20) is installed at one end of the rotating bar (24); the rotating bar (24) and the rotating frame plate (25) are fixedly connected; and a linkage adjustment component is installed on one side of the rotating frame plate (25).

7. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 6, characterized in that: The bottom end of the rotating rod (21) is coaxially connected to the output end of the transmission motor (23), and the center point of the rotating rod (21) and the center point of the culture plate (14) are located on the same vertical line.

8. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 6, characterized in that: The linkage adjustment assembly includes a controller (26) fixedly mounted on one side of the rotating frame plate (25); A transmission screw (27) is rotatably mounted on the inner wall of the rotating frame plate (25), and a linkage motor (30) for driving the transmission screw (27) to rotate is fixedly mounted on the top of the rotating frame plate (25). A plurality of threaded sleeves (28) are equidistantly arranged on the outer wall of the transmission screw (27) from top to bottom, and an inspection camera (29) is fixedly connected to the inside of each threaded sleeve (28) and located on one side of the rotating frame plate (25).

9. The cell culture device for detecting gastrointestinal tumor stem cells according to claim 8, characterized in that: The plurality of threaded sleeves (28) are all connected to the transmission screw (27) via threaded transmission, and the plurality of threaded sleeves (28) are all vertically slidably connected to the inner wall of the rotating frame plate (25).

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