Tracheal catheter with antibacterial and lubricating functions and preparation method thereof

Abstract

The invention relates to the technical field of biomedical materials, and particularly discloses a tracheal catheter with antibacterial and lubricating functions and a preparation method thereof.According to the tracheal catheter, 2, 3-epoxypropyltrimethylammonium chloride is adopted for modifying polyethyleneimine, and the antibacterial performance of the polyethyleneimine is enhanced; then modified polyethyleneimine and tannic acid are adopted as a composite antibacterial coating, so that the tracheal catheter has good antibacterial activity on various bacteria; then N-vinyl pyrrolidone and modified fucoidin are adopted as functional monomers, ultraviolet-initiated polymerization is conducted, the hydrophilic lubricating coating is obtained, sulfuric acid groups in the hydrophilic lubricating coating are negatively charged and can attract quaternary ammonium salt cations in the antibacterial coating through electrostatic interaction, and therefore the hydrophilic lubricating coating is formed. Compared with a single polyvinylpyrrolidone (PVP) hydrophilic coating which is easy to fall off, the hydrophilic lubricating coating disclosed by the invention effectively solves the problem that the traditional hydrophilic coating is easy to fall off.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical materials, and in particular to a tracheal tube with antibacterial and lubricating functions and a preparation method thereof. Background Art

[0002] Endotracheal intubation is an important first aid and treatment method. By inserting a medical endotracheal tube into the airway through the mouth or nose, the patient's airway can be kept open and mechanical ventilation can be performed. This technology is widely used in clinical treatments such as cardiopulmonary resuscitation, respiratory medicine, and anesthesia, and can be used to treat respiratory failure, airway obstruction, loss of consciousness, and other conditions. Currently, common endotracheal tubes in clinical practice are mainly made of polymer materials such as silicone rubber, polyvinyl chloride (PVC), and polydimethylsiloxane (PDMS). These materials are widely used in the preparation of medical consumables and medical devices due to their advantages such as easy processing, high toughness, and high stability.

[0003] Endotracheal intubation is widely used in clinical practice, but currently used endotracheal tubes still lack antibacterial and lubricity. The mechanical properties of traditional endotracheal tube materials are not compatible with those of human soft tissue, resulting in high friction during intubation, which can easily cause patients to experience severe discomfort, such as coughing, sore throat, and other stress reactions. This friction can also cause edema or inflammation of the airway lining, further leading to problems such as dysphonia and dysphagia. Furthermore, traditional catheters lack antibacterial properties, allowing bacteria and other organisms to easily adhere to the catheter surface, forming biofilms and contaminating the catheter. This can not only cause local infection but can also spread pathogens, leading to systemic infection and increasing the risk of ventilator-associated pneumonia (VAP).

[0004] Regarding lubricity, while lubricants can reduce airway irritation caused by intubation to a certain extent, traditional lubricants, such as lidocaine gel, only adhere to the tube surface for a short time, resulting in limited lubrication. During intubation, the lubricant is gradually consumed due to contact and friction between the tube and surrounding tissue, resulting in a weakened lubricating effect. Furthermore, residual lubricant may also affect postoperative airway function. In recent years, the application of super-lubricant coating technology has improved the lubricity of endotracheal tubes to a certain extent, but the adhesion and durability of this coating still need to be improved. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the object of the present invention is to provide an endotracheal tube with antibacterial and lubricating functions and a preparation method thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a method for preparing an endotracheal tube with antibacterial lubrication function, comprising the following steps:

[0008] S1. Preparation of pre-treated endotracheal tube

[0009] The tracheal tube to be treated is placed in an oxygen plasma instrument and activated to obtain a pretreated tracheal tube.

[0010] In this step, the material of the tracheal tube is selected from polyvinyl chloride, silicone rubber or polydimethylsiloxane.

[0011] In this step, the gas flow rate of the oxygen plasma treatment is 100-150 sccm, for example, 100 sccm, 105 sccm, 110 sccm, 115 sccm, 120 sccm, 125 sccm, 130 sccm, 135 sccm, 140 sccm, 145 sccm, and 150 sccm can be selected; the treatment power is 100-300 W, for example, 100 W, 150 W, 200 W, 250 W, and 300 W can be selected; the treatment time is 3-10 min, for example, 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, 9 min, and 10 min can be selected; the treatment pressure is 10-100 Pa, for example, 10 Pa, 20 Pa, 30 Pa, 40 Pa, 50 Pa, 60 Pa, 70 Pa, 80 Pa, 90 Pa, and 100 Pa can be selected, but are not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0012] In this step, the surface of the material used for the endotracheal tube is mostly highly hydrophobic. The present invention uses oxygen plasma to treat the surface of the material to introduce oxygen-containing groups such as hydroxyl and carboxyl groups on the surface of the material. The increase of these polar groups makes the surface of the material hydrophilic and also enhances the bonding strength with the coating.

[0013] S2. Preparation of modified polyethyleneimine

[0014] Polyethyleneimine and 2,3-epoxypropyltrimethylammonium chloride are added to deionized water, heated and stirred for reaction, and after the reaction is completed, dialyzed and freeze-dried to obtain modified polyethyleneimine.

[0015] In this step, the mass ratio of polyethyleneimine to 2,3-epoxypropyltrimethylammonium chloride is 5-10:8-12, for example, 5:8, 5:10, 5:12, 6:10, 8:8, 8:10, 8:12, 10:8, 10:11, 10:12 can be selected, but is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0016] In this step, the temperature of the heating and stirring reaction is 70-85°C, for example, 70°C, 75°C, 80°C, or 85°C can be selected; the reaction time is 8-16h, for example, 8h, 10h, 12h, 14h, 15h, or 16h can be selected, but is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0017] In this step, dialysis was performed using a dialysis membrane with a molecular weight cutoff of 1000 Da to remove unreacted 2,3-epoxypropyltrimethylammonium chloride.

[0018] Polyethyleneimine has abundant amino groups (primary amines, secondary amines, and tertiary amines), which can chemically bond with polar groups (such as carboxyl groups, hydroxyl groups, etc.) on the surface of the substrate. For example, the amino groups can form amide bonds with carboxyl groups and hydrogen bonds with hydroxyl groups, thereby enhancing the adhesion between PEI and the substrate. In the present invention, 2,3-epoxypropyltrimethylammonium chloride is used to modify polyethyleneimine, thereby enhancing the antibacterial properties of polyethyleneimine.

[0019] S3. Preparation of antibacterial modified endotracheal tube

[0020] The modified polyethyleneimine and tannic acid are added into deionized water, mixed evenly, then immersed into a pretreated tracheal tube, soaked, taken out and solidified to obtain an antibacterial modified tracheal tube.

[0021] In this step, the mass ratio of modified polyethyleneimine, tannic acid and deionized water is 1-3:0.5-1.5:100, for example, 1:0.5:100, 1:1:100, 1:1.5:100, 2:0.5:100, 2:1:100, 2:1.5:100, 3:0.5:100, 3:1:100, and 3:1.5:100 can be selected, but is not limited to the listed values. Other values ​​not listed within the numerical range are also applicable.

[0022] In this step, the curing temperature is 100-120°C, for example, 100°C, 105°C, 110°C, 115°C, and 120°C can be selected; the curing time is 1-2h, for example, 1h, 1.5h, and 2h can be selected, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0023] The present invention uses modified polyethyleneimine and tannic acid as a composite antibacterial coating, which makes the endotracheal tube have good antibacterial activity against a variety of bacteria and can effectively inhibit the attachment and growth of bacteria. At the same time, tannic acid also has good adhesion properties, allowing the composite antibacterial coating to firmly adhere to various substrates, ensuring that the coating has long-lasting antibacterial properties.

[0024] S4. Preparation of endotracheal tube with antibacterial lubrication function

[0025] N-vinyl pyrrolidone and modified fucoidan are added to an ethanol aqueous solution, and then an initiator is added and mixed evenly to obtain a coating liquid. An antibacterial modified tracheal tube is immersed in the coating liquid, then taken out and subjected to ultraviolet cross-linking under nitrogen protection. The tube is then washed and dried to obtain a tracheal tube with antibacterial lubricating function.

[0026] In this step, the mass ratio of N-vinyl pyrrolidone, modified fucoidan, ethanol aqueous solution and initiator is 10-15:3-6:100:0.5-1.

[0027] Specifically, the initiator is selected from benzophenone, 3-methylbenzophenone or 4-methylbenzophenone.

[0028] Specifically, the preparation method of the modified fucoidan is as follows: dissolving fucoidan in deionized water to obtain an aqueous phase; dissolving acryloyl chloride and triethylamine in dichloromethane to obtain an organic phase; under nitrogen protection, adding the organic phase dropwise to the aqueous phase, stirring to react, and after the reaction is completed, precipitating, washing, vacuum drying, and grinding to obtain the modified fucoidan.

[0029] More specifically, the mass ratio of fucoidan, acryloyl chloride and triethylamine is 5-10:4-6:2-3.

[0030] More specifically, the stirring reaction is carried out at room temperature for 4-8 hours.

[0031] In this step, when UV cross-linking is performed, the UV light intensity is 150-200 mW / cm 2 , UV cross-linking time is 90-120s.

[0032] In this step, fucoidan is a natural water-soluble polysaccharide extracted from brown algae, mainly composed of L-fucose and sulfate groups. The present invention first modifies it to introduce double bonds, and then uses N-vinyl pyrrolidone and modified fucoidan as functional monomers. After ultraviolet-initiated polymerization, a hydrophilic lubricating coating is obtained. The sulfate groups in the hydrophilic lubricating coating are negatively charged and can attract the quaternary ammonium salt cations in the antibacterial coating through electrostatic interaction, so that the hydrophilic lubricating coating is firmly fixed to the surface of the tracheal tube. Compared with a single polyvinyl pyrrolidone (PVP) hydrophilic coating that is easy to fall off, the present invention effectively solves the problem of easy falling off of the hydrophilic coating. At the same time, the fucoidan provided by the present invention also has good anti-protein adhesion properties, and works together with N-vinyl pyrrolidone to improve the anti-protein adhesion effect of the tracheal tube.

[0033] The present invention also provides a tracheal tube with antibacterial and lubricating functions prepared by the above preparation method.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] (1) The present invention treats the surface of the material by using oxygen plasma to introduce oxygen-containing groups such as hydroxyl and carboxyl groups on the surface of the material. The increase of these polar groups makes the surface of the material hydrophilic and also enhances the bonding strength with the coating.

[0036] (2) The present invention first uses 2,3-epoxypropyltrimethylammonium chloride to modify polyethyleneimine, thereby enhancing the antibacterial properties of polyethyleneimine; then, the modified polyethyleneimine and tannic acid are used as a composite antibacterial coating, so that the tracheal tube has good antibacterial activity against a variety of bacteria and can effectively inhibit the attachment and growth of bacteria; at the same time, tannic acid also has good adhesion properties, so that the composite antibacterial coating can be firmly attached to various substrates, ensuring that the coating has long-lasting antibacterial properties.

[0037] (3) Fucoidan is a natural water-soluble polysaccharide extracted from brown algae, mainly composed of L-fucose and sulfate groups. The present invention first modifies the fucoidan to introduce double bonds, then uses N-vinyl pyrrolidone and modified fucoidan as functional monomers, and obtains a hydrophilic lubricating coating after ultraviolet initiation polymerization. The cross-linked hydrophilic lubricating coating is a hydrogel with an interpenetrating network structure. This structure can absorb and retain moisture in a wet environment, forming a stable lubricating layer on the surface, which can maintain low friction even after repeated friction and long-term use. The friction coefficient is low, and the sulfate groups in the hydrophilic lubricating coating are negatively charged, which can attract the quaternary ammonium salt cations in the antibacterial coating through electrostatic interaction, so that the hydrophilic lubricating coating is firmly fixed on the surface of the tracheal tube. Compared with the single polyvinyl pyrrolidone (PVP) hydrophilic coating that is easy to fall off, the present invention effectively solves the problem of the traditional hydrophilic coating that is easy to fall off and has a better lubricating effect. At the same time, the fucoidan provided by the present invention also has good anti-protein adhesion performance, and works together with N-vinyl pyrrolidone to improve the anti-protein adhesion effect of the tracheal tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 The graphs show the antibacterial performance test results of different groups;

[0039] Figure 2 Figure 2 is the friction performance test result diagram of different groups;

[0040] Figure 3 Graph showing the test results of anti-protein adhesion performance of different groups. DETAILED DESCRIPTION

[0041] The present invention is further described in detail below through specific preferred embodiments, but the present invention is not limited to the following embodiments.

[0042] It should be noted that, unless otherwise specified, all chemical reagents involved in the present invention were purchased through commercial channels.

[0043] The endotracheal tube used in the embodiment of the present invention is made of PVC; the average relative molecular mass of polyethyleneimine is 3000.

[0044] Example 1

[0045] A method for preparing an endotracheal tube with antibacterial lubrication function comprises the following steps:

[0046] S1. Place the endotracheal tube to be treated in an oxygen plasma instrument for activation treatment. The oxygen plasma treatment gas flow rate is 100 seem, the treatment power is 150 W, the treatment time is 5 minutes, and the treatment pressure is 50 Pa to obtain a pretreated endotracheal tube.

[0047] S2. Add 5 g of polyethyleneimine and 8 g of 2,3-epoxypropyltrimethylammonium chloride to 100 mL of deionized water, heat and stir at 85° C. to react for 8 h. After the reaction is completed, dialyze using a dialysis membrane with a molecular weight cutoff of 1000 Da for 24 h to remove unreacted 2,3-epoxypropyltrimethylammonium chloride, and then freeze-dry to obtain modified polyethyleneimine;

[0048] S3. Add 1 g of modified polyethyleneimine and 0.5 g of tannic acid to 100 mL of deionized water, mix well, and then immerse the pretreated endotracheal tube in the mixture for 5 minutes. After removal, cure the mixture at 120° C. for 1 hour to obtain an antibacterial modified endotracheal tube.

[0049] S4. Add 10 g of N-vinyl pyrrolidone and 3 g of modified fucoidan to 100 g of a 40 wt% ethanol aqueous solution, then add 0.5 g of benzophenone and mix well to obtain a coating liquid. Immerse the antibacterial modified endotracheal tube in the coating liquid and remove it after 5 minutes. Perform UV crosslinking under nitrogen protection at a UV light intensity of 150 mW / cm 2 The UV cross-linking time is 120s, and then after washing and drying, a tracheal tube with antibacterial lubricating function is obtained;

[0050] Among them, the preparation method of modified fucoidan is as follows: 5g of fucoidan is dissolved in 50mL of deionized water to obtain an aqueous phase; 4g of acryloyl chloride and 2g of triethylamine are dissolved in 20mL of dichloromethane to obtain an organic phase; under nitrogen protection, the organic phase is added dropwise to the aqueous phase, and the reaction is stirred at room temperature for 6h. After the reaction is completed, precipitation, washing, vacuum drying, and grinding are carried out to obtain modified fucoidan.

[0051] Example 2

[0052] A method for preparing an endotracheal tube with antibacterial lubrication function comprises the following steps:

[0053] S1. Place the endotracheal tube to be treated in an oxygen plasma instrument for activation treatment. The oxygen plasma treatment gas flow rate is 150 seem, the treatment power is 100 W, the treatment time is 10 minutes, and the treatment pressure is 50 Pa to obtain a pretreated endotracheal tube.

[0054] S2. Add 10 g of polyethyleneimine and 12 g of 2,3-epoxypropyltrimethylammonium chloride to 100 mL of deionized water, heat and stir at 70° C. to react for 16 h. After the reaction is completed, dialyze using a dialysis membrane with a molecular weight cutoff of 1000 Da for 24 h to remove unreacted 2,3-epoxypropyltrimethylammonium chloride, and then freeze-dry to obtain modified polyethyleneimine;

[0055] S3. Add 1 g of modified polyethyleneimine and 1 g of tannic acid to 100 mL of deionized water, mix well, and then immerse the pretreated endotracheal tube in the mixture for 5 minutes. After removal, cure the mixture at 120° C. for 1 hour to obtain an antibacterial modified endotracheal tube.

[0056] S4. Add 15 g of N-vinyl pyrrolidone and 6 g of modified fucoidan to 100 g of a 40 wt% ethanol aqueous solution, then add 1 g of benzophenone and mix well to obtain a coating solution. Immerse the antibacterial modified endotracheal tube in the coating solution and remove it after 5 minutes. Perform UV crosslinking under nitrogen protection at a UV light intensity of 200 mW / cm 2 , the UV cross-linking time is 90s, and then after washing and drying, a tracheal tube with antibacterial lubricating function is obtained;

[0057] Among them, the preparation method of modified fucoidan is as follows: 5g of fucoidan is dissolved in 50mL of deionized water to obtain an aqueous phase; 4g of acryloyl chloride and 2g of triethylamine are dissolved in 20mL of dichloromethane to obtain an organic phase; under nitrogen protection, the organic phase is added dropwise to the aqueous phase, and the reaction is stirred at room temperature for 6h. After the reaction is completed, precipitation, washing, vacuum drying, and grinding are carried out to obtain modified fucoidan.

[0058] Example 3

[0059] A method for preparing an endotracheal tube with antibacterial lubrication function comprises the following steps:

[0060] S1. Place the endotracheal tube to be treated in an oxygen plasma instrument for activation treatment. The oxygen plasma treatment gas flow rate is 100 seem, the treatment power is 300 W, the treatment time is 3 minutes, and the treatment pressure is 50 Pa to obtain a pretreated endotracheal tube.

[0061] S2, adding 8 g of polyethyleneimine and 10 g of 2,3-epoxypropyltrimethylammonium chloride to 100 mL of deionized water, heating and stirring at 80° C. to react for 12 h. After the reaction is completed, dialyzing is performed using a dialysis membrane with a molecular weight cutoff of 1000 Da for 24 h to remove unreacted 2,3-epoxypropyltrimethylammonium chloride, and then freeze-drying to obtain modified polyethyleneimine;

[0062] S3. Add 3 g of modified polyethyleneimine and 1.5 g of tannic acid to 100 mL of deionized water, mix well, and then immerse the pretreated endotracheal tube in the mixture for 5 minutes. After removal, cure the mixture at 120° C. for 1 hour to obtain an antibacterial modified endotracheal tube.

[0063] S4. Add 12 g of N-vinyl pyrrolidone and 4 g of modified fucoidan to 100 g of a 40 wt% ethanol aqueous solution, then add 0.8 g of benzophenone and mix well to obtain a coating solution. Immerse the antibacterial modified endotracheal tube in the coating solution and remove it after 5 minutes. Perform UV crosslinking under nitrogen protection at a UV light intensity of 200 mW / cm 2 , the UV cross-linking time is 90s, and then after washing and drying, a tracheal tube with antibacterial lubricating function is obtained;

[0064] Among them, the preparation method of modified fucoidan is as follows: dissolve 10g of fucoidan in 100mL of deionized water to obtain an aqueous phase; dissolve 6g of acryloyl chloride and 3g of triethylamine in 40mL of dichloromethane to obtain an organic phase; under nitrogen protection, add the organic phase dropwise to the aqueous phase, stir and react at room temperature for 8h, and after the reaction is completed, precipitate, wash, vacuum dry, and grind to obtain modified fucoidan.

[0065] Comparative Example 1

[0066] A method for preparing an endotracheal tube with antibacterial lubrication function comprises the following steps:

[0067] S1. Place the endotracheal tube to be treated in an oxygen plasma instrument for activation treatment. The oxygen plasma treatment gas flow rate is 100 seem, the treatment power is 150 W, the treatment time is 5 minutes, and the treatment pressure is 50 Pa to obtain a pretreated endotracheal tube.

[0068] S2. Add 1 g of polyethyleneimine and 0.5 g of tannic acid to 100 mL of deionized water, mix well, and then immerse the pretreated endotracheal tube in the water for 5 minutes. After removal, cure the tube at 120° C. for 1 hour to obtain an antibacterial modified endotracheal tube.

[0069] S3. Add 10 g of N-vinyl pyrrolidone and 3 g of modified fucoidan to 100 g of a 40 wt% ethanol aqueous solution, then add 0.5 g of benzophenone and mix well to obtain a coating solution. Immerse the antibacterial modified endotracheal tube in the coating solution and remove it after 5 minutes. Perform UV crosslinking under nitrogen protection at a UV light intensity of 150 mW / cm 2 The UV cross-linking time is 120s, and then after washing and drying, a tracheal tube with antibacterial lubricating function is obtained;

[0070] Among them, the preparation method of modified fucoidan is as follows: 5g of fucoidan is dissolved in 50mL of deionized water to obtain an aqueous phase; 4g of acryloyl chloride and 2g of triethylamine are dissolved in 20mL of dichloromethane to obtain an organic phase; under nitrogen protection, the organic phase is added dropwise to the aqueous phase, and the reaction is stirred at room temperature for 6h. After the reaction is completed, precipitation, washing, vacuum drying, and grinding are carried out to obtain modified fucoidan.

[0071] Compared with Example 1, Comparative Example 1 did not perform modification treatment on the polyethyleneimine.

[0072] Comparative Example 2

[0073] A method for preparing an endotracheal tube with antibacterial lubrication function comprises the following steps:

[0074] S1. Place the endotracheal tube to be treated in an oxygen plasma instrument for activation treatment. The oxygen plasma treatment gas flow rate is 100 seem, the treatment power is 150 W, the treatment time is 5 minutes, and the treatment pressure is 50 Pa to obtain a pretreated endotracheal tube.

[0075] S2. Add 5 g of polyethyleneimine and 8 g of 2,3-epoxypropyltrimethylammonium chloride to 100 mL of deionized water, heat and stir at 85° C. to react for 8 h. After the reaction is completed, dialyze using a dialysis membrane with a molecular weight cutoff of 1000 Da for 24 h to remove unreacted 2,3-epoxypropyltrimethylammonium chloride, and then freeze-dry to obtain modified polyethyleneimine;

[0076] S3. Add 1 g of modified polyethyleneimine and 0.5 g of tannic acid to 100 mL of deionized water, mix well, and then immerse the pretreated endotracheal tube in the mixture for 5 minutes. After removal, cure the mixture at 120° C. for 1 hour to obtain an antibacterial modified endotracheal tube.

[0077] S4. Add 10 g of N-vinyl pyrrolidone to 100 g of a 40 wt% ethanol aqueous solution, then add 0.5 g of benzophenone, mix well, and obtain a coating liquid. Immerse the antibacterial modified endotracheal tube in the coating liquid, remove it after 5 minutes, and perform UV crosslinking under nitrogen protection at a UV light intensity of 150 mW / cm2 The UV cross-linking time is 120s, and then after washing and drying, a tracheal tube with antibacterial and lubricating functions is obtained.

[0078] Compared with Example 1, Comparative Example 2 did not add fucoidan.

[0079] Comparative Example 3

[0080] A method for preparing an endotracheal tube with antibacterial lubrication function comprises the following steps:

[0081] S1. Place the endotracheal tube to be treated in an oxygen plasma instrument for activation treatment. The oxygen plasma treatment gas flow rate is 100 seem, the treatment power is 150 W, the treatment time is 5 minutes, and the treatment pressure is 50 Pa to obtain a pretreated endotracheal tube.

[0082] S2. Add 5 g of polyethyleneimine and 8 g of 2,3-epoxypropyltrimethylammonium chloride to 100 mL of deionized water, heat and stir at 85° C. to react for 8 h. After the reaction is completed, dialyze using a dialysis membrane with a molecular weight cutoff of 1000 Da for 24 h to remove unreacted 2,3-epoxypropyltrimethylammonium chloride, and then freeze-dry to obtain modified polyethyleneimine;

[0083] S3. Add 1 g of modified polyethyleneimine and 0.5 g of tannic acid to 100 mL of deionized water, mix well, and then immerse the pretreated endotracheal tube in the mixture for 5 minutes. After removal, cure the mixture at 120° C. for 1 hour to obtain an antibacterial modified endotracheal tube.

[0084] S4. Add 10 g of N-vinyl pyrrolidone and 3 g of fucoidan to 100 g of a 40 wt% ethanol aqueous solution, then add 0.5 g of benzophenone and mix well to obtain a coating liquid. Immerse the antibacterial modified endotracheal tube in the coating liquid and remove it after 5 minutes. Perform UV crosslinking under nitrogen protection at a UV light intensity of 150 mW / cm 2 The UV cross-linking time is 120s, and then after washing and drying, a tracheal tube with antibacterial and lubricating functions is obtained.

[0085] Compared with Example 1, in Comparative Example 3, no modification treatment was performed on the fucoidan.

[0086] The performance of the tracheal tube samples prepared in Example 1 and Comparative Examples 1-3 was tested as follows:

[0087] Antibacterial performance test

[0088] The tracheal tube samples prepared in Example 1 and Comparative Examples 1-3 were cut into pieces to make samples of 0.5 cm × 0.5 cm, and placed in one well of a 24-well multi-well culture plate to fully expose the inner surface. 6 CFU / mL) by 3cm 2 / ml ratio was dropped on the sample surface, and then incubated at 37 ° C for 24 hours. The bacterial suspension incubated with the experimental material was diluted 1000 times, and then 50 μL of the diluted bacterial suspension was evenly applied on the solid bacterial culture (NA) plate, incubated at 37 ° C for 24 hours, and the number of bacteria was counted to calculate the antibacterial rate. The test was repeated 3 times and the results were averaged. The test results are shown as follows: Figure 1 As shown, from Figure 1 It can be seen that in Comparative Example 1, the polyethyleneimine was not modified and the antibacterial performance was significantly reduced.

[0089] Friction performance test

[0090] The lubrication properties of the surface coatings of the samples prepared in Example 1 and Comparative Examples 1-3 were tested using a pin-on-disc friction tester. The friction pairs were the prepared samples (0.5 cm × 0.5 cm) and a steel ball (6 mm in diameter). The friction coefficient was measured by linear reciprocating motion in deionized water at room temperature. The applied load was 5 N, the sliding speed was set to 5 mm / s, and the test was repeated three times. The results were averaged.

[0091] Durability test: The samples prepared in Example 1 and Comparative Examples 1-3 were immersed in deionized water at 37°C for 7 days, dried, and then subjected to friction performance test according to the above method. The test results are as follows: Figure 2 As shown, from Figure 2 It can be seen that before immersion treatment, since the surfaces of the samples of Example 1 and Comparative Examples 1-3 are all covered with a hydrophilic coating, the difference in friction coefficient is not obvious; but after immersion treatment, the hydrophilic coating on the surface of the samples prepared in Comparative Examples 1-3 begins to fall off, resulting in a significant increase in the friction coefficient. The coating prepared in the embodiments of the present invention has good stability.

[0092] Anti-protein adhesion performance test

[0093] The samples prepared in Example 1 and Comparative Examples 1-3 were incubated with 0.1 mg / mL fibrinogen at 37°C for 2 h. After rinsing with PBS and ultrasonic elution of the adhered protein using a 2 wt% sodium dodecyl sulfate solution, the protein concentration was measured using a BCA protein concentration assay kit, and the amount of protein adhered was calculated. The test was repeated three times, and the results were averaged.

[0094] The samples prepared in Example 1 and Comparative Examples 1-3 were immersed in deionized water at 37° C. for 7 days, dried, and then subjected to the protein adhesion resistance test according to the above method.

[0095] The test results are as follows Figure 3 As shown, from Figure 3 It can be seen that in Comparative Example 2, no fucoidan was added, and its anti-protein adhesion performance was significantly reduced. Compared with Comparative Examples 1 and 3, after soaking treatment, the sample prepared in the embodiment of the present invention still had good anti-protein adhesion performance.

[0096] Finally, it should be noted that the above embodiments do not limit the present invention in any form. Those skilled in the art will appreciate that modifications and improvements can be made based on the present invention. Therefore, any modifications or improvements made without departing from the spirit of the present invention are intended to fall within the scope of protection claimed in the present invention.

Claims

1. A method for preparing a tracheal tube with antibacterial lubrication function, characterized in that: The steps include: S1. placing the tracheal tube to be treated in an oxygen plasma instrument for activation treatment to obtain a pretreated tracheal tube; S2, adding polyethyleneimine and 2,3-epoxypropyltrimethylammonium chloride to deionized water, heating and stirring to react, and after the reaction is completed, dialyzing and freeze-drying to obtain modified polyethyleneimine; S3, adding modified polyethyleneimine and tannic acid to deionized water, mixing evenly, then immersing the pretreated endotracheal tube in the water, soaking the tube, taking it out and curing it to obtain an antibacterial modified endotracheal tube; S4. Add N-vinyl pyrrolidone and modified fucoidan to an ethanol aqueous solution, then add an initiator and mix well to obtain a coating liquid. Immerse the antibacterial modified endotracheal tube in the coating liquid, then take it out and perform ultraviolet cross-linking under nitrogen protection. Then, wash and dry the endotracheal tube with antibacterial lubricating function.

2. The preparation method according to claim 1, characterized in that In step S1 , the gas flow rate of the oxygen plasma treatment is 100-150 sccm, the treatment power is 100-300 W, the treatment time is 3-10 min, and the treatment pressure is 10-100 Pa.

3. The preparation method according to claim 1, characterized in that In step S2, the mass ratio of polyethyleneimine to 2,3-epoxypropyltrimethylammonium chloride is 5-10:8-12.

4. The preparation method according to claim 1, characterized in that In step S2, the temperature of the heating and stirring reaction is 70-85° C., and the reaction time is 8-16 hours.

5. The preparation method according to claim 1, characterized in that In step S3, the mass ratio of modified polyethyleneimine, tannic acid and deionized water is 1-3:0.5-1.5:

100.

6. The preparation method according to claim 1, characterized in that In step S4, the mass ratio of N-vinyl pyrrolidone, modified fucoidan, ethanol aqueous solution and initiator is 10-15:3-6:100:0.5-1, wherein the initiator is selected from benzophenone, 3-methylbenzophenone or 4-methylbenzophenone.

7. The preparation method according to claim 1, characterized in that In step S4, the preparation method of the modified fucoidan is as follows: dissolving fucoidan in deionized water to obtain an aqueous phase; dissolving acryloyl chloride and triethylamine in dichloromethane to obtain an organic phase; under nitrogen protection, adding the organic phase dropwise to the aqueous phase, stirring to react, and after the reaction is completed, precipitating, washing, vacuum drying, and grinding to obtain the modified fucoidan.

8. The preparation method according to claim 7, characterized in that The mass ratio of fucoidan, acryloyl chloride and triethylamine is 5-10:4-6:2-3.

9. The preparation method according to claim 1, characterized in that In step S4, the UV light intensity is 150-200 mW / cm 2 , UV cross-linking time is 90-120s.

10. The endotracheal tube with antibacterial and lubricating function prepared by the preparation method according to any one of claims 1 to 9.

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