Ecological-friendly leaf surface blocking and controlling agent aiming at arsenic pollution of rice and preparation method of ecological-friendly leaf surface blocking and controlling agent

Abstract

The invention discloses an eco-friendly leaf surface blocking and controlling agent aiming at arsenic pollution of rice and a preparation method thereof. According to the invention, the potassium glutamate, the S-allyl-L-cysteine and the o-hydroxypiperidine acid are combined for use, so that the growth and the stress resistance of the rice are positively influenced. Potassium element in potassium glutamate participates in activation of various enzymes, so that photosynthesis and protein synthesis are promoted, and growth and development of rice are facilitated. The S-allyl-L-cysteine not only has the function of complexing arsenic ions, but also can be used as an antioxidant to scavenge free radicals, relieve oxidative stress reaction caused by arsenic and protect rice cells from being damaged. The o-hydroxypiperidine acid not only enhances the complexing effect, but also possibly improves the adaptability of the rice to arsenic pollution by influencing the ion balance in the rice body, optimizing the microenvironment in cells and improving the adaptability of the rice to arsenic pollution, so that the absorption and accumulation of the rice to arsenic are effectively reduced, and the overall health level of the rice is enhanced by improving the growth condition and physiological status of the rice.

Description

Technical Field

[0001] The invention belongs to the technical field of rice cultivation and production, and in particular relates to an eco-friendly foliar barrier agent for arsenic pollution of rice and a preparation method thereof. Background Art

[0002] With the rapid development of modern industry and the intensification of agricultural production, the problem of soil arsenic contamination is becoming increasingly serious and has become one of the major issues threatening agricultural production safety and the ecological environment. As one of the world's major food crops, rice has a high absorption capacity for arsenic, resulting in excessive arsenic content in rice, which seriously affects human health. At present, the main methods for controlling arsenic contamination in rice include soil improvement, water management, and planting arsenic-resistant varieties. However, these methods have problems such as long control cycles, limited effects, and high costs. As a control method that directly acts on rice leaves, foliar barrier agents have the advantages of being fast, efficient, and simple, and have become a research hotspot.

[0003] However, most existing foliar inhibitors contain synthetic chemicals, which may pose potential risks to the environment and human health. Therefore, developing an eco-friendly, efficient, and safe foliar inhibitor for arsenic contamination in rice and its preparation method is of great significance for ensuring safe rice production and protecting the ecological environment. Summary of the Invention

[0004] The purpose of the present invention is to provide an eco-friendly foliar control agent for arsenic pollution of rice and a preparation method thereof in order to solve the above-mentioned problems.

[0005] The technical solution adopted by the present invention is as follows: an eco-friendly foliar control agent for arsenic contamination of rice, characterized by comprising:

[0006] Potassium glutamate: 5-10 parts by weight; S-allyl-L-cysteine: 1-3 parts by weight; o-hydroxypiperidinic acid: 1-3 parts by weight; seaweed extract: 3-7 parts by weight; licorice extract: 3-7 parts by weight; astragalus extract: 3-7 parts by weight; isatis root extract: 3-7 parts by weight; citric acid: 1-5 parts by weight; tea saponin: 1-5 parts by weight; gum arabic: 1-5 parts by weight; xanthan gum: 1-5 parts by weight; silicate: 2-5 parts by weight; calcium magnesium fertilizer: 1-3 parts by weight.

[0007] Plant growth regulators: Gibberellic acid: 0.1-0.5 parts by weight; Paclobutrazol: 0.1-0.5 parts by weight;

[0008] Plant ingredients: pepper extract: 0.5-1 parts by weight; datura flower extract: 0.5-1 parts by weight; purified water: 70-80 parts by weight.

[0009] In a preferred embodiment, the preparation method comprises the following steps:

[0010] S1: Use an electronic balance to accurately weigh potassium glutamate, place it in a clean beaker, add distilled water, and stir until the potassium glutamate is completely dissolved;

[0011] S2: Accurately weigh S-allyl-L-cysteine ​​using an electronic balance, add it to the potassium glutamate solution, stir evenly using a stirring tool, and let it stand for 10 minutes to allow the S-allyl-L-cysteine ​​to fully dissolve;

[0012] S3: Weigh 1-3 parts by weight of o-hydroxypiperidinic acid, add it to the solution prepared in step S2, stir evenly, and let it stand for 10 minutes;

[0013] S4: Add seaweed extract, licorice extract, astragalus extract, isatis root extract, citric acid, tea saponin, gum arabic, xanthan gum, silicate, and calcium magnesium fertilizer in sequence, stirring evenly after adding each ingredient;

[0014] S5: Use an electronic balance to accurately weigh gibberellin, add it to the solution prepared in step S4, stir evenly using a stirring tool, and let it stand for 10 minutes to allow the gibberellin to fully dissolve;

[0015] S6: Weigh 0.1-0.5 parts by weight of paclobutrazol, add it to the solution prepared in step S5, stir evenly, and let it stand for 10 minutes;

[0016] S7: Use an electronic balance to accurately weigh the pepper extract, add it to the solution prepared in step S6, stir it evenly with a stirring tool, and let it stand for 10 minutes to allow the pepper extract to fully dissolve;

[0017] S8: Use an electronic balance to accurately weigh the Datura truncatula extract, add it to the solution prepared in step S7, stir it evenly with a stirring tool, and let it stand for 10 minutes to allow the Datura truncatula extract to fully dissolve;

[0018] S9: Use a measuring cylinder to measure distilled water and add it to the solution, stirring while adding until the total volume of the solution reaches 100 parts by weight. Use a stirring tool to fully stir the solution to ensure that the components are evenly distributed to obtain the final product. The final product is collected and packaged, and the entire preparation process of the eco-friendly foliar barrier agent for arsenic pollution of rice is completed.

[0019] In a preferred embodiment, in step S1, 10 grams of potassium glutamate are accurately weighed using an electronic balance with an accuracy of 0.0001 grams to ensure that the weighing is accurate; the weighed potassium glutamate is placed in a pre-cleaned 100 ml beaker, which should be made of high borosilicate glass to withstand temperature changes during subsequent heating and stirring; then, distilled water is added milliliter by milliliter using a pipette, and the distilled water must be pre-distilled twice to ensure its purity; during the addition of distilled water, a magnetic stirrer is used to continuously stir at a speed of 600 rpm, stirring while adding until the potassium glutamate is completely dissolved; during the dissolution process, it can be appropriately heated to 30-35 degrees Celsius to accelerate dissolution, but the temperature must be controlled not to exceed 40 degrees Celsius to avoid decomposition of potassium glutamate; after the dissolution is completed, it is allowed to stand for 5 minutes to observe whether there are any undissolved particles to ensure that the solution is completely clear.

[0020] In a preferred embodiment, in step S2, 5 g of S-allyl-L-cysteine ​​is accurately weighed using an electronic balance with an accuracy of 0.0001 g, and is slowly added to the potassium glutamate solution prepared in step S1; during the addition process, a magnetic stirrer is continuously stirred at a speed of 800 rpm to ensure that the S-allyl-L-cysteine ​​is evenly dispersed in the solution; after stirring, the solution is allowed to stand for 10 minutes to allow the S-allyl-L-cysteine ​​to fully dissolve; during this period, the pH value of the solution is monitored using a pH meter to ensure that it is maintained in the range of 6.5-7.5.

[0021] In a preferred embodiment, in step S3, 2 grams of o-hydroxypiperidinic acid are weighed using an electronic balance with an accuracy of 0.01 grams and slowly added to the solution prepared in step S2; during the addition process, a magnetic stirrer is used to continuously stir at a speed of 1000 rpm to ensure that the o-hydroxypiperidinic acid is evenly dispersed and dissolved; after stirring, the solution is allowed to stand for 10 minutes to observe whether precipitation or turbidity occurs; during this period, the absorbance of the solution can be measured using a UV-visible spectrophotometer to ensure that the o-hydroxypiperidinic acid is completely dissolved and the absorbance of the solution is as expected.

[0022] In a preferred embodiment, in step S4, after each component is added, a magnetic stirrer is used to continuously stir at a speed of 1200 rpm for 5 minutes to ensure that each component is evenly dispersed and dissolved; during the addition process, the temperature of the solution must be controlled not to exceed 30 degrees Celsius to avoid decomposition of certain components due to high temperature.

[0023] In a preferred embodiment, in step S5, 0.1 g of gibberellin is accurately weighed using an electronic balance with an accuracy of 0.0001 g, and the mixture is slowly added to the solution prepared in step S4; during the addition process, a magnetic stirrer is continuously stirred at a speed of 1400 rpm to ensure that the gibberellin is evenly dispersed and dissolved; after stirring, the mixture is allowed to stand for 10 minutes to allow the gibberellin to fully dissolve; during this period, the content of gibberellin in the solution can be measured using a high performance liquid chromatograph to ensure that its concentration meets the expected value;

[0024] In step S6, 0.3 g of paclobutrazol is weighed using an electronic balance with an accuracy of 0.01 g, and the mixture is slowly added to the solution prepared in step S5; during the addition process, a magnetic stirrer is continuously stirred at a speed of 1600 rpm to ensure that the paclobutrazol is evenly dispersed and dissolved; after stirring, the mixture is allowed to stand for 10 minutes to observe whether the solution shows precipitation or turbidity; during this period, the content of paclobutrazol in the solution can be measured using a gas chromatograph to ensure that its concentration meets the expected value.

[0025] In a preferred embodiment, in step S7, 1 gram of capsicum extract is accurately weighed using an electronic balance with an accuracy of 0.0001 grams and slowly added to the solution prepared in step S6; during the addition process, a magnetic stirrer is used to continuously stir at a speed of 1800 rpm to ensure that the capsicum extract is evenly dispersed and dissolved; after stirring is completed, the solution is allowed to stand for 10 minutes to allow the capsicum extract to fully dissolve; during this period, the content of capsicum extract in the solution can be determined using liquid chromatography-mass spectrometry to ensure that its concentration meets the expected level.

[0026] In a preferred embodiment, in step S8, an electronic balance with an accuracy of 0.0001 grams is used to accurately weigh 0.5 grams of datura flower extract and slowly add it to the solution prepared in step S7; during the addition process, a magnetic stirrer is used to continuously stir at a speed of 2000 rpm to ensure that the datura flower extract is evenly dispersed and dissolved; after stirring is completed, the mixture is allowed to stand for 10 minutes to allow the datura flower extract to be fully dispersed and dissolved.

[0027] In a preferred embodiment, in step S9, a measuring cylinder is used to accurately measure an appropriate amount of distilled water, which is also subject to secondary distillation to ensure its high purity; the distilled water is slowly added to the solution prepared in step S8 while continuously stirring at 2200 rpm using a magnetic stirrer.

[0028] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0029] 1. In the present invention, the combined use of potassium glutamate, S-allyl-L-cysteine, and o-hydroxypiperidinic acid has a positive impact on the growth and stress resistance of rice. The potassium element in potassium glutamate is involved in the activation of multiple enzymes, promoting photosynthesis and protein synthesis, and contributing to the growth and development of rice. S-allyl-L-cysteine ​​not only has the function of complexing arsenic ions, but also acts as an antioxidant to scavenge free radicals, alleviate the oxidative stress reaction caused by arsenic, and protect rice cells from damage. In addition to enhancing the complexing effect, o-hydroxypiperidinic acid may also improve rice's adaptability to arsenic pollution by affecting the ion balance in rice and optimizing the intracellular microenvironment. The synergistic effect of these three ingredients not only effectively reduces rice's absorption and accumulation of arsenic, but also enhances the overall health of rice by improving its growth conditions and physiological state.

[0030] 2. In the present invention, potassium glutamate, as a kind of potash fertilizer, not only provides the potassium element necessary for rice growth, but also enhances the strength of cell walls and the osmotic regulation ability of cells through the influence of potassium ions, thereby improving rice's tolerance to heavy metals such as arsenic. Potassium can also promote the development of rice roots, enhance the root system's ability to absorb nutrients, and indirectly reduce the absorption of arsenic. S-allyl-L-cysteine, as an amino acid derivative containing a thiol group, has a strong complexing ability and can form a stable complex with arsenic ions, thereby effectively preventing the transportation and accumulation of arsenic in rice. This complexing action not only reduces the toxicity of arsenic, but also reduces the damage of arsenic to rice growth points. The addition of o-hydroxypiperidinic acid further enhances the complexing effect. The hydroxyl group and piperidine ring in its molecular structure can undergo multi-point binding with arsenic ions to form a more stable complex, thereby improving the overall effect of the inhibitor.

[0031] 3. In this invention, the optimal ratio of gibberellins and paclobutrazol regulates rice growth and development. Gibberellins promote cell elongation and division, aiding rice growth, while paclobutrazol inhibits excessive growth, promoting robust rice growth. The combined use of these two regulators ensures rice growth while minimizing the negative impact of arsenic contamination. The addition of capsicum and datura extracts, as botanical ingredients, not only provides additional bioactive substances but also potentially enhances rice tolerance to arsenic through their unique alkaloid composition. Finally, the use of purified water as a solvent ensures uniformity and stability of the inhibitor, making it easy to spray and apply. Overall, the beneficial effects of this formulation are manifested in multiple aspects: first, it effectively blocks arsenic absorption by rice, reducing its accumulation; second, it enhances rice growth and stress resistance by providing nutrients and regulating growth; and third, its use of eco-friendly ingredients reduces environmental impact, meeting the requirements of sustainable agriculture. The comprehensive benefits of this inhibitor provide a strong guarantee for safe rice production. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the process of the present invention. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0034] Reference Figure 1 ,

[0035] Example:

[0036] Eco-friendly foliar control agent for arsenic contamination in rice, including:

[0037] Potassium glutamate: 5 parts by weight; S-allyl-L-cysteine: 1 part by weight; o-hydroxypipecolic acid: 1 part by weight; seaweed extract: 3 parts by weight; licorice extract: 3 parts by weight; astragalus extract: 3 parts by weight; isatis root extract: 3 parts by weight; citric acid: 1 part by weight; tea saponin: 1 part by weight; gum arabic: 1 part by weight; xanthan gum: 1 part by weight; silicate: 2 parts by weight; calcium magnesium fertilizer: 1 part by weight;

[0038] Plant growth regulator: gibberellin: 0.1 parts by weight; paclobutrazol: 0.1 parts by weight;

[0039] Plant ingredients: pepper extract: 0.5 parts by weight; datura flower extract: 0.5 parts by weight; purified water: 70 parts by weight.

[0040] The preparation method comprises the following steps:

[0041] S1: Use an electronic balance to accurately weigh potassium glutamate, place it in a clean beaker, add distilled water, and stir until the potassium glutamate is completely dissolved;

[0042] S2: Accurately weigh S-allyl-L-cysteine ​​using an electronic balance, add it to the potassium glutamate solution, stir evenly using a stirring tool, and let it stand for 10 minutes to allow the S-allyl-L-cysteine ​​to fully dissolve;

[0043] S3: Weigh 1-3 parts by weight of o-hydroxypiperidinic acid, add it to the solution prepared in step S2, stir evenly, and let it stand for 10 minutes;

[0044] S4: Add seaweed extract, licorice extract, astragalus extract, isatis root extract, citric acid, tea saponin, gum arabic, xanthan gum, silicate, and calcium magnesium fertilizer in sequence, stirring evenly after adding each ingredient;

[0045] S5: Use an electronic balance to accurately weigh gibberellin, add it to the solution prepared in step S4, stir evenly using a stirring tool, and let it stand for 10 minutes to allow the gibberellin to fully dissolve;

[0046] S6: Weigh 0.1-0.5 parts by weight of paclobutrazol, add it to the solution prepared in step S5, stir evenly, and let it stand for 10 minutes;

[0047] S7: Use an electronic balance to accurately weigh the pepper extract, add it to the solution prepared in step S6, stir it evenly with a stirring tool, and let it stand for 10 minutes to allow the pepper extract to fully dissolve;

[0048] S8: Use an electronic balance to accurately weigh the Datura truncatula extract, add it to the solution prepared in step S7, stir it evenly with a stirring tool, and let it stand for 10 minutes to allow the Datura truncatula extract to fully dissolve;

[0049] S9: Use a measuring cylinder to measure distilled water and add it to the solution, stirring while adding until the total volume of the solution reaches 100 parts by weight. Use a stirring tool to fully stir the solution to ensure that the components are evenly distributed to obtain the final product. The final product is collected and packaged, and the entire preparation process of the eco-friendly foliar barrier agent for arsenic pollution of rice is completed.

[0050] In described step S1, use the electronic balance with the precision of 0.0001 gram to accurately weigh 10 grams of potassium glutamate, ensure that weighing is accurate. The potassium glutamate weighed is put into a 100 milliliters of beakers cleaned in advance, and this beaker should adopt high borosilicate glass material, to withstand the temperature change in subsequent heating and stirring process. Then, use a pipette to add distilled water by milliliters, and distilled water needs to be double distilled in advance to ensure its purity. In the process of adding distilled water, use a magnetic stirrer to continuously stir at a speed of 600 rev / min, stirring while adding, until potassium glutamate is completely dissolved. During the dissolution process, it can be suitably heated to 30-35 degrees Celsius to accelerate dissolution, but the temperature needs to be controlled not to exceed 40 degrees Celsius to avoid the decomposition of potassium glutamate. After dissolution is completed, stand for 5 minutes to observe whether there are undissolved particles to ensure that the solution is completely clear.

[0051] In described step S2, use the electronic balance that precision is 0.0001 gram, accurately weigh 5 grams of S-allyl-L-cysteine, it is slowly added in the potassium glutamate solution that step S1 prepares.During adding process, use magnetic stirring apparatus to continue stirring with the rotating speed of 800 rev / min, ensure that S-allyl-L-cysteine ​​is evenly dispersed in solution.After stirring is completed, stand 10 minutes, allow S-allyl-L-cysteine ​​fully dissolve.During this period, pH meter can be used to monitor the pH value of solution, ensure that it maintains in the range of 6.5-7.5, if necessary, dilute hydrochloric acid or sodium hydroxide solution can be used to make fine adjustment.

[0052] In the step S3, 2 grams of o-hydroxy pipecolic acid are weighed using an electronic balance with an accuracy of 0.01 grams and slowly added to the solution prepared in step S2. During the addition process, a magnetic stirrer is used to continuously stir at a speed of 1000 rpm to ensure that o-hydroxy pipecolic acid is evenly dispersed and dissolved. After stirring is completed, the mixture is allowed to stand for 10 minutes to observe whether the solution precipitates or becomes turbid. During this period, a UV-visible spectrophotometer can be used to measure the absorbance of the solution to ensure that o-hydroxy pipecolic acid is completely dissolved and the absorbance of the solution meets expectations.

[0053] In step S4, after each component is added, the mixture is stirred continuously for 5 minutes at 1200 rpm using a magnetic stirrer to ensure that each component is evenly dispersed and dissolved. During the addition process, the temperature of the solution must be controlled to not exceed 30 degrees Celsius to prevent decomposition of certain components due to high temperature.

[0054] In described step S5, use the electronic balance that precision is 0.0001 gram, accurately weigh 0.1 gram of gibberellins, slowly add in the solution that step S4 prepares.In adding process, use magnetic stirring apparatus to continue stirring with the rotating speed of 1400 rev / min, guarantee that gibberellins are evenly dispersed and dissolve.After stirring is complete, leave standstill 10 minutes, allow gibberellins fully dissolve.During this period, can use the content of gibberellins in high performance liquid chromatograph determination solution, guarantee that its concentration meets expectation.

[0055] In described step S6, use the electronic balance that precision is 0.01 gram, weigh 0.3 gram of paclobutrazol, slowly be added in the solution that step S5 prepares.In adding process, use magnetic stirring apparatus to continue stirring with the rotating speed of 1600 rev / min, guarantee that paclobutrazol is evenly dispersed and dissolves.After stirring is complete, leave standstill 10 minutes, observe whether solution appears precipitation or turbidity.During this period, can use the content of paclobutrazol in gas chromatograph determination solution, guarantee that its concentration meets expection.

[0056] In step S7, 1 gram of chili extract is accurately weighed using an electronic balance with an accuracy of 0.0001 grams and slowly added to the solution prepared in step S6. During the addition process, a magnetic stirrer is continuously stirred at 1800 rpm to ensure that the chili extract is evenly dispersed and dissolved. After stirring, the solution is allowed to stand for 10 minutes to allow the chili extract to fully dissolve. During this time, the content of the chili extract in the solution can be measured using liquid chromatography-mass spectrometry to ensure that its concentration meets the desired level.

[0057] In step S8, 0.5 g of datura flower extract was accurately weighed using an electronic balance with an accuracy of 0.0001 g and slowly added to the solution prepared in step S7. During the addition process, a magnetic stirrer was used to continuously stir at a speed of 2000 rpm to ensure that the datura flower extract was evenly dispersed and dissolved. After stirring, the mixture was allowed to stand for 10 minutes to allow the datura flower extract to be fully dispersed and dissolved.

[0058] In the step S9, a graduated cylinder is used to accurately measure an appropriate amount of distilled water, which also needs to be secondary distilled to ensure its high purity. Distilled water is slowly added to the solution prepared in step S8, while adding a magnetic stirrer while continuously stirring at a speed of 2200 revs / min to ensure the uniformity of the solution. The purpose of adding distilled water is to adjust the cumulative volume of the solution to 100 milliliters, so the volume of the solution needs to be continuously monitored during the addition process to avoid excessive addition.

[0059] As the total volume approaches 100 mL, slow the addition of distilled water and use a pipette to precisely adjust the volume until it reaches the 100 mL mark. Continue stirring the solution with a magnetic stirrer for 10 minutes to ensure that all components are thoroughly mixed and evenly distributed.

[0060] During the stirring process, an ultrasonic cleaner can be used to ultrasonically treat the solution to further eliminate tiny bubbles and agglomerates in the solution and improve the stability and uniformity of the solution. The power of the ultrasonic treatment is set to 100 watts and the treatment time is 5 minutes.

[0061] After stirring and ultrasonic treatment, let the solution stand for 5 minutes and observe its appearance to ensure clarity and absence of precipitation. Once the solution is of acceptable quality, collect and package the final product in pre-sterilized glass or plastic bottles. During packaging, avoid contact with air to prevent oxidation or contamination of the solution.

[0062] After packaging is completed, affix the product label and indicate the product name, preparation date, batch number and other information for subsequent use and traceability. At this point, the entire preparation process of the eco-friendly foliar barrier for rice arsenic pollution is completed. The product can be stored in a cool, dry, dark environment to prepare a measuring cylinder to accurately measure an appropriate amount of distilled water. The distilled water also needs to be distilled twice to ensure its high purity. Slowly add distilled water to the solution prepared in step S8, while adding it, use a magnetic stirrer to continuously stir at a speed of 2200 rpm to ensure the uniformity of the solution. The purpose of adding distilled water is to adjust the total volume of the solution to 100 ml, so the volume of the solution needs to be continuously monitored during the addition process to avoid excessive amounts.

[0063] As the total volume approaches 100 mL, slow the addition of distilled water and use a pipette to precisely adjust the volume until it reaches the 100 mL mark. Continue stirring the solution with a magnetic stirrer for 10 minutes to ensure that all components are thoroughly mixed and evenly distributed.

[0064] During the stirring process, an ultrasonic cleaner can be used to ultrasonically treat the solution to further eliminate tiny bubbles and agglomerates in the solution and improve the stability and uniformity of the solution. The power of the ultrasonic treatment is set to 100 watts and the treatment time is 5 minutes.

[0065] After stirring and ultrasonic treatment, let the solution stand for 5 minutes and observe its appearance to ensure clarity and absence of precipitation. Once the solution is of acceptable quality, collect and package the final product in pre-sterilized glass or plastic bottles. During packaging, avoid contact with air to prevent oxidation or contamination of the solution.

[0066] After packaging, label the product with the product name, preparation date, batch number, and other information for easy traceability. This completes the preparation process for the eco-friendly foliar barrier for arsenic contamination in rice. The product can be stored in a cool, dry, dark environment until ready for use.

[0067] In the present invention, the combined use of potassium glutamate, S-allyl-L-cysteine ​​and o-hydroxypiperidinic acid has a positive impact on the growth and stress resistance of rice. The potassium element in potassium glutamate is involved in the activation of multiple enzymes, promoting photosynthesis and protein synthesis, and contributing to the growth and development of rice. S-allyl-L-cysteine ​​not only has the function of complexing arsenic ions, but also can act as an antioxidant to scavenge free radicals, reduce the oxidative stress reaction caused by arsenic, and protect rice cells from damage. In addition to enhancing the complexing effect, o-hydroxypiperidinic acid may also improve the adaptability of rice to arsenic pollution by affecting the ion balance in the rice body and optimizing the microenvironment within the cell. The synergistic effect of these three not only effectively reduces the absorption and accumulation of arsenic in rice, but also enhances the overall health of rice by improving the growth conditions and physiological state of rice.

[0068] In the present invention, potassium glutamate, as a kind of potash fertilizer, not only provides the potassium element necessary for rice growth, but also enhances the strength of cell walls and the osmotic regulation ability of cells through the influence of potassium ions, thereby improving rice's tolerance to heavy metals such as arsenic. Potassium can also promote the development of rice roots, enhance the root system's ability to absorb nutrients, and indirectly reduce the absorption of arsenic. S-allyl-L-cysteine, as an amino acid derivative containing a thiol group, has a strong complexing ability and can form a stable complex with arsenic ions, thereby effectively preventing the transportation and accumulation of arsenic in rice. This complexing action not only reduces the toxicity of arsenic, but also reduces the damage of arsenic to rice growth points. The addition of o-hydroxypiperidinic acid further enhances the complexing effect. The hydroxyl group and piperidine ring in its molecular structure can undergo multi-point binding with arsenic ions to form a more stable complex, thereby improving the overall effect of the inhibitor.

[0069] In this invention, the rational ratio of gibberellins and paclobutrazol regulates rice growth and development. Gibberellins promote cell elongation and division, aiding rice growth, while paclobutrazol inhibits excessive growth, promoting robust rice growth. The combined use of these two regulators ensures rice growth while minimizing the negative impact of arsenic contamination on rice growth. The addition of capsicum and datura extracts, as botanical ingredients, not only provides additional bioactive substances but also potentially enhances rice tolerance to arsenic through their unique alkaloid composition. Finally, the use of purified water as a solvent ensures uniformity and stability of the inhibitor, making it easy to spray and apply. Overall, the beneficial effects of this formulation are manifested in multiple aspects: first, it effectively blocks arsenic absorption by rice, reducing its accumulation in the plant; second, it enhances rice growth and stress resistance by providing nutrition and regulating growth; and third, its use of eco-friendly ingredients reduces environmental impact, meeting the requirements of sustainable agriculture. The comprehensive benefits of this inhibitor provide a strong guarantee for safe rice production.

[0070] Case Study: Evaluation of the Effectiveness of Eco-Friendly Foliar Control Agents for Arsenic Contamination in Rice

[0071] 1. Purpose of the Test

[0072] The effects of the eco-friendly rice arsenic pollution foliar control agent of the present invention on reducing arsenic absorption by rice and enhancing rice growth and stress resistance were evaluated.

[0073] 2. Test Materials

[0074] Rice variety: a conventional rice variety.

[0075] Foliar control agent: an eco-friendly foliar control agent for arsenic pollution of rice prepared according to the method of the present invention.

[0076] Control group: conventional foliar fertilizer.

[0077] 3. Experimental Design

[0078] The experiment was divided into two groups: Example and control group.

[0079] Each group had three replicates, and 100 rice plants were planted in each replicate.

[0080] Example The foliar control agent was sprayed once during each of the key growth stages of rice (tillering stage, jointing stage, and booting stage), and the control group was sprayed with an equal amount of conventional foliar fertilizer during the same period.

[0081] 4. Test methods

[0082] Rice cultivation: Planting rice on arsenic-contaminated soil ensures consistent soil arsenic levels.

[0083] Foliar spraying: Foliar spraying was carried out according to the experimental design.

[0084] Sample collection: During the rice maturity period, rice plant and rice samples were collected.

[0085] Index determination: Determination of arsenic content in rice plants and rice grains, rice growth indicators (such as plant height, biomass, root development, etc.) and stress resistance indicators (such as antioxidant enzyme activity, etc.).

[0086] 5. Test data are shown in the table below:

[0087] Table 1 Arsenic content in rice plants and rice (unit: mg / kg)

[0088] Group Arsenic content in rice plants Arsenic content in rice Treatment group 5.2±0.3 0.8±0.1 control group 12.5±0.8 2.3±0.2

[0089] Table 2 Rice growth indicators

[0090] Group Plant height (cm) Biomass (g / plant) Root development index Treatment group 110±5 35±2 1.8±0.1 control group 95±4 28±1.5 1.2±0.1

[0091] Table 3 Rice stress resistance index

[0092] Group Antioxidant enzyme activity (U / g) Treatment group 120±10 control group 80±5

[0093] From the above we can know:

[0094] In the present invention, the combined use of potassium glutamate, S-allyl-L-cysteine ​​and o-hydroxypiperidinic acid has a positive impact on the growth and stress resistance of rice. The potassium element in potassium glutamate is involved in the activation of multiple enzymes, promoting photosynthesis and protein synthesis, and contributing to the growth and development of rice. S-allyl-L-cysteine ​​not only has the function of complexing arsenic ions, but also can act as an antioxidant to scavenge free radicals, reduce the oxidative stress reaction caused by arsenic, and protect rice cells from damage. In addition to enhancing the complexing effect, o-hydroxypiperidinic acid may also improve the adaptability of rice to arsenic pollution by affecting the ion balance in the rice body and optimizing the microenvironment within the cell. The synergistic effect of these three not only effectively reduces the absorption and accumulation of arsenic in rice, but also enhances the overall health of rice by improving the growth conditions and physiological state of rice.

[0095] In the present invention, potassium glutamate, as a kind of potash fertilizer, not only provides the potassium element necessary for rice growth, but also enhances the strength of cell walls and the osmotic regulation ability of cells through the influence of potassium ions, thereby improving rice's tolerance to heavy metals such as arsenic. Potassium can also promote the development of rice roots, enhance the root system's ability to absorb nutrients, and indirectly reduce the absorption of arsenic. S-allyl-L-cysteine, as an amino acid derivative containing a thiol group, has a strong complexing ability and can form a stable complex with arsenic ions, thereby effectively preventing the transportation and accumulation of arsenic in rice. This complexing action not only reduces the toxicity of arsenic, but also reduces the damage of arsenic to rice growth points. The addition of o-hydroxypiperidinic acid further enhances the complexing effect. The hydroxyl group and piperidine ring in its molecular structure can undergo multi-point binding with arsenic ions to form a more stable complex, thereby improving the overall effect of the inhibitor.

[0096] In this invention, the rational ratio of gibberellins and paclobutrazol regulates rice growth and development. Gibberellins promote cell elongation and division, aiding rice growth, while paclobutrazol inhibits excessive growth, promoting robust rice growth. The combined use of these two regulators ensures rice growth while minimizing the negative impact of arsenic contamination on rice growth. The addition of capsicum and datura extracts, as botanical ingredients, not only provides additional bioactive substances but also potentially enhances rice tolerance to arsenic through their unique alkaloid composition. Finally, the use of purified water as a solvent ensures uniformity and stability of the inhibitor, making it easy to spray and apply. Overall, the beneficial effects of this formulation are manifested in multiple aspects: first, it effectively blocks arsenic absorption by rice, reducing its accumulation in the plant; second, it enhances rice growth and stress resistance by providing nutrition and regulating growth; and third, its use of eco-friendly ingredients reduces environmental impact, meeting the requirements of sustainable agriculture. The comprehensive benefits of this inhibitor provide a strong guarantee for safe rice production.

[0097] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0098] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. An eco-friendly foliar control agent for arsenic contamination of rice, characterized by: include: Potassium glutamate: 5-10 parts by weight; S-allyl-L-cysteine: 1-3 parts by weight; o-hydroxypiperidinic acid: 1-3 parts by weight; seaweed extract: 3-7 parts by weight; licorice extract: 3-7 parts by weight; astragalus extract: 3-7 parts by weight; isatis root extract: 3-7 parts by weight; Citric acid: 1-5 parts by weight; Tea saponin: 1-5 parts by weight; Gum arabic: 1-5 parts by weight; Xanthan gum: 1-5 parts by weight; Silicate: 2-5 parts by weight; Calcium magnesium fertilizer: 1-3 parts by weight; Plant growth regulators: Gibberellic acid: 0.1-0.5 parts by weight; Paclobutrazol: 0.1-0.5 parts by weight; Plant ingredients: pepper extract: 0.5-1 parts by weight; datura flower extract: 0.5-1 parts by weight; purified water: 70-80 parts by weight.

2. The method for preparing the eco-friendly foliar barrier agent for arsenic contamination of rice according to claim 1, characterized in that: The preparation method comprises the following steps: S1: Use an electronic balance to accurately weigh potassium glutamate, place it in a clean beaker, add distilled water, and stir until the potassium glutamate is completely dissolved; S2: Accurately weigh S-allyl-L-cysteine ​​using an electronic balance, add it to the potassium glutamate solution, stir evenly using a stirring tool, and let it stand for 10 minutes to allow the S-allyl-L-cysteine ​​to fully dissolve; S3: Weigh 1-3 parts by weight of o-hydroxypiperidinic acid, add it to the solution prepared in step S2, stir evenly, and let it stand for 10 minutes; S4: Add seaweed extract, licorice extract, astragalus extract, isatis root extract, citric acid, tea saponin, gum arabic, xanthan gum, silicate, and calcium magnesium fertilizer in sequence, stirring evenly after adding each ingredient; S5: Use an electronic balance to accurately weigh gibberellin, add it to the solution prepared in step S4, stir evenly using a stirring tool, and let it stand for 10 minutes to allow the gibberellin to fully dissolve; S6: Weigh 0.1-0.5 parts by weight of paclobutrazol, add it to the solution prepared in step S5, stir evenly, and let it stand for 10 minutes; S7: Use an electronic balance to accurately weigh the pepper extract, add it to the solution prepared in step S6, stir it evenly with a stirring tool, and let it stand for 10 minutes to allow the pepper extract to fully dissolve; S8: Use an electronic balance to accurately weigh the Datura truncatula extract, add it to the solution prepared in step S7, stir it evenly with a stirring tool, and let it stand for 10 minutes to allow the Datura truncatula extract to fully dissolve; S9: Use a measuring cylinder to measure distilled water and add it to the solution, stirring while adding until the total volume of the solution reaches 100 parts by weight. Use a stirring tool to fully stir the solution to ensure that the components are evenly distributed to obtain the final product. The final product is collected and packaged, and the entire preparation process of the eco-friendly foliar barrier agent for arsenic pollution of rice is completed.

3. The method for preparing the eco-friendly foliar barrier agent for arsenic contamination of rice according to claim 1, characterized in that: In step S1, 10 grams of potassium glutamate are accurately weighed using an electronic balance with an accuracy of 0.0001 grams to ensure that the weighing is accurate; the weighed potassium glutamate is placed in a pre-cleaned 100-ml beaker, which should be made of high borosilicate glass to withstand temperature changes during subsequent heating and stirring; then, distilled water is added milliliter by milliliter using a pipette, and the distilled water must be pre-distilled twice to ensure its purity; During the process of adding distilled water, use a magnetic stirrer to stir continuously at a speed of 600 rpm, stirring while adding until the potassium glutamate is completely dissolved; during the dissolution process, it can be heated to 30-35 degrees Celsius to accelerate the dissolution, but the temperature must be controlled not to exceed 40 degrees Celsius to avoid decomposition of potassium glutamate; after the dissolution is completed, let it stand for 5 minutes and observe whether there are any undissolved particles to ensure that the solution is completely clear.

4. The method for preparing the eco-friendly foliar barrier agent for arsenic contamination of rice according to claim 1, characterized in that: In step S2, 5 g of S-allyl-L-cysteine ​​is accurately weighed using an electronic balance with an accuracy of 0.0001 g, and the mixture is slowly added to the potassium glutamate solution prepared in step S1; during the addition process, a magnetic stirrer is continuously stirred at a speed of 800 rpm to ensure that the S-allyl-L-cysteine ​​is evenly dispersed in the solution; after stirring, the mixture is allowed to stand for 10 minutes to allow the S-allyl-L-cysteine ​​to fully dissolve; during this period, the pH value of the solution is monitored using a pH meter to ensure that it is maintained within the range of 6.5-7.

5.

5. The method for preparing the eco-friendly foliar barrier agent for arsenic contamination of rice according to claim 1, characterized in that: In step S3, 2 g of o-hydroxypiperidinic acid was weighed using an electronic balance with an accuracy of 0.01 g, and the mixture was slowly added to the solution prepared in step S2; during the addition process, a magnetic stirrer was continuously stirred at a speed of 1000 rpm to ensure that the o-hydroxypiperidinic acid was evenly dispersed and dissolved; After stirring, let it stand for 10 minutes and observe whether the solution shows any precipitation or turbidity. During this period, the absorbance of the solution can be measured using a UV-visible spectrophotometer to ensure that the o-hydroxypiperidinic acid is completely dissolved and the absorbance of the solution is as expected.

6. The method for preparing the eco-friendly foliar barrier agent for arsenic contamination of rice according to claim 1, characterized in that: In step S4, after each component is added, a magnetic stirrer is used to continuously stir at a speed of 1200 rpm for 5 minutes to ensure that each component is evenly dispersed and dissolved; during the addition process, the temperature of the solution must be controlled not to exceed 30 degrees Celsius to avoid decomposition of certain components due to high temperature.

7. The method for preparing the eco-friendly foliar barrier for arsenic contamination of rice according to claim 1, characterized in that: In step S5, 0.1 g of gibberellin is accurately weighed using an electronic balance with an accuracy of 0.0001 g, and the mixture is slowly added to the solution prepared in step S4; during the addition process, a magnetic stirrer is continuously stirred at a speed of 1400 rpm to ensure that the gibberellin is evenly dispersed and dissolved; after stirring, the mixture is allowed to stand for 10 minutes to allow the gibberellin to fully dissolve; during this period, the content of the gibberellin in the solution can be measured using a high performance liquid chromatograph to ensure that its concentration meets the expected value; In step S6, 0.3 g of paclobutrazol was weighed using an electronic balance with an accuracy of 0.01 g and slowly added to the solution prepared in step S5; during the addition process, a magnetic stirrer was continuously stirred at a speed of 1600 rpm to ensure that the paclobutrazol was evenly dispersed and dissolved; After stirring, let it stand for 10 minutes and observe whether the solution shows any precipitation or turbidity. During this period, a gas chromatograph can be used to measure the content of paclobutrazol in the solution to ensure that its concentration meets expectations.

8. The method for preparing the eco-friendly foliar barrier agent for arsenic contamination of rice according to claim 1, characterized in that: In step S7, 1 gram of the chili extract is accurately weighed using an electronic balance with an accuracy of 0.0001 grams, and the mixture is slowly added to the solution prepared in step S6; during the addition process, a magnetic stirrer is continuously stirred at a speed of 1800 rpm to ensure that the chili extract is evenly dispersed and dissolved; After stirring, let it stand for 10 minutes to allow the chili extract to fully dissolve; during this period, liquid chromatography-mass spectrometry can be used to determine the content of chili extract in the solution to ensure that its concentration meets expectations.

9. The method for preparing the eco-friendly foliar barrier for arsenic contamination of rice according to claim 1, characterized in that: In step S8, an electronic balance with an accuracy of 0.0001 grams is used to accurately weigh 0.5 grams of the Datura flower extract, and the mixture is slowly added to the solution prepared in step S7. During the addition process, a magnetic stirrer is used to continuously stir at a speed of 2000 rpm to ensure that the Datura flower extract is evenly dispersed and dissolved. After stirring, the mixture is allowed to stand for 10 minutes to allow the Datura flower extract to be fully dispersed and dissolved.

10. The method for preparing the eco-friendly foliar barrier agent for arsenic contamination of rice according to claim 1, characterized in that: In step S9, a measuring cylinder is used to accurately measure an appropriate amount of distilled water, which also needs to be distilled twice to ensure its high purity; Distilled water was slowly added to the solution prepared in step S8 while continuously stirring at 2200 rpm using a magnetic stirrer.

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