Halophilic microorganism and application thereof in retarding chlorine corrosion in open circulating cooling system

Abstract

The invention relates to a halophilic microorganism Vreelandella sulfidaereis FL016 and an application of the halophilic microorganism Vreelandella sulfidaereis FL016 in retarding of chlorine corrosion in an open type circulating cooling system. The halophilic microorganism Vreeldella sulfidaereis FL016 is preserved in the China Center for Type Culture Collection, and the preservation number of the halophilic microorganism Vreeldella sulfidaereis FL016 is CCTCC (China Center for Type Culture Collection) NO: 2025580. The scale inhibitor can slow down chlorine corrosion in the circulating cooling equipment, can synergistically achieve the effects of chlorine removal and scale inhibition after being compounded with the antarcinia denitrificans FL10, and has a better application prospect in the aspects of chlorine removal and / or scale inhibition and the like of an industrial circulating cooling system.

Description

Technical Field

[0001] The invention belongs to the technical field of industrial microorganisms, and particularly relates to a halophilic microorganism and an application thereof in alleviating chlorine corrosion in an open-circulation cooling system. Background Art

[0002] The industrial circulating cooling system uses the evaporation process of water to absorb heat, reduce the temperature of the process medium, ensure the normal operation of the equipment, and realize the smooth progress of industrial production. Therefore, improving the utilization rate of industrial circulating cooling water is of great practical significance for saving water resources and reducing production costs.

[0003] The cooling water concentration ratio is often used to evaluate the water-saving performance of the circulating cooling system. Therefore, it is very important to improve the cooling water concentration ratio. Industrial cooling water is generally discharged after being concentrated to 3-5 times. During the cooling system circulation process, the supplementary water added to the cooling system due to the evaporation and concentration of the cooling water is the Cl in the system. - The only source of Cl - With the characteristic of single source, Cl - The concentration is used as the basis for judging the concentration multiple of cooling water. - The concentration accumulates as the concentration factor increases. Cl- ions have strong penetrability and easily penetrate the oxide film or passivation film on the metal surface, forming small holes or pits on the metal surface, thereby corroding the system equipment. From the perspectives of safety, economy, and environmental protection, controlling chlorine corrosion and removing scale are key links. Currently, there is little research on the problem of chlorine corrosion in industrial circulating cooling systems. Therefore, the present invention aims to screen halophilic microorganisms that can absorb and metabolize chlorine-containing substances in the metabolic environment, thereby slowing down the chlorine corrosion of circulating cooling equipment and increasing the concentration factor of circulating cooling water to 10 times. Summary of the Invention

[0004] The present invention discloses a halophilic microorganism, Vreelandella sulfidaeris FL016, and its use in mitigating chlorine corrosion in open-circulation cooling systems. Vreelandella sulfidaeris FL016, deposited with the China Center for Type Culture Collection under the CCTCC No. 2025580, can mitigate chlorine corrosion in industrial circulating cooling equipment. When combined with denitrifying Zybelella denitrificans FL10, it can synergistically achieve chlorine removal and scale inhibition, demonstrating promising application prospects in chlorine removal and / or scale inhibition in industrial circulating cooling systems.

[0005] The present invention provides a halophilic microorganism Vreelandella sulfidaeris FL016, which was deposited in the China Center for Type Culture Collection, Wuhan, China on March 24, 2025, and the deposit number of the strain is CCTCC NO: 2025580.

[0006] The present invention also provides a bacterial agent, a composition and / or a water treatment reagent containing the halophilic microorganism FL016.

[0007] Furthermore, the bacterial agent, composition and / or water treatment reagent also includes denitrifying Zybelella FL10, whose deposit number is CCTCC NO: M 2024795.

[0008] The present invention also provides the use of the halophilic microorganism FL016 or the bacterial agent, composition and / or water treatment reagent in water treatment.

[0009] Furthermore, the water treatment includes dechlorination and / or scale inhibition.

[0010] Furthermore, the dechlorination is to slow down chlorine corrosion in an open-cycle cooling system.

[0011] The present invention also provides a method for removing chlorine and / or preventing scale in water, wherein the halophilic microorganism FL016 or the bacterial agent, composition and / or water treatment reagent is added into the water to be treated.

[0012] Furthermore, the water body is circulating cooling water in an open-loop cooling system.

[0013] Compared with the prior art, the present invention has the following advantages: The present invention provides a halophilic microorganism, Vreelandella sulfidaeris FL016, and its use in mitigating chlorine corrosion in open-circulation cooling systems. The halophilic microorganism, Vreelandella sulfidaeris FL016, was deposited with the China Center for Type Culture Collection, Wuhan, China, on March 24, 2025, with a deposit number of CCTCC NO: 2025580. It can mitigate chlorine corrosion in industrial circulating cooling equipment and, when combined with denitrifying Zybelella denitrificans FL10, can synergistically achieve chlorine removal and scale inhibition, showing promising application prospects in chlorine removal and / or scale inhibition in industrial circulating cooling systems. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0015] Figure 1 This is the evolutionary tree analysis diagram of the halophilic microorganism Vreelandella sulfidaeris FL016.

[0016] Figure 2 Verification of chlorine removal performance of FL016.

[0017] Figure 3 The results show the application effect of microbial agents in circulating cooling devices.

[0018] Figure 4 To verify the anti-corrosion effect of microbial agents in circulating cooling devices. DETAILED DESCRIPTION

[0019] The following examples are only used to more clearly illustrate the technical scheme of the present invention, and are therefore only used as examples, and cannot limit the scope of protection of the present invention with this. It should be noted that, unless otherwise stated, the technical terms or scientific terms used in this application should be the usual meanings understood by those skilled in the art to which the present invention belongs. Unless otherwise stated, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. Unless otherwise stated, the reagents and materials used in the following examples are commercially available.

[0020] Example 1

[0021] Screening and identification of halophilic microorganisms:

[0022] Halophilic microorganisms were isolated from the water of Chaka Salt Lake in Qinghai Province.

[0023] The microbial culture medium used for screening is as follows:

[0024] LB liquid medium (g / L): peptone 10.00, yeast powder 5.00, sodium chloride 10.00, pH 7.5

[0025] LB solid medium (g / L): peptone 10.00, yeast powder 5.00, sodium chloride 10.00, agarose 15.00, pH 7.5

[0026] LB liquid medium containing a certain concentration of sodium chloride (g / L): peptone 10.00, yeast powder 5.00, 3% sodium chloride 30.00, pH 7.5

[0027] The screening process and specific steps are as follows:

[0028] ① First screening: Sediment from the saturated sodium chloride area of ​​Chaka Salt Lake in Qinghai was inoculated into LB enrichment medium at a volume ratio of 10% in a clean bench, and a blank control was set up. The culture was shaken at 28°C and 120 rpm for 48 hours.

[0029] ② Second screening: Pipette 1% by volume of the enriched bacterial solution into LB medium containing 2% NaCl and continue enrichment culture. Ferment at 28°C, 120 rpm, and shake for 48 h. Set up a blank control.

[0030] ③Continuous screening: Pipette 1% by volume of the enriched bacterial solution into LB medium containing 3% NaCl in a concentration gradient for further enrichment and culture. Ferment at 28°C, 120 rpm, and shake for 48 hours. A blank control should be provided. Observe the growth of the microorganisms regularly. During the enrichment and screening period in 3% NaCl medium, prepare and sterilize the solid culture medium at least 24 hours in advance.

[0031] ④ Take the last culture solution and use the "cross-streaking method" to separate and culture on the plate. Culture at a constant temperature of 28℃ for 5-8 days and observe the growth of the colonies.

[0032] ⑤ After a single colony grows on the plate, pick a single colony and culture it in LB liquid medium at 28°C and 120 rpm for 48-72 h. Take 10 mL of bacterial solution from each parallel group for DNA extraction and sequencing. Centrifuge 40 mL of bacterial solution at 5000 rpm for 25 min. Pour off the supernatant in a clean bench, add 0.9 mL of sterile water and shake well. Then add 0.9 mL of sterilized 25% glycerol, shake well, transfer to a glycerol tube with a pipette, and store at -80°C.

[0033] The microorganisms obtained by the above screening were sequenced, and the 16S rRNA sequencing results were as follows:

[0034]

[0035] The phylogenetic tree was constructed based on the 16S rRNA sequencing results. Figure 1 As shown, the strain was identified and screened as the halophilic microorganism Vreelandella sulfidaeris, named FL016, and deposited in the China Center for Type Culture Collection, Wuhan, Hubei, China on March 24, 2025, with the deposit number CCTCC M2025580.

[0036] Example 2

[0037] 1. Verify the chlorine removal performance of FL016

[0038] 1‰ seed liquid was taken from the glycerol tube of the preserved strain for activation. After 48 hours, FL016 bacterial liquid was added at a concentration of 1mL / L. Three experimental groups with different sodium chloride concentrations of 1.65g / L, 0.825g / L, and 0.33g / L (chloride ion concentrations of 1g / L, 0.5g / L, and 0.2g / L) were set up to measure the changes in chloride ion concentrations from 0 to 96 hours to verify the performance of microorganism FL016 in absorbing chloride ions. The results are as follows Figure 2 As shown in the figure, the results show that the chloride ion concentration of each experimental group decreased significantly with the extension of time. FL016 can effectively absorb chloride ions in the environment. At higher concentrations, its absorption effect is more significant. It can be used in circulating cooling systems to effectively remove chlorine.

[0039] 2. Microbial agents are used in circulating cooling systems

[0040] A microbial agent composed of FL016 and denitrifying Zabelella FL010 (deposit number CCTCC NO: M 2024795) was constructed and applied to the circulating cooling system. 1‰ seed liquid was taken from the glycerol tube of the preserved strain for activation. After 48 hours, both functional microbial liquids were added at a concentration of 1mL / L. The effluent from the secondary sedimentation tank of the municipal sewage treatment plant after filtration was selected as the circulating cooling water. Scale dissolution and scale inhibition simulation circulating cooling experiments and actual application experiments of small-scale circulating cooling devices were carried out. The water quality of the effluent from the secondary sedimentation tank is shown in the following table (the calcium hardness in the table below refers to the Ca content in the water). 2+ Concentration, in CaCO3):

[0041] Table 1

[0042]

[0043] Scale dissolution experiment: Boil and concentrate the effluent from the secondary sedimentation tank to 10 times the concentration, place it in a constant temperature shaker at 28°C for 72 hours, and add microbial agents to the experimental group; scale inhibition experiment: Add microbial agents to the experimental group in a constant temperature water bath at 28°C, continue aeration evaporation and concentration, and replenish the experimental water in time to maintain the same liquid level until the concentration reaches 10 times. The results are shown in the following table:

[0044] Table 2

[0045]

[0046]

[0047] It is not difficult to see that in the scale dissolution and scale inhibition experiments, FL016 can play a good role in removing chlorine, that is, the Cl - The concentration was significantly lower than that of the blank control, and the microbial agent exhibited excellent scale inhibition performance under the acid production of denitrifying Z. denitrificans. The addition of FL010 had no significant effect on the chlorine removal efficiency of FL016. Therefore, the microbial agent composed of FL016 and Z. denitrificans has a scale inhibition and chlorine removal effect.

[0048] Application experiment of small-scale circulating cooling device: set the hot water tank temperature to 70℃, add 14L of water from the secondary sedimentation tank to the water collection tank, adjust the temperature balance to 25-30℃, absorb heat and evaporate the cooling water, replenish the experimental water in time and keep it at the 14L scale line, and hang three A3 carbon steel coupons and three 316 stainless steel coupons, and completely immerse them in the water. Measure Cl at 5 times and 10 times concentration. - concentration, coupon corrosion rate, Ca 2+ concentration and alkalinity, the results are as follows Figure 3 and Figure 4 As shown. The Industrial Circulating Cooling Water Design Specification (GB / T50050-2017) stipulates that the corrosion rate of A3 carbon steel equipment should be less than 0.075mm / a, and the corrosion rate of 316 stainless steel equipment should be less than 0.005mm / a. The results showed that the microbial agent group performed significantly better than the control group. Not only did it effectively inhibit scale and remove chlorine, but FL016 also demonstrated excellent corrosion resistance in circulating cooling systems using the effluent from the secondary sedimentation tank of a municipal sewage treatment plant as cooling water. When the circulating cooling water was concentrated to 10 times, the corrosion rates of the A3 carbon steel and 316 stainless steel coupons were lower than the national standard, meeting national standards. This indicates that the microbial agent can be used for the treatment of industrial circulating cooling water and has broad application prospects.

[0049] In summary, the FL016 screened in the present invention has good performance in chlorine removal and corrosion prevention in the circulating cooling system, and can work synergistically with scale-inhibiting microorganisms to concentrate the circulating cooling water up to 10 times by slowing down chlorine corrosion and inhibiting calcium scaling.

[0050] The above specific embodiments describe the implementation of the present invention in detail, but the present invention is not limited to the specific details of the above embodiments. Within the scope of the claims and technical concept of the present invention, various simple modifications and changes can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

Claims

1. A halophilic microorganism Vreelandella sulfidaeris FL016, characterized in that: It is deposited in China Center for Type Culture Collection with the deposit number CCTCC NO: 2025580.

2. A bacterial agent, composition and / or water treatment reagent containing the halophilic microorganism FL016 according to claim 1.

3. The bacterial agent, composition and / or water treatment reagent according to claim 2, characterized in that: Also included is Zabelella denitrificans FL10.

4. Use of the halophilic microorganism FL016 according to claim 1 or the bacterial agent, composition and / or water treatment reagent according to any one of claims 2 to 3 in water treatment.

5. The use according to claim 4, characterized in that The water treatment includes dechlorination and / or scale inhibition.

6. The use according to claim 4, characterized in that The chlorine removal is to slow down the chlorine corrosion in the open cycle cooling system.

7. A method for removing chlorine and / or preventing scale in water, characterized in that: The halophilic microorganism FL016 according to claim 1 or the bacterial agent, composition and / or water treatment reagent according to any one of claims 2-3 is added into the water body to be treated.

8. The method according to claim 7, characterized in that The water body is circulating cooling water in an open-cycle cooling system.

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