Gardenia jasminoides and fennel composite extract, preparation method and application thereof

Abstract

The invention discloses a gardenia and fennel composite extract, a preparation method and application thereof, and belongs to the technical field of plant extracts. The preparation method of the gardenia and fennel composite extract comprises: S1: preparing a gardenia extract; S2: preparing a fennel extract; S3: mixing the fennel extract and the gardenia extract according to a certain ratio to obtain the gardenia and fennel composite extract. The fennel extract prepared by the above preparation method comprises an oil component and a water-soluble component. The composite extract achieves the effect of 1+1>2, has significant effects of lowering blood lipids and blood sugar, has no side effects, has a significant regulating effect on patients' blood pressure and blood lipids, has the efficacy of improving patients' blood pressure, blood lipids and blood sugar, is non-toxic and harmless to the human body, and has no side effects.

Description

Technical Field

[0001] The present invention relates to the technical field of plant extracts, in particular to a gardenia and fennel composite extract, and a preparation method and application thereof. Background Art

[0002] Currently, the drugs used to treat diabetes and cardiovascular diseases are mainly chemical drugs, such as sulfonylureas, biguanides, etc., but these drugs are prone to cause large side effects to the human body, which in turn affects human health.

[0003] In recent years, scientists have studied Chinese herbal compositions for lowering blood sugar or blood lipids. For example, CN106074971B discloses a Chinese herbal composition for lowering blood sugar, which mainly includes a variety of natural substances such as mulberry leaves, bitter melon, and kudzu root, and can thereby play a role in lowering blood sugar without producing side effects. However, the Chinese herbal composition cannot play a role in lowering blood lipids. For example, CN106728155A discloses a Mongolian medicine composition for lowering blood lipids and resisting atherosclerosis. The raw materials of the composition include gardenia extract, bezoar and other substances. The composition can lower blood lipids without adverse reactions, and the preparation method is simple. Although it includes gardenia extract, the composition cannot play a synergistic role in lowering blood lipids and blood sugar.

[0004] Modern society has seen significant changes in lifestyles and dietary habits, leading to an increase in the incidence of chronic diseases, particularly those associated with abnormal blood lipid and blood sugar levels, such as cardiovascular disease and diabetes. Faced with these health challenges, people are increasingly seeking to maintain or improve their health. Therefore, there is a need to develop compounds that can improve patients' blood pressure, blood lipids, and blood sugar levels without toxicity or side effects. Summary of the Invention

[0005] The purpose of the present invention is to provide a composite extract of gardenia and fennel and its preparation method and application, so as to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A method for preparing a composite extract of gardenia and fennel comprises the following steps:

[0008] S1: Preparation of Gardenia Extract: Grind and sieve Gardenia into coarse powder, add water and extract several times to obtain extract, combine the extracts to obtain a first crude extract; add composite bacteria to the first crude extract, the composite bacteria including Lactobacillus acidophilus, Bifidobacterium lactis and Lactobacillus bulgaricus, the amount of Lactobacillus acidophilus is 10 6 -10 7 CFU / mL first crude extract; the dosage of Bifidobacterium lactis is 10 7 -108 CFU / mL first crude extract; the dosage of Lactobacillus bulgaricus is 10 8 -10 9 CFU / mL first crude extract;

[0009] Fermenting at 38-40°C for 7-9 hours, sterilizing to obtain a first fermentation liquid, adding ethanol to the first fermentation liquid for extraction to obtain an ethanol extract, allowing to stand, filtering and concentrating until there is no alcohol taste, adding water, refrigerating, and filtering, concentrating the filtrate to obtain a thick paste, and drying and crushing to obtain a gardenia extract;

[0010] S2: Preparation of fennel extract: Fennel powder and water are mixed to obtain a soaking liquid, wherein the weight ratio of fennel powder to water is 1:7-9, and animal Bifidobacterium is added to the soaking liquid, wherein the amount of animal Bifidobacterium is 10 6 -10 7 CFU / mL soaking liquid; ferment at 38-40℃ for 4-6h, sterilize, and continue to add Lactobacillus casei, the amount of Lactobacillus casei is 10 8 -10 9 CFU / mL soaking liquid, ferment at 38-40℃ for 3-5h, sterilize, and finally add Bifidobacterium bifidum. The dosage of Bifidobacterium bifidum is 10 7 -10 8 CFU / mL soaking solution, fermenting at 38-40°C for 2-4 hours, sterilizing to obtain a fermentation liquid, subjecting the fermentation liquid to steam distillation extraction for 2-4 hours, collecting and drying to obtain an oil component and a first extract; further purifying the first extract to obtain a water-soluble component; and mixing the water-soluble component and the oil component in a proportion to obtain a fennel extract;

[0011] S3: The fennel extract and the gardenia extract are mixed to obtain a gardenia and fennel composite extract.

[0012] Furthermore, the volume fraction of ethanol added in step S1 is 60%-75%.

[0013] Furthermore, the extraction in step S1 specifically includes: crushing and sieving the gardenia into coarse powder, adding deionized water, and extracting three times at a temperature of 40-50° C., each time for 1-2 hours to obtain an extract, and combining the extracts to obtain a first crude extract.

[0014] Furthermore, the weight ratio between the fennel powder and water in step S2 is 1:(7-9).

[0015] Furthermore, the extraction time of the steam distillation method in step S2 is 2-4 hours.

[0016] Furthermore, the macroporous adsorption resin column used in the purification process in step S2 is D101 type. Furthermore, the macroporous adsorption resin column used in the purification process in step S2 is D101 type, and the weight ratio between the water-soluble component and the oil component in step S2 is 1-3:4-6.

[0017] Furthermore, the weight ratio between the fennel extract and the gardenia extract in step S3 is 1:2 or 1:1.

[0018] The present invention compounds fennel extract and gardenia extract, and limits the weight ratio between the two to 1:2 or 1:1. The active ingredients in different extracts enhance each other's efficacy, reduce the occurrence of side effects, thereby achieving the effect of 1+1>2, and further improving the stability of the compound. Among them, the flavonoids in the fennel extract and the antioxidant components in the gardenia extract work together to enhance the overall antioxidant capacity, help protect pancreatic beta cells, promote normal insulin secretion, inhibit lipid peroxidation and inflammation, and reduce the levels of total cholesterol, triglycerides and low-density lipoprotein in serum, with significant lipid-lowering and blood sugar-lowering effects.

[0019] In addition, the present invention also provides a gardenia and fennel composite extract obtained by the above preparation method.

[0020] The present invention also provides the use of the composite extract of gardenia and fennel in the preparation of health products or medicines for lowering blood sugar and / or blood lipids.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] (1) By limiting the volume fraction of ethanol, the effective active ingredients in Gardenia jasminoides extract were increased.

[0023] (2) By limiting the fennel extract to include oil components and water-soluble components, the antioxidant activity of the compound is further improved, enabling it to better exert the effect of lowering blood lipids and blood sugar. At the same time, the weight ratio of fennel oil components to water-soluble components is limited to 1-3:4-6, which improves the stability of the compound and further enhances the synergistic effect of the compound.

[0024] (3) By compounding fennel extract and gardenia extract and limiting the weight ratio between the two to 1:2 or 1:1, the effect of 1+1>2 is achieved, and the stability of the compound is further improved, which has a significant effect of lowering blood lipids and blood sugar.

[0025] (4) The compound of the present invention has a significant regulating effect on the patient's blood pressure and blood lipids, and has the efficacy of improving the patient's blood pressure, blood lipids and blood sugar. It is non-toxic and harmless to the human body and has no side effects. DETAILED DESCRIPTION

[0026] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0027] The bacteria used in the present invention were purchased from the following manufacturers:

[0028] Lactobacillus acidophilus, accession number: CCTCC AB 2010208, was purchased from China Center for Type Culture Collection.

[0029] Bifidobacterium animalis, accession number: SHBCC D24407AS 1.1852, was purchased from China Center for Type Culture Collection.

[0030] Lactobacillus casei, accession number: SHBCC D24737, was purchased from Shanghai Microbiological Collection Center.

[0031] Bifidobacterium bifidum, accession number: SHBCC D24408 ATCC 35914, was purchased from Shanghai Microbiological Collection Center.

[0032] Bifidobacterium lactis, accession number: SHBCC D24675, was purchased from Shanghai Microbiological Collection Center.

[0033] Lactobacillus bulgaricus, accession number: CCTCC CB 20082295, was purchased from China Center for Type Culture Collection.

[0034] Example 1

[0035] This embodiment provides a method for preparing a composite extract of gardenia and fennel, the preparation method comprising the following steps:

[0036] S1: Preparation of Gardenia Extract: 10.00 kg of Gardenia was taken and crushed into coarse powder. 10 times the amount of water was added and extracted 3 times at 45°C for 1.5 h each time. The extracts were combined to obtain a first crude extract. Composite bacteria were added to the first crude extract. The composite bacteria included Lactobacillus acidophilus, Bifidobacterium lactis and Lactobacillus bulgaricus. The amount of Lactobacillus acidophilus was 10 6 CFU / mL first crude extract; the dosage of Bifidobacterium lactis is 10 8 CFU / mL first crude extract; the dosage of Lactobacillus bulgaricus is 10 9CFU / mL first crude extract; ferment at 39°C for 8 hours, sterilize to obtain a first fermentation liquid, add 75% ethanol of the first fermentation liquid volume fraction to the first fermentation liquid, let it stand for 24 hours, filter, recover ethanol from the filtrate and concentrate to a relative density of 1.20 at 70°C to obtain an extract, then add 5 times the amount of water of the extract, refrigerate for 48 hours, filter, concentrate the filtrate to a thick paste with a relative density of 1.28 at 70°C, vacuum dry, and grind to obtain;

[0037] S2: Preparation of fennel extract: 10.00 kg of fennel was crushed and sieved to obtain fennel powder. 8 times the weight of water was added to the fennel powder and soaked for 2 hours to obtain an immersion solution. Animal Bifidobacterium was added to the immersion solution. The amount of animal Bifidobacterium was 10 6 CFU / mL soaking liquid; ferment at 39℃ for 5h, sterilize, add Lactobacillus casei, the amount of Lactobacillus casei is 10 9 CFU / mL soaking liquid, ferment at 39℃ for 4h, sterilize, and continue to add Bifidobacterium bifidum. The amount of Bifidobacterium bifidum is 10 7 CFU / mL soaking liquid, fermented at 39℃ for 3h, sterilized to obtain fermentation liquid, extracted the fermentation liquid by steam distillation for 3h, dried to obtain 40mL of oil component and the first extract, then applied the first extract to D101 macroporous adsorption resin column (diameter-to-height ratio of 1:10), first removed impurities with 10 column volumes of water, then eluted with 8 column volumes of 60% ethanol, collected the 60% ethanol eluate, concentrated under reduced pressure of 0.05MPa, and vacuum dried under the conditions of negative pressure of 0.05MPa and temperature of 50℃ to obtain 66.87g of fennel water-soluble component powder, which is the fennel water-soluble component; the water-soluble component and the oil component were mixed in a weight ratio of 2:5 to obtain fennel extract;

[0038] S3: The fennel extract and the gardenia extract are mixed in a weight ratio of 1:2 to obtain a gardenia and fennel composite extract.

[0039] Example 2

[0040] The difference between this embodiment and embodiment 1 is that the volume fraction of ethanol added in step S1 is 60%.

[0041] Example 3

[0042] The difference between this embodiment and embodiment 1 is that the weight ratio between the water-soluble component and the oil component in step S2 is 3:6.

[0043] Example 4

[0044] The difference between this embodiment and embodiment 1 is that the weight ratio between the fennel extract and the gardenia extract in step S3 is 1:1.

[0045] Comparative Example 1

[0046] The difference between this comparative example and Example 1 is that the volume fraction of ethanol added in step S1 is 95%.

[0047] Comparative Example 2

[0048] The difference between this comparative example and Example 1 is that the weight ratio between the water-soluble component and the oil component in step S2 is 1:10.

[0049] Comparative Example 3

[0050] The difference between this comparative example and Example 1 is that the fennel extract does not include water-soluble components.

[0051] Comparative Example 4

[0052] The difference between this comparative example and Example 1 is that the gardenia and fennel composite extract does not include fennel extract.

[0053] Comparative Example 5

[0054] The difference between this comparative example and Example 1 is that the gardenia and fennel composite extract does not include the gardenia extract.

[0055] Comparative Example 6

[0056] The difference between this comparative example and Example 1 is that the weight ratio between the fennel extract and the gardenia extract in step S3 is 2:5.

[0057] Comparative Example 7

[0058] The difference between this comparative example and Example 1 is that the bacterial species are different: a composite bacteria is added to the first crude extract, the composite bacteria including animal Bifidobacterium, Lactobacillus casei and Bifidobacterium bifidum, and the amount of the animal Bifidobacterium is 10 6 CFU / mL first crude extract; the amount of Lactobacillus casei is 10 8 CFU / mL first crude extract; the dosage of Bifidobacterium bifidum is 10 9 CFU / mL first crude extract; ferment at 39°C for 8h, sterilize, and obtain the first fermentation broth.

[0059] Comparative Example 8

[0060] The difference between this comparative example and Example 1 is that the fermentation method is different: Lactobacillus acidophilus is added to the first crude extract, and the amount of Lactobacillus acidophilus is 10 6 CFU / mL of the first crude extract was fermented at 39°C for 3h, sterilized, and Bifidobacterium lactis was added. The amount of Bifidobacterium lactis was 10 8CFU / mL first crude extract; ferment at 39 ° C for 3h and sterilize; finally add Lactobacillus bulgaricus, the amount of Lactobacillus bulgaricus is 10 9 CFU / mL first crude extract; ferment at 39°C for 2h, sterilize, and obtain a first fermentation broth.

[0061] Comparative Example 9

[0062] The difference between this comparative example and Example 1 is that the strains are different: Lactobacillus acidophilus is added to the soaking liquid, and the amount of Lactobacillus acidophilus is 10 6 CFU / mL soaking liquid; ferment at 39℃ for 5h, sterilize, add Bifidobacterium lactis, the amount of Bifidobacterium lactis is 10 9 CFU / mL soaking liquid, ferment at 39℃ for 4h, sterilize, and continue to add Lactobacillus bulgaricus, the dosage of Lactobacillus bulgaricus is 10 7 CFU / mL soaking solution, fermentation at 39 ° C for 3 h, sterilization, and fermentation liquid.

[0063] Comparative Example 10

[0064] The difference between this comparative example and Example 1 is that the fermentation method is different: a composite bacteria is added to the soaking liquid, the composite bacteria includes animal Bifidobacterium, Lactobacillus casei and Bifidobacterium bifidum, and the amount of animal Bifidobacterium is 10 6 CFU / mL soaking solution; the dosage of Lactobacillus casei is 10 9 CFU / mL soaking solution, the dosage of Bifidobacterium bifidum is 10 7 CFU / mL soaking solution, fermentation at 39 ° C for 12 h, sterilization, and fermentation liquid.

[0065] Performance Testing

[0066] 1. Pharmacological Experiments

[0067] (1) Experimental materials

[0068] Samples: extracts from Examples 1-4 and extracts from Comparative Examples 1-10;

[0069] Experimental animals: Male ICR mice weighing 30-40 g were housed in a room at 25°C and 55°C with free access to food and water. The mice were acclimated for one week. The basal feed was a commercially available high-fat diet.

[0070] (2) Model building

[0071] After one week of acclimatization, mice, except for a control group (10 normal mice), were fasted for 12 hours and then intraperitoneally injected with 130 mg / kg STZ (dissolved in freshly prepared 0.1 M citrate buffer, pH 4.5). 72 hours later, fasting blood glucose (FBG) was measured. Mice with blood glucose levels ≥7.80 mmol / L were considered successful model mice and used in subsequent experiments. The 120 successfully modeled mice were randomly divided into 12 groups: a model control group (fed with an equal volume of saline), a metformin group (fed with 250 mg / kg metformin), and a sample group (10 mice each). Each single and combined drug component was dried, ground, and passed through a 40-mesh sieve. All drugs were suspended in 0.5% sodium carboxymethylcellulose (CMC-Na) and administered orally at a volume of 0.2 mL / 10 g (equivalent to a dose of 250 mg / kg). The model group received an equal volume of CMC-Na solution.

[0072] The mice were dosed once daily between 8:30 and 9:30 a.m. for 4 consecutive weeks. The body weight of the mice was recorded before daily dosing.

[0073] (3) Determination of fasting blood glucose and insulin in mice;

[0074] After four weeks of drug administration, fasting blood glucose was measured in each group of mice. After fasting for 12 hours, blood was collected from the retro-orbital venous plexus of the mice and centrifuged at 3500 rpm for 10 minutes to separate the serum. Serum was then loaded onto a 96-well plate using the glucose kit method and measured with a microplate reader. Fasting blood glucose and insulin levels were calculated.

[0075] (4) Determination of blood lipid indicators in mice

[0076] After 4 weeks of administration, blood was collected from the eyeballs of mice in each group and centrifuged at 3500 r / min for 10 min to separate the serum. The samples were loaded into 96-well plates according to the TC, TG, HDL-C, and LDL-C kit methods and measured with a microplate reader to calculate the serum TC, TG, and LDL-C levels of the mice.

[0077] (5) Data statistics;

[0078] The data were expressed as mean ± standard deviation (mean ± SD). SPSS 17.0 software was used for statistical analysis.

[0079] *Indicates significant difference compared with the model control group (P<0.05);

[0080] ** indicates extremely significant difference compared with the model control group (P<0.01);

[0081] # indicates significant difference compared with the normal group (P<0.05);

[0082] ## indicates extremely significant difference compared with the normal group (P<0.01);

[0083] Δ indicates significant difference compared with Examples 1-4 (P < 0.05);

[0084] ΔΔ indicates that the difference compared with Examples 1-4 is extremely significant (P<0.01).

[0085] 2. Experimental Results

[0086] 2.1 Effects on fasting blood glucose and insulin levels in type 2 diabetic mice

[0087] As shown in Table 1, after four weeks of administration, compared with the mice in the model control group, Examples 1-4 all significantly reduced the fasting blood glucose values ​​of the mice, while the blood glucose values ​​of the mice in Comparative Examples 1-6 increased significantly, especially when the composite extract did not contain Gardenia extract, the fasting blood glucose values ​​of the mice increased significantly, indicating that the compound of the present application can significantly reduce the fasting blood glucose values ​​of the mice, especially the compound containing no Gardenia extract or no Fennel extract in Comparative Examples 4-5, which significantly increased the fasting blood glucose values ​​in the mice, and the difference was significant compared with Example 1, which proves that the compound containing Gardenia extract and Fennel extract can play a role in lowering blood glucose. , and Comparative Example 6 also proves that the compound ratio between gardenia extract and fennel extract has a great influence on the fasting blood glucose value of mice. In addition, Comparative Examples 2 and 3 also prove that the compound ratio between the water-soluble component and the oil component in fennel extract, as well as the water-soluble component and the oily component in fennel extract, can have the technical effect of significantly lowering blood glucose. Comparative Examples 7-10 prove that the strains and fermentation methods used in the preparation of gardenia extract and fennel extract will affect the effect of the composite extract in lowering blood glucose. In summary, the compound of gardenia extract and fennel extract in the present application can significantly lower the fasting blood glucose of mice.

[0088] As shown in Table 2, through four weeks of treatment, compared with the mice in the model control group, the insulin content of the mice in the metformin group obviously rises, showing that the mice induced by STZ have produced the characteristics such as insulin resistance and insulin secretion deficiency. After administration four weeks, the insulin content of the mice in embodiment 1-4 groups significantly improves, showing that extract can improve the fasting insulin level of diabetic mice, and has statistical significance, compared with embodiment 1-4 groups, the fasting insulin level of the mice in comparative example 1-6 groups has a certain degree of reduction, showing that the weight ratio between the water-soluble component and the oil component in the fennel extract, the weight ratio between the fennel extract and the gardenia extract and other conditions all have an impact on the drug effect of the extract, and if the gardenia composition is not added in the extract or the water-soluble component is not added, the hypoglycemic effect of the extract can be caused to a significant impact. The bacterial species and the fermentation method used in the fermentation of comparative example 7-10 are changed, and the hypoglycemic effect of the extract can also be caused to a significant impact.

[0089] Table 1: Effects on fasting blood glucose (FBG) in type 2 diabetic mice

[0090]

[0091] Table 2: Effects on insulin in type 2 diabetic mice

[0092]

[0093] 2.2 Effects on blood lipids in type 2 diabetic mice

[0094] Test results: As shown in Table 3, the serum TC, TG and LDL-C levels of mice are important indicators of hyperlipidemia. After four weeks of drug administration, the TC, TG and LDL-C levels in the serum of the mice in Examples 1-4 were significantly reduced, while the content of each component in Comparative Example 1 showed an upward trend. This may be due to the low volume fraction and high water content in the ethanol extract, which in turn resulted in a lower content of crocin in the gardenia extract, reducing the lipid-lowering effect of the composite extract. The composites in Comparative Examples 4-5 that did not contain gardenia extract or fennel extract, and the composites in Comparative Example 6 that regulated the compounding ratio between gardenia extract and fennel extract, significantly increased the TC, TG and LDL-C levels in the serum of mice. This may be due to the compounding between fennel extract and gardenia extract, while limiting the weight ratio of the two to a certain range, thereby achieving the effect of 1+1>2. The flavonoids in the fennel extract and the fennel extract were significantly increased. The antioxidant components in the gardenia extract work together to enhance the overall antioxidant capacity, help protect pancreatic beta cells, promote normal insulin secretion, inhibit lipid peroxidation and inflammation, and reduce the levels of total cholesterol, triglycerides, and low-density lipoprotein in serum, with significant lipid-lowering and blood sugar-lowering effects. The TC, TG, and LDL-C levels in the serum of mice in Comparative Examples 2 and 3 were also significantly reduced, indicating that the water-soluble components and oil components in the fennel extract can have a certain effect on the blood lipids of mice. At the same time, the fennel extract with water-soluble components and oil components and the gardenia extract compound have a significant synergistic effect, mainly because the active components such as anethole contained in the fennel oil component are compatible with the active components such as flavonoids in the water-soluble component, which can further enhance the synergistic effect of the compound. The TC, TG, and LDL-C levels in the serum of mice in Comparative Examples 7-10 were also significantly reduced.

[0095] Table 3: Effects on blood lipid indexes in type 2 diabetic mice

[0096]

[0097] In summary, the composite extract of gardenia and fennel of the present invention can significantly reduce the fasting blood glucose value of mice, increase the insulin content of mice, and also significantly reduce the TC, TG and LDL-C contents of mice. This proves that the flavonoids in the fennel extract and the antioxidant components in the gardenia extract work together to enhance the overall antioxidant capacity, help protect pancreatic β cells, promote normal insulin secretion, inhibit lipid peroxidation and inflammation, and reduce the levels of total cholesterol, triglycerides and low-density lipoprotein in serum, thereby having a significant lipid-lowering and blood sugar-lowering effect.

[0098] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a composite extract of Gardenia jasminoides and Fennel, characterized in that: The following steps are involved: S1: Preparation of Gardenia Extract: Grind and sieve Gardenia into coarse powder, add water and extract several times to obtain extract, combine the extracts to obtain a first crude extract; add composite bacteria to the first crude extract, the composite bacteria including Lactobacillus acidophilus, Bifidobacterium lactis and Lactobacillus bulgaricus, the amount of Lactobacillus acidophilus is 10 6 -10 7 CFU / mL first crude extract; the dosage of Bifidobacterium lactis is 10 7 -10 8 CFU / mL first crude extract; the dosage of Lactobacillus bulgaricus is 10 8 -10 9 CFU / mL first crude extract; Fermenting at 38-40°C for 7-9 hours and sterilizing to obtain a first fermentation broth, adding 60%-75% ethanol by volume to the first fermentation broth for extraction to obtain an ethanol extract, allowing to stand, filtering, and concentrating until no alcohol tastes, adding water, refrigerating, and filtering, concentrating the filtrate to obtain a thick paste, and drying and crushing to obtain a gardenia extract; S2: Preparation of fennel extract: Fennel powder and water were mixed to obtain a soaking solution, and animal Bifidobacterium was added to the soaking solution. The amount of animal Bifidobacterium was 10 6 -10 7 CFU / mL soaking solution; ferment at 38-40℃ for 4-6h, sterilize, and continue to add Lactobacillus casei; Dosage of Lactobacillus casei 10 8 -10 9 CFU / mL soaking liquid, ferment at 38-40℃ for 3-5h, sterilize, and finally add Bifidobacterium bifidum. The dosage of Bifidobacterium bifidum is 10 7 -10 8 CFU / mL soaking liquid; fermenting at 38-40°C for 2-4 hours, sterilizing to obtain a fermentation liquid, subjecting the fermentation liquid to steam distillation extraction, collecting and drying to obtain an oil component and a first extract; purifying the first extract to obtain a water-soluble component; mixing the water-soluble component and the oil component in a proportion to obtain a fennel extract, wherein the weight ratio of the water-soluble component to the oil component is 1-3:4-6; S3: The fennel extract and the gardenia extract are mixed to obtain a gardenia-fennel composite extract, wherein the weight ratio of the fennel extract to the gardenia extract is 1:2 or 1:

1.

2. The method for preparing a composite extract of Gardenia jasminoides and Fennel according to claim 1, wherein: The extraction in step S1 specifically includes: crushing and sieving gardenia into coarse powder, adding deionized water, and extracting three times at a temperature of 40-50° C., each time for 1-2 hours to obtain an extract, and combining the extracts to obtain a first crude extract.

3. The method for preparing a composite extract of Gardenia jasminoides and Fennel according to claim 1, wherein: The weight ratio between the fennel powder and water in step S2 is 1:7-9.

4. The method for preparing a composite extract of Gardenia jasminoides and Fennel according to claim 1, wherein: The extraction time by steam distillation in step S2 is 2-4 hours.

5. The method for preparing a composite extract of Gardenia jasminoides and Fennel according to claim 1, wherein: The macroporous adsorption resin column used in the purification treatment in step S2 is of type D101.

6. A composite extract of Gardenia jasminoides and Fennel, characterized by: A composite extract of gardenia and fennel obtained by the preparation method according to any one of claims 1 to 5.

7. Use of the composite extract of Gardenia jasminoides and Fennel according to claim 6 in the preparation of health products or medicines for lowering blood sugar and / or blood lipids.

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