A fermentation active ingredient with soothing and repairing properties and its preparation method

Abstract

This invention relates to the field of cosmetic technology, specifically providing a fermented active ingredient with soothing and repairing effects, its preparation method, and its application. The fermented active ingredient is prepared through a two-stage fermentation process using a compound microbial strain (Lactobacillus plantarum CGMCC1.557:Pichia pastoris ATCC76273 = 3:1) of sea cabbage extract, kava extract, and sea fennel extract: first, anaerobic fermentation at 28°C for 72 hours to pH 5.5, followed by microaerobic fermentation at 32°C for 48 hours to pH 6.8, and then sterilization by ultrafiltration. This active ingredient is compounded with rhamnose, pentylene glycol, and hexanediol to form a plant-based complex essence. The active ingredient is released slowly through a rhamnose-pentylene glycol hydrogen bond network, and the pentylene glycol-hexanediol system provides low-allergenic preservative. Experiments show that this essence can reduce the area of ​​erythema by 47.1-53.6%, increase the stratum corneum moisture content by 59.4-65.9%, shorten the redness fading time to 2-5 minutes, and is non-allergenic, significantly superior to single-plant extract compound formulations.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a fermented active ingredient with soothing and repairing properties and its preparation method. Background Technology

[0002] In the field of cosmetic technology, serums, as a core skincare product, are primarily used to soothe skin redness and swelling, reduce inflammation, and repair damage, helping the skin return to a healthy and firm state. Their efficacy mainly relies on plant extracts as active ingredients, which work through a water-based carrier. However, significant shortcomings exist in existing technologies, resulting in less than ideal repair and soothing effects from serums, and also posing safety risks.

[0003] Specifically, current serums generally employ high-concentration combinations of various plant extracts to achieve multi-functional repair effects. For example, patent CN109549893B describes a serum containing multiple ingredients, including purslane extract, chlorella extract, sea cabbage extract, ginger flower root extract, and spring elm extract. This strategy of adding large amounts of ingredients not only significantly increases production costs but also leads to poor skin absorption due to the low biocompatibility of plant extracts. Despite the increased active ingredients, the repair and soothing effects have not shown a positive correlation; on the contrary, high concentrations of active ingredients require the addition of stronger antibacterial and preservative ingredients, which may pose a threat to skin safety, such as causing irritation or allergic reactions.

[0004] Furthermore, common active ingredients such as sea kale extract, kava extract, and sea fennel extract have limited efficacy and require high concentrations in existing technologies. For example, patent CN108553596A's soothing composition contains 10-18% sea fennel extract; this high dose can easily irritate the skin, but its repairing and soothing effects are not significant. Similarly, while sea kale extract (containing glucosinolates, with anti-aging and anti-inflammatory properties), kava extract (containing kava lactone, which can regulate neuroinflammatory reactions), and sea fennel extract (promoting keratinocyte regeneration and anti-oxidation) each have their advantages, their compatibility issues prevent synergistic effects when used alone or in simple combinations, potentially leading to increased skin burden.

[0005] To verify these shortcomings, existing technologies often employ experimental methods to evaluate serum performance. For example, inducing skin redness and swelling through histamine or other irritants (such as histamine phosphate solution): one method involves applying a placebo, serum, or steroid cream before applying the irritant and observing the time it takes for redness and swelling to subside; another method involves applying the irritant to induce redness and swelling before applying the test sample to evaluate the soothing effect. These experiments highlight the shortcomings of existing serums in rapid redness reduction and repair efficiency. For instance, test results show that traditional serums take 8-10 minutes to reduce redness, far below the ideal effect. Simultaneously, rhamnose, as a potential synergistic ingredient, has not had its sustained-release efficacy fully utilized in existing technologies, further limiting the optimization of serum performance.

[0006] In summary, existing serum technology faces three core challenges:

[0007] (1) Poor economic efficiency: It relies on a variety of high-concentration plant extracts, resulting in high costs;

[0008] (2) Low efficacy: Low biocompatibility leads to insufficient absorption rate, and the repair effect is not proportional to the concentration of ingredients;

[0009] (3) Safety risks: Highly active ingredients require strong preservatives, which may increase the possibility of skin irritation and sensitization.

[0010] These problems urgently require an innovative solution that optimizes ingredient combinations and preparation processes to achieve efficient, safe, and economical soothing and repairing effects. Summary of the Invention

[0011] To address the aforementioned problems and achieve the aforementioned objectives, this invention first provides a fermentation active ingredient, prepared by fermentation of a compound of sea cabbage extract, kava extract, and sea fennel extract in a mass ratio of 4-7:4-6:4-7 using a complex microbial strain.

[0012] In some embodiments, the composite strain is composed of Lactobacillus plantarum CGMCC1.557 and Pichia pastoris ATCC76273 in a 3:1 ratio, with an inoculation amount of 1.5 ± 0.2% of the mass of the mixture.

[0013] The present invention also provides a method for preparing the above-mentioned fermentation active ingredient, comprising:

[0014] S1. Mix the three extracts together and inoculate them with a compound bacterial strain;

[0015] S2.28℃±1℃ anaerobic fermentation for 72 hours until pH=5.5;

[0016] S3.32℃±1℃ microaerobic fermentation for 48 hours until pH=6.8;

[0017] S4. Ultrafiltration through a 10kDa membrane followed by sterilization.

[0018] In some embodiments, a method for preparing a fermentation-active substance includes the steps of:

[0019] S1. Mix sea cabbage extract, kava extract and sea fennel extract in a mass ratio of 4-7:4-6:4-7 (preferably 1:1:1), and inoculate the mixture with microbial inoculum at a concentration of 1.5 ± 0.2% of the mixture.

[0020] The strain is a compound strain, consisting of Lactobacillus plantarum CGMCC 1.557 and Pichia pastoris ATCC 76273 in a ratio of 3:1.

[0021] S2, First fermentation stage: The mixture inoculated with the inoculum is anaerobic fermented at 28℃±1℃ for 72 hours until the pH of the mixture is 5.5;

[0022] S3, Second fermentation stage: The mixture that has undergone the first fermentation stage is fermented at 32℃±1℃ under microaerobic conditions for 48 hours to make the pH of the mixture 6.8;

[0023] S4. The fermented mixture is subjected to ultrafiltration (10kDa membrane) and sterilization to obtain the fermentation active product.

[0024] The present invention also provides a plant compound essence, comprising 6.0-10.0% of the above-mentioned fermented active ingredients, 2.0-4.0% of rhamnose, 3.5-5.0% of pentanediol, 0.3-0.5% of hexanediol, and the balance being water.

[0025] In some embodiments, the fermentation active ingredient is 7.5%, rhamnose is 2.5%, pentylene glycol is 5.0%, and hexanediol is 0.5%.

[0026] In some embodiments, rhamnose forms a sustained-release hydrogen bond network with pentanediol.

[0027] In some embodiments, pentylene glycol and hexanediol constitute a polyol preservative system.

[0028] The present invention also provides a method for preparing the above-mentioned essence, comprising:

[0029] S1. Dissolve rhamnose in 60℃ warm water (30% of the total water volume);

[0030] S2. Add pentylene glycol and hexanediol, and homogenize at 2000 rpm for 5 minutes;

[0031] S3. Add fermentation active ingredients and add water to 100%.

[0032] This invention also provides the application of the above-mentioned fermentation active ingredients in the preparation of soothing and repairing cosmetics.

[0033] This invention also provides the application of the above-mentioned essence in repairing ultraviolet damage.

[0034] Among them, sea cabbage extract contains glucosinolates, which can inhibit TRPV1 receptors and promote the production of glutathione. It has skin-revitalizing effects and the ability to scavenge free radicals. The extract has excellent anti-aging and anti-inflammatory properties.

[0035] Kava extract contains kavalactone, which has various pharmacological effects such as antidepressant, sedative, antibacterial, and analgesic properties, and can regulate neuroinflammatory conditions.

[0036] Sea fennel extract can promote the regeneration of keratinocytes, help maintain the integrity of skin structure and moisture balance, and improve skin elasticity and firmness. Furthermore, the polyphenolic compounds in it have strong antioxidant activity, capable of scavenging free radicals and inhibiting oxidative stress responses.

[0037] The synergistic combination of rhamnose and pentylene glycol, a sugar-polyol combination, forms a molecular hydrogen bond network, which can prolong the sustained release of the active ingredient.

[0038] The pentylene glycol-hexanediol polyol preservative system has good antiseptic and antibacterial effects, soothes the skin, and has low allergenicity.

[0039] Compared with the prior art, the present invention has at least the following beneficial effects:

[0040] (1) Synergistic effect: The fermentation process improves the conversion rate of active ingredients in sea cabbage (containing glucosinolates), kava (kava lactone), and sea fennel (polyphenols), and the erythema repair rate is 200% higher than that of single extracts.

[0041] (2) Safe sustained release: The rhamnose-pentanediol combination prolongs the action time of the active ingredient, and the pentanediol-hexanediol system replaces traditional preservatives, with a zero sensitization rate;

[0042] (3) Rapid relief: In the histamine phosphate stimulation test, the redness reduction time was shortened to 2 minutes and 12 seconds, which is close to the effect of hormone ointment;

[0043] (4) Barrier repair: In the UV damage repair experiment, the density of Langerhans cells increased by 8 times, confirming the enhancement of the immune barrier. Attached Figure Description

[0044] Figure 1 This is a flowchart illustrating the preparation process of the fermentation active ingredient of the present invention. Detailed Implementation

[0045] To make the technical problems, technical solutions and advantages of the present invention clearer, a detailed description will be given below in conjunction with the accompanying drawings and specific embodiments.

[0046] Example 1

[0047] Methods for preparing fermentation active substances include:

[0048] S1. Mix sea cabbage extract, kava extract and sea fennel extract in a mass ratio of 4-7:4-6:4-7 (preferably 1:1:1), and inoculate the mixture with microbial inoculum at a concentration of 1.5 ± 0.2% of the mixture.

[0049] The strain is a compound strain, consisting of Lactobacillus plantarum CGMCC 1.557 and Pichia pastoris ATCC 76273 in a ratio of 3:1.

[0050] S2, First fermentation stage: The mixture inoculated with the inoculum is anaerobic fermented at 28℃±1℃ for 72 hours until the pH of the mixture is 5.5;

[0051] S3, Second fermentation stage: The mixture that has undergone the first fermentation stage is fermented at 32℃±1℃ under microaerobic conditions for 48 hours to make the pH of the mixture 6.8;

[0052] S4. The fermented mixture is subjected to ultrafiltration (10kDa membrane) and sterilization to obtain the fermentation active product.

[0053] The plant-based complex essence in this embodiment has the following composition:

[0054] Raw material usage (g)

[0055] Fermentation active ingredient 8.0 (Sea cabbage extract: kava extract: sea fennel extract = 4:5:7)

[0056]

[0057] Example 2

[0058] The plant-based complex essence in this embodiment has the following composition:

[0059] Example 3

[0060] The plant-based complex essence in this embodiment has the following composition:

[0061] Example 4

[0062] The plant-based complex essence in this embodiment has the following composition:

[0063]

[0064]

[0065] Example 5

[0066] The plant-based complex essence in this embodiment has the following composition:

[0067]

[0068] The preparation of the plant compound essences of Examples 1-5 includes the following steps:

[0069] S1. Take 30% of the total water volume and heat it to 60°C. Dissolve the rhamnose in the 60°C warm water.

[0070] S2. Add pentylene glycol and hexanediol, and homogenize at 2000 rpm for 5 min;

[0071] S3. Add fermentation active ingredients;

[0072] S4, Replenish water to 100%.

[0073] Comparative Example 1

[0074] Compared to Example 2, sea cabbage extract was used to replace the fermentation active ingredient.

[0075] Comparative Example 2

[0076] Compared to Example 2, sea fennel extract was used to replace the fermentation active ingredient.

[0077] Comparative Example 3

[0078] Compared to Example 2, kava extract was used to replace the fermentation active ingredient.

[0079] Comparative Example 4

[0080] Compared with Example 2, a compound of sea cabbage extract, sea fennel extract, and kava extract was used to replace the fermentation active ingredient.

[0081] Comparative Example 5

[0082] Compared to Example 2, hexanediol was replaced with p-hydroxyacetophenone.

[0083] Comparative Example 6

[0084] Compared to Example 2, only the first fermentation stage was performed.

[0085] Comparative Example 7

[0086] Compared to Example 2, only the second fermentation stage was performed.

[0087] Comparative Example 8

[0088] Compared to Example 2, water was used instead of rhamnose.

[0089] The serums from Examples 1-5 and Comparative Examples 1-8 were tested for use, as follows.

[0090] Repair test

[0091] Test subjects: 13 female volunteers aged 30-35 with no history of skin diseases.

[0092] Test method: Thirteen volunteers were divided into 13 groups, and the 13 volunteers respectively used the essence of Examples 1-5 and Comparative Examples 1-8 for testing.

[0093] In this study, a 2cm x 2cm test area was selected on the left forearm of each volunteer and exposed to ultraviolet light using a UV phototherapy device [model SQ308PCNFD, Jiangsu Sigma Medical Technology Co., Ltd.] at an intensity of 0.30mW / cm². 2 To simulate the formation of sunburn.

[0094] After sunburn occurred in the test area, each volunteer used the corresponding embodiment / comparison ratio of the serum to soothe and repair the burn. They used it three times a day, morning, noon and evening, applying 3g of the corresponding serum evenly to the test area each time, for 7 consecutive days.

[0095] Test Results: The changes in erythema area in the test areas of each volunteer before and after using the serum were compared, and the skin moisture content was measured using a skin moisture meter [MDD4-]. [CM825] The moisture changes in the test area before and after using the serum were measured. The test results are shown in Table 1 below.

[0096] Table 1. Test Results

[0097]

[0098]

[0099] Soothing test

[0100] Test subjects: 14 female volunteers aged 30-35 with no history of skin diseases.

[0101] Test method: 14 volunteers were divided into 14 groups, and the 14 volunteers respectively used the essence of Examples 1-5 and Comparative Examples 1-8 and the hormone ointment [prednisolone acetate ointment, Wuhan Dior Pharmaceutical Co., Ltd., National Drug Approval Number H42022574] for the relief test.

[0102] In this study, a 2cm x 2cm skin area was selected on the right forearm of each volunteer for testing.

[0103] (1) Take the corresponding test sample [3g of essence and 1g of hormone ointment] and apply it to the test area for a pre-experiment comfort; after the test sample is fully absorbed by the skin, take 0.05mL of 0.2% histamine phosphate solution prepared with saline and apply it to the test area to cause redness and swelling. Then observe the time required for the redness and swelling to subside. The redness and swelling subside time is shown in Table 2.

[0104] Table 2. Time for Redness and Swelling to Subside

[0105]

[0106]

[0107] (2) Apply 0.05 mL of 0.2% histamine phosphate solution prepared with saline to the test area to induce redness and swelling. Apply the corresponding test sample [3 g of essence and 1 g of hormone ointment] to the test area to soothe the redness and swelling. The time for redness and swelling to subside is shown in Table 3.

[0108] Table 3. Time for Redness and Swelling to Subside

[0109]

[0110] User Reviews

[0111] Based on the volunteers' use of the corresponding serums, a comprehensive evaluation of the serums in Examples 1-5 and Comparative Examples 1-8 was conducted. The scoring system ranged from 1 to 10 points, with higher scores indicating better results. Evaluation criteria included assessment of blemish removal, hydration, sensitization, and skin feel. The results are shown in Table 4.

[0112] Table 4. Evaluation Results

[0113]

[0114] animal experiments

[0115] Sixty-five 6-week-old female SPF-grade mice (Beijing Huafukang Biotechnology Co., Ltd.), weighing 25±2 grams, were used and divided into 13 groups of 5 mice each. Two 0.5cm×0.5cm skin areas were selected on the back of each mouse as test sites. The corresponding serum sample was applied to one test site, while the other test site was left untreated. Both test sites were then irradiated with ultraviolet light (irradiation intensity 2.50mW / cm²). 2 Skin tissue specimens were removed, the dermis and epidermis were separated, and the Langerhans cells of the epidermis were stained. The stained specimens were observed under a microscope. Five discontinuous fields of view were randomly selected from each slide under 400x light, and the number of positive cells in each square was counted. The cell density per unit area (cells / mm²) was calculated. 2The average value of the cell density calculated from 5 discontinuous fields of view is taken.

[0116] Table 5. Average cell density

[0117]

[0118] In summary, the plant-based compound essences in Examples 1-5 of this technical solution have good soothing and repairing effects and are highly safe to use.

Claims

1. A plant-based compound essence, characterized in that, The plant compound essence is composed of 6.0-10.0% fermentation active ingredients, 2.0-4.0% rhamnose, 3.5-5.0% pentanediol, 0.3-0.5% hexanediol, and the balance being water, by mass ratio. The method for preparing the fermentation active ingredient includes the following steps: S1. Mix sea cabbage extract, kava extract and sea fennel extract in a mass ratio of 4-7:4-6:4-7 and inoculate with compound microbial culture. S2, anaerobic fermentation at 28℃±1℃ for 72 hours until pH=5.5; S3, 32℃±1℃ microaerobic fermentation for 48 hours until pH=6.8; S4. Ultrafiltration through a 10kDa membrane followed by sterilization; The compound microbial strain consists of Lactobacillus plantarum CGMCC1.557 and Pichia pastoris ATCC76273 in a 3:1 ratio, with an inoculation amount of 1.5 ± 0.2% of the mixture's mass.

2. The plant compound essence according to claim 1, characterized in that: The plant compound essence is composed of 7.5% fermentation active ingredients, 2.5% rhamnose, 5.0% pentanediol, 0.5% hexanediol, and the balance being water, by mass ratio.

3. A method for preparing the plant compound essence according to claim 1, characterized in that: Includes the following steps: S1. Dissolve rhamnose in warm water at 60°C, wherein the rhamnose accounts for 30% of the total water volume; S2. Add pentylene glycol and hexanediol, and homogenize at 2000 rpm for 5 minutes; S3. Add the fermentation active ingredient as described in claim 1, and add water to 100%.

4. The application of the plant compound essence according to claim 1 in the preparation of soothing and repairing cosmetics.

5. The application of the plant compound essence according to claim 1 in repairing ultraviolet damage.

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