Method for producing yeast for feed by using erythritol concentrated phase

Abstract

The invention discloses a method for producing yeast for feed by using an erythritol concentrated phase, and belongs to the technical field of erythritol concentrated phase treatment. According to the method for producing the yeast for the feed by using the erythritol concentrated phase disclosed by the invention, the saccharomyces cerevisiae is added into the erythritol concentrated phase, maltose and maltotriose in the saccharomyces cerevisiae are decomposed and utilized, the content of thalli can be increased, and dry bacterial powder obtained after ceramic membrane filtration, roller drying and crushing is added into animal feed, so that the yeast for the feed is obtained. Effective utilization of concentrated phase resources can be achieved, and meanwhile environmental pollution is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of erythritol concentrated phase processing, and more particularly to a method for producing yeast for feed by utilizing erythritol concentrated phase. Background Art

[0002] In the production of erythritol, the fermentation method is usually used. In addition to erythritol, the fermentation broth also contains various impurities such as bacteria, pigments, proteins, inorganic salts, glucose, etc. When using a ceramic membrane for separation, under pressure, small molecules such as erythritol in the fermentation broth pass through the ceramic membrane to become permeate, while impurities such as bacteria, large molecular proteins, pigments, etc. are retained by the ceramic membrane. These retained substances are the concentrated phase of the erythritol ceramic membrane. After testing, the soluble solids content in the concentrated phase of the erythritol ceramic membrane is 10.0-13.0%, the pH is 3.9-4.1, and the conductivity is 2.0-3.0ms / cm, of which reducing sugars account for 10.0-15.0% of the soluble solids content.

[0003] After ceramic membrane pretreatment, the erythritol fermentation broth entering the nanofiltration membrane removes most of the large impurities, such as bacterial cells, but still contains some small molecules of protein, pigments, polysaccharides, and other impurities. The nanofiltration membrane has a high retention rate for these impurities, and the retained substances are the concentrated phase of the erythritol nanofiltration membrane. Testing shows that the soluble solids content of the concentrated phase of the erythritol nanofiltration membrane is 20.0-25.0%, the pH is 3.9-4.1, and the conductivity is 3.0-5.0ms / cm. Reducing sugars (maltose and maltotriose) account for 60.0-70.0% of the soluble solids.

[0004] In the method for reusing discarded yeast from erythritol production to prepare sprayed corn husks, patent CN117243296A describes a method for reusing discarded yeast from erythritol production to prepare sprayed corn husks. The Candida lipolytica cells are filtered through a ceramic membrane and then a polyalumina flocculant is added. The cells are separated using a disc separator to obtain a sprayed yeast liquid. The sprayed yeast liquid is mixed with corn husk fiber, dried, and packaged to prepare high-value sprayed corn husks for use as animal feed. In patent CN118716462A, a method for preparing animal feed using fermented litchi pomace and probiotics is described. The fermented litchi pomace and feed are evenly mixed, and then Candida lipolytica and prebiotics are added. The animal feed is then fermented for 24 to 48 hours at 25 to 30°C. This indicates that the erythritol production residue (Candida lipolytica) can be used to prepare animal feed.

[0005] Adding brewer's yeast to animal feed can enhance animal growth performance, improve intestinal health, and enhance immunity. It has a wide range of applications in ruminants (cattle, sheep), poultry (chickens, ducks), and aquaculture.

[0006] Erythritol ceramic membranes and nanofiltration membrane concentrated phases are generally treated as waste sewage. Due to their high COD and conductivity, they often have an impact on the sewage treatment system and also cause waste of reducing sugars and bacteria.

[0007] Therefore, providing a method for producing feed yeast using erythritol concentrated phase is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0008] In view of this, the present invention provides a method for producing yeast for feed using erythritol concentrated phase. By adding brewer's yeast to the erythritol concentrated phase, the maltose and maltotriose therein are decomposed and utilized, which can increase the bacterial content. The dry bacterial powder obtained after ceramic membrane filtration, drum drying, and crushing is added to animal feed, which can achieve effective utilization of concentrated phase resources while avoiding environmental pollution.

[0009] In order to achieve the above object, the present invention adopts the following technical solutions:

[0010] A method for producing yeast for feed using erythritol concentrated phase comprises the following steps:

[0011] (1) Preparation of erythritol concentrated phase: The erythritol ceramic membrane concentrated phase obtained by fermentation of Candida lipolytica and the erythritol nanofiltration membrane concentrated phase were mixed in a volume ratio of 1:1-2 to obtain an erythritol concentrated phase mixture;

[0012] (2) fermentation with brewer's yeast: the erythritol concentrated phase mixture is adjusted to a pH of 5.0-5.5 with a 4% sodium hydroxide solution, and the fermentation is carried out by inoculating brewer's yeast with an initial OD value of 15-25, with an inoculation amount of 1.0-1.5%; the fermentation conditions are controlled as follows: temperature 29-31°C, stirring speed 30-50 r / min, dissolved oxygen 5-10%; fermentation is carried out for 30-36 hours, and the residual sugar is detected by titration method to be less than 0.2%, and the fermentation liquid is removed from the tank to obtain the fermentation liquid;

[0013] (3) Ceramic membrane filtration: The fermentation liquid in the lower tank is filtered through a ceramic membrane to obtain a new ceramic membrane concentrated phase; pure water is added to the new ceramic membrane concentrated phase for washing, and the washing is stopped when the soluble solid content of the ceramic membrane permeate is ≤0.5 to obtain a mixture of Candida lipolytica and Saccharomyces cerevisiae;

[0014] (4) Drum drying and crushing: The mixture of Candida lipolytica and Saccharomyces cerevisiae is dried using a drum dryer, and the dried yeast bodies are crushed using a crusher to obtain dry feed yeast.

[0015] Furthermore, the soluble solid content in the concentrated phase of the erythritol ceramic membrane in step (1) is 10.0-13.0%, the pH is 3.9-4.1, and the conductivity is 2.0-3.0 ms / cm, wherein reducing sugars account for 10.0-15.0% of the soluble solid content;

[0016] The soluble solid content in the concentrated phase of the erythritol nanofiltration membrane is 20.0-25.0%, the pH is 3.9-4.1, and the conductivity is 3.0-5.0 ms / cm; and the reducing sugar accounts for 60.0-70.0% of the soluble solid content.

[0017] Furthermore, the pore size of the ceramic membrane in step (3) is 0.1 μm.

[0018] Furthermore, the drying temperature of the drum dryer in step (4) is 110-115°C.

[0019] Furthermore, the obtained feed yeast is added to animal feed in proportion. The proportion of feed yeast added to animal feed is 10-15 kg / ton for livestock feed, 40-50 kg / ton for poultry feed, and 40-50 kg / ton for fish and shrimp feed.

[0020] It can be seen from the above technical solution that, compared with the prior art, the present invention discloses a method for producing yeast for feed using erythritol concentrated phase. Saccharomyces cerevisiae is creatively added to the erythritol concentrated phase mixed liquid for fermentation, disaccharides, trisaccharides, etc. in the nanofiltration membrane concentrated phase are utilized, the Saccharomyces cerevisiae cell body is increased by 8-10%, and the Candida lipolytica cell body is increased by 2-3%. The mixed feed yeast is obtained by fermentation, which reduces the pressure of sewage treatment and realizes resource utilization. DETAILED DESCRIPTION

[0021] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0022] Saccharomyces cerevisiae was purchased from Angel Yeast Co., Ltd.

[0023] The sources of the erythritol ceramic membrane concentrated phase and the erythritol nanofiltration membrane concentrated phase can be found in Patent 202411366460.6. After treatment with the ceramic membrane, the erythritol ceramic membrane concentrated phase is retained; after treatment with the nanofiltration membrane, the erythritol nanofiltration membrane concentrated phase is retained.

[0024] Example 1

[0025] A method for producing yeast for feed using erythritol concentrated phase comprises the following steps:

[0026] (1) Preparation of erythritol concentrated phase: The erythritol ceramic membrane concentrated phase obtained by fermentation of Candida lipolytica (soluble solid content of 10.0%, pH of 3.9, conductivity of 2.0 ms / cm, reducing sugar content of 10.0% of the soluble solid content) and the erythritol nanofiltration membrane concentrated phase (soluble solid content of 20.0%, pH of 3.9, conductivity of 3.0 ms / cm; reducing sugar content of 60.0% of the soluble solid content) were mixed in a volume ratio of 1:1 to obtain an erythritol concentrated phase mixture;

[0027] (2) Saccharomyces cerevisiae fermentation: The erythritol concentrated phase mixture was adjusted to pH 5.0 with 4% sodium hydroxide solution, and inoculated with Saccharomyces cerevisiae with an initial OD value of 15 for fermentation, with an inoculation amount of 1.0%; the fermentation conditions were controlled as follows: temperature 29°C, stirring speed 30 r / min, dissolved oxygen 5%; after fermentation for 30 h, the residual sugar content was detected by titration to be less than 0.2%, and the fermentation liquid was removed from the tank to obtain the fermentation liquid;

[0028] (3) Ceramic membrane filtration: The fermentation liquid in the lower tank is filtered through a ceramic membrane with a pore size of 0.1 μm to obtain a new ceramic membrane concentrated phase; pure water is added to the new ceramic membrane concentrated phase for washing. When the soluble solids content of the ceramic membrane permeate is ≤0.5, washing is stopped to obtain a mixture of Candida lipolytica and Saccharomyces cerevisiae;

[0029] (4) Drum Drying and Pulverization: The mixture of Candida lipolytica and Saccharomyces cerevisiae was dried in a drum dryer at a drying temperature of 110°C. The dried yeast cells were pulverized in a pulverizer to obtain dried feed yeast. The dried feed yeast contained 8.0% Saccharomyces cerevisiae cells and 7.0% Candida lipolytica cells.

[0030] Control group (direct drying of the erythritol ceramic membrane concentrated phase in step (1)): Saccharomyces cerevisiae cell content = 0%, Candida lipolytica cell content = 5.0%.

[0031] Compared with the control group, Example 1 increased the number of Saccharomyces cerevisiae cells by 8% and the number of Candida lipolytica cells by 2%.

[0032] (5) The feed yeast obtained in Example 1 was added to animal feed: 10 kg / ton was added to livestock feed, 40 kg / ton was added to poultry feed, and 40 kg / ton was added to fish and shrimp feed. The results showed that compared with the control group (commercially available animal feed), the livestock feeding rate increased by 5%, and the weight gain rate increased by 6%; the poultry feeding rate increased by 5.5%, and the weight gain rate increased by 6.6%; the fish and shrimp feeding rate increased by 5.6%, and the weight gain rate increased by 7%.

[0033] Example 2

[0034] A method for producing yeast for feed using erythritol concentrated phase comprises the following steps:

[0035] (1) Preparation of erythritol concentrated phase: The erythritol ceramic membrane concentrated phase obtained by fermentation of Candida lipolytica (soluble solid content of 12.0%, pH of 4.0, conductivity of 2.5 ms / cm, reducing sugar content of 12.5% ​​of soluble solid content) and the erythritol nanofiltration membrane concentrated phase (soluble solid content of 23.0%, pH of 4.0, conductivity of 4.0 ms / cm; reducing sugar content of 65.0% of soluble solid content) were mixed in a volume ratio of 1:1.5 to obtain an erythritol concentrated phase mixture;

[0036] (2) Saccharomyces cerevisiae fermentation: The erythritol concentrated phase mixture was adjusted to pH 5.3 with 4% sodium hydroxide solution, and inoculated with Saccharomyces cerevisiae with an initial OD value of 20 for fermentation at an inoculation rate of 1.3%. The fermentation conditions were controlled as follows: temperature 30°C, stirring speed 40 r / min, dissolved oxygen 7%. After fermentation for 33 h, the residual sugar content was detected by titration to be less than 0.2%, and the fermentation liquid was removed from the tank to obtain the fermentation liquid.

[0037] (3) Ceramic membrane filtration: The fermentation liquid in the lower tank is filtered through a ceramic membrane with a pore size of 0.1 μm to obtain a new ceramic membrane concentrated phase; pure water is added to the new ceramic membrane concentrated phase for washing. When the soluble solids content of the ceramic membrane permeate is ≤0.5, washing is stopped to obtain a mixture of Candida lipolytica and Saccharomyces cerevisiae;

[0038] (4) Drum Drying and Pulverization: The mixture of Candida lipolytica and Saccharomyces cerevisiae was dried in a drum dryer at a drying temperature of 113°C. The dried yeast cells were pulverized in a pulverizer to obtain dried feed yeast. The dried feed yeast contained 9.0% Saccharomyces cerevisiae cells and 8.0% Candida lipolytica cells.

[0039] Control group (direct drying of the erythritol ceramic membrane concentrated phase in step (1)): Saccharomyces cerevisiae bacterial content = 0%, Candida lipolytica bacterial content = 5.5%.

[0040] Compared with the control group, Example 2 increased the number of Saccharomyces cerevisiae cells by 9% and the number of Candida lipolytica cells by 2.5%.

[0041] (5) The feed yeast obtained in Example 2 was added to animal feed: 13 kg / ton was added to livestock feed, 45 kg / ton was added to poultry feed, and 45 kg / ton was added to fish and shrimp feed. The results showed that compared with the control group (commercially available animal feed), the livestock feeding rate increased by 5.5%, and the weight gain rate increased by 6.6%; the poultry feeding rate increased by 6.0%, and the weight gain rate increased by 6.8%; the fish and shrimp feeding rate increased by 6.0%, and the weight gain rate increased by 7.3%.

[0042] Example 3

[0043] A method for producing yeast for feed using erythritol concentrated phase comprises the following steps:

[0044] (1) Preparation of erythritol concentrated phase: The erythritol ceramic membrane concentrated phase obtained by fermentation of Candida lipolytica (soluble solid content of 13.0%, pH of 4.1, conductivity of 3.0 ms / cm, reducing sugars accounting for 15.0% of the soluble solid content) and the erythritol nanofiltration membrane concentrated phase (soluble solid content of 25.0%, pH of 4.1, conductivity of 5.0 ms / cm; reducing sugars accounting for 70.0% of the soluble solid content) were mixed in a volume ratio of 1:2 to obtain an erythritol concentrated phase mixture;

[0045] (2) Saccharomyces cerevisiae fermentation: The erythritol concentrated phase mixture was adjusted to pH 5.5 with 4% sodium hydroxide solution, and inoculated with Saccharomyces cerevisiae with an initial OD value of 25 for fermentation at an inoculation rate of 1.5%. The fermentation conditions were controlled as follows: temperature 31°C, stirring speed 50 r / min, and dissolved oxygen 10%. After fermentation for 36 h, the residual sugar content was detected by titration to be less than 0.2%, and the fermentation liquid was removed from the tank to obtain the fermentation liquid.

[0046] (3) Ceramic membrane filtration: The fermentation liquid in the lower tank is filtered through a ceramic membrane with a pore size of 0.1 μm to obtain a new ceramic membrane concentrated phase; pure water is added to the new ceramic membrane concentrated phase for washing. When the soluble solids content of the ceramic membrane permeate is ≤0.5, washing is stopped to obtain a mixture of Candida lipolytica and Saccharomyces cerevisiae;

[0047] (4) Drum Drying and Pulverization: The mixture of Candida lipolytica and Saccharomyces cerevisiae was dried in a drum dryer at a drying temperature of 115°C. The dried yeast cells were pulverized in a pulverizer to obtain dried feed yeast. The dried feed yeast contained 10.0% Saccharomyces cerevisiae cells and 9.0% Candida lipolytica cells.

[0048] Control group (direct drying of the erythritol ceramic membrane concentrated phase in step (1)): Saccharomyces cerevisiae bacterial content = 0%, Candida lipolytica bacterial content = 6.0%.

[0049] Compared with the control group, Example 3 increased the number of Saccharomyces cerevisiae cells by 10% and the number of Candida lipolytica cells by 3.0%.

[0050] (5) The feed yeast obtained in Example 3 was added to animal feed: 15 kg / ton was added to livestock feed, 50 kg / ton was added to poultry feed, and 50 kg / ton was added to fish and shrimp feed. The results showed that compared with the control group (commercially available animal feed), the livestock feeding rate increased by 5.4%, and the weight gain rate increased by 6.3%; the poultry feeding rate increased by 5.8%, and the weight gain rate increased by 6.5%; the fish and shrimp feeding rate increased by 5.5%, and the weight gain rate increased by 7.0%.

[0051] Experimental data description:

[0052] Experimental group: Feed yeast powder was added to the feed according to the addition ratio of Examples 1-3;

[0053] Control group: fed with commercially available animal feed (beef cattle: Calf Kang cattle feed; broiler chickens: Changwang brand chicken feed; carp: Kaikou Huanyu feed; all purchased from Shandong New Hope Liuhe Group Co., Ltd.);

[0054] Sample size: 10 beef cattle (weighing 100-150 kg, similar total weight) were selected for each livestock group; 30 broiler chickens (weighing 1-1.5 kg, similar total weight) were selected for each poultry group; 50 carp (weighing 400-500 g, similar total weight) were selected for each fish and shrimp group;

[0055] Experimental method: The average 30-day food intake and 30-day weight gain of the experimental and control groups were statistically analyzed; the results of food intake rate and weight gain rate are shown in Table 1.

[0056] Table 1

[0057]

[0058]

[0059] Feeding rate = (average feeding amount of experimental group - average feeding amount of control group) / average feeding amount of control group * 100%;

[0060] Weight gain rate = (average weight gain of experimental group - average weight gain of control group) / average weight gain of control group * 100%.

[0061] As can be seen from Table 1, the feeding rate of livestock animals in Examples 1-3 of the present invention increased by 5.0-5.5%, and the weight gain rate was 6.0-6.6%; the feeding rate of poultry animals increased by 5.5-6.0%, and the weight gain rate increased by 6.5-6.8%; the feeding rate of fish and shrimp animals increased by 5.5-6.0%, and the weight gain rate increased by 7.0-7.3%.

[0062] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for producing yeast for feed using erythritol concentrated phase, characterized in that: The following steps are involved: (1) Preparation of erythritol concentrated phase: The erythritol ceramic membrane concentrated phase obtained by fermentation of Candida lipolytica and the erythritol nanofiltration membrane concentrated phase were mixed in a volume ratio of 1:1-2 to obtain an erythritol concentrated phase mixture; (2) fermentation with brewer's yeast: the erythritol concentrated phase mixture is adjusted to a pH of 5.0-5.5 with a 4% sodium hydroxide solution, and the fermentation is carried out by inoculating brewer's yeast with an initial OD value of 15-25, with an inoculation amount of 1.0-1.5%; the fermentation conditions are controlled as follows: temperature 29-31°C, stirring speed 30-50 r / min, dissolved oxygen 5-10%; fermentation is carried out for 30-36 hours, and the residual sugar is detected by titration method to be less than 0.2%, and the fermentation liquid is removed from the tank to obtain the fermentation liquid; (3) Ceramic membrane filtration: The fermentation liquid in the lower tank is filtered through a ceramic membrane to obtain a new ceramic membrane concentrated phase; pure water is added to the new ceramic membrane concentrated phase for washing, and the washing is stopped when the soluble solid content of the ceramic membrane permeate is ≤0.5 to obtain a mixture of Candida lipolytica and Saccharomyces cerevisiae; (4) Drum drying and crushing: The mixture of Candida lipolytica and Saccharomyces cerevisiae is dried using a drum dryer, and the dried yeast bodies are crushed using a crusher to obtain dry feed yeast.

2. The method for producing feed yeast using erythritol concentrated phase according to claim 1, characterized in that: The soluble solid content in the concentrated phase of the erythritol ceramic membrane in step (1) is 10.0-13.0%, the pH is 3.9-4.1, and the conductivity is 2.0-3.0 ms / cm, wherein reducing sugar accounts for 10.0-15.0% of the soluble solid content; The soluble solid content in the concentrated phase of the erythritol nanofiltration membrane is 20.0-25.0%, the pH is 3.9-4.1, and the conductivity is 3.0-5.0 ms / cm; and the reducing sugar accounts for 60.0-70.0% of the soluble solid content.

3. The method for producing feed yeast using erythritol concentrated phase according to claim 1, characterized in that: The pore size of the ceramic membrane in step (3) is 0.1 μm.

4. The method for producing feed yeast using erythritol concentrated phase according to claim 1, characterized in that: The drying temperature of the drum dryer in step (4) is 110-115°C.

5. The method for producing feed yeast using erythritol concentrated phase according to claim 1, characterized in that: The obtained feed yeast is added to animal feed in proportion. The proportion of feed yeast added to animal feed is 10-15 kg / ton for livestock feed, 40-50 kg / ton for poultry feed, and 40-50 kg / ton for fish and shrimp feed.

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