Anti-fatigue gastrodia elata and ginseng teabag as well as preparation method and application thereof

Abstract

The invention discloses an anti-fatigue gastrodia elata and ginseng teabag as well as a preparation method and application thereof, and belongs to the technical field of functional food processing. The tea bag comprises the following components in percentage by mass: 30%-50% of gastrodia elata extract, 20%-30% of ginseng extract, 10%-20% of auxiliary herbal components and 12%-30% of carrier auxiliary materials, and is packaged by a degradable filter bag. Wherein the gastrodia elata extract is prepared through a composite enzymolysis-ultrasonic-assisted extraction method, the ginseng extract is prepared through extraction by adopting a low-temperature ultrasonic-ethanol precipitation combined process, and the two active ingredients are in synergistic interaction according to the mass ratio of the gastrodia elata extract to the ginseng extract being 1: (1.5-2). The freeze-drying process is adopted to retain effective components, and the degradable corn fiber / PLA filter bag is used for packaging, so that the stability and environmental protection property of the product are ensured. The traditional Chinese medicine is suitable for people with long-term mental or physical fatigue, and 1-2 bags of the traditional Chinese medicine can effectively reduce blood lactic acid accumulation every day.

Description

Technical Field

[0001] The invention relates to the technical field of functional food processing, in particular to an anti-fatigue gastrodia elata and ginseng tea bag, and a preparation method and application thereof. Background Art

[0002] As the pace of modern life accelerates, fatigue is becoming increasingly serious. Long-term mental or physical exertion can easily lead to decreased liver glycogen reserves, accumulation of blood lactate, and increased oxidative stress. Traditional anti-fatigue products often rely on a single active ingredient (such as caffeine or ginsenosides), resulting in short-lived effects and high irritation. Gastrodin and ginsenosides, as natural anti-fatigue active substances, have the potential to regulate the central nervous system and improve energy metabolism. However, existing tea bag products lack scientific extraction processes and formulation design, resulting in low dissolution rates of the active ingredients (gastrodin <0.8 mg / g, ginsenoside Rg1 + ginsenoside Re <2.0 mg / g), and a lack of synergistic mechanisms, preventing them from fully realizing their anti-fatigue effects.

[0003] Currently, commercially available tea bags typically utilize simple hot water immersion or mechanical crushing processes. This process, however, results in gastrodin decomposing easily at high temperatures, while ginsenosides exhibit insufficient solubility in pure water extraction. Furthermore, conventional packaging materials (such as nylon filter bags) can lead to oxidation or migration of the active ingredient. In related technologies, ethanol extraction of gastrodin exhibits poor stability. Currently, process parameter optimization for the synergistic anti-fatigue effects of gastrodia elata and ginseng has not been conducted, and the optimal gastrodia elata-ginseng compound ratio remains under investigation. Therefore, it is crucial to develop a technical solution for anti-fatigue tea bags that combines efficient extraction with stable preparation. Summary of the Invention

[0004] In order to solve the problem that existing tea bag products lack scientific extraction technology and ratio design, the present invention provides an anti-fatigue gastrodia elata and ginseng tea bag. The tea bag is composed of gastrodia elata extract, ginseng extract, auxiliary herbal ingredients, and carrier excipients. The mass percentage of each component is: gastrodia elata extract 17%-28% (gastrodin content ≥1.2 mg / g), ginseng extract 33%-47% (ginsenoside Rg1+Re content ≥2.5 mg / g), auxiliary herbal ingredients 10%-20% (one or more of liquorice, polygonatum, and wolfberry), and carrier excipients 12%-30% (microcrystalline cellulose or maltodextrin); the tea bag is packaged in a degradable filter bag.

[0005] The Gastrodia elata extract of the present invention is extracted by composite enzymatic hydrolysis-ultrasound-assisted extraction.

[0006] The ginseng extract of the invention is extracted by a low-temperature ultrasound-ethanol precipitation combined method.

[0007] Preferably, the auxiliary herbs of the present invention are one or more of liquorice (to enhance sweetness), polygonatum (to replenish qi), and wolfberry (to resist oxidation).

[0008] Preferably, the mass ratio of the Gastrodia elata extract to the ginseng extract is 1:1.5-2. The Gastrodia elata extract and the ginseng extract can activate the AMPK-α pathway and promote the synthesis of liver glycogen.

[0009] The preparation method of the anti-fatigue gastrodia elata and ginseng tea bag of the present invention is as follows: (1) Preparation of Gastrodia elata extract, specifically comprising: adding 5-10 times the mass of water to 60-80 mesh Gastrodia elata powder; adding cellulase and pectinase, with the total enzyme activity of the complex enzyme ≥500U / g, and performing enzymatic hydrolysis at 50-55°C for 1-1.5h; ultrasonic-assisted extraction, with the ultrasonic conditions of frequency 40kHz, power 400-600W, temperature 70-80°C, and time 30-50min, filtering to obtain an extract; concentrating the extract at a temperature of 50-60°C and a vacuum degree of -0.08MPa to a solid content of ≥20%; and freeze-drying at a temperature of -50°C and a pressure of 10Pa to obtain a freeze-dried powder of Gastrodia elata extract.

[0010] (2) Preparation of ginseng extract, specifically including: mixing 40-60 mesh ginseng powder with 40%-60% ethanol in a volume ratio of 1:8-1:10, ultrasonic extraction at 60-70°C with a frequency of 40 kHz for 30-40 min, and filtering; after the filtrate is concentrated under reduced pressure, it is allowed to stand at 4°C for 12 h, centrifuged at a speed of 8000 rpm for 15 min, and the supernatant is collected; the inlet air temperature is 160°C, and the outlet air temperature is 80°C, and the ginseng extract powder is obtained by spray drying.

[0011] (3) Preparation of degradable filter bags, specifically including: melt spinning of corn fiber or PLA to make non-woven fabric with a pore size of 50-100 μm; ultrasonic heat sealing (temperature 120-130°C, pressure 0.2 MPa), wherein the temperature resistance of the degradable filter bag is ≥120°C.

[0012] (4) Mix the above components in proportion to ensure uniformity (RSD < 3%); fill into PLA filter bags (2.0 ± 0.1 g / bag), and ultrasonically heat seal the edges (120 ° C, pressure 0.2 MPa) to obtain anti-fatigue gastrodia elata and ginseng tea bags.

[0013] Beneficial effects of the present invention: The anti-fatigue Gastrodia elata and ginseng tea bag of the present invention is based on a specific ratio design and a freeze-drying process to prepare a tea bag with synergistic anti-fatigue effect, filling the technical gap in the field of Gastrodia elata-ginseng synergistic anti-fatigue tea bags. The present invention uses a degradable filter bag, which is energy-saving and environmentally friendly and can also avoid oxidation or migration of active ingredients.

[0014] Animal experiments have shown that the tea bag can significantly prolong the weight-bearing swimming time of mice (≥80%), increase liver glycogen reserves (≥30%), and enhance the DPPH free radical scavenging ability (≥85%). The present invention can improve the physical function of people with long-term mental or physical fatigue. Drinking 1 to 2 bags (2g per bag) daily for 14 consecutive days can significantly reduce the accumulation of blood lactate after exercise ( p <0.05). BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The present invention is a schematic diagram of the tea bag preparation process. DETAILED DESCRIPTION

[0016] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, but the protection scope of the present invention is not limited to the contents described above.

[0017] Example 1 The specific ingredients and contents of the anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment are shown in Table 1.

[0018] Table 1 The anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment is the best anti-fatigue ratio, and the specific preparation process is as follows: (1) The preparation method of Gastrodia elata extract is as follows: Enzymatic hydrolysis: Gastrodia elata powder (60 mesh) was mixed with 5 times the amount of water, and cellulase (400 U / g) and pectinase (250 U / g) were added, pH 5.0, and the reaction was carried out at 52°C for 1.2 h.

[0019] Ultrasonic extraction: ultrasonication at 40 kHz and 550 W power at 75 °C for 45 min, and the extract was filtered.

[0020] Concentration and drying: Concentrate under reduced pressure (-0.08 MPa, 60°C) to a solid content of 22%, and freeze-dry (pre-freeze at -50°C for 2 h, then gradient sublimation at -20°C → 25°C for 6 h).

[0021] (2) The preparation method of ginseng extract is as follows: Ultrasonic extraction: Ginseng powder (50 mesh) and 50% ethanol (1:9 w / v) were ultrasonically extracted at 60°C for 35 min.

[0022] Purification: After concentration, let it stand at 4°C for 12 hours, centrifuge (8000 rpm, 20 minutes) and collect the supernatant.

[0023] Spray drying: air inlet temperature 165℃, air outlet temperature 85℃, to obtain powder (moisture ≤ 4%).

[0024] (3) Mix the components according to the proportions in Table 1 to ensure uniformity (RSD < 3%); fill into PLA filter bags (2.0 ± 0.1 g / bag), and ultrasonically heat seal the edges (120 ° C, 0.2 MPa) to obtain anti-fatigue Gastrodia elata and ginseng tea bags.

[0025] In order to further verify the function of the anti-fatigue Gastrodia elata and ginseng teabag prepared in this example, an animal experiment was conducted. The specific experimental process is as follows: Thirty healthy male Kunming mice (SPF grade, weighing 18–22 g) were selected and acclimated for 7 days before the experiment. The mice were randomly divided into three groups, each with 10 mice: a normal control group, a fatigue model group (model control group), and a tea bag intervention group.

[0026] Administration method: The normal control group and the model control group were given normal saline (40 mg / kg) by gavage every day; the tea bag group was given tea bag extract (150 mg / kg) by gavage every day.

[0027] All mice were gavage-administered once a day at 9:00 am for 14 consecutive days.

[0028] Fatigue model establishment and index detection: One hour after the last gavage on day 14, mice were subjected to a weighted swimming test (a weight equivalent to 5% of body weight was placed on the tail). The time to exhaustion was recorded to assess fatigue resistance. Blood was then quickly collected via orbital blood collection, and serum was separated for biochemical analysis. Blood lactate level (post-exercise): Blood was collected via eyeball blood collection after exercise to measure blood lactate levels. Finally, the mice were sacrificed, and liver tissue was homogenized and assayed for glycogen content.

[0029] The results of the animal experiments are shown in Table 2.

[0030] Table 2 Table 2 shows that the anti-fatigue Gastrodia elata and ginseng teabag prepared in Example 1 exhibits significant anti-fatigue and antioxidant effects. Compared to the normal control group, the mice in the teabag group in Example 1 experienced an 85% increase in swimming time, a 55% increase in blood lactate clearance, an 88% increase in DPPH free radical clearance, and a 32% increase in liver glycogen, demonstrating its outstanding performance in enhancing exercise endurance, alleviating fatigue, and scavenging oxidative free radicals. This superior effect is primarily due to the synergistic effect of the ingredients in the formula: the Gastrodia elata extract (40%) is rich in gastrodin, which has neuromodulatory and anti-fatigue properties; the ginseng extract (30%) is rich in ginsenosides Rg1 and Re, which promote energy metabolism and enhance exercise adaptability. The combination of these two ingredients produces a significant synergistic effect. Polygonatum sibiricum powder (15%) is rich in polysaccharides, which tonifies Qi, strengthens the body, and enhances immunity, further enhancing physical recovery. Maltodextrin (15%), as a carrier excipient, facilitates the slow release of energy and improves ingredient stability, maintaining a sustained anti-fatigue effect. In addition, freeze-drying and ultrasonic extraction processes are used to retain the biological activity of the active ingredients to the maximum extent, ensuring the efficient release and action of various functional substances, thereby giving this formula comprehensive and sustained anti-fatigue capabilities.

[0031] Example 2 The specific ingredients and contents of the anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment are shown in Table 3.

[0032] Table 3 The anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment has a high gastrodin ratio, and the specific preparation process is as follows: (1) The preparation method of Gastrodia elata extract is as follows: Enzymatic hydrolysis: Gastrodia elata powder (60 mesh) was mixed with 5 times the amount of water, and cellulase (400 U / g) and pectinase (250 U / g) were added. The enzymatic hydrolysis pH was 4.8 and the reaction was carried out at 52°C for 1.2 h.

[0033] Ultrasonic extraction: ultrasonication at 40 kHz and 600 W power at 75 °C for 45 min, and the extract was filtered.

[0034] Concentration and drying: Concentrate under reduced pressure (-0.08 MPa, 50°C) to a solid content of 22%, and freeze-dry (pre-freeze at -50°C for 2 h, then gradient sublimation at -20°C → 25°C for 6 h).

[0035] (2) The preparation method of ginseng extract is as follows: Ultrasonic extraction: Ginseng powder (50 mesh) and 60% ethanol (1:8 w / v) were ultrasonically extracted at 65°C for 40 min.

[0036] Purification: After concentration, let it stand at 4°C for 12 hours, centrifuge (8000 rpm, 20 minutes) and collect the supernatant.

[0037] Spray drying: air inlet temperature 165℃, air outlet temperature 85℃, to obtain powder (moisture ≤ 4%).

[0038] (3) Mix the components according to the proportions in Table 3 to ensure uniformity (RSD < 3%); fill into corn fiber filter bags (2.0 ± 0.1 g / bag), and ultrasonically heat seal the edges (120 ° C, 0.2 MPa) to obtain anti-fatigue gastrodia elata and ginseng tea bags.

[0039] In order to further verify the anti-fatigue function of the Gastrodia elata and ginseng teabag prepared in this example, an animal experiment was conducted. The animal experiment was the same as that in Example 1. The results are shown in Table 4.

[0040] Table 4 Table 4 shows that the anti-fatigue Gastrodia elata and ginseng teabag from Example 2 demonstrated significant anti-fatigue effects in mice. Specifically, this was demonstrated by a 78% increase in swimming time, a 50% blood lactate clearance rate, and significant neuroprotective effects (65% inhibition of acetylcholinesterase). These results demonstrate that this formula offers significant advantages in improving exercise endurance, accelerating lactate clearance, and providing neuroprotection. This can be attributed to the synergistic effects of the various ingredients. First, the Gastrodia elata extract (50%) contains a high content of gastrodin (≥1.8 mg / g), which helps alleviate fatigue, improve neurological function, and enhance physical recovery. Second, the ginseng extract (20%) is rich in ginsenosides Rg1 and Re, which can enhance energy metabolism, boost physical strength, and alleviate fatigue. The combination of the two creates a favorable synergistic effect. The addition of wolfberry powder (10%), a supplementary ingredient rich in flavonoids with antioxidant and neuroprotective properties, further enhances the overall anti-fatigue and neuroprotective effects. Maltodextrin (10%), as a carrier excipient, provides a stable physical form and sustained energy support, ensuring a continuous supply of physical strength. Furthermore, microcrystalline cellulose (10%) helps stabilize tea particles and supports sustained energy release, promoting sustained physical output. Through precise ingredient ratios and an optimized preparation process (freeze-drying and ultrasonic extraction), this tea bag effectively retains the potency of each active ingredient, achieving comprehensive anti-fatigue and neuroprotective effects.

[0041] Example 3 The specific ingredients and contents of the anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment are shown in Table 5.

[0042] Table 5 The anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment is a low-cost formulation, and the specific preparation process is as follows: (1) The preparation method of Gastrodia elata extract is as follows: Enzymatic hydrolysis: Gastrodia elata powder (60 mesh) was mixed with 5 times the amount of water, and cellulase (400 U / g) and pectinase (250 U / g) were added. The enzymatic hydrolysis pH was 4.8 and the reaction was carried out at 50°C for 1 h.

[0043] Ultrasonic extraction: ultrasonically extract at 40 kHz and 400 W power at 75 °C for 45 min, and filter to obtain the extract.

[0044] Concentration and drying: Concentrate under reduced pressure (-0.08 MPa, 55°C) to a solid content of 22%, and freeze-dry (pre-freeze at -50°C for 2 h, then gradient sublimation at -20°C → 25°C for 6 h).

[0045] (2) The preparation method of ginseng extract is as follows: Ultrasonic extraction: Ginseng powder (50 mesh) and 40% ethanol (1:10 w / v) were ultrasonically extracted at 65°C for 40 min.

[0046] Purification: After concentration, centrifuge (8000 rpm, 20 min) and take the supernatant.

[0047] Spray drying: air inlet temperature 165℃, air outlet temperature 85℃, to obtain powder (moisture ≤ 4%).

[0048] (3) Mix the components according to the proportions in Table 3 to ensure uniformity (RSD < 3%); fill into corn fiber filter bags (2.0 ± 0.1 g / bag), and ultrasonically heat seal the edges (120 ° C, 0.2 MPa) to obtain anti-fatigue gastrodia elata and ginseng tea bags.

[0049] In this embodiment, the enzymatic hydrolysis time for Gastrodia elata extraction is shortened to 45 minutes, and the total cost is reduced by 20%; the ethanol concentration for ginseng extraction is reduced to 40%, and the purification step does not require standing at 4°C for 12 hours, reducing the time consumption by 50%.

[0050] In order to further verify the anti-fatigue function of the Gastrodia elata and ginseng teabag prepared in this example, an animal experiment was conducted. The animal experiment was the same as that in Example 1. The results are shown in Table 6.

[0051] Table 6 Table 6 shows that the anti-fatigue Gastrodia elata and ginseng teabag from Example 3 demonstrated significant anti-fatigue effects in mice, demonstrated by a 70% increase in swimming time and a 25% increase in liver glycogen. These results indicate that the formula exhibits a moderate anti-fatigue effect, improving physical strength and boosting energy reserves. This can be attributed to the synergistic effects of the ingredients in the formula. The Gastrodia elata extract (30%) is rich in gastrodin, which regulates the central nervous system, relieves fatigue, and improves physical strength. The ginsenosides Rg1 and Re in the ginseng extract (20%) help enhance energy metabolism and improve exercise endurance. The combination of these two ingredients exerts a potent anti-fatigue effect. Furthermore, licorice powder (20%), as an auxiliary ingredient, contains glycyrrhizic acid, which modulates the immune system and enhances antioxidant capacity, further enhancing the overall anti-fatigue effect. Maltodextrin (30%), as a carrier excipient, provides a stable physical form and supports the slow release of energy, ensuring a sustained supply of energy. Optimized extraction processes (such as shortening the Gastrodia elata extraction and enzymatic hydrolysis time and adjusting the ethanol concentration) not only ensure efficient extraction of the ingredients but also reduce costs. Therefore, the tea bag formula shows better effects in anti-fatigue, especially in energy replenishment and fatigue recovery.

[0052] Example 4 The specific ingredients and contents of the anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment are shown in Table 7.

[0053] Table 7 The anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment is an antioxidant-enhanced formula, in which vitamin C and polyphenol components synergistically enhance antioxidant capacity. The specific preparation process is as follows: (1) The preparation method of Gastrodia elata extract is as follows: Enzymatic hydrolysis: Gastrodia elata powder (60 mesh) was mixed with 5 times the amount of water, and cellulase (400 U / g) and pectinase (250 U / g) were added. The enzymatic hydrolysis pH was 4.8 and the reaction was carried out at 55°C for 1 h.

[0054] Ultrasonic extraction: ultrasonically extract at 40 kHz and 600 W power at 70 °C for 50 min, and filter to obtain the extract.

[0055] Concentration and drying: Concentrate under reduced pressure (-0.08 MPa, 60°C) to a solid content of 22%, and freeze-dry (pre-freeze at -50°C for 2 h, then gradient sublimation at -20°C → 25°C for 6 h).

[0056] (2) The preparation method of ginseng extract is as follows: Ultrasonic extraction: Ginseng powder (50 mesh) and 40% ethanol (1:9 w / v) were ultrasonically extracted at 65°C for 40 min.

[0057] Purification: After concentration, centrifuge (8000 rpm, 20 min) and take the supernatant.

[0058] Spray drying: air inlet temperature 165℃, air outlet temperature 85℃, to obtain powder (moisture ≤ 4%).

[0059] (3) Mix the components according to the proportions in Table 3 to ensure uniformity (RSD < 3%); fill into PLA filter bags (2.0 ± 0.1 g / bag), and seal the edges with ultrasonic heat sealing (120 ° C, 0.2 MPa) to obtain anti-fatigue Gastrodia elata and ginseng tea bags.

[0060] In order to further verify the anti-fatigue function of the Gastrodia elata and ginseng teabag prepared in this example, an animal experiment was conducted. The animal experiment was the same as that in Example 1. The results are shown in Table 8.

[0061] Table 8 Table 8 shows that the anti-fatigue Gastrodia elata and ginseng teabag from Example 4 demonstrated significant anti-fatigue effects in mice, demonstrated by a DPPH scavenging rate of 92% and an 80% increase in swimming time. These results demonstrate the potent antioxidant and anti-fatigue efficacy of this formula. This can be attributed to the synergistic effects of the various ingredients. First, the Gastrodia elata extract (35%), with a gastrodin content of ≥1.3 mg / g, effectively regulates the central nervous system, relieves fatigue, and enhances physical strength. The ginsenosides Rg1 and Re in the ginseng extract (25%) enhance physical strength and improve energy metabolism. The combination of these two enhances the overall anti-fatigue effect. Maltodextrin (20%), as a carrier excipient, provides sustained energy support. Lycium barbarum and grape seed powder (15%), as antioxidant ingredients rich in proanthocyanidins and polyphenols, effectively scavenge free radicals, mitigate oxidative stress, and further enhance the anti-fatigue and neuroprotective effects. Vitamin C (5%) works synergistically with the polyphenols to significantly boost the overall antioxidant capacity, helping to protect cells from damage. The interaction of these ingredients not only enhances the antioxidant effect, but also increases exercise endurance and reduces fatigue. Through precise ingredient ratios and optimized extraction processes, we ensure that each active ingredient is maximized, thus achieving significant anti-fatigue and antioxidant effects.

[0062] Example 5 The specific ingredients and contents of the anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment are shown in Table 9.

[0063] Table 9 The anti-fatigue Gastrodia elata and ginseng teabag described in this embodiment is an absorption-promoting formula, and the pore size of the filter bag is reduced to 50 μm, so the sustained-release effect is better. The specific preparation process is as follows: (1) The preparation method of Gastrodia elata extract is as follows: Enzymatic hydrolysis: Gastrodia elata powder (60 mesh) was mixed with 5 times the amount of water, and cellulase (400 U / g) and pectinase (250 U / g) were added. The enzymatic hydrolysis pH was 4.8 and the reaction was carried out at 50°C for 1.5 h.

[0064] Ultrasonic extraction: ultrasonically extract at 40 kHz and 600 W power at 80 °C for 30 min, and filter to obtain the extract.

[0065] Concentration and drying: Concentrate under reduced pressure (-0.08 MPa, 60°C) to a solid content of 22%, and freeze-dry (pre-freeze at -50°C for 2 h, then gradient sublimation at -20°C → 25°C for 6 h).

[0066] (2) The preparation method of ginseng extract is as follows: Ultrasonic extraction: Ginseng powder (50 mesh) and 40% ethanol (1:9 w / v) were ultrasonically extracted at 70°C for 30 min.

[0067] Purification: After concentration, centrifuge (8000 rpm, 20 min) and take the supernatant.

[0068] Spray drying: air inlet temperature 165℃, air outlet temperature 85℃, to obtain powder (moisture ≤ 4%).

[0069] (3) Mix the components according to the proportions in Table 3 to ensure uniformity (RSD < 3%); fill into PLA filter bags (2.0 ± 0.1 g / bag), and seal the edges with ultrasonic heat sealing (120 ° C, 0.2 MPa) to obtain anti-fatigue Gastrodia elata and ginseng tea bags.

[0070] In order to further verify the anti-fatigue function of the Gastrodia elata and ginseng teabag prepared in this example, an animal experiment was conducted. The animal experiment was the same as that in Example 1. The results are shown in Table 10.

[0071] Table 10 Table 10 shows that the anti-fatigue Gastrodia elata and ginseng teabag in Example 5 demonstrates excellent bioavailability and sustained effect. Peak plasma concentration (Tmax) is advanced to 1.5 hours, and the anti-fatigue effect lasts for 6 hours, significantly outperforming conventional formulas. This effectiveness is primarily due to the synergistic effects of the ingredients and the optimized absorption mechanism. First, the Gastrodia elata extract (40%) is rich in gastrodin, which exhibits significant neuromodulatory and anti-fatigue effects. Ginsenosides Rg1 and Re in the ginseng extract (30%) improve energy metabolism and exercise endurance. Together, these two enhance the overall anti-fatigue properties of this formula. Polygonatum sibiricum powder (18%) is rich in polysaccharides, tonifying qi and blood, and boosting immunity. Maltodextrin (7%), as a carrier excipient, provides sustained energy support. The addition of phospholipids (5%) as an absorption-enhancing carrier excipient in this example effectively increases the bioavailability of gastrodin by approximately 30%, enabling faster and more complete absorption of the active ingredient, directly contributing to an earlier peak plasma concentration and a prolonged duration of action. The pore size of the filter bag is reduced to 50μm, which enhances the sustained release of ingredients and prevents the rapid loss of active substances. In summary, this formula, through scientific combination and technical optimization, has obvious advantages in improving absorption efficiency and prolonging the anti-fatigue effect.

[0072] Comparative Example 1 The components and specific operation steps of the Gastrodia elata and ginseng tea bag described in this comparative example 1 are as follows: Gastrodia elata (40%), ginseng (30%), wolfberry (15%), and maltodextrin (15%) were mixed in the mass percentages to ensure uniformity (RSD < 3%). The mixture was then filled into PLA filter bags (2.0 ± 0.1 g / bag) and sealed with ultrasonic heat sealing (120°C, 0.2 MPa) to produce Gastrodia elata and ginseng tea bags. Gastrodia elata and ginseng were mechanically ground into powders, with Gastrodia elata having a 60-80 mesh size and ginseng having a 40 mesh size.

[0073] In order to further verify the anti-fatigue function of the Gastrodia elata and ginseng teabag prepared in this comparative example, an animal experiment was conducted. The animal experiment was the same as that in Example 1. The results are shown in Table 11.

[0074] Table 11 As shown in Table 11, in Comparative Example 1, the mice's swimming time was extended by 35%, the blood lactate clearance rate was 25%, the DPPH free radical scavenging rate was 48%, and the liver glycogen increment was 15%. Its ability to enhance exercise endurance, relieve fatigue, and scavenge oxidative free radicals was significantly reduced. Compared with Example 1, Comparative Example 1 used a combined enzymatic hydrolysis-ultrasound-assisted extraction method for extracting Gastrodia elata extract and a low-temperature ultrasound-ethanol precipitation method for extracting ginseng extract. Mechanical pulverization results in a low dissolution rate of the active ingredients of Gastrodia elata and ginseng, significantly reducing the anti-fatigue effect of the tea bag.

[0075] Table 12 Table 12 is a comparative analysis table of anti-fatigue Gastrodia elata and ginseng tea bags prepared according to the proportions of the components in Examples 1 to 5. In Examples 1 to 5, the extension rate of mouse swimming time, blood lactate clearance rate, and liver glycogen increment were all improved, proving that the tea bags prepared in Examples 1 to 5 all had anti-fatigue effects; among them, the extension rate of mouse swimming time in Example 1 was the highest, because the content of the active ingredients gastrodin and ginsenoside Rg1+Re was the highest, and the anti-fatigue effect was the best.

[0076] While various embodiments of the present invention have been described above, the foregoing description is intended to be illustrative, non-exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is selected to best explain the principles of the embodiments, their practical applications, or improvements to existing technologies, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. An anti-fatigue gastrodia elata and ginseng tea bag, characterized in that: The tea bag raw materials are composed of gastrodia elata extract, ginseng extract, auxiliary herbal ingredients and carrier excipients. The mass percentage of each component is: gastrodia elata extract 17% to 28%, ginseng extract 33% to 47%, auxiliary herbal ingredients 10% to 20%, and carrier excipients 12% to 30%. The tea bag is packaged in a degradable filter bag.

2. The anti-fatigue gastrodia elata and ginseng tea bag according to claim 1, characterized in that: The degradable filter bag is made of corn fiber or PLA melt spinning, has a pore size of 50-100 μm, and a temperature resistance of 110° C.-130° C.

3. The anti-fatigue gastrodia elata and ginseng tea bag according to claim 1, characterized in that: The Gastrodia elata extract is prepared by a composite enzymatic hydrolysis-ultrasound-assisted extraction method, which specifically includes: The Gastrodia elata slices are crushed into 60-80 meshes, and 5-10 times the amount of water is added; cellulase and pectinase are added, the total enzyme activity of the complex enzyme is ≥500U / g, and enzymolysis is carried out at 50-55°C for 1-1.5 hours; ultrasonic-assisted extraction is carried out, the ultrasonic conditions are a frequency of 40kHz, a power of 400-600W, a temperature of 70-80°C, and a time of 30-50 minutes, and the extract is filtered to obtain the extract; the extract is concentrated at a temperature of 50-60°C and a vacuum degree of -0.08MPa to a solid content of ≥20%; and then freeze-dried at a temperature of -50°C and a pressure of 10Pa to obtain a Gastrodia elata extract freeze-dried powder.

4. The anti-fatigue gastrodia elata and ginseng tea bag according to claim 1, characterized in that: The ginseng extract is prepared by a low-temperature ultrasound-ethanol precipitation method, which specifically includes: The 40-60 mesh ginseng powder was mixed with 40%-60% ethanol in a volume ratio of 1:8-1:10, and ultrasonic extraction was performed at 60-70°C with a frequency of 40 kHz for 30-40 minutes, followed by filtration. The filtrate was concentrated under reduced pressure and allowed to stand at 4°C for 12 hours, and then centrifuged at a speed of 8000 rpm for 15 minutes to collect the supernatant. The ginseng extract powder was then spray-dried at an inlet air temperature of 160°C and an outlet air temperature of 80°C to obtain the ginseng extract powder.

5. The anti-fatigue gastrodia elata and ginseng tea bag according to claim 1, 3 or 4, characterized in that: Add Gastrodia elata extract and ginseng extract into tea bags at a mass ratio of 1:1.5~2.

6. Use of the anti-fatigue Gastrodia elata and ginseng tea bag according to claim 1, 2, 3 or 4 in anti-fatigue aspects.

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