Soothing and repairing composition and preparation method thereof

Abstract

The invention relates to the technical field of medicines and cosmetics, in particular to a soothing and repairing composition and a preparation method thereof, in the composition, a bionic liquid crystal system simulates a lamellar liquid crystal structure of skin intercellular lipid, the affinity and permeability between an external preparation and the skin surface are enhanced, and the delivery efficiency of active ingredients is remarkably improved. A stable moisturizing film is rapidly formed on the skin surface, percutaneous moisture loss is effectively reduced, the dry state is improved, and the epidermis water steady state is recovered. The plant bionic sebum is highly similar to human body sebum by extracting and reasonably reconstructing plant-derived fatty acid, cholesteryl ester and soothing components, and participates in the skin immune regulation and inflammation relieving process through multiple biological activity approaches such as anti-inflammation, anti-oxidation and soothing, so that active repairing and strengthening of a barrier function are realized, and the skin is protected. The skin barrier repairing and water locking functions are enhanced. The product is safe and mild, is suitable for various skin types, and has a good application prospect in the technical fields of medicines and cosmetics.

Description

Technical Field

[0001] The present invention relates to the technical field of medicine and cosmetics, in particular to a rapid soothing and repairing composition of a dual bionic liquid crystal system and a preparation method thereof. Background Art

[0002] As the human body's largest organ, the skin not only bears the critical responsibility of defending against external physical, chemical, and biological insults, but is also deeply involved in a series of complex physiological processes, including temperature regulation, sensory perception, and metabolism. The skin's barrier function is highly dependent on its unique tissue structure. The stratum corneum is composed of keratinocytes and intercellular lipids. Crucially, the intercellular lipids are arranged in an orderly liquid-crystal state, a property that plays an indispensable role in maintaining the skin's moisture homeostasis and barrier integrity.

[0003] In recent years, biomimetic liquid crystal systems have become a research hotspot due to their innovative advantages. By precisely mimicking the liquid crystal structure of intercellular lipids in the skin's stratum corneum, as well as the composition and function of the skin's natural moisturizing factor, these systems promise to achieve multiple benefits, including rapid onset of action, highly effective moisturization, deep repair of the skin barrier, and targeted, deep delivery of nutrients. Their unique liquid crystal structure significantly enhances the composition's affinity with the skin, thereby promoting the penetration and absorption of active ingredients, opening up new avenues and technological paths for the development of fast-acting, high-performance repair compositions. Simultaneously, the application of biomimetic technology in cosmetics is gradually evolving from single-structure biomimetic systems to multifunctional composite systems. Traditional barrier repair technologies mostly rely on exogenous component coverage (such as vaseline) or single bionic structures, but have problems such as slow response speed and insufficient dynamic adaptability. For example, CN114469843 A discloses a sebum biomimetic composition with soothing and repairing effects and its preparation method, which can achieve a repair and redness-reducing effect after 30 minutes of use; CN 112370364 A discloses a skin barrier repair composition containing a liquid crystal biomimetic emulsifier and its preparation method. After one month of use, the test subjects can effectively improve the redness and other discomfort symptoms after minimally invasive facial surgery.

[0004] As consumers' demands for sensitive skin care increase, there is an urgent need to develop innovative solutions that have both rapid response and long-term stabilization properties. Summary of the Invention

[0005] In response to the above problems, the present invention innovatively proposes a "bionic liquid crystal-plant sebum dual bionic system", that is, using a liquid crystal system and plant bionic sebum at the same time, combined with a soothing composition, to prepare a rapid soothing and repairing composition.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A soothing and repairing composition of a dual biomimetic liquid crystal system comprises the following components in percentage by weight: Component A: Dipropylene Glycol 1-3%, Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer 1-2%, Polysorbate 60 0.05-0.1%, Sorbitan Isostearate 0.05-0.1%, Glycerin 0.5-1.5%, Disodium EDTA 0.01-0.05%; Component B: biomimetic liquid crystal 1-5%, cyclopentasiloxane 1-5%, squalane 1-4%, ethylhexyl palmitate 1-2%, isononyl isononanoate 0.5-2%, plant biomimetic sebum 0.5-1.5%, dicaprylyl carbonate 0.1-0.9%; Component C: 0.1-1% p-hydroxyacetophenone, 0.1-1% 1,2-hexanediol; Component D: soothing composition 10-15%, butylene glycol 1-3%; Remaining water.

[0007] The biomimetic liquid crystal comprises 55-75% cetearyl alcohol, 10-20% phytosterols, 10-20% glyceryl isostearate, and 1-5% hydrogenated lecithin; The plant biomimetic sebum comprises 50-60% caprylic / capric triglyceride, 25-30% phytosterols, 10-20% glyceryl stearate, 0.1-1% tocopheryl acetate, 0.1-1% hydrogenated lecithin, 0.1-1% bisabolol, and 0.1-1% dipotassium glycyrrhizate; The soothing composition comprises: 40-50% of Aquilariae Lignum Resinatum extract, 5-15% of Juniper (Juniperus Communis) fruit extract, and 40-50% of sodium DNA.

[0008] In the soothing and repairing composition provided by this invention, the biomimetic liquid crystal system mimics the orderly arrangement of intercellular lipids in the skin, simulating the lamellar liquid crystal structure of intercellular lipids in the stratum corneum. This enhances the affinity and permeability of the topical formulation with the skin surface, significantly improving the delivery efficiency of active ingredients. Furthermore, the system possesses excellent water-binding capacity, rapidly forming a stable moisturizing film on the skin surface, effectively reducing transepidermal water loss (TEWL), improving dryness, and restoring epidermal water homeostasis. Secondly, the plant-based biomimetic sebum, through the extraction and reconstruction of plant-derived fatty acids, cholesterol esters, and a rational combination of soothing ingredients, achieves a high degree of similarity to human sebum, forming a dynamic biomimetic protective film on the surface. Through multiple bioactive pathways, including anti-inflammatory, antioxidant, and soothing effects, it participates in skin immune regulation and inflammation relief, thereby actively repairing and strengthening the skin's barrier function. These two synergistically promote the delivery of active ingredients, thereby enhancing the skin's barrier repair and moisture retention functions. The further combination of the soothing composition enables the composition to not only quickly soothe sensitive skin and fade redness within 15 minutes, but also has efficient moisturizing and barrier repair functions, promotes cell regeneration and wound healing, and provides deep nourishment for the skin, while ensuring the safety and mildness of the product, making it suitable for all skin types.

[0009] Preferably, the soothing and repairing composition comprises the following components in percentage by weight: Component A: Dipropylene Glycol 2%, Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer 1.5%, Polysorbate 60 0.08%, Sorbitan Isostearate 0.08%, Glycerin 1%, Disodium EDTA 0.03%; Component B: Bionic liquid crystal 3%, cyclopentasiloxane 3%, squalane 2%, ethylhexyl palmitate 1.5%, isononyl isononanoate 1%, plant bionic sebum 1%, dicaprylyl carbonate 0.5%; Component C: 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol; Component D: soothing composition 12%, butylene glycol 2%; Remaining water.

[0010] Further preferably, the soothing composition comprises 45% of Aquilariae Lignum Resinatum extract, 10% of Juniper (Juniperus Communis) fruit extract, and 45% of sodium DNA.

[0011] The present invention also provides a method for preparing the above-mentioned repair composition, comprising the following steps: 1) heating and stirring component A to dissolve to obtain a component A mixture; 2) heating and stirring component B to dissolve to obtain a component B mixture; 3) Add the mixture of component B to the mixture of component A under heating, stir and emulsify, then cool it down for the first time, add component C, mix well, and cool it down again, add component D, mix well, and finally cool it down, and filter the material to obtain the soothing repair composition.

[0012] Preferably, in step 1), the heating temperature is 80°C-85°C; in step 2), the heating temperature is 80°C-85°C.

[0013] Preferably, in step 3), the heating state is maintained at 80°C-85°C; the first cooling is to reduce the temperature to 60°C-65°C; the second cooling is to reduce the temperature to 40°C-45°C; and the final cooling is to reduce the temperature to 35°C-38°C.

[0014] Preferably, in step 3), the stirring speed of the emulsification is 6000 r / min.

[0015] Preferably, the mesh size of the filtration in step 3) is 200 mesh.

[0016] The present invention also provides use of the soothing and repairing composition in the preparation of cosmetics and / or pharmaceutical products.

[0017] The rapid soothing and repairing composition of the dual biomimetic liquid crystal system provided by the present invention has the following significant advantages: 1. This composition utilizes biomimetic liquid crystals. Through scientific formulation and molecular structural design, it accurately mimics the lamellar liquid crystal structure of lipids between stratum corneum cells. This highly ordered structure helps enhance the affinity and permeability of topical preparations, including this composition, to the skin surface. Simultaneously, it mimics the composition and function of the skin's natural moisturizing factor, effectively locking in moisture, increasing skin hydration, and helping to restore and maintain the skin's barrier function. The biomimetic liquid crystal system exhibits enhanced dynamic adaptability, adjusting its state to changes in the external environment, better protecting the skin from external stimuli while promoting the skin's own self-regulation ability. This provides a new technological platform for the development of highly responsive skincare products.

[0018] 2. This composition scientifically applies plant-based bionic sebum, whose components are highly similar to the body's own sebum components. It can fill tiny cracks in the stratum corneum, enhance the skin's barrier function, and prevent the invasion of harmful external substances. Among them, bisabolol can downregulate the release of pro-inflammatory factors by inhibiting the COX-2 pathway and the NF-κB signaling pathway, thereby quickly relieving skin irritation reactions and improving discomfort symptoms such as redness, swelling, and itching. Dipotassium glycyrrhizate can effectively reduce allergic reactions and inflammatory reactions by inhibiting histamine release and cyclooxygenase activity. The two synergistically inhibit the release of inflammatory factors and relieve skin sensitivity reactions. Plant-based bionic sebum achieves multi-dimensional repair and strengthening of the skin barrier through structural complementarity and functional synergy between its components.

[0019] 3. This composition scientifically applies a patented soothing combination, in which agarwood extract and juniper fruit extract synergistically inhibit key signaling pathways such as NF-κB, COX-2, and MAPK, systematically regulating the dynamic balance of pro-inflammatory and anti-inflammatory factors to achieve multi-target anti-inflammatory effects; DNA sodium accelerates the repair of damaged cell DNA, promotes keratinocyte proliferation and differentiation, improves the density and integrity of the stratum corneum, and accelerates wound healing.

[0020] 4. This composition, based on the biomimetic principles of skin barrier structure and function, systematically screened and scientifically formulated a "dual biomimetic system" consisting of a biomimetic liquid crystal system and a plant-based biomimetic sebum system. This system, further synergistically combined with a soothing component, creates an innovative system that integrates "structural biomimetic reconstruction, functional repair and enhancement, and bioactivity regulation." This system not only improves product stability, spreadability, and user experience at the macro level, but also achieves precise repair, deep nourishment, and long-lasting stabilization of the skin barrier at the micro level. Experimental data shows that this formula rapidly reduces redness within 15 minutes, significantly reduces the red zone value in VISIA® imaging (by 10.90%), and significantly increases skin hydration (with approximately 26% higher moisturizing efficiency than a single biomimetic system). After seven days of continuous use, transepidermal water loss (TEWL) decreased by 16.09%, demonstrating superior repair efficacy compared to a single biomimetic system.

[0021] 5. Selected mild ingredients, tested and certified for sensitive skin, suitable for all skin types, especially fragile and sensitive skin, and ensure that the product has the characteristics of high safety, mildness and non-irritation. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 1 is a graph showing changes in stratum corneum water content provided in Experimental Example 1, wherein a is the result of Example 1, b is the result of Comparative Example 1, c is the result of Comparative Example 2, and d is the result of Comparative Example 3; Figure 2 2 is a graph showing changes in transepidermal water loss of the skin provided in Experimental Example 2, wherein a is the result of Example 1, b is the result of Comparative Example 1, c is the result of Comparative Example 2, and d is the result of Comparative Example 3; Figure 3 2 is a graph showing changes in skin epidermal thickness provided in Experimental Example 2, wherein a is the result of Example 1, b is the result of Comparative Example 1, c is the result of Comparative Example 2, and d is the result of Comparative Example 3; Figure 4 This is an example diagram of the change in skin epidermal thickness provided in Experimental Example 2, where the left image is at D0, the middle image is at D14, and the right image is at D17 (i.e., 3 days after discontinuation of use); Figure 53 is a diagram of zebrafish tail fin tissue repair efficacy provided by Experimental Example 3, wherein a is the 0h state diagram of the blank control group, b is the 72h state diagram of the blank control group, c is the 0h state diagram of 0.1% Example 1, and d is the 72h state diagram of 0.1% Example 1; Figure 6 : This is a comparison chart of the cell scratch migration experiment area provided in Experimental Example 4, wherein a is the 0-hour state chart of the blank control group, b is the 0-hour state chart of 0.01% Example 1, c is the 24-hour state chart of the blank control group, and d is the 24-hour state chart of 0.01% Example 1; Figure 7 : is a graph showing changes in red zone values ​​provided in Experimental Example 5, wherein a is the result of Example 1, b is the result of Comparative Example 1, c is the result of Comparative Example 2, and d is the result of Comparative Example 3; Figure 8 : is a graph showing the change in skin color a value provided in Experimental Example 5, wherein a is the result of Example 1, b is the result of Comparative Example 1, c is the result of Comparative Example 2, and d is the result of Comparative Example 3; Figure 9 This is the facial red zone improvement example 1 provided in Experimental Example 5, where A is the state before use and B is the state 15 minutes after use; Figure 10 This is the facial red zone improvement example 2 provided in Experimental Example 5, where A is the D0 state and B is the D14 state; Figure 11 : is a graph showing changes in skin glossiness provided in Experimental Example 6, wherein a is the result of Example 1, b is the result of Comparative Example 1, c is the result of Comparative Example 2, and d is the result of Comparative Example 3; Figure 12 : is a graph of skin elasticity changes provided by Experimental Example 6, wherein a is the result of Example 1, b is the result of Comparative Example 1, c is the result of Comparative Example 2, and d is the result of Comparative Example 3; Figure 13 This is a graph showing the negative control results in the chicken embryo chorioallantoic membrane test in Experimental Example 7, wherein the left graph is before sample addition and the right graph is after sample addition; Figure 14 This is a graph showing the positive control results in the chicken embryo chorioallantoic membrane test in Experimental Example 7, wherein the left graph is before sample addition and the right graph is after sample addition; Figure 15 This is a result diagram of Example 1 in the chicken embryo chorioallantoic membrane test of Experimental Example 7, wherein the left picture is before sample addition and the right picture is after sample addition. DETAILED DESCRIPTION

[0023] The present invention is further described below by way of examples, but the present invention is not limited to the scope of the examples. Based on the examples in the present invention, any variation of the present invention by those skilled in the art without creative work falls within the scope of protection of the present invention.

[0024] The raw materials and experimental equipment used in the following embodiments, comparative examples and experimental examples are all commonly used raw materials and experimental equipment in this field. For example, bionic liquid crystal was purchased from Shanghai Zhina Biotechnology Co., Ltd., and plant bionic sebum was purchased from Shanghai Zhina Biotechnology Co., Ltd. The specific sources of other raw materials and experimental equipment are not repeated here.

[0025] Example 1-3: The composition is composed of weight percentages as shown in Table 1.

[0026] Table 1 Example formulation design

[0027] Comparative Examples 1-3: The composition is composed of weight percentages as shown in Table 2.

[0028] Table 2 Comparative Example Formula Design

[0029] The specific preparation steps of the compositions provided in the three embodiments and three comparative examples are as follows: 1) accurately weigh components A and B and heat them to 80-85°C respectively; 2) stir component A with a stirrer until the powder is completely dissolved, keep warm for 20 minutes, and obtain a component A mixture for later use; 3) stir component B with a stirrer until the powder is completely dissolved, and obtain a component B mixture for later use; 4) after the component A mixture is kept warm, add the component B mixture to the component A mixture at 80-85°C, stir and emulsify for 5 minutes; 5) when the emulsification is completed and the temperature is lowered to 60-65°C, add component C, stir evenly, and continue to cool; 6) after cooling to 40-45°C, add component D, stir evenly, and continue to cool; 7) filter and discharge the material when the temperature is lowered to 35-38°C.

[0030] Experimental testing demonstrates that the composition prepared in Example 1 has a superior immediate soothing and redness-reducing effect compared to Comparative Examples 1-3. This further demonstrates that the dual biomimetic system of biomimetic liquid crystal and plant sebum can synergistically accelerate the penetration of active ingredients into the deep layers of the skin, achieving a repair effect. It also moisturizes, nourishes, and promotes wound healing, while being gentle and non-irritating. The compositions prepared in Example 1 and Comparative Examples 1-3 were tested, and the specific efficacy tests are as follows: Experimental Example 1: Moisturizing Effect Test Testing instrument: Skin moisture tester Corneometer CM825; Number of subjects and conditions: 40 normal female subjects aged 18-45 years (positive lactate sting test); Test area: Subject's face; The test samples were: the compositions prepared in Example 1 and Comparative Examples 1-3; Test steps: Day 1 (D0): ① Register the subject information, provide education and training, and the researchers will distribute the research plan. At the same time, they will provide a detailed and comprehensive introduction to all aspects of the research, including the purpose, methods, introduction, informed consent, follow-up of the subjects during the research period, adverse reactions of the product and their treatment measures, etc.

[0031] ② Participants will be screened by researchers, meet the inclusion and exclusion criteria, and sign the volunteer agreement before participating in the test. They must stop using similar functional cosmetics 48 hours before the start of the test, and must not engage in any other activities that may affect the screening assessment; ③ Subjects who meet the inclusion criteria can be included in the study. Stop using similar functional cosmetics 48 hours before the start of the test. On the day of the test, wash the test area with clean water and dry the skin with a lint-free paper towel. After sitting in a constant temperature and humidity environment (temperature 21.0±1.0 ℃, relative humidity 50%±10% RH) for 30 minutes, collect the basic skin values ​​of the subjects: red zone value, skin stratum corneum water content, skin transepidermal water loss, skin color a value, glossiness, skin elasticity, and then randomly divide the subjects into 4 groups, of which Group A uses the composition of Example 1, and the other three groups use the compositions of Comparative Examples 1-3 respectively. Collect the red zone value of the subjects 15 minutes after use, distribute samples and product instructions, and provide written test precautions; ④The subjects left the laboratory after receiving the product and instructions for use.

[0032] Day 7 (D7): ① The subjects returned for a follow-up visit. They cleaned their faces with a cleanser and dried their skin with a lint-free paper towel. After sitting quietly for 30 minutes in a constant temperature and humidity environment (temperature 21.0±1.0℃, relative humidity 50%±10% RH), the subjects' skin data on Day 7 were collected: red zone value, skin stratum corneum water content, skin transepidermal water loss, skin color a value, glossiness, and skin elasticity. ②The subject signs out.

[0033] Day 14 (D14): ① The subjects returned for a follow-up visit. They cleaned their faces with a cleanser and dried their skin with a lint-free paper towel. After sitting quietly for 30 minutes in a constant temperature and humidity environment (temperature 21.0±1.0℃, relative humidity 50%±10% RH), the subjects' D14 skin data were collected: red zone value, skin stratum corneum water content, skin transepidermal water loss, skin color a value, glossiness, and skin elasticity. ②The subject returns the product and weighs it.

[0034] The skin moisturizing effect of the product was evaluated by testing the changes in skin moisture content on days 0, 7, and 14. The test data changes are shown in Table 3: Table 3 Changes in skin moisture content

[0035] from Figure 1 It can be seen that compared with before using the composition, the water content of the stratum corneum of the skin in Example 1 showed a significant upward trend, with the average value increasing by 8.71% and 14.91% on the 7th day and 14th day respectively, and the improvement on the 7th day showed a significant difference (0.01≤ p <0.05, and showed extremely significant differences on the 14th day (0.001≤ p <0.01). Compared with before using the composition, the water content of the stratum corneum of the skin of Comparative Example 1 increased only slightly, with the average value increasing by 1.2% on the 7th day and the 14th day respectively ( p >0.05) and 10.45% (0.01≤ p <0.05). Compared with before using the composition, the average water content of the stratum corneum of Comparative Example 2 and Comparative Example 3 increased by 5.53% and 7.79% on the 7th day, respectively, with no significant difference. On the 14th day, it increased by 12.07% and 11.08%, respectively, both showing significant differences (0.01≤ p <0.05). This shows that the dual bionic system has a synergistic effect and its moisturizing effect is better than that of a single bionic technology.

[0036] Experimental Example 2: Repair Effect Test Testing instruments: Tewameter TM300 skin water loss tester, Ultrascan UC22 skin ultrasound diagnostic instrument; Number of subjects and conditions: 40 normal female subjects aged 18-45 years; Test area: Subject's face; Test steps: Same as those in Experimental Example 1.

[0037] The skin repair efficacy of the product was evaluated by testing the transepidermal water loss and epidermal thickness of the skin disease at 0, 7, and 14 days, as well as the changes in epidermal thickness of the skin 3 days after discontinuation of use. The test data changes are shown in Tables 4 and 5: Table 4 Changes in transepidermal water loss

[0038] Table 5 Changes in skin epidermal thickness

[0039] from Figure 2 It can be seen that compared with the results before using the composition, the transepidermal water loss of Example 1 showed a significant downward trend, with the average values ​​decreasing by 16.09% and 18.89% on the 7th and 14th days respectively, and showing a significant difference (0.001≤ p <0.01), with an improvement effect superior to that of Comparative Examples 1-3. Compared to before use of the composition, the transepidermal water loss measurements of Comparative Examples 2 and 3 improved by 15.16% and 15.96% on day 14, respectively, both superior to Comparative Example 1 (14.30%).

[0040] from Figure 3 and Figure 4 It can be seen that compared with the results before using the composition, the skin epidermal thickness measurement results of Example 1 showed a significant upward trend, and the average values ​​increased by 11.26% on the 7th day and 14th day, respectively. p <0.05) and 24.14% ( p <0.01), and the skin epidermal thickness remained at a level similar to that on the 14th day after 3 days of discontinuation ( p>0. 5%), demonstrating a degree of sustainability and stability in its repair effects. While the epidermal thickness of Comparative Example 1 increased by only 5.19% (on day 7) and 12.01% (on day 14), Comparative Example 2 saw increases of 7.08% and 15.01%, respectively; and Comparative Example 3 saw increases of 7.67% and 17.07%, respectively. These discrepancies directly validate the synergistic effect of the dual biomimetic system in skin barrier repair, significantly outperforming a single biomimetic technology.

[0041] Experimental Example 3: Tissue Repair Effect Test Testing instruments: ZA-D5 five-layer single-row independent culture unit, SZ680 continuous zoom stereo microscope, ZXSD-A1090 biochemical incubator, SOP 1 / 10,000 electronic scale; Experimental fish: wild-type AB zebrafish; Test steps: 1) Grouping and Control: Sample dosage grouping: 0.1% of Example 1, blank control group: standard dilution water.

[0042] 2) Main Procedures: Normally developed 72 hpf zebrafish larvae were randomly selected and anesthetized with 0.02% tricaine. The caudal fin was removed and the larvae were incubated in a 28°C biochemical incubator for 2 h. Images were taken under a stereomicroscope. The larvae were then transferred to a 96-well plate, 20 per group. 400 μL of the sample solution was added to each well. The plates were incubated in a 28°C biochemical incubator for 72 h. Images were taken under a stereomicroscope, and the anterior-posterior growth of the caudal fin was measured using Image J. A blank control group and the group described in Example 1 were set up.

[0043] Test judgment basis: Tail fin regeneration growth rate (%): Compared with the blank control group, the tail fin regeneration length of the sample group is longer, and the result* p <0.05, significant difference, indicating that the sample has the ability to promote the repair of zebrafish larvae tail fin tissue at this experimental concentration.

[0044] Test results: like Figure 5 As shown in Table 6, the zebrafish tail fin was treated with the sample for 72 hours after removal and the growth rate of the tail fin was recorded by taking photos. The blank control group was treated with standard dilution water. Under the same conditions, as shown in Table 6, the relative growth rate of the tail fin of zebrafish treated with 0.1% Example 1 for 72 hours was 110.21±2.16%, an increase of 10.21% (compared with the blank control group (100.00±2.03%)). p <0.01). This indicates that under this experimental condition, 0.1% of Example 1 has the effect of promoting tissue repair, and the effect is significant.

[0045] Table 6 Effect of Example 1 on the repair of zebrafish caudal fin tissue

[0046] Note: Comparison between sample group and blank control group:* p <0.05,** p <0.01,*** p <0.001.

[0047] Experimental Example 4: Wound healing effect test Testing instruments: inverted microscope, cell counter, multifunctional microplate reader, carbon dioxide incubator, centrifuge, 6-well culture plate; Experimental cells: human immortalized keratinocytes (HaCaT); Test steps: 1) Grouping and Control: Sample dosage grouping: serum-free culture medium containing 0.01% of Example 1; blank control group: serum-free culture medium.

[0048] 2) Main Procedure: Cells in the logarithmic growth phase were digested and centrifuged. A cell suspension of appropriate density was prepared in complete culture medium. The cells were seeded into 6-well culture plates according to the experimental design and incubated in a 37°C, 5% CO2 incubator for 24 ± 2 hours. When the cells reached a confluence of 98% or higher, a straight line was drawn perpendicularly to the bottom of the 6-well plate using a sterile pipette tip. After the line was drawn, the original culture medium was discarded and the cells were washed three times with DPBS. Samples were added according to the experimental group. Serum-free culture medium containing 0.01% sample was added to the sample group, while serum-free culture medium was added to the blank control group. 2 mL of sample was added to each well. After sample addition, three randomly selected fields of view were photographed using an inverted microscope. After incubation in a 37°C, 5% CO2 incubator for 24 ± 2 hours, images were taken using an inverted microscope at the same field of view as at 0 hours.

[0049] The scratch area was analyzed using Image J software, and the cell migration ability was expressed as migration rate: cell migration rate (%) = (0 h scratch area - 24 h scratch area) / 0 h scratch area × 100%.

[0050] Test judgment basis: Cell migration rate: Compared with the blank control group, the cell scratch image analysis of the sample group showed that the cell scratch area of ​​the sample group was significantly reduced, and by analyzing the cell migration rate data, the migration rate of the sample group was significantly increased compared with the migration rate of the blank control group (* indicates p <0.05, ** indicates p <0.01), then under the conditions of this experiment, the sample at this concentration has the effect of promoting cell regeneration and wound healing.

[0051] Test results: like Figure 6 As shown, after 24 hours of treatment with 0.01% Example 1, the scratch area was significantly reduced, and the cell migration rate was 36.02±2.55%, which was higher than that of the blank control group (BC, 29.47±1.69%), and there was a significant difference (* p <0.05). This indicates that under this experimental condition, 0.1% of Example 1 acting on cells for 24 hours showed a significant promoting effect on cell migration, and had the efficacy of promoting cell regeneration and wound healing, and the effect was significant.

[0052] Table 7 Effect of Example 1 on cell migration rate

[0053] Note: Comparison between sample group and blank control group:* p <0.05,** p <0.01,*** p <0.001.

[0054] Experimental Example 5: Soothing Effect Test Testing instruments: Facial image analyzer VISIA and skin color test probe Skin-ColorimeterCL400; Number of subjects and conditions: 40 normal female subjects aged 18-45 years; Test area: Subject's face; Test steps: Same as those in Experimental Example 1.

[0055] The soothing effect of the product was evaluated by testing the changes in redness value and skin color a value at 0, 7, and 14 days. The test data changes are shown in Tables 8 and 9: Table 8 Changes in red zone values

[0056] Table 9 Changes in skin color a value

[0057] from Figure 7 、 Figure 8 、 Figure 9 、 Figure 10 It can be seen that compared with before using the composition, the facial redness score of Example 1 showed a significant downward trend, with the average value decreasing by 10.90%, 13.90% and 18.24% at 15 minutes, 7 days and 14 days respectively, and showing obvious significant differences (0.001≤ p <0.01). The skin color a value showed a downward trend, with its average value decreasing by 11.28% and 17.09% on the 7th and 14th days respectively, and showing a significant difference on the 7th day (0.001≤ p <0.01, and showed extremely significant differences on day 14 ( p <0.001). Example 1 has better immediate and long-lasting soothing effects on sensitive skin than Comparative Examples 1-3.

[0058] Experimental Example 6: Nourishing Effect Test Testing instruments: Skin gloss tester CL200 and skin elasticity probe; Number of subjects and conditions: 40 normal female subjects aged 18-45 years; Test area: Subject's face; Test steps: Same as those in Experimental Example 1.

[0059] The nourishing effect of the product was evaluated by testing the changes in skin gloss and skin elasticity on days 0, 7, and 14. The test data changes are shown in Tables 10 and 11: Table 10 Changes in skin glossiness

[0060] Table 11 Changes in skin elasticity

[0061] from Figure 11 、 Figure 12 It can be seen that compared with the skin glossiness before using the composition, the skin glossiness of Example 1 showed an upward trend, with the average value increasing by 7.77% and 10.87% on the 7th day and the 14th day, respectively, and the improvement on the 14th day showed a significant difference (0.01≤ p <0.05). The average skin elasticity increased by 7.34% and 11.48% on the 7th and 14th days, respectively, and the improvement on the 14th day showed a significant difference (0.001≤ p <0.01). After 14 days of continuous use, Example 1 showed better improvements in skin gloss and skin elasticity than Comparative Examples 1-3.

[0062] Experimental Example 7 Irritation Test Experimental materials and equipment: chicken embryos (SPF-grade white chicken), stereo microscope, incubator, analytical balance, timer; Negative control (NC): 0.9% NaCl; positive control (PC): 0.1 mol / L NaOH; sample group: Example 1; Test steps: 1) Chorioallantoic Membrane (CAM) Preparation: Purchase chick embryos of appropriate age. At 9 days of incubation, inspect and discard any defective embryos. Mark the location of the air cells on the surface of the eggshell of a healthy chick. Peel back the shell to expose the white chorioallantoic membrane. Carefully remove the inner membrane with forceps, ensuring that the vascular membrane is not damaged.

[0063] 2) Stimulation scoring method: 0.3 mL of the sample was dripped directly onto the CAM surface in each group of 6 chick embryos. The CAM reaction was observed and the time of bleeding, coagulation, and vascular melting within 5 minutes was recorded, as well as the degree of reaction.

[0064] Endpoint Scoring Method: For each group of six chick embryos, apply 0.3 mL of the prepared sample directly to the CAM surface and observe the CAM reaction. After 3 minutes, gently rinse with saline to remove the test substance. Observe the three reactions and their severity within approximately 30 seconds of rinsing: bleeding, coagulation, and vascular lysis. If all six chick embryos demonstrate a moderate or higher score for at least one reaction (total score = 212), the experiment should be repeated.

[0065] Positive and negative controls were scored using the stimulation scoring method, while samples were scored using the endpoint scoring method.

[0066] ③Irritation score method (IS): Record the time of occurrence and reaction degree of each test endpoint, and calculate the irritation score (IS) using the following formula, with the result rounded to two decimal places:

[0067] sec H, sec L, and sec C represent the average time (in seconds) to the onset of bleeding, vascular melting, and coagulation, respectively, observed on the CAM membrane.

[0068] Table 12 Evaluation of stimulation scoring method results

[0069] Endpoint scoring (ES): score for each chick embryo = the sum of the degree of bleeding, coagulation and vascular melting observed in each chick embryo; End point score (ES) = the sum of the scores of 6 chicken embryos.

[0070] Table 13 Evaluation of stimulation scoring method results

[0071] Experimental results The scoring results of the control group and the test samples are shown in Tables 14 and 15 below.

[0072] Table 14 Scoring results of the control group

[0073] Table 15 Test sample scoring results

[0074] From Tables 14, 15 and Figure 13-15 It can be seen that the ES score of Example 1 is 0, and the irritation classification is non-irritating. According to the judgment rules of the chicken embryo chorioallantoic membrane test, Example 1 is mild and non-irritating.

[0075] From the above test results, it can be seen that the rapid repair composition of the dual bionic liquid crystal system in the present invention can break through the limitations of the slow response of single bionic technology, can quickly soothe sensitive skin, and can fade redness within 15 minutes. It also has efficient moisturizing and barrier repair functions, can promote cell regeneration and wound healing, provide deep nourishment for the skin, and is gentle and non-irritating.

[0076] Finally, it should be noted that the above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the examples provided, those skilled in the art may modify or substitute equivalents for the technical solutions of the present invention as needed without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A soothing and repairing composition comprising the following components in percentage by weight: Component A: Dipropylene Glycol 1-3%, Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer 1-2%, Polysorbate 60 0.05-0.1%, Sorbitan Isostearate 0.05-0.1%, Glycerin 0.5-1.5%, Disodium EDTA 0.01-0.05%; Component B: biomimetic liquid crystal 1-5%, cyclopentasiloxane 1-5%, squalane 1-4%, ethylhexyl palmitate 1-2%, isononyl isononanoate 0.5-2%, plant biomimetic sebum 0.5-1.5%, dicaprylyl carbonate 0.1-0.9%; Component C: 0.1-1% p-hydroxyacetophenone, 0.1-1% 1,2-hexanediol; Component D: soothing composition 10-15%, butylene glycol 1-3%; Remaining water.

2. The soothing and repairing composition according to claim 1, wherein The bionic liquid crystal comprises 55-75% of cetearyl alcohol, 10-20% of phytosterols, 10-20% of glyceryl isostearate and 1-5% of hydrogenated lecithin.

3. The soothing and repairing composition according to claim 1, wherein: The plant biomimetic sebum comprises 50-60% caprylic / capric triglyceride, 25-30% phytosterols, 10-20% glyceryl stearate, 0.1-1% tocopheryl acetate, 0.1-1% hydrogenated lecithin, 0.1-1% bisabolol and 0.1-1% dipotassium glycyrrhizate.

4. The soothing and repairing composition according to claim 1, wherein: The soothing composition comprises: 40-50% agarwood extract, 5-15% juniper fruit extract and 40-50% sodium DNA.

5. The soothing and repairing composition according to claim 1, wherein: The soothing and repairing composition comprises the following components in percentage by weight: Component A: Dipropylene Glycol 2%, Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer 1.5%, Polysorbate 60 0.08%, Sorbitan Isostearate 0.08%, Glycerin 1%, Disodium EDTA 0.03%; Component B: Bionic liquid crystal 3%, cyclopentasiloxane 3%, squalane 2%, ethylhexyl palmitate 1.5%, isononyl isononanoate 1%, plant bionic sebum 1%, dicaprylyl carbonate 0.5%; Component C: 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol; Component D: soothing composition 12%, butylene glycol 2%; Remaining water.

6. The soothing and repairing composition according to claim 5, characterized in that: The soothing composition includes Agarwood Extract 45%, Juniperus chinensis Fruit Extract 10%, and Sodium DNA 45%.

7. A method for preparing the soothing and repairing composition according to any one of claims 1 to 6, comprising the following steps: 1) heating and stirring component A to dissolve to obtain a component A mixture; 2) heating and stirring the B component to dissolve it to obtain a B component mixture; 3) Add the mixture of component B to the mixture of component A under heating, stir and emulsify, then cool it down for the first time, add component C, mix well, and cool it down again, add component D, mix well, and finally cool it down, and filter the material to obtain the soothing repair composition.

8. The preparation method according to claim 7, wherein In step 1), the heating temperature is 80°C-85°C; In step 2), the heating temperature is 80°C-85°C; In step 3), the heating state is maintained at 80°C-85°C; the first cooling is to reduce the temperature to 60°C-65°C; the second cooling is to reduce the temperature to 40°C-45°C; and the final cooling is to reduce the temperature to 35°C-38°C.

9. The preparation method according to claim 7, wherein In step 3), the stirring speed of the emulsification is 6000 r / min; and the mesh size of the filtration in step 3) is 200 mesh.

10. Use of the soothing and repairing composition according to any one of claims 1 to 6 in the preparation of cosmetics and / or pharmaceutical products.

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