Method for promoting development of fescue fetal inflorescence through plant hormone formula

Abstract

The invention belongs to the field of blue fescue development, and provides a method for promoting blue fescue fetal inflorescence development through a plant hormone formula, and the method comprises the following steps: selecting a blue fescue parent plant before flower bud differentiation, preparing a plant hormone solution which contains abscisic acid and gibberellins, the concentration ratio of the abscisic acid to the gibberellins is 3: 1, and the plant hormone formula is used for promoting the blue fescue fetal inflorescence development. The maternal plants of the blue fescue are subjected to hormone irrigation treatment, specifically, the maternal plants of the blue fescue are irrigated once every three days, an abscisic acid solution is irrigated firstly, then a gibberellin solution is irrigated, and the irrigation period lasts for 2 months; according to the method disclosed by the invention, the maternal plant of the fescue is treated by the plant hormones with a specific ratio, so that the generation quantity of fetal buds on the vegetative body fetal inflorescence is remarkably increased, the asexual propagation coefficient of the fescue is rapidly expanded in a relatively short time, and meanwhile, the excellent horticultural characters of the female parent can be kept by propagated offspring; therefore, the market supply capability and the economic benefit of the festuca arundinacea seedlings are effectively improved.

Description

Technical Field

[0001] The invention belongs to the field of blue fescue development, in particular to a method for promoting the development of blue fescue viviparous inflorescences by using a plant hormone formula. Background Art

[0002] Blue fescue is a perennial, cool-season herbaceous plant of the genus Festuca (Festuca glauca) in the Poaceae family. Its ornamental value is primarily reflected in its blue leaves. As a member of the broader category of ornamental grasses, blue fescue, belonging to the blue family of ornamental grasses, has high ornamental value and is being used in increasing numbers in landscape gardens. While the original species of blue fescue can be propagated by sowing, horticultural varieties require division to maintain their characteristics. However, research on the propagation of some horticultural blue fescue varieties is limited. Among them, 'Elijah Blue' blue fescue (Festuca glauca 'Elijah Blue') is one of the most popular horticultural varieties currently on the market. However, the propagation of horticultural blue fescue varieties has been a key factor restricting their large-scale application.

[0003] Traditional propagation methods for blue fescue mainly include seed propagation and division propagation. Although seed propagation is simple and easy, for horticultural varieties, due to the existence of genetic variation, the offspring of seed propagation often find it difficult to maintain the excellent traits of the mother plant. Although division propagation can maintain the characteristics of the variety, its propagation efficiency is low and it is difficult to meet the needs of large-scale garden applications. In addition, some horticultural varieties of blue fescue will exhibit vegetative viviparity under specific growth environments, that is, vegetative buds are directly produced on the inflorescence. These buds can be directly propagated by cuttings to obtain plants identical to the mother plant. However, this naturally occurring vegetative viviparity phenomenon is low in frequency and is greatly affected by environmental factors, making it difficult to stably provide sufficient propagation materials.

[0004] Therefore, those skilled in the art have proposed a method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula to solve the problems raised in the background art. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides a method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula, so as to solve the technical problems in the prior art of low reproduction efficiency and difficulty in maintaining excellent traits of blue fescue horticultural varieties.

[0006] A method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula, comprising the following steps: S1: selecting a blue fescue mother plant before flower bud differentiation;

[0007] S2: preparing a plant hormone solution, wherein the plant hormone solution comprises abscisic acid and gibberellin, and the concentration ratio of abscisic acid to gibberellin is 3:1;

[0008] S3: The blue fescue mother plant is irrigated with hormones, specifically, irrigated once every 3 days, first with an abscisic acid solution, then with a gibberellin solution, and the irrigation cycle lasts for 2 months.

[0009] Preferably, in step S2, the concentration of the abscisic acid solution is 300 ppm, the concentration of the gibberellin solution is 100 ppm, and the weight of the required hormones is calculated using the following algorithm during the preparation process: m=C×V, where m is the weight of the required hormones in g, C is the target concentration in ppm, and V is the volume of the solution in L.

[0010] Preferably, the amount of hormone solution irrigated in step S3 is adjusted according to the size and growth condition of the blue fescue mother plant. The specific adjustment algorithm uses the following formula to estimate the amount of hormone solution required for each plant: Where V plant is the amount of hormone solution required for each plant, A is the leaf area of ​​the plant, and R is the expected hormone absorption rate.

[0011] Preferably, the blue fescue mother plant is the horticultural cultivar 'Aili' blue fescue.

[0012] Preferably, the time for watering the hormone solution in step S3 is selected in the morning or evening.

[0013] Preferably, while watering the hormone solution in step S3, conventional water and fertilizer management is performed on the blue fescue mother plant. The specific water and fertilizer management plan adopts a water-fertilizer coupling model, specifically G=aW+bF+cWF, where G is the maximum growth rate of the plant, W is the adjusted irrigation water volume, F is the fertilizer amount, and a, b, and c are model parameters.

[0014] Preferably, the method further comprises step S4: after irrigating the hormone solution for 2 months, observing and recording the changes in the number of vegetative buds on the inflorescence of the blue fescue mother plant, and comparing the changes with the control group that was not treated with hormones, using a significant difference test, which may be a t-test, and the statistical calculation formula is:

[0015]

[0016] in, and are the average number of embryos in the treatment group and the control group, s p is the combined standard deviation, n1 and n2 are the sample sizes of the treatment group and the control group, respectively.

[0017] Preferably, in step S4, a statistical method is used to calculate the increase ratio of the number of vegetative embryos, and the specific algorithm formula is:

[0018]

[0019] At the same time, in order to evaluate the long-term effect of hormone treatment, the growth rate ratio GRR is used as an evaluation index, and its calculation formula is:

[0020]

[0021] Preferably, during the watering process, the plant hormone mixture is evenly applied around the root system of the blue fescue plant by drip irrigation or sprinkler irrigation.

[0022] Preferably, the method further comprises step S5: after the blue fescue plants are watered with the plant hormone mixture, the vegetative buds on the inflorescences thereof can be directly used for cutting propagation to obtain asexual propagation offspring having the same horticultural traits as the mother plant.

[0023] Through the above technical solution,

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. The present invention treats the blue fescue mother plant with a specific ratio of plant hormones, significantly increasing the number of viviparous buds produced on the vegetative viviparous inflorescence, rapidly expanding the asexual reproduction coefficient of blue fescue in a relatively short period of time, and ensuring that the offspring can maintain the excellent horticultural traits of the mother plant, thereby effectively improving the market supply capacity and economic benefits of blue fescue seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The figure is a flow chart of the method steps of the present invention. DETAILED DESCRIPTION

[0027] The following embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0028] Example 1: As shown in the attached Figure 1 As shown: The present invention provides a method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula, comprising the following steps: S1: selecting a blue fescue mother plant before flower bud differentiation;

[0029] S2: preparing a plant hormone solution, the plant hormone solution comprising abscisic acid (ABA) and gibberellin (GA3), and the concentration ratio of abscisic acid to gibberellin is 3:1;

[0030] S3: The blue fescue mother plants were treated with hormone irrigation, specifically, watering once every 3 days, first with abscisic acid solution, then with gibberellin solution, and the irrigation cycle lasted for 2 months.

[0031] Preferably, vegetative viviparous buds are produced on the inflorescence of a horticultural variety of blue fescue. However, by applying a certain ratio of endogenous hormones ABA and GA3 to the mother plant, the number of vegetative buds on the inflorescence can be increased. The specific formula is ABA:GA3=3:1 (in ppm). Before flower bud differentiation, the blue fescue is watered once every three days, first with 300 ppm ABA, then with 100 ppm GA3. After two months of continuous watering, the number of bulbils will increase by 15-20% compared to the original amount. Watering the mother plant that produces vegetative viviparous inflorescences can increase the number of viviparous vegetative buds produced to 15-20% of the original amount, thereby increasing the number of vegetative buds in the inflorescence, expanding the coefficient of asexual reproduction, and improving the market share of blue fescue seedlings.

[0032] As can be seen from the above, the present invention effectively promotes the development of blue fescue viviparous inflorescences and increases the number of viviparous buds produced by precisely controlling the hormone ratio and irrigation cycle. Through specific hormone ratios and irrigation methods, the reproductive efficiency of blue fescue is significantly improved while maintaining the excellent traits of horticultural varieties.

[0033] Example 2: As shown in the attached Figure 1 As shown: This embodiment is basically the same as the previous embodiment, except that, in step S2, the concentration of the abscisic acid solution is 300 ppm, the concentration of the gibberellin solution is 100 ppm, and the weight of the required hormones is calculated using the following algorithm formula during the preparation process: m=C×V, wherein m is the weight of the required hormones in g, C is the target concentration in ppm, and V is the volume of the solution in L. The hormone concentration is precisely controlled to ensure the consistency and reliability of the treatment effect, avoid poor treatment effects or plant damage due to improper hormone concentration, and improve the success rate of reproduction.

[0034] Furthermore, the amount of hormone solution irrigated in step S3 is adjusted according to the size and growth condition of the blue fescue mother plant. The specific adjustment algorithm uses the following formula to estimate the amount of hormone solution required for each plant: Where V plant is the amount of hormone solution required for each plant, A is the leaf area of ​​the plant, and R is the expected hormone absorption rate. The amount of hormone irrigation is adjusted individually to meet the needs of different plants and improve the treatment effect. This avoids resource waste or poor treatment effect caused by improper irrigation amount, ensures that each plant can obtain the appropriate amount of hormone treatment, and improves the overall breeding efficiency.

[0035] Furthermore, the mother plant of blue fescue is the horticultural cultivar 'Ellie' blue fescue, which is optimized for the specific variety to improve the pertinence and effectiveness of reproduction.

[0036] Preferably, the time for watering the hormone solution in step S3 is selected in the morning or evening, which avoids hormone decomposition and plant damage that may be caused by watering during high temperature periods, improves the stability and treatment effect of the hormone solution, and reduces the risk of plant damage.

[0037] As can be seen from the above, the present invention clarifies the specific concentrations (300ppm and 100ppm) and preparation algorithms of abscisic acid (ABA) and gibberellin (GA3) solutions to ensure the accuracy of the hormone ratio. The irrigation amount needs to be adjusted according to the plant size and growth conditions to achieve personalized treatment; it stipulates that the irrigation time should be selected in the morning or evening to avoid the potential adverse effects of high temperatures on hormone stability and plants. These measures jointly improve the reproductive efficiency of blue fescue and the maintenance of horticultural traits.

[0038] Example 3: As shown in the attached Figure 1 As shown: On the basis of Example 1, while watering the hormone solution in step S3, the blue fescue mother plant is subjected to conventional water and fertilizer management. The specific water and fertilizer management plan adopts a water and fertilizer coupling model, specifically G=aW+bF+cWF, where G is the maximum growth rate of the plant, W is the amount of irrigation water, F is the amount of fertilizer, and a, b, and c are model parameters. It is determined through experiments that the comprehensive management of the plant's growth environment improves the overall growth quality of the plant, provides a good plant foundation for hormone treatment, and improves the success rate of reproduction.

[0039] Preferably, the method further comprises step S4: after irrigating the hormone solution for 2 months, observing and recording the changes in the number of vegetative buds on the inflorescence of the blue fescue mother plant, and comparing the changes with the control group that was not treated with hormones, using a significant difference test, which may be a t-test, and the statistical calculation formula is:

[0040]

[0041] in, and are the average number of embryos in the treatment group and the control group, s p is the combined standard deviation, n1 and n2 are the sample sizes of the treatment group and the control group respectively. This claim claims that by introducing the significance test of difference, it can more accurately judge whether the hormone treatment is truly effective, avoid misjudgment due to random errors or sample differences, and make the analysis of experimental results more transparent and repeatable. Through scientific statistical analysis, it can more accurately evaluate the actual effect of hormone treatment on the development of blue fescue viviparous inflorescences, and provide data support for optimizing hormone formulas and treatment methods.

[0042] Specifically, in step S4, a statistical method is used to calculate the increase ratio of the number of vegetative embryo buds, and the specific algorithm formula is:

[0043]

[0044] At the same time, in order to evaluate the long-term effect of hormone treatment, the growth rate ratio GRR is used as an evaluation index, and its calculation formula is:

[0045] By introducing the growth rate ratio (GRR), the combined effects of hormone treatment on the growth and reproduction of blue fescue can be more comprehensively evaluated. By adding a dual evaluation of ratio and growth rate ratio, the effect of hormone treatment can be more comprehensively reflected, providing a more comprehensive reference basis for optimizing hormone formulas and treatment methods, helping to improve the long-term stability and reliability of the method in practical applications, and ensuring that blue fescue can benefit during both reproduction and growth.

[0046] Specifically, during the irrigation process, the plant hormone mixture is evenly applied around the root system of the blue fescue plant by drip irrigation or sprinkler irrigation. The specific irrigation method (drip irrigation or sprinkler irrigation) is clarified to ensure that the plant hormone mixture can be evenly applied around the root system of the plant, thereby improving the uniformity and consistency of the treatment effect. The drip irrigation or sprinkler irrigation method can reduce the waste of hormone solution, improve resource utilization efficiency, and avoid inconsistent plant growth due to excessively high or low local concentrations.

[0047] Preferably, the method further includes step S5: after the blue fescue plant is watered with the plant hormone mixture, the vegetative buds on the inflorescence can be directly used for cutting propagation to obtain asexual propagation offspring with the same horticultural traits as the mother plant. By combining hormone treatment with cutting propagation, not only the propagation efficiency is improved, but also the excellent traits of the horticultural variety can be effectively maintained. Through cutting propagation, the propagation coefficient of blue fescue can be rapidly expanded, the propagation efficiency can be improved, and the needs of large-scale garden applications can be met, ensuring that the propagation offspring can maintain the same excellent traits as the mother plant, thereby improving the application value of blue fescue in garden landscapes and enhancing the competitiveness of this method in the market.

[0048] From the above, it can be seen that the present invention combines conventional water and fertilizer management with a water-fertilizer coupling model while watering the plant hormone solution. By adjusting the irrigation water volume and fertilizer application amount to maximize the plant growth rate, a good growth foundation is provided for hormone treatment, thereby improving the reproduction success rate. A difference significance test (such as a t-test) is introduced to scientifically evaluate the changes in the number of vegetative buds on the blue fescue inflorescence after hormone treatment, and the reliability of the results is ensured through specific statistical formulas, providing data support for optimizing the hormone formula. By adding two quantitative indicators, the proportion and growth rate ratio (GRR), the short-term and long-term effects of the hormone treatment are comprehensively evaluated, making the evaluation method more scientific and comprehensive.

[0049] The embodiments of the present invention are given for the purpose of illustration and description. Although the embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Those skilled in the art can change, modify, replace and modify the above embodiments within the scope of the present invention. In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. The meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0050] In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "connect," "fixed," etc. should be understood broadly. For example, they may refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0051] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0052] In the description of this specification, the reference terms "one embodiment", "some embodiments", "examples", "specific examples" or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0053] In the drawings of the embodiments disclosed in the present invention, only the structures involved in the embodiments disclosed in the present invention are involved. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0054] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula, characterized in that: The method comprises the following steps: S1: selecting a blue fescue mother plant before flower bud differentiation; S2: preparing a plant hormone solution, wherein the plant hormone solution comprises abscisic acid and gibberellin, and the concentration ratio of abscisic acid to gibberellin is 3:1; S3: The blue fescue mother plant is irrigated with hormones, specifically, irrigated once every 3 days, first with an abscisic acid solution, then with a gibberellin solution, and the irrigation cycle lasts for 2 months.

2. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 1, characterized in that: In step S2, the concentration of the abscisic acid solution is 300 ppm, the concentration of the gibberellin solution is 100 ppm, and the weight of the required hormones is calculated using the following algorithm formula during the preparation process: m=C×V, where m is the weight of the required hormones in g, C is the target concentration in ppm, and V is the solution volume in L.

3. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 1, characterized in that: The amount of hormone solution irrigated in step S3 is adjusted according to the size and growth condition of the blue fescue mother plant. The specific adjustment algorithm uses the following formula to estimate the amount of hormone solution required for each plant: Where V plant is the amount of hormone solution required for each plant, A is the leaf area of ​​the plant, and R is the expected hormone absorption rate.

4. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 1, characterized in that: The blue fescue mother plant is the horticultural cultivar 'Aili' blue fescue.

5. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 3, characterized in that: The time for watering the hormone solution in step S3 is selected in the morning or evening.

6. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 5, characterized in that: While watering the hormone solution in step S3, conventional water and fertilizer management is performed on the blue fescue mother plant. The specific water and fertilizer management plan adopts a water and fertilizer coupling model, specifically G=aW+bF+cWF, where G is the maximum growth rate of the plant, W is the adjusted irrigation water volume, F is the fertilizer amount, and a, b, and c are model parameters.

7. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 1, characterized in that: The method further comprises step S4: after irrigating the plant with the hormone solution for 2 months, observing and recording the change in the number of vegetative buds on the inflorescence of the blue fescue mother plant, and comparing the result with that of the control group not treated with the hormone, using a significant difference test, which may be a t-test, and the statistical calculation formula is: in, and are the average number of embryos in the treatment group and the control group, s p is the combined standard deviation, n1 and n2 are the sample sizes of the treatment group and the control group, respectively.

8. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 7, characterized in that: In step S4, a statistical method is used to calculate the increase ratio of the number of vegetative embryo buds, and the specific algorithm formula is: At the same time, in order to evaluate the long-term effect of hormone treatment, the growth rate ratio GRR is used as an evaluation index, and its calculation formula is:

9. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 6, characterized in that: During the watering process, the plant hormone mixture is evenly applied around the root system of the blue fescue plant by drip irrigation or sprinkler irrigation.

10. The method for promoting the development of blue fescue viviparous inflorescences using a plant hormone formula as claimed in claim 8, characterized in that: The method further comprises step S5: after the blue fescue plants are watered with the plant hormone mixture, the vegetative buds on the inflorescences thereof can be directly used for cutting propagation to obtain asexual propagation offspring having the same horticultural traits as the mother plant.

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