Method for improving diapause egg rate of silkworm germplasm resources

Abstract

The invention relates to a method for improving the diapause egg rate of silkworm germplasm resources, which comprises the following steps: during the period from pupation completion to silkworm moth eclosion, illumination period regulation of 8-hour illumination and 16-hour darkness is adopted, a light source does not contain an ultraviolet wave band, the illumination intensity is 350lx-600lx, and the light source is uniformly distributed; the temperature in the illumination period is maintained at 26-28 DEG C, the temperature in the dark period is reduced to 23-25 DEG C to form a day-night temperature difference, and the temperature change and the illumination period are switched synchronously or adjusted gradually in stages; the relative humidity in the illumination period is controlled to be 70-75%, the relative humidity in the dark period is improved to be 75-85%, and the humidity change is synchronous with illumination period switching. The method is mainly used for silkworm germplasm production by regulating and controlling the photoperiod to cooperate with temperature and humidity changes and simulating a natural autumn cooling signal to induce diapause, the storable and planned production of silkworm eggs is guaranteed, and resource waste is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of silkworm breeding, and more specifically, the invention relates to a method for improving the diapause egg rate of silkworm germplasm resources. Background Art

[0002] The silkworm (Bombyx mori), an economic insect that overwinters in diapause, lays its eggs in a diapause state. Its eggs can be divided into diapause eggs (commonly known as black eggs) and non-diapause eggs (commonly known as live eggs). Diapause eggs require activation before hatching, making them suitable for long-term storage and planned production. Non-diapause eggs, on the other hand, continue to develop and hatch approximately 10 days after laying and cannot enter diapause.

[0003] In recent years, the high incidence of non-diapause eggs in silkworm germplasm resources has become a core issue restricting the development of the industry, such as: 1. Quality defects: non-diapause eggs are poor quality eggs, resulting in a significant decrease in the qualified rate of silkworm seed batches; 2. Economic losses: The number of silkworm seeds eliminated due to non-diapause eggs in summer remains high, and the annual losses of silkworm seed farms alone amount to tens of millions of yuan (including breeding, testing, storage, destruction costs and potential loss of profits); 3. Industrial risks: Unplanned hatching disrupts the production cycle, forcing companies to invest additional funds in the research and development or introduction of new varieties.

[0004] At present, the sericulture industry urgently needs a method to efficiently control the incidence of non-diapause eggs, so as to increase the proportion of diapause eggs (good quality egg rate), ensure the storability and planned productivity of silkworm seeds, reduce resource waste, reduce economic losses, and enhance the international competitiveness of the silk industry. Summary of the Invention

[0005] An object of the present invention is to address at least the above-mentioned drawbacks and to provide at least the advantages which will be described hereinafter.

[0006] The present invention provides a method for improving the diapause egg rate of silkworm germplasm resources, one purpose of which is to suppress the occurrence of non-diapause eggs and improve the diapause egg rate.

[0007] The present invention provides a method for improving the diapause egg rate of silkworm germplasm resources. The method comprises the following steps: regulating the period from the completion of pupation to the emergence of silkworm moths by adopting a light cycle of 8 hours of light: 16 hours of darkness (8L:16D), wherein the light source does not contain an ultraviolet band; the light intensity is 350lx-600lx, and the light source is evenly distributed; the temperature is maintained at 26-28°C during the light period, and the temperature is reduced to 23-25°C during the dark period, thereby forming a day-night temperature difference; the temperature change is switched synchronously with the light cycle or adjusted gradually in stages; the relative humidity is 70-75% during the light period, and the relative humidity is increased to 75-85% during the dark period; and the humidity change is switched synchronously with the light cycle.

[0008] Preferably, the temperature switching between the light period and the dark period adopts a staged gradual adjustment; 30 minutes before the start of the light period, the temperature is started from 23-25 ​​° C to 26-28 ° C step heating program, the step heating rate is 1 ° C / 10 minutes; 30 minutes before the start of the dark period, the temperature is started from 26-28 ° C to 23-25 ​​° C step cooling program, the step cooling rate is 1 ° C / 10 minutes; the temperature and humidity sensors are placed in the diagonal position and the center area of ​​the silkworm room respectively, and the data are averaged as the basis for execution.

[0009] Preferably, uniform distribution of light sources is achieved in the following manner: a cold white light LED light source array is installed on the top of the silkworm room at an inclination angle of 45°, and the distance between the light sources is 1.2 times the height of the illuminated surface (that is, the horizontal distance between the center points of two adjacent LED light source arrays is 1.2 times the distance between the light source and the silkworm cluster); a white frosted polycarbonate reflector with a diffuse reflectivity of 92% is set 0.4 meters below the light source.

[0010] Preferably, the shading treatment during the dark period adopts a three-layer composite shading structure: the inner layer is black nylon cloth, the middle layer is closed-cell foamed polyethylene (thickness 5mm), and the outer layer is silver reflective coated canvas; the gaps between doors and windows are pressed with magnetic sealing strips, and after shading, the illumination at any position in the silkworm room is ≤0.1lx.

[0011] Preferably, within 60 minutes after the dark period starts, air with an oxygen content of 21%-23% is introduced into the silkworm room at a rate of 100 m 3 The space is based on a flow rate of 0.5m 3 / min; the carbon dioxide concentration is monitored in real time by an infrared sensor. When the concentration exceeds 800ppm, the exhaust system is started to exhaust the gas and an equal amount of air is added at the same time.

[0012] Preferably, mating is completed within 2 hours after the silkworm moth emerges; the illumination of the mating environment is maintained at 500-600lx, the temperature is 26-27°C, and the relative humidity is 70%-72%; the mating time is controlled at 90-120 minutes, and individuals that have not separated within the time limit are artificially separated.

[0013] Preferably, the silkworm cluster adopts a double-layer composite structure: the inner layer is a bamboo mesh (porosity ≥ 80%), and the outer layer is covered with ramie cloth soaked in calcium hydroxide solution; before the dark period begins, quicklime particles with a particle size of 0.5-1mm are evenly spread on the floor of the silkworm room (dosage 15g / m²).

[0014] Preferably, the ramie cloth soaked in calcium hydroxide solution has a Ca(OH)2 concentration of 5%, a soaking time of 2 hours, and a pH value of 9.0-9.5 after drying.

[0015] Preferably, the surface of the cold white LED light source array is covered with a transparent ribbed polycarbonate plate (thickness 1.0±0.1mm), and the ribs are equilateral triangular protrusions with a depth of 0.2mm (side length 0.23-0.30mm); the walls of the silkworm room are painted with barium sulfate-based diffuse reflective paint.

[0016] The present invention has at least the following beneficial effects: The present invention regulates the photoperiod in coordination with temperature and humidity changes to simulate natural autumn cool signals to induce diapause, which is used for silkworm germplasm production, ensures the storability and planned production of silkworm seeds, and reduces resource waste.

[0017] The invention triggers the obvious generation of diapause eggs through the 8L:16D photoperiod, and the temperature is gradually adjusted (1°C / 10 minutes) to avoid the inhibition of neuroendocrine by sudden temperature change, which is beneficial to improving the diapause egg rate.

[0018] The present invention is beneficial to ensuring the quality of the dark period and the lighting effect through the shading and ultraviolet interference elimination methods and the uniform distribution of light sources, and effectively improves the diapause egg rate.

[0019] The present invention inhibits the germination of fungal spores (the optimal pH for Beauveria bassiana is 4.5-6.5) through the alkaline surface of ramie cloth, and the bamboo grid ensures air permeability; the quicklime on the ground absorbs condensed water and maintains a local low-humidity environment, which is beneficial for inhibiting fungi, reducing the risk of diseases during the high-humidity dark period, and synergistically improving the diapause egg rate.

[0020] The present invention is beneficial to improving the lighting effect through the refraction and transmission effects of the ribbed plate, and the barium sulfate paint forms an ultra-white reflective environment, eliminating the weak light area in the corner, which is beneficial to the lighting effect of the photoperiod and further improves the diapause egg rate.

[0021] Other advantages, objectives and features of the present invention will be reflected in part from the following description and will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of light source arrangement for one implementation of the method for increasing the diapause egg rate of silkworm germplasm resources according to the present invention. DETAILED DESCRIPTION

[0023] The present invention is further described in detail below with reference to the embodiments so that those skilled in the art can implement the invention with reference to the description.

[0024] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified. In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "set" should be understood in a broad sense, for example, they can be fixedly connected or set, or detachably connected or set, or integrally connected or set. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances. The orientations or positional relationships indicated by the terms "transverse", "longitudinal", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention.

[0025] The invention discloses a method for improving the diapause egg rate of silkworm germplasm resources. The method comprises: from the completion of pupation to the emergence of silkworm moths, adopting a light cycle of 8 hours of light: 16 hours of darkness (8L:16D) for regulation, wherein the light source does not contain an ultraviolet band; the light intensity is 350lx-600lx, and the light source is evenly distributed; the temperature is maintained at 26-28°C during the light period, and the temperature is reduced to 23-25°C during the dark period, thereby forming a day and night temperature difference; the temperature change is synchronously switched with the light cycle or gradually adjusted in stages; the relative humidity is 70-75% during the light period, and the relative humidity is increased to 75-85% during the dark period; and the humidity change is synchronously switched with the light cycle.

[0026] This method precisely regulates the reproductive rhythm of silkworms through photoperiods, synergizing diurnal temperature differences and humidity to simulate autumn coolness signals (inducing diapause). It also synergistically eliminates the interference of ultraviolet rays on the silkworm's physiological rhythm, creating a regular environmental rhythm, reducing environmental stress, and increasing the rate of diapause eggs. Furthermore, the selected temperature range is conducive to ensuring a low mortality rate, a high cocoon production rate, and a stable egg production.

[0027] Experiment 1 The light cycle is controlled from the fourth or fifth day of mature silkworms (when pupation is complete) to the emergence of silkworm moths, using a diurnal cycle of 8 hours light: 16 hours dark (8L:16D). Ventilation is maintained to avoid excessive vibration. Cool white LED light sources (350-600 lx) are used to ensure uniform light distribution and avoid UV interference. The light period simulates 8 hours of daylight to promote moth activity and mating. The dark period simulates 16 hours of night to stabilize hormone secretion.

[0028] Rearing temperature is a key parameter for germplasm conservation and research, and directly affects the maintenance of key production performance indicators, as shown in Table 1.

[0029] Table 1: Relationship between feeding temperature and key indicators As shown in Table 1, coordinated regulation of temperature and humidity is crucial: during the light period, the temperature is maintained at 26-28°C, with a relative humidity of 70-75%. During the dark period, the temperature is lowered to 23-25°C, creating a diurnal temperature swing, and the relative humidity is raised to 75-85%. Temperature and humidity changes are synchronized with the light cycle to create a regular environmental rhythm, preventing reproductive abnormalities caused by environmental stress. Maintaining a temperature range of 23-28°C is beneficial for ensuring low mortality rates, high cocoon production rates, and stable egg production.

[0030] Experiment 2 1. Experimental Design 1. Use natural light to simulate the duration of sunshine in different seasons, and use soda-lime glass window glass to filter out ultraviolet light and avoid ultraviolet interference.

[0031] 2. Make sure that only the duration of light exposure is a variable and other conditions (temperature, humidity, etc.) are completely consistent.

[0032] (1) During the light period, the temperature was maintained at 26°C and the relative humidity was maintained at 70-75%; during the dark period, the temperature dropped to 23°C, forming a day-night temperature difference, and the relative humidity increased to 75-85%; temperature changes were synchronized with the light cycle regulation, and humidity changes were synchronized with the light cycle regulation.

[0033] (2) Temperature: Use air conditioning to maintain stability.

[0034] (3) Humidity: Use a humidifier and dehumidifier to maintain stability.

[0035] 3. The experiment should be carried out in the same silkworm room or cocooning room to avoid the influence of latitude difference on sunlight intensity.

[0036] 2. Experimental steps: 1. Experimental groups: (1) Short-day group simulated winter (8L:16D): Shade 4 hours in advance every day. Use black cloth with 100% shading rate to cover the doors and windows of the silkworm room or cocooning room at regular intervals every day.

[0037] (2) Long-day group simulates summer (16L:8D): After the natural light period ends, lights with the same spectrum as natural light are used to supplement the sunlight for 16 hours.

[0038] (3) Full natural light group: After the natural light period ends, the group will use lights with the same spectrum as natural light to supplement the sunlight for 24 hours.

[0039] 2. Experimental operation: (1) Silkworm treatment: Hibiscus N and 932N were used. The light cycle was controlled from the 4th and 5th day of mature silkworms (when pupation was completed) to the emergence of silkworm moths. Each treatment consisted of 50 silkworms with 3 replicates.

[0040] (2) Record the actual duration of sunlight at a fixed time every day.

[0041] 3. After spawning, record the color and number of eggs, and count diapause eggs (dark brown) and non-diapause eggs (light yellow) separately.

[0042] Determination of diapause eggs: observe the egg color within 72 hours after spawning. Diapause egg rate = number of dark brown eggs / total number of eggs × 100%.

[0043] 3. Experimental results: Experiments were conducted on silkworms by artificially setting up a lighting environment with distinct day and night light. The experimental results showed that the diapause egg rate was higher in the short-day group and relatively lower in the long-day group. The experimental results of the light cycle and diapause egg rate are shown in Table 2.

[0044] Table 2: Relationship between photoperiod and diapause egg rate Example 1 A method for increasing the diapause egg rate of silkworm germplasm resources is basically the same as the short-day group simulated winter (8L:16D) method in Experiment 2, except that the temperature change and photoperiod are switched synchronously using a staged gradual adjustment. Specifically: During the period from the completion of pupation to the emergence of silkworms, a light cycle of 8 hours of light and 16 hours of darkness (8L:16D) is adopted for regulation, and the light source does not contain the ultraviolet band; the light intensity is 350lx-600lx, and the light source is evenly distributed; the temperature is maintained at 26°C during the light period and the temperature is reduced to 23°C during the dark period, forming a day and night temperature difference; the relative humidity is 70-75% during the light period and increased to 75-85% during the dark period; the temperature and humidity changes are synchronized with the light cycle and adopt a staged gradual adjustment: 30 minutes before the start of the light period, a step-by-step temperature increase program is initiated from 23°C to 26°C, with a step-by-step temperature increase rate of 1°C / 10 minutes; 30 minutes before the start of the dark period, a step-by-step temperature decrease program is initiated from 26°C to 23°C, with a step-by-step temperature decrease rate of 1°C / 10 minutes; temperature and humidity sensors are placed in the diagonal position and the center area of ​​the silkworm room, and the data are averaged as the execution basis.

[0045] Example 2 A method for increasing the diapause egg rate of silkworm germplasm resources is basically the same as Example 1, except that a cold white LED light source array is used as the light source, and a three-layer composite shading structure is used for the dark period shading treatment. Specifically: An array of cool white LED light sources is installed at a 45° angle on the ceiling of the silkworm chamber, with the spacing between the light sources 1.2 times the height of the illuminated surface (that is, the horizontal distance between the center points of adjacent LED light source arrays is 1.2 times the distance between the center points of the LED light source arrays and the silkworm cluster). A white frosted polycarbonate reflector with a diffuse reflectivity of 92% is placed 0.4 meters below the light source to effectively eliminate blind spots. During the dark period, a three-layer composite shading structure is used: an inner layer of black nylon cloth, a middle layer of closed-cell polyethylene foam (5mm thick), and an outer layer of silver reflective-coated canvas. Magnetic sealing strips seal the gaps between doors and windows. After shading, the illumination at any position in the silkworm chamber is ≤0.1lx, effectively eliminating light leakage interference.

[0046] Example 3 A method for increasing the diapause egg rate of silkworm germplasm resources is basically the same as Example 2, except that the carbon dioxide concentration is monitored in real time during the dark period and oxygen is supplemented, and the mating window period is controlled. Specifically: Within 60 minutes after the start of the dark period, air with an oxygen content of 21% was introduced into the silkworm room at a rate of 100 m 3 The space is based on a flow rate of 0.5m 3 / min; carbon dioxide concentration is monitored in real time by infrared sensors. When the concentration exceeds 800ppm, the exhaust system is activated to expel the gas while simultaneously replenishing an equal amount of air to effectively maintain respiratory metabolism during the dark period. Silkworms mate within 2 hours of eclosion; the mating environment is maintained at 500-600lx illumination, 26°C temperature, and 70%-72% relative humidity. Mating is controlled to 90-120 minutes, and individuals that fail to separate after the time limit are manually separated.

[0047] Example 4 A method for improving the diapause egg rate of silkworm germplasm resources is basically the same as Example 3, except that the silkworm cluster adopts a double-layer composite structure: the inner layer is a bamboo mesh (porosity ≥80%), and the outer layer is covered with ramie cloth soaked in calcium hydroxide solution (Ca(OH)2 concentration 5%, soaking time 2 hours, pH value 9.0-9.5 after drying); before the start of the dark period, quicklime particles with a particle size of 0.5-1 mm (dosage 15g / m²) are evenly spread on the floor of the silkworm room.

[0048] Example 5 A method for increasing the diapause egg rate of silkworm germplasm resources is basically the same as Example 4, except that a transparent ribbed polycarbonate sheet (thickness 1.0±0.1mm) is covered on the surface of the cold white LED light source array, and the ribs are equilateral triangular protrusions with a depth of 0.2mm (side length 0.23-0.30mm); and the walls of the silkworm room are painted with a barium sulfate-based diffuse reflective paint. When implemented, Figure 1As shown, the cold white light LED light source array 2 is installed at a 45° angle on the top 1 of the silkworm room. The four corners of the transparent ribbed polycarbonate plate 3 are provided with elastic claws, which are directly buckled into the reserved holes of the lamp panel of the cold white light LED light source array 2 to cover the surface of the lamp beads of the cold white light LED light source array 2. The horizontal distance between the center points of adjacent LED light source arrays is 1.2 times the distance between the light source and the silkworm cluster 5. A white frosted polycarbonate reflector 4 with a diffuse reflectivity of 92% is set 0.4 meters below the light source.

[0049] Experiment 3 1. Experimental steps: 1. Experimental grouping: designed according to the method of Examples 1-5.

[0050] 2. Experimental procedures for determining the rate of diapause eggs: (1) Silkworm treatment: Hibiscus N was used, and the light cycle was controlled from the 4th and 5th day of mature silkworms (when pupation was completed) to the emergence of silkworm moths. Each treatment consisted of 50 silkworms, with 3 replicates for each treatment.

[0051] (2) Record the actual duration of sunlight at a fixed time every day.

[0052] (3) After spawning, record the egg color and number, and count diapause eggs (dark brown) and non-diapause eggs (light yellow) separately.

[0053] Determination of diapause eggs: observe the egg color within 72 hours after spawning. Diapause egg rate = number of dark brown eggs / total number of eggs × 100%.

[0054] 3. Experimental procedures for determining the infection rate of Beauveria bassiana: (1) Sample collection: 1 hour before the end of the dark period, 30 fish were randomly selected from each group, with 3 replicates. The midgut and surface tissues (0.5 g) were aseptically excised and placed in a sterile bag.

[0055] (2) Fungal culture and identification: The sample was ground and suspended in 5 mL of sterile water, and then diluted to 10 -2 ; Inoculate into PDA medium (containing 0.05% chloramphenicol to inhibit bacteria); Culture conditions: Culture at 25℃ in the dark for 5-7 days (Beau. bassiana grows slowly).

[0056] (3) Confirmation of Beauveria bassiana: Observe the colony morphology: white fuzzy → gradually turns into light yellow powder (conidia); Microscopic examination: Spores were picked and stained with lactophenol cotton blue to confirm typical conidiophores and spherical conidia (2-3 μm in diameter). (4) Data calculation: Beauveria bassiana infection rate (%) = number of silkworms confirmed to be Beauveria bassiana positive by microscopic examination / total number of silkworms tested × 100%.

[0057] 2. Experimental results: The effects of the methods of different embodiments on the diapause egg rate and fungal infection are shown in Table 3.

[0058] Table 3: Effects of different examples on diapause egg rate and fungal infection The results in Tables 2 and 3 demonstrate that the present invention has industrial applicability and can be applied on a large scale to silkworm seed production, reducing resource waste. As shown in the short-day group and Example 1, the basic 8L:16D photoperiod significantly triggers the formation of diapause eggs. Gradual temperature regulation (1°C / 10 minutes) prevents sudden temperature changes from inhibiting neuroendocrine function and also helps increase the rate of diapause eggs. The shading method and UV interference elimination method, as well as the uniform distribution of light sources in Example 2, help ensure the quality and effectiveness of the dark period, significantly increasing the rate of diapause eggs. In Example 3, the introduction of 21%-23% oxygenated air during the dark period ensures efficient energy supply for the mitochondrial respiratory chain, promoting vitellogenin synthesis. Mating under an illumination of 500-600 lx activates PBAN neurons with light signals, ensuring synchronous sperm and egg maturation and preventing the formation of non-diapause eggs. In Example 4, ramie cloth (pH 9.0-9.5) is used to disrupt the optimal pH environment of Beauveria bassiana, effectively reducing infection rates without affecting the inhibition of non-diapause eggs, resulting in a dual-effect effect. Example 5 utilizes the refraction and transmission effects of the ribbed plate to improve the lighting effect, and the barium sulfate paint forms an ultra-white reflective environment, eliminating the weak light areas in the corners, which is beneficial to the lighting effect of the photoperiod and further improves the diapause egg rate.

[0059] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiment. They can be applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized.

Claims

1. A method for increasing the diapause egg rate of silkworm germplasm resources, characterized in that: During the period from the completion of pupation to the emergence of the silkworm moth, a light cycle of 8 hours of light and 16 hours of darkness is adopted for regulation, and the light source of the light does not contain the ultraviolet band; the light intensity is 350lx to 600lx, and the light source is evenly distributed; the temperature during the light period is maintained at 26-28°C, and the temperature during the dark period is reduced to 23-25°C, forming a day and night temperature difference; the temperature change is switched synchronously with the light cycle or adjusted gradually in stages; the relative humidity during the light period is 70-75%, and the relative humidity during the dark period is increased to 75-85%; the humidity change is switched synchronously with the light cycle.

2. The method according to claim 1, wherein The temperature switching between the light period and the dark period adopts a staged gradual adjustment; 30 minutes before the start of the light period, a step-by-step heating program is started to increase the temperature from 23-25°C to 26-28°C, with a step-by-step heating rate of 1°C / 10 minutes; 30 minutes before the start of the dark period, a step-by-step cooling program is started to decrease the temperature from 26-28°C to 23-25°C, with a step-by-step cooling rate of 1°C / 10 minutes; temperature and humidity sensors are respectively placed in the diagonal positions and the center area of ​​the silkworm room, and the data are averaged as the execution basis.

3. The method according to claim 1, wherein The uniform distribution of the light source is achieved by: a cold white LED light source array is installed on the top of the silkworm room at a 45° inclination angle, and the light source spacing is 1.2 times the height of the illuminated surface; a white frosted polycarbonate reflector with a diffuse reflectivity of 92% is set 0.4 meters below the light source.

4. The method according to claim 1, wherein The dark period shading treatment adopts a three-layer composite shading structure: the inner layer is black nylon cloth, the middle layer is closed-cell foamed polyethylene, and the outer layer is silver reflective coated canvas; the gaps between doors and windows are pressed with magnetic sealing strips. After shading, the illumination at any position in the silkworm room is ≤0.1lx.

5. The method according to claim 1, wherein Within 60 minutes after the dark period starts, air with an oxygen content of 21%-23% is introduced into the silkworm room. 3 The space is based on a flow rate of 0.5m 3 / min; the carbon dioxide concentration is monitored in real time by an infrared sensor. When the concentration exceeds 800ppm, the exhaust system is started to exhaust the gas and an equal amount of air is added at the same time.

6. The method according to claim 1, wherein Silkworms complete mating within 2 hours after emergence; the illumination of the mating environment is maintained at 500-600lx, the temperature is 26-27℃, and the relative humidity is 70%-72%; the mating time is controlled at 90-120 minutes, and individuals that fail to separate within the time limit are artificially separated.

7. The method according to any one of claims 1 to 6, wherein The silkworm cluster adopts a double-layer composite structure: the inner layer is a bamboo mesh, and the outer layer is covered with ramie cloth soaked in calcium hydroxide solution; before the dark period begins, quicklime particles with a particle size of 0.5-1mm are evenly spread on the floor of the silkworm room.

8. The method according to claim 7, wherein The ramie cloth soaked in calcium hydroxide solution has the following specific characteristics: Ca(OH)2 concentration of 5%, soaking time of 2 hours, and pH value of 9.0-9.5 after drying.

9. The method according to claim 8, wherein The surface of the cold white LED light source array is covered with a transparent ribbed polycarbonate plate, wherein the ribs are equilateral triangular protrusions with a depth of 0.2 mm; and the walls of the silkworm room are painted with a barium sulfate-based diffuse reflection paint.

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