Sixfold yeast composition and cosmetic containing same

Abstract

The invention discloses a sextuple yeast composition which comprises a bifidus yeast fermentation product lysate, a saccharomycetes / acetobacter xylinum / black tea fermentation product, a saccharomycetes / zinc fermentation product, galactose yeast-like bacterium fermentation product filtrate, a bifidus yeast fermentation product filtrate and a saccharomycetes / rice fermentation product filtrate in a mass ratio of 1: (1-1.2): (1-1.2): (4-6): (32-35): (160-168). According to the sextuple yeast composition disclosed by the invention, sextuple yeast fermentation products generate a synergistic effect, so that multiple effects of moisturizing, repairing, brightening, oil control, oxidation resistance and the like are realized, and the skin state is comprehensively improved. Long-term use can enhance skin barrier, reduce fine wrinkles, and improve skin gloss and elasticity.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a sextuple yeast composition and cosmetics containing the same. Background Art

[0002] As consumers' demand for skin care products has been upgraded to "multi-effects in one" and "precision repair", yeast fermentation products are increasingly widely used in moisturizing, anti-oxidation, barrier repair and other aspects due to their rich active ingredients such as amino acids, polysaccharides, and vitamins. However, the existing technologies have the following significant defects: (1) Single efficacy and insufficient synergy: Most skin care products containing yeast ingredients only use 1-2 yeast fermentation products, or simply compound a few ingredients, making it difficult to achieve the multi-dimensional synergistic effect of "moisturizing-repairing-anti-aging-brightening". For example, although a single bifid yeast product can repair the barrier, it lacks targeted oil control or long-term moisturizing ability; formulas containing only rice fermentation products are weak in anti-aging and firming. (2) The formula ratio is not scientific: The ingredient ratios of existing yeast compound products are mostly based on experience, and the optimal ratio has not been verified through gradient experiments, resulting in some active ingredients causing irritation due to excessive concentration, or weak efficacy due to insufficient concentration. For example, high concentrations of yeast lysates in some products may destroy the stability of the system, while low concentrations of antioxidant yeast ingredients cannot effectively scavenge free radicals. (3) The preparation process does not retain enough activity: conventional stirring or high-temperature treatment can easily lead to the inactivation of active peptides, polysaccharides and other ingredients in yeast fermentation products, reducing the actual efficacy of the product. At the same time, most formulas have not optimized the process for "multiple yeast compatibility", which is prone to stability problems such as stratification and precipitation. (4) Limited application scenarios: Existing products are difficult to take into account different skin types (such as the mildness of sensitive skin and the oil control needs of oily skin), or cannot meet the precise care needs of special scenarios (such as post-operative repair of medical aesthetics and emergency treatment of skin that stays up late).

[0003] Therefore, developing a skin care composition that achieves efficient synergistic efficacy and excellent stability by scientifically compounding multiple yeast fermentation products, optimizing formula ratios and preparation processes has become a technical problem that needs to be urgently solved in this field. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a six-fold yeast composition. The six-fold yeast fermentation products produce a synergistic effect to achieve multiple functions such as moisturizing, repairing, brightening, oil control, and anti-oxidation, thereby improving the skin condition in all directions. Long-term use can enhance the skin barrier, reduce fine lines, and enhance skin radiance and elasticity.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] The first aspect of the present invention provides a sextuple yeast composition, comprising a bifid yeast fermentation product lysate, a yeast / acetobacter xylinum / black tea fermentation product, a yeast / zinc fermentation product, a galactosidase-like bacteria fermentation product filtrate, a bifid yeast fermentation product filtrate and a yeast / rice fermentation product filtrate, with a mass ratio of 1:(1-1.2):(1-1.2):(4-6):(32-35):(160-168).

[0007] The six-fold yeast composition provided by the present invention comprises six-fold yeast fermentation products which can produce synergistic effects with each other, thereby achieving multiple functions such as moisturizing, repairing, brightening, oil control, and anti-oxidation.

[0008] As a core ingredient, yeast / rice fermentation product filtrate replenishes skin cells with nutrients such as amino acids, polysaccharides, and small peptides. Its polysaccharide components also form a preliminary moisturizing film, achieving a dual foundational effect of "nutrient supply and surface moisturization." This foundational effect paves the way for the efficacy of other yeast ingredients: for example, its amino acids enhance the absorption and utilization of subsequent ingredients (such as B vitamins from bifid yeast and active substances from galactosyl yeast) by skin cells. The surface moisturizing film reduces the penetration barriers of other ingredients caused by dry skin, indirectly enhancing the effectiveness of subsequent repair and anti-aging ingredients.

[0009] The filtrate of the bifid yeast ferment product focuses on barrier repair: by promoting the proliferation of stratum corneum cells and thickening the stratum corneum, it enhances the skin's resistance to external stimuli (UV rays, pollutants), while stimulating the synthesis of hyaluronic acid and strengthening endogenous moisturizing. The bifid yeast ferment product lysate focuses on deep repair and absorption promotion: its cytoplasmic contents (proteins, nucleic acids, etc.) directly replenish nutrients for skin cells, promote the repair of damaged cell DNA, and enhance the skin's absorption capacity for other ingredients. The two work together to form a closed loop of "surface barrier reinforcement + deep cell repair": the filtrate first strengthens the barrier to resist external damage and reduces the skin's "internal friction"; the lysate then deeply repairs damaged cells and helps the B vitamins, minerals and other ingredients in the filtrate penetrate more efficiently, while allowing the active substances of other yeast ingredients (such as galactosidase and black tea fermentation products) to break through the barrier and reach deep into the skin.

[0010] By accelerating the metabolism of dead skin cells and inhibiting oxidative stress, the galactosyl ferment filtrate improves skin clarity and reduces dullness and yellowing. Together with the yeast / rice ferment filtrate, which inhibits tyrosinase activity and hinders melanin synthesis, it creates a dual-pathway for brightening: the former reduces surface dullness by metabolizing dead skin cells, while the latter inhibits melanin at the source, reducing pigmentation. The two work together to achieve a faster and more lasting brightening effect. Furthermore, the galactosyl ferment filtrate regulates the balance of the skin's microbiome, inhibiting the reproduction of harmful bacteria and promoting the growth of beneficial bacteria. This, combined with the barrier-repairing properties of bifid yeast, creates a "stabilizing closed loop": barrier repair reduces damage to the microbiome caused by external stimuli, while microbial balance reduces sensitivity and redness caused by bacterial imbalance, providing a stable internal environment for the skin's other yeast ingredients (such as yeast / zinc ferment and black tea ferment).

[0011] The yeast / zinc ferment product specifically addresses excessive oil secretion and acne by regulating sebaceous gland secretion (inhibiting 5α-reductase activity) and providing antibacterial and anti-inflammatory properties (inhibiting Propionibacterium acnes), preventing oil accumulation from impacting the absorption of other ingredients (e.g., the oil film can hinder the penetration of polysaccharides and amino acids). Its damage-repairing properties (promoting wound repair and reducing acne scars) complement the deep-repairing properties of the bifid yeast lysate: the former focuses on inflammatory repair in acne-prone skin, while the latter focuses on overall cellular DNA repair. Together, these two products provide a seamless "oil control, anti-inflammatory, and repair" treatment for oily / acne-prone skin, while simultaneously controlling oil and preventing the weakened skin barrier caused by sole oil control.

[0012] The yeast / Acetobacter xylinum / black tea ferment product is rich in small-molecule tea polyphenols, which delay skin aging from an antioxidant perspective by scavenging free radicals and inhibiting lipid peroxidation. It also stimulates fibroblast proliferation and promotes collagen synthesis, achieving a dual anti-aging effect of "antioxidant + firming." This effect, combined with the anti-aging protection of the bifid yeast ferment product filtrate (which promotes collagen and elastin synthesis), forms a "dual anti-aging mechanism": the former reduces aging factors by scavenging free radicals, while the latter strengthens the skin's support through active collagen synthesis. Together, the two combat wrinkles and sagging from both "preventive" and "building" perspectives. Furthermore, the black tea ferment product, which promotes cellular energy metabolism, enhances the activity of small-molecule peptides in the yeast / rice ferment product filtrate, accelerates cellular metabolism, improves nutrient utilization efficiency, and indirectly strengthens the overall anti-aging effect.

[0013] The absorption-enhancing properties of the bifid yeast ferment lysate are the synergistic hub of the system: not only does it facilitate the penetration of its own nutrients, but it also enhances the absorption efficiency of the other five yeast ingredients—for example, allowing the brightening actives of galactosidase to reach the basal layer more quickly, the antioxidants of black tea ferment to more effectively target dermal fibroblasts, and the oil-controlling ingredients of the yeast / zinc ferment to more precisely target sebaceous gland cells. This global absorption-enhancing effect extends the benefits of the six yeasts beyond their individual areas of action, achieving layered coverage and global effectiveness through enhanced penetration efficiency.

[0014] In some preferred embodiments, in the sextuple yeast composition, the mass ratio of the bifid yeast fermentation product lysate, the yeast / acetobacter xylinum / black tea fermentation product, the yeast / zinc fermentation product, the galactosidase-like bacteria fermentation product filtrate, the bifid yeast fermentation product filtrate and the yeast / rice fermentation product filtrate is 1:(1-1.1):(1-1.2):(4-5):(30-35):(162-165).

[0015] In some preferred embodiments, in the sextuple yeast composition, the mass ratio of the bifid yeast fermentation product lysate, the yeast / acetobacter xylinum / black tea fermentation product, the yeast / zinc fermentation product, the galactosaccharomyces-like bacteria fermentation product filtrate, the bifid yeast fermentation product filtrate and the yeast / rice fermentation product filtrate is 1:1:1.1:4.5:33.7:164.0.

[0016] A second aspect of the present invention provides a cosmetic comprising the six-fold yeast composition;

[0017] Preferably, the cosmetics include facial masks, lotions, skin creams, toners, essences, moisturizing creams or lotions.

[0018] Furthermore, the added amount of the sextuple yeast composition is 5 to 95% of the total weight of the cosmetic, preferably 50 to 90%.

[0019] In some preferred embodiments, the cosmetic includes the following components by mass percentage: 88-90% of a sextuple yeast composition, 8-9% of butylene glycol, 0.6-0.7% of 1,2-hexanediol, 0.44-0.45% of parahydroxyacetophenone, 0.4-0.5% of glycerin, 0.15-0.17% of betaine, 0.12-0.13% of caprylyl glycol, 0.11-0.12% of 1,2-pentanediol, 0.02-0.04% of disodium EDTA, 0.01-0.03% of glyceryl polyacrylate, 0.0004-0.0006% of ethylhexylglycerin, and the balance of water.

[0020] In some preferred embodiments, the cosmetic comprises the following components by mass percentage: 89.35% of a sextuple yeast composition, 8.24% of butylene glycol, 0.69% of 1,2-hexanediol, 0.45% of parahydroxyacetophenone, 0.41% of glycerin, 0.16% of betaine, 0.13% of caprylyl glycol, 0.12% of 1,2-pentanediol, 0.03% of disodium EDTA, 0.02% of glyceryl polyacrylate, 0.0005% of ethylhexylglycerin, and the balance of water.

[0021] The third aspect of the present invention provides a method for preparing the cosmetic, comprising the following steps:

[0022] S1. Add water, butylene glycol and glycerin to the container, start stirring, then add the six-fold yeast composition and other ingredients, and continue stirring to mix the ingredients evenly;

[0023] S2. Detecting and adjusting the pH value of the mixture to 5.0-7.0 to obtain the cosmetic.

[0024] Furthermore, in step S1, a gradient speed stirring system is used for stirring, first stirring at a speed of 400-600 rpm for 5-10 minutes, and then adjusting the speed to 100-200 rpm and continuing stirring for 20-25 minutes.

[0025] In the present invention, a "gradient speed" is adopted in the stirring stage (initial 400-600 rpm dispersion, followed by gentle stirring at 100-200 rpm) to avoid high speed destruction of active ingredients (such as small molecule peptides) of yeast fermentation products and improve the activity retention rate of yeast fermentation products.

[0026] Furthermore, in step S1, after stirring evenly, a low-temperature homogenization treatment is also included, and the low-temperature homogenization treatment includes: first homogenizing at 15-20 MPa for 3-5 minutes, and then homogenizing at 25-30 MPa for 1-2 minutes; the temperature of the low-temperature homogenization is 20-42°C.

[0027] In the present invention, dynamic pressure regulation is performed during homogenization, first maintaining the pressure at 15-20 MPa for 3-5 minutes, and then maintaining the pressure at 25-30 MPa for 1-2 minutes. This can make the particle size distribution of the system more uniform and significantly improve the stability compared to homogenization under fixed pressure. In addition, homogenization at low temperature can reduce the damage of active ingredients (such as B vitamins) caused by high temperature.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. The six-fold yeast composition provided by the present invention forms a closed-loop efficacy system of "basic nutrition supply - barrier repair - anti-aging protection - oil control and brightening - deep absorption" through the precise compounding and synergistic effect of six yeast fermentation products.

[0030] 2. The cosmetic preparation method provided by the present invention utilizes a gradient stirring + low-temperature homogenization process, which significantly improves the retention rate of yeast active ingredients. After 6 months of accelerated testing (4°C, 25°C, and 40°C), the product showed no delamination or discoloration, and the active ingredient content decay rate was ≤5%, significantly superior to products prepared by conventional processes.

[0031] 3. The six-fold yeast composition provided by the present invention is suitable for dry, oily, combination and sensitive skin. Long-term use can improve fine lines, enhance skin elasticity, and meet anti-aging needs; short-term intensive care (such as in the form of a mask) can quickly relieve dull skin caused by staying up late and brighten the skin tone; and its low-irritation properties enable it to assist in barrier repair of the skin after medical aesthetics. DETAILED DESCRIPTION

[0032] The present invention will be further described below with reference to specific examples so that those skilled in the art can better understand the present invention and implement it, but the examples are not intended to limit the present invention.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art to which this invention pertains. The terms used in this specification are for the purpose of describing specific embodiments only and are not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0034] Unless otherwise specified, the experimental methods used in the following examples and comparative examples are conventional methods, and the materials and reagents used are all commercially available unless otherwise specified.

[0035] Example 1

[0036] This embodiment provides a sextuple yeast composition, comprising the following components by weight: 1 part of a bifid yeast fermentation product lysate, 1 part of a yeast / acetobacter xylinum / black tea fermentation product, 1.1 parts of a yeast / zinc fermentation product, 4.5 parts of a galactosidase-like yeast fermentation product filtrate, 33.7 parts of a bifid yeast fermentation product filtrate, and 164 parts of a yeast / rice fermentation product filtrate.

[0037] Example 2

[0038] This embodiment provides a sextuple yeast composition, comprising the following components in parts by weight: 1 part of a bifid yeast fermentation product lysate, 1.1 parts of a yeast / acetobacter xylinum / black tea fermentation product, 1.2 parts of a yeast / zinc fermentation product, 4 parts of a galactosidase-like yeast fermentation product filtrate, 35 parts of a bifid yeast fermentation product filtrate, and 160 parts of a yeast / rice fermentation product filtrate.

[0039] Example 3

[0040] This embodiment provides a sextuple yeast composition, comprising the following components by weight: 1 part of a bifid yeast fermentation product lysate, 1.1 parts of a yeast / acetobacter xylinum / black tea fermentation product, 1.1 parts of a yeast / zinc fermentation product, 5 parts of a galactosidase-like yeast fermentation product filtrate, 32 parts of a bifid yeast fermentation product filtrate, and 168 parts of a yeast / rice fermentation product filtrate.

[0041] Comparative Example 1

[0042] The difference between Comparative Example 1 and Example 1 is that the composition does not contain yeast / zinc fermentation product, and the yeast / rice fermentation product filtrate is increased by 1.1 parts.

[0043] Comparative Example 2

[0044] The difference between Comparative Example 2 and Example 1 is that the composition does not contain the bifid yeast fermentation product filtrate and the bifid yeast fermentation product lysate, and the yeast / rice fermentation product filtrate is increased by 34.7 parts.

[0045] Comparative Example 3

[0046] The difference between Comparative Example 3 and Example 1 is that the composition does not contain the filtrate of the fermentation product of galactosaccharomyces-like bacteria and the yeast / Acetobacter xylinum / black tea fermentation product, and the filtrate of the yeast / rice fermentation product is increased by 5.5 parts accordingly.

[0047] Comparative Example 4

[0048] The difference between Comparative Example 4 and Example 1 is that only the yeast / rice fermentation product filtrate is retained in the composition, and the amount thereof is 205.3 parts.

[0049] The formulations of Examples 1 to 3 and Comparative Examples 1 to 4 are shown in Table 1.

[0050] Table 1

[0051]

[0052] Application Examples

[0053] The compositions of Examples 1 to 3 and Comparative Examples 1 to 4 were applied to cosmetics to obtain skin care essences of Application Examples 1 to 3 and Comparative Application Examples 1 to 4, respectively. The specific formulas are shown in the following table:

[0054] Table 2

[0055]

[0056]

[0057] The preparation method of the above-mentioned skin care essence is:

[0058] S1. At room temperature, in a clean container equipped with a stirring device, water, butylene glycol, glycerin and other solvent components were added first, stirring was started, and then yeast fermentation products such as yeast / rice fermentation product filtrate and other ingredients were slowly added, stirred at 500 rpm for 5 minutes, adjusted to 200 rpm and stirred for 20 minutes to mix the ingredients evenly;

[0059] S2. After stirring, the mixture was homogenized at 20 MPa for 3 minutes and then at 30 MPa for 2 minutes;

[0060] S3. The mixed composition is tested for pH value, viscosity and other indicators, and the pH value is adjusted to 5.5 to obtain a skin care essence.

[0061] Performance testing

[0062] 240 volunteers aged 20-40 were selected and divided into 8 groups (blank control group and experimental group), with 30 people in each group, covering dry, oily, mixed and sensitive skin. Among them, the volunteers in the experimental group used the skin care essence of Application Examples 1 to 3 and Comparative Application Examples 1 to 4 respectively, and the volunteers in the blank control group did not use any skin care products. The specific method of use is: after the volunteers wash their faces with clean water, use the skin care essence once in the morning and evening for 28 consecutive days. The cheek skin water content, TEWL value, T-zone oil secretion and epidermal melanin content of the volunteers in the blank control group and the experimental group were tested on the 0th and 14th days, and the change rate of the above indicators was calculated. The results are shown in Table 3.

[0063] Among them, cheek skin water content is measured by The skin moisture tester is used to measure the TEWL value. The amount of oil secretion in the T zone was measured by the epidermal water loss tester. The oil tester was used to measure the epidermal melanin content. Melanin tester measurement.

[0064] Table 3

[0065]

[0066]

[0067] As shown in Table 3, the essence of the application example far exceeds any of the comparative application examples in terms of moisturizing, repairing, brightening, anti-oxidation, and oil control, proving that the comprehensive efficacy of the six-fold yeast composition of the present invention is not the result of the superposition of a single ingredient, but the result of synergistic enhancement.

[0068] Specifically, compared with the skin care essence of Application Example 1, which lacks the yeast / zinc fermentation product, its oil control effect drops sharply, proving that the yeast / zinc fermentation product synergistically enhances the oil control ability with other yeasts.

[0069] In contrast, the skin care essence of Application Example 2 lacks the filtrate of galactosidase-like bacteria fermentation product and the yeast / acetobacter xylinum / black tea fermentation product, and its brightening and antioxidant effects plummet, proving that the two together with the yeast / rice fermentation product filtrate, the bifid yeast fermentation product filtrate, and the bifid yeast fermentation product lysate synergistically achieve the dual effects of "brightening-anti-aging".

[0070] In contrast, the skin care essence in Application Example 3 lacks bifid yeast and lysate, and its barrier repair effect is almost lost, proving that it collaborates with other yeasts to build the core system of "barrier repair-deep repair".

[0071] The skin care essence of comparative application example 4 contains only yeast / rice fermentation product filtrate, and its various effects are the worst, proving that a single yeast cannot meet the needs of multi-effect skin care. The synergistic effect of six yeasts is the key to achieving efficient skin care.

[0072] In summary, the experimental data can directly prove that the six-fold yeast fermentation product forms a synergistic system through complementary effects and mechanism linkage, and its comprehensive skin care effect is significantly better than the formula lacking any one or more yeasts.

[0073] The above embodiments are merely preferred embodiments for the purpose of fully illustrating the present invention, and the scope of protection of the present invention is not limited thereto. Equivalent substitutions or modifications made by those skilled in the art based on the present invention are within the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

Claims

1. A sextuple yeast composition, characterized in that: The invention comprises bifid yeast fermentation product lysate, yeast / acetobacter xylinum / black tea fermentation product, yeast / zinc fermentation product, galactosaccharomyces-like bacteria fermentation product filtrate, bifid yeast fermentation product filtrate and yeast / rice fermentation product filtrate, with a mass ratio of 1:(1-1.2):(1-1.2):(4-6):(32-35):(160-168).

2. A six-fold yeast composition according to claim 1, characterized in that: In the six-fold yeast composition, the mass ratio of the bifid yeast fermentation product lysate, the yeast / acetobacter xylinum / black tea fermentation product, the yeast / zinc fermentation product, the galactosaccharomyces-like bacteria fermentation product filtrate, the bifid yeast fermentation product filtrate and the yeast / rice fermentation product filtrate is 1:(1-1.1):(1-1.2):(4-5):(30-35):(162-165).

3. The six-fold yeast composition according to claim 1, characterized in that: In the six-fold yeast composition, the mass ratio of the bifid yeast fermentation product lysate, the yeast / acetobacter xylinum / black tea fermentation product, the yeast / zinc fermentation product, the galactosaccharomyces-like bacteria fermentation product filtrate, the bifid yeast fermentation product filtrate and the yeast / rice fermentation product filtrate is 1:1:1.1:4.5:33.7:164.

0.

4. A cosmetic, characterized in that: The cosmetic comprises the six-fold yeast composition according to any one of claims 1 to 3; Preferably, the cosmetics include facial masks, lotions, skin creams, toners, essences, moisturizing creams or lotions.

5. A cosmetic according to claim 4, characterized in that: The added amount of the six-fold yeast composition is 5 to 95% of the total weight of the cosmetic, preferably 50 to 90%.

6. A cosmetic according to claim 4, characterized in that: The cosmetic comprises the following components by mass percentage: 88-90% of a sextuple yeast composition, 8-9% of butylene glycol, 0.6-0.7% of 1,2-hexanediol, 0.44-0.45% of p-hydroxyacetophenone, 0.4-0.5% of glycerin, 0.15-0.17% of betaine, 0.12-0.13% of caprylyl glycol, 0.11-0.12% of 1,2-pentanediol, 0.02-0.04% of disodium EDTA, 0.01-0.03% of glyceryl polyacrylate, 0.0004-0.0006% of ethylhexylglycerin, and the balance of water.

7. A cosmetic according to claim 6, characterized in that: The cosmetic includes the following components by mass percentage: 89.35% of a sextuple yeast composition, 8.24% of butylene glycol, 0.69% of 1,2-hexanediol, 0.45% of p-hydroxyacetophenone, 0.41% of glycerin, 0.16% of betaine, 0.13% of caprylyl glycol, 0.12% of 1,2-pentanediol, 0.03% of disodium EDTA, 0.02% of glyceryl polyacrylate, 0.0005% of ethylhexylglycerin, and the balance of water.

8. A method for preparing a cosmetic according to claim 6 or 7, characterized in that: The following steps are involved: S1. Add water, butylene glycol and glycerin to the container, start stirring, then add the six-fold yeast composition and other ingredients, and continue stirring to mix the ingredients evenly; S2. Detecting and adjusting the pH value of the mixture to 5.0-7.0 to obtain the cosmetic.

9. The method for preparing cosmetics according to claim 8, characterized in that: In step S1 , a gradient speed stirring system is used for stirring, first stirring at a speed of 400-600 rpm for 5-10 minutes, and then adjusting the speed to 100-200 rpm and stirring continuously for 20-25 minutes.

10. The method for preparing cosmetics according to claim 8, characterized in that: In step S1, after stirring evenly, low-temperature homogenization treatment is also included, and the low-temperature homogenization treatment includes: first homogenizing at 15-20 MPa for 3-5 minutes, and then homogenizing at 25-30 MPa for 1-2 minutes; the temperature of the low-temperature homogenization is 20-42°C.

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