Body-building beverage containing deer blood and preparation method of body-building beverage

Abstract

The invention relates to the technical field of health-care products, in particular to a deer blood-containing body-building beverage and a preparation method thereof. The body-building beverage containing deer blood comprises the following components in parts by mass: 10-20 parts of deer blood active peptide, 10-15 parts of poria cocos, 8-12 parts of Chinese yam, 6-8 parts of ginseng, 4-5 parts of lily, 1-4 parts of corn stigma, 10-18 parts of deer blood active peptide, 5-9 parts of grapefruit, 10-15 parts of black tea and 100-200 parts of water. According to the deer blood-containing body-building beverage disclosed by the invention, the monotonous taste of deer blood products is improved, the immunity-enhancing functional components of deer blood health-care products are remarkably improved, and the deer blood-containing body-building beverage is clear in health-care performance, convenient to digest and absorb by a human body, convenient to take without water, convenient to carry and suitable for market popularization.

Description

Technical Field

[0001] This application relates to the technical field of health products, and more specifically, to a health-enhancing beverage containing deer blood and its preparation method. Background Technology

[0002] The blood of the sika deer (Cervus nippon) and the red deer (Cervuse laphhus alashanicus) is the traditional and precious Chinese medicine, deer blood. Records of it can be found as early as the Ming Dynasty in Li Shizhen's *Compendium of Materia Medica*, and later in the late Qing Dynasty and early Republican era in Xu Ke's *Qing Dynasty Anecdotes*. In recent years, with increasing attention and emphasis on all-natural, side-effect-free, and novel health foods, people are paying more and more attention to the effects of deer blood health foods on beauty and skin care, treating anemia, regulating immunity, and delaying aging.

[0003] Immunity is the body's own defense mechanism, its ability to recognize and eliminate any invading foreign substances (viruses, bacteria, parasites, etc.) and to process its own cells. When the body's immunity is low, bacteria, viruses, mycoplasma, chlamydia, and fungi can easily become pathogens. People with low immunity are also prone to seasonal colds, chronic illnesses, and weakened abilities to clear aging cells, repair damaged cells, and monitor tumor cells, making them more susceptible to tumors. Long-term low immunity leads to poor mental state, weakness, and fatigue. Modern medicine indicates that 99% of human diseases are related to the immune system, making the research and development of products to enhance the body's immunity a crucial topic.

[0004] The medicinal and health benefits of deer blood are becoming increasingly well-known, leading to growing demand. Currently, deer blood products mainly include deer blood wine, deer blood crystals, deer blood tablets, and deer blood capsules. However, most of these preparations require water to take, which poses a challenge for people with alcohol allergies, difficulty swallowing, or limited access to water. Furthermore, the tableting process often involves excessive amounts of binders and other excipients, resulting in unclear health benefits, low disintegration, and slow absorption by the body. Most also have unpleasant tastes and adhere to the oral mucosa, causing discomfort and hindering the achievement of health benefits. The market lacks deer blood products that use deer blood as the primary ingredient, effectively enhance immunity, are of high quality, taste good, have high nutritional value, are convenient to carry, and offer higher added value. Summary of the Invention

[0005] In order to improve the monotonous taste of deer blood products, enhance the immune-boosting effects of deer blood health products, and facilitate human digestion and absorption, this application provides a health-strengthening beverage containing deer blood and its preparation method.

[0006] In the first aspect, this application provides a health-enhancing beverage containing deer blood, employing the following technical solution: A health-enhancing beverage containing deer blood comprises the following components in parts by weight: 10-20 parts deer blood active peptides, 10-15 parts Poria cocos, 6-8 parts ginseng, 8-12 parts yam, 4-5 parts lily bulb, 10-15 parts black tea, 1-4 parts corn silk, 5-9 parts grapefruit, 0.4-0.6 parts food additives, and 100-200 parts water.

[0007] By employing the above-mentioned technical solution, deer blood active peptides are organic compounds composed of multiple amino acids linked by peptide bonds. Peptide molecules are much smaller than protein molecules, facilitating human digestion and absorption, and exhibiting very high biological activity. Even small amounts of peptides can play crucial physiological roles, hence the name "active peptides." Deer blood active peptides can repair cells, improve cell metabolism, prevent cell degeneration, and promote cell growth and reproduction. They can also inhibit or kill various bacteria and fungi, as well as certain parasites, and even possess some killing activity against enveloped viruses and tumor cells. Furthermore, active peptides have a higher specificity in recognizing tumor cells with higher acidic phospholipid content in their outer membranes. Deer blood active peptides easily insert into cell lipid membranes to form ion channels, thereby destroying tumor cells. Deer blood active peptides undergo oxidative deamination in body tissues, generating corresponding α-keto acids that enter the tricarboxylic acid cycle for energy. Simultaneously, the removed amino groups couple with pyruvate or glutamate to form alanine and glutamic acid amide, providing energy and enhancing the body's immunity. Through the action of amino acids, they effectively remove and eliminate various metabolic wastes and toxins. Active peptides also possess strong antioxidant activity, scavenging oxygen free radicals in the body, promoting the oxygen-carrying capacity of erythrocytes, enhancing hematopoietic function, and preventing lipid oxidation, thus exhibiting broad-spectrum cell protection and immune-enhancing effects.

[0008] Poria cocos can invigorate the spleen and replenish qi, enhance immune function. A traditional Chinese medicine, it is mild in nature and has the effects of invigorating the spleen and replenishing qi, enhancing immunity, promoting diuresis and calming the mind, and reducing edema. It has a good therapeutic effect on patients with edema and difficulty urinating, and can treat symptoms such as abdominal distension, diarrhea, nausea, and vomiting caused by spleen deficiency and dampness. Because Poria cocos is rich in nutrients, it can relieve mental stress and contains polysaccharides, which can increase the body's immunity.

[0009] Yam is rich in nutrients such as potassium, dietary fiber, vitamin C, and B vitamins. It also contains a variety of active ingredients, including thymosin, a hormone secreted by the thymus gland. It is an immune enhancer that can promote the transformation of bone marrow hematopoietic stem cells into T cells, accelerate the maturation and differentiation of T cells, enhance the response of T cells to antigen stimulation, promote the production of cytokines by T cells, regulate the body's immune balance, improve the proliferation capacity of immune cells, strengthen the body's immune function, and also has anti-aging effects.

[0010] Ginseng is a traditional Chinese medicine widely used to regulate the immune system. It contains various bioactive components, such as ginsenosides and polysaccharides, which enhance immunity. Ginseng can promote the production of white blood cells, enhance the activity of immune cells, and regulate the balance of the immune system, making it more sensitive. Furthermore, ginseng can reduce the inflammatory response of the immune system, accelerate the repair of damaged tissues, and prevent the damage caused by leukopenia.

[0011] Grapefruit is high in vitamin C, which can boost immunity. It is also an important component in the production of dopamine, which has a refreshing and invigorating effect.

[0012] Corn silk contains various vitamins, sitosterols, flavonoids, pentosans, allantoin, etc., and has the effects of regulating immune function, anti-tumor, antibacterial and antioxidant, diuresis and prevention of urinary tract stone formation, lowering blood sugar and blood pressure, lowering lipids, as well as anti-aging, anti-cancer, lowering blood lipids and protecting the liver, and lowering blood sugar. When corn silk is combined with caffeine in black tea, it can stimulate the cerebral cortex and excite the central nervous system, thereby refreshing the mind, sharpening thinking and reaction, enhancing memory, and having an excitatory effect on the vascular system and heart, accelerating blood circulation to facilitate metabolism, having a growth stimulating effect, and enhancing non-specific immunity.

[0013] Lilies are rich in vitamins, which can promote skin cell metabolism, and can also clear the mind and relieve irritability, calm the nerves and soothe the nerves. They can be used for lingering fever after a febrile disease, mental confusion, insomnia and dreaminess, and are suitable for people with weak constitutions.

[0014] The addition of food additives helps prevent the deterioration of effective ingredients, ensures nutritional value, and extends shelf life.

[0015] The combination of the above ingredients creates a synergistic effect that enhances the body's immunity, resulting in significant therapeutic effects with a gentle approach. Its efficacy is significantly higher and more prolonged than when used alone, leading to unexpected benefits for deer blood health drinks in new prevention and treatment or health maintenance fields. Through experiments, the inventors have discovered that the composition of this invention has the effect of improving human immunity, and the beneficial effects on the human body after combining it in a certain proportion are unmatched by single-ingredient formulations.

[0016] In one specific feasible implementation, it also includes 5-18 parts by weight of Ganoderma lucidum extract.

[0017] By employing the above-mentioned technical solutions, the immune-enhancing effect of Ganoderma lucidum stems from its ability to improve the physiological functions of immune organs and cells, as well as the protective effect of Ganoderma lucidum polysaccharides on immune cells. Ganoderma lucidum enhances the body's immune organs, such as bone marrow, thymus, and spleen, in synthesizing DNA, RNA, and proteins. Abundance of DNA, RNA, and proteins enables the generation of a large number of new cells. Therefore, Ganoderma lucidum extract and deer blood active peptides have a certain synergistic immune effect. Deer blood active peptides destroy and metabolize tumor cells, while Ganoderma lucidum promotes the generation of new cells. Furthermore, because deamination of deer blood active peptides promotes coupling, forming alanine and glutamic acid amide, it provides energy to the body and enhances the defense capabilities of new cells against pathogens. Therefore, it can fundamentally enhance the antiviral capacity of cells without inducing drug resistance.

[0018] In one specific feasible implementation, the Ganoderma lucidum extract is prepared by the following method: S1: Raw material preparation: Ganoderma lucidum is pulverized to pass through a 30-50 mesh sieve, and hot water at 50-60℃ with a material-to-water ratio of 1:20-30 is added and mixed well; S2: Extraction of beneficial components: The raw material from step S1 is crushed and extracted. The extraction cycle time is 15-20 min, and then centrifuged to obtain centrifuged liquid. S3: Membrane filtration: The centrifuged liquid obtained in S2 is filtered at an operating pressure of 0.3-0.4 MPa and a temperature of 40-50℃ to obtain microfiltrate. The microfiltrate is then separated using a filter membrane with a specific molecular weight and vacuum dried at 60-75℃ for 2-3 hours to obtain Ganoderma lucidum extract.

[0019] By adopting the above technical solution and through a specific extraction process, the extraction purity of Ganoderma lucidum polysaccharides and triterpenes is significantly improved, preserving the active ingredients of Ganoderma lucidum. To a certain extent, the extract is decomposed into biomolecules, which is beneficial to the body's digestion and absorption, enabling the high-quality manufacturing of deer blood health products. In addition to polysaccharides and triterpenes, the Ganoderma lucidum extract, which enhances immunity, is also rich in protein, making it suitable for oral administration. It can have a good therapeutic effect on autoimmune diseases and can be used as an immunomodulator in combination with deer blood active peptides, having a strengthening effect. It can also significantly eliminate free radicals produced by the body, prevent free radical damage to the body, reduce fatigue, and improve sleep.

[0020] In one specific feasible implementation, the deer blood active peptide is prepared by the following method: (1) Raw material preparation: Centrifuge the blood of red deer or sika deer, take the supernatant plasma protein, mix plasma protein and acetone at a mass ratio of 1:50-100, precipitate for 1-2 hours, and centrifuge at differential speed to obtain turbid liquid; (2) Enzymatic hydrolysis; (1) Add buffer solution to the turbid liquid obtained in step 1 to adjust the pH to 6-9, and add proteolytic enzyme. The mass ratio of plasma protein to proteolytic enzyme is 300-500:1. Raise the temperature to 50-60℃ and enzymatically hydrolyze for 0.5-1.5h. Freeze dry to obtain deer blood active peptides and pulverize them to pass through a 60-80 mesh sieve.

[0021] By employing the above-mentioned technical solution, plasma proteins, composed of peptides, immunoproteins, and numerous trace elements, are immune-functional components of deer blood. Enzymatic hydrolysis preserves their inherent active functions. Targeted enzymatic digestion and specific separation techniques degrade large protein molecules into small peptides with good solubility and bioactivity. These small-molecule active peptides can promote antioxidant activity, lower blood pressure, enhance immunity, fight tumors, and promote mineral absorption. Centrifugation is used to first separate the immune-functional plasma proteins, allowing for the preparation of active peptides. The resulting products have higher purity and smaller molecular weight, facilitating full absorption by the body. Furthermore, all known and unknown active components of deer blood are preserved and enhanced. Compared to traditional direct consumption of whole deer blood, the functionally defined active components of deer blood are more stable.

[0022] In one specific implementation, the differential centrifugation in step (1) is carried out at 1000-10000 rpm for 30-60 minutes.

[0023] By adopting the above technical solution, plasma proteins with high protein content and good purity can be obtained by centrifuging deer blood, eliminating the tedious separation and purification steps; the centrifugation speed can affect the plasma protein extraction rate.

[0024] In one specific implementation, the proteolytic enzyme includes papain, pepsin, and figase, wherein the mass ratio of papain, pepsin, and figase is (2-3):1:(1-3).

[0025] By employing the above technical solution, a combination of proteolytic enzymes efficiently catalyzes the hydrolysis of proteins into small-molecule active peptides. Papain is a low-specificity proteolytic enzyme with high activity and good thermal stability, primarily catalyzing protein hydrolysis and preferentially breaking down peptide bonds of arginine and phenylalanine. Pepsin is a specific proteolytic enzyme, its main site of action being the peptide bonds composed of the amino groups of aromatic or acidic amino acids. Fig protease is a thiol protease with a high selenium content. Selenium is a protective agent against toxic substances, thus having functions such as delaying aging, enhancing immunity, and resisting disease. The use of these three proteolytic enzymes with different specific enzymatic fragments facilitates the rapid and efficient breakdown of complex and numerous protein groups in plasma proteins.

[0026] In one specific implementation, the food additive includes a sweetener, a flavoring, and a preservative. The flavoring is one or more of chocolate flavoring, vanilla flavoring, fruit flavoring, or bitterness masking flavoring. The sweetener includes one or two of mogroside and erythritol. The preservative is potassium sorbate. The ratio of the sweetener, flavoring, and preservative is 1:0.5-1:0.5-1.

[0027] By adopting the above technical solutions, sweeteners, flavorings and preservatives are combined to form the unique flavor of the beverage. The compounding can increase the viscosity to a certain extent, which helps to maintain and transfer the aroma.

[0028] In one specific implementation, the sweetener comprises mogroside and erythritol, wherein the mass ratio of mogroside to erythritol is 1:1.2-1.8.

[0029] By adopting the above technical solution, mogrosides, the main component of monk fruit, are a type of sugar substitute. When used in food, they can improve the taste and make the food more delicious. Sugar substitutes can produce sweetness without raising blood sugar levels. However, excessive consumption of mogrosides can lead to gastrointestinal disorders during decomposition. Erythritol, a sugar alcohol sweetener, has good tolerability and a small molecular weight. It can be rapidly absorbed in the cerebellum without being absorbed or digested, thus protecting gastrointestinal health. The combination of mogrosides and erythritol not only reduces the amount of each ingredient added, but also synergistically controls absorption and enhances flavor, resulting in a better sugar substitute effect.

[0030] Secondly, this application provides a method for preparing a health-enhancing beverage containing deer blood, using the following technical solution: A method for preparing a health-enhancing beverage containing deer blood includes the following steps: Add Poria cocos, yam, ginseng, lily bulb, corn silk, Ganoderma lucidum extract, deer blood active peptide, grapefruit, and black tea to water in sequence, heat to 90-100℃, maintain for 20-30 minutes, cool to room temperature, and filter through a 20-40 mesh filter cloth to obtain a mixture; add food additives to the above mixture, mix well, and obtain a health-strengthening beverage containing deer blood.

[0031] By adopting the above technical solution, water heating and the steam generated are used to fully extract the effective drug components of the material under normal pressure. Filtration reduces insoluble impurities in the beverage, ensuring the beverage's immune health benefits. The addition of food additives enhances the flavor of the beverage and ensures long-term storage, preventing the active ingredients from being contaminated by bacteria and fungi.

[0032] In summary, this application includes at least one of the following beneficial technical effects: 1. Extracting deer blood active peptides from deer blood plasma protein not only enriches the immune-active components of deer blood, but also removes the fishy smell of deer blood after enzymatic hydrolysis into smaller molecules, improving the taste of deer blood health products. Deer blood active peptides undergo oxidative deamination in body tissues, generating corresponding α-keto acids, which enter the tricarboxylic acid cycle for energy. Simultaneously, the removed amino groups couple with pyruvate or glutamate to form alanine and glutamic acid amide, providing energy and enhancing human immunity. Through the action of amino acids, various metabolic wastes and toxins are effectively removed and eliminated, thus exhibiting broad-spectrum cell protection and immune-enhancing effects. Combining deer blood active peptides with Poria cocos, yam, ginseng, lily bulb, corn silk, Ganoderma lucidum extract, grapefruit, black tea, and food additives to create a beverage can promote digestion and absorption, effectively enhancing human immunity.

[0033] 2. Ganoderma lucidum can enhance the physiological functions of immune organs and immune cells, and Ganoderma lucidum polysaccharides can protect immune cells. Ganoderma lucidum can improve the body's immune organs, such as bone marrow, thymus, and spleen, in their ability to synthesize DNA, RNA, and proteins. DNA, RNA, and proteins are all essential energy substances for new cells. Therefore, Ganoderma lucidum extract and deer blood active peptides have a certain synergistic immune effect. Deer blood active peptides destroy and metabolize tumor cells, while Ganoderma lucidum promotes the generation of new cells. Furthermore, because deamination of deer blood active peptides promotes coupling, it enhances the defense capabilities of new cells against pathogens, fundamentally enhancing the antiviral capabilities of cells without inducing drug resistance.

[0034] 3. By using a combination of mogrosides and erythritol, mogrosides, the main component of monk fruit, are a type of sugar substitute. When used in food, they can improve the taste and make the food more delicious. While sugar substitutes produce sweetness, they do not raise blood sugar levels. However, excessive consumption of mogrosides can lead to gastrointestinal disorders during their breakdown. Erythritol, a sugar alcohol sweetener, is well-tolerated, has a small molecular weight, and is rapidly absorbed in the cerebellum without being digested, thus protecting gastrointestinal health. The combination of mogrosides and erythritol not only reduces the amount of each ingredient added but also synergistically controls absorption and enhances flavor, resulting in a richer taste and a better sugar substitute effect. Detailed Implementation

[0035] The present application will be further described in detail below with reference to the embodiments.

[0036] Poria cocos powder and yam concentrate powder were purchased from Baoji Liupanyun Biotechnology Co., Ltd.; yam concentrate powder, ginseng extract, grapefruit extract, and black tea powder were purchased from Waterles Biotechnology Co., Ltd.; lily concentrate powder was purchased from Xi'an Tongjiang Biotechnology Co., Ltd.; and corn silk extract was purchased from Nanjing Tongying Biotechnology Co., Ltd.

[0037] Unless otherwise specified, all raw materials used in the examples and comparative examples are commercially available products.

[0038] Preparation Example Preparation of deer blood bioactive peptides: Preparation Example 1 The blood of red deer or sika deer was centrifuged at 3000 rpm for 10 minutes, and the supernatant plasma protein was collected. The plasma protein and acetone were mixed at a mass ratio of 1:50, and the mixture was allowed to precipitate for 1 hour. Then, it was centrifuged at 1000 rpm for 60 minutes to obtain a turbid liquid. Citric acid was added to the turbid liquid to adjust the pH to 6. Proteolytic enzymes, including papain, pepsin, and figase, were added to the turbid liquid at a mass ratio of 1:300 (papain, pepsin, and figase in a mass ratio of 2:1:1). The temperature was raised to 50°C, and the enzymes were enzymatically hydrolyzed for 0.5 hours. The precipitated liquid was freeze-dried to obtain deer blood active peptides, which were then pulverized to pass through a 60-mesh sieve.

[0039] Preparation Example 2 Blood from red deer or sika deer was centrifuged at 3000 rpm for 10 minutes, and the supernatant plasma protein was collected. The plasma protein and acetone were mixed at a mass ratio of 1:100, and the mixture was allowed to precipitate for 1 hour. Then, it was centrifuged at 1000 rpm for 60 minutes to obtain a turbid liquid. Citric acid was added to the turbid liquid to adjust the pH to 6. Proteolytic enzymes, including papain, pepsin, and figase, were added to the turbid liquid at a mass ratio of 1:300 (papain, pepsin, and figase in a mass ratio of 2:1:1). The temperature was raised to 50°C, and the enzymes were enzymatically hydrolyzed for 0.5 hours. The precipitated liquid was freeze-dried to obtain deer blood active peptides, which were then pulverized to pass through a 60-mesh sieve.

[0040] Preparation Example 3 Blood from red deer or sika deer was centrifuged at 3000 rpm for 10 minutes, and the supernatant plasma protein was collected. The plasma protein and acetone were mixed at a mass ratio of 1:100, and the mixture was allowed to precipitate for 2 hours. Then, it was centrifuged at 10000 rpm for 30 minutes to obtain a turbid liquid. Next, a buffer solution was added to the obtained turbid liquid to adjust the pH to 9. Proteolytic enzymes, including papain, pepsin, and figase, were added to the turbid liquid at a mass ratio of 1:500 (papain, pepsin, and figase in a mass ratio of 2:1:2). The temperature was raised to 60°C, and the enzymatic hydrolysis was carried out for 1.5 hours. The mixture was then freeze-dried to obtain deer blood active peptides, which were then pulverized to pass through an 80-mesh sieve.

[0041] Preparation Example 4 Blood from red deer or sika deer was centrifuged at 3000 rpm for 10 minutes, and the supernatant plasma protein was collected. The plasma protein and acetone were mixed at a mass ratio of 1:100, and the mixture was allowed to precipitate for 2 hours. Then, it was centrifuged at 10000 rpm for 30 minutes to obtain a turbid liquid. Next, a buffer solution was added to the obtained turbid liquid to adjust the pH to 9. Proteolytic enzymes, including papain, pepsin, and figase, were added to the turbid liquid at a mass ratio of 1:500 (papain, pepsin, and figase in a mass ratio of 3:1:3). The temperature was raised to 60°C, and the enzymatic hydrolysis was carried out for 1.5 hours. The mixture was then freeze-dried to obtain deer blood active peptides, which were then pulverized to pass through an 80-mesh sieve.

[0042] Preparation Example 5 Blood from red deer or sika deer was centrifuged at 3000 rpm for 10 minutes, and the supernatant plasma protein was collected. The plasma protein and acetone were mixed at a mass ratio of 1:100, and the mixture was allowed to precipitate for 2 hours. The mixture was then centrifuged at 10000 rpm for 30 minutes to obtain a turbid liquid. Buffer solution was added to the turbid liquid to adjust the pH to 9. Proteolytic enzymes, including papain, pepsin, and figase, were added to the turbid liquid at a mass ratio of 1:500 (papain, pepsin, and figase in a mass ratio of 1:1:3). The temperature was raised to 60°C, and enzymatic hydrolysis was performed for 1.5 hours. The mixture was then freeze-dried to obtain deer blood active peptides, which were then pulverized to pass through an 80-mesh sieve.

[0043] Preparation Example 6 Blood from red deer or sika deer was centrifuged at 3000 rpm for 10 minutes, and the supernatant plasma protein was collected. The plasma protein and acetone were mixed at a mass ratio of 1:100, and the mixture was allowed to precipitate for 2 hours. Then, it was centrifuged at 10000 rpm for 30 minutes to obtain a turbid liquid. Next, a buffer solution was added to the obtained turbid liquid to adjust the pH to 9. Proteolytic enzymes, including papain, pepsin, and figase, were added to the turbid liquid at a mass ratio of 1:500 (papain, pepsin, and figase in a mass ratio of 1:1:1). The temperature was raised to 60°C, and the enzymes were enzymatically hydrolyzed for 1.5 hours. The mixture was then freeze-dried to obtain deer blood active peptides, which were then pulverized to pass through an 80-mesh sieve.

[0044] Preparation Example 7 Centrifuge the blood of red deer or sika deer at 3000 rpm for 10 minutes, collect the supernatant plasma protein, add acetone at a 1:1 mass ratio, precipitate for 2 hours, centrifuge at 10000 rpm for 30 minutes to obtain a turbid liquid; then add buffer solution to the obtained turbid liquid to adjust the pH to 9, and add papain according to the mass ratio of papain to plasma protein of 1:500, raise the temperature to 60℃, enzymatically hydrolyze for 1.5 hours, freeze dry to obtain deer blood active peptides, and pulverize to the point that they can pass through an 80-mesh sieve.

[0045] Preparation Example 8 Centrifuge the blood of red deer or sika deer at 3000 rpm for 10 minutes, collect the supernatant plasma protein, add acetone at a 1:1 mass ratio, precipitate for 2 hours, centrifuge at 10000 rpm for 30 minutes to obtain a turbid liquid; then add buffer solution to the obtained turbid liquid to adjust the pH to 9, and add pepsin according to the mass ratio of pepsin to plasma protein of 1:500, raise the temperature to 60℃, enzymatically hydrolyze for 1.5 hours, freeze dry to obtain deer blood active peptides, and pulverize to the point that they can pass through an 80-mesh sieve.

[0046] Preparation Example 9 Centrifuge the blood of red deer or sika deer at 3000 rpm for 10 minutes, collect the supernatant plasma protein, add acetone at a 1:1 mass ratio, precipitate for 2 hours, centrifuge at 10000 rpm for 30 minutes to obtain a turbid liquid; then add buffer solution to the obtained turbid liquid to adjust the pH to 9, and add fig enzyme according to the mass ratio of fig enzyme to plasma protein 1:500, raise the temperature to 60℃, enzymatically hydrolyze for 1.5 hours, freeze dry to obtain deer blood active peptides, and pulverize to the point that they can pass through an 80-mesh sieve.

[0047] Preparation Example 10 Blood from red deer or sika deer was centrifuged at 3000 rpm for 10 minutes, and the supernatant plasma protein was collected. The plasma protein and acetone were mixed at a mass ratio of 1:100, and the mixture was allowed to precipitate for 2 hours. Then, it was centrifuged at 10000 rpm for 30 minutes to obtain a turbid liquid. Next, a buffer solution was added to the obtained turbid liquid to adjust the pH to 9. Proteolytic enzymes, including papain, pepsin, and figase, were added to the turbid liquid at a mass ratio of 1:500 (papain, pepsin, and figase in a mass ratio of 2:1:1). The temperature was raised to 60°C, and the enzymatic hydrolysis was carried out for 1.5 hours. The mixture was then freeze-dried to obtain deer blood active peptides, which were then pulverized to pass through an 80-mesh sieve.

[0048] Preparation of Ganoderma lucidum extract: Preparation Example 11 Ganoderma lucidum was pulverized to pass through a 30-mesh sieve and then mixed with 50°C hot water at a material-to-water ratio of 1:20. The mixture was then fed into an extraction and cell-wall breaking unit for extraction. The extraction cycle time was 15 minutes, followed by centrifugation to obtain the centrifuged liquid. The obtained centrifuged liquid was filtered through a ceramic membrane at an operating pressure of 0.3 MPa and a temperature of 40°C to obtain the microfiltrate. A nanofiltration membrane with a molecular weight of 1000 was used for separation to obtain a concentrate of beneficial components, mainly Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenes. The extraction residue was discarded, and the concentrate was vacuum dried at 60°C for 2 hours to obtain the Ganoderma lucidum extract. Preparation Example 12 Ganoderma lucidum was pulverized to pass through a 50-mesh sieve and then mixed with hot water at 60°C at a material-to-water ratio of 1:30. The mixture was then fed into an extraction and cell-wall breaking unit for extraction. The extraction cycle time was 20 minutes, followed by centrifugation to obtain the centrifuged liquid. The obtained centrifuged liquid was filtered through a ceramic membrane at an operating pressure of 0.4 MPa and a temperature of 50°C to obtain the microfiltrate. A nanofiltration membrane with a molecular weight of 1100 was used for separation to obtain a concentrate of beneficial components, mainly Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenes. The extraction residue was discarded, and the concentrate was vacuum dried at 75°C for 3 hours to obtain the Ganoderma lucidum extract. Example

[0049] Example 1 A health-enhancing beverage containing deer blood includes 10g of Poria cocos, 8g of yam, 6g of ginseng, 4g of lily bulb, 1g of corn silk, 10g of deer blood active peptides, 5g of grapefruit, 10g of black tea, 0.4g of food additives, and 100g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.2.

[0050] The preparation method of the health-strengthening beverage containing deer blood is as follows: Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptides obtained in Preparation Example 2, grapefruit, and black tea are added to water in sequence, stirred and heated to 90°C, maintained for 20 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, chocolate flavoring, and potassium sorbate are added to the filtrate in sequence, mixed and homogenized, pasteurized, and bottled.

[0051] Example 2 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.4g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.2.

[0052] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0053] Example 3 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.4g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5, and the sweetener is mogroside.

[0054] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0055] Example 4 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.4g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5, and the sweetener is erythritol.

[0056] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 90°C, maintained for 20 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and erythritol, vanilla flavoring and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0057] Example 5 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.4g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0058] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0059] Example 6 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.4g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.

[0060] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0061] Example 7 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.4g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:2.5.

[0062] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0063] Example 8 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.2.

[0064] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0065] Example 9 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0066] Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0067] Example 10 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 5g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0068] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 11, deer blood active peptide prepared in Preparation Example 2, Ganoderma lucidum extract prepared in Preparation Example 11, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0069] Example 11 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 5g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0070] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0071] Example 12 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0072] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0073] Example 13 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 15g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0074] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0075] Example 14 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0076] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 1, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0077] Example 15 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0078] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 3, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0079] Example 16 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0080] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 4, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0081] Example 17 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0082] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 5, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0083] Example 18 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0084] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 6, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0085] Example 19 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0086] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 7, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0087] Example 20 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0088] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 8, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0089] Example 21 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0090] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 9, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0091] Example 22 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 18g of Ganoderma lucidum extract, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0092] Poria cocos, yam, ginseng, lily, corn silk, Ganoderma lucidum extract prepared in Preparation Example 12, deer blood active peptide prepared in Preparation Example 10, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, and then mogroside, erythritol, vanilla flavoring, and potassium sorbate were added to the filtrate in sequence, mixed and homogenized, pasteurized, and filled.

[0093] Comparative Example Comparative Example 1 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0094] Add Poria cocos, yam, ginseng, lily bulb, corn silk, grapefruit, and black tea to water, stir and heat to 100℃, maintain for 30 minutes, cool to room temperature, filter through a 20-mesh filter cloth, add mogroside, erythritol, vanilla flavoring and potassium sorbate to the filtrate in sequence, mix and homogenize, pasteurize, and fill.

[0095] Comparative Example 2 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of freeze-dried deer blood powder, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. The food additives are a mixture of sweetener, chocolate flavoring, and potassium sorbate in a mass ratio of 1:0.5:0.5. The sweeteners are a mixture of mogroside and erythritol in a mass ratio of 1:1.8.

[0096] Add Poria cocos, yam, ginseng, lily bulb, corn silk, freeze-dried deer blood powder, grapefruit, and black tea to water, stir and heat to 100℃, maintain for 30 minutes, cool to room temperature, filter through a 20-mesh filter cloth, add mogroside, erythritol, vanilla flavoring and potassium sorbate to the filtrate in sequence, mix and homogenize, pasteurize, and fill.

[0097] Comparative Example 3 A health-enhancing beverage containing deer blood includes 15g of Poria cocos, 12g of yam, 8g of ginseng, 5g of lily bulb, 4g of corn silk, 20g of deer blood active peptides, 9g of grapefruit, 15g of black tea, 0.6g of food additives, and 200g of water. Poria cocos, yam, ginseng, lily, corn silk, deer blood active peptide prepared in Example 2, grapefruit, and black tea were added to water, stirred and heated to 100°C, maintained for 30 minutes, cooled to room temperature, filtered through a 20-mesh filter cloth, pasteurized, and filled.

[0098] Performance testing As this application pertains to a health supplement, it was tested in accordance with the "Technical Specifications for Inspection and Evaluation of Health Foods." The testing items included: acute toxicity test, Ames test, and mouse bone marrow cell micronucleus test. The test results and judgment opinions are as follows: 1. Acute toxicity test: MTD > 1000 mL / kg BW (equivalent to 60 mL / kg BW of concentrated sample solution, or 600 times the recommended daily intake), classifying it as non-toxic according to acute toxicity classification. 2. Ames test: Ames test result was negative. 3. Mouse bone marrow cell micronucleus test: Mouse bone marrow polychromatic erythrocyte micronucleus test result was negative.

[0099] The MTT assay was used to conduct ConA-induced transformation of mouse spleen lymphocytes, in accordance with the Technical Specifications for Inspection and Evaluation of Health Foods.

[0100] Experimental animals: SPF-grade KM mice, weighing 15-17g, 15 mice per group.

[0101] Test samples: Health drinks containing deer blood from Examples 1-22 and Comparative Examples 1-3.

[0102] Dosage: Mice were administered 2 mL / g bw via gavage once daily for 30 days. An equal volume of distilled water was used as a blank control group.

[0103] Experimental procedure: 30 minutes after the last administration of the test sample, the mice were euthanized by cervical dislocation, and the spleen was aseptically removed. The remaining steps were in accordance with the "Technical Specifications for Inspection and Evaluation of Health Food". Lymphocyte proliferation capacity: the optical density value (OD value) of the ConA well was compared with the OD value of the control well. The results are shown in Table 1. Different letters indicate that there is a significant difference between the two groups (P<0.05).

[0104] Table 1: Immunity Test This application describes a health-enhancing beverage containing deer blood. The evaluation was conducted using common food sensory evaluation methods. The calculation method for the sensory evaluation values ​​was based on the evaluation methods in "Food Sensory Evaluation" published by Chemical Industry Press. The complete removal of fishy odor was set at 100. For specific calculations, please refer to Table 2: Product Experimental Evaluation Standards. The sensory evaluation values ​​of the health-enhancing drinks containing deer blood in Examples 1-22 and Comparative Examples 1-3 were compared, and the results are shown in Table 3: Table 3: Comparison of Sensory Evaluation Values ​​of Health-Boosting Drinks Containing Deer Blood Referring to Table 1, comparing the health-enhancing beverages containing deer blood in Examples 8-22 and Comparative Examples 1-2, immune system tests were conducted. The proliferation capacity of lymphocytes in mice in the experimental and control groups was examined. The results showed that, compared to the control group, mice administered the health-enhancing beverage containing deer blood via gavage daily at 2 mL / g·bw exhibited a significant increase in lymphocyte proliferation capacity, indicating that deer blood active peptides significantly enhance the body's immunity. In contrast, the deer blood freeze-dried powder in Comparative Example 2 contained large molecular weight immune-enhancing active substances, which were not easily digested and absorbed by the body, resulting in less than ideal health benefits. Furthermore, comparing Examples 8-9 with Examples 10-12 revealed that Ganoderma lucidum extract also has immunomodulatory effects. Ganoderma lucidum extract and deer blood active peptides exhibit a certain synergistic immune effect, promoting rapid proliferation of lymphocytes and enhancing the defense capabilities of new cells against pathogens, thereby fundamentally strengthening the body's immune function. Comparing Examples 15-16 and Examples 17-22, the organic combination of proteolytic enzymes with different specific cleavage sites is beneficial for enzymatically hydrolyzing large polypeptides into small molecule active peptides with higher activity, which is conducive to full absorption by the body and enhances immune regulation.

[0105] Referring to Tables 2-3, comparing Examples 8-22 and Comparative Examples 1-3, the degree of enzymatic hydrolysis of deer blood plasma proteins significantly affects the sensory evaluation results of the health-enhancing beverages containing deer blood. Compared to Examples 17-21, Examples 15-16 and 22 show more thorough plasma protein hydrolysis, resulting in a clearer product appearance. Comparing Examples 5, 6-7, and 3-4, the combination of mogrosides and erythritol not only reduces the amount of single-agent additives but also synergistically controls absorption and enhances flavor, resulting in a better sugar-substituting effect. Comparing Examples 1-22 with Comparative Example 3, it is evident that food additives can effectively improve the taste and flavor of products. Health-enhancing beverages containing deer blood not only effectively boost immunity but also offer a rich and unique flavor, increasing product added value and facilitating market promotion.

[0106] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A tonic beverage containing deer blood, characterized in that, The components include the following components by mass: 10-20 parts of deer blood active peptide, 10-15 parts of poria cocos, 6-8 parts of ginseng, 8-12 parts of yam, 4-5 parts of lily, 10-15 parts of black tea, 1-4 parts of corn silk, 5-9 parts of grapefruit, 0.4-0.6 parts of food additive, and 100-200 parts of water.

2. The body-strengthening beverage containing deer blood according to claim 1, characterized in that, It also includes 5-18 parts of ganoderma extract by mass.

3. The body-strengthening beverage containing deer blood according to claim 2, characterized in that, The ganoderma extract is prepared by the following method: S1: raw material preparation: the ganoderma is crushed to pass through a 30-50 mesh screen, and 50-60℃ hot water is added at a material to water ratio of 1:20-30, and mixed uniformly; S2: extraction of beneficial components: the raw material of step S1 is crushed and extracted, the extraction cycle time is 15-20 min, and then centrifuged to obtain a centrifugal liquid; S3: membrane filtration: the centrifugal liquid obtained in step S2 is filtered, the operation pressure is 0.3-0.4 MPa, and the temperature is 40-50℃ to obtain a microfiltrate, and the obtained microfiltrate is separated by a specific molecular weight filter membrane, vacuum dried at 60-75℃ for 2-3h to obtain the ganoderma extract.

4. The body-strengthening beverage containing deer blood according to claim 1, characterized in that, The deer blood active peptide is prepared by the following method: (1) raw material preparation: the blood of the elk or the sika deer is centrifuged, and the supernatant plasma protein is taken, the plasma protein and acetone are mixed at a mass ratio of 1:50-100, precipitated for 1-2 hours, and differentially centrifuged to obtain a turbid liquid; (2) enzymolysis: the turbid liquid obtained in step (1) is added with a buffer solution to adjust the pH to 6-9, and a proteolytic enzyme is added, the mass ratio of the plasma protein to the proteolytic enzyme is 300-500:1, the temperature is raised to 50-60℃, and the enzymolysis is carried out for 0.5-1.5h, and then freeze-dried to obtain the deer blood active peptide, which is crushed to pass through a 60-80 mesh screen.

5. The body-strengthening beverage containing deer blood according to claim 4, characterized in that, The differential centrifugation is carried out at 1000-10000 rpm for 30-60 minutes.

6. The body-strengthening beverage containing deer blood according to claim 4, characterized in that, The proteolytic enzyme includes papain, pepsin, and ficin, and the mass ratio of the papain, pepsin, and ficin is (2-3):1:(1-3).

7. The body-strengthening beverage containing deer blood according to claim 1, wherein the beverage is a beverage for health care. The food additive includes a sweetener, an essence, and a preservative, the essence is one or more of chocolate essence, vanilla essence, fruit flavor essence, or bitter taste masking essence, the sweetener includes one or both of mogroside and erythritol, and the preservative is potassium sorbate, and the mass ratio of the sweetener, the essence, and the preservative is 1:0.5-1:0.5-1.

8. The body-strengthening beverage containing deer blood according to claim 7, characterized in that, The sweetener includes mogroside and erythritol, and the mass ratio of the mogroside to the erythritol is 1:1.2-1.

8.

9. The method of claim 1-8, wherein the method of preparing the deer blood-containing health drink is characterized by, The steps include: The poria cocos, yam, ginseng, lily, corn silk, ganoderma extract, deer blood active peptide, grapefruit, and black tea are sequentially added into water, heated to 90-100℃, maintained for 20-30 minutes, and then cooled to room temperature to obtain a mixed liquid; The food additive is added into the above mixed liquid, and mixed uniformly to obtain a strong body drink containing deer blood.

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