Rapid humification method for enteromorpha prolifera and application

Abstract

The invention belongs to the technical field of environmental science and resource utilization, and relates to a rapid enteromorpha humification method and application, under the action of a catalyst, enteromorpha is humified by a hydrothermal method to obtain an enteromorpha-based bio-organic fertilizer; the catalyst is more than one of nano ferric oxide, nano ferric chloride, nano cuprous chloride and ammonium sulfite. According to the method, rapid humification of the enteromorpha is realized, the high-activity fertilizer is prepared, the pollution problem of the enteromorpha is effectively solved, and resource utilization of the enteromorpha is promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of environmental science and resource utilization, and relates to a method for rapid humification of enteromorpha and its application. Background Art

[0002] In recent years, frequent green tides caused by drifting Enteromorpha have occurred in my country's Yellow Sea, posing a serious threat to the marine ecosystem. The rapid spread of Enteromorpha can severely impact seawater quality and tourism, requiring significant human, material, and financial resources to salvage and dispose of the disease. Therefore, effectively controlling Enteromorpha pollution and repurposing it as a resource has become a crucial issue in improving the marine ecosystem.

[0003] Enteromorpha is rich in various nutrients, including protein, polysaccharides, crude fiber, amino acids, and trace elements such as iron, magnesium, potassium, sodium, zinc, calcium, and phosphorus. This makes its resource utilization highly valuable. Currently, the main avenues for the resource utilization of Enteromorpha include food, medicine, feed, and low-value fertilizer. However, these resource-based products have low consumption, long production cycles, and high costs, making them unable to meet the rapidly growing and rampant demand for Enteromorpha treatment.

[0004] Existing enteromorpha humification technology mainly uses microbial agents. For example, the patent with authorization announcement number CN103772073B discloses a enteromorpha bio-organic fertilizer and its preparation method and application. The enteromorpha bio-organic fertilizer includes the following components: enteromorpha residue, cassava residue, bentonite, phosphogypsum, calcium powder and EM composite bacterial powder. The EM composite bacterial powder used can also regulate the soil microecological environment. It can utilize the nutrients in the enteromorpha residue to reproduce on the soil surface, forming a layer of microbial protection barrier to prevent other pests and diseases from infecting crops, improve the crop's stress resistance, and play a role in resisting continuous cropping and preventing diseases.

[0005] However, the enteromorpha humification technology using microbial agents has problems such as long composting cycle, incomplete composting, large area occupied, and low fertilizer activity.

[0006] Therefore, the development of rapid humification technology for Enteromorpha and methods for preparing highly active fertilizers are of great significance for solving the problems of marine pollution and resource waste caused by Enteromorpha. It also provides a new technical path for the resource utilization and high-value utilization of Enteromorpha. Summary of the Invention

[0007] The purpose of the present invention is to solve the problems existing in the prior art and provide a method for rapid humification of enteromorpha and its application.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] A method for rapidly humifying enteromorpha comprises humifying enteromorpha by a hydrothermal method under the action of a catalyst; the catalyst is one or more of nano-ferric oxide, nano-ferric chloride, nano-cuprous chloride and ammonium sulfite.

[0010] The present invention utilizes a hydrothermal method to humify Enteromorpha under the action of a catalyst to obtain a substance rich in fulvic acid. This is because the hydrothermal method can effectively extract amino acids and polysaccharides from Enteromorpha. Under high temperature and high pressure, nano-ferric oxide, nano-ferric chloride, and nano-cuprous chloride catalyze the decomposition of water in Enteromorpha, generating hydroxyl radicals and superoxide radicals. Ammonium sulfite generates sulfate radicals. These radicals attack the extracted amino acids and polysaccharides, causing them to undergo a Maillard reaction and convert them into fulvic / humic acid substances. Furthermore, the catalyst improves the efficiency of the hydrothermal reaction, shortening the reaction time to 1-3 hours, which is 1 / 6-1 / 3 of the time of traditional hydrothermal methods.

[0011] As the preferred technical solution:

[0012] In the method for rapid humification of Enteromorpha as described above, the amount of catalyst added is 5-15 wt % of the dry weight of Enteromorpha.

[0013] The method for rapid humification of Enteromorpha as described above comprises the following steps:

[0014] (a) washing fresh Enteromorpha (pH value after washing is 5-7), crushing (mesh size after crushing is 2-10 mesh), maintaining the moisture content at 60-90 wt%, and then adding the crushed Enteromorpha to an autoclave;

[0015] (b) After adding the catalyst into the autoclave, the reaction was carried out at 140-180° C. and 1-3 MPa for 1-3 hours.

[0016] The method for rapid humification of enteromorpha as described above further comprises the following steps:

[0017] (c) grinding the product of step (b) (to a mesh size of 1-5 mesh after grinding), and then compounding it with biochar to obtain a humified solid product;

[0018] (d) compounding the product of step (c) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer.

[0019] In the method for rapid humification of Enteromorpha as described above, in step (c), the biochar is straw-based biochar; and the amount of biochar added is 10-20% of the dry weight of Enteromorpha.

[0020] According to the method for rapid humification of Enteromorpha as described above, in step (d), the biological agent is one or more of Bacillus, yeast, nitrogen-fixing bacteria and photosynthetic bacteria, and the effective viable count (CFU) is ≥ 20 million / g; and the mass ratio of the product of step (c) to the biological agent is 85-95:5-15.

[0021] The present invention also provides a Enteromorpha-based bio-organic fertilizer, which is prepared by the Enteromorpha rapid humification method described above, and has a fulvic acid content of ≥20wt% and a humic acid content of 15-18wt%.

[0022] Beneficial effects:

[0023] (1) The present invention utilizes a hydrothermal method in combination with a specific catalyst to achieve rapid humification of Enteromorpha, significantly shortening the humification process time and improving the treatment efficiency.

[0024] (2) Through the method of the present invention, amino acids and polysaccharides in Enteromorpha undergo Maillard reaction under the action of high temperature, high pressure and catalyst, and are converted into highly active fertilizers rich in fulvic acid substances. Fulvic acid substances have excellent soil improvement and plant growth promotion effects, thereby improving the value and application effect of fertilizers.

[0025] (3) The present invention provides an effective way to utilize Enteromorpha as a resource, converting Enteromorpha into a highly active fertilizer, which helps to solve the problem of marine pollution caused by Enteromorpha outbreaks, reduces the threat of Enteromorpha to the marine ecological environment, and realizes the resource utilization of waste, reducing resource waste.

[0026] (4) Compared with the traditional microbial agent humification technology, the present invention has the advantages of short composting cycle, thorough composting, and small footprint, which reduces the cost and difficulty of Ulva treatment. At the same time, due to the short reaction time, it also reduces energy consumption and manpower input. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The figures are pictures and three-dimensional fluorescence spectra of Enteromorpha before and after humification in Example 1 of the present invention; in the figure, a is a picture of fresh Enteromorpha, b is a three-dimensional fluorescence spectra of Figure a, c is a picture of the product after Enteromorpha humification, and d is a three-dimensional fluorescence spectra of Figure c, wherein I and II are mixtures of amino acids and protein small molecules, III is fulvic acid, IV is a soluble microbial metabolite, and V is humic acid;

[0028] Figure 2 1 is a UV-visible absorption spectrum of fresh Enteromorpha and Enteromorpha humification product in Example 1 of the present invention;

[0029] Figure 3This is a fertilizer effect diagram of wheat hydroponically cultivated with different hydroponic nutrient solutions for 12 days according to the present invention; in the figure, the first row from top to bottom is a top view of wheat hydroponically cultivated with different hydroponic nutrient solutions for 12 days, the second row is a front view of wheat hydroponically cultivated with different hydroponic nutrient solutions for 12 days, and each row from left to right is a fertilizer effect diagram of control group 1 (hydroponic nutrient solution is deionized water), control group 2 (using the humified product of Enteromorpha as fertilizer), control group 3 (using fresh Enteromorpha as fertilizer), control group 4 (using seaweed liquid as fertilizer), and a diagram of the fertilizer effect of wheat hydroponically incubated with the Enteromorpha-based bio-organic fertilizer in Example 1 as fertilizer;

[0030] Figure 4 Schematic diagram of the growth promotion results of hydroponic incubation of wheat using Enteromorpha-based bio-organic fertilizer in Control Group 1, Control Group 2, Control Group 3, Control Group 4 and Example 1 of the present invention;

[0031] Figure 5 Schematic diagram of the growth-promoting effects of deionized water, Enteromorpha, and Enteromorpha bio-organic fertilizer extracts of different mass concentrations on cucumber germination;

[0032] Figure 6 3D fluorescence analysis graphs of the Enteromorpha-based bio-organic fertilizer prepared in Example 1 of the present invention and the fertilizer prepared in Comparative Example 1; in the figure, a is a 3D fluorescence analysis graph of fresh Enteromorpha in Comparative Example 1, and b is a 3D fluorescence analysis graph of the Enteromorpha bio-organic fertilizer prepared in Example 1, wherein I and II are mixtures of amino acids and protein small molecules, III is fulvic acid, IV is a soluble microbial metabolite, and V is humic acid. DETAILED DESCRIPTION

[0033] Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiments are only used to illustrate the present invention and are not used in limiting the scope of the present invention.In addition, should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall equally within the scope limited by the appended claims of the application.

[0034] The following are the test methods for the relevant performance indicators in each embodiment and comparative example:

[0035] Fulvic acid content: The Enteromorpha-based bio-organic fertilizers prepared in each example were used as samples, and the fulvic acid content of the samples was determined according to the NY / T 3162-2017 "Determination of Fulvic Acid in Fertilizers - Volumetric Titration Method".

[0036] Humic acid content: The Enteromorpha-based bio-organic fertilizers prepared in each example were used as samples, and the humic acid content in the samples was determined according to NY / T1971-2010 "Determination of Humic Acid Content in Water-Soluble Fertilizers".

[0037] Germination index: The Enteromorpha-based bio-organic fertilizer prepared in each embodiment was used as a sample, and then the seed germination index of the sample was measured with reference to the standard of NY T525-2021 "Organic Fertilizer"; wherein, the seeds used in the test were cucumber seeds.

[0038] In the following embodiments, all operations without explicit temperature conditions are assumed to be performed at room temperature, which is in the range of 22-25°C.

[0039] Example 1

[0040] A method for preparing enteromorpha-based bio-organic fertilizer, comprising the following steps:

[0041] (1) Preparation of materials;

[0042] Fresh Enteromorpha;

[0043] Catalyst: nano-ferric oxide;

[0044] Biochar: Straw-based biochar, manufactured by Zhengzhou Xingsen Activated Carbon Co., Ltd., with a mesh size of 2-10 mesh;

[0045] Biological agent: Bacillus, manufactured by Guangxi Nongbao Bioengineering Co., Ltd., with an effective viable count of 25 million / g;

[0046] (2) washing fresh Enteromorpha (pH value after washing is 5), crushing (mesh size after crushing is 2 mesh), and adding it into the autoclave after maintaining the moisture content at 60 wt%;

[0047] (3) After adding the catalyst to the autoclave, the reaction was carried out at 160°C and 1 MPa for 3 h to obtain the humified product of Enteromorpha (the UV-visible absorption spectrum and three-dimensional fluorescence spectrum of the product were compared with those of fresh Enteromorpha. The results are shown in the figure). Figure 1 、 Figure 2 As shown in the figure, it can be seen that compared with fresh Enteromorpha, the content of fulvic acid and humic acid substances in the product after the humification reaction increases, indicating that Enteromorpha contains rich fulvic acid and humic acid substances after the humification reaction); wherein, the amount of catalyst added is 15wt% of the dry weight of Enteromorpha;

[0048] (4) grinding the product of step (3) (grinding to a mesh size of 1 mesh), and then compounding it with biochar to obtain a humified solid product; wherein the amount of biochar added is 10 wt% of the dry weight of Enteromorpha;

[0049] (5) compounding the product of step (4) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer; wherein the mass ratio of the product of step (4) to the biological agent is 85:15.

[0050] The content of fulvic acid in the finally prepared Enteromorpha-based bio-organic fertilizer is 22 wt % and the content of humic acid is 15 wt %;

[0051] like Figure 5 As shown, refer to NYT 525-2021 "Organic Fertilizer" standard measured that the cucumber seed germination index when deionized water is used as the hydroponic nutrient solution is 50%; when using hydroponic nutrient solution A (fresh Enteromorpha is dissolved in deionized water to prepare a hydroponic nutrient solution with a concentration of 50 mg / L), the cucumber seed germination index is 40%; when using hydroponic nutrient solution B (the humification product of Enteromorpha is dissolved in deionized water to prepare a hydroponic nutrient solution with a concentration of 50 mg / L), the cucumber seed germination index is 75%; when using hydroponic nutrient solution C (seaweed liquid is dissolved in deionized water to prepare a hydroponic nutrient solution with a concentration of 50 mg / L), the cucumber seed germination index is 72%; when using hydroponic nutrient solution D (the above-mentioned Enteromorpha biological organic fertilizer is dissolved in deionized water to prepare a hydroponic nutrient solution with a concentration of 50 mg / L), the cucumber seed germination index is 90%; through the above comparison, it can be seen that Enteromorpha-based biological organic fertilizer is thoroughly decomposed, has higher fertilizer efficiency, and is more conducive to cultivating seed germination, thereby promoting crop growth.

[0052] The above-mentioned Enteromorpha-based bio-organic fertilizer is used to hydroponically incubate wheat, and the specific steps are as follows:

[0053] (a) preparing a hydroponic nutrient solution;

[0054] The Enteromorpha-based bio-organic fertilizer prepared above was dissolved in deionized water, mixed evenly, and a hydroponic nutrient solution with a concentration of 50 mg / L was prepared;

[0055] (b) treating wheat seeds;

[0056] Wheat seeds (manufacturer: Jingyan Yinong (Beijing) Seed Technology Co., Ltd.) were first soaked in tap water for 24 h in the dark, then sterilized with 70% ethanol solution, and finally rinsed with deionized water for later use.

[0057] (c) hydroponic incubation;

[0058] An 11 cm culture dish is used as a container for hydroponic wheat cultivation, and then two filter papers are placed at the bottom of the culture dish, respectively. Wheat seeds treated in step (b) are placed on the filter papers at intervals, and then 15 mL of the hydroponic nutrient solution prepared in step (a) is added thereto. Finally, the culture dish is placed in an artificial incubator and cultured for 12 days at a temperature of 25° C. and a humidity of 50%. The number of wheat seeds in the culture dish is 9, and 20 mL of deionized water is added to the culture dish every other day during the culture process. The light period in the artificial incubator is 9:00-21:00, and the dark period is 21:00-9:00.

[0059] After 12 days of hydroponic culture, the fresh weight, plant height, root length and chlorophyll content of each wheat in the culture dish were measured, and the average values ​​were calculated. It was found that after 12 days of hydroponic culture, the average fresh weight of the wheat was 2.2g, the average plant height was 10.09cm, the average root length was 8.98cm, and the average chlorophyll content (SPAD) was 34;

[0060] Then 4 control groups were compared with the above hydroponic results. The 4 control groups are as follows:

[0061] Control group 1: The steps for hydroponic incubation of wheat were basically the same as above, except that the Enteromorpha-based bio-organic fertilizer was replaced with deionized water of equal mass;

[0062] After 12 days of hydroponic cultivation, the average fresh weight of wheat was 1.3 g, the average plant height was 6 cm, the average root length was 4.9 cm, and the average chlorophyll content (SPAD) was 11;

[0063] Control group 2: The steps of hydroponic incubation of wheat were basically the same as the above process, except that the Enteromorpha-based bio-organic fertilizer was replaced with an equal mass of the Enteromorpha humified product prepared in Example 1;

[0064] After 12 days of hydroponic cultivation, the average fresh weight of wheat was 1.5 g, the average plant height was 9 cm, the average root length was 8.2 cm, and the average chlorophyll content (SPAD) was 16;

[0065] Control group 3: The steps for hydroponic incubation of wheat were basically the same as the above process, except that the Enteromorpha-based bio-organic fertilizer was replaced with Enteromorpha of equal mass;

[0066] After 12 days of hydroponic cultivation, the average fresh weight of wheat was 0.8 g, the average plant height was 5.2 cm, the average root length was 4 cm, and the average chlorophyll content (SPAD) was 8;

[0067] Control group 4: The steps for hydroponic incubation of wheat were basically the same as the above process, except that the Enteromorpha-based bio-organic fertilizer was replaced with an equal mass of alginic acid; wherein the alginic acid was obtained by extracting Enteromorpha using a 0.1 mol / L Na2CO3 aqueous solution;

[0068] After 12 days of hydroponic cultivation, the average fresh weight of wheat was 1.4 g, the average plant height was 5.8 cm, the average root length was 5.3 cm, and the average chlorophyll content (SPAD) was 20;

[0069] The results of the above hydroponic culture are as follows Figure 3 、 Figure 4As shown in the figure, it can be seen that the Enteromorpha-based bio-organic fertilizer prepared by the present invention is more conducive to wheat germination and promotes the development of wheat root system, thereby making the fertilizer effect significantly better.

[0070] Comparative Example 1

[0071] A method for preparing a fertilizer, which differs from Example 1 only in that no catalyst is added in step (3).

[0072] The content of fulvic acid in the final fertilizer is 3 wt %, and the content of humic acid is 1.5 wt %.

[0073] Comparing Comparative Example 1 with Example 1, it can be seen that ( Figure 6 The content of fulvic acid in the fertilizer prepared in Comparative Example 1 is low. This is because, in the absence of a catalyst, the water in the enteromorpha cannot be catalytically decomposed to produce hydroxyl radicals and superoxide radicals, making it difficult for the Maillard reaction to occur to convert the fulvic acid / humic acid substances. In addition, the efficiency of the hydrothermal reaction is reduced, and fulvic acid and humic acid cannot be efficiently generated in a short time, ultimately resulting in low contents of fulvic acid and humic acid.

[0074] Example 2

[0075] A method for preparing enteromorpha-based bio-organic fertilizer, comprising the following steps:

[0076] (1) Preparation of materials;

[0077] Fresh Enteromorpha;

[0078] Catalyst: nano iron oxide;

[0079] Biochar: Straw-based biochar, manufactured by Zhengzhou Xingsen Activated Carbon Co., Ltd., with a mesh size of 2-10 mesh;

[0080] Biological agent: yeast, manufactured by Guangxi Nongbao Bioengineering Co., Ltd., with an effective viable count of 30 million / g;

[0081] (2) washing fresh Enteromorpha (pH value after washing is 5), crushing (mesh size after crushing is 4 mesh), and adding it into the autoclave after maintaining the moisture content at 75 wt%;

[0082] (3) adding a catalyst to a high-pressure reactor and reacting at 165° C. and 2 MPa for 1.5 h to obtain a humified product of Enteromorpha; wherein the amount of the catalyst added is 6 wt% of the dry weight of Enteromorpha;

[0083] (4) grinding the product of step (3) (grinding to a mesh size of 2 mesh), and then compounding it with biochar to obtain a humified solid product; wherein the amount of biochar added is 20 wt% of the dry weight of Enteromorpha;

[0084] (5) compounding the product of step (4) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer; wherein the mass ratio of the product of step (4) to the biological agent is 85:15.

[0085] The content of fulvic acid in the finally prepared Enteromorpha-based bio-organic fertilizer is 23 wt %, and the content of humic acid is 15 wt %.

[0086] The above-mentioned Enteromorpha-based bio-organic fertilizer is used to hydroponically incubate wheat, and the specific steps are as follows:

[0087] (a) preparing a hydroponic nutrient solution;

[0088] The Enteromorpha-based bio-organic fertilizer prepared above was dissolved in deionized water, mixed evenly, and a hydroponic nutrient solution with a concentration of 50 mg / L was prepared;

[0089] (b) treating wheat seeds;

[0090] Wheat seeds (manufacturer: Jingyan Yinong (Beijing) Seed Technology Co., Ltd.) were first soaked in tap water for 24 h in the dark, then sterilized with 70% ethanol, and finally rinsed with deionized water for later use.

[0091] (c) hydroponic incubation;

[0092] An 11 cm culture dish is used as a container for hydroponic wheat cultivation, and then two filter papers are placed at the bottom of the culture dish, respectively. Wheat seeds treated in step (b) are placed on the filter papers at intervals, and then 15 mL of the hydroponic nutrient solution prepared in step (a) is added thereto. Finally, the culture dish is placed in an artificial incubator and cultured for 12 days at a temperature of 25° C. and a humidity of 50%. The number of wheat seeds in the culture dish is 9, and 20 mL of deionized water is added to the culture dish every other day during the culture process. The light period in the artificial incubator is 9:00-21:00, and the dark period is 21:00-9:00.

[0093] After 12 days of hydroponics, the fresh weight, plant height, root length and chlorophyll of each wheat in the culture dish were measured, and the average values ​​were calculated. It was found that after 12 days of hydroponics, the average fresh weight of the wheat was 2.2g, the average plant height was 10.12cm, the average root length was 8.95cm, and the average chlorophyll content (SPAD) was 32.25.

[0094] Comparative Example 2

[0095] A method for preparing a fertilizer, which differs from Example 2 only in that no catalyst is added in step (3).

[0096] The content of fulvic acid in the final fertilizer is 5 wt%.

[0097] Comparing Example 1 with Comparative Example 2, it can be seen that the content of fulvic acid in the fertilizer of Comparative Example 2 is significantly reduced. This is because in the absence of a catalyst, the water in the Enteromorpha cannot be effectively catalyzed and decomposed to produce hydroxyl radicals and superoxide radicals, resulting in the lack of sufficient free radical attack on the extracted amino acids and polysaccharides, making it difficult for the Maillard reaction to occur and convert them into fulvic acid-like substances. At the same time, the efficiency of the hydrothermal reaction is reduced, and fulvic acid cannot be efficiently generated in a short time.

[0098] Example 3

[0099] A method for preparing enteromorpha-based bio-organic fertilizer, comprising the following steps:

[0100] (1) Preparation of materials;

[0101] Fresh Enteromorpha;

[0102] Catalyst: nano cuprous chloride;

[0103] Biochar: Straw-based biochar, manufactured by Zhengzhou Xingsen Activated Carbon Co., Ltd., with a mesh size of 2-10 mesh;

[0104] Biological agent: nitrogen-fixing bacteria, manufactured by Guangxi Nongbao Bioengineering Co., Ltd., with an effective viable bacterial count of 25 million / g;

[0105] (2) washing fresh Enteromorpha (pH value after washing is 6), crushing (mesh size after crushing is 8 mesh), and adding it into the autoclave after maintaining the moisture content at 85 wt%;

[0106] (3) adding a catalyst to a high-pressure reactor and reacting at 180° C. and 3 MPa for 2 h to obtain a humified product of Enteromorpha; wherein the amount of the catalyst added is 8 wt% of the dry weight of Enteromorpha;

[0107] (4) grinding the product of step (3) (grinding to a mesh size of 3 mesh), and then compounding it with biochar to obtain a humified solid product; wherein the amount of biochar added is 15 wt% of the dry weight of Enteromorpha;

[0108] (5) compounding the product of step (4) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer; wherein the mass ratio of the product of step (4) to the biological agent is 90:10.

[0109] The content of fulvic acid in the finally prepared Enteromorpha-based bio-organic fertilizer is 20 wt %, and the content of humic acid is 18 wt %.

[0110] The above-mentioned Enteromorpha-based bio-organic fertilizer is used to hydroponically incubate wheat, and the specific steps are as follows:

[0111] (a) preparing a hydroponic nutrient solution;

[0112] The Enteromorpha-based bio-organic fertilizer prepared above was dissolved in deionized water, mixed evenly, and a hydroponic nutrient solution with a concentration of 50 mg / L was prepared;

[0113] (b) treating wheat seeds;

[0114] Wheat seeds (manufacturer: Jingyan Yinong (Beijing) Seed Technology Co., Ltd.) were first soaked in tap water for 24 h in the dark, then sterilized with 70% ethanol, and finally rinsed with deionized water for later use.

[0115] (c) hydroponic incubation;

[0116] An 11 cm culture dish is used as a container for hydroponic wheat cultivation, and then two filter papers are placed at the bottom of the culture dish, respectively. Wheat seeds treated in step (b) are placed on the filter papers at intervals, and then 15 mL of the hydroponic nutrient solution prepared in step (a) is added thereto. Finally, the culture dish is placed in an artificial incubator and cultured for 12 days at a temperature of 25° C. and a humidity of 50%. The number of wheat seeds in the culture dish is 9, and 20 mL of deionized water is added to the culture dish every other day during the culture process. The light period in the artificial incubator is 9:00-21:00, and the dark period is 21:00-9:00.

[0117] After 12 days of hydroponics, the fresh weight, plant height, root length and chlorophyll of each wheat in the culture dish were measured, and the average values ​​were calculated. It was found that after 12 days of hydroponics, the average fresh weight of the wheat was 2.1 g, the average plant height was 9.98 cm, the average root length was 9.12 cm, and the average chlorophyll content (SPAD) was 32.48.

[0118] Example 4

[0119] A method for preparing enteromorpha-based bio-organic fertilizer, comprising the following steps:

[0120] (1) Preparation of materials;

[0121] Fresh Enteromorpha;

[0122] Catalyst: ammonium sulfite;

[0123] Biochar: Straw-based biochar, manufactured by Zhengzhou Xingsen Activated Carbon Co., Ltd., with a mesh size of 2-10 mesh;

[0124] Biological agent: photosynthetic bacteria, manufactured by Guangxi Nongbao Bioengineering Co., Ltd., with an effective viable bacterial count of 30 million / g;

[0125] (2) washing fresh Enteromorpha (pH value after washing is 6), crushing (mesh size after crushing is 5 mesh), and adding it into the autoclave after maintaining the moisture content at 80 wt%;

[0126] (3) adding a catalyst to a high-pressure reactor and reacting at 140° C. and 2 MPa for 2.5 h to obtain a humified product of Enteromorpha; wherein the amount of the catalyst added is 10 wt % of the dry weight of Enteromorpha;

[0127] (4) grinding the product of step (3) (grinding to a mesh size of 4 mesh), and then compounding it with biochar to obtain a humified solid product; wherein the amount of biochar added is 12 wt% of the dry weight of Enteromorpha;

[0128] (5) compounding the product of step (4) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer; wherein the mass ratio of the product of step (4) to the biological agent is 88:12.

[0129] The content of fulvic acid in the finally prepared Enteromorpha-based bio-organic fertilizer is 25 wt %, and the content of humic acid is 16 wt %.

[0130] The above-mentioned Enteromorpha-based bio-organic fertilizer is used to hydroponically incubate wheat, and the specific steps are as follows:

[0131] (a) preparing a hydroponic nutrient solution;

[0132] The Enteromorpha-based bio-organic fertilizer prepared above was dissolved in deionized water, mixed evenly, and a hydroponic nutrient solution with a concentration of 50 mg / L was prepared;

[0133] (b) treating wheat seeds;

[0134] Wheat seeds (manufacturer: Jingyan Yinong (Beijing) Seed Technology Co., Ltd.) were first soaked in tap water for 24 h in the dark, then sterilized with 70% ethanol, and finally rinsed with deionized water for later use.

[0135] (c) hydroponic incubation;

[0136] An 11 cm culture dish is used as a container for hydroponic wheat cultivation, and then two filter papers are placed at the bottom of the culture dish, respectively. Wheat seeds treated in step (b) are placed on the filter papers at intervals, and then 15 mL of the hydroponic nutrient solution prepared in step (a) is added thereto. Finally, the culture dish is placed in an artificial incubator and cultured for 12 days at a temperature of 25° C. and a humidity of 50%. The number of wheat seeds in the culture dish is 9, and 20 mL of deionized water is added to the culture dish every other day during the culture process. The light period in the artificial incubator is 9:00-21:00, and the dark period is 21:00-9:00.

[0137] After 12 days of hydroponics, the fresh weight, plant height, root length and chlorophyll of each wheat in the culture dish were measured, and the average values ​​were calculated. It was found that after 12 days of hydroponics, the average fresh weight of the wheat was 2.05 g, the average plant height was 10.23 cm, the average root length was 9.23 cm, and the average chlorophyll content (SPAD) was 33.14.

[0138] Example 5

[0139] A method for preparing enteromorpha-based bio-organic fertilizer, comprising the following steps:

[0140] (1) Preparation of materials;

[0141] Fresh Enteromorpha;

[0142] Catalyst: a mixture of nano-iron oxide, cuprous chloride, and ammonium sulfate in a mass ratio of 1:1:3;

[0143] Biochar: Straw-based biochar, manufactured by Zhengzhou Xingsen Activated Carbon Co., Ltd., with a mesh size of 2-10 mesh;

[0144] Biological agent: a mixture of Bacillus and yeast in a mass ratio of 70:30, manufactured by Guangxi Nongbao Bioengineering Co., Ltd., with an effective viable count of 20 million / g;

[0145] (2) washing fresh Enteromorpha (pH value after washing is 7), crushing (mesh size after crushing is 10 mesh), and adding it into the autoclave after maintaining the moisture content at 90 wt%;

[0146] (3) adding a catalyst to a high-pressure reactor and reacting at 150° C. and 1 MPa for 1 hour to obtain a humified product of Enteromorpha; wherein the amount of the catalyst added is 5 wt% of the dry weight of Enteromorpha;

[0147] (4) grinding the product of step (3) (grinding to a mesh size of 5 mesh), and then compounding it with biochar to obtain a humified solid product; wherein the amount of biochar added is 12 wt% of the dry weight of Enteromorpha;

[0148] (5) compounding the product of step (4) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer; wherein the mass ratio of the product of step (4) to the biological agent is 90:10.

[0149] The content of fulvic acid in the finally prepared Enteromorpha-based bio-organic fertilizer is 25 wt %, and the content of humic acid is 17 wt %.

[0150] The above-mentioned Enteromorpha-based bio-organic fertilizer is used to hydroponically incubate wheat, and the specific steps are as follows:

[0151] (a) preparing a hydroponic nutrient solution;

[0152] The Enteromorpha-based bio-organic fertilizer prepared above was dissolved in deionized water, mixed evenly, and a hydroponic nutrient solution with a concentration of 50 mg / L was prepared;

[0153] (b) treating wheat seeds;

[0154] Wheat seeds (manufacturer: Jingyan Yinong (Beijing) Seed Technology Co., Ltd.) were first soaked in tap water for 24 h in the dark, then sterilized with 70% ethanol, and finally rinsed with deionized water for later use.

[0155] (c) hydroponic incubation;

[0156] An 11 cm culture dish is used as a container for hydroponic wheat cultivation, and then two filter papers are placed at the bottom of the culture dish, respectively. Wheat seeds treated in step (b) are placed on the filter papers at intervals, and then 15 mL of the hydroponic nutrient solution prepared in step (a) is added thereto. Finally, the culture dish is placed in an artificial incubator and cultured for 12 days at a temperature of 25° C. and a humidity of 50%. The number of wheat seeds in the culture dish is 9, and 20 mL of deionized water is added to the culture dish every other day during the culture process. The light period in the artificial incubator is 9:00-21:00, and the dark period is 21:00-9:00.

[0157] After 12 days of hydroponics, the fresh weight, plant height, root length and chlorophyll of each wheat in the culture dish were measured, and the average values ​​were calculated. It was found that after 12 days of hydroponics, the average fresh weight of the wheat was 2.15 g, the average plant height was 10.07 cm, the average root length was 9.02 cm, and the average chlorophyll content (SPAD) was 32.76.

[0158] Example 6

[0159] A method for preparing enteromorpha-based bio-organic fertilizer, comprising the following steps:

[0160] (1) Preparation of materials;

[0161] Fresh Enteromorpha;

[0162] Catalyst: nano-ferric oxide;

[0163] Biochar: Straw-based biochar, manufactured by Zhengzhou Xingsen Activated Carbon Co., Ltd., with a mesh size of 2-10 mesh;

[0164] Biological agent: yeast, manufactured by Guangxi Nongbao Bioengineering Co., Ltd., with an effective viable count of 30 million / g;

[0165] (2) washing fresh Enteromorpha (pH value after washing is 6), crushing (mesh size after crushing is 4 mesh), and adding it to the autoclave after maintaining the moisture content at 80 wt%;

[0166] (3) adding a catalyst to a high-pressure reactor and reacting at 140° C. and 3 MPa for 2 h to obtain a humified product of Enteromorpha; wherein the amount of the catalyst added is 15 wt% of the dry weight of Enteromorpha;

[0167] (4) grinding the product of step (3) (grinding to a mesh size of 3 mesh), and then compounding it with biochar to obtain a humified solid product; wherein the amount of biochar added is 15% of the dry weight of Enteromorpha;

[0168] (5) compounding the product of step (4) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer; wherein the mass ratio of the product of step (4) to the biological agent is 95:5.

[0169] The content of fulvic acid in the finally prepared Enteromorpha-based bio-organic fertilizer is 21 wt %, and the content of humic acid is 18 wt %.

[0170] The above-mentioned Enteromorpha-based bio-organic fertilizer is used to hydroponically incubate wheat, and the specific steps are as follows:

[0171] (a) preparing a hydroponic nutrient solution;

[0172] The Enteromorpha-based bio-organic fertilizer prepared above was dissolved in deionized water, mixed evenly, and a hydroponic nutrient solution with a concentration of 50 mg / L was prepared;

[0173] (b) treating wheat seeds;

[0174] Wheat seeds (manufacturer: Jingyan Yinong (Beijing) Seed Technology Co., Ltd.) were first soaked in tap water for 24 h in the dark, then sterilized with 70% ethanol, and finally rinsed with deionized water for later use.

[0175] (c) hydroponic incubation;

[0176] An 11 cm culture dish is used as a container for hydroponic wheat cultivation, and then two filter papers are placed at the bottom of the culture dish, respectively. Wheat seeds treated in step (b) are placed on the filter papers at intervals, and then 15 mL of the hydroponic nutrient solution prepared in step (a) is added thereto. Finally, the culture dish is placed in an artificial incubator and cultured for 12 days at a temperature of 25° C. and a humidity of 50%. The number of wheat seeds in the culture dish is 9, and 20 mL of deionized water is added to the culture dish every other day during the culture process. The light period in the artificial incubator is 9:00-21:00, and the dark period is 21:00-9:00.

[0177] After 12 days of hydroponics, the fresh weight, plant height, root length and chlorophyll of each wheat in the culture dish were measured, and the average values ​​were calculated. It was found that after 12 days of hydroponics, the average fresh weight of the wheat was 2.13 g, the average plant height was 9.98 cm, the average root length was 9.13 cm, and the average chlorophyll content (SPAD) was 31.98.

Claims

1. A method for rapid humification of Enteromorpha, characterized in that: Under the action of a catalyst, the enteromorpha is humified by a hydrothermal method; the catalyst is one or more of nano-ferric oxide, nano-ferric chloride, nano-cuprous chloride and ammonium sulfite.

2. The method for rapid humification of Enteromorpha according to claim 1, wherein: The added amount of the catalyst is 5-15 wt% of the dry weight of Enteromorpha.

3. The method for rapid humification of Enteromorpha according to claim 1 or 2, characterized in that: The following steps are involved: (a) washing and crushing fresh Enteromorpha in sequence, maintaining the moisture content at 60-90 wt %, and then adding the crushed Enteromorpha to an autoclave; (b) After adding the catalyst into the autoclave, the reaction was carried out at 140-180° C. and 1-3 MPa for 1-3 hours.

4. The method for rapid humification of enteromorpha according to claim 3, characterized in that: The following steps are also included: (c) grinding the product of step (b) and then compounding it with biochar; (d) compounding the product of step (c) with a biological agent to obtain an enteromorpha-based bio-organic fertilizer.

5. The method for rapid humification of enteromorpha according to claim 4, characterized in that: In step (c), the biochar is straw-based biochar; and the amount of biochar added is 10-20% of the dry weight of Enteromorpha.

6. The method for rapid humification of enteromorpha according to claim 4, characterized in that: In step (d), the biological agent is one or more of Bacillus, yeast, nitrogen-fixing bacteria and photosynthetic bacteria, and the effective viable count is ≥ 20 million / g; the mass ratio of the product of step (c) to the biological agent is 85-95:5-15.

7. A Enteromorpha-based bio-organic fertilizer, characterized in that: The humic acid is prepared by the method for rapid humification of enteromorpha as claimed in any one of claims 4 to 6, and the content of fulvic acid is ≥20wt%.

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