Active protease composition for expelling toxins and purifying skin
By combining a cross-source protease system with functional adjuvants, the problems of limited action range and insufficient stability of proteases in existing skin care products are solved, all-round purification and protection of the skin is achieved, and the antioxidant and anti-inflammatory functions are enhanced.
Patent Information
- Application Number
- CN202510619750.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-14
- Publication Date
- 2025-09-26
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In existing skin care products, the action range of a single protease is limited and it cannot completely break down impurities and toxins on the skin surface. In addition, the activity of the protease is easily affected by the environment and it lacks antioxidant and anti-inflammatory functions, leading to oxidative damage and inflammatory reactions to the skin.
A cross-source protease system is constructed, combining plant-derived and microbial-derived proteases, and using grape seed extract and green tea extract rich in proanthocyanidins as functional adjuvants to regulate pH, scavenge free radicals, enhance the stability of protease activity, and form a moisturizing film through glycerin and xanthan gum to provide antioxidant and anti-inflammatory protection.
It achieves extensive decomposition of skin impurities and toxins, improves the stability of proteases, repairs skin inflammatory damage, resists oxidative damage, and provides comprehensive skin care effects.
Smart Images
Figure CN120694923A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of protease compositions, in particular to an active protease composition for detoxification and skin purification. Background Art
[0002] As the largest organ of the human body, the skin is in direct contact with the external environment and is prone to accumulate various impurities and toxins, such as dust, pollutants, free radicals, and aging cuticles produced by its own metabolism. If these substances accumulate for a long time, they will affect the normal metabolism and respiration of the skin, causing dullness, roughness, acne, inflammation and other problems on the skin, which not only affects the appearance, but may also damage the health of the skin. Therefore, detoxification, purification, and keeping the skin clean and healthy are crucial to maintaining good skin appearance and physiological function.
[0003] Active proteases play a key role in the detoxification and purification of the skin. Their mechanism of action is mainly through the catalytic activity of enzymes to specifically identify and decompose impurities and toxins on the skin surface and in the pores. For example, some proteases can hydrolyze protein impurities and break them down into small molecules for easy excretion by the skin; some proteases can promote the shedding of aging stratum corneum and accelerate the renewal of skin cells, thereby achieving detoxification and purification of the skin.
[0004] However, the existing technology has many problems when using active proteases for skin care. On the one hand, most products only use a single protease, which has a limited range of action and cannot completely decompose various impurities and toxins on the skin surface. On the other hand, the existing technology lacks effective protection for the stability of protease activity. During storage and use, the activity of protease is easily affected by environmental factors and reduced, resulting in poor product effect. In addition, existing products often only focus on detoxification and purification functions, ignoring the oxidative damage and inflammatory reactions that the skin may suffer during the detoxification process. Therefore, it is of great significance to develop an active protease composition that detoxifies and purifies the skin. Summary of the Invention
[0005] The purpose of the present invention is to make up for the shortcomings of the existing technology and provide an active protease composition for detoxification and skin purification. It can accurately match proteases from different sources by constructing a cross-source protease-functional adjuvant synergistic network, expand the range of decomposition substrates, and more comprehensively remove skin impurities and toxins. It uses functional adjuvants to adjust the pH value of the system, eliminate free radicals, and improve the stability of protease activity to ensure that the product can effectively function in different environments. At the same time, with the help of the antioxidant, anti-inflammatory and chelating functions of functional adjuvants, it can simultaneously repair skin inflammatory damage and resist environmental oxidative damage during the detoxification and purification process, providing comprehensive care for the skin.
[0006] To solve the above technical problems, the present invention provides the following technical solution: an active protease composition for detoxification and skin purification, the composition comprising the following components: A cross-source protease system, consisting of a plant-derived protease and a microbial-derived protease, wherein the mass ratio of the plant-derived protease to the microbial-derived protease is 1:0.5-1.5; A functional adjuvant system comprising a grape seed extract rich in proanthocyanidins and a green tea extract with heavy metal chelating ability, wherein the mass ratio of the grape seed extract to the green tea extract is 1:0.8-1.2, and the mass ratio of the functional adjuvant system in the composition is 5%-15%; A humectant, a thickener and a solvent, wherein the humectant is glycerin, which accounts for 5% to 10% by weight of the composition; the thickener is xanthan gum, which accounts for 0.5% to 1.5% by weight of the composition; and the solvent is deionized water of high purity and free of impurity ions, with the remainder added.
[0007] Furthermore, the plant-derived protease is papain, whose molecular structure recognizes and binds to specific protein structures in aged keratin of the skin, interacts with the peptide bonds of aged keratin proteins through active sites, catalyzes the hydrolysis of peptide bonds, and promotes the decomposition of aged keratin proteins. The microbial-derived protease is subtilisin, which has catalytic activity and substrate specificity, and degrades various impurities and toxin components composed of proteins and polypeptides on the skin surface.
[0008] Furthermore, the grape seed extract rich in proanthocyanidins contains multiple phenolic hydroxyl groups and has hydrogen-donating ability, and the green tea extract with heavy metal chelating ability contains tea polyphenol components, which contain phenolic hydroxyl groups and carboxyl functional groups. In the composition, the grape seed extract and the green tea extract act on the protease by adjusting the pH value of the system and reacting with free radicals, while their own activities interact with the protease to form a network.
[0009] Furthermore, the glycerol acts as a moisturizing agent, and its molecular structure contains multiple hydroxyl groups, which form hydrogen bonds with water molecules. On the skin surface, glycerol absorbs a large amount of water molecules by virtue of hydrogen bonds, forming a moisturizing film. On the one hand, this moisturizing film reduces the loss of moisture from the skin to the external environment, and on the other hand, provides a moist environment for the action of proteases on the skin surface, which is conducive to better contact and reaction between proteases and substrates on the skin surface.
[0010] Furthermore, the xanthan gum is used as a thickener, and its molecules are composed of glucose, mannose and glucuronic acid connected by glycosidic bonds to form a high molecular polymer. In the composition, the xanthan gum molecules interact with water molecules and other components, increasing the internal friction of the system by entanglement and wrapping, thereby adjusting the viscosity of the composition.
[0011] Furthermore, on the basis of the cross-source protease system, functional adjuvant system, moisturizer, thickener and solvent, the composition also adds less than 3% by weight of plant essential oil, which is extracted from the flowers, leaves, roots and seeds of the plant through distillation and pressing processes and is a volatile aromatic substance.
[0012] Furthermore, the preparation method of the composition comprises the following steps: Raw material pretreatment: dry papain and subtilisin at 35-40°C for 2-3 hours respectively, and grind grape seed extract and green tea extract into particle size of 80-100 mesh; Mixing of cross-source protease system: Weigh dried papain and subtilisin in proportion, place in a sterile stirring container, and stir at 100-150 rpm for 15-20 minutes; Mixing the functional excipient system: Weigh the crushed grape seed extract and green tea extract in proportion, add deionized water, and stir at 80-120 rpm in a 40-50°C water bath for 20-30 minutes to dissolve them; Initial mixing: slowly add the trans-source protease system to the functional adjuvant system solution and stir at 120-150 rpm for 30-40 minutes; Add other ingredients: glycerin, then xanthan gum, stirring at 150-200 rpm for 15-20 minutes. Volume adjustment and homogenization: Add deionized water to the required volume and use a homogenizer to homogenize at 2000-3000 rpm for 10-15 minutes.
[0013] Furthermore, in the raw material pretreatment step, papain and subtilisin are dried using vacuum drying technology with a vacuum degree of 0.05-0.08 MPa, and grape seed extract and green tea extract are crushed using low-temperature crushing technology, with the crushing process temperature controlled at 0-10°C.
[0014] Compared with the existing technology, this active protease composition for detoxification and skin purification has the following beneficial effects: The present invention accurately combines papain and subtilisin, which can not only decompose the protein in aged keratin, but also degrade various impurities and toxins, expand the range of decomposition substrates, and more comprehensively remove skin impurities and toxins. It uses grape seed extract and green tea extract in functional adjuvants to adjust the pH value of the system, eliminate free radicals, and enhance the stability of protease activity, ensuring that the product can effectively function in different environments. At the same time, with the help of the antioxidant, anti-inflammatory and chelating functions of the functional adjuvants, it can simultaneously repair skin inflammatory damage and resist environmental oxidative damage during the detoxification and purification process, providing comprehensive care for the skin.
[0015] Other advantages, objects and features of the present invention will be described in part in the following description and, in part, will be apparent to those skilled in the art based on an examination of the following or may be learned from the practice of the invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort.
[0017] Figure 1 The present invention is a schematic flow chart of a method for preparing an active protease composition for detoxification and skin purification. DETAILED DESCRIPTION
[0018] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in conjunction with the accompanying drawings and preferred embodiments.
[0019] An active protease composition for detoxification and skin purification, comprising the following components: The cross-source protease system consists of a plant-derived protease and a microbial-derived protease, wherein the plant-derived protease is papain and the microbial-derived protease is subtilisin, and the mass ratio of the plant-derived protease to the microbial-derived protease is 1:0.5-1.5; A functional adjuvant system comprising a grape seed extract rich in proanthocyanidins and a green tea extract with heavy metal chelating ability. The grape seed extract rich in proanthocyanidins contains multiple phenolic hydroxyl groups and has hydrogen-donating ability. The green tea extract with heavy metal chelating ability contains tea polyphenols, which contain phenolic hydroxyl groups and carboxyl functional groups. The mass ratio of the grape seed extract to the green tea extract is 1:0.8-1.2, and the mass ratio of the functional adjuvant system in the composition is 5%-15%. Moisturizer, thickener and solvent. The moisturizer is glycerin. Glycerin, as a moisturizer, accounts for 5%-10% by mass in the composition. Its molecular structure contains multiple hydroxyl groups, which form hydrogen bonds with water molecules. On the skin surface, glycerin absorbs a large amount of water molecules by hydrogen bonds to form a moisturizing film; the thickener is xanthan gum, which accounts for 0.5%-1.5% by mass in the composition. Xanthan gum, as a thickener, is composed of glucose, mannose and glucuronic acid connected by glycosidic bonds to form a high molecular polymer; the solvent is high-purity deionized water without impurity ions, and the balance is added.
[0020] On the basis of the cross-source protease system, functional adjuvant system, moisturizer, thickener and solvent, the composition also adds less than 3% of plant essential oil by weight. The plant essential oil is extracted from the flowers, leaves, roots and seeds of the plant through distillation and pressing processes, and is a volatile aromatic substance.
[0021] like Figure 1 As shown, the preparation method of the composition comprises the following steps: Raw material pretreatment: Papain and subtilisin are dried at 35-40°C for 2-3 hours respectively, using vacuum drying technology with a vacuum degree of 0.05-0.08 MPa. Grape seed extract and green tea extract are crushed to a particle size of 80-100 mesh using low-temperature crushing technology, and the crushing process temperature is controlled at 0-10°C; Mixing of cross-source protease system: Weigh dried papain and subtilisin in proportion, place in a sterile stirring container, and stir at 100-150 rpm for 15-20 minutes; Mixing the functional excipient system: Weigh the crushed grape seed extract and green tea extract in proportion, add deionized water, and stir at 80-120 rpm in a 40-50°C water bath for 20-30 minutes to dissolve them; Initial mixing: slowly add the trans-source protease system to the functional adjuvant system solution and stir at 120-150 rpm for 30-40 minutes; Add other ingredients: glycerin, then xanthan gum, stirring at 150-200 rpm for 15-20 minutes. Volume adjustment and homogenization: Add deionized water to the required volume and use a homogenizer to homogenize at 2000-3000 rpm for 10-15 minutes.
[0022] Example 1 To construct an efficient cross-source protease system, 10g of papain and 10g of subtilisin were accurately weighed. Papain is derived from plants, and its unique molecular structure enables it to act specifically on the protein structure in aging keratin of the skin; subtilisin comes from microorganisms and can degrade various impurities and toxin components on the skin surface. The two are combined in a 1:1 mass ratio. Research has verified that this ratio can fully utilize the advantages of the two proteases and broaden the scope of decomposition of impurities, toxins and aging keratin on the skin surface.
[0023] 10 g of proanthocyanidin-rich grape seed extract and 10 g of heavy metal-chelating green tea extract were weighed. Grape seed extract, due to its large amount of proanthocyanidins, has a strong antioxidant function; the components in green tea extract can effectively chelate heavy metal ions. The mass ratio of the two was 1:1. In this embodiment, they were formulated into a composition with a total mass of 100 g. The functional adjuvant system accounted for 10%, which is within the 5%-15% range determined by repeated experiments to be effective.
[0024] Accurately measure 8g of glycerin as a moisturizer. Glycerin molecules contain multiple hydroxyl groups, which can form hydrogen bonds with water molecules, adsorb and lock moisture on the skin surface. Its mass proportion of 8% is determined based on comprehensive consideration of the moisturizing effect and the impact on the stability of the overall composition. It can not only ensure good moisturizing performance, but also will not have adverse effects on other ingredients. Weigh 1g of xanthan gum as a thickener. Xanthan gum, as a high molecular polymer, can effectively adjust the viscosity of the composition. A proportion of 1% can make the composition have suitable fluidity and spreadability. At the same time, prepare sufficient deionized water as a solvent for dissolving and formulating other ingredients, and add the remainder to the final composition.
[0025] The preparation steps are as follows: Raw material pretreatment: To ensure the activity of the protease and the effect of the extract, the raw materials are pretreated. Papain and subtilisin are placed in a dry environment at 35°C and dried for 2 hours to remove moisture and stabilize the active structure of the protease. Grape seed extract and green tea extract are crushed to a particle size of 80 mesh using professional crushing equipment. The smaller particle size helps them dissolve and mix better in subsequent steps, fully exerting their efficacy.
[0026] Mixing of cross-source protease system: Carefully place the dried papain and subtilisin into a sterile stirring container, set the stirring speed to 100 rpm, and continue stirring for 15 minutes. During the stirring process, the two proteases are fully contacted and mixed, laying the foundation for subsequent synergistic action and forming a uniform cross-source protease system.
[0027] Mixing of functional excipient system: Add appropriate amount of deionized water to the crushed grape seed extract and green tea extract, place in a water bath at a constant temperature of 40°C, and stir at 80 rpm for 20 minutes. Under the combined action of heating and stirring in the water bath, the extract gradually dissolves to form a uniform functional excipient solution, ensuring that the active ingredients therein are fully dispersed.
[0028] Preliminary mixing: Slowly and evenly pour the prepared cross-source protease system into the functional adjuvant solution. During the pouring process, stir continuously with the stirring speed controlled at 120 rpm and the stirring time set to 30 minutes. This will allow the protease and functional adjuvant to be initially mixed evenly, promote the interaction between them, and build the basic framework for synergistic effect.
[0029] Add other ingredients: Add the weighed glycerin and xanthan gum to the preliminary mixed solution in order. During the addition process, maintain a stirring speed of 150 rpm for 15 minutes. Through continuous stirring, the glycerin and xanthan gum can be evenly dispersed in the solution and fully integrated with other ingredients to jointly shape the characteristics of the composition.
[0030] Volume setting and homogenization: Deionized water is slowly added to the mixed solution until the total mass of the solution reaches 100 g to complete the volume setting operation. Subsequently, a homogenizer is used to homogenize at a speed of 2000 rpm for 10 minutes. The homogenization process makes the texture of the entire composition more uniform and delicate, ensuring that the various ingredients are fully mixed at the microscopic level, improving the stability and use effect of the product, and ultimately obtaining a high-quality active protease composition that detoxifies and purifies the skin.
[0031] Example 2 In this example, to further explore the optimal ratio of the cross-source protease system, 12g of papain and 8g of subtilisin were accurately weighed. Papain is derived from plants and has a specific molecular structure that can accurately identify and bind to proteins in aging skin keratin, promoting its decomposition through catalysis; subtilisin comes from microorganisms, and its unique catalytic activity can degrade various impurities and toxins composed of proteins, peptides, etc. on the skin surface. This 1.5:1 mass ratio is based on a large amount of previous experimental data and theoretical analysis to determine the exploration direction, aiming to observe the difference in the decomposition effect of the two proteases working together at this ratio on skin impurities, toxins and aging keratin.
[0032] Carefully measure 12g of grape seed extract rich in proanthocyanidins and 8g of green tea extract with heavy metal chelating ability. Grape seed extract is rich in proanthocyanidins, which have multiple phenolic hydroxyl structures, which give it a strong hydrogen supply ability, thereby effectively scavenging free radicals; tea polyphenols and other ingredients in green tea extract contain functional groups such as phenolic hydroxyl and carboxyl, which can undergo chelation reaction with heavy metal ions. The mass ratio of the two is 1.5:1. When the composition with a total mass of 100g is prepared, the functional adjuvant system accounts for 20%, which is close to the upper limit of its effective proportion range, so as to study the effect of a high proportion of functional adjuvants on the performance of the overall composition.
[0033] Accurately measure 7g of glycerol as a moisturizer. The multiple hydroxyl groups in the glycerol molecule enable it to form a large number of hydrogen bonds with water molecules, adsorbing and locking moisture on the skin surface. After many experiments, it has been verified that a mass proportion of 7% can not only provide good moisturizing effect for the skin, but also will not affect the stability of the composition or cause discomfort to the skin due to excessive concentration. Weigh 1.2g of xanthan gum as a thickener. Xanthan gum is a high molecular polymer composed of glucose, mannose and glucuronic acid connected by specific glycosidic bonds. A proportion of 1.2% can effectively adjust the viscosity of the composition, so that it has appropriate fluidity and adhesion when applied, so as to facilitate uniform coverage of the skin surface. At the same time, prepare sufficient deionized water as a solvent to dissolve and formulate other ingredients, and add the remainder to the final composition.
[0034] The preparation steps are as follows: Raw material pretreatment: To ensure that each raw material can fully play its role in the subsequent preparation process, the raw materials are carefully pretreated. Papain and subtilisin are placed in a dry environment at 38°C and dried for 2.5 hours. This temperature and time setting can not only effectively remove moisture from the protease, but also protect its active structure to the greatest extent, avoiding loss of activity due to over-drying or high temperature. Using professional crushing equipment, grape seed extract and green tea extract are crushed to a particle size of 90 mesh. The smaller particle size can increase the specific surface area of the extract, so that it can contact with the solvent faster and more fully in the subsequent dissolution process, improve the dissolution efficiency, and thus ensure that its active ingredients can be evenly dispersed in the composition.
[0035] Mixing of cross-source protease system: Carefully place the dried papain and subtilisin into a strictly disinfected sterile stirring container, set the stirring speed to 120 rpm, and continue stirring for 18 minutes. During the stirring process, the two proteases gradually come into full contact and mix, and the interaction between the molecules gradually increases, laying a solid foundation for the subsequent synergistic effect in the skin care process, and finally forming a uniform and stable cross-source protease system.
[0036] Mixing of functional adjuvant system: Add appropriate amount of deionized water to the crushed grape seed extract and green tea extract, place in a water bath at a constant temperature of 45°C, and stir at 100 rpm for 25 minutes. Under the dual effects of water bath heating and stirring, the extract gradually dissolves. The water bath temperature of 45°C can not only accelerate the dissolution process of the extract, but also avoid the destruction of the active ingredients therein due to excessive temperature. Continuous stirring can make the dissolved extract molecules evenly dispersed in the solution, forming a uniform and stable functional adjuvant solution, ensuring that the active ingredients such as antioxidants and heavy metal complexes are fully dispersed, preparing for subsequent mixing with the protease system.
[0037] Preliminary mixing: Slowly and evenly pour the prepared cross-source protease system into the functional adjuvant solution. During the pouring process, stir continuously with the stirring speed controlled at 130 rpm and the stirring time set to 35 minutes. The slow pouring method allows the protease system to fully contact with the functional adjuvant solution, avoiding local excessive concentration or uneven mixing caused by pouring too fast. Continuous stirring helps promote the interaction between the protease and the functional adjuvant, allowing them to begin to build a basic framework for synergistic effect and initially form an interconnected system.
[0038] Add other ingredients: Add the weighed glycerin and xanthan gum to the preliminary mixed solution in order. During the addition process, maintain a stirring speed of 180 rpm for 18 minutes. Rapid stirring can quickly disperse the glycerin and xanthan gum in the solution and fully blend with other ingredients. The addition of glycerin further enhances the moisturizing properties of the composition, while the xanthan gum begins to play its role in regulating viscosity, so that the composition gradually reaches a suitable texture, preparing for subsequent volume setting and homogenization operations.
[0039] Volume setting and homogenization: Slowly add deionized water to the mixed solution until the total mass of the solution reaches 100g to complete the volume setting operation. During the volume setting process, the amount of deionized water added needs to be precisely controlled to ensure that the proportions of each component in the composition are accurate. Subsequently, use a homogenizer to homogenize at a speed of 2500 rpm for 12 minutes. High-speed homogenization can make the texture of the entire composition more uniform and delicate. At the microscopic level, the various components are fully mixed, and the interactions between molecules are more stable, thereby improving the stability and use effect of the product, and ultimately obtaining an active protease composition with excellent quality and stable performance for detoxification and skin purification.
[0040] Example 3 In this example, 8 g of papain and 12 g of subtilisin were selected to construct a cross-source protease system with a mass ratio of 2:3 between plant-derived protease and microbial-derived protease. The selection of this ratio was based on in-depth research on the synergistic mechanism of proteases and further exploration of previous experimental results. The unique structures and catalytic properties of papain and subtilisin make them have significant differences in skin care effects at different ratios. By adjusting the ratio of the two, the aim is to explore a more optimized combination to achieve a more efficient decomposition of skin impurities, toxins and aging keratin.
[0041] 8 g of grape seed extract rich in proanthocyanidins and 12 g of green tea extract with heavy metal chelating ability were accurately measured, with the same mass ratio of 2:3. In 100 g of the composition, the functional adjuvant system accounted for 20%, the same as the proportion of the functional adjuvant system in Example 2. However, different raw material ratios may affect the synergistic effect with the protease system. The antioxidant function of the grape seed extract and the heavy metal chelating ability of the green tea extract may interact with the protease in different ways and degrees at different ratios. This will provide new data and reference for studying the performance of the overall composition.
[0042] Prepare 9g of glycerin as a moisturizer, accounting for 9%. Glycerin can tightly combine with water molecules due to the multiple hydroxyl groups in its molecular structure, forming a moisturizing barrier on the skin surface to reduce water loss. The 9% proportion has been verified by many experiments. While ensuring good moisturizing effect, it will not have a negative impact on other properties of the composition. Weigh 0.8g of xanthan gum as a thickener, accounting for 0.8%. The high molecular structure of xanthan gum enables it to form a three-dimensional network structure in the composition, effectively adjusting the viscosity of the composition. The amount of 0.8% can make the composition have a moderate viscosity, which is convenient for application and can ensure its adhesion to the skin. Prepare sufficient deionized water as a solvent for dissolving and formulating other ingredients, and add the remainder to the final composition.
[0043] The preparation steps are as follows: Raw material pretreatment: To ensure the quality and activity of the raw materials, they are strictly pretreated. Papain and subtilisin are dried at 40°C for 3 hours. The higher temperature and longer drying time can remove moisture more thoroughly. At the same time, advanced drying technology and equipment are used to ensure that the activity of the protease is not affected. Professional crushing equipment is used to crush the grape seed extract and green tea extract to a particle size of 100 mesh. The extremely fine particle size can significantly improve the solubility and dispersibility of the extract, so that it can be better integrated with other ingredients in the subsequent preparation process to give full play to its efficacy.
[0044] Mixing of cross-source protease system: Place dried papain and subtilisin into a sterile stirring container and stir at 150 rpm for 20 minutes. High-speed stirring enables the two proteases to mix quickly and thoroughly, promoting intermolecular interactions and forming a stable cross-source protease system. During the stirring process, closely monitor the mixing state to ensure uniform distribution of the proteases, providing a good foundation for subsequent reactions.
[0045] Mixing of functional adjuvant system: Add appropriate amount of deionized water to the crushed grape seed extract and green tea extract, and stir at 120 rpm in a 50°C water bath for 30 minutes. The 50°C water bath temperature can accelerate the dissolution process of the extract while maintaining the stability of its active ingredients. Rapid stirring can make the extract quickly disperse in the solution to form a uniform functional adjuvant solution, creating favorable conditions for subsequent mixing with the protease system.
[0046] Preliminary mixing: Slowly pour the cross-source protease system into the functional excipient solution and stir at 150 rpm for 40 minutes. Slow pouring and continuous high-speed stirring help the two systems to fully contact and mix, promote the interaction between the protease and the functional excipient, and form a preliminary synergistic system. During the stirring process, observe the changes in the solution to ensure uniform mixing and prepare for the subsequent addition of other ingredients.
[0047] Adding other ingredients: glycerin and xanthan gum are added to the preliminary mixed solution in sequence, with the stirring speed controlled at 200 rpm for 20 minutes. High-speed stirring enables the glycerin and xanthan gum to be quickly and evenly dispersed in the solution and fully integrated with other ingredients. The addition of glycerin enhances the moisturizing properties of the composition, while the xanthan gum further adjusts the viscosity of the composition to achieve an ideal state.
[0048] Volume setting and homogenization: Deionized water is added to the mixed solution to a total mass of 100 g to complete the volume setting operation. High-precision weighing equipment and measuring instruments are used during the volume setting process to ensure the accuracy of the solution quality. Subsequently, a homogenizer is used to homogenize at a speed of 3000 rpm for 15 minutes. High-speed homogenization can make the texture of the entire composition more uniform and delicate, achieve sufficient mixing of the various ingredients at the microscopic level, improve the stability and consistency of the product, and ultimately obtain an active protease composition with excellent performance in detoxification and skin purification.
[0049] Comparative Example This comparative example uses only 20g of papain as the source of protease in the entire formula. Papain was chosen because it is relatively common in existing skin care products and is often used to soften and clean the stratum corneum. However, in this comparative example, it is not compounded with other proteases in order to compare the synergistic effect of the cross-source protease system.
[0050] No natural active ingredients such as grape seed extract and green tea extract with antioxidant, anti-inflammatory and heavy metal chelating functions are added, which is significantly different from the functional adjuvant system in the examples. The purpose is to observe the impact of the lack of these ingredients on the overall performance of the product.
[0051] Propylene glycol is selected as a moisturizer instead of glycerin, and the mass proportion is set to 8%. Propylene glycol is a commonly used moisturizer with a certain moisture absorption capacity and is widely used in many skin care products. However, its moisturizing principle and effect are different from those of glycerin. Glycerin absorbs and locks moisture by forming hydrogen bonds with water molecules, while the moisturizing mechanism of propylene glycol is relatively simple, mainly relying on its own hydrophilicity to maintain a certain amount of moisture.
[0052] 1g of sodium carboxymethyl cellulose was used instead of xanthan gum as a thickener. Sodium carboxymethyl cellulose is a common water-soluble polymer compound that can increase the viscosity of the solution and is widely used in food, cosmetics and other fields. Compared with xanthan gum, sodium carboxymethyl cellulose differs in the formed colloidal structure, the effect on the system stability and the interaction with other ingredients.
[0053] As in the embodiment, deionized water is selected as the solvent and the balance is added. Deionized water has the characteristics of high purity and no impurity ions, and can provide a stable dissolution environment for other ingredients, ensuring the stability of the formula system.
[0054] The preparation steps are as follows: Initial dissolution: Slowly add 20g of papain into an appropriate amount of deionized water and stir at room temperature (25°C) at a stirring speed of 100 rpm until the papain is completely dissolved. During this process, closely observe the dissolution of the protease to ensure that it is fully dispersed in the water to avoid agglomeration or incomplete dissolution.
[0055] Add moisturizer and thickener: After papain is completely dissolved, add 8g of propylene glycol and continue stirring for 5 minutes to evenly disperse the propylene glycol in the solution. Then, slowly sprinkle 1g of sodium carboxymethyl cellulose into the solution while stirring. Increase the stirring speed to 150 rpm and set the stirring time to 20 minutes. When adding sodium carboxymethyl cellulose, pay attention to its addition speed to avoid the formation of lumps in the solution, which will affect the thickening effect and product quality.
[0056] Volume adjustment and homogenization: After stirring, add deionized water to the solution and adjust the volume to 100g. Use a homogenizer to homogenize at a speed of 2000 rpm for 10 minutes to make the entire system more uniform and stable. The homogenization process can make the various components in the solution more evenly distributed at the microscopic level, improve the stability and consistency of the product, and reduce the occurrence of stratification or precipitation.
[0057] During the preparation process of the comparative example, the composition properties thereof were different from those of the examples, resulting in different product properties. The specific parameter differences are shown in the following table:
[0058] As shown in the table above, comprehensive comparison shows that the examples all use compound formulas, and the proteases and functional adjuvants work together to achieve diverse performances in cleaning and skin care. Different ratios of proteases and adjuvants bring different cleaning focuses and skin care efficacy differences. The comparative examples have a single ingredient and lack synergy, and their cleaning and skin care effects are far inferior to those of the examples. The example formulas have more advantages in comprehensive skin care.
[0059] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.
Claims
1. An active protease composition for detoxification and skin purification, characterized in that: The composition includes the following components: A cross-source protease system, consisting of a plant-derived protease and a microbial-derived protease, wherein the mass ratio of the plant-derived protease to the microbial-derived protease is 1:0.5-1.5; A functional adjuvant system comprising a grape seed extract rich in proanthocyanidins and a green tea extract with heavy metal chelating ability, wherein the mass ratio of the grape seed extract to the green tea extract is 1:0.8-1.2, and the mass ratio of the functional adjuvant system in the composition is 5%-15%; A humectant, a thickener and a solvent, wherein the humectant is glycerin, which accounts for 5% to 10% by weight of the composition; the thickener is xanthan gum, which accounts for 0.5% to 1.5% by weight of the composition; and the solvent is deionized water of high purity and free of impurity ions, with the remainder added.
2. The active protease composition for detoxification and skin purification according to claim 1, characterized in that: The plant-derived protease is papain, and the microbial-derived protease is subtilisin.
3. The active protease composition for detoxification and skin purification according to claim 1, characterized in that: The grape seed extract rich in proanthocyanidins contains multiple phenolic hydroxyl groups and has hydrogen-donating ability. The green tea extract with heavy metal complexing ability contains tea polyphenol components, including phenolic hydroxyl groups and carboxyl functional groups.
4. The active protease composition for detoxification and skin purification according to claim 1, characterized in that: The glycerin acts as a moisturizing agent, and its molecular structure contains multiple hydroxyl groups, which form hydrogen bonds with water molecules. On the surface of the skin, glycerin absorbs a large amount of water molecules by means of hydrogen bonds, forming a moisturizing film.
5. The active protease composition for detoxification and skin purification according to claim 1, characterized in that: The xanthan gum is used as a thickener, and its molecules are formed by glucose, mannose and glucuronic acid connected by glycosidic bonds to form a high molecular polymer.
6. The active protease composition for detoxification and skin purification according to claim 1, characterized in that: On the basis of the cross-source protease system, functional adjuvant system, moisturizer, thickener and solvent, the composition also adds less than 3% by weight of plant essential oil, which is extracted from the flowers, leaves, roots and seeds of plants through distillation and pressing processes and is a volatile aromatic substance.
7. The active protease composition for detoxification and skin purification according to claim 1, characterized in that: The preparation method of the composition comprises the following steps: Raw material pretreatment: dry papain and subtilisin at 35-40°C for 2-3 hours respectively, and grind grape seed extract and green tea extract into particle size of 80-100 mesh; Mixing of cross-source protease system: Weigh dried papain and subtilisin in proportion, place in a sterile stirring container, and stir at 100-150 rpm for 15-20 minutes; Mixing the functional excipient system: Weigh the crushed grape seed extract and green tea extract in proportion, add deionized water, and stir at 80-120 rpm in a 40-50°C water bath for 20-30 minutes to dissolve them; Initial mixing: slowly add the trans-source protease system to the functional adjuvant system solution and stir at 120-150 rpm for 30-40 minutes; Add other ingredients: glycerin, then xanthan gum, stirring at 150-200 rpm for 15-20 minutes. Volume adjustment and homogenization: Add deionized water to the required volume and use a homogenizer to homogenize at 2000-3000 rpm for 10-15 minutes.
8. The active protease composition for detoxification and skin purification according to claim 7, characterized in that: In the raw material pretreatment step, papain and subtilisin are dried using vacuum drying technology with a vacuum degree of 0.05-0.08 MPa, and grape seed extract and green tea extract are crushed using low-temperature crushing technology with the crushing process temperature controlled at 0-10°C.