Method for controlling clubroot

Abstract

Provided is a method for controlling clubroot in soil in which clubroot of brassicaceous vegetables is frequently occurring due to the presence of pratylenchus pratensis. The invention relates to a method for preventing and removing clubroot of cruciferous vegetables. The clubroot of the cruciferous vegetables is prevented and removed by applying (a) a bactericide and (b) a nematicide in soil in which pratylenchus pratensis is generated.

Description

Technical Field

[0001] The present invention relates to a method for preventing and controlling clubroot of cruciferous vegetables by applying a fungicide and a nematicide to soil where clubroot of cruciferous vegetables is frequently caused by the presence of Pratylenchus nematodes. Background Art

[0002] Clubroot of cruciferous vegetables is a soil-borne plant disease caused by the pathogen Plasmodiophora brassicae. The pathogen parasitizes the roots of cruciferous vegetables such as cabbage, Chinese cabbage, and turnips, causing abnormal proliferation of infected root cells, resulting in swellings of various sizes. The formation of these swellings hinders the absorption of water and nutrients, reducing crop yield and quality and sometimes causing the crops to wither and die. Furthermore, because the pathogen forms large numbers of dormant spores in infected tissues, these dormant spores can survive in the soil for long periods of time, making it an extremely difficult disease to control.

[0003] Among the nematodes that parasitize plants, three species pose agricultural problems: root-knot nematodes, Pratylenchus nematodes, and cyst nematodes. Pratylenchus nematodes, in particular, have the property of invading plants while moving, then escaping and re-entering the plant from another location, repeating this pattern of movement and invading. They are known to cause browning, necrosis, and deformities at the site of invasion, severely damaging crop yields and quality. They can also spread to soil pathogens through the site of invasion, further exacerbating the damage.

[0004] Patent Document 1 describes a composition composed of an organophosphorus insecticide and a fungicide that can control pests and diseases that inhabit soil and seeds. In particular, applying a composition composed of thiazolyl and fluazinam to soil contaminated with southern root-knot nematodes and Fusarium spp. slightly suppresses the onset of cucumber wilt. Furthermore, it is described that applying dazomet to the same contaminated soil, followed by degassing and then applying thiazolyl, eliminates nematode root knots and suppresses wilt. However, there are no specific descriptions of controlling clubroot in cruciferous vegetables in soil containing Pratylenchus nematodes.

[0005] Patent Document 2 describes that seed application with imidazole fungicides such as fenvalerate and cyazofamid and / or a combination of fluazinam and carbosulfan exhibits excellent efficacy against clubroot in Chinese cabbage. However, there is no specific description of the control of clubroot in cruciferous vegetables in soils containing Pratylenchus nematodes.

[0006] Patent Document 3 describes a composition composed of fluazinam and fluopyram, and Patent Document 4 describes a composition composed of fluazinam and thiazolinone, which exhibit synergistic effects against root rot and nematodes in peanuts, peppers, and other plants, with prolonged residual effects. However, there are no specific descriptions regarding the control of clubroot in cruciferous vegetables in soils containing Pratylenchus nematodes.

[0007] Patent Document 5 describes that a composition composed of fluazinam and thiazolinone can be applied to rice seeds to control bakanae disease and heartworm nematodes. However, there is no specific description regarding the control of Pratylenchus nematodes or clubroot disease of cruciferous vegetables.

[0008] Patent Document 6 describes a composition containing chlorantraniliprole, indoxasulfamide, and lambda-cyhalothrin as effective against Chinese cabbage clubroot, and a composition containing chlorantraniliprole, indoxasulfamide, and abamectin as effective against tomato root-knot nematodes. However, there is no specific description of the control of clubroot in cruciferous vegetables in soils containing Pratylenchus nematodes.

[0009] Non-Patent Document 1 describes that the control effect of Chinese cabbage clubroot is enhanced when fluazinam and thiamethoxam are applied simultaneously to soil contaminated with clubroot fungi and Pratylenchus penetrans.

[0010] Prior art literature

[0011] Patent Literature

[0012] Patent Document 1: International Publication No. 2005 / 070206

[0013] Patent Document 2: Chinese Patent Application Publication No. 103461349

[0014] Patent Document 3: Chinese Patent Application Publication No. 105594705

[0015] Patent Document 4: Chinese Patent Application Publication No. 105851054

[0016] Patent Document 5: Chinese Patent Application Publication No. 105918346

[0017] Patent Document 6: Chinese Patent Application Publication No. 108541710

[0018] Non-patent literature

[0019] Non-Patent Literature 1: Nanami Kuwahara et al., (2023) Proceedings of the 5th Annual Meeting of the Japanese Society of Plant Pathology, p. 97 Summary of the Invention

[0020] Problems to be solved by the invention

[0021] As mentioned above, Pratylenchus nematodes repeatedly invade, infect, escape, and reinvade plants, leaving numerous traces of their presence. Consequently, soil diseases are prone to frequent occurrence in soils where Pratylenchus nematodes are present. In particular, the simultaneous occurrence of clubroot in cruciferous vegetables can cause significant damage to crops, necessitating effective control measures.

[0022] Means for solving problems

[0023] The present inventors have conducted intensive studies to solve the above-mentioned problems and have found that the combined use of (a) a fungicide and (b) a nematicide provides an unexpectedly superior control effect compared to the use of each component alone, thereby completing the present invention.

[0024] Specifically, the present invention relates to a method for controlling clubroot disease in cruciferous vegetables in soil where Pratylenchus nematodes exist, comprising applying (a) a fungicide and (b) a nematicide to control clubroot disease.

[0025] The present invention also relates to a method for controlling clubroot of cruciferous vegetables in soil where Pratylenchus nematodes are present, wherein a composition is applied to seeds or seedlings of cruciferous vegetables, cultivation soil, or cultivation fields, wherein the composition is characterized in that it contains (a) a fungicide and (b) a nematicide as active ingredients.

[0026] The present invention also relates to a method for controlling clubroot of cruciferous vegetables in soil where Pratylenchus nematodes are present, wherein (a) a fungicide and (b) a nematicide are combined and applied to cultivation soil, seeds, seedlings, or cultivation fields of the cruciferous vegetables.

[0027] Effects of the Invention

[0028] The present invention suppresses the damage of Pratylenchus nematodes even in soil where Pratylenchus nematodes exist, thereby stably achieving a high control effect on clubroot of cruciferous vegetables. Therefore, the present invention is useful for controlling clubroot of cruciferous vegetables.

[0029] Furthermore, in the present invention, since the combined use of (a) a fungicide and (b) a nematicide provides an unexpectedly superior control effect compared to the case where each component is used alone, the application amount of each component can be reduced, and thus the combination can be used on plants whose growth is affected by the usual application amount. DETAILED DESCRIPTION

[0030] As the fungicide component (a) of the present invention (hereinafter, also referred to as component (a)), preferably fluazinam, cyazofamid, sulfaquinoxaline and indazolesulfaquinoxaline, more preferably fluazinam, indazolesulfaquinoxaline and sulfaquinoxaline, and further preferably fluazinam. The above components (a) can be used alone or in combination of two or more.

[0031] The nematicide of component (b) of the present invention (hereinafter also referred to as component (b)) is preferably thiabendazole, cadasifos, cypermethrin, carbosulfan, oxamyl, fluopyram, fluthiapyr, triflupyramide, and abamectin; more preferably thiabendazole, cadasifos, cypermethrin, carbosulfan, oxamyl, and fluopyram; further preferably thiabendazole, cadasifos, and cypermethrin; and even more preferably thiabendazole. Component (b) may be used alone or in combination of two or more.

[0032] Components (a) and (b) of the present invention may be in the form of salts. Examples of the salts include those acceptable in the field of pesticides, such as alkali metal salts such as sodium salts and potassium salts; alkaline earth metal salts such as magnesium salts and calcium salts; ammonium salts such as dimethylammonium salts and triethylammonium salts; inorganic acid salts such as hydrochlorides, perchlorates, sulfates, and nitrates; and organic acid salts such as acetates, trifluoroacetates, oxalates, p-toluenesulfonates, and methanesulfonates.

[0033] In the present invention, components (a) and (b) may be prepared separately and mixed at the time of application. In this case, commercially available formulations of each may be used. When mixed at the time of application, for example, the formulations may be diluted with water in a spray tank and mixed simultaneously. Furthermore, in the present invention, components (a) and (b) may be prepared together and applied.

[0034] In the present invention, component (a) and / or component (b) can be mixed with various auxiliary formulation ingredients as in conventional pesticide formulations to form various formulations such as emulsions, powders, fine particles, granules, wettable powders, water-dispersible granules, aqueous suspensions, oily suspensions, liquids (liquids), and pastes. As long as they are suitable for the purpose of the present invention, they can be any formulation form commonly used in this field. Regarding the mixing ratio relative to the entire formulation, the auxiliary formulation ingredients are usually 1 to 99.999 parts by weight relative to 0.001 to 99 parts by weight of component (a) and / or component (b) (hereinafter also referred to as the active ingredient), preferably 5 to 99.99 parts by weight relative to 0.01 to 95 parts by weight of the active ingredient, and more preferably 20 to 99.99 parts by weight relative to 0.01 to 80 parts by weight of the active ingredient. When these formulations are actually used, they can be used directly or diluted with a diluent such as water to a specified concentration.

[0035] As auxiliary ingredients of the preparation, there can be mentioned plant or animal powders such as starch, activated carbon, soybean flour, wheat flour, wood flour, fish meal, milk powder, etc.; mineral powders such as talc, kaolin, bentonite, calcium carbonate, zeolite, diatomaceous earth, white carbon, clay, aluminum oxide, etc.; sulfur powder; solid carriers such as anhydrous sodium sulfate, in addition, there can be mentioned water; alcohols such as methanol and ethylene glycol; ketones such as acetone, methyl ethyl ketone, N-methyl-2-pyrrolidone, etc.; diols such as ethanol and ethylene glycol; Ethers such as alkanes and tetrahydrofuran; aliphatic hydrocarbons such as kerosene and lamp oil; aromatic hydrocarbons such as xylene, trimethylbenzene, tetramethylbenzene, cyclohexane, and solvent naphtha; halogenated hydrocarbons such as chloroform and chlorobenzene; acid amides such as dimethylformamide; esters such as ethyl acetate and glycerides of fatty acids; nitriles such as acetonitrile; sulfur-containing compounds such as dimethyl sulfoxide; and liquid carriers such as vegetable oils such as soybean oil and rapeseed oil. Emulsifiers, suspending agents, dispersants, surfactants, penetrants, wetting agents, thickeners, defoaming agents, stabilizers, antifreeze agents, etc. can be added as needed.

[0036] The application in the present invention can be carried out by spreading (e.g., spraying, misting, atomizing, granulation, water surface application, etc.), soil spreading (mixing, pouring, etc.), surface application (painting, dusting, covering, etc.), etc., which are generally used in the field of pesticides. In addition, application can also be carried out by application using an aerial device such as a remote-controlled unmanned aircraft or an unmanned helicopter, or by so-called ultra-low volume application. In addition, component (a) and component (b) can be applied separately as separately formulated substances, or can be applied as a mixture, or can be applied as a substance formulated together. The formulated substance can be applied directly or diluted with water or the like.

[0037] The application in the present invention can include soil treatment such as soil spreading, soil mixing, soil irrigation, planting furrow (planting ditch) spreading, planting furrow soil spreading, planting furrow soil mixing, ridge (strip) soil mixing; seed treatment such as seed coating and seed powder coating; seedling treatment such as seedling immersion, seedbed irrigation, and cell tray irrigation; planting hole treatment such as planting hole spreading and planting hole irrigation treatment; and other general methods used by those skilled in the art, but soil treatment is preferred, and soil mixing is preferred among them.

[0038] The active ingredient of the present invention is effective not only for controlling clubroot of cruciferous vegetables, but also for nematodes such as Pratylenchus penetrans, Pratylenchus coffeae, Pratylenchus neglectus, Pratylenchus brachyurus, and Radopholus similis, and for nematodes of the family Pratylenchidae; Meloidogyne hapla, Meloidogyne incognita, Meloidogyne javanica, and guava root-knot nematodes (Meloidogyne enterolobii); and Globodera spp. rostochiensis), Globodera pallida, Heterodera glycines, etc.; Hoplolaimidae, such as Rotylenchulus reniformis; Aphelenchoididae, such as Aphelenchoides besseyi; Anguinidae, such as Nothotylenchus acris and Ditylenchus dipsaci; Tylenchulus semi-penetrating semipenetrans); Longidoridae such as Xiphinemaindex; Trichodoridae; Parasitaphelenchidae such as Bursaphelenchusxylophilus; etc. are also effectively controlled. Among them, the Braybowl family is preferred, and the penetrating Braybowl nematode and the coffee Braybowl nematode are particularly preferred.

[0039] Examples of cruciferous vegetables to be controlled for clubroot in the present invention include turnips, Chinese cabbage, non-heading cabbage, cabbage, broccoli, cauliflower, rapeseed (Abrana), rapeseed (Nabanana), rapeseed (Nataneh), daikon (Daikon), Kyoto mizuna (Kowona), komatsuna (Komatsuna), takana (Takana), mizuna (Mizuna), Mibunaga (Mibunaga), nozawa (Nozawana), mizuna (Mizukakena), kale, and choy sum (Chicken lettuce). The present invention relates to a kind of vegetable and vegetable that is preferably selected from the group consisting of turnip, broccoli, mustard greens, mustard greens, mustard tubers, mustard greens, Chinese cabbage, wasabi, horseradish, etc., preferably turnip, Chinese cabbage, non-heading cabbage, cabbage, broccoli, cauliflower, rapeseed, rapeseed, white radish, Kyoto mizuna, komatsuna, takana, mizuna, Mizuna, Nozawa, water spinach, kale, green onion, radish, Brussels sprouts, wasabi, among which Chinese cabbage, cabbage, broccoli, cauliflower, mizuna are more preferred.

[0040] The mixing ratio of component (a) to component (b) in the present invention is 1:1,000 to 1,000:1 by weight, preferably 1:100 to 100:1, more preferably 1:10 to 10:1, and even more preferably 1:10 to 5:1.

[0041] The application rate of the active ingredient of the present invention varies depending on the meteorological conditions, target crops, method of use, formulation form, etc., and cannot be generally specified. For example, when applying by soil spraying, soil mixing, furrow soil mixing, planting furrow spraying, in-furrow soil spraying, and planting furrow soil mixing, the application rate of component (a) as the active ingredient is generally 0.1 to 10,000 g / ha, preferably 1 to 5,000 g / ha, and more preferably 500 g / ha to 2,500 g / ha, and the application rate of component (b) is generally 0.1 to 10,000 g / ha, preferably 1 to 10,000 g / ha, and more preferably 1,000 g / ha to 6,000 g / ha. When applying by soil injection, the concentration of component (a) is generally 0.1 to 10,000 ppm, preferably 1 to 4,000 ppm, and the concentration of component (b) is generally 0.1 to 10,000 ppm, preferably 1 to 4,000 ppm. When spraying into planting holes, the application amount of component (a) is generally 0.01 to 500 g / hole, preferably 0.1 to 100 g / hole, and the application amount of component (b) is generally 0.01 to 500 g / hole, preferably 0.1 to 100 g / hole. When applying to seeds and seedlings, the application amount of component (a) is generally 0.25 to 500 g / hole, preferably 10 to 200 g / hole, and the application amount of component (b) is generally 0.25 to 500 g / hole, preferably 10 to 200 g / hole. The application amounts of these can be appropriately changed depending on the occurrence of Pratylenchus nematodes and clubroot disease.

[0042] In the present invention, in addition to the active ingredients of the present invention, other pesticides such as fungicides, insecticides, acaricides, nematicides, soil pesticides, antivirals, attractants, herbicides, plant growth regulators, etc. can also be further combined for treatment.

[0043] The active ingredient compound of the fungicide in the above-mentioned other pesticides can be appropriately selected from the following compound group. Even if not specifically stated, when these compounds exist in various structural isomers such as salts, alkyl esters, and optical isomers, these are of course also included.

[0044] Anilinopyrimidine compounds such as mepanipyrim, pyrimethanil, and cyprodinil; Triazolopyrimidine compounds such as ametoctradin; Triazolobenzothiazole compounds such as tricyclazole; triadimefon, bitertanol, triflumizole, etaconazole, propiconazole, penconazole, flusilazole, myclobutanil, cyproconazole, tebuconazole, hexaconazole, furconazole-cis, prochloraz, metconazole, epoxiconazole, tetraconazole, Azoles such as oxpoconazole fumarate, prothioconazole, triadimenol, flutriafol, difenoconazole, fluquinconazole, fenbuconazole, bromuconazole, diniconazole, simeconazole, pefurazoate, ipconazole, imibenconazole, azaconazole, triticonazole, imazalil, ipfentrifluconazole, mefentrifluconazole, and fluoxytioconazole; Quinoxaline compounds such as chinomethionat; Dithiocarbamate compounds such as maneb, zineb, mancozeb, polycarbamate, metiram, propineb, and thiram; Organochlorine compounds such as tetrachlorophthalide, chlorothalonil, and pentachloronitrobenzene; Benzimidazole compounds such as benomyl, thiophanate-methyl, carbendazim, thiabendazole, and fuberidazole; Cyanoacetamide compounds such as cymoxanil; Metalaxyl, Metalaxyl-M (also known as Mefenoxam), Phenylamide compounds such as oxadixyl, ofurace, benalaxyl, benalaxyl-M (also known as kiralaxyl, chiralaxyl), furalaxyl, and valifenalate; Anilide compounds such as cyprofuram, carboxin, oxycarboxin, thifluzamide, boscalid, fenhexamid, isotianil, tiadinil, and pyraziflumid; Sulfonamide (sulfenic acid) compounds such as dichlofluanid and tolylfluanid; Copper compounds such as cupric hydroxide, oxine copper, anhydrous copper sulfate, copper nonylphenolsulfonate, 8-hydroxyquinoline copper, and dodecylbenzenesulfonic acid bis(ethylenediamine)copper complex (II) (also known as DBEDC); Organophosphorus compounds such as fosetyl-Al, tolclofos-methyl, edifenphos, and iprobenfos; Phthalimide compounds such as captan, captafol, and folpet; Dicarboximide compounds such as procymidone, iprodione, and vinclozolin; Flutolanil, mepronil, benodanil, flutolanil Benzanilide compounds such as flufenoxadiazam; Amide compounds such as carpropamid, diclocymet, silthiofam, and fenoxanil; Pyrazole carboxamide compounds such as benzovindiflupyr, bixafen, fluindapyr, fluxapyroxad, furametpyr, isopyrazam, penflufen, penthiopyrad, pydiflumetofen, sedaxane, isoflucypram, inpyrfluxam, pyrapropoyne, and flubeneteram; Benzamide compounds such as fluopicolide, zoxamide, and fluopimomide; Furananiline compounds such as fenfuram; Thiophene amide compounds such as isofetamidopropynil (isofetamid); Piperazine compounds such as triforine; Pyrifenox, pyrifenox Pyridine compounds such as pyrisoxazole and aminopyrifen; Pyrimidine compounds such as fenarimol, ferimzone, nuarimol, and flumetylsulforim; Piperidine compounds such as fenpropidin; Morpholine compounds such as fenpropimorph and tridemorph; Organotin compounds such as triphenyltin hydroxide and triphenyltin acetate; Urea compounds such as pencycuron; Carboxylic acid amide compounds such as dimethomorph, flumorph, pyrimorph, iprovalicarb, benthiavalicarb-isopropyl, and mandipropamid; Phenyl carbamate compounds such as diethofencarb; Cyanopyrrole compounds such as fludioxonil and fenpiclonil; Strobilurin compounds such as azoxystrobin, kresoxim-methyl, metominostrobin, trifloxystrobin, picoxystrobin, orysastrobin, dimoxystrobin, pyraclostrobin, fluoxastrobin, pyraoxystrobin, pyrametostrobin, coumoxystrobin, enoxastrobin, fenaminstrobin, flufenoxystrobin, triclopyricarb, and mandestrobin; Famoxadone, oxathiapiprolin, fluorine Fluoxapiprolin Azole compounds; Thiazolecarboxamide compounds such as ethaboxam; Imidazolinone compounds such as fenamidone; Oxime ether compounds such as cyflufenamid; Anthraquinone compounds such as dithianon; Crotonic acid compounds such as meptyldinocap; Antibiotics such as validamycin, kasugamycin, streptomycin, and polyoxins; Guanidine compounds such as iminoctadine, dodine, and guazatine; Aliphatic nitrogen compounds such as 2-aminobutane (butylamine) and seboctylamine; Quinoline compounds such as tebufloquin, quinoxyfen, quinofumelin, and ipflufenoquin; Thiazolidine compounds such as flutianil; Carbamate compounds such as propamocarb hydrochloride, pyribencarb, and tolprocarb; Tetrazolyl compounds such as picarbutrazox and metyltetraprole; Allyl phenyl ketone compounds such as metrafenone and pyriofenone; Benzothiazole compounds such as probenazole and dichlobentiazox; Phenylpyrazole compounds such as fenpyrazamine; Dithiolane compounds such as isoprothiol; Picolinamide compounds such as fenpicoxamid, florylpicoxamid, and metarylpicoxamid; Pyridazine compounds such as pyridachlometyl; Sulfur compounds such as sulfur and lime sulfur; Hydrazide compounds such as chloroinconazide; Other compounds include pyroquilon, diclomezine, chloropicrin, dazomet, metam-sodium, proquinazid, spiroxamine, and dipymetitrone; Bacillus amyloliquefaciens strain QST713, Bacillus amyloliquefaciens strain FZB24, Bacillus amyloliquefaciens strain MBI600, Bacillus amyloliquefaciens strain D747, Pseudomonas fluorescens, Bacillus subtilis, and Trichoderma atroviride SKT-1; and Plant extracts such as tea tree oil.

[0045] The active ingredient compound of the insecticide, acaricide, nematicide or soil pesticide in the above-mentioned other pesticides can be appropriately selected from the following compound groups. Even if not specifically stated, when various structural isomers such as salts, alkyl esters and optical isomers exist in these compounds, these are of course also included.

[0046] Profenofos, dichlorvos, fenamiphos, fenitrothion, EPN ((RS)-(O-ethyl O-4-nitrophenyl phenylphosphonothioate), (RS)-(O-ethyl O-4-nitrophenyl phenylphosphonothioate), diazinon, chlorpyrifos, chlorpyrifos-methyl, acephate, prothiofos, disulfoton, isoxathion, isofenphos, ethion, etrimfos, quinalphos, dimethylvinphos, dimethoate, sulprofos, thiometon, vamidothion, pyraclofos, pyridaphenthion, pirimiphos-methyl, propaphos, phosalone, formothion, malathion, tetrachlorvinphos, chlorfenvinphos, Organophosphate compounds such as cyanophos, trichlorfon, methidathion, phenthoate, oxydeprofos (also known as ESP), azinphos-methyl, fenthion, heptenophos, parathion, phosphocarb, demeton-S-methyl, monocrotophos, methamidophos, parathion-methyl, terbufos, phosphamidon, phosmet, and phorate; carbaryl, propoxur, aldicarb, carbofuran, thiodicarb, methomyl, ethiofencarb, pirimicarb, fenobucarb, benfuracarb, Carbamate compounds such as bendiocarb, furathiocarb, isoprocarb, metolcarb, xylylcarb, XMC (3,5-xylyl methylcarbamate), and fenothiocarb; Nereistoxin derivatives such as cartap, thiocyclam, thiocyclam oxalate, thiocyclam hydrochloride, bensultap, thiosultap, monosultap (also known as thiosultap-monosodium), bisultap (also known as thiosultap-disodium), and polythialan; Organochlorine compounds such as dicofol, tetradifon, α-endosulfan, dienochlor, dieldrin, and methoxychlor; Organotin compounds such as fenbutatin oxide, cyhexatin, and azocyclotin; Fenvalerate, permethrin, cypermethrin, alpha-cypermethrin, zeta-cypermethrin, theta-cypermethrin, beta-cypermethrin, deltamethrin, cyhalothrin, gamma-cyhalothrin n), lambda-cyhalothrin, tefluthrin, kappa-tefluthrin, etofenprox, flufenprox, cyfluthrin, beta-cyfluthrin, fenpropathrin, flucythrinate, DL-fluvalinate, cycloprothrin in), pyrethrins, esfenvalerate, tetramethrin, resmethrin, protrifenbute, bifenthrin, kappa-bifenthrin, acrinathrin, allethrin, tau-fluvalinate, tralomethrin, proflu Pyrethroid compounds such as thrin, metofluthrin, epsilon-metofluthrin, heptafluthrin, phenothrin, flumethrin, momfluorothrin, epsilon-momfluorothrin, silafluofen, and chloroprallethrin; Benzoylurea compounds such as diflubenzuron, chlorfluazuron, teflubenzuron, flufenoxuron, lufenuron, novaluron, triflumuron, hexaflumuron, bistrifluron, noviflumuron, fluazuron, and flufenoxuron; Juvenile hormone-like compounds such as methoprene, pyriproxyfen, fenoxycarb, and diofenolan; Pyridazinone compounds such as pyridaben; Pyrazole compounds such as fenpyroximate, cyenopyrafen, and cyetpyrafen; Phenylpyrazole compounds such as acetoprole, ethiprole, fipronil, flufiprole, pyrafluprole, pyriprole, nicofluprole, and vaniliprole; Pyrazole carboxamide compounds such as pyflubumide, tebufenpyrad, tolfenpyrad, and dimpropyridaz; Pyridylpyrazole compounds such as chlorantraniliprole, cyantraniliprole, cyclaniliprole, tetrachlorantraniliprole, tetratraniliprole, tyclopyrazoflor, fluchlordiniliprole, and tiorantraniliprole; Neonicotinoid compounds such as imidacloprid, nitenpyram, acetamiprid, thiacloprid, thiamethoxam, clothianidin, dinotefuran, and nithiazine; Hydrazine compounds such as tebufenozide, methoxyfenozide, chromafenozide, and halofenozide; Pyridine compounds such as pyridalyl and flonicamid; Tetraketonate compounds such as spirodiclofen, spiromesifen, and spirobudifen; Tetramic acid compounds such as spirotetramat, spiropidion, and spidoxamat; Strobilurin compounds such as fluacrypyrim, pyriminostrobin, and flupyroxystrobin; Pyrimidine amine compounds such as flufenerim and pyrimidifen; Organosulfur compounds such as malathion; Triazine compounds such as cyromazine; Hydrazone compounds such as hydramethylnon; Phthalamide compounds such as flubendiamide, cyhalodiamide, and pioxaniliprole; Diamide compounds such as broflanilide, cyproflanilide, and piperflanilide; Thiourea compounds such as diafenthiuron and chloromethiuron; Formamidine compounds such as amitraz, chlordimeform, and chloromebuform; Pyridine azomethine compounds such as pymetrozine and pyrifluquinazon; Afoxolaner, fluralaner, fluorine Fluxametamide, sarolaner, isoflurane isocycloseram, Isoflualanam Oxazoline compounds; Fluoroalkene compounds such as trifluenfuronate; Mesoionic compounds such as dichlorothiazide, triflumezopyrim, and fenmezoditiaz; Pyropen compounds such as afidopyropen; In addition, as other compounds, buprofezin, hexythiazox, triazamate, chlorfenapyr, indoxacarb, acequinocyl, etoxazole, 1,3-dichloropropene, benclothiaz, bifenazate, propargite, clofentezine, metaflumizone, cyflumetofen, lumetofen), fenazaquin, amidoflumet, sulfluramid, hydramethylnon, metaldehyde, sulfoxaflor, verbutin, fluhexafon, tioxazafen, flometoquin, flupyradifurone, fluazaindolizine, acynonapyr, and benzylpyrimidine benzpyrimoxan, flupyrimin, Compounds such as oxazosulfyl, flupentiofenox, indazapyroxamet, sulfiflumin, and bisulflufen.

[0047] Bacillus thuringiensis aizawai, Bacillus thuringiensis kurstaki, Bacillus thuringiensis israelensis, Bacillus thuringiensis japonensis, Bacillus thuringiensis istenebrionis, Bacillus thuringiensis, Paecilomyces lilacinus, Bacillus methylotrophicus, Bacillus subtilis, Bacillus amyloliquefaciens amyloliquefaciens or Bacillus licheniformis, insect pathogenic viral agents, insect pathogenic filamentous fungi, nematode pathogenic filamentous fungi and other microbial pesticides; Antibiotics and semi-synthetic antibiotics such as emamectin benzoate, ivermectin, milbemectin, milbemycin oxime, lepimectin, spinosad, and spinetoram; Natural substances such as azadirachtin, rotenone, and ryanodine; Repellents such as DEET; Physical pest control agents such as paraffin oil and mineral oil; and RNAi pesticides such as the potato beetle RNA interference agent (ledprona).

[0048] Next, several preferred embodiments of the pest control composition of the present invention are illustrated, but these do not limit the present invention.

[0049] [1] A method for preventing and controlling clubroot disease in cruciferous vegetables in soil where Pratylenchus nematodes are present, wherein (a) a fungicide and (b) a nematicide are applied to prevent and control clubroot disease.

[0050] [2] The method according to [1], wherein the composition is applied to seeds or seedlings of cruciferous vegetables, cultivation soil, or cultivation fields, wherein the composition contains (a) a fungicide and (b) a nematicide as active ingredients.

[0051] [3] The method according to [1] or [2], wherein the fungicide (a) is at least one selected from the group consisting of fluazinam, cyazofamid, sulfaquinoxaline, and indazolesulfaquinoxaline.

[0052] [4] The method according to [1] or [2], wherein (a) the fungicide is fluazinam.

[0053] [5] The method according to [1] or [2], wherein the nematicide (b) is at least one selected from the group consisting of thiazolin, cadastrefos, cypermethrin, carbosulfan, oxamyl, fluopyram, fluthiazolin, trifluoperazine, and abamectin.

[0054] [6] The method according to [1] or [2], wherein (b) the nematicide is thiazolyl.

[0055] [7] The method according to [1] or [2], wherein the mixing ratio of (a) the fungicide to (b) the nematicide is 1:100 to 100:1 by weight.

[0056] [8] The method according to [1] or [2], wherein the mixing ratio of (a) the fungicide to (b) the nematicide is 1:10 to 5:1 by weight.

[0057] [9] The method according to [1] or [2], wherein the application method is soil spreading, soil injection, soil mixing, soil mixing in planting furrows, soil spreading in planting furrows, soil mixing in ridges and furrows, or spreading in planting furrows.

[0058]

[10] The method according to [1] or [2], wherein (a) the amount of fungicide applied is 1 g to 5,000 g per hectare, and (b) the amount of nematicide applied is 1 g to 10,000 g per hectare.

[0059]

[11] The method according to [1] or [2], wherein the cruciferous vegetable is at least one selected from turnip, Chinese cabbage, non-heading cabbage, cabbage, broccoli, cauliflower, rapeseed, rapeseed, rapeseed, white radish, Kyoto mizuna, komatsuna, takana, mizuna, mizuna, nozuna, mizuna, kale, green cabbage, radish, Brussels sprouts and wasabi.

[0060]

[12] A method for controlling clubroot of cruciferous vegetables in soil where Pratylenchus nematodes are present, wherein (a) a fungicide and (b) a nematicide are combined and applied to the cultivation soil, seeds, seedlings, or cultivation fields of the cruciferous vegetables.

[0061]

[13] A composition for controlling clubroot disease, which is used for controlling clubroot disease of cruciferous vegetables in soil where Pratylenchus nematodes are present, and contains (a) a fungicide and (b) a nematicide as active ingredients.

[0062] Example

[0063] Examples of the present invention are described below together with comparative examples, but the present invention is not limited to these examples.

[0064] Example 1 Test on the control effect of Chinese cabbage clubroot

[0065] Test drug: Fluonside powder (trade name, active ingredient: fluazinam, manufactured by Ishihara Sangyo Co., Ltd.)

[0066] Neomatrin Ace Granules (trade name, active ingredient: thiazolylphos, manufactured by Ishihara Sangyo Co., Ltd.)

[0067] Ragbi MC granules (trade name, active ingredient: cadusafos, manufactured by FMC Corporation)

[0068] Nemakiku granules (trade name, active ingredient: cyanamide, manufactured by Agrokanesho Co., Ltd.)

[0069] ビーラム granules (trade name, active ingredient: fluopyram, manufactured by バイエルクロップサイエンス Co., Ltd.)

[0070] A suspension of dormant spores of Chinese cabbage clubroot pathogen (Plasmodiophora brassicae) was added to sterilized soil adjusted to pH 6.1 with Sando Set (trade name: acidity regulator, manufactured by Sanagro Co., Ltd.) at a bacterial density of 1×10 5 The soil was inoculated at a rate of 10 ...

[0071] 59 days after treatment, the root knot index of the Chinese cabbage was measured using the following criteria, and the disease incidence and control value were calculated. Furthermore, the density of Pratylenchus penetrans in the soil was measured using the Bellman method 69 days after treatment.

[0072] Furthermore, the theoretical value (control value) based on the mixed use was calculated using the following Colby formula. The results are shown in Tables 1 and 2.

[0073] Club swelling index

[0074] 0: No root tuberculosis was confirmed

[0075] 1: Less than 25% of the root mass is attached to the root

[0076] 2: Root swelling is located on more than 25% to less than 50% of the root

[0077] 3: Root swelling is more than 50% but less than 75% of the root

[0078] 4: Root swelling is attached to more than 75% of the roots

[0079] Disease severity = [(each clubroot index × each number of plants) / total number of surveyed plants × 4] × 100

[0080] Control value = (1-a / b) × 100

[0081] a: Disease incidence in the untreated area, b: Disease incidence in the treated area

[0082] Colby formula = (X + Y) - XY / 100

[0083] X = the pest control value of component (a)

[0084] Y = the control value of component (b)

[0085] In the case where the control value is higher than the theoretical value, the composition of the present invention has a synergistic effect (unexpected effect).

[0086] [Table 1]

[0087] In the table, the numbers in ( ) are theoretical values ​​(control values)

[0088] In this experiment, the clubroot disease severity index was 40 when inoculated with Chinese cabbage clubroot bacteria alone, compared to 86 when inoculated with Pratylenchus penetrans. The presence of Pratylenchus penetrans significantly accelerated the onset of Chinese cabbage clubroot. Under these conditions, the combined use of fluazinam and each nematicide completely suppressed the onset of Chinese cabbage clubroot, significantly improving the control efficacy compared to applying each nematicide alone.

[0089] [Table 2]

[0090] When fluazinam was used in combination with thiazolin or cypermethrin, a tendency was observed to slightly reduce the soil density of Pratylenchus penetrans. However, the combined use of fluazinam and nematicides did not significantly affect the nematode density of Pratylenchus penetrans.

[0091] Example 2 Test on the control effect of Chinese cabbage clubroot

[0092] The cabbage clubroot pathogen was isolated at a density of 5 × 10 5 The soil was spray-inoculated using a spray gun (Anest Iwata Co., Ltd., Model RG-3L). Three days after inoculation, the soil was mixed using a soil mixer (Koyo Machinery Industry Co., Ltd., Model KAP-4A). Furthermore, 30 Pratylenchus penetrans were inoculated per 30g of soil and mixed manually. The next day after mixing, a solution containing the prescribed amount of the drug was sprayed using a spray gun (as above) at a rate of 10ml per 400ml of soil. After allowing the solution to air-dry, it was mixed and filled into standard pots (Japan Poly-Bag Co., Ltd., Model 75 Black Round Plastic Pot / Completely Hole-Free), and Chinese cabbage seedlings (0.5-leaf stage) were transplanted. Clubroot disease was investigated 37 days after treatment, and Pratylenchus penetrans density was investigated 8 days later.

[0093] The investigation and evaluation were carried out in the same manner as in Example 1. The results are shown in Tables 3 and 4.

[0094] Test reagent: Tributin SC (trade name, active ingredient: sulfopentamide, manufactured by Mitsui Chemicals Co., Ltd.

[0095] Gadhopre liquid (trade name, active ingredient: thiazolylphos, manufactured by Ishihara Sangyo Co., Ltd.)

[0096] Nemakiku liquid (trade name, active ingredient: cyanamide, manufactured by Agrokanesho Co., Ltd.)

[0097] Cadrusenphos standard (manufactured by Fujifilm Wako Pure Chemical Co., Ltd., dissolved in 0.05% acetone aqueous solution)

[0098] オルフィンFL (trade name, active ingredient: fluopyram, manufactured by バイエルクロップサイエンス Co., Ltd.)

[0099] Vystryl standard product (manufactured by Fujifilm Wako Pure Pharmaceutical Co., Ltd., dissolved in water for use)

[0100] Fluthiazolin standard product (produced by Hayashi Junyao Industry Co., Ltd., dissolved in dimethyl sulfoxide)

[0101] [Table 3]

[0102] In the table, the numbers in ( ) are theoretical values ​​(control values)

[0103] In the table, “-” indicates that the test was not carried out.

[0104] When sulfamethoxam was mixed with each nematicide, the occurrence of Chinese cabbage clubroot was suppressed in all combinations, and the control effect was significantly improved compared with the application of each nematicide alone.

[0105] [Table 4]

[0106] In the table, “-” indicates that the test was not carried out.

[0107] When sulfamethoxam was used in combination with thiabendazole, cadusafos, fluopyram, oxamyl, or fluthiazolinone, a slight decrease in the soil density of Pratylenchus penetrans was sometimes observed. However, the combination of sulfamethoxam and nematicides did not significantly affect the nematode density of Pratylenchus penetrans.

[0108] Example 3 Test on the control effect of Chinese cabbage clubroot

[0109] The cabbage clubroot pathogen was isolated at a density of 5 × 10 5 The soil was sprayed with a spray gun (Anest Iwata Co., Ltd., Model RG-3L) and mixed the next day using a soil mixer (Koyo Machinery Industry Co., Ltd., Model KAP-4A). Furthermore, 15 Pratylenchus penetrans were inoculated per 30g of soil and mixed manually. The next day after mixing, a solution containing the prescribed amount of the drug was sprayed using a spray gun (as above) at a rate of 10ml per 400ml of soil. After allowing the solution to air-dry, it was mixed and filled into standard pots (Japan Polyboro Trading Co., Ltd., Model 75 Black Round Plastic Pot / Completely Hole-Free), and Chinese cabbage seedlings (0.5-leaf stage) were transplanted. Clubroot disease was investigated 34 days after treatment, and Pratylenchus penetrans density was investigated 9 days later.

[0110] The investigation and evaluation were carried out in the same manner as in Example 1. The results are shown in Tables 5 and 6.

[0111] Test drug: Fluonside SC (trade name, active ingredient: fluazinam, manufactured by Ishihara Sangyo Co., Ltd.)

[0112] Gadhopre liquid (trade name, active ingredient: thiazolylphos, manufactured by Ishihara Sangyo Co., Ltd.)

[0113] Nemakiku liquid (trade name, active ingredient: cyanamide, manufactured by Agrokanesho Co., Ltd.)

[0114] Cadrusenphos standard (manufactured by Fujifilm Wako Pure Chemical Co., Ltd., dissolved in 0.05% acetone aqueous solution)

[0115] オルフィンFL (trade name, active ingredient: fluopyram, manufactured by バイエルクロップサイエンス Co., Ltd.)

[0116] Vystryl standard product (manufactured by Fujifilm Wako Pure Pharmaceutical Co., Ltd., dissolved in water for use)

[0117] Fluthiazolin standard product (produced by Hayashi Junyao Industry Co., Ltd., dissolved in dimethyl sulfoxide)

[0118] [Table 5]

[0119] In the table, the numbers in ( ) are theoretical values ​​(control values)

[0120] In the table, “-” indicates that the test was not carried out.

[0121] When fluazinam and each nematicide were used in combination at varying application rates, the incidence of Chinese cabbage clubroot was almost completely suppressed in all combinations, and the control effect was significantly improved compared to when each nematicide was used alone.

[0122] [Table 6]

[0123] In the table, “-” indicates that the test was not carried out.

[0124] When fluazinam was used in combination with thiazolinone, cadusafos, fluopyram, oxamyl, or fluthiazolinone, a tendency was sometimes observed to slightly decrease the soil density of Pratylenchus penetrans. However, the combined use of fluazinam and nematicides did not significantly affect the nematode density of Pratylenchus penetrans.

[0125] Example 4: Test on the Control Effect of Chinese Cabbage Clubroot

[0126] The cabbage clubroot pathogen was isolated at a density of 5 × 10 5The soil was sprayed with a spray gun (Anest Iwata Co., Ltd., Model RG-3L) and mixed four days later using a soil mixer (Koyo Machinery Industry Co., Ltd., Model KAP-4A). Furthermore, 10 Pratylenchus penetrans were inoculated per 30g of soil and mixed manually. The day after mixing, a solution containing the prescribed amount of the drug was sprayed using a spray gun (as above) at a rate of 10ml per 400ml of soil. After allowing the solution to air dry, it was mixed and filled into standard pots (Japan Polyboro Trading Co., Ltd., Model 75 Black Round Plastic Pot / Completely Hole-Free), and Chinese cabbage seedlings (0.5-leaf stage) were transplanted. Clubroot disease was investigated 49 days after treatment, and Pratylenchus penetrans density was investigated two days later.

[0127] The investigation and evaluation were carried out in the same manner as in Example 1, and the results are shown in Tables 7 to 12.

[0128] Test agent: Olacure water-dispersible granules (trade name, active ingredient: indazole sulfamethoxazole, manufactured by Nissan Chemical Co., Ltd.)

[0129] Fluonside SC (trade name, active ingredient: fluazinam, manufactured by Ishihara Sangyo Co., Ltd.)

[0130] Sulfonate SC (trade name, active ingredient: sulfopentamide, manufactured by Mitsui Chemicals Co., Ltd.

[0131] Gadhopre liquid (trade name, active ingredient: thiazolylphos, manufactured by Ishihara Sangyo Co., Ltd.)

[0132] Nemakiku liquid (trade name, active ingredient: cyanamide, manufactured by Agrokanesho Co., Ltd.)

[0133] Cadrusenphos standard (manufactured by Fujifilm Wako Pure Chemical Co., Ltd., dissolved in 0.05% acetone aqueous solution)

[0134] オルフィンFL (trade name, active ingredient: fluopyram, manufactured by バイエルクロップサイエンス Co., Ltd.)

[0135] Vystryl standard product (manufactured by Fujifilm Wako Pure Pharmaceutical Co., Ltd., dissolved in water for use)

[0136] Fluthiazolin standard product (produced by Hayashi Junyao Industry Co., Ltd., dissolved in dimethyl sulfoxide)

[0137] [Table 7]

[0138] In the table, the numbers in ( ) are theoretical values ​​(control values)

[0139] In the table, “-” indicates that the test was not carried out.

[0140] When indazolamide and each nematicide were used in combination at varying application rates, the onset of Chinese cabbage clubroot was suppressed in all combinations, and the control effect was significantly improved compared to when each nematicide was used alone.

[0141] [Table 8]

[0142] In the table, “-” indicates that the test was not carried out.

[0143] When indazolamide was used in combination with fluopyram, oxamyl, or fluthiazolinone, a tendency was sometimes observed to slightly decrease the soil density of Pratylenchus penetrans. However, the combined use of indazolamide and nematicides did not significantly affect the nematode density of Pratylenchus penetrans.

[0144] [Table 9]

[0145] In the table, the numbers in ( ) are theoretical values ​​(control values)

[0146] When fluazinam was mixed with each nematicide, the occurrence of Chinese cabbage clubroot was suppressed in all combinations, and the control effect was significantly improved compared with the application of each nematicide alone.

[0147] [Table 10]

[0148] When fluazinam was used in combination with cypermethrin, cadusafos, fluopyram, or oxamyl, a tendency was observed to slightly reduce the soil density of Pratylenchus penetrans. However, the combination of fluazinam and nematicides did not significantly affect the nematode density of Pratylenchus penetrans.

[0149] [Table 11]

[0150] In the table, the numbers in ( ) are theoretical values ​​(control values)

[0151] When sulfamethoxam was mixed with each nematicide, the occurrence of Chinese cabbage clubroot was suppressed in all combinations, and the control effect was significantly improved compared with the application of each nematicide alone.

[0152] [Table 12]

[0153] When sulfamethoxam was used in combination with cadusofos or oxamyl, a tendency was observed to slightly reduce the density of Pratylenchus penetrans in the soil. However, the combined use of sulfamethoxam and nematicides did not significantly affect the density of Pratylenchus penetrans.

[0154] Example 5 Chinese cabbage clubroot disease prevention and control effect test

[0155] The cabbage clubroot pathogen was isolated at a density of 5 × 10 5 The soil was sprayed and inoculated using a spray gun (Anest Iwata Co., Ltd., Model RG-3L). The next day, the soil was mixed using a soil mixer (Koyo Machinery Co., Ltd., Model KAP-4A). Furthermore, 10 Pratylenchus penetrans were inoculated per 30g of soil and mixed manually. The next day, a solution containing a prescribed amount of the drug was sprayed using a spray gun (as above) at a rate of 10ml per 400ml of soil. After allowing the solution to air dry, it was mixed and filled into standard pots (Japan Polyboro Trading Co., Ltd., Model 75 Black Round Plastic Pot / Completely Hole-Free), and Chinese cabbage seedlings (0.5-leaf stage) were transplanted. Clubroot disease was investigated 48 days after treatment, and Pratylenchus penetrans density was investigated 6 days later.

[0156] The investigation and evaluation were carried out in the same manner as in Example 1. The results are shown in Tables 13 and 14.

[0157] Test agent: Lanman FL (trade name, active ingredient: cyazofamid, manufactured by Ishihara Sangyo Co., Ltd.)

[0158] Gadhopre liquid (trade name, active ingredient: thiazolylphos, manufactured by Ishihara Sangyo Co., Ltd.)

[0159] Nemakiku liquid (trade name, active ingredient: cyanamide, manufactured by Agrokanesho Co., Ltd.)

[0160] Cadrusenphos standard (manufactured by Fujifilm Wako Pure Chemical Co., Ltd., dissolved in 0.05% acetone aqueous solution)

[0161] オルフィンFL (trade name, active ingredient: fluopyram, manufactured by バイエルクロップサイエンス Co., Ltd.)

[0162] Vystryl standard product (manufactured by Fujifilm Wako Pure Pharmaceutical Co., Ltd., dissolved in water for use)

[0163] Fluthiazolin standard product (produced by Hayashi Junyao Industry Co., Ltd., dissolved in dimethyl sulfoxide)

[0164] [Table 13]

[0165] In the table, the numbers in ( ) are theoretical values ​​(control values)

[0166] In the table, “-” indicates that the test was not carried out.

[0167] When cyazofamid and each nematicide were mixed and applied at varying application rates, the incidence of clubroot disease in Chinese cabbage was almost completely suppressed in all combinations, and the control effect was significantly improved compared to applying each nematicide alone.

[0168] [Table 14]

[0169] In the table, “-” indicates that the test was not carried out.

[0170] When cyazofamid was used in combination with cadusafos or fluthiazolin, a tendency was sometimes observed for a slight decrease in the soil density of Pratylenchus penetrans. However, the combined use of cyazofamid with nematicides did not significantly affect the nematode density of Pratylenchus penetrans.

[0171] Industrial availability

[0172] The present invention is useful as a method for controlling clubroot in soil where clubroot frequently occurs due to the presence of Pratylenchus nematodes, and has industrial applicability.

[0173] In addition, the entire contents of the specification, claims, and abstract of Japanese Patent Application No. 2023-036011 filed on March 8, 2023 are incorporated herein by reference in their entirety and are adopted as the disclosed contents of the specification of the present invention.

Claims

1. A method for preventing and controlling clubroot disease in cruciferous vegetables in soil where Pratylenchus nematodes are present, wherein: Apply (a) fungicides and (b) nematicides to control clubroot.

2. The method according to claim 1, wherein the composition is applied to seeds or seedlings of cruciferous vegetables, cultivation soil, or cultivation fields, wherein the composition is characterized in that it contains (a) a fungicide and (b) a nematicide as active ingredients.

3. The method according to claim 1 or 2, wherein (a) the fungicide is at least one selected from the group consisting of fluazinam, cyazofamid, sulfaquinoxaline and indazolesulfaquinoxaline.

4. The method according to claim 1 or 2, wherein the nematicide (b) is at least one selected from the group consisting of thiazolin, cadasphos, cypermethrin, carbosulfan, oxamyl, fluopyram, fluthiazolin, trifluoperazine, and abamectin.

5. The method according to claim 1 or 2, wherein the mixing ratio of (a) the fungicide to (b) the nematicide is 1:100 to 100:1 by weight.

6. The method according to claim 1 or 2, wherein the application method is soil spreading, soil irrigation, soil mixing, planting furrow soil mixing, soil spreading in planting furrows, ridge furrow soil mixing or planting furrow spreading.

7. The method according to claim 1 or 2, wherein the application amount of (a) the fungicide is 1 g to 5,000 g per hectare, and the application amount of (b) the nematicide is 1 g to 10,000 g per hectare.

8. The method according to claim 1 or 2, wherein the cruciferous vegetable is at least one selected from the group consisting of turnip, Chinese cabbage, non-heading cabbage, cabbage, broccoli, cauliflower, rapeseed, rapeseed, white radish, Kyoto mizuna, komatsuna, takana, mizuna, mizuna, nori, mizuna, kale, green cabbage, cherry radish, Brussels sprouts and wasabi.

9. A method for controlling clubroot of cruciferous vegetables in soil in which Pratylenchus nematodes are present, wherein: (a) A fungicide and (b) a nematicide are combined and applied to the cultivation soil, seeds, seedlings, and cultivation fields of cruciferous vegetables.

10. A clubroot control composition for controlling clubroot of cruciferous vegetables in soil where Pratylenchus nematodes are present, the composition comprising (a) a fungicide and (b) a nematicide as active ingredients.

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