A bacteriostatic antioxidant composition and its use in an anti-hair loss shampoo

Abstract

The application discloses an antibacterial and antioxidant composition and application thereof in anti-hair loss shampoo, and the antibacterial and antioxidant composition is prepared by a method comprising the following steps: crushing and sieving folium artemisiae argyi, agastache, peach tree branches, willow branches, sophora japonica branches and cypress leaves respectively, mixing folium artemisiae argyi powder, agastache powder, peach tree branch powder, willow branch powder, sophora japonica branch powder and cypress leaf powder, adding a solvent for soaking, heating and refluxing for extraction, filtering while hot, centrifuging the filtrate, concentrating the supernatant, and drying to obtain the antibacterial and antioxidant composition; the folium artemisiae argyi, agastache, peach tree branches, willow branches, sophora japonica branches and cypress leaves are compounded and extracted, the obtained composition can simultaneously play the roles of antioxidation and bacteriostasis, and further play the effects of anti-hair loss and inhibition of scalp itching.

Description

Technical Field

[0001] This invention belongs to the field of shampoo technology, and particularly relates to an antibacterial and antioxidant composition and its application in anti-hair loss shampoo. Background Technology

[0002] Most shampoos on the market currently use sulfate-based surfactants and silicone oil as a hair conditioner. Although sulfates and silicone oil can improve the cleaning effect and user experience of shampoos, these ingredients can easily damage the hair cuticle, leading to hair damage, increasing the probability of hair loss, and causing negative problems such as excessive hair loss.

[0003] Besides using shampoos that damage hair, oxidative stress is one of the key mechanisms causing hair graying and loss, and is also one of the reasons for early-onset androgenetic alopecia in young patients. Many patients, in addition to hair loss, also experience symptoms such as dandruff, itchy scalp, and small red bumps, which are related to bacterial or fungal infections of the scalp.

[0004] To address hair loss, most hair loss prevention products on the market temporarily inhibit DHT (a hair loss trigger) or dilate blood vessels by adding ingredients such as caffeine and ketoconazole. While these methods can achieve good short-term results, long-term use is inconsistent, and rebound effects are possible after discontinuation. Therefore, natural herbal hair loss prevention products with mild effects, few side effects, and potential long-term conditioning benefits have a huge market potential. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides an antibacterial and antioxidant composition. This invention combines and extracts Artemisia argyi, patchouli, peach tree branches, willow tree branches, Sophora japonica tree branches, and Platycladus orientalis leaves. The resulting composition can simultaneously exert antioxidant and antibacterial effects, thereby preventing hair loss and inhibiting scalp itching.

[0006] The purpose of this invention is to provide an antibacterial and antioxidant composition, which is prepared by a method comprising the following steps:

[0007] Artemisia argyi, patchouli, peach tree branches, willow tree branches, locust tree branches, and arborvitae leaves were crushed and sieved. The powders of Artemisia argyi, patchouli, peach tree branches, willow tree branches, locust tree branches, and arborvitae leaves were mixed, soaked in solvent, heated and refluxed for extraction, filtered while hot, centrifuged the filtrate, concentrated the supernatant, and dried to obtain an antibacterial and antioxidant composition.

[0008] In some embodiments of the present invention, the mass ratio of the mugwort powder, patchouli powder, peach twig powder, willow twig powder, sophora japonica twig powder, and arborvitae leaf powder is 0.8~1.2:0.8~1.2:1.2~1.8:1.2~1.8:1.2~1.8:1.7~2.3.

[0009] In some embodiments of the present invention, the solvent is selected from water or a 10-50 wt% aqueous ethanol solution.

[0010] In some embodiments of the present invention, the solvent is selected from 40-50 wt% aqueous ethanol solution.

[0011] In some embodiments of the present invention, the total mass ratio of the artemisia leaf powder, patchouli powder, peach branch powder, willow branch powder, sophora branch powder, and arborvitae leaf powder to the solvent is 1:10~20.

[0012] In some embodiments of the present invention, the soaking time is 1 to 2 hours.

[0013] In some embodiments of the present invention, the heating and reflux extraction time is 1.5 to 3 hours.

[0014] In some embodiments of the present invention, the sieving is performed through a 20-40 mesh sieve.

[0015] Another object of the present invention is to provide an anti-hair loss shampoo, comprising 0.1-0.7 wt% of the antibacterial and antioxidant composition and 99.3-99.9 wt% of excipients.

[0016] Another object of the present invention is to provide a method for preparing the aforementioned anti-hair loss shampoo, comprising the following steps:

[0017] The aqueous solution of the antibacterial and antioxidant composition and the excipients are mixed and stirred evenly to obtain an anti-hair loss shampoo.

[0018] In some embodiments of the present invention, the mass concentration of the aqueous solution of the antibacterial and antioxidant composition is 1~7 mg / mL.

[0019] In some embodiments of the present invention, the excipients include 50-60 wt% water, 15-25 wt% sodium α-olefin sulfonate, 10-20 wt% cocamidopropyl betaine, 2-4 wt% cocoyl glucoside, 0.2-0.4 wt% methyl glucoside dioleate, 0.5-1.5 wt% biomimetic phospholipids, 0.2-0.4 wt% cocoyl methyl monoethanolamide, 0.3-0.7 wt% D-panthenol, 0.5-1.5 wt% glycerol, 0.05-0.2 wt% dipotassium glycyrrhizate, 0.2-0.4 wt% polyquaternium-10, 0.05-0.2 wt% disodium ethylenediaminetetraacetate, 0.1-0.3 wt% piroctone ketone ethanolamine, 1-3 wt% acrylate copolymers, 0.4-0.6 wt% phenoxyethanol, 0.3-0.7 wt% sodium benzoate, and polyquaternium-7. 0.2-0.4 wt%, fragrance 0.1-1 wt%, and citric acid 0.1-1 wt%.

[0020] In some embodiments of the present invention, the mixing temperature is not higher than 45°C.

[0021] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0022] (1) The mugwort used in this invention has the effects of removing dampness and relieving itching; patchouli has the effects of aromatic turbidity removal, dampness removal and spleen invigoration, blood nourishment and hair growth, liver soothing and depression relief, heat clearing and detoxification; peach tree branches have the effects of blood circulation and collaterals, detoxification and insecticidal effects; willow tree branches have the effects of wind removal and dampness removal, detoxification and swelling reduction; locust tree branches have the effects of hemostasis, wind removal and dampness drying, and can be used externally to treat skin itching and scabies; arborvitae leaves have the effects of cooling blood and stopping bleeding, resolving phlegm and relieving cough, hair growth and blackening, and can be used to treat blood heat hair loss and premature graying of hair; through the reasonable combination of the above six Chinese medicines, the effects of clearing heat and drying dampness, wind removal and relieving itching are achieved.

[0023] (2) The present invention combines Artemisia argyi, patchouli, peach tree branches, willow tree branches, Sophora japonica tree branches and Platycladus orientalis leaves, and extracts them. The resulting composition can simultaneously scavenge DPPH free radicals, inhibit Staphylococcus aureus and Escherichia coli, thereby preventing hair loss and inhibiting scalp itching. Attached Figure Description

[0024] Figure 1 The inhibitory effect of the antibacterial and antioxidant composition of Example 1 on Staphylococcus aureus is shown.

[0025] Figure 2 The inhibitory effect of the antibacterial and antioxidant composition of Example 2 on Staphylococcus aureus is shown.

[0026] Figure 3 The inhibitory effect of the antibacterial and antioxidant composition of Example 3 on Staphylococcus aureus is shown.

[0027] Figure 4 The inhibitory effect of the antibacterial and antioxidant composition of Example 4 on Staphylococcus aureus is shown.

[0028] Figure 5 The inhibitory effect of the antibacterial and antioxidant composition of Example 5 on Staphylococcus aureus is shown.

[0029] Figure 6 The inhibitory effect of the antibacterial and antioxidant composition of Example 6 on Staphylococcus aureus is shown.

[0030] Figure 7 The inhibitory effect of the antibacterial and antioxidant composition of Example 1 on Escherichia coli is shown.

[0031] Figure 8 The inhibitory effect of the antibacterial and antioxidant composition of Example 2 on Escherichia coli is shown.

[0032] Figure 9 The inhibitory effect of the antibacterial and antioxidant composition of Example 3 on Escherichia coli is shown.

[0033] Figure 10 The inhibitory effect of the antibacterial and antioxidant composition of Example 4 on Escherichia coli is shown.

[0034] Figure 11 The inhibitory effect of the antibacterial and antioxidant composition of Example 5 on Escherichia coli is shown.

[0035] Figure 12 The inhibitory effect of the antibacterial and antioxidant composition of Example 6 on Escherichia coli is shown.

[0036] Figure 13 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Example 1.

[0037] Figure 14 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Example 2.

[0038] Figure 15 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Example 3.

[0039] Figure 16 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Example 4.

[0040] Figure 17 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Example 5.

[0041] Figure 18 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Example 6.

[0042] Figure 19 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 1 on Staphylococcus aureus is shown.

[0043] Figure 20 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 2 on Staphylococcus aureus was measured.

[0044] Figure 21 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 3 on Staphylococcus aureus was measured.

[0045] Figure 22 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 4 on Staphylococcus aureus was measured.

[0046] Figure 23 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 5 on Staphylococcus aureus was measured.

[0047] Figure 24The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 6 on Staphylococcus aureus was measured.

[0048] Figure 25 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 7 on Staphylococcus aureus is shown.

[0049] Figure 26 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 8 on Staphylococcus aureus was measured.

[0050] Figure 27 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 1 on Escherichia coli is shown.

[0051] Figure 28 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 2 on Escherichia coli was measured.

[0052] Figure 29 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 3 on Escherichia coli was measured.

[0053] Figure 30 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 4 on Escherichia coli was measured.

[0054] Figure 31 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 5 on Escherichia coli was measured.

[0055] Figure 32 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 6 on Escherichia coli was measured.

[0056] Figure 33 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 7 on Escherichia coli was measured.

[0057] Figure 34 The inhibitory effect of the antibacterial and antioxidant composition of Comparative Example 8 on Escherichia coli was measured.

[0058] Figure 35 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Comparative Example 1.

[0059] Figure 36 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Comparative Example 2.

[0060] Figure 37 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Comparative Example 3.

[0061] Figure 38 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition in Comparative Example 4.

[0062] Figure 39 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Comparative Example 5.

[0063] Figure 40 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition in Comparative Example 6.

[0064] Figure 41 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Comparative Example 7.

[0065] Figure 42 The value represents the DPPH free radical scavenging rate of the antibacterial and antioxidant composition of Comparative Example 8. Detailed Implementation

[0066] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions in the embodiments of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this invention.

[0067] Example 1

[0068] This embodiment provides an antibacterial and antioxidant composition, the preparation method of which specifically includes the following steps:

[0069] Artemisia argyi, patchouli, peach tree branches, willow tree branches, sophora japonica tree branches, and arborvitae leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 10g of patchouli powder, 15g of peach tree branch powder, 15g of willow tree branch powder, 15g of sophora japonica tree branch powder, and 20g of arborvitae leaf powder were mixed together and soaked in 850g of water for 0.5-1h. The mixture was heated and refluxed for 1.5h and then filtered while hot. The residue was soaked again in 850g of water for 0.5-1h and heated and refluxed for 1.5h and then filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0070] Example 2

[0071] This embodiment provides an antibacterial and antioxidant composition, which differs from Example 1 only in that the extraction solvent is replaced with a 10wt% ethanol aqueous solution. The preparation method specifically includes the following steps:

[0072] Artemisia argyi, patchouli, peach tree branches, willow tree branches, sophora japonica tree branches, and arborvitae leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 10g of patchouli powder, 15g of peach tree branch powder, 15g of willow tree branch powder, 15g of sophora japonica tree branch powder, and 20g of arborvitae leaf powder were mixed together and soaked in 850g of 10wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and then filtered while hot. The residue was soaked again in 850g of 10wt% ethanol aqueous solution for 0.5-1h and then heated and refluxed for 1.5h and then filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0073] Example 3

[0074] This embodiment provides an antibacterial and antioxidant composition, which differs from Example 1 only in that the extraction solvent is replaced with a 20wt% ethanol aqueous solution. The preparation method specifically includes the following steps:

[0075] Artemisia argyi, patchouli, peach tree branches, willow tree branches, sophora japonica tree branches, and arborvitae leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 10g of patchouli powder, 15g of peach tree branch powder, 15g of willow tree branch powder, 15g of sophora japonica tree branch powder, and 20g of arborvitae leaf powder were mixed together and soaked in 850g of 20wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and then filtered while hot. The residue was soaked again in 850g of 20wt% ethanol aqueous solution for 0.5-1h and then heated and refluxed for 1.5h and then filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0076] Example 4

[0077] This embodiment provides an antibacterial and antioxidant composition, which differs from Example 1 only in that the extraction solvent is replaced with a 30wt% ethanol aqueous solution instead of water. The preparation method specifically includes the following steps:

[0078] Artemisia argyi, patchouli, peach tree branches, willow tree branches, sophora japonica tree branches, and arborvitae leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 10g of patchouli powder, 15g of peach tree branch powder, 15g of willow tree branch powder, 15g of sophora japonica tree branch powder, and 20g of arborvitae leaf powder were mixed together and soaked in 850g of 30wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and then filtered while hot. The residue was soaked again in 850g of 30wt% ethanol aqueous solution for 0.5-1h and then heated and refluxed for 1.5h and then filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0079] Example 5

[0080] This embodiment provides an antibacterial and antioxidant composition, which differs from Example 1 only in that the extraction solvent is replaced with a 40wt% ethanol aqueous solution. The preparation method specifically includes the following steps:

[0081] Artemisia argyi, patchouli, peach tree branches, willow tree branches, sophora japonica tree branches, and arborvitae leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 10g of patchouli powder, 15g of peach tree branch powder, 15g of willow tree branch powder, 15g of sophora japonica tree branch powder, and 20g of arborvitae leaf powder were mixed together and soaked in 850g of 40wt% ethanol aqueous solution for 0.5-1h. The mixture was then heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 40wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0082] Example 6

[0083] This embodiment provides an antibacterial and antioxidant composition, which differs from Example 1 only in that the extraction solvent is replaced with a 50wt% ethanol aqueous solution instead of water. The preparation method specifically includes the following steps:

[0084] Artemisia argyi, patchouli, peach tree branches, willow tree branches, sophora japonica tree branches, and arborvitae leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 10g of patchouli powder, 15g of peach tree branch powder, 15g of willow tree branch powder, 15g of sophora japonica tree branch powder, and 20g of arborvitae leaf powder were mixed together and soaked in 850g of 50wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and then filtered while hot. The residue was soaked again in 850g of 50wt% ethanol aqueous solution for 0.5-1h and then heated and refluxed for 1.5h and then filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0085] Examples 7-12

[0086] The antibacterial and antioxidant compositions of Examples 7-12 were obtained by replacing "10g of Artemisia argyi powder, 10g of Patchouli powder, 15g of Peach twig powder, 15g of Willow twig powder, 15g of Sophora japonica twig powder, and 20g of Platycladus orientalis leaf powder" in Examples 1-6 with "8g of Artemisia argyi powder, 12g of Patchouli powder, 12g of Peach twig powder, 18g of Willow twig powder, 12g of Sophora japonica twig powder, and 23g of Platycladus orientalis leaf powder".

[0087] Examples 13-18

[0088] The antibacterial and antioxidant compositions of Examples 13-18 were obtained by replacing "10g of Artemisia argyi powder, 10g of Patchouli powder, 15g of Peach twig powder, 15g of Willow twig powder, 15g of Sophora japonica twig powder, and 20g of Platycladus orientalis leaf powder" in Examples 1-6 with "12g of Artemisia argyi powder, 8g of Patchouli powder, 18g of Peach twig powder, 12g of Willow twig powder, 18g of Sophora japonica twig powder, and 17g of Platycladus orientalis leaf powder".

[0089] Example 19

[0090] This embodiment provides an anti-hair loss shampoo, the preparation method of which specifically includes the following steps:

[0091] S1. Based on 100% of the shampoo formula base, by mass percentage, the following are the ingredients: deionized water (balance), sodium α-olefin sulfonate (20%), cocamidopropyl betaine (15%), cocoyl glucoside (3%), methyl glucoside (0.3%), biomimetic phospholipids (1%), methyl monoethanolamide cocoate (0.3%), D-panthenol (0.5%), glycerol (1%), dipotassium glycyrrhizate (0.1%), polyquaternium-10 (0.3%), and disodium EDTA (0.1%). The mixture is heated to 80-85°C and stirred until dissolved. The temperature is lowered to 50°C, and 0.2% piroctone ketone ethanolamine salt is added and stirred until completely dissolved. Then, 2% acrylate copolymer is added. The temperature is lowered to 45°C, and 0.5% phenoxyethanol, 0.5% sodium benzoate, and 0.3% polyquaternium-7 are added. Finally, 0.2% fragrance and 0.25% citric acid are added to obtain the shampoo formula base.

[0092] S2. Mix an aqueous solution of the antibacterial and antioxidant composition of Example 1 with a mass concentration of 4 mg / mL (wherein the mass of the antibacterial and antioxidant composition of Example 1 is 0.4 g) with 99.6 g of shampoo formula base, and mix at a temperature not exceeding 45°C until homogeneous to obtain an anti-hair loss shampoo.

[0093] Examples 20-36

[0094] By replacing "aqueous solution of antibacterial and antioxidant composition of Example 1" in Example 19 with "aqueous solution of antibacterial and antioxidant composition of Examples 2-18", anti-hair loss shampoos of Examples 20-36 were obtained respectively.

[0095] Performance testing:

[0096] (1) Antibacterial performance test:

[0097] Operating steps:

[0098] ① Bacterial culture: A single colony was picked and inoculated into nutrient broth medium, and cultured at 37 ℃ and 200 rpm for 6 h with shaking. The bacterial concentration of the culture after 6 h of growth was determined by the dilution agar plate counting method to be 10.8 ~10 9 CFU / mL. The bacterial culture grown over 6 hours was then diluted to ~10⁻¹⁰ using nutrient broth. 6 CFU / mL, for later use.

[0099] ② Preparation of diluted compositions and inoculation with bacterial culture: The antibacterial and antioxidant compositions of Examples 1-6 were diluted twice to different concentrations using sterile LB medium. The concentrations of the antibacterial and antioxidant compositions of Examples 1-6 after dilution were, in order: 128 mg / mL, 64 mg / mL, 32 mg / mL, 16 mg / mL, 8 mg / mL, 4 mg / mL, 2 mg / mL, 1 mg / mL, 0.5 mg / mL, 0.25 mg / mL, and 0.125 mg / mL.

[0100] ③ Bacterial inoculation: Add 100 μL of the prepared bacterial culture to a 96-well cell culture plate, and mix with 100 μL of the diluted antibacterial and antioxidant compositions from Examples 1-6. The positive control group is the culture medium inoculated with bacteria. Each group has 3 parallel wells.

[0101] ④Incubation: Incubate the 96-well cell culture plate in a 37 °C constant temperature incubator for 16 h to 20 h.

[0102] ⑤ Staining: After incubation, add 20 μL of 1% red tetrazolium solution and incubate for another 30 min, then observe the color change.

[0103] ⑥ Result Interpretation: A red color indicates bacterial growth, and the minimum concentration corresponding to the well that does not turn red is the sample MIC. The results are shown in Table 1.

[0104] Table 1. Antibacterial and antioxidant properties of the antibacterial and antioxidant compositions in Examples 1-6.

[0105] sample Staphylococcus aureus (MIC) Escherichia coli (MIC) Example 1 4mg / mL 64mg / mL Example 2 4mg / mL 32mg / mL Example 3 4mg / mL 16mg / mL Example 4 500 μg / mL 8mg / mL Example 5 250 μg / mL 4mg / mL Example 6 250 μg / mL 4mg / mL

[0106] As shown in Table 1, the antibacterial and antioxidant compositions of Examples 1-6 of the present invention exhibit good antibacterial properties against Staphylococcus aureus and Escherichia coli. The antibacterial and antioxidant compositions obtained by extracting the raw materials with a 40-50 wt% ethanol aqueous solution show even better antibacterial properties against both Staphylococcus aureus and Escherichia coli. Meanwhile, the antibacterial and antioxidant compositions of Examples 7-12 and Examples 13-18 show similar antibacterial effects against Staphylococcus aureus and Escherichia coli to those of Examples 1-6, meaning that the antibacterial and antioxidant compositions obtained by extracting the raw materials with a 40-50 wt% ethanol aqueous solution also exhibit even better antibacterial properties against both Staphylococcus aureus and Escherichia coli.

[0107] (2) Antioxidant performance determination:

[0108] Operating steps:

[0109] ① Vitamin C was used as a positive control in all antioxidant capacity tests.

[0110] ② Take multiple test tubes and precisely add 3 mL of ethanol (60 wt%) aqueous solution of the antibacterial and antioxidant composition of Examples 1-6, then add 1 mL of 60 wt% ethanol aqueous solution of DPPH with a concentration of 0.2 mmol / L, shake well, and place in the dark at room temperature for 30 min. Measure the absorbance at 517 nm and record the result as Ai.

[0111] ③ Drug blank control: Take 3 mL of ethanol (60 wt%) aqueous solution of the antibacterial and antioxidant composition of Examples 1-6, add 1 mL of 60 wt% ethanol aqueous solution, place in the dark at room temperature for 30 min, and measure the absorbance at 517 nm. The result is recorded as Aj.

[0112] ④The absorbance of the DPPH reference standard was measured under the same conditions, and the result was recorded as A0.

[0113] ⑤ Calculate the DPPH scavenging rate of each sample concentration using the following scavenging rate formula, and calculate the half-maximal inhibitory concentration (ICP) of the DPPH free radical scavenging activity of each sample;

[0114] Clearance rate = ;

[0115] ⑥ The DPPH free radical scavenging activity of the antibacterial and antioxidant compositions of Examples 1-6 was determined according to the above steps. The results are shown in Table 2.

[0116] Table 2. Half-maximal inhibitory concentrations of DPPH free radical scavenging activity of the antibacterial and antioxidant compositions of Examples 1-6.

[0117] sample IC50 (mg / mL) Example 1 0.0230 Example 2 0.0151 Example 3 0.0221 Example 4 0.0172 Example 5 0.0162 Example 6 0.0157

[0118] As shown in Tables 1 and 2, the antibacterial and antioxidant compositions of Examples 1-6 of the present invention exhibit good DPPH free radical scavenging ability. Specifically, the antibacterial and antioxidant compositions obtained by extracting the raw materials with a 40-50 wt% ethanol aqueous solution not only demonstrate better antibacterial performance against Staphylococcus aureus and Escherichia coli, but also possess good DPPH free radical scavenging ability. Furthermore, the antibacterial and antioxidant compositions of Examples 7-12 and Examples 13-18 show similar DPPH free radical scavenging abilities to those of Examples 1-6, i.e., the antibacterial and antioxidant compositions obtained by extracting the raw materials with a 40-50 wt% ethanol aqueous solution not only demonstrate better antibacterial performance against Staphylococcus aureus and Escherichia coli, but also possess good DPPH free radical scavenging ability.

[0119] Comparative Example 1

[0120] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 5 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of patchouli powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0121] Artemisia argyi, patchouli, and Platycladus orientalis leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of patchouli powder, and 20g of Platycladus orientalis leaf powder were mixed and soaked in 850g of 40wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 40wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0122] Comparative Example 2

[0123] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 5 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of willow twig powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0124] Artemisia argyi, willow branches, and arborvitae leaves were crushed separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of willow branch powder, and 20g of arborvitae leaf powder were mixed and soaked in 850g of 40wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 40wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0125] Comparative Example 3

[0126] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 5 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of sophora japonica twig powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0127] Artemisia argyi, Sophora japonica branches, and Platycladus orientalis leaves were crushed separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of Sophora japonica branch powder, and 20g of Platycladus orientalis leaf powder were mixed and soaked in 850g of 40wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 40wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0128] Comparative Example 4

[0129] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 5 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of peach twig powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0130] Artemisia argyi, peach tree branches, and Platycladus orientalis leaves were crushed separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of peach tree branch powder, and 20g of Platycladus orientalis leaf powder were mixed and soaked in 850g of 40wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 40wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0131] Comparative Example 5

[0132] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 6 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of patchouli powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0133] Artemisia argyi, patchouli, and Platycladus orientalis leaves were pulverized separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of patchouli powder, and 20g of Platycladus orientalis leaf powder were mixed and soaked in 850g of 50wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 50wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0134] Comparative Example 6

[0135] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 6 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of willow twig powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0136] Artemisia argyi, willow branches, and arborvitae leaves were crushed separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of willow branch powder, and 20g of arborvitae leaf powder were mixed and soaked in 850g of 50wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 50wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0137] Comparative Example 7

[0138] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 6 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of sophora japonica twig powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0139] Artemisia argyi, Sophora japonica branches, and Platycladus orientalis leaves were crushed separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of Sophora japonica branch powder, and 20g of Platycladus orientalis leaf powder were mixed and soaked in 850g of 50wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 50wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0140] Comparative Example 8

[0141] This comparative example provides an antibacterial and antioxidant composition, which differs from Example 6 only in that 10g of patchouli powder, 15g of peach twig powder, 15g of willow twig powder, and 15g of sophora japonica twig powder are replaced with 55g of peach twig powder, while the artemisia argyi powder and arborvitae leaf powder remain unchanged. The preparation method specifically includes the following steps:

[0142] Artemisia argyi, peach tree branches, and Platycladus orientalis leaves were crushed separately and passed through a 20-40 mesh sieve. 10g of Artemisia argyi powder, 55g of peach tree branch powder, and 20g of Platycladus orientalis leaf powder were mixed and soaked in 850g of 50wt% ethanol aqueous solution for 0.5-1h. The mixture was heated and refluxed for 1.5h and filtered while hot. The residue was soaked again in 850g of 50wt% ethanol aqueous solution for 0.5-1h and heated and refluxed for 1.5h and filtered while hot. The filtrates were combined, centrifuged, and the supernatant was concentrated. After freezing at -20℃, the mixture was vacuum dried at below 70pa for 24h to obtain the antibacterial and antioxidant composition.

[0143] Performance testing:

[0144] (3) Antibacterial performance test: The antibacterial and antioxidant compositions of Examples 1-6 were replaced with the antibacterial and antioxidant compositions of Comparative Examples 1-8 according to the above test method. The results are shown in Table 3.

[0145] Table 3. Antibacterial properties of the antibacterial and antioxidant compositions of Comparative Examples 1-8.

[0146] sample Staphylococcus aureus (MIC) Escherichia coli (MIC) Comparative Example 1 2mg / mL 8mg / mL Comparative Example 2 1mg / mL 8mg / mL Comparative Example 3 2mg / mL 8mg / mL Comparative Example 4 1mg / mL 8mg / mL Comparative Example 5 2mg / mL 8mg / mL Comparative Example 6 500 μg / mL 8mg / mL Comparative Example 7 1mg / mL 8mg / mL Comparative Example 8 1mg / mL 8mg / mL

[0147] By comparing Examples 5-6 in Table 1 and Comparative Examples 1-8 in Table 3, it can be seen that in the antibacterial and antioxidant composition obtained by extracting the raw materials of the composition using 40-50wt% ethanol aqueous solution, the combination of extracts of patchouli, peach twigs, willow twigs and sophora japonica twigs has a synergistic antibacterial effect on Staphylococcus aureus and Escherichia coli.

[0148] (4) Antioxidant performance test: The antibacterial and antioxidant compositions of Examples 1-6 were replaced with the antibacterial and antioxidant compositions of Comparative Examples 1-8 according to the above test method. The results are shown in Table 4.

[0149] Table 4. Half-maximal inhibitory concentrations of DPPH free radical scavenging activity of the antibacterial and antioxidant compositions of Comparative Examples 1-8.

[0150] sample IC50 (mg / mL) Comparative Example 1 0.0269 Comparative Example 2 0.0226 Comparative Example 3 0.0283 Comparative Example 4 0.0191 Comparative Example 5 0.0281 Comparative Example 6 0.0198 Comparative Example 7 0.0268 Comparative Example 8 0.0190

[0151] As shown in Tables 1-4, the antibacterial and antioxidant composition obtained by extracting the raw materials of the composition using 40-50 wt% ethanol aqueous solution contains extracts of patchouli, peach tree branches, willow tree branches, and sophora tree branches. These extracts not only have a synergistic antibacterial effect against Staphylococcus aureus and Escherichia coli, but also a synergistic scavenging effect against DPPH free radicals.

[0152] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that after reading this application specification, they can still modify or make equivalent substitutions to the specific implementation of the present invention, but these modifications or changes do not depart from the protection scope of the pending claims of the present invention.

Claims

1. A bacteriostatic antioxidant composition characterized in that, The bacteriostatic antioxidant composition is prepared by the method of the following steps: The Artemisia argyi, Agastache rugosa, peach tree branch, willow branch, Sophora japonica branch and Platycladus orientalis are respectively crushed, sieved, and the Artemisia argyi powder, Agastache rugosa powder, peach tree branch powder, willow branch powder, Sophora japonica branch powder and Platycladus orientalis powder are mixed, a solvent is added for soaking, and heating reflux extraction is performed, and the filtrate is filtered while hot, centrifuged, and the supernatant is concentrated and dried to obtain the bacteriostatic antioxidant composition. The solvent is selected from 40-50wt% ethanol aqueous solution.

2. The bacteriostatic antioxidant composition of claim 1, wherein, The mass ratio of the Artemisia argyi powder, Agastache rugosa powder, peach tree branch powder, willow branch powder, Sophora japonica branch powder and Platycladus orientalis powder is 0.8-1.2:0.8-1.2:1.2-1.8:1.2-1.8:1.2-1.8:1.7-2.

3.

3. The bacteriostatic antioxidant composition of claim 1, wherein, The total mass of the Artemisia argyi powder, Agastache rugosa powder, peach tree branch powder, willow branch powder, Sophora japonica branch powder and Platycladus orientalis powder and the mass of the solvent are in the ratio of 1:10-20.

4. The bacteriostatic antioxidant composition of claim 1, wherein, The soaking time is 1-2h. The heating reflux extraction time is 1.5-3h.

5. The bacteriostatic antioxidant composition of claim 1, wherein, The sieving is through a 20-40 mesh sieve.

6. An anti-hair loss shampoo, characterized by, 0.1-0.7wt% of the bacteriostatic antioxidant composition of any one of claims 1-5 and 99.3-99.9wt% of adjuvant.

7. A method of preparing the hair loss-preventing shampoo according to claim 6, characterized by, The method comprises the following steps: The aqueous solution of the bacteriostatic antioxidant composition and the adjuvant are mixed and stirred uniformly to obtain the anti-hair loss shampoo.

8. The method of claim 7, wherein the anti-hair loss shampoo is prepared by adding the ingredients in the order of the ingredients listed in claim 7. The mass concentration of the aqueous solution of the bacteriostatic antioxidant composition is 1-7mg / mL.

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