A hyperspectral microscopic imaging system

Abstract

This invention relates to a hyperspectral microscopic imaging system, comprising a front imaging module and a hyperspectral back imaging module; the front imaging module is used to collect sample light signals and image a front image beam; the hyperspectral back imaging module is used to disperse the front image beam along the dispersion direction to obtain a spatial spectral image; it includes: a magnifying lens group, a segmentation component, and a dispersive lens group; the front image beam is magnified into a magnified image in the dispersion direction by the magnifying lens group, and the segmentation component is disposed on the image plane of the magnified image; the segmentation component consists of multiple microlens groups arranged side by side along the dispersion direction covering the range of the magnified image; the dispersive lens group includes a collimating lens and a converging lens with the same focal length, the collimating lens and the converging lens forming a relay lens group, and a dispersive prism is disposed between them along the dispersion direction. The hyperspectral microscopic imaging system provided by this invention magnifies the multispectral superposition spatial image obtained by the front imaging module through a magnifying lens group, and then divides and converges it into discrete sub-images through a segmentation component, creating camera detection resources for recording spectral information. The dissociated spectral information is integrated into the spatial information and recorded by the camera to obtain a spatial-spectral image without superposition crosstalk. While achieving spatial-spectral separation, it reduces the distortion of spatial information.

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