Screening, construction and application of biosensor for detecting explosive molecules under high-salt condition
By introducing the promoter mutant yqjFas and the reporter gene GFP, YFP or DsRed into the microbial sensor, a biosensor was constructed, which solved the problem of DNT detection in high-salt environment, achieved high-sensitivity detection in high-salt environment, and filled the technical gap in explosive detection in high-salt environment.
CN120796265APending Publication Date: 2025-10-17QINGDAO AGRI UNIV
Patent Information
- Application Number
- CN202510933127.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-07
- Publication Date
- 2025-10-17
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Figure CN120796265A_ABST
Abstract
Aiming at the current situation of DNT detection in a high-salt environment in the prior art, the invention firstly provides a promoter mutant yqjFas for explosive detection in the high-salt environment; the nucleotide sequence of the mutant yqjFas is as shown in SEQ ID NO: 1. And the explosive is 2, 4-dinitrotoluene. The inventor finds that the mutant yqjFas can sense trace DNT under the condition of high-concentration salt content (5-7wt% NaCl), and unexpected technical effects are achieved. On the basis, the invention also provides a biosensor which has salt tolerance and can sense trace explosives, the biosensor adopts GFP, YFP or DsRed as a downstream reporter gene, direct observation can be realized without adding auxiliary factors, and the operation is simple and convenient. The invention further provides a method for rapidly detecting explosives by adopting the biosensor, the technical blank of detecting the explosives in the high-salt environment is filled, and the biosensor has important practical application value.
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Citation Information
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