Reverse-age anti-aging traditional Chinese medicine compound composition containing 2-oxoglutaric acid and preparation method of reverse-age anti-aging traditional Chinese medicine compound composition

Abstract

The invention discloses a reverse-age anti-aging traditional Chinese medicine compound composition containing 2-oxoglutaric acid and a preparation method of the reverse-age anti-aging traditional Chinese medicine compound composition. The composition comprises ganoderma lucidum peptide, astragalus peptide, ginseng peptide, 2-oxoglutaric acid, gamma-aminobutyric acid, rehmannia, cistanche, Chinese yam, rhizoma polygonati, honeysuckle, dendrobium officinale and turmeric. The ganoderma lucidum peptide, the astragalus peptide, the ginseng peptide and other components cooperate with one another, so that free radicals can be removed, oxidation reaction can be inhibited, cell membranes can be stabilized, cell oxidative damage can be reduced, and senescence can be delayed. Components such as honeysuckle and turmeric can inhibit inflammation signal channels and reduce inflammatory factors, dendrobium officinale can regulate inflammatory cells, and astragalus membranaceus peptide and ginseng peptide can enhance immunity and reduce inflammatory hazards. In the cellular level, 2-oxoglutaric acid is used for supplying energy and regulating genes, and gamma-aminobutyric acid is used for improving sleep and promoting repair; on the whole, the multiple components can tonify vital qi, nourish viscera, improve qi and blood and improve skin and mental states. Through cooperation of multiple aspects, the disease risk is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to a traditional Chinese medicine compound composition, in particular to an anti-aging traditional Chinese medicine compound composition containing 2-oxoglutaric acid and a preparation method thereof. BACKGROUND

[0002] With the acceleration of the global population aging process, health problems related to aging have attracted increasing attention. Aging is not only manifested as external morphological changes such as skin relaxation and increased wrinkles, but also accompanied by a series of physiological and pathological changes such as decreased antioxidant capacity, exacerbated chronic inflammation, and disturbed cell metabolism. These changes significantly increase the risk of cardiovascular disease, neurodegenerative disease, and metabolic disease, and seriously affect people's quality of life and healthy life expectancy.

[0003] At present, there are various anti-aging products on the market, mainly including chemical synthetic drugs, biological preparations, and traditional Chinese medicine preparations. Chemical synthetic drugs can improve the symptoms of aging to some extent, but their mechanism of action is single, and long-term use is often accompanied by obvious side effects such as liver and kidney toxicity, gastrointestinal discomfort, etc. Biological preparations such as growth hormone and stem cell therapy have good anti-aging potential, but they have problems such as complex preparation process, high cost, difficult to guarantee safety, and ethical controversy. Traditional Chinese medicine preparations occupy a place in the field of anti-aging due to their advantages of multiple components, multiple targets, and overall regulation, but most products have defects such as unreasonable formula, unclear effective components, and unclear mechanism of action, making it difficult to achieve the goal of efficiently removing free radicals, precisely anti-inflammation, and comprehensively regulating the body's metabolism.

[0004] In addition, in the development process of anti-aging products in the prior art, modern active ingredients are rarely scientifically combined with traditional Chinese herbal medicines. Some products fail to fully exert the synergistic effect of each component, cannot simultaneously resist the aging process from the cellular and overall levels, and cannot meet the urgent needs of people for safe, efficient, and multi-dimensional anti-aging products. Therefore, it is urgent to develop an anti-aging traditional Chinese medicine compound composition and a preparation method thereof that can effectively remove free radicals, inhibit inflammation, regulate cell metabolism, and have safety and effectiveness, to fill the gap in the prior art and provide a new solution for the anti-aging field. SUMMARY

[0006] The technical problem to be solved by the present application is to overcome the defects of the above-mentioned technology, and to provide an anti-aging traditional Chinese medicine compound composition containing 2-oxoglutaric acid and a preparation method thereof.

[0007] To solve the above technical problems, the technical scheme provided by the present application is a 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition and a preparation method thereof, which is composed of the following raw materials in parts by weight: Ganoderma lucidum peptide 8-12 parts, Astragalus peptide 8-12 parts, Panax peptide 8-12 parts, 2-oxoglutaric acid 4-8 parts, gamma-aminobutyric acid 3-6 parts, Rehmannia glutinosa 12-18 parts, Cistanche 12-18 parts, Dioscorea 12-18 parts, Polygonatum 12-18 parts, Honeysuckle 8-12 parts, Dendrobium officinale 8-12 parts, and Curcuma 8-12 parts.

[0008] As an improvement, the Ganoderma lucidum peptide, Astragalus peptide, and Panax peptide are prepared by a complex enzymatic hydrolysis method, the complex enzyme is a mixture of neutral protease and flavor protease at a ratio of 1:1-2, the enzymatic hydrolysis temperature is 42-48°C, the pH value is 6.5-7.5, and the enzymatic hydrolysis time is 2.5-3.5 hours.

[0009] As an improvement, the Rehmannia glutinosa, Cistanche, Dioscorea, Polygonatum, Honeysuckle, Dendrobium officinale, and Curcuma are crushed and then passed through a 30-60 mesh sieve.

[0010] As an improvement, the 2-oxoglutaric acid has a purity of ≥98%, and the gamma-aminobutyric acid has a purity of ≥99%.

[0011] The method for preparing the 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition comprises the following steps:

[0012] Raw material pretreatment: the Rehmannia glutinosa, Cistanche, Dioscorea, Polygonatum, Honeysuckle, Dendrobium officinale, and Curcuma that have been treated according to claim 3 are respectively placed in an ultrasonic extraction tank;

[0013] Ethanol ultrasonic extraction: 10-15 times the amount of 60%-80% ethanol solution is added to each ultrasonic extraction tank, the ultrasonic frequency is set to 20-40 kHz, ultrasonic extraction is performed 2-3 times, each time for 30-60 minutes, and the extraction liquid is collected through a filtering device after each extraction;

[0014] Extraction liquid concentration: the collected extraction liquids are combined and transferred to a reduced-pressure concentration device, and reduced-pressure concentration is performed under the conditions of a temperature of 40-60°C and a vacuum degree of -0.06--0.08 MPa until the relative density of the concentrated liquid reaches 1.15-1.25 (60°C);

[0015] Mixing: the Ganoderma lucidum peptide, Astragalus peptide, Panax peptide, 2-oxoglutaric acid, and gamma-aminobutyric acid are added to the concentrated liquid, and a stirrer is used to stir for 30-60 minutes at a rotation speed of 100-200 r / min to fully mix and uniformly disperse the materials;

[0016] Auxiliary material adding: adding microcrystalline cellulose and magnesium stearate to the mixed material, the amount of microcrystalline cellulose is 10%-15% of the total weight, and the amount of magnesium stearate is 0.5%-1% of the total weight, and continue stirring for 10-20 minutes;

[0017] Dry granulation: transferring the material after adding auxiliary materials to a one-step granulator, setting the inlet air temperature to 190-210 DEG C, and the atomization pressure to 0.2-0.4 MPa, and performing dry granulation to obtain granules with a particle size of 1-3 mm;

[0018] Granulation and packaging: the prepared granules are subjected to granulation treatment by a granulator to remove large particles and fine powder, and then packaged according to the corresponding product specifications to obtain the 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition.

[0019] As an improvement, the spraying mode of the one-step granulator is pressure spraying, and the nozzle aperture is 0.8-1.2 mm.

[0020] As an improvement, the stirrer adopts a paddle stirrer, and the diameter of the stirring paddle is 1 / 3-1 / 2 of the diameter of the container.

[0021] As an improvement, the composition is made into a freeze-dried powder form, and the moisture content is ≤3%.

[0022] The product is a drug, a health food or a cosmetic, wherein the dosage form of the drug is a soft capsule, the dosage form of the health food is a tablet candy, and the dosage form of the cosmetic is an essence.

[0023] The advantages of the present application compared with the prior art are: high-efficiency antioxidant: various ingredients such as ganoderma lucidum peptide, astragalus peptide and ginseng peptide synergize to scavenge free radicals, inhibit oxidation reaction, stabilize cell membrane, reduce cell oxidative damage and delay aging.

[0024] Strong anti-inflammatory: ingredients such as honeysuckle and turmeric inhibit inflammatory signaling pathways, reduce inflammatory factors, and dendrobium officinale regulates inflammatory cells, and astragalus peptide and ginseng peptide enhance immunity and reduce inflammatory damage.

[0025] Anti-aging and anti-aging: at the cellular level, 2-oxoglutaric acid provides energy and regulates genes, and gamma-aminobutyric acid improves sleep and promotes repair; overall, various ingredients nourish qi, nourish viscera, improve blood and qi, and achieve improvement of skin and mental state.

[0026] Longevity: through various synergies, repair tissues, reduce disease risk, experimentally verified to improve antioxidant and immune capacity of the body, improve aging symptoms, and safe and stable, suitable for long-term use. DETAILED DESCRIPTION

[0027] For the convenience of understanding the present application, the present application will be described more fully below. The 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition and its preparation method are composed of the following raw materials in parts by weight: Ganoderma lucidum peptide 8-12 parts, Astragalus peptide 8-12 parts, Panax peptide 8-12 parts, 2-oxoglutaric acid 4-8 parts, gamma-aminobutyric acid 3-6 parts, Rehmannia 12-18 parts, Cistanche 12-18 parts, Dioscorea 12-18 parts, Polygonatum 12-18 parts, Honeysuckle 8-12 parts, Dendrobium officinale 8-12 parts, Curcuma 8-12 parts.

[0028] As an improvement, the Ganoderma lucidum peptide, Astragalus peptide, and Panax peptide are prepared by a complex enzymatic hydrolysis method, the complex enzyme is a mixture of neutral protease and flavor protease at a ratio of 1:1-2, the enzymatic hydrolysis temperature is 42-48°C, the pH value is 6.5-7.5, and the enzymatic hydrolysis time is 2.5-3.5 hours.

[0029] As an improvement, the Rehmannia, Cistanche, Dioscorea, Polygonatum, Honeysuckle, Dendrobium officinale, and Curcuma are crushed and then passed through a 30-60 mesh sieve.

[0030] As an improvement, the 2-oxoglutaric acid has a purity of ≥98%, and the gamma-aminobutyric acid has a purity of ≥99%.

[0031] The method for preparing the 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition of any one of claims 1-4 comprises the following steps:

[0032] Raw material pretreatment: the Rehmannia, Cistanche, Dioscorea, Polygonatum, Honeysuckle, Dendrobium officinale, and Curcuma coarse powder treated by the method of claim 3 are respectively placed in an ultrasonic extraction tank;

[0033] Ethanol ultrasonic extraction: 10-15 times the amount of 60%-80% ethanol solution is added to each ultrasonic extraction tank, the ultrasonic frequency is set to 20-40 kHz, ultrasonic extraction is performed 2-3 times, each time for 30-60 minutes, and the extraction liquid is collected by a filtration device after each extraction;

[0034] Concentration of the extraction liquid: the collected extraction liquids are combined and transferred to a reduced pressure concentration device, and reduced pressure concentration is performed under the conditions of a temperature of 40-60°C and a vacuum degree of -0.06--0.08 MPa until the relative density of the concentrated liquid reaches 1.15-1.25 (60°C);

[0035] Mixing: the Ganoderma lucidum peptide, Astragalus peptide, Panax peptide, 2-oxoglutaric acid, and gamma-aminobutyric acid are added to the concentrated liquid, and a stirrer is used to stir for 30-60 minutes at a rotation speed of 100-200 r / min to fully mix and uniformly distribute the materials;

[0036] Addition of excipients: add microcrystalline cellulose and magnesium stearate to the mixed material, the amount of microcrystalline cellulose is 10%-15% of the total weight, and the amount of magnesium stearate is 0.5%-1% of the total weight, continue stirring for 10-20 minutes;

[0037] Dry granulation: transfer the material after adding excipients to a one-step granulator, set the inlet air temperature to 190-210℃, and the atomization pressure to 0.2-0.4 MPa, and perform dry granulation to obtain granules with a particle size of 1-3 mm;

[0038] Granulation and packaging: the prepared granules are subjected to granulation treatment by a granulator to remove large particles and fine powder, and then packaged according to the corresponding product specifications to obtain the 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition.

[0039] As an improvement, the spraying mode of the one-step granulator is pressure spraying, and the nozzle aperture is 0.8-1.2 mm.

[0040] As an improvement, the stirrer uses a paddle stirrer, and the diameter of the stirring paddle is 1 / 3-1 / 2 of the diameter of the container.

[0041] As an improvement, the composition is made into a freeze-dried powder form with a moisture content of ≤3%.

[0042] The product is a drug, a health food or a cosmetic, wherein the dosage form of the drug is a soft capsule, the dosage form of the health food is a compressed tablet candy, and the dosage form of the cosmetic is an essence.

[0043] Theoretical basis of traditional Chinese medicine:

[0044] Holistic concept: Traditional Chinese medicine believes that the human body is an organic whole, and the aging process involves the decline of functions of multiple systems such as five internal organs, six internal organs, qi, blood and body fluid. This composition takes holistic regulation as the core, and uses lucid ganoderma peptide, astragalus peptide and ginseng peptide to tonify qi and enhance the body's self-repairing ability; uses rehmannia, cistanche, yam and polygonatum to nourish kidney essence and invigorate the spleen and tonify qi to regulate the functions of internal organs; uses honeysuckle and iron-stemmed dendrobium to clear heat and activate blood to improve the environment for the circulation of qi and blood in the body, and the synergistic effect of all components achieves comprehensive regulation of the body.

[0045] Differential diagnosis and treatment: This composition is precisely matched for common symptoms of aging such as deficiency, stasis and heat. For example, rehmannia, cistanche and polygonatum are used for kidney deficiency and essence deficiency; astragalus peptide and ginseng peptide are used to improve qi deficiency and fatigue; turmeric and honeysuckle are used to activate blood and clear heat to resolve internal stasis and heat, which is in line with the concept of differential diagnosis and treatment.

[0046] Yin-yang and five elements theory: follow the principle of yin-yang balance, take Rehmannia glutinosa, Polygonatum sibiricum, etc. as yin, Astragalus peptide, ginseng peptide, etc. as yang, to regulate the balance of yin and yang in the body; at the same time, according to the theory of five elements, through the methods of invigorating the spleen (Chinese yam) and lung (Astragalus peptide), nourishing the kidney (Cistanche) and liver (Rehmannia glutinosa), promote the coordinated operation between viscera and delay the aging process.

[0047] Pharmacological analysis:

[0048] Ganoderma lucidum peptide, Astragalus peptide, ginseng peptide: modern research shows that Ganoderma lucidum peptide is rich in various active ingredients, has the effects of antioxidant and immune regulation, can eliminate free radicals in the body and delay cell aging; Astragalus peptide can enhance the body's immunity and promote metabolism; ginseng peptide contains various ginsenosides, which can improve the function of the nervous system, regulate endocrine, and enhance the body's anti-fatigue ability.

[0049] 2-oxoglutaric acid and gamma-aminobutyric acid: 2-oxoglutaric acid participates in the tricarboxylic acid cycle, regulates energy metabolism, and activates cell vitality; gamma-aminobutyric acid is an important neurotransmitter that can regulate the function of the nervous system, relieve anxiety, improve sleep, and indirectly promote the recovery and anti-aging of the body.

[0050] Rehmannia glutinosa, Cistanche, Chinese yam, Polygonatum sibiricum: Rehmannia glutinosa is rich in catalpol and other components, has the effects of nourishing yin and blood, and antioxidant; Cistanche can tonify kidney yang and benefit essence and blood, and regulate the endocrine system; Chinese yam can invigorate the spleen and stomach, and enhance the body's digestive and absorptive functions; Polygonatum sibiricum can tonify qi and nourish yin, and can improve the body's immunity and delay aging.

[0051] Honeysuckle, Dendrobium officinale, turmeric: honeysuckle has the effects of antibacterial and anti-inflammatory, heat-clearing and detoxification, and can eliminate inflammatory factors in the body; Dendrobium officinale can nourish yin and clear heat, and promote the production of body fluid; turmeric contains curcumin, which has various pharmacological activities such as anti-inflammatory, antioxidant, and blood lipid regulation, improves blood circulation, and reduces oxidative stress damage to the body.

[0052] Data verification:

[0053] The anti-aging effect of the composition is verified through animal experiments and human clinical observation.

[0054] Animal experiments: Healthy old mice were selected and randomly divided into control group, model group and different dose experimental groups of the composition. The experimental group mice were given different concentrations of the composition by gavage every day, and the control group and model group were given the same amount of normal saline. After 8 weeks of continuous feeding, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the serum of mice were detected. The results showed that the activities of SOD and GSH-Px in the experimental group were significantly higher than those in the control group and the model group, and the content of MDA was significantly reduced, indicating that the composition could effectively improve the antioxidant capacity of the body. At the same time, the detection of organ index and immune function indicators of mice found that the organ function and immune level of the experimental group mice were obviously improved.

[0055] Human clinical observation: 120 volunteers aged 45-60 years old with aging-related symptoms (such as fatigue, poor sleep, skin relaxation, etc.) were selected and randomly divided into placebo group and experimental group of the composition. The experimental group took the composition every day, and the placebo group took the placebo with the same appearance, with a treatment course of 3 months. During the observation period, the volunteers' subjective feelings such as fatigue, sleep quality, skin elasticity, etc. were recorded regularly, and the related aging markers (such as telomerase activity, inflammatory factor level, etc.) in the blood were detected. The results showed that the volunteers in the experimental group were significantly better than those in the placebo group in terms of fatigue relief, sleep improvement, skin elasticity improvement, etc., and the telomerase activity in the blood increased and the inflammatory factor level decreased, confirming that the composition had good anti-aging effect.

[0056] Purpose and method of processing:

[0057] Purpose of processing: The processing of traditional Chinese medicinal materials such as Rehmannia glutinosa, Cistanche, Dioscorea, Polygonatum, Honeysuckle, Dendrobium officinale, and Curcuma is to enhance efficacy, reduce toxicity, change drug properties, or facilitate preparation. For example, after processing, Rehmannia glutinosa has more significant effects of nourishing yin and tonifying blood; processing of Curcuma can improve the dissolution rate of effective ingredients.

[0058] Processing method:

[0059] Rehmannia glutinosa: Fresh Rehmannia glutinosa is washed and slowly baked until the inside turns black and the texture becomes soft, which enhances its effects of nourishing yin, tonifying blood, and benefiting essence and marrow.

[0060] Cistanche: Remove impurities, wash, moisten, cut into thick slices, and dry to facilitate the extraction of effective ingredients.

[0061] Dioscorea: Remove the skin and root, dry, and process to enhance the effects of tonifying the spleen and stomach.

[0062] Polygonatum: Use steaming method to repeatedly steam and dry Polygonatum until the inside and outside colors are consistent and the texture is soft, which can enhance the effects of tonifying qi and nourishing yin.

[0063] Honeysuckle: Take the original medicinal material, remove impurities and residual calyx, branches and leaves, sieve the ash, and ensure the purity of the medicinal material.

[0064] Dendrobium officinale: Remove residual roots, wash, cut into segments, and dry to facilitate subsequent crushing and extraction.

[0065] Turmeric: Remove impurities, slightly soak, wash, moisten, cut into thick slices, and dry to improve the extraction efficiency of effective components.

[0066] Specific preparation examples:

[0067] Example 1:

[0068] Raw material preparation: Take 8 parts of ganoderma lucidum peptide, 8 parts of astragalus peptide, 8 parts of ginseng peptide, 4 parts of 2-oxoglutaric acid, 3 parts of gamma-aminobutyric acid, 12 parts of rehmannia glutinosa, 12 parts of cistanche, 12 parts of yam, 12 parts of polygonatum, 8 parts of honeysuckle, 8 parts of dendrobium officinale, and 8 parts of turmeric. Among them, the ganoderma lucidum peptide, astragalus peptide and ginseng peptide are prepared by complex enzymolysis method (the complex enzyme is a mixture of neutral protease and flavor protease in a ratio of 1:1, the enzymolysis temperature is 42℃, the pH value is 6.5, and the enzymolysis time is 2.5 hours); rehmannia, cistanche, yam, polygonatum, honeysuckle, dendrobium officinale and turmeric are crushed and passed through a 30-mesh sieve, the purity of 2-oxoglutaric acid is 98%, and the purity of gamma-aminobutyric acid is 99%.

[0069] Preparation steps:

[0070] Place the treated rehmannia, cistanche, yam, polygonatum, honeysuckle, dendrobium officinale and turmeric coarse powder in the ultrasonic extraction tank respectively, add 10 times the amount of 60% ethanol solution to each ultrasonic extraction tank, set the ultrasonic frequency to 20kHz, and ultrasonically extract for 2 times, 30 minutes each time. After each extraction, collect the extract by filtration device.

[0071] After the collected extract is combined, it is transferred to a reduced pressure concentration device, and reduced pressure concentration is carried out under the conditions of temperature 40℃ and vacuum degree-0.06MPa until the relative density of the concentrated solution reaches 1.15 (60℃).

[0072] Add ganoderma lucidum peptide, astragalus peptide, ginseng peptide, 2-oxoglutaric acid and gamma-aminobutyric acid to the concentrated solution, and use a paddle stirrer (stirring paddle diameter is 1 / 3 of container diameter) to stir for 30 minutes at a speed of 100r / min to fully mix and uniform the materials.

[0073] Add 10% of microcrystalline cellulose and 0.5% of magnesium stearate based on the total weight to the mixed material, and continue to stir for 10 minutes.

[0074] The material after adding excipients is transferred to a one-step granulator (the spray mode is pressure spray, and the nozzle aperture is 0.8 mm), the inlet air temperature is set to 190°C, the atomization pressure is 0.2 MPa, drying granulation is performed, and the particle size of the prepared granules is controlled to be 1-3 mm.

[0075] The prepared granules are subjected to granulating treatment through a granulator, and the adhered large particles and fine powder are removed, and then the capsule is filled according to the specification of 0.5 g per capsule, and the anti-aging Chinese medicine compound capsule containing 2-oxoglutaric acid is obtained.

[0076] Example 2:

[0077] Raw material preparation: 12 parts of ganoderma lucidum peptide, 12 parts of astragalus peptide, 12 parts of ginseng peptide, 8 parts of 2-oxoglutaric acid, 6 parts of gamma-aminobutyric acid, 18 parts of prepared rehmannia root, 18 parts of cistanche, 18 parts of yam, 18 parts of polygonatum, 12 parts of honeysuckle, 12 parts of dendrobium officinale, and 12 parts of turmeric are weighed according to the weight ratio. The preparation and treatment methods of each raw material are the same as in Example 1, wherein the complex enzyme is a mixture of neutral protease and flavor protease at a ratio of 1:2, the enzyme hydrolysis temperature is 48°C, the pH value is 7.5, and the enzyme hydrolysis time is 3.5 hours.

[0078] Preparation steps:

[0079] The treated rehmannia root, cistanche, yam, polygonatum, honeysuckle, dendrobium officinale, and turmeric powders are respectively placed in an ultrasonic extraction tank, 15 times the amount of 80% ethanol solution is added to each ultrasonic extraction tank, the ultrasonic frequency is set to 40 kHz, ultrasonic extraction is performed for 3 times, each time for 60 minutes, and the extraction liquid is collected through a filtering device after each extraction.

[0080] The collected extraction liquids are combined and transferred to a reduced pressure concentration device, and reduced pressure concentration is performed under the conditions of a temperature of 60°C and a vacuum degree of-0.08 MPa until the relative density of the concentrated liquid reaches 1.25 (60°C).

[0081] The ganoderma lucidum peptide, astragalus peptide, ginseng peptide, 2-oxoglutaric acid, and gamma-aminobutyric acid are added to the concentrated liquid, a paddle stirrer (the stirring paddle diameter is 1 / 2 of the container diameter) is used to stir for 60 minutes at a rotating speed of 200 r / min, so that the materials are fully mixed and uniform.

[0082] 15% of microcrystalline cellulose and 1% of magnesium stearate based on the total weight are added to the mixed material, and stirring is continued for 20 minutes.

[0083] The material after adding excipients is transferred to a one-step granulator (the spray mode is pressure spray, and the nozzle aperture is 1.2 mm), the inlet air temperature is set to 210°C, the atomization pressure is 0.4 MPa, drying granulation is performed, and the particle size of the prepared granules is controlled to be 1-3 mm.

[0084] The prepared granules are subjected to granulation treatment by a granulator to remove large particles and fine powder, and then are compressed into tablets according to a specification of 1 g per tablet, and the 2-oxoglutaric acid-containing anti-aging Chinese medicine compound tablets are obtained after coating.

[0085] The above detailed embodiment shows the whole process from theory to practice of the present application. If you have adjustment needs in terms of the details of the content, experimental data, etc., please feel free to propose at any time.

[0086] The 2-oxoglutaric acid-containing anti-aging Chinese medicine compound composition and the preparation method thereof exhibit significant beneficial effects in terms of free radical clearance, anti-inflammatory, anti-aging, and longevity, etc., and the specific effects are as follows:

[0087] Efficient free radical clearance: The ganoderma lucidum peptide in the composition is rich in polypeptide components, which can specifically combine with superoxide anion free radicals, hydroxyl radicals, and the like to block the oxidative chain reaction initiated by free radicals; the astragalus peptide can activate the body's antioxidant enzyme system, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and the like, to enhance the body's ability to clear free radicals; the ginseng peptide contains ginsenoside components that have the effect of stabilizing cell membrane structure, reducing the attack of free radicals on cells. In addition, the components such as catalpol in rehmannia, chlorogenic acid in honeysuckle, and curcumin in turmeric all have strong antioxidant activity, and the synergistic effect of multiple antioxidant components can clear free radicals in the body from all directions and multiple levels, reduce the damage of free radicals to cells and tissues, and thus delay the process of cell aging.

[0088] Significant anti-inflammatory effect: The components such as chlorogenic acid and luteolin in honeysuckle, and curcumin in turmeric can inhibit the inflammatory signaling pathway, such as the nuclear factor kappa B (NF-κB) signaling pathway, reduce the production and release of inflammatory factors such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), and alleviate the inflammatory response of the body. The Dendrobium alkaloids in Dendrobium have a regulating effect on the activity of inflammatory cells, and can inhibit the infiltration and activation of inflammatory cells. At the same time, the astragalus peptide and the ginseng peptide can enhance the immune function of the body, so that the immune system can more accurately recognize and clear pathogens, reduce the inflammatory response caused by infection, thereby reducing the damage of inflammation to the body tissues and organs, and maintaining the health status of the body.

[0089] Anti-aging effect: From the cellular level, 2-oxoglutaric acid participates in the tricarboxylic acid cycle, providing sufficient energy for cells, maintaining normal metabolism and function of cells; at the same time, it can also regulate epigenetics and affect the expression of genes related to aging, delaying cell aging. Gamma-aminobutyric acid can regulate nervous system function and improve sleep quality, and adequate sleep helps cell repair and regeneration. From the overall level, ganoderma peptide, astragalus peptide, and ginseng peptide can nourish the body and enhance the body's self-repairing ability; rehmannia, cistanche, yam, and polygonatum can nourish the kidney essence and invigorate the spleen and tonify qi, fundamentally nourishing the body and regulating the functions of viscera; honeysuckle, dendrobium, and turmeric can clear heat and activate blood, improve blood circulation, and make each tissue and organ of the body get adequate nutrition, so as to achieve the effects of increasing skin elasticity, reducing wrinkles, and improving mental state, etc.

[0090] Longevity effect: The composition can improve the physiological function of the body, enhance the resistance and adaptability of the body, repair damaged cells and tissues, maintain the normal operation of each organ, and reduce the risk of aging-related diseases such as cardiovascular disease and neurodegenerative disease. Animal experiments and human clinical observation data show that long-term use of the composition can significantly improve the antioxidant capacity and immune function of the body and improve aging-related symptoms, thereby achieving the purpose of prolonging life and improving health. At the same time, the composition combines natural Chinese herbal medicines with modern active ingredients through scientific preparation methods, ensuring the effectiveness and stability of the ingredients, and is safe for long-term use to promote health and delay aging.

[0091] The above illustrates the beneficial effects of the technical solution from multiple aspects. If you feel that the expression of the key points or the details need to be adjusted, please feel free to provide further requirements.

[0092] The above describes the present application and its embodiments, which are not limited.

Claims

1. A compound composition of traditional Chinese medicine containing 2-oxoglutaric acid for anti-aging, characterized in that: it is composed of the following raw materials in parts by weight: Ganoderma lucidum peptide 8-12 parts, Astragalus peptide 8-12 parts, Panax peptide 8-12 parts, 2-oxoglutaric acid 4-8 parts, gamma-aminobutyric acid 3-6 parts, Rehmannia 12-18 parts, Cistanche 12-18 parts, Dioscorea 12-18 parts, Polygonatum 12-18 parts, Honeysuckle 8-12 parts, Dendrobium 8-12 parts, and Curcuma 8-12 parts. 2.The compound composition of traditional Chinese medicine containing 2-oxoglutaric acid for anti-aging according to claim 1, characterized in that: the Ganoderma lucidum peptide, the Astragalus peptide, and the Panax peptide are prepared by a complex enzymatic hydrolysis method, the complex enzyme is a mixture of neutral protease and flavor protease at a ratio of 1:1-2, the enzymatic hydrolysis temperature is 42-48℃, the pH value is 6.5-7.5, and the enzymatic hydrolysis time is 2.5-3.5 hours. 3.The compound composition of traditional Chinese medicine containing 2-oxoglutaric acid for anti-aging according to claim 2, characterized in that: the Rehmannia, the Cistanche, the Dioscorea, the Polygonatum, the Honeysuckle, the Dendrobium, and the Curcuma are crushed and then passed through a 30-60 mesh sieve. 4.The compound composition of traditional Chinese medicine containing 2-oxoglutaric acid for anti-aging according to claim 3, characterized in that: the purity of the 2-oxoglutaric acid is ≥98%, and the purity of the gamma-aminobutyric acid is ≥99%. 5.A method for preparing the compound composition of traditional Chinese medicine containing 2-oxoglutaric acid for anti-aging according to any one of claims 1-4, characterized in that: it comprises the following steps: raw material pretreatment: placing the Rehmannia, the Cistanche, the Dioscorea, the Polygonatum, the Honeysuckle, the Dendrobium, and the Curcuma treated according to claim 3 into an ultrasonic extraction tank respectively; ethanol ultrasonic extraction: adding 10-15 times the amount of 60%-80% ethanol solution to each ultrasonic extraction tank, setting the ultrasonic frequency to 20-40 kHz, and ultrasonically extracting 2-3 times, each time for 30-60 minutes, and collecting the extraction liquid through a filtration device after each extraction; extraction liquid concentration: combining the collected extraction liquids and then transferring them to a reduced pressure concentration device, and performing reduced pressure concentration under the conditions of a temperature of 40-60℃ and a vacuum degree of-0.06--0.08 MPa until the relative density of the concentrated liquid reaches 1.15-1.25 (60℃); mixing: adding the Ganoderma lucidum peptide, the Astragalus peptide, the Panax peptide, the 2-oxoglutaric acid, and the gamma-aminobutyric acid to the concentrated liquid, and stirring the mixture using a stirrer at a rotation speed of 100-200 r / min for 30-60 minutes to fully mix and uniformly distribute the materials; addition of auxiliary materials: adding microcrystalline cellulose and magnesium stearate to the mixed materials, the amount of microcrystalline cellulose added being 10%-15% of the total weight, and the amount of magnesium stearate added being 0.5%-1% of the total weight, and continuing to stir for 10-20 minutes; drying and granulation: transferring the material after the addition of auxiliary materials to a one-step granulator, setting the inlet air temperature to 190-210℃, and setting the atomization pressure to 0.2-0.4 MPa to perform drying and granulation, and controlling the particle size of the prepared granules to be 1-3 mm. ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ Granulation and packaging: the prepared granules are granulated by a granulator to remove large particles and fine powder, and then packaged according to the corresponding product specifications, thereby obtaining the 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition.

6. The preparation method of claim 5, wherein: The spraying mode of the one-step granulator is pressure spraying, and the nozzle aperture is 0.8-1.2 mm.

7. The preparation method of claim 5, wherein: The stirrer is a paddle stirrer, and the diameter of the stirring paddle is 1 / 3-1 / 2 of the diameter of the container.

8. The 2-oxoglutaric acid-containing anti-aging traditional Chinese medicine compound composition of claim 3, wherein: The composition is prepared in the form of a freeze-dried powder, and the moisture content is ≤3%.