A method for cultivating Xanthoceras sorbifolia Bunge by cutting rooting and seedling growing

Abstract

The present application relates to the technical field of cutting seedling, in particular to a Xanthoceras sorbifolia Bunge cutting rooting seedling breeding method, the cutting rooting seedling breeding method comprises the following steps: (1) cutting substrate preparation; (2) the preparation of rooting agent; (3) cutting selection and preparation; (4) seedbed establishment; (5) cutting pretreatment; (6) cutting; (7) cutting management after cutting; the rooting rate of Xanthoceras sorbifolia Bunge cutting by the method is high, and the survival rate is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cutting seedling, in particular to a Xanthoceras sorbifolia Bunge cutting rooting seedling breeding method. BACKGROUND

[0002] Xanthoceras sorbifolia Bunge is a plant of Sapindaceae Xanthoceras, which is cold-resistant, drought-resistant and saline-alkali-tolerant, and is a woody oil plant widely distributed in northern China. Xanthoceras sorbifolia Bunge has important ecological, economic and medicinal values, but it is easy to produce root sprouts, and grafting propagation is time-consuming and laborious. There are reports on Xanthoceras sorbifolia Bunge asexual reproduction methods such as tissue culture, hardwood cutting, softwood cutting, and root cutting. In the aspect of Xanthoceras sorbifolia Bunge tissue culture, leaves, axillary buds, stem tips, seeds, and cotyledons have been used as explants to induce adventitious buds and roots, and regenerated plants have been obtained. The patent "Xanthoceras sorbifolia Bunge tissue culture rapid propagation method using roots as explants and Xanthoceras sorbifolia Bunge adventitious buds cultured thereby" uses Xanthoceras sorbifolia Bunge roots as explants to obtain Xanthoceras sorbifolia Bunge adventitious buds in a short period of time; the patent "Xanthoceras sorbifolia Bunge asexual rapid propagation method" quickly obtains Xanthoceras sorbifolia Bunge seedlings through zygotic embryo induction, subculture and expansion of somatic embryos, subculture and rooting culture of adventitious buds, and seedling raising. The patent "Method for promoting Xanthoceras sorbifolia Bunge softwood cutting rooting and seedling raising" uses Xanthoceras sorbifolia Bunge softwood treated with 5000 mg / L high-concentration IBA, and under full-sunlight spraying conditions, the rooting and seedling raising of Xanthoceras sorbifolia Bunge softwood cuttings are promoted. Although the above researches provide a basis for Xanthoceras sorbifolia Bunge cutting to some extent, the low rooting rate and long seedling raising period of Xanthoceras sorbifolia Bunge cutting are still technical problems to be solved. SUMMARY

[0003] In view of the deficiencies of the prior art, the present application provides a Xanthoceras sorbifolia Bunge cutting rooting seedling breeding method.

[0004] The present application is realized by the following technical scheme:

[0005] A Xanthoceras sorbifolia Bunge cutting rooting seedling breeding method, comprising the following steps:

[0006] (1) Preparation of cutting substrate:

[0007] (11) The diatomite is dispersed in a 5M hydrochloric acid solution, stirred at room temperature for 8-10 h, washed with deionized water to neutralization after filtration, dried at 80℃, and dispersed in toluene at a ratio of 30 mg / mL, 3-aminopropyltriethoxysilane (APTES) is added dropwise, the temperature is raised to 100℃, and stirred and refluxed for 10-12 h, then the solvent is removed by filtration, washed with ethanol, and dried at 60℃ under vacuum to obtain aminosilica;

[0008] (12) Mix cetyltrimethylammonium bromide (CTAB) and 1 mol / L NaOH solution in ultrapure water, stir at 80℃ for 20 min, drop a mixture of tetraethoxysilane (TEOS) and (3-mercaptopropyl) trimethoxysilane (MPTMS), stir for 10 min under nitrogen atmosphere, filter, wash the filter cake with deionized water and ethanol, vacuum dry, add 0.5 mol / L HCl ethanol solution, reflux at 80℃ for 24 h, filter, wash the filter cake with deionized water, vacuum dry;

[0009] (13) Add the product obtained in step (12) to a 30wt% H2O2 solution at a ratio of 50 mg / mL, oxidize at 30℃ for 24 h, filter and wash with deionized water, add a 10wt% H2SO4 solution at a ratio of 20 mg / mL and stir for 1 h, filter, wash the filter cake with deionized water, vacuum dry, and obtain sulfonic acid-based SiO2;

[0010] (14) Mix the sulfonic acid-based SiO2 obtained in step (13) and the aminated diatomite obtained in step (11), add ultrapure water, stir at 150-200 rpm for 12 h, filter, wash with deionized water, and vacuum dry to obtain modified diatomite. Mix the modified diatomite, river sand, and peat soil at a volume ratio of 1:1:3 to obtain a cutting substrate.

[0011] (2) Preparation of rooting agent:

[0012] (21) Wash and dry liquorice, milkvetch, platycodon and honeysuckle, crush to pass through an 80-100 mesh sieve, mix with 15-20 times the weight volume of deionized water, sterilize at 121℃ for 30 min, cool to room temperature, and inoculate with activated Bacillus amyloliquefaciens and Lactobacillus rhamnosus at an inoculum of 5×10 7 CFU / mL each;

[0013] (22) After inoculation in step (21), culture at 30℃ on a 150-200 rpm shaker for 48 h, centrifuge at 10000 rpm for 15-20 min, pass the supernatant through a 0.22μm filter membrane, mix with indole-3-butyric acid (IBA) at a ratio of 50 mg / L to obtain a rooting agent.

[0014] (3) Selection and preparation of cuttings: In late May, select 2-3 year old Xanthoceras sorbifolia Bunge mother trees, full, straight and free of pests and diseases, cut into 8-12 cm long cuttings, cut flat at the top, cut obliquely at the bottom, and retain 3 full buds per cutting;

[0015] (4) Establishment of seedbed: Sterilize the cutting substrate with a 1000-fold carbendazim solution, fill into a flowerpot (inner diameter 12 cm, height 12 cm) to a thickness of 10 cm;

[0016] (5) The cutting pretreatment: the cutting is completely immersed in the 1000 times carbendazim solution for 3-5 min, and then washed with clean water, and the lower end of the cutting is soaked in the rooting agent for 30 min;

[0017] (6) The cutting: the cutting is inserted into the substrate along the hole with the bamboo stick, the lower end of the cutting is inserted into the substrate, the cutting depth is 3-5 cm, one cutting is inserted into each flowerpot, and the substrate is lightly compacted; and the cutting is poured with water;

[0018] (7) The cutting management: the air humidity is 80%, the substrate humidity is 50%-70%, and the temperature is (25±2) ℃ during the cultivation, and the cutting is sprayed with 0.5wt% carbendazim solution every 7 days.

[0019] Further, in step (11), the mass concentration of the diatomite in the 5M hydrochloric acid solution is 50 mg / mL.

[0020] Further, in step (11), the dosage ratio of the diatomite to APTES is 1 g:3 mL.

[0021] Further, in step (12), the dosage ratio of the CTAB, 1 mol / L NaOH solution and ultrapure water is 0.5 g:3.5 mL:120 mL.

[0022] Further, in step (12), the molar ratio of the CTAB, TEOS and MPTMS is 0.11:0.9:0.1.

[0023] Further, in step (12), the volume ratio of the 0.5 mol / L HCl ethanol solution to ultrapure water is 5:4.

[0024] Further, in step (14), the dosage ratio of the sulfonic acid-based SiO2, the aminated diatomite and ultrapure water is 1 g:3 g:200 mL.

[0025] Further, in step (21), the mass ratio of the liquorice, the astragalus, the platycodon and the honeysuckle is 1:1:3:2.

[0026] Further, in step (21), the bacillus amyloliquefaciens and lactobacillus rhamnosus are purchased from the Guangdong Microbial Digital Culture Collection Center, and the numbers are GDMCC NO. 1.3155 and GDMCC NO. 1.2223 respectively.

[0027] Compared with the prior art, the present application has the following beneficial effects:

[0028] The present application provides a Xingguo fruit cutting rooting seedling breeding method, which improves the rooting rate and survival rate of Xingguo fruit cutting by preparing cutting substrate and rooting agent, effectively solves the problem of low rooting rate of Xingguo fruit cutting in the prior art, and provides a basis for large-scale vegetative propagation of Xingguo fruit. The present application prepares modified diatomite, which is modified by amino silane coupling agent to introduce amino groups, and porous SiO2 microspheres containing thiol groups are prepared by hydrothermal method. The thiol groups are oxidized into sulfonic acid groups, which have electrostatic interaction with the amino groups of the amino-modified diatomite, thereby combining the SiO2 microspheres with the diatomite. The porous structure and functional groups synergistically improve the substrate permeability, and the nutrients are adsorbed by ion exchange, which can slowly release mineral elements, which is beneficial to the growth of cuttings. The diatomite retains its original air permeability and water permeability, and further optimizes the air permeability of the substrate. The present application uses loose and air-permeable river sand, which is supplemented with organic matter by adding peat soil, and cooperates with modified diatomite to build a substrate environment conducive to the germination of Xingguo cutting root system. The present application prepares a rooting agent by fermenting traditional Chinese medicines such as licorice, astragalus, platycodon and honeysuckle with specific microorganisms, and compounding indole butyric acid. The multi-component synergistically promotes rooting. The present application selects Bacillus amyloliquefaciens and Lactobacillus rhamnosus to ferment the mixture of licorice, astragalus, platycodon and honeysuckle. The strains can decompose effective components in traditional Chinese medicines (such as astragalus polysaccharide and glycyrrhizic acid) during metabolism, degrade macromolecular substances such as flavones into small molecular active substances (such as flavone glycoside), making them more easily absorbed by cuttings, and also produce growth hormone analogues, amino acids, vitamins and other secondary metabolites, which synergistically act with the active components of traditional Chinese medicines to improve the rooting rate. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the present application or prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description are only a part of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort.

[0030] Figure 1 The rooting rate after cutting for the present application examples 1-3 and comparative examples 1-3;

[0031] Figure 2 The average number of roots and root length after cutting for the present application examples 1-3 and comparative examples 1-3;

[0032] Figure 3 The survival rate after cutting for the present application examples 1-3 and comparative examples 1-3. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with specific examples, but the present application is not limited to the following examples. It should be noted that, unless otherwise specified, the chemical reagents involved in the present application are purchased through commercial channels.

[0034] Example 1: A Xanthoceras sorbifolia Bunge cutting rooting and seedling growing method, comprising the following steps:

[0035] (1) Preparation of cutting substrate:

[0036] (11) 5 g of diatomite was dispersed in 100 mL of 5M hydrochloric acid solution, stirred at room temperature for 10 h, washed with deionized water to neutral, dried at 80℃, ultrasonically dispersed in toluene at a ratio of 30 mg / mL, 15 mL of 3-aminopropyltriethoxysilane (APTES) was added dropwise, the temperature was raised to 100℃, stirred and refluxed for 12 h, the solvent was removed by filtration, washed with ethanol, and vacuum dried at 60℃ to obtain aminated diatomite;

[0037] (12) 0.5 g of cetyltrimethylammonium bromide (CTAB) and 3.5 mL of 1 mol / L NaOH solution were added to 120 mL of ultrapure water and mixed, stirred at 80℃ for 20 min, then a mixture of tetraethoxysilane (TEOS) and (3-mercaptopropyl)trimethoxysilane (MPTMS) was added dropwise, the molar ratio of CTAB, TEOS and MPTMS was 0.11:0.9:0.1, after 10 min, stirred for 2 h under nitrogen atmosphere, suction filtered, the filter cake was washed with deionized water and ethanol, and vacuum dried, then 150 mL of 0.5 mol / L HCl ethanol solution was added, refluxed at 80℃ for 24 h, suction filtered, the filter cake was washed with deionized water, and vacuum dried;

[0038] (13) The product obtained in step (12) was added to a 30wt% H2O2 solution at a ratio of 50 mg / mL, oxidized at 30℃ for 24 h, filtered and washed with deionized water, then 10wt% H2SO4 solution was added at a ratio of 20 mg / mL and stirred for 1 h, suction filtered, the filter cake was washed with deionized water, and vacuum dried to obtain sulfonic acid-based SiO2;

[0039] (14) 1 g of sulfonic acid-based SiO2 obtained in step (13) and 3 g of aminated diatomite obtained in step (11) were mixed, added to 200 mL of ultrapure water, stirred at 200 rpm for 12 h, suction filtered, washed with deionized water, and vacuum dried to obtain modified diatomite. The modified diatomite, river sand and grass charcoal were mixed at a volume ratio of 1:1:3 to obtain a cutting substrate.

[0040] (2) Preparation of rooting agent:

[0041] (21) Wash and dry liquorice 1 g, milkvetch 1 g, platycodon 3 g and honeysuckle 2 g, crush and pass through a 100 mesh sieve, add 20 times the weight volume of deionized water, sterilize at 121°C for 30 min, cool to room temperature, and inoculate 5x10 7 CFU / mL of activated Bacillus amyloliquefaciens and Lactobacillus rhamnosus, which are purchased from the Guangdong Microbial Culture Collection Center, with numbers GDMCC NO. 1.3155 and GDMCC NO. 1.2223, respectively;

[0042] (22) After inoculation in step (21), culture at 30°C on a 200 rpm shaker for 48 h, centrifuge at 10,000 rpm for 20 min, pass the supernatant through a 0.22 μm filter membrane, and add indolebutyric acid (IBA) at a proportion of 50 mg / L to mix well to obtain a rooting agent.

[0043] (3) Selection and preparation of cuttings: in late May, select 3-year-old Xanthoceras sorbifolia Bunge mother trees, 1-year-old full, straight and disease-free branches, cut into 12 cm long cuttings, cut flat at the upper end, and cut obliquely at the lower end, and retain 3 full buds per cutting;

[0044] (4) Establishment of seedbed: the cutting substrate is sprayed with 1000 times carbendazim solution for disinfection, and is loaded into a flowerpot (inner diameter 12 cm, height 12 cm) with a thickness of 10 cm;

[0045] (5) Pretreatment of cuttings: completely immerse the cuttings in 1000 times carbendazim solution for 5 min, rinse with clean water, and immerse the lower 5 cm of the cuttings in the rooting agent for 30 min;

[0046] (6) Cutting: insert the pre-treated cuttings into the substrate along the hole with a bamboo stick, insert the lower end of the cuttings into the substrate at a depth of 5 cm, insert 1 cutting per flowerpot, and gently compact the substrate. After cutting, water thoroughly;

[0047] (7) Post-cutting management: maintain air humidity at 80% and substrate humidity at 70% during cultivation, and maintain a temperature of (25±2) °C, and spray 0.5wt% carbendazim solution every 7 d after cutting.

[0048] Example 2: A Xanthoceras sorbifolia Bunge cutting rooting and seedling raising method, comprising the following steps:

[0049] (1) Preparation of cutting substrate:

[0050] (11) Diatomite 5 g was dispersed in 100 mL of 5M hydrochloric acid solution, stirred at room temperature for 8 h, washed with deionized water to neutral after filtration, dried at 80℃, ultrasonic dispersed in toluene at a ratio of 30 mg / mL, 3-aminopropyltriethoxysilane (APTES) 15 mL was added dropwise, the temperature was raised to 100℃, stirred and refluxed for 10 h, the solvent was removed by filtration, washed with ethanol, and vacuum dried at 60℃ to obtain aminated diatomite;

[0051] (12) Cetyltrimethylammonium bromide (CTAB) 0.5 g and 1 mol / L NaOH solution 3.5 mL were added to ultrapure water 120 mL and mixed, stirred at 80℃ for 20 min, a mixture of tetraethoxysilane (TEOS) and (3-mercaptopropyl) trimethoxysilane (MPTMS) was added dropwise, the molar ratio of CTAB, TEOS and MPTMS was 0.11:0.9:0.1, after 10 min, stirred for 2 h under nitrogen atmosphere, suction filtration, the filter cake was washed with deionized water and ethanol, vacuum dried, added to 0.5 mol / L HCl ethanol solution 150 mL, refluxed at 80℃ for 24 h, suction filtration, the filter cake was washed with deionized water, vacuum dried;

[0052] (13) The product obtained in step (12) was added to a 30wt% H2O2 solution at a ratio of 50 mg / mL, oxidized at 30℃ for 24 h, filtered and washed with deionized water, added to a 10wt% H2SO4 solution at a ratio of 20 mg / mL and stirred for 1 h, suction filtration, the filter cake was washed with deionized water, vacuum dried, to obtain sulfonic acid group SiO2;

[0053] (14) The sulfonic acid group SiO2 1 g obtained in step (13) and the aminated diatomite 3 g obtained in step (11) were mixed, added to ultrapure water 200 mL, stirred at 150 rpm for 12 h, suction filtration, deionized water washing, vacuum dried, to obtain modified diatomite, the modified diatomite, river sand and grass charcoal were mixed at a volume ratio of 1:1:3 to obtain a cutting substrate.

[0054] (2) Preparation of rooting agent:

[0055] (21) Licorice 1 g, milk vetch 1 g, platycodon 3 g and honeysuckle 2 g were washed and dried, crushed through an 80 mesh sieve, added to 15 times the weight volume of deionized water, mixed, sterilized at 121℃ for 30 min, cooled to room temperature, inoculated with activated Bacillus amyloliquefaciens and Lactobacillus rhamnosus at an inoculum of 5×10 7 CFU / mL; Bacillus amyloliquefaciens and Lactobacillus rhamnosus were purchased from Guangdong Microbial Culture Collection Center, with numbers GDMCC NO. 1.3155 and GDMCC NO. 1.2223, respectively;

[0056] (22) After inoculation of step (21), 30°C, 150 rpm shaker culture for 48 h, 10000 rpm centrifugation for 15 min, the supernatant was filtered through a 0.22 μm filter, and indole butyric acid (IBA) was added at a ratio of 50 mg / L, and mixed to obtain a rooting agent.

[0057] (3) Selection and preparation of cuttings: In late May, select 2-year-old Xanthoceras sorbifolia Bunge mother trees, 1-year-old full, straight and disease-free branches, cut into 8 cm long cuttings, cut flat at the top, and cut obliquely at the bottom, leaving 3 full buds on each cutting;

[0058] (4) Establishment of seedbed: The cutting substrate was sprayed with 1000 times carbendazim solution for disinfection, and was placed in a flowerpot (inner diameter 12 cm, height 12 cm) with a thickness of 10 cm;

[0059] (5) Pretreatment of cuttings: immerse the cuttings in 1000 times carbendazim solution for 3 min, rinse with water, and immerse the lower 5 cm of the cuttings in the rooting agent for 30 min;

[0060] (6) Cutting: Insert the pre-treated cuttings into the substrate along the hole with a bamboo stick, insert the lower end of the cuttings into the substrate at a depth of 3 cm, insert 1 cutting per flowerpot, and gently compact the substrate. After cutting, water thoroughly;

[0061] (7) Post-cutting management: maintain air humidity at 80%, substrate humidity at 50%, temperature at (25±2) °C, and spray 0.5wt% carbendazim solution every 7 days after cutting.

[0062] Example 3: A Xanthoceras sorbifolia Bunge cutting rooting and seedling raising method, comprising the following steps:

[0063] (1) Preparation of cutting substrate:

[0064] (11) Disperse 5 g of diatomite in 100 mL of 5M hydrochloric acid solution, stir at room temperature for 9 h, wash with deionized water until neutral, dry at 80°C, disperse in toluene at a ratio of 30 mg / mL, add 3-aminopropyltriethoxysilane (APTES) 15 mL dropwise, heat to 100°C, stir and reflux for 11 h, filter to remove the solvent, wash with ethanol, and dry at 60°C under vacuum to obtain aminated diatomite;

[0065] (12) Hexadecyltrimethylammonium bromide (CTAB) 0.5 g and 1 mol / L NaOH solution 3.5 mL were added into ultrapure water 120 mL, mixed uniformly, stirred at 80℃ for 20 min, a mixture of tetraethoxysilane (TEOS) and (3-mercaptopropyl) trimethoxysilane (MPTMS) was added dropwise, the molar ratio of CTAB, TEOS and MPTMS was 0.11:0.9:0.1, after 10 min, stirred for 2 h under nitrogen atmosphere, suction filtration, the filter cake was washed with deionized water and ethanol, vacuum dried, added into 0.5 mol / L HCl ethanol solution 150 mL, refluxed at 80℃ for 24 h, suction filtration, the filter cake was washed with deionized water, vacuum dried;

[0066] (13) The product obtained in step (12) was added into 30wt% H2O2 solution at a ratio of 50 mg / mL, oxidized at 30℃ for 24 h, filtered and washed with deionized water, added into 10wt% H2SO4 solution at a ratio of 20 mg / mL and stirred for 1 h, suction filtration, the filter cake was washed with deionized water, vacuum dried, to obtain sulfonic acid-based SiO2;

[0067] (14) The sulfonic acid-based SiO2 1 g obtained in step (13) and the amino-modified diatomite 3 g obtained in step (11) were mixed uniformly, added into ultrapure water 200 mL, stirred at 180 rpm for 12 h, suction filtration, deionized water washing, vacuum drying, to obtain modified diatomite, the modified diatomite, river sand and grass charcoal were mixed uniformly at a volume ratio of 1:1:3, to obtain a cutting substrate.

[0068] (2) Preparation of rooting agent:

[0069] (21) Glycyrrhiza 1 g, Astragalus 1 g, Platycodon 3 g and honeysuckle 2 g were washed and dried, crushed through a 90 mesh sieve, added into 18 times weight volume of deionized water, mixed uniformly, sterilized at 121℃ for 30 min, cooled to room temperature, inoculated with activated Bacillus amyloliquefaciens and Lactobacillus rhamnosus at an inoculum of 5×10 7 CFU / mL; Bacillus amyloliquefaciens and Lactobacillus rhamnosus were purchased from Guangdong Microbial Culture Collection Center, with numbers GDMCC NO. 1.3155 and GDMCC NO. 1.2223, respectively;

[0070] (22) After inoculation in step (21), 30℃, 180 rpm shaking bed culture for 48 h, 10000 rpm centrifugation for 18 min, the supernatant was passed through a 0.22μm filter membrane, added with indole-3-butyric acid (IBA) at a ratio of 50 mg / L, mixed uniformly, to obtain a rooting agent.

[0071] (3) Selection and preparation of cuttings: In late May, 3-year-old Xanthoceras sorbifolia mother trees were selected, and 1-year-old branches that were full, straight, and free of pests and diseases were cut into 10 cm long cuttings. The upper end was cut flat, and the lower end was cut obliquely. Each cutting retained 3 full buds;

[0072] (4) Establishment of seedbed: The cutting substrate was sprayed with 1000 times carbendazim solution for disinfection, and was loaded into a flowerpot (inner diameter 12 cm, height 12 cm) with a thickness of 10 cm;

[0073] (5) Pretreatment of cuttings: The cuttings were completely immersed in 1000 times carbendazim solution for 4 min, rinsed with clean water, and the lower 5 cm of the cuttings were soaked in a rooting agent for 30 min;

[0074] (6) Cutting: A bamboo stick was used to make a hole in the substrate, and the pretreated cuttings were inserted into the substrate along the hole with the lower end inserted into the substrate. The cutting depth was 4 cm, and 1 cutting was inserted into each flowerpot. The substrate was gently compacted, and the cuttings were thoroughly watered after cutting;

[0075] (7) Management after cutting: During the cultivation period, the air humidity was maintained at 80%, the substrate humidity was maintained at 60%, the temperature was (25±2) °C, and 0.5wt% carbendazim solution was sprayed every 7 days after cutting.

[0076] Comparative Example 1 and Example 1 differ only in that the rooting agent is prepared as follows: 1 g of licorice, 1 g of radix astragali, 3 g of platycodon grandiflorum, and 2 g of honeysuckle are washed, dried, and ground to pass through a 100 mesh sieve. 20 times the weight volume of deionized water is added and mixed uniformly. After soaking in deionized water for 1 h, boiling is continued for 30 min. Filtration is performed, and the decoction liquid is obtained. The filtrate is repeated with the above operation, and the two times of decoction liquid are combined. Filtration is performed through a 0.22 μm filter membrane. Indole-3-butyric acid (IBA) is added at a proportion of 50 mg / L and mixed uniformly to obtain the rooting agent.

[0077] Comparative Example 2 and Example 1 differ only in that the rooting agent is prepared as follows: deionized water is filtered through a 0.22 μm filter membrane, and indole-3-butyric acid (IBA) is added at a proportion of 50 mg / L and mixed uniformly to obtain the rooting agent.

[0078] Comparative Example 3 and Example 1 differ only in that the modified diatomite is not prepared. Diatomite, river sand, and peat soil are mixed at a volume ratio of 1:1:3 to obtain the cutting substrate.

[0079] Experimental Example 1: After 60 days of cutting in Examples 1-3 and Comparative Examples 1-3, the rooting conditions were counted, and the rooting rate was calculated. The rooting rate (%) = (number of rooted cuttings / total number of cuttings) x 100%, and the results are shown in Table 1. Figure 1

[0080] Figure 1 ​The results show that the rooting rates of the groups of examples 1-3 are significantly better than those of the groups of comparative examples 1-3, the rooting rate of comparative example 1 decreases because the fermented supernatant is replaced by the decoction liquid, indicating that the fermentation of Lactobacillus rhamnosus and Bacillus amyloliquefaciens can promote the rooting of the cuttings, the rooting rate of comparative example 2 decreases because the fermented supernatant is not added, indicating that the rooting agent prepared by the components and method described in the application can effectively promote the rooting of the Xinguan fruit cuttings; the rooting rate of comparative example 3 decreases because the diatomite is not modified and the sulfonated SiO2 is not prepared, the structure of the cutting substrate changes.

[0081] Experimental example 2: After the cuttings of examples 1-3 and comparative examples 1-3 are cut for 60 days, the average number of roots and the average root length of each group are calculated, and the results are shown in Table 2. Figure 2

[0082] Figure 2 The results show that the average number of roots and the average root length of the groups of examples 1-3 are significantly better than those of the groups of comparative examples 1-3, the rooting rate of comparative example 1 decreases because the fermented supernatant is replaced by the decoction liquid, indicating that the fermentation of Lactobacillus rhamnosus and Bacillus amyloliquefaciens can promote the rooting of the cuttings, the rooting rate of comparative example 2 decreases because the fermented supernatant is not added, indicating that the rooting agent prepared by the components and method described in the application can effectively promote the rooting of the Xinguan fruit cuttings; the rooting rate of comparative example 3 decreases because the diatomite is not modified and the sulfonated SiO2 is not prepared, the structure of the cutting substrate changes, and the rooting promoting effect decreases.

[0083] Experimental example 3: After the cuttings of examples 1-3 and comparative examples 1-3 are cut for 90 days, the survival rates of each group are calculated, and the results are shown in Table 3. Figure 3

[0084] Figure 3 The results show that after the cuttings are cut for 90 days, the cuttings of comparative examples 1-3 and comparative examples 1-3 have high survival rates, and the survival rates of examples 1-3 are better than those of comparative examples 1-3, indicating that the Xinguan fruit cutting method of the application has high rooting rate and high survival rate, and can effectively improve the cutting and seedling emergence rate of Xinguan fruit.

[0085] It should be understood by those skilled in the art that the discussion of any of the above examples is only exemplary and is not intended to suggest that the scope of the application is limited to these examples; under the idea of the application, the technical features of the above examples or different examples can also be combined, the steps can be implemented in any order, and there are many other changes of different aspects of the application as described above. In order to be brief, they are not provided in detail.​​

Claims

1. A method for propagating *Sapindus mukorossi* seedlings by rooting cuttings, characterized in that, Includes the following steps: (1) Preparation of cutting substrate: (11) Disperse diatomaceous earth in 5M hydrochloric acid solution, stir, filter, disperse in toluene, add APTES, heat and stir under reflux, filter, and obtain aminated diatomaceous earth. (12) Add CTAB and 1 mol / L NaOH solution to ultrapure water, mix well, stir, add TEOS and MPTMS mixture dropwise, stir, filter, add 0.5 mol / L HCl ethanol solution, reflux, and filter. (13) The product obtained in step (12) is added to H2O2 solution for oxidation, then added to H2SO4 solution, filtered, and sulfonic acid group SiO2 is obtained. (14) Mix the sulfonic acid SiO2 obtained in step (13) and the aminated diatomite obtained in step (11), add it to ultrapure water, stir, filter, wash and dry to obtain modified diatomite. Mix the modified diatomite, river sand and peat soil in a volume ratio of 1:1:3 to obtain the cutting substrate. (2) Preparation of root-promoting agents: (21) After washing and drying licorice, astragalus, platycodon and honeysuckle, pulverize them, add them to deionized water and mix well, sterilize, cool, and inoculate with activated Bacillus amyloliquefaciens and Lactobacillus rhamnosus, wherein the numbers of Bacillus amyloliquefaciens and Lactobacillus rhamnosus are GDMCC NO. 1.3155 and GDMCC NO. 1.2223, respectively; (22) After inoculation in step (21), culture, centrifuge, filter, add indolebutyric acid and mix well to obtain root-promoting agent; (3) Selection and preparation of cuttings; (4) Seedbed setup: Spray the cutting substrate with a 1000-fold carbendazim solution for disinfection and fill it into flowerpots; (5) Pretreatment of cuttings: Immerse the cuttings completely in a 1000-fold dilution of carbendazim, and soak the lower end of the cuttings in a rooting agent; (6) Cuttings; (7) Post-cutting management.

2. The method for propagating *Xanthoceras sorbifolium* by cuttings according to claim 1, characterized in that, In step (11), the mass concentration of the diatomaceous earth in the 5M hydrochloric acid solution is 50 mg / mL; the ratio of diatomaceous earth to APTES is 1 g: 3 mL.

3. The method for propagating *Xanthoceras sorbifolium* by cuttings according to claim 2, characterized in that, In step (12), the ratio of CTAB, 1 mol / L NaOH solution and ultrapure water is 0.5 g:3.5 mL:120 mL; the molar ratio of CTAB, TEOS and MPTMS is 0.11:0.9:0.

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4. The method for propagating *Xanthoceras sorbifolium* by cuttings according to claim 3, characterized in that, In step (14), the ratio of sulfonic acid SiO2, aminated diatomaceous earth and ultrapure water is 1 g:3 g:200 mL.

5. The method for propagating *Xanthoceras sorbifolium* by cuttings according to claim 4, characterized in that, In step (21), the mass ratio of licorice, astragalus, platycodon and honeysuckle is 1:1:3:2.

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