Antibacterial and anti-inflammatory external compound selenium nutrient solution as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of skin external preparations, and particularly relates to an antibacterial and anti-inflammatory external compound selenium nutrient solution and a preparation method and application thereof.The compound selenium nutrient solution is prepared from, by mass, 95-100 parts of magnetized water, 0.01-0.03 part of compound selenium particles and 0.15-0.3 part of a pH regulator, the compound selenium particles comprise sodium selenite, selenium dioxide and selenium disulfide, and the pH regulator comprises sodium selenite, selenium dioxide and selenium disulfide. The mass ratio of the three is (30-50): (5-10): (3-9). According to the invention, through the specific formula design of the compound selenium particles, sodium selenite, selenium dioxide and selenium disulfide are adopted to generate a synergistic effect, so that the curative effect limitation of a single selenium compound is avoided, and the product can cope with various types of body surface microorganisms.

Description

Technical Field

[0001] This invention belongs to the field of topical skin agents, specifically relating to an antibacterial and anti-inflammatory topical selenium nutrient solution, its preparation method, and its application. Background Technology

[0002] As the body's largest barrier organ, the skin is susceptible to bacterial invasion, external trauma, or immune abnormalities, leading to inflammatory responses such as redness, swelling, itching, and ulceration. In severe cases, this can result in the spread of infection or a chronic disease course. With increasing awareness of skin health and daily disinfection, the market demand for topical antibacterial products with disinfection licenses continues to grow, with higher requirements being placed on the products' effectiveness, safety, low irritation, and stability.

[0003] Currently, topical antibacterial products with a disinfection license on the market are mainly divided into two categories: one is chemically synthesized antibacterial agents, such as disinfectants / gels containing benzalkonium chloride, chlorhexidine, iodine, etc. Although they can quickly kill common pathogens on the skin surface, long-term or large-area use can easily lead to dryness and defatting of the skin and mucous membranes, and even cause local allergies and redness; the other is natural antibacterial agents, such as antibacterial care products containing plant essential oils (tea tree oil, honeysuckle extract, etc.) and single mineral elements. Although they have low irritation and good biocompatibility, they generally have the problem of poor stability of active ingredients, which leads to a rapid decline in the effective antibacterial ability after the product is opened. Summary of the Invention

[0004] To address the problems existing in the prior art, this invention provides an antibacterial and anti-inflammatory topical selenium nutrient solution, its preparation method, and its application. Through the specific formulation design of selenium granules, sodium selenite, selenium dioxide, and selenium disulfide are used to produce a synergistic effect, avoiding the limitations of the efficacy of a single selenium compound, and enabling this product to deal with multiple types of microorganisms on the body surface.

[0005] The specific technical solution adopted in this invention is as follows: An antibacterial and anti-inflammatory topical selenium-containing nutrient solution, by weight, comprises 95-100 parts magnetized water, 0.01-0.03 parts selenium granules, and 0.15-0.3 parts pH adjuster; The complex selenium particles consist of sodium selenite, selenium dioxide, and selenium disulfide, with a mass ratio of 30-50:5-10:3-9.

[0006] The magnetized water 17 O-NMR value less than or equal to 60 Hz.

[0007] The pH adjuster is a citrate-sodium citrate buffer pair.

[0008] A method for preparing an antibacterial and anti-inflammatory topical selenium-enriched nutrient solution, the method comprising the following steps: S1. Preparation of complex selenium particles; S2. Add magnetized water to the reaction vessel, then add pH adjuster, stir and dissolve, control the pH to 5.0-6.0, then add selenium granules, continue stirring for 30-45 minutes, let stand for 12-24 hours, and then take the supernatant to obtain selenium nutrient solution.

[0009] The specific preparation method of the selenium-containing particles includes the following steps: S101. Sodium selenite, selenium dioxide, and selenium disulfide are mixed according to the mass ratio, and a 0.5-1% (w / w) aqueous solution of polyvinylpyrrolidone is added. The mixture is ultrasonically dispersed for 20-30 minutes to obtain a mixed selenium suspension. S102. Add the selenium mixed suspension and polyethylene glycol to a ball mill at a mass ratio of 8-12:1 and ball mill at a speed of 200-250 r / min for 2-3 hours. After drying the slurry obtained by ball milling, crush and sieve it to obtain selenium mixed powder. S103. Add the selenium-containing mixed powder to the composite carrier solution and stir at 200-300 r / min for 30-45 min at 35-40℃ to obtain a stable suspension; slowly add 1%-2% sodium alginate solution by mass, and continue stirring for 1.5-2 h after the addition is complete, then add 0.1 mol / L calcium chloride solution and solidify for 60-90 min to obtain coated particles; S104. Centrifuge the coated particles at 8000-10000 r / min for 15-20 min, collect the precipitate, wash it with deionized water 3-5 times, and freeze-dry it for 12-16 h to obtain the selenium-enriched particles.

[0010] The specific preparation method of the composite carrier solution in step S103 is as follows: Hyaluronic acid and chitosan were dissolved in a 0.1 mol / L aqueous acetic acid solution at a mass ratio of 2-3:1 and stirred until completely dissolved to obtain a composite carrier solution with a mass concentration of 1-2%.

[0011] The mass ratio of the selenium mixed powder, composite carrier solution, sodium alginate solution and calcium chloride solution in step S103 is 1:50-80:20-30:20-30.

[0012] An antibacterial and anti-inflammatory topical selenium-enriched nutrient solution is used in an antibacterial solution. The preparation method of the antibacterial solution includes the following steps: Q1. Mix the selenium nutrient solution with glycerin evenly, let it stand, and then filter to obtain the mixed nutrient solution. Q2. Mix the nano silver ion liquid with hydroxyethyl cellulose to obtain a silver ion mixture; Q3. Mix benzalkonium bromide and sodium metabisulfite to obtain benzalkonium bromide solution; Q4. Mix the mixed nutrient solution, silver ion mixed solution, benzalkonium bromide solution and hydroxypropyl betacyclodextrin evenly to obtain the finished antibacterial solution.

[0013] The finished antibacterial solution, by weight, comprises 70-90 parts of selenium nutrient solution, 1.5-2 parts of glycerol, 0.01-0.03 parts of nano silver ion solution with a concentration of 1000ppm, 0.1-0.2 parts of hydroxyethyl cellulose, 0.1-0.3 parts of benzalkonium bromide, 0.1-0.2 parts of sodium metabisulfite, and 0.2-0.5 parts of hydroxypropyl betacyclodextrin.

[0014] The beneficial effects of this invention are: 1. This invention utilizes a specific formulation design for selenium-containing granules, employing sodium selenite, selenium dioxide, and selenium disulfide to achieve a synergistic effect. Selenium can reduce skin inflammation by inhibiting the release of inflammatory mediators, while sodium selenite can rapidly release... By disrupting the phospholipid bilayer of the bacterial cell membrane, intracellular substances leak out, while simultaneously inhibiting the activity of key bacterial enzymes and blocking energy metabolism; selenium dioxide is slowly released. By oxidizing reducing substances within bacteria, selenium sulfide disrupts the redox balance and inhibits bacterial proliferation. It also easily penetrates the bacterial cell wall, binding to the sulfhydryl groups of cell wall proteins, causing protein denaturation, and simultaneously inhibits ergosterol synthesis in fungi. This synergistic antibacterial system overcomes the narrow antibacterial spectrum problem of single selenium compounds, enabling this product to target a wide range of surface microorganisms.

[0015] 2. This invention addresses the short duration of efficacy of existing topical skin treatments through carrier encapsulation. Firstly, the composite carrier of hyaluronic acid and chitosan possesses both biocompatibility and adhesiveness, forming a thin film on the skin surface to slow the release rate of selenium. The solidification reaction of sodium alginate and calcium chloride further forms a stable encapsulation structure, allowing the active ingredients to act on the skin at a slow and continuous rate, preventing a rapid drop in concentration and significantly extending the duration of efficacy.

[0016] 3. In this invention, magnetized water is used as a solvent. Magnetized water has smaller molecular clusters and stronger penetration ability, which can carry active ingredients such as selenium particles and silver ions to quickly penetrate to the skin surface and reach the site of inflammation or bacterial colonization, thus avoiding the poor effect caused by active ingredients remaining on the skin surface.

[0017] 4. The antibacterial solution in this invention forms a multi-layered antibacterial system through selenium nutrient solution, nano silver ion solution, and benzalkonium bromide. Their mechanisms of action are synergistic. Specifically, after benzalkonium bromide disrupts the cell membrane, selenium ions and Ag... + It is easier to enter the cell, which improves the antibacterial and anti-inflammatory effects of antibacterial agents while significantly reducing the amount of a single ingredient used and reducing the risk of drug resistance. Detailed Implementation

[0018] The present invention will be further described below with reference to specific embodiments: I. Specific Implementation Methods Example 1 S101. Sodium selenite, selenium dioxide, and selenium disulfide are mixed in a mass ratio of 40:8:5 and added to a 1% (w / w) aqueous solution of polyvinylpyrrolidone. The mixture is then ultrasonically dispersed for 30 min to obtain a complex selenium suspension. S102. Add the selenium mixed suspension and polyethylene glycol to a ball mill at a mass ratio of 10:1 and ball mill at 250 r / min for 2 h. After drying the slurry obtained by ball milling, crush and sieve it to obtain selenium mixed powder. S103. Hyaluronic acid and chitosan were dissolved in 0.1 mol / L acetic acid aqueous solution at a mass ratio of 2:1 and stirred until completely dissolved to obtain a composite carrier solution with a mass concentration of 2%. The selenium mixed powder was added to the composite carrier solution and stirred at 300 r / min for 45 min at 35℃ to obtain a stable suspension. A sodium alginate solution with a mass concentration of 1% was slowly added dropwise. After the addition was completed, stirring was continued for 1.5 h. Then, a calcium chloride solution with a mass concentration of 0.1 mol / L was added dropwise and solidified for 60 min to obtain coated particles. The mass ratio of selenium mixed powder, composite carrier solution, sodium alginate solution and calcium chloride solution is 1:60:25:25; S104. The coated particles were centrifuged at 10000 r / min for 15 min, the precipitate was collected, washed 5 times with deionized water, and freeze-dried for 12 h to obtain the selenium-enriched particles. S2. Add magnetized water to the reaction vessel, then add citric acid-sodium citrate buffer and stir to dissolve, control the pH to 5.5, then add selenium granules, continue stirring for 45 minutes, let stand for 18 hours, and take the supernatant to obtain selenium nutrient solution.

[0019] Example 2 S101. Sodium selenite, selenium dioxide, and selenium disulfide are mixed in a mass ratio of 30:5:3 and added to a 1% (w / w) aqueous solution of polyvinylpyrrolidone. The mixture is then ultrasonically dispersed for 30 min to obtain a complex selenium suspension. S102. Add the selenium mixed suspension and polyethylene glycol to a ball mill at a mass ratio of 8:1 and ball mill at 200 r / min for 2 hours. After drying the slurry obtained by ball milling, crush and sieve it to obtain selenium mixed powder. S103. Hyaluronic acid and chitosan were dissolved in 0.1 mol / L acetic acid aqueous solution at a mass ratio of 3:1 and stirred until completely dissolved to obtain a 2% (w / w) composite carrier solution. The selenium-containing mixed powder was added to the composite carrier solution and stirred at 300 r / min for 45 min at 35℃ to obtain a stable suspension. A 1% (w / w) sodium alginate solution was slowly added dropwise. After the addition was completed, stirring was continued for 1.5 h. Then, a 0.1 mol / L calcium chloride solution was added dropwise and solidified for 60 min to obtain coated particles. The mass ratio of selenium mixed powder, composite carrier solution, sodium alginate solution and calcium chloride solution is 1:50:20:20; S104. The coated particles were centrifuged at 10000 r / min for 15 min, the precipitate was collected, washed 5 times with deionized water, and freeze-dried for 12 h to obtain the selenium-enriched particles. S2. Add magnetized water to the reaction vessel, then add citric acid-sodium citrate buffer and stir to dissolve, control the pH to 6.0, then add selenium granules, continue stirring for 45 minutes, let stand for 18 hours, and take the supernatant to obtain selenium nutrient solution.

[0020] Example 3 S101. Sodium selenite, selenium dioxide, and selenium disulfide are mixed in a mass ratio of 50:10:9 and added to a 1% (w / w) aqueous solution of polyvinylpyrrolidone. The mixture is then ultrasonically dispersed for 30 min to obtain a complex selenium suspension. S102. Add the selenium mixed suspension and polyethylene glycol to a ball mill at a mass ratio of 12:1 and ball mill at 250 r / min for 3 h. After drying the slurry obtained by ball milling, crush and sieve it to obtain selenium mixed powder. S103. Hyaluronic acid and chitosan are dissolved in 0.1 mol / L acetic acid aqueous solution at a mass ratio of 2:1 and stirred until completely dissolved to obtain a 1% mass concentration composite carrier solution. The selenium mixed powder is added to the composite carrier solution and stirred at 200 r / min for 45 min at 40℃ to obtain a stable suspension. A 2% mass concentration sodium alginate solution is slowly added dropwise. After the addition is complete, stirring is continued for 2 h. Then, a 0.1 mol / L calcium chloride solution is added dropwise and solidified for 60 min to obtain coated particles. The mass ratio of selenium mixed powder, composite carrier solution, sodium alginate solution and calcium chloride solution is 1:80:30:30; S104. The coated particles were centrifuged at 8000 r / min for 20 min, the precipitate was collected, washed 5 times with deionized water, and freeze-dried for 16 h to obtain the selenium-enriched particles. S2. Add magnetized water to the reaction vessel, then add citric acid-sodium citrate buffer and stir to dissolve, control the pH to 5.0, then add selenium granules, continue stirring for 45 minutes, let stand for 24 hours, and take the supernatant to obtain selenium nutrient solution.

[0021] Example 4 S101. Sodium selenite, selenium dioxide, and selenium disulfide are mixed in a mass ratio of 42:6:6 and added to a 0.5% polyvinylpyrrolidone aqueous solution. The mixture is ultrasonically dispersed for 20 minutes to obtain a complex selenium mixed suspension. S102. Add the selenium mixed suspension and polyethylene glycol to a ball mill at a mass ratio of 10:1 and ball mill at 250 r / min for 2 h. After drying the slurry obtained by ball milling, crush and sieve it to obtain selenium mixed powder. S103. Hyaluronic acid and chitosan are dissolved in 0.1 mol / L acetic acid aqueous solution at a mass ratio of 2:1 and stirred until completely dissolved to obtain a 1% mass concentration composite carrier solution. The selenium mixed powder is added to the composite carrier solution and stirred at 300 r / min for 30 min at 40℃ to obtain a stable suspension. A 1% mass concentration sodium alginate solution is slowly added dropwise. After the addition is complete, stirring is continued for 2 h. Then, a 0.1 mol / L calcium chloride solution is added dropwise and solidified for 90 min to obtain coated particles. The mass ratio of selenium mixed powder, composite carrier solution, sodium alginate solution and calcium chloride solution is 1:65:24:24; S104. The coated particles were centrifuged at 8000 r / min for 20 min, the precipitate was collected, washed 5 times with deionized water, and freeze-dried for 16 h to obtain the selenium-enriched particles. S2. Add magnetized water to the reaction vessel, then add citric acid-sodium citrate buffer and stir to dissolve, control the pH to 5.5, then add selenium granules, continue stirring for 45 minutes, let stand for 24 hours, and then take the supernatant to obtain selenium nutrient solution.

[0022] Example 5 S101. Sodium selenite, selenium dioxide, and selenium disulfide are mixed in a mass ratio of 30:10:5 and added to a 1% (w / w) aqueous solution of polyvinylpyrrolidone. The mixture is then ultrasonically dispersed for 30 min to obtain a complex selenium suspension. S102. Add the selenium mixed suspension and polyethylene glycol to a ball mill at a mass ratio of 10:1 and ball mill at 250 r / min for 2 h. After drying the slurry obtained by ball milling, crush and sieve it to obtain selenium mixed powder. S103. Hyaluronic acid and chitosan were dissolved in 0.1 mol / L acetic acid aqueous solution at a mass ratio of 2:1 and stirred until completely dissolved to obtain a 1% mass concentration composite carrier solution. The selenium mixed powder was added to the composite carrier solution and stirred at 300 r / min for 30 min at 35℃ to obtain a stable suspension. A 1% mass concentration sodium alginate solution was slowly added dropwise. After the addition was completed, stirring was continued for 2 h. Then, a 0.1 mol / L calcium chloride solution was added dropwise and solidified for 90 min to obtain coated particles. The mass ratio of selenium mixed powder, composite carrier solution, sodium alginate solution and calcium chloride solution is 1:60:25:25; S104. The coated particles were centrifuged at 8000 r / min for 20 min, the precipitate was collected, washed 5 times with deionized water, and freeze-dried for 12 h to obtain the selenium-enriched particles. S2. Add magnetized water to the reaction vessel, then add citric acid-sodium citrate buffer and stir to dissolve, control the pH to 5.5, then add selenium granules, continue stirring for 45 minutes, let stand for 24 hours, and then take the supernatant to obtain selenium nutrient solution.

[0023] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that the selenium-containing compound in Comparative Example 1 was not coated. The preparation method in Comparative Example 1 is as follows: Magnetized water was added to the reaction vessel, and then citric acid-sodium citrate buffer was added and stirred to dissolve the mixture. The pH was controlled at 5.5. Sodium selenite, selenium dioxide, and selenium disulfide were then mixed and added to the solution at a mass ratio of 40:8:5. The mixture was stirred for 45 minutes and allowed to stand for 18 hours. The supernatant was then collected to obtain the selenium nutrient solution.

[0024] II. Performance Testing The selenium-enriched nutrient solutions obtained in Examples 1-5 and Comparative Example 1 were used to prepare antibacterial solutions. The preparation method of the antibacterial solutions is as follows: Q1. Prepare the raw materials according to the following mass ratios: 70-90 parts selenium nutrient solution, 1.5-2 parts glycerin, 0.01-0.03 parts nano silver ion solution with a concentration of 1000ppm, 0.1-0.2 parts hydroxyethyl cellulose, 0.1-0.3 parts benzalkonium bromide, 0.1-0.2 parts sodium metabisulfite, and 0.2-0.5 parts hydroxypropyl betacyclodextrin. Q2. Mix the selenium nutrient solution with glycerin evenly, let it stand, and then filter to obtain the mixed nutrient solution. Q3. Mix the nano silver ion liquid with hydroxyethyl cellulose to obtain a silver ion mixture; Q4. Mix benzalkonium bromide and sodium metabisulfite to obtain benzalkonium bromide solution; Q5. Mix the mixed nutrient solution, silver ion mixture, benzalkonium bromide solution and hydroxypropyl betacyclodextrin evenly to obtain the finished antibacterial solution.

[0025] The prepared antibacterial solution was tested for its bactericidal rate and antibacterial duration against common bacteria. The method for testing the antibacterial duration was as follows: the antibacterial solution was applied to a simulated skin carrier, and the antibacterial solution was collected from the simulated skin carrier after 0.5h, 2h, 8h, 12h, and 24h. The collected antibacterial solution was then used to test the bactericidal rate of bacteria. The contact time of the antibacterial solution during the bactericidal rate test was 5min. The specific test results are shown in Tables 1-4.

[0026] Table 1 Table 2 Table 3 Table 4 As can be seen from Table 1, the antibacterial solution prepared using the selenium nutrient solution of the present invention has a good bactericidal effect against common bacteria. Within 0.5 hours, the bactericidal rate of Examples 1-5 and Comparative Example 1 against various bacterial species remained above 99.0%. This is because the effective components in the antibacterial solution are at a high concentration in the initial stage, which can quickly act on the cell membrane of bacteria, destroy the bacterial structure, and achieve efficient bactericidal effect.

[0027] However, in Comparative Example 1, the antibacterial solution did not coat the selenium particles, resulting in the release and loss of a large amount of selenium compounds within 8 hours, reducing the sterilization rate to 95.80-96.31%, further decreasing to 93.00-93.5% after 12 hours, and plummeting to 86.80-87.3% after 24 hours. Due to the lack of coating, the effective ingredients were basically released within 24 hours, and the solution lacked continuous penetration and antioxidant protection, making it easy for bacteria to re-proliferate.

[0028] The antibacterial liquid in this invention still achieves a sterilization rate of over 95% after 24 hours. This is because it uses a composite carrier for encapsulation, which can slowly release selenium compounds. At the same time, hydroxypropyl betacyclodextrin stabilizes the nano-silver ions, preventing the rapid loss of effective ingredients.

[0029] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A topical selenium-enriched nutrient solution with antibacterial and anti-inflammatory properties, characterized in that, By weight, it includes 95-100 parts magnetized water, 0.01-0.03 parts selenium granules, and 0.15-0.3 parts pH adjuster; The complex selenium particles consist of sodium selenite, selenium dioxide, and selenium disulfide, with a mass ratio of 30-50:5-10:3-9.

2. The antibacterial and anti-inflammatory external selenium-enriched nutrient solution according to claim 1, characterized in that, The magnetized water 17 O-NMR value less than or equal to 60 Hz.

3. The antibacterial and anti-inflammatory external selenium-enriched nutrient solution according to claim 1, characterized in that, The pH adjuster is a citrate-sodium citrate buffer pair, by mass fraction.

4. A method for preparing an antibacterial and anti-inflammatory topical selenium-enriched nutrient solution, used to prepare the antibacterial and anti-inflammatory topical selenium-enriched nutrient solution as described in claim 1, characterized in that, The preparation method includes the following steps: S1. Preparation of complex selenium particles; S2. Add magnetized water to the reaction vessel, then add pH adjuster, stir and dissolve, control the pH to 5.0-6.0, then add selenium granules, continue stirring for 30-45 minutes, let stand for 12-24 hours, and then take the supernatant to obtain selenium nutrient solution.

5. The method for preparing an antibacterial and anti-inflammatory topical selenium-enriched nutrient solution according to claim 4, characterized in that, The specific preparation method of the selenium-containing particles includes the following steps: S101. Sodium selenite, selenium dioxide, and selenium disulfide are mixed according to the mass ratio, and a 0.5-1% (w / w) aqueous solution of polyvinylpyrrolidone is added. The mixture is ultrasonically dispersed for 20-30 minutes to obtain a mixed selenium suspension. S102. Add the selenium mixed suspension and polyethylene glycol to a ball mill at a mass ratio of 8-12:1 and ball mill at a speed of 200-250 r / min for 2-3 hours. After drying the slurry obtained by ball milling, crush and sieve it to obtain selenium mixed powder. S103. Add the selenium-containing mixed powder to the composite carrier solution and stir at 200-300 r / min for 30-45 min at 35-40℃ to obtain a stable suspension; slowly add 1%-2% sodium alginate solution by mass, and continue stirring for 1.5-2 h after the addition is complete, then add 0.1 mol / L calcium chloride solution and solidify for 60-90 min to obtain coated particles; S104. Centrifuge the coated particles at 8000-10000 r / min for 15-20 min, collect the precipitate, wash it with deionized water 3-5 times, and freeze-dry it for 12-16 h to obtain the selenium-enriched particles.

6. The method for preparing an antibacterial and anti-inflammatory topical selenium-enriched nutrient solution according to claim 5, characterized in that, The specific preparation method of the composite carrier solution in step S103 is as follows: Hyaluronic acid and chitosan were dissolved in a 0.1 mol / L aqueous acetic acid solution at a mass ratio of 2-3:1 and stirred until completely dissolved to obtain a composite carrier solution with a mass concentration of 1-2%.

7. The method for preparing an antibacterial and anti-inflammatory topical selenium-enriched nutrient solution according to claim 5, characterized in that, The mass ratio of the selenium mixed powder, composite carrier solution, sodium alginate solution and calcium chloride solution in step S103 is 1:50-80:20-30:20-30.

8. The application of an antibacterial and anti-inflammatory topical selenium-enriched nutrient solution in an antibacterial solution, wherein the antibacterial solution contains the antibacterial and anti-inflammatory topical selenium-enriched nutrient solution as described in claim 1, characterized in that... The method for preparing the antibacterial solution includes the following steps: Q1. Mix the selenium nutrient solution with glycerin evenly, let it stand, and then filter to obtain the mixed nutrient solution. Q2. Mix the nano silver ion liquid with hydroxyethyl cellulose to obtain a silver ion mixture; Q3. Mix benzalkonium bromide and sodium metabisulfite to obtain benzalkonium bromide solution; Q4. Mix the mixed nutrient solution, silver ion mixed solution, benzalkonium bromide solution and hydroxypropyl betacyclodextrin evenly to obtain the finished antibacterial solution.

9. The application of the antibacterial and anti-inflammatory topical selenium nutrient solution according to claim 8 in an antibacterial solution, characterized in that, The finished antibacterial solution, by weight, comprises 70-90 parts of selenium nutrient solution, 1.5-2 parts of glycerol, 0.01-0.03 parts of nano silver ion solution with a concentration of 1000ppm, 0.1-0.2 parts of hydroxyethyl cellulose, 0.1-0.3 parts of benzalkonium bromide, 0.1-0.2 parts of sodium metabisulfite, and 0.2-0.5 parts of hydroxypropyl betacyclodextrin.

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