Composition comprising plant extract for reducing skin injury caused by ultraviolet rays
By using a specific ratio of plant extracts, this product solves the problems of photosensitivity and UV exposure caused by existing sunscreens, achieving natural and effective skin protection and reducing UV damage and aging.
Patent Information
- Application Number
- CN202511118061.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2017-05-08
- Filing Date
- 2018-04-03
- Publication Date
- 2025-11-04
AI Technical Summary
Existing sunscreens may cause photosensitivity reactions during use, and many people fail to effectively avoid UV exposure, leading to skin aging and damage.
A specific ratio of plant extracts, such as a mixture of spinach extract with red wine extract, blueberry extract, or citrus extract, is used as a natural sunscreen composition to reduce UV damage to the skin.
It significantly reduces fibroblast death caused by ultraviolet radiation, enhances the skin's UV barrier function, slows down skin aging, and reduces the incidence of skin cancer.
Smart Images
Figure CN120884639A_ABST
Abstract
Description
[0001] Cross-references to related applications
[0002] This application is a divisional application of Chinese national patent application No. 201880021268.X, filed on April 3, 2018, entitled "A plant extract composition for reducing ultraviolet damage".
[0003] This application claims priority to U.S. Patent Application No. 62 / 480,860, filed in the United States on April 3, 2017, and U.S. Patent Application No. 62 / 503,185, filed in the United States on May 8, 2017, the entire contents of which are incorporated herein by reference. Technical Field
[0004] This application relates to a composition comprising plant extracts and its use, and more particularly to a composition for reducing skin damage caused by ultraviolet radiation and its use. Background Technology
[0005] The sun is the primary source of ultraviolet (UV) radiation for most people. UV radiation can be further divided into UVA (315–400 nm), UVB (280–315 nm), and UVC (100–280 nm). UVC is absorbed by the atmosphere and does not reach the Earth's surface. UVB is the most damaging form of UV radiation to living organisms, yet most of it is absorbed by the atmosphere. Longer wavelength UVA is the most intense form of UV radiation that reaches Earth and can penetrate deep into body tissues. Studies have found that exposure to UV radiation increases the likelihood of accelerated skin aging and skin cancer, and may also reduce an individual's resistance to infectious diseases.
[0006] Skin is the largest body surface area in adults exceeding 1.5 square meters. 2 The skin is a large organ that provides the first line of protection against ultraviolet (UV) radiation from the sun. The skin comprises the epidermis (including the outermost stratum corneum), the dermis, and the subcutaneous tissue. The epidermis is the outermost layer of the skin and is constantly renewing itself. Between the epidermis and dermis lie continuously dividing cells (such as fibroblasts, keratinocytes, and melanocytes), whose activity is highly sensitive to UV radiation. The dermis contains collagen and elastin, which give the skin its elasticity and support. The skin prevents damage from UV radiation to the tissues below the epidermis by increasing melanin production and promoting cell proliferation to thicken the outer layer (stratum corneum). For example, when exposed to high levels of UV radiation (primarily UVA), collagen fibers and elastin are damaged, resulting in reduced skin elasticity, wrinkle formation, and an aged appearance.
[0007] The most common way to reduce the damage caused by UV radiation is to avoid sun exposure and to use sunscreens. To get adequate protection, sunscreens should be applied liberally to the skin and the thickness should be ensured and allowed to dry for about 15 minutes before going outside. In addition, the thickness should be maintained by reapplying the sunscreen at intervals. Most people do not get good protection because they do not use sunscreens according to the foregoing method. In some cases, the chemicals contained in sunscreens can cause photosensitization, so that the combination of these chemicals with UV radiation has an adverse effect on the skin, such as skin rash or more serious sunburn. SUMMARY
[0008] In view of the foregoing, the present application provides a composition for reducing skin damage caused by UV radiation comprising plant extracts, the composition consisting of two plant extracts in a ratio of 1:1, the plant extracts including spinach extract and one selected from the group consisting of red wine extract, blueberry extract, and citrus extract. The composition for reducing skin damage caused by UV radiation comprising plant extracts according to the present application is natural and effective in protecting the skin from UV damage to slow down skin aging or reduce the incidence of skin cancer.
[0009] In an embodiment of the present application, the composition consists of spinach extract and red wine extract.
[0010] In an embodiment of the present application, the concentration of the spinach extract in the composition is 0.25 mg / ml, and the concentration of the red wine extract is 0.25 mg / ml.
[0011] In an embodiment of the present application, the composition consists of spinach extract and blueberry extract.
[0012] In an embodiment of the present application, the concentration of the spinach extract in the composition is 0.5 mg / ml, and the concentration of the blueberry extract is 0.5 mg / ml.
[0013] In an embodiment of the present application, the composition consists of citrus extract and spinach extract.
[0014] In an embodiment of the present application, the concentration of the citrus extract in the composition is 0.25 mg / ml, and the concentration of the spinach extract is 0.25 mg / ml.
[0015] The present application provides a composition for reducing skin damage caused by UV radiation comprising plant extracts, the composition consisting of citrus extract and red wine extract in a ratio of 1:1.
[0016] In an embodiment of the present application, the concentration of the citrus extract in the composition is 0.25 mg / ml, and the concentration of the red wine extract is 0.25 mg / ml.
[0017] In one embodiment of the present application, the above composition further comprises a pharmaceutically acceptable carrier.
[0018] In one embodiment of the present application, the above composition is in the form of a solution, a powder, a capsule, or a tablet.
[0019] It is another object of the present application to provide the use of any of the above compositions for reducing the damage to skin fibroblasts caused by ultraviolet light, or the use of any of the above compositions in the preparation of a pharmaceutical composition for reducing the damage to skin caused by ultraviolet light.
[0020] In one embodiment of the present application, the above ultraviolet light is ultraviolet A light.
[0021] The composition of the present application significantly inhibits the death of skin fibroblasts caused by ultraviolet light by mixing specific plant extracts in specific proportions. The normal proliferation of skin fibroblasts is beneficial to maintaining the thickness of the epidermis and the ultraviolet light barrier function, and can also replenish the components in the extracellular matrix of the skin and maintain the renewal of the dermis. In other words, the administration of an effective amount of the composition of the present application to an individual significantly reduces the damage to the skin caused by ultraviolet light in the individual. Therefore, the composition of the present application can be used in the preparation of a pharmaceutical composition for protecting the skin against damage caused by ultraviolet light. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 The effects of different compositions on the cell proliferation rate of skin fibroblasts under ultraviolet light irradiation in one embodiment of the present application are shown. DETAILED DESCRIPTION
[0023] The embodiments of the present application will be further described in conjunction with the accompanying drawings, and the following enumerated examples are provided to illustrate the features and applications of the present application, but not to limit the scope of the present application. Those skilled in the art can make several improvements and refinements without departing from the spirit and scope of the present application, and therefore the scope of protection of the present application should be determined by the contents of the claims of the present application.
[0024] DEFINITIONS
[0025] The numerical values used herein are approximate values, and all experimental data are expressed within a range of 20%, preferably within a range of 10%, and most preferably within a range of 5%.
[0026] As used herein, the term "pharmaceutically acceptable carrier" refers to one or more solid or liquid medium which is not toxic to mammals and does not affect the biological activity of the active ingredients in the composition.
[0027] The present invention provides a composition for reducing skin damage caused by ultraviolet rays, which comprises a plurality of plant extracts. The composition is prepared by mixing extracts of spinach, black tea, green tea, four seasons spring tea, red wine, blueberry, grape seed, citrus, or green coffee beans. The composition is disclosed in the following examples to substantially inhibit skin fibroblast death caused by ultraviolet A irradiation.
[0028] Materials and Methods
[0029] Materials
[0030] Eagle's minimum essential medium (MEM medium) containing Earle's balanced salt solution, fetal bovine serum (FBS), penicillin / streptomycin, and phosphate buffered saline (PBS solution) were purchased from Gibco. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) for cell survival assay was purchased from AMERSCO. Dimethyl sulfoxide (DMSO) was purchased from Echo chemical. Apple polyphenols were purchased from Jiwang Co., Ltd.
[0031] Cell Culture
[0032] The following examples were performed using human skin fibroblast CCD-966SK (ATCC CRL-1881). Human skin fibroblasts were cultured in MEM medium supplemented with 10% FBS and 1% penicillin / streptomycin at 37°C in 5% carbon dioxide, hereinafter referred to as cell culture medium.
[0033] MTT Assay
[0034] Cell survival or proliferation was determined by MTT assay. Briefly, MTT solution (4 mg / ml MTT dissolved in PBS solution) was added to cells in a 96-well plate at 15 μl / well and reacted for 4 hours at room temperature. After removing the reaction solution, DMSO was added to cells at 50 μl / well and shaken for 10 minutes to dissolve the formazan produced in cells. The absorbance of the solution was measured at 570 nm using a microplate reader. (formazan) crystals. Finally, the absorbance of the cell mixture at 570 nm (O.D. 570) was measured using an ELISA reader (enzyme-linked immunosorbent assay reader; BioTek). Statistically significant differences were analyzed by Student's t-test using Excel software.
[0035] Example 1 Preparation of plant extracts
[0036] Example 1-1 Spinach extract
[0037] The spinach extract was obtained by extraction of Spinacia oleracea and was purchased from Hongxiang Agricultural Processing Factory.
[0038] Example 1-2 Black tea extract
[0039] This example illustrates a method for preparing a black tea extract. First, the fermented leaves of Camellia sinensis were washed and dried, and then coarsely ground using a grinder. Next, the black tea coarsely ground material was extracted using water as the solvent. The solvent was uniformly mixed with the black tea coarsely ground material at a liquid-to-solid ratio of 5-20: 1-5, and the extraction temperature was 50-100 °C, preferably 75-95 °C. The extraction time was about 0.5-3 hours. After the black tea extract obtained from the extraction step was cooled to room temperature, it was filtered through a 400 mesh filter screen to remove residual solid material. The filtered black tea extract can be further concentrated under reduced pressure at 45-70 °C to obtain a concentrated product.
[0040] Example 1-3 Green tea extract
[0041] This example illustrates a method for preparing a green tea extract. First, the unfermented leaves of Camellia sinensis were washed and dried, and then coarsely ground using a grinder. Next, the green tea coarsely ground material was extracted using water as the solvent. The solvent was uniformly mixed with the green tea coarsely ground material at a liquid-to-solid ratio of 5-20: 1-5, and the extraction temperature was 50-100 °C, preferably 75-95 °C. The extraction time was about 0.5-3 hours. After the green tea extract obtained from the extraction step was cooled to room temperature, it was filtered through a 400 mesh filter screen to remove residual solid material. The filtered green tea extract can be further concentrated under reduced pressure at 45-70 °C to obtain a concentrated product.
[0042] Example 1-4 Pu'er tea extract
[0043] A Puerh tea extract is obtained by extraction of post-fermented leaves of Camellia sinensis, which is commercially available from Nanjing Zelang Biotechnology Co., Ltd.
[0044] Example 1-5 Four Seasons Spring tea extract
[0045] This example illustrates a method for preparing a Four Seasons Spring tea extract. First, the leaves of Four Seasons Spring tea plant are washed and dried, and then coarsely ground by a grinder. Next, the coarsely ground Four Seasons Spring tea is extracted with water as a solvent, which is uniformly mixed with the coarsely ground Four Seasons Spring tea at a liquid-to-solid ratio of 5-20: 1-5, and the extraction temperature is 50-100 °C, preferably 75-95 °C. The extraction time is about 0.5-3 hours. After the Four Seasons Spring tea extract obtained from the extraction step is cooled to room temperature, it is filtered through a 400-mesh filter screen to remove residual solid materials. The filtered Four Seasons Spring tea extract can be further concentrated under reduced pressure at 45-70 °C to obtain a concentrated product.
[0046] Example 1-6 Red wine extract
[0047] A red wine extract is obtained by extraction of red wine, which is commercially available from Shanghai Boyutang Biotechnology Co., Ltd.
[0048] Example 1-7 Green coffee bean extract
[0049] A green coffee bean extract is obtained by extraction of seeds of Coffea spp. that are not roasted, which is commercially available from ARJUNA NATURAL EXTRACTS Pvt. Ltd. (India).
[0050] Example 1-8 Blueberry extract
[0051] A blueberry extract is obtained by extraction of fruits of Vaccinium Cyanococcus, which is commercially available from Yuanhong Herbal Research Co., Ltd.
[0052] Example 1-9 Citrus extract
[0053] A citrus extract is obtained by extraction of fruits of Citrus reticulata, which is commercially available from Ruttons Trading Co. Ltd.
[0054] Example 1-10 Grape seed extract
[0055] Grape seed extract is obtained from the extraction of seeds of plants of the genus Vitis spp. and is commercially available from Honson Biotech Co., Ltd.
[0056] Example 1-11 Rosemary extract
[0057] Rosemary extract is obtained from the extraction of Rosmarinus officinalis and is commercially available from Jiajingbaocao Co., Ltd.
[0058] Example 2
[0059] Plant extract-containing compositions inhibit skin fibroblast death caused by UV-A irradiation
[0060] To test the effect of the compositions of the present application in protecting skin against UV irradiation, cell proliferation rates of human skin fibroblasts CCD-966SK treated with plant extracts or combinations thereof after UV-A irradiation were evaluated using cell viability assay (MTT assay). Briefly, CCD-966SK cells were seeded at 5 x 10 3 cells / well in 96-well plates containing 200 μl of cell culture medium and incubated at 37°C for 24 hours. The cell culture medium was then removed and the cells were subjected to 15 J / cm 2 UV-A irradiation for 1 hour, which dose of radiation caused 50% of the cells to die. Thereafter, the plant extracts listed in Table 1 or the compositions containing the plant extracts were added to the cells together with the cell culture medium and incubated at 37°C for 24 hours. Another set of cells that were subjected to UV-A irradiation but treated with cell culture medium without plant extracts was set up as a negative control, and another set of cells that were not subjected to UV-A irradiation but treated with cell culture medium without plant extracts was set up as a blank control. Finally, MTT assay was performed and the cell proliferation rates of the cells in each group were calculated. The cell proliferation rate was calculated by the following formula:
[0061] Cell proliferation rate = O.D.570 of each group / O.D.570 of the negative control x 100%
[0062] Table 1
[0063]
[0064]
[0065]
[0066] Table 1 shows the cell proliferation rates of skin fibroblasts after different treatments; Figure 1is a histogram of the values listed in Table 1. According to Table 1, the cell proliferation rate of the blank control group relative to the negative control group was 237%, indicating that the UV-A irradiation caused a large number of skin fibroblasts to die. According to Table 1 and Figure 1 , the individual treatment of the spinach extract (0.25 mg / ml) or the citrus extract (0.5 mg / ml) caused the cell proliferation rate to increase to 138% and 142%, respectively, relative to the negative control group. In addition, the individual treatment of the black tea extract, the green tea extract, the Pu'er tea extract, the Lapsang Souchong tea extract, the green coffee bean extract, the blueberry extract, or the grape seed extract caused the cell proliferation rate to vary between about 95% and 140%. The individual treatment of the red wine extract caused the cell proliferation rate to be about 170%.
[0067] Notably, the combination of the spinach extract and the black tea extract, the blueberry extract, or the grape seed extract significantly increased the cell proliferation rate of the skin fibroblasts by 180%, 220%, and 168%, respectively; the combination of the spinach extract and the red wine extract increased the cell proliferation rate to about 250%. Similarly, the combination of the citrus extract at a concentration equal to or lower than 0.5 mg / ml and the black tea extract, the green tea extract, the Pu'er tea extract, the Lapsang Souchong tea extract, the green coffee bean extract, the spinach extract, or the grape seed extract significantly increased the cell proliferation rate to between 142% and 204%; the combination of the citrus extract and the red wine extract increased the cell proliferation rate to about 209%. The compositions having the above-mentioned specific combinations unexpectedly exhibited higher protection against UV-A than the simple addition of the individual components.
[0068] In summary, the compositions of the present application inhibit the death of skin fibroblasts caused by UV irradiation by mixing specific plant extracts. The normal proliferation of skin fibroblasts is conducive to maintaining the thickness of the epidermis and the UV barrier function, and can also replenish the components in the extracellular matrix of the skin and maintain the renewal of the dermis. Therefore, the compositions of the present application can be used in the preparation of a composition for reducing skin damage caused by UV light, together with a pharmaceutically acceptable carrier. The dosage form of the composition can be, but is not limited to, a solution, a powder, a capsule, or a tablet.
Claims
1. A composition comprising plant extracts for reducing UV-induced skin damage, characterized in that, The composition comprises two plant extracts in a 1:1 ratio, the plant extracts including spinach extract and one of red wine extract, blueberry extract or citrus extract.
2. The composition according to claim 1, characterized in that, The composition consists of spinach extract and red wine extract.
3. The composition according to claim 2, characterized in that, The concentration of spinach extract in the composition is 0.25 mg / ml, and the concentration of red wine extract is 0.25 mg / ml.
4. The composition according to claim 1, characterized in that, The composition consists of spinach extract and blueberry extract.
5. The composition according to claim 4, characterized in that, The concentration of spinach extract in the composition is 0.5 mg / ml, and the concentration of blueberry extract is 0.5 mg / ml.
6. The composition according to claim 1, characterized in that, The composition consists of citrus extract and spinach extract.
7. The composition according to claim 6, characterized in that, The concentration of citrus extract in the composition is 0.25 mg / ml, and the concentration of spinach extract is 0.25 mg / ml.
8. A composition comprising plant extracts for reducing UV-induced skin damage, characterized in that, The composition consists of a 1:1 ratio of citrus extract and red wine extract.
9. The composition according to claim 8, characterized in that, The concentration of citrus extract in the composition is 0.25 mg / ml, and the concentration of red wine extract is 0.25 mg / ml.
10. The composition of claim 1 or 8 further comprises a pharmaceutically acceptable carrier.