A transparent protein electrolyte sports drink and a method for preparing the same

Abstract

The present application relates to the technical field of food, in particular to a transparent protein electrolyte sports drink, comprising the following raw materials: nanofiltration filtrate, sugar, decolorized and deacidified apple concentrate juice, whey protein, acidity regulator, blueberry extract, needle cherry concentrate powder, natural lemon essence and water; a preparation method comprising the following steps: mixing the nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain a liquid A; mixing the decolorized and deacidified apple concentrate juice, acidity regulator, blueberry extract, needle cherry concentrate powder and water to obtain a liquid B; mixing the liquid A after cooling and the liquid B to obtain a liquid C; sterilizing, degassing, cooling and filling the liquid C to obtain the transparent protein electrolyte sports drink. The prepared drink has high electrolyte content, attractive color, refreshing taste and no astringent feeling, and presents bright transparent pink within 6 months of normal temperature shelf life.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of food technology, in particular to a transparent protein electrolyte sports drink and a preparation method thereof. BACKGROUND

[0002] In early 2023, sports drinks with electrolytes as key ingredients saw a surge in demand as they helped alleviate dehydration caused by symptoms such as fever. The focus on sports scenes and active lifestyles have contributed to the growth of the sports drink market.

[0003] Physical activity is a catalyst for the metabolic process of the human body. Once the body enters a state of exercise, the energy metabolism and material metabolism of the body will be enhanced. In order to maintain the energy supply and heat balance of the body during exercise, a large amount of energy material is consumed, and various electrolytes are lost with sweat. The consumption of energy materials and the loss of electrolytes are important reasons for the decline of human performance in sports. Studies have shown that the supplementation of various nutrients required during exercise during exercise is beneficial to delay the onset of fatigue and accelerate fatigue recovery.

[0004] Sports drinks are formulated according to the physiological consumption characteristics during exercise, can targetedly supplement part of the nutrients lost during exercise, play a role in maintaining and improving exercise capacity, and accelerating the elimination of post-exercise fatigue. Commonly added substances include carbohydrates, vitamins, proteins, electrolytes, etc. The demand for sports drinks by the human body is to quickly replenish water, energy and electrolytes, and to help alleviate and eliminate post-exercise muscle fatigue. Therefore, sports drinks contain an appropriate amount of monosaccharides or disaccharides to provide energy; need to contain an appropriate amount of electrolytes such as sodium, potassium and calcium to supplement the loss of electrolytes due to exercise sweating; in addition, an appropriate amount of protein is needed to repair muscle damage, relieve muscle fatigue, and improve exercise capacity.

[0005] Commercially available sports drink products are basically some electrolyte drinks simply added with sugars, vitamins, and minerals. However, the current sports drinks still have the following problems: (1) the speed of quenching thirst and eliminating fatigue is not ideal; (2) the supplemented electrolytes are not easily absorbed by the human body due to the interference and repulsion between certain elements; (3) the palatability of sports drinks is not good, with an unpleasant flavor and poor taste, which cannot meet the requirements of various individuals in improving exercise capacity and promoting health.

[0006] Recently, more and more evidence shows that whey protein plays an important role in improving exercise capacity and relieving post-exercise muscle fatigue, but there is still no whey protein sports drink on the market. Whey protein is rich in branched-chain amino acids (BCAA) that promote muscle growth and can be used as a nutritional ingredient for sports fitness functional foods, which plays an important role in improving exercise capacity and relieving post-exercise muscle fatigue.

[0007] When whey protein is applied in sports drinks, the heat resistance of whey protein is poor, and flocculation, stratification, precipitation, denaturation and other phenomena are prone to occur during the production process and product storage process, and the stability is poor.

[0008] Therefore, it is urgent to provide an electrolyte sports drink containing whey protein with strong stability and good taste. SUMMARY

[0009] In view of the above-mentioned shortcomings of the prior art, the purpose of the present application is to provide a transparent protein electrolyte sports drink and a preparation method thereof, which solves the problem of poor stability of whey protein in transparent electrolyte sports drinks in the prior art.

[0010] In order to achieve the above-mentioned purposes and other related purposes, the present application is obtained by the following technical solutions.

[0011] The first aspect of the present application is to provide a transparent protein electrolyte sports drink, which comprises the following raw materials in percentage by weight: nanofiltration filtrate 80-85%, sugar 4.5-5.5%, decolorized and deacidified apple concentrate juice 2.0-3.0%, whey protein 1.2-3.2%, acidity regulator 0.15-0.25%, blueberry extract 0.20-0.30%, needle cherry concentrate powder 0.20-0.30%, natural lemon essence 0.15-0.20%, and water to make up to 100%; the acidity regulator is selected from one or more of citric acid or malic acid.

[0012] The second aspect of the present application is to provide a preparation method of a transparent protein electrolyte sports drink as described above, comprising the following steps:

[0013] (1) mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain a liquid A; the mixing rate is <350 rpm / min;

[0014] (2) mixing decolorized and deacidified apple concentrate juice, acidity regulator, blueberry extract, needle cherry concentrate powder and water to obtain a liquid B;

[0015] (3) mixing the liquid A obtained in step (1) after cooling and the liquid B obtained in step (2) to obtain a liquid C;

[0016] (4) steam immersion ultra-high temperature instantaneous sterilization, degassing, cooling and filling of the liquid C obtained in step (3) to obtain a transparent protein electrolyte sports drink.

[0017] As described above, the transparent protein electrolyte sports drink and the preparation method thereof of the present application have the following beneficial effects:

[0018] (1) The application provides a preparation method of a transparent protein electrolyte sports drink, which combines milk nanofiltration filtrate, whey protein and acai berry extract, so that the electrolyte content of the finally prepared product is 2-3 times higher than that of traditional sports drinks, and the product has the functions of water replenishment, electrolyte replenishment, muscle damage repair and fatigue relief;

[0019] (2) The raw materials of the transparent protein electrolyte sports drink of the application are all natural food sources, safe and healthy, less irritating to the human body, and easy to be absorbed by the human body;

[0020] (3) The preparation method of the transparent protein electrolyte sports drink of the application, the sports drink prepared by high-temperature sterilization is bright and transparent pink within a shelf life of 6 months at room temperature, the color is attractive, the taste is refreshing without astringency, the flavor is good, and the stability is good, which solves the problems of the stability of whey protein in a complex electrolyte solution and the discoloration of acai berry under heat. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 The product schematic diagram of Example 5 of the application is shown.

[0022] Figure 2 The product schematic diagram of Comparative Example 5 of the application is shown.

[0023] Figure 3 The product schematic diagram of Comparative Example 3 stored for 30 days of the application is shown.

[0024] Figure 4 The product schematic diagram of Comparative Example 3 stored for 40 days of the application is shown. DETAILED DESCRIPTION

[0025] In order to make the invention purpose, technical scheme and beneficial technical effects of the application clearer, the application is further described in detail below in combination with examples, and those skilled in the art can easily understand other advantages and effects of the application from the contents disclosed in the description.

[0026] The first aspect of the application is to provide a transparent protein electrolyte sports drink, which comprises the following raw materials in percentage by weight: nanofiltration filtrate 80-85%, sugar 4.5-5.5%, deacidified and decolorized apple concentrate 2.0-3.0%, whey protein 1.2-3.2%, acidity regulator 0.15-0.25%, acai berry extract 0.20-0.30%, needle cherry concentrate powder 0.20-0.30%, natural lemon essence 0.15-0.20%, and water to make up to 100%; the acidity regulator is selected from one or more of citric acid and malic acid.

[0027] The whey protein of the present application is rich in branched chain amino acids (BCAA) which can promote muscle growth, and can be used as a nutritional component of a sports fitness functional food, and has an important role in improving exercise capacity and relieving muscle fatigue after exercise. The whey protein beverage is a sports functional beverage containing whey protein, generally in a transparent or translucent state, has high nutritional value, and is easy to absorb. The transparent whey protein beverage is increasingly favored by consumers due to its clear and transparent state.

[0028] The nanofiltration filtrate of the present application contains a large amount of natural electrolytes such as sodium, potassium, calcium, magnesium and phosphorus, which are nutrients required by sports drinks, and can meet the needs of rapid water and electrolyte supplementation.

[0029] During intense exercise, a large number of free radicals are produced in the body, causing oxidative stress, which can damage cells and accelerate aging. The acai berry extract of the present application is rich in powerful antioxidant substances, which can effectively neutralize these free radicals and reduce oxidative stress, thereby protecting muscles and other cells from damage. However, anthocyanins in acai berries are easily affected by heat and light, resulting in loss of the original bright pink color.

[0030] The present application can be used to prepare a transparent protein electrolyte sports drink that can supplement water, electrolytes, repair muscle damage and relieve fatigue by combining nanofiltration filtrate, whey protein and acai berry extract. The nanofiltration filtrate can supplement water and electrolytes, the whey protein can repair muscle damage, and the acai berry extract contains powerful antioxidant substances that can eliminate inflammation caused by exercise damage and protect muscles. Therefore, the combination of the three main functional ingredients can comprehensively solve the problems of water and electrolyte supplementation, repair of exercise-induced muscle damage, muscle protection and relief of exercise-induced fatigue.

[0031] In some embodiments of the present application, the sports drink comprises the following raw materials in the following weight percentages: nanofiltration filtrate 80-83%, sugar 4.5-5%, decolorized and deacidified apple concentrate 2.0-2.5%, whey protein 1.2-2.2%, acidity regulator 0.15-0.2%, acai berry extract 0.20-0.25%, needle cherry concentrate powder 0.20-0.25%, natural lemon essence 0.15-0.17%, and water to make up the rest to 100%.

[0032] In some embodiments of the present application, the sports drink comprises the following raw materials in the following weight percentages: nanofiltration filtrate 83-85%, sugar 5-5.5%, decolorized and deacidified apple concentrate 2.5-3.0%, whey protein 2.2-3.2%, acidity regulator 0.2-0.25%, acai berry extract 0.25-0.30%, needle cherry concentrate powder 0.25-0.30%, natural lemon essence 0.17-0.20%, and water to make up the rest to 100%.

[0033] In some embodiments of the present application, the sports beverage comprises the following raw materials by weight percentage: nanofiltration filtrate 80%, sugar 4.5%, decolorized and deacidified apple concentrate 2.0%, whey protein 1.2%, acidity regulator 0.15%, blueberry extract 0.20%, acerola cherry concentrate powder 0.20%, natural lemon essence 0.15%, and water to make up 100%.

[0034] In some embodiments of the present application, the sports beverage comprises the following raw materials by weight percentage: nanofiltration filtrate 82%, sugar 5.25%, decolorized and deacidified apple concentrate 2.5%, whey protein 2.2%, acidity regulator 0.2%, blueberry extract 0.25%, acerola cherry concentrate powder 0.25%, natural lemon essence 0.18%, and water to make up 100%.

[0035] In some embodiments of the present application, the sports beverage comprises the following raw materials by weight percentage: nanofiltration filtrate 85%, sugar 5.5%, decolorized and deacidified apple concentrate 3.0%, whey protein 3.2%, acidity regulator 0.25%, blueberry extract 0.30%, acerola cherry concentrate powder 0.30%, natural lemon essence 0.20%, and water to make up 100%.

[0036] In some embodiments of the present application, the nanofiltration filtrate is the filtrate of skimmed raw cow milk after nanofiltration membrane filtration. The present application does not have special requirements for the source of skimmed raw cow milk, and commercially available products of skimmed raw cow milk commonly used by those skilled in the art can be used. The pore size of the nanofiltration membrane is 200-400 daltons, specifically, the pore size can be 200-300 daltons, 300-400 daltons; typically but not limited to, for example, 200 daltons, 300 daltons, 400 daltons; the filtration temperature can be 2-6℃, specifically, the temperature can be 2-4℃, 4-6℃; typically but not limited to, for example, 2℃, 4℃, 6℃. The concentration ratio of the nanofiltration membrane is 1.5-2; the potassium content of the nanofiltration filtrate is ≥150mg / 100g, the sodium content is ≥40mg / 100g, the calcium content is ≥30mg / 100g, the magnesium content is ≥8mg / 100g, the phosphorus content is ≥40mg / 100g, and the chloride ion content is ≥100mg / 100g. The nanofiltration filtrate of the present application is essentially the natural electrolyte composition of milk after removing fat, protein and lactose. Compared with directly drinking beverages with added sodium chloride and potassium chloride electrolytes, the water supplement effect and fatigue relief effect of the human body are better, the electrolyte types of the nanofiltration filtrate are more abundant, and the ratio is more suitable for living organisms.

[0037] In some embodiments of the present application, the sugar of the present application is selected from one or more of the following: superior carbonated white granulated sugar, crystalline fructose; the source of the superior carbonated white granulated sugar is not particularly limited in the present application, and the commercially available product of the superior carbonated white granulated sugar commonly used by those skilled in the art can be used. The source of the crystalline fructose is not particularly limited in the present application, and the commercially available product of the crystalline fructose commonly used by those skilled in the art can be used.

[0038] In some embodiments of the present application, the decolorized and deacidified apple juice of the present application commonly used by those skilled in the art can be used, and the source of the decolorized and deacidified apple juice concentrate is not particularly limited in the present application, and the commercially available product of the decolorized and deacidified apple juice commonly used by those skilled in the art can be used. Specifically, the soluble solids content of the decolorized and deacidified apple juice concentrate is ≥70%.

[0039] In some embodiments of the present application, the whey protein of the present application commonly used by those skilled in the art can be used, and the source of the whey protein is not particularly limited in the present application, and the commercially available product of the whey protein commonly used by those skilled in the art can be used. Specifically, the whey protein is isolated whey protein, and preferably, the protein content of the whey protein is ≥95%, and the pH value of 1% aqueous solution is 3.5-3.8. Whey protein has astringency, and the whey protein used in the present application is treated with an acidity regulator to maintain transparent texture, but at the same time, its taste also brings astringency, and when the addition amount reaches a certain degree, other tastes (acid, sweet, etc.) are difficult to mask the astringency, so the addition amount of whey protein needs to be controlled to improve the taste of the beverage.

[0040] In some embodiments of the present application, the acidity regulator is composed of citric acid and malic acid in a mass ratio of 2-4:1. Specifically, the mass ratio of citric acid and malic acid can be 2-3:1, 3-4:1; typically but not limited to, for example, 2:1, 3:1, 4:1.

[0041] In some embodiments of the present application, the acai berry extract of the present application commonly used by those skilled in the art can be used, and the source of the acai berry extract is not particularly limited in the present application, and the commercially available product of the acai berry extract commonly used by those skilled in the art can be used. Specifically, the acai berry extract is pink instant type, the total polyphenol content is ≥0.5g / 100g, and the total anthocyanin content is 150mg / 100g-300mg / 100g. When the acai berry extract is added to the sports drink, the color of the acai berry extract is basically not affected during the preparation of the sports drink, thereby giving the sports drink a bright pink color. Among them, the acai berry extract itself is dark purple, and after being diluted into a solution, it becomes purple red, and after being treated by ultra-high temperature instant sterilization, it becomes bright pink, and the color is more attractive.

[0042] In some embodiments of the present application, the acerola cherry concentrate powder of the present application can be used as the acerola cherry concentrate powder commonly used in the art, and the present application does not have special limitations on the source of the acerola cherry concentrate powder. Commercially available acerola cherry concentrate powder commonly used by those skilled in the art can be used. Specifically, the acerola cherry concentrate powder is instant type, and the content of vitamin C is ≥ 17%.

[0043] In some embodiments of the present application, the natural lemon essence can be commercially available or self-made. Specifically, the natural lemon essence of the present application is commercially available. The natural lemon essence contains lemon oil and other natural plant ingredients that help to defoam, so that the natural lemon essence has a certain defoaming effect when used, is convenient to mix with whey protein, increases the solubility of whey protein, and improves the mixing uniformity between whey protein and other raw materials.

[0044] In some embodiments of the present application, the water is softened water.

[0045] The second aspect of the present application provides a preparation method of the transparent protein electrolyte sports beverage as described above, comprising the following steps:

[0046] (1) mixing the nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain a feed solution A; the mixing rate is < 350 rpm / min;

[0047] (2) mixing the decolorized and deacidified apple concentrate juice, acidity regulator, blueberry extract, acerola cherry concentrate powder and water to obtain a feed solution B;

[0048] (3) mixing the feed solution A obtained in step (1) after cooling and the feed solution B obtained in step (2) to obtain a feed solution C;

[0049] (4) steam immersion ultra-high temperature instantaneous sterilization, degassing, cooling and filling of the feed solution C obtained in step (3) to obtain the transparent protein electrolyte sports beverage.

[0050] In some embodiments of the present application, the method and conditions of mixing in step (1) are commonly used in the art. Specifically, the temperature of mixing in step (1) is 40-50℃, the mixing time is 20-30 min, and the stirring rate is 200-300 rpm / min.

[0051] In some embodiments of the present application, the method and conditions of mixing in step (2) are commonly used in the art. Specifically, the temperature of mixing in step (2) is 20-30℃, and the mixing time is 10-20 min.

[0052] In some embodiments of the present application, the method and condition of mixing in step (3) are the same as those commonly used in the art. Specifically, the temperature of mixing in step (3) is 20-30°C; the time of mixing is 20-30 min; and the stirring rate of mixing is 200-300 rpm / min.

[0053] In some embodiments of the present application, the method and condition of cooling in step (3) are the same as those commonly used in the art. Specifically, the temperature of cooling in step (3) is 20-30°C.

[0054] In some embodiments of the present application, the method and condition of cooling in step (4) are the same as those commonly used in the art. Specifically, the temperature of cooling in step (4) is 20-30°C.

[0055] In some embodiments of the present application, the temperature of sterilization in step (4) is 140-145°C; and the time of sterilization is 1-2 s. Specifically, the temperature of sterilization can be 140-142°C, 142-145°C; typically but not exclusively, for example, 140°C, 142°C, 145°C; and the time of sterilization can be 1-1.5 s, 1.5-2 s; typically but not exclusively, for example, 1 s, 1.5 s, 2 s.

[0056] In some embodiments of the present application, the degassing in step (4) is performed under vacuum; preferably, the vacuum degree of degassing is -0.6 to -0.7 bar.

[0057] In some embodiments of the present application, the filling in step (4) is a sterile filling commonly used in the art.

[0058] The above description is only used to explain the specific embodiments of the present application. Those skilled in the art can easily understand other advantages and effects of the present application from the above description. The present application can also be implemented or applied by using different specific embodiments, and the details in the description can be modified or changed based on different views and applications without departing from the spirit of the present application.

[0059] Before further describing the specific embodiments of the present application, it should be understood that the scope of protection of the present application is not limited to the following specific embodiments; it should also be understood that the terms used in the embodiments of the present application are used to describe the specific embodiments, but not to limit the scope of protection of the present application; in the description and claims of the present application, the singular forms "one", "an" and "the" include the plural forms unless otherwise explicitly stated in the text.

[0060] When the embodiments give numerical ranges, it is understood that every numerical range given can be used interchangeably with any other numerical range given, and that every numerical range given, unless otherwise stated, can be used to select any point within the range. Unless otherwise defined, all technical and scientific terms used in the present disclosure have the same meaning as commonly understood by one of ordinary skill in the art. Any method, apparatus, material or substance similar or equivalent to those described herein can be used in the practice of the present disclosure, unless specifically stated otherwise. Unless otherwise specified, the methods and materials used in the Examples below were selected according to manufacturers' instructions or according to commonly used methods in the art.

[0061] The experimental methods in the following examples, unless otherwise specified, are selected according to the conventional methods and conditions, or according to the commodity instruction.

[0062] In the following examples, the whey protein is purchased from Shanghai Jinpalm Food Co., Ltd., citric acid and malic acid are purchased from Shanghai Jetcong Trading Co., Ltd., deacidified and decolorized apple concentrate is purchased from Deli Food and Beverage Ingredients Co., Ltd., blueberry extract, pin cherry concentrate powder and natural lemon essence are purchased from Brazil Twinwheel Co., Ltd., and crystalline fructose is purchased from Bowling Bio-technology Co., Ltd. The skimmed raw milk is purchased from Guangming Dairy Co., Ltd.

[0063] Example

[0064] Table 1: Raw material component allocation ratio of transparent protein electrolyte sports drink in Examples 1-6

[0065] Raw materials / addition amount (wt%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Nanofiltration filtrate 80 85 80 85 82 83 Sugar 5 5.5 4.5 5 5.25 4.75 Decolorized and deacidified apple concentrate juice 2 3 2 3 2.5 2.5 Whey protein 1.2 3.2 3.2 1.2 2.2 2.2 Acidity regulator 0.15 0.25 0.25 0.15 0.2 0.2 Acai berry extract 0.2 0.3 0.2 0.3 0.25 0.25 Pin cherry concentrate powder 0.2 0.3 0.2 0.3 0.25 0.25 Natural lemon essence 0.15 0.2 0.2 0.15 0.17 0.18 Water 11.1 2.25 9.45 4.9 7.18 6.67

[0066] Table 2: Raw material technical index of transparent protein electrolyte sports drink in Examples 1-6

[0067]

[0068]

[0069] Example 1

[0070] The present embodiment provides a transparent protein electrolyte sports drink, the raw material component allocation ratio is shown in Table 1, and the raw material component performance is shown in Table 2, wherein the sugar is white granulated sugar, and the acidity regulator is citric acid. The preparation method of the nanofiltration filtrate includes the following steps: filtering the skimmed raw milk through a nanofiltration membrane to obtain a nanofiltration filtrate, wherein the potassium content of the nanofiltration filtrate is ≥150 mg / 100 g, the sodium content is ≥40 mg / 100 g, the calcium content is ≥30 mg / 100 g, the magnesium content is ≥8 mg / 100 g, the phosphorus content is ≥40 mg / 100 g, and the chloride ion content is ≥100 mg / 100 g; the filtering temperature is 5°C, the concentration ratio of the nanofiltration membrane is 1.7, and the pore size of the nanofiltration membrane is 300 daltons.

[0071] The embodiment provides a preparation method of a transparent protein electrolyte sports beverage, and comprises the following steps:

[0072] (1) mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain a feed liquid A; the mixing temperature is 40 DEG C, the mixing time is 20 min, and the mixing stirring speed is 200 rpm / min;

[0073] (2) mixing decolorized and deacidified apple concentrated juice, an acidity regulator, acai extract, needle cherry concentrated powder and water to obtain a feed liquid B; the mixing temperature is 20 DEG C, the mixing time is 10 min;

[0074] (3) mixing the feed liquid A obtained in the step (1) after cooling to 20 DEG C and the feed liquid B obtained in the step (2) to obtain a feed liquid C; the mixing temperature is 20 DEG C, the mixing time is 20 min, and the mixing stirring speed is 200 rpm / min;

[0075] (4) sterilizing, degassing, cooling and filling the feed liquid C obtained in the step (3) to obtain the transparent protein electrolyte sports beverage; the sterilization mode is steam immersion type ultra-high temperature instantaneous sterilization, the sterilization temperature is 140 DEG C, the sterilization time is 1 s; the degassing is carried out under vacuum condition, the vacuum degree is -0.6 bar; and the cooling temperature is 20 DEG C.

[0076] Example 2

[0077] The embodiment provides a transparent protein electrolyte sports beverage, and the raw material component proportion is shown in Table 1, and the raw material component performance is shown in Table 2; and the difference from the example 1 lies in that the raw material component proportion is different, the acidity regulator is malic acid, and the other aspects are completely same as the example 1.

[0078] The embodiment provides a preparation method of a transparent protein electrolyte sports beverage, and comprises the following steps:

[0079] (1) mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain a feed liquid A; the mixing temperature is 40 DEG C, the mixing time is 20 min, and the mixing stirring speed is 200 rpm / min;

[0080] (2) mixing decolorized and deacidified apple concentrated juice, an acidity regulator, acai extract, needle cherry concentrated powder and water to obtain a feed liquid B; the mixing temperature is 20 DEG C, the mixing time is 10 min;

[0081] (3) mixing the feed liquid A obtained in the step (1) after cooling to 20 DEG C and the feed liquid B obtained in the step (2) to obtain a feed liquid C; the mixing temperature is 20 DEG C, the mixing time is 20 min, and the mixing stirring speed is 200 rpm / min;

[0082] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain the transparent protein electrolyte sports beverage. The sterilization is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 145℃, and the sterilization time is 2s; the degassing is performed under vacuum, the vacuum degree is -0.7bar; and the cooling temperature is 30℃.

[0083] Example 3

[0084] This example provides a transparent protein electrolyte sports beverage, the raw material component ratio is shown in Table 1, and the raw material component performance is shown in Table 2. The difference from Example 1 is that the raw material component ratio is different, the sugar is crystalline fructose, the acidity regulator is composed of citric acid and malic acid at a mass ratio of 4:1, and the others are completely the same as Example 1.

[0085] This example provides a preparation method of a transparent protein electrolyte sports beverage, comprising the following steps:

[0086] (1) mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain liquid A; the mixing temperature is 50℃, the mixing time is 20min, and the mixing stirring rate is 200rpm / min;

[0087] (2) mixing decolorized and deacidified apple concentrate juice, acidity regulator, blueberry extract, needle cherry concentrate powder and water to obtain liquid B; the mixing temperature is 30℃, the mixing time is 10min;

[0088] (3) mixing liquid A obtained in step (1) cooled to 30℃ and liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 30℃, the mixing time is 20min, and the mixing stirring rate is 200rpm / min;

[0089] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain the transparent protein electrolyte sports beverage. The sterilization is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 145℃, and the sterilization time is 1s; the degassing is performed under vacuum, the vacuum degree is -0.7bar; and the cooling temperature is 20℃.

[0090] Example 4

[0091] This example provides a transparent protein electrolyte sports beverage, the raw material component ratio is shown in Table 1, and the raw material component performance is shown in Table 2. The difference from Example 1 is that the raw material component ratio is different, the sugar is crystalline fructose, the acidity regulator is composed of citric acid, malic acid at a mass ratio of 2:1, and the others are completely the same as Example 1.

[0092] This example provides a preparation method of a transparent protein electrolyte sports beverage, comprising the following steps:

[0093] (1)Mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain feed liquid A; the temperature of mixing is 40℃, the time of mixing is 30min, and the stirring rate of mixing is 300rpm / min;

[0094] (2)Mixing decolorized and deacidified apple concentrated juice, acidity regulator, blueberry extract, needle cherry concentrated powder and water to obtain feed liquid B; the temperature of mixing is 20℃, the time of mixing is 20min;

[0095] (3)Mixing feed liquid A obtained in step (1) after cooling to 20℃ and feed liquid B obtained in step (2) to obtain feed liquid C; the temperature of mixing is 20℃, the time of mixing is 30min, and the stirring rate of mixing is 300rpm / min;

[0096] (4)Sterilizing, degassing, cooling and filling feed liquid C obtained in step (3) to obtain the transparent protein electrolyte sports beverage. The sterilization is steam immersion type ultra-high temperature instantaneous sterilization, the temperature of sterilization is 140℃, and the time of sterilization is 2s; the degassing is carried out under vacuum condition, the vacuum degree is-0.6bar; and the temperature of cooling is 30℃.

[0097] Example 5

[0098] The embodiment provides a transparent protein electrolyte sports beverage, and the component proportion of raw materials is shown in Table 1, and the performance of raw materials is shown in Table 2. The difference from the embodiment 1 is that the component proportion of raw materials is different, the acidity regulator is composed of citric acid and malic acid at a mass ratio of 3:1, and the other conditions are the same as those in the embodiment 1.

[0099] The embodiment provides a preparation method of a transparent protein electrolyte sports beverage, and the method comprises the following steps:

[0100] (1)Mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain feed liquid A; the temperature of mixing is 45℃, the time of mixing is 25min, and the stirring rate of mixing is 250rpm / min;

[0101] (2)Mixing decolorized and deacidified apple concentrated juice, acidity regulator, blueberry extract, needle cherry concentrated powder and water to obtain feed liquid B; the temperature of mixing is 25℃, the time of mixing is 25min;

[0102] (3)Mixing feed liquid A obtained in step (1) after cooling to 25℃ and feed liquid B obtained in step (2) to obtain feed liquid C; the temperature of mixing is 25℃, the time of mixing is 25min, and the stirring rate of mixing is 250rpm / min;

[0103] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain the transparent protein electrolyte sports beverage. The sterilization is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 142°C, and the sterilization time is 1.5 s; the degassing is performed under vacuum, the vacuum degree is -0.65 bar; and the cooling temperature is 25°C. The product diagram is shown in FIG. (1).

[0104] Example 6

[0105] The example provides a transparent protein electrolyte sports beverage, the raw material component ratio is shown in Table 1, and the raw material component performance is shown in Table 2. The difference from Example 1 is that the raw material component ratio is different, the sugar is crystalline fructose, the acidity regulator is composed of citric acid and malic acid at a mass ratio of 3:1, and the others are completely the same as Example 1.

[0106] The example provides a preparation method of a transparent protein electrolyte sports beverage, comprising the following steps:

[0107] (1) mixing nanofiltration filtrate, whey protein, sugar and natural lemon essence to obtain liquid A; the mixing temperature is 45°C, the mixing time is 25 min, and the mixing stirring rate is 250 rpm / min;

[0108] (2) mixing decolorized and deacidified apple concentrate juice, acidity regulator, blueberry extract, needle cherry concentrate powder and water to obtain liquid B; the mixing temperature is 25°C, the mixing time is 25 min;

[0109] (3) mixing liquid A obtained in step (1) cooled to 25°C and liquid B obtained in step (2) to obtain liquid C; the mixing temperature is 25°C, the mixing time is 25 min, and the mixing stirring rate is 250 rpm / min;

[0110] (4) sterilizing, degassing, cooling and filling the liquid C obtained in step (3) to obtain the transparent protein electrolyte sports beverage. The sterilization is steam immersion ultra-high temperature instantaneous sterilization, the sterilization temperature is 143°C, and the sterilization time is 1.5 s; the degassing is performed under vacuum, the vacuum degree is -0.65 bar; and the cooling temperature is 25°C.

[0111] Comparative Example

[0112] Comparative Example 1

[0113] The comparative example provides a preparation method of a transparent protein electrolyte sports beverage, which is different from Example 5 in that the natural lemon essence in step (1) is added in step (2), and the specific steps are as follows:

[0114] (1) mixed the nanofiltration filtrate, whey protein and sugar to obtain a feed liquid A; the temperature of mixing was 45℃, the mixing time was 25 min, and the mixing stirring rate was 250 rpm / min;

[0115] (2) mixed the decolorized and deacidified apple concentrated juice, acidity regulator, acai extract, needle cherry concentrated powder, natural lemon essence and water to obtain a feed liquid B; the temperature of mixing was 25℃, the mixing time was 25 min;

[0116] Steps (3) and (4) were completely the same as those in Example 5. The raw materials in step (1) generated a large amount of foam and overflowed a lot during mixing, thereby affecting the mixing and dissolution of the whey protein, and batch production could not be realized.

[0117] Comparative Example 2

[0118] The present comparative example provided a preparation method of a transparent protein electrolyte sports beverage, which was different from Example 5 in that the stirring rate in step (1) was 350 rpm / min, and other aspects were completely the same as those in Example 5. The stirring rate in step (1) was too large, thereby causing the raw materials in step (1) to generate a large amount of foam during mixing, so that the raw materials could not be mixed and dissolved, and batch production could not be realized.

[0119] Comparative Example 3

[0120] The present comparative example provided a transparent protein electrolyte sports beverage, which was different from Example 5 in that the needle cherry concentrated powder was not added, and other aspects were completely the same as those in Example 5. The product prepared in Comparative Example 3 gradually faded in color during the shelf life, and was dark yellow after about 3 months, and was light yellow after about 4 months, so that the technical effect of the present application was difficult to achieve. The schematic diagram of the product is shown in Figure 3 and Figure 4 .

[0121] Comparative Example 4

[0122] The present comparative example provided a preparation method of a transparent protein electrolyte sports beverage, which was different from Example 5 in that step (4) was not subjected to degassing treatment, and specifically:

[0123] Steps (1)-(3) were completely the same as those in Example 5;

[0124] (4) sterilized, cooled and filled the feed liquid C obtained in step (3) to obtain a transparent protein electrolyte sports beverage. The sterilization was steam immersion type ultra-high temperature instantaneous sterilization, the sterilization temperature was 143℃, the sterilization time was 1.5 s; and the cooling temperature was 25℃.

[0125] The product prepared in Comparative Example 4 gradually changed to dark yellow after 3 months at room temperature, and was light yellow after about 5 months, so that the present application could not be realized.

[0126] Comparative Example 5

[0127] The present comparative example provides a preparation method of a transparent protein electrolyte sports drink, which is different from Example 5 in that the sterilization method in step (4) is ordinary tubular ultra-high temperature sterilization, and other aspects are completely the same as Example 5. The electrolyte sports drink prepared in the present comparative example has a dark red color. The schematic diagram of the prepared product is shown in Figure 2 .

[0128] Comparative Example 6

[0129] The present comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that the acidity regulator is lactic acid, and other aspects are completely the same as Example 5.

[0130] Comparative Example 7

[0131] The present comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that the natural lemon essence is replaced with an equal amount of natural grapefruit essence, and other aspects are completely the same as Example 5.

[0132] Comparative Example 8

[0133] The present comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that the addition amount of whey protein is 4.2%, and other aspects are completely the same as Example 5.

[0134] Comparative Example 9

[0135] The present comparative example provides a transparent protein electrolyte sports drink, which is different from Example 5 in that no decolorized and deacidified apple concentrate is added, and other aspects are completely the same as Example 5.

[0136] Performance test

[0137] I. Protein, electrolyte, and chlorine content determination

[0138] (1) Protein determination: The transparent protein electrolyte sports drinks prepared in Examples 1-6 were taken, and the protein content in the final product was detected according to the detection method in GB5009.5-2010 “National Food Safety Standard-Determination of Protein in Foods”, and the detection results are shown in Table 3.

[0139] (2) Electrolyte determination: The transparent protein electrolyte sports drinks prepared in Examples 1-6 were taken, and the electrolyte element content in the final product was detected according to the detection method in GB5009.268-2016 “National Food Safety Standard-Determination of Multi-elements in Foods”, and the detection results are shown in Table 3.

[0140] (3) Chlorine content determination: The transparent protein electrolyte sports drink prepared in Examples 1-6 was taken, and the chlorine content in the final product was determined according to the detection method in GB5009.44-2016 "National food safety standard Determination of chloride in food", and the detection results are shown in Table 3.

[0141] Table 3 Protein, potassium, sodium, calcium, magnesium, phosphorus, and chlorine content detection in the final product of Examples 1-6

[0142] Item per 100 ml Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Protein 1.14g 3.04g 3.04g 1.14g 2.09g 2.09g Potassium 120 mg 135 mg 122 mg 130 mg 126 mg 125 mg Sodium 33 mg 41 mg 34 mg 37 mg 35 mg 36 mg Calcium 20 mg 28 mg 23 mg 27 mg 25 mg 26 mg Magnesium 6.0 mg 7.0 mg 6.2 mg 6.8 mg 6.5 mg 6.6 mg Phosphorus 35 mg 45 mg 39 mg 42 mg 40 mg 41 mg Chlorine 90 mg 97 mg 92 mg 95 mg 93 mg 90 mg

[0143] It can be seen from the data in Table 3 that the protein content and electrolyte content of the sports drink prepared in Examples 1-6 are 2-3 times that of traditional sports drinks per 100 ml.

[0144] II. Sensory evaluation

[0145] The transparent protein electrolyte sports drinks prepared in Examples 1-6 and Comparative Examples 5-9 were subjected to sensory evaluation. Specifically, 30 female professional sensory evaluation personnel aged 20-30 years were selected to evaluate 4 indicators including color and transparency, sourness and sweetness, taste, and aroma, and the total score of the 4 indicators was taken as the sample evaluation score, and the average score of 30 people was taken as the final evaluation score. The evaluation rules are shown in Table 4, and the experimental results are shown in Table 5.

[0146] Table 4 Sensory evaluation standard

[0147]

[0148] Table 5 Performance test results of transparent protein electrolyte sports drinks of Examples 1-6 and Comparative Examples 5-9

[0149]

[0150]

[0151] It can be seen from the data in Table 5 that the products prepared in Examples 1-6 of the present application are highly praised in terms of color and transparency, sourness and sweetness, astringency, refreshing, and aroma. The product is clear and transparent, and has a bright pink color that is attractive; the taste is refreshing without astringency, and the sourness and sweetness are moderate; the lemon flavor is fresh and there is no odor. Comparative Example 5 is dark red and cannot achieve the bright pink color required by the present application. The scores of Comparative Example 6 in terms of sourness and sweetness, Comparative Example 7 in terms of flavor, and Comparative Example 9 in terms of taste are all lower than those of the product prepared in Example 5, and there is also a large gap in the overall score between Example 5 and Comparative Examples 5-7 and Comparative Example 9. Comparative Example 8 added more whey protein, and the scores of the product prepared therefrom in each item and the overall score are all poor.

[0152] III. Stability evaluation

[0153] The transparent egg white electrolyte sports drink prepared in Examples 1-6 and Comparative Examples 3-4 was subjected to accelerated shelf life stability observation at 37°C. The color and transparency of the product were observed at rest. The 37°C accelerated experiment was performed for 10 days, 20 days, 30 days, 40 days, 50 days, and 60 days, which correspond to 1 month, 2 months, 3 months, 4 months, 5 months, and 6 months of stability at room temperature (20-25°C). It was determined that the product was stable for 6 months at room temperature. The results are shown in Table 6.

[0154] Table 6 Transparent egg white electrolyte sports drink prepared in Examples 1-6 and Comparative Examples 3-4

[0155]

[0156]

[0157] As can be seen from the data in Table 6, the products prepared in Examples 1-6 were clear and transparent and basically maintained a bright pink color after 60 days of 37°C accelerated shelf life stability observation. The products prepared in Comparative Examples 3-4 were dark yellow and light yellow after 60 days of 37°C accelerated shelf life stability observation.

[0158] The above examples are merely illustrative of the principles of the present application and its efficacy, and are not intended to limit the present application. Any person skilled in the art who is not departing from the spirit and scope of the present application can modify or change the above examples. Therefore, all equivalent modifications or changes made by those skilled in the art without departing from the spirit and technical thought disclosed by the present application should be covered by the claims of the present application.

Claims

1. A method for preparing a transparent protein electrolyte sports drink, characterized in that, The sports drink is made from the following ingredients by weight percentage: 80-85% nanofiltration filtrate, 4.5-5.5% sugar, 2.0-3.0% decolorized and deacidified apple concentrate, 1.2-3.2% whey protein, 0.15-0.25% acidity regulator, 0.20-0.30% acai berry extract, 0.20-0.30% acerola cherry concentrate, 0.15-0.20% natural lemon flavoring, and water to 100%; the acidity regulator is selected from one or both of citric acid and malic acid; the nanofiltration filtrate is the filtrate obtained by filtering skimmed raw milk through a nanofiltration membrane, and the preparation method includes the following steps: (1) Mix nanofiltration filtrate, whey protein, sugar and natural lemon flavoring to obtain liquid A; the mixing and stirring speed is 200-300 rpm; (2) Mix decolorized and deacidified apple concentrate, acidity regulator, acai berry extract, acerola cherry concentrate and water to obtain liquid B; (3) Cool the liquid A obtained in step (1) and mix it with the liquid B obtained in step (2) to obtain liquid C; (4) The liquid C obtained in step (3) is steam-immersed in ultra-high temperature instantaneous sterilization, degassing, cooling and filling to obtain a transparent protein electrolyte sports drink.

2. The method for preparing a transparent protein electrolyte sports drink according to claim 1, characterized in that: The mixing temperature in step (1) is 40-50℃ and the mixing time is 20-30min.

3. The method for preparing a transparent protein electrolyte sports drink according to claim 1, characterized in that: The sterilization temperature in step (4) is 140-145℃; the sterilization time is 1-2s.

4. The method for preparing a transparent protein electrolyte sports drink according to claim 1, characterized in that: It also includes one or more of the following technical features: 1) The mixing temperature in step (2) is 20-30℃, and the mixing time is 10-20 min; 2) The mixing temperature in step (3) is 20-30℃; the mixing time is 20-30 min; 3) The cooling temperature in step (3) is 20-30℃; 4) The cooling temperature in step (4) is 20-30℃.

5. The method for preparing a transparent protein electrolyte sports drink according to claim 1, characterized in that: In step (4), degassing is carried out under vacuum conditions.

6. The method for preparing a transparent protein electrolyte sports drink according to claim 1, characterized in that: It also includes one or more of the following features: A1) The nanofiltration filtrate contains ≥150mg / 100g potassium, ≥40mg / 100g sodium, ≥30mg / 100g calcium, ≥8mg / 100g magnesium, ≥40mg / 100g phosphorus, and ≥100mg / 100g chloride ion. A2) The nanofiltration membrane filtration temperature is 2-6℃; A3) The nanofiltration membrane has a pore size of 200-400 Daltons.

7. The method for preparing a transparent protein electrolyte sports drink according to claim 1, characterized in that: It also includes one or more of the following features: B1) The soluble solids content of the decolorized and deacidified apple concentrate is ≥70%; B2) The whey protein mentioned is isolated whey protein; B3) The acidity regulators mentioned are citric acid and malic acid; B4) The total polyphenol content of the acai berry extract is ≥0.5g / 100g, and the total anthocyanin content of the acai berry extract is 150mg / 100g~300mg / 100g; B5) The acerola cherry concentrate contains ≥17% vitamin C; The water described in B6 is softened water.

8. The method for preparing a transparent protein electrolyte sports drink according to claim 1, characterized in that: The acidity regulator is composed of citric acid and malic acid in a mass ratio of 2-4:

1.

9. The method for preparing a transparent protein electrolyte sports drink according to claim 7, characterized in that: In feature B3), the whey protein has a protein content of ≥95%, and the pH value of a 1% aqueous solution of the whey protein is 3.5~3.

8.

10. A transparent protein electrolyte sports drink, characterized in that: It is prepared using the method described in any one of claims 1-9 for the preparation of a transparent protein electrolyte sports drink.

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