Technical formula and preparation method of medicinal and edible multifunctional anthocyanin health-preserving beverage

Abstract

The invention relates to a general situation of a product, namely a technical formula and a preparation method of a medicinal and edible multifunctional anthocyanin health-preserving beverage. 2, the invention solves the substantive problems of users, and provides a medicinal and edible formula capable of solving depression and visual impairment; the tea has the effects of regulating three highs, reducing blood pressure, reducing blood fat, reducing blood sugar, treating cardiovascular and cerebrovascular diseases, treating apoplexy, nourishing and protecting liver, treating kidney deficiency, treating mental and physical exhaustion, enhancing intelligence, improving immunity, treating lung cough, treating stomachache, treating mental and physical exhaustion, maintaining beauty and keeping young, resisting aging, having a cell regeneration function, supplementing protein, resisting cancer, diabetes, cancer and the like. And 3, the chronic diseases puzzle the normal life of people for a long time, and the physical and psychological health is in vicious circle. And 4, the problem is solved from the root of the disease, so that the user is not wound and painful by the disease. And 5, the user is helped to change from a chronic disease and sub-healthy state to a healthy state, so that the body and mind keep a healthy and virtuous circle.

Description

Technical Field

[0001] The technical formula and preparation method of a multifunctional anthocyanin health drink that is both food and medicine, specifically involving a formula that is both food and medicine that can solve depression and visual impairment. Background Technology

[0002] According to a global survey released by the World Health Organization, there are as many as 350 million people with depression worldwide. Among them, the lifetime prevalence of depressive disorders among adults in my country is 6.8%, of which depression accounts for 3.4%.

[0003] There are over 2.2 billion people worldwide with visual impairment or blindness, of whom at least 1 billion could have had their vision impairment prevented, but the problem has not yet been properly addressed.

[0004] The number of people with visual impairments is a major public health issue globally. According to the World Health Organization's (WHO) 2019 World Report on Vision, approximately 2.2 billion people worldwide have visual impairments, of which at least 1 billion are preventable and treatable. In addition, there are nearly 45 million blind people globally.

[0005] The main health problems faced by people with visual impairments include cataracts, trachoma, and glaucoma.

[0006] Most existing health drinks cannot meet users' health needs, and long-term consumption can lead to health imbalances and potential health risks.

[0007] Inventing a multifunctional anthocyanin health drink that is both medicinal and edible is a current research focus. Summary of the Invention

[0008] This invention addresses a substantial problem for users: a formula derived from both food and medicine that can resolve depression and visual impairment. Furthermore, it regulates the three highs (high blood pressure, high cholesterol, and high blood sugar), lowers blood lipids, lowers blood sugar, treats cardiovascular and cerebrovascular diseases, stroke, nourishes and protects the liver, alleviates kidney deficiency, relieves physical and mental fatigue, enhances intelligence, boosts immunity, treats coughs, stomach aches, fatigue, beautifies the skin, has anti-aging properties, promotes cell regeneration, supplements protein, and has anti-cancer effects, particularly for diabetes and cancer.

[0009] These chronic diseases have long plagued people's normal lives, putting their physical and mental health in a vicious cycle.

[0010] This invention aims to solve the problem at its root, freeing users from the torment and suffering caused by disease.

[0011] This invention helps users transition from chronic diseases and sub-health to a healthy state, allowing their mind and body to maintain a healthy and virtuous cycle.

[0012] This invention addresses the shortcomings of existing technologies by providing a solution that comprehensively addresses users' chronic diseases and sub-health conditions.

[0013] This invention utilizes an anthocyanin-rich, organically sourced, selenium-enriched, and medicinally derived anthocyanin-based health drink, which boasts powerful effects and enhanced nutritional value.

[0014] Table of efficacy and nutritional components;

[0015] Group 1: S1.1, 5-50 portions of anthocyanin juice extracted from plants and foods rich in organic selenium-enriched anthocyanins.

[0016] S1.2 Anthocyanins are water-soluble flavonoid compounds with a core structure of benzopyran salt (C6-C3-C6 skeleton).

[0017] S1.3 mainly contains the following elements: carbon (C), hydrogen (H), oxygen (O), potassium (K), magnesium (Mg), calcium (Ca), selenium (Se), etc.

[0018] S1.4 Calculation results of organic selenium-enriched anthocyanin content: Serial Number Sample number Total anthocyanin content (mg / g dry weight) Selenium content (mg / kg dry weight) Organic selenium percentage (%) 1 S001 17.2±0.9 0.85±0.05 82.3％ 2 S002 10.7±0.6 0.10±0.02 15.0％

[0019] Group 2: S2.1, organic selenium-enriched Cornus officinalis 7-19 parts, Ziziphus jujuba var. spinosa 5-26 parts, Polygala tenuifolia 0.5-19 parts, Saffron 0.1-5 parts, Cassia tora 0.3-20 parts, Oyster 5-32 parts, Ganoderma lucidum 3-36 parts, Pearl 3-20 parts, Dendrobium officinale 6-28 parts, Lemon 8-30 parts.

[0020] S2.2 The second group of the present invention contains essential elements that are indispensable to the human body: calcium (Ca), magnesium (Mg), phosphorus (P), sodium (Na), zinc (Zn), selenium (Se), iron (Fe), copper (Cu), iodine (I), potassium (K), and chromium (Cr).

[0021] S2.3, enriched with zinc (71.10 mg / kg), selenium (1.07 mg / kg), iron, etc., participates in antioxidant and immune regulation.

[0022] S2.4 contains up to 1200mg of Vitamin C, which can promote collagen synthesis and maintain the healthy growth of bones and teeth.

[0023] S2.5 DNA / RNA promotes liver metabolism, protein synthesis, and repairs liver damage.

[0024] S2.6, Ganoderic acids (Ganoderic acids A, B, C, etc.): have anti-inflammatory, hepatoprotective, and antitumor activities, and contain characteristic groups such as β-CH3 and α-CH3.

[0025] S2.7, The second group of the present invention contains 33 kinds of amino acids / nucleotides, including (aspartic acid, glutamic acid, γ-aminobutyric acid) lysine, leucine, amino acids required by the body, accounting for 30%-40% of the total amount, as well as polypeptide components.

[0026] S2.8, Formula for calculating the mass of Vitamin C;

[0027] S2.9 Calculation of lysine content (HPLC method);

[0028] S2.10, Sample peak area: 8500, Lysine standard curve equation: y = 980x + 120, Dilution factor: 10, Sample mass: 2g

[0029] S2.11

[0030] S2.12, Second group of the present invention: Amino acid / nucleotide data sequence;

[0031] Group 3: S3.1, 10-20 parts organic selenium-enriched kudzu root, 1-18 parts polygonatum, 3-15 parts angelica, 5-27 parts organic selenium-enriched codonopsis, 8-26 parts astragalus, 3-29 parts he shou wu, 2-18 parts peach kernel, 5-18 parts poria, 6-29 parts organic selenium-enriched hawthorn, 2-21 parts American ginseng, 5-18 parts codonopsis, 3-20 parts red ginseng, 3-18 parts fritillaria, 1-16 parts asparagus, 3-12 parts anemarrhena, 5-22 parts motherwort, 6-20 parts chicken liver.

[0032] S3.2 The third group of the present invention is rich in trace elements: manganese (Mn), selenium (Se), zinc (Zn), iron (Fe), calcium (Ca), phosphorus (P), potassium (K), magnesium (Mg), copper (Cu), cobalt (Co), molybdenum (Mo), nitrogen (N), and fluorine (F).

[0033] S3.3, The third group of the present invention: Daidzin and Daidzein have the effects of dilating coronary arteries, improving cerebral circulation, lowering blood sugar, and are used clinically for the treatment of cardiovascular and cerebrovascular diseases. They also have antispasmodic and antioxidant effects. Daidzein can induce tumor cell differentiation and inhibit proliferation.

[0034] S3.4, Sterols and Fatty Acids: β-Sitosterol: lowers cholesterol, prevents arteriosclerosis; arachidic acid and tetracosanoic acid are components of cell membrane structure and participate in energy metabolism; dimethoxycoumarin has anticoagulant and antibacterial properties.

[0035] S3.5, The third group of the present invention contains 30 amino acid / nucleotide components, including essential amino acids (threonine, leucine, adenosine, guanosine, adenosine diphosphate (ADP), adenosine triphosphate (ATP), etc.), with the total amino acid content accounting for 3.66% of the dry weight.

[0036] S3.6, Flavonoids: Luteolin-7-O-β-D-glucoside and luteolin-7-O-rutin glycoside, which have antioxidant and anti-inflammatory activities.

[0037] S3.7 The third group of the present invention is composed of multiple monosaccharides, such as glucose, fructose and arabinose, which have multiple effects such as regulating immunity, anti-oxidation, anti-tumor and hypoglycemia.

[0038] It can improve the body's immune function, enhance the phagocytic ability of macrophages, and promote the proliferation and differentiation of lymphocytes.

[0039] S3.8, Atractylodes lactones I, II, and III have anti-inflammatory, analgesic, gastrointestinal motility-regulating, and inflammatory mediator-inhibiting effects, reducing inflammatory responses. They also have a certain regulatory effect on gastrointestinal peristalsis and digestive function.

[0040] S3.9, caryophyllene: It has activities such as relieving asthma, expectorating, and suppressing cough, and has a certain therapeutic effect on respiratory diseases.

[0041] S3.10, Saponins: These saponins have the effects of regulating the nervous system, improving learning and memory, anti-fatigue and anti-stress, and affecting the release and metabolism of neurotransmitters, thereby having a regulatory effect on the central nervous system.

[0042] S3.11, The third group of the present invention: contains lignan compounds such as (7R,8S,7'E)-3',4-dihydroxy-3-methoxy-8,4'-oxonephrol-7'-ene-7,9,9'-triol (compound 1), which have potential anti-inflammatory and antitumor activities.

[0043] S3.12, isoflavone glycosides and santalin glycosides, contain new compounds such as 9,10-dimethoxysantalin-3-O-β-D-glucoside.

[0044] S3.13, Dextran: including water-soluble α-(1→4)(1→6) dextran and insoluble α-(1→4) dextran.

[0045] S3.14, Emodin, Physcion, Chrysophanol, Rhein and their glycoside derivatives (such as emodin-8-O-β-D-glucoside).

[0046] S3.15. Peach kernels are rich in fatty oils (about 30-50%), including unsaturated fatty acids such as oleic acid and linoleic acid, as well as sterol derivatives such as 24-methylenecycloarpineol and limonesteride.

[0047] S3.16. This invention: It has a β-(1→3) pyranose main chain and β-(1→6) glucose side chain, accounting for more than 75% of the dry weight, and inhibits mouse sarcoma S180 (tumor inhibition rate of 96.88%).

[0048] S3.17, diols are aglycones: Rb1, Rb2, Rb3, Rc, Rd and pseudoginsenoside F11, which enhance central nervous system inhibition and anti-fatigue. Pseudoginsenoside F11 has neuroprotective and antidepressant effects.

[0049] S3.18, Timosaponin N: First isolated in 2020, molecular weight C 45 H 76 O 20 It has antiviral potential (inhibiting HBV / HIV replication).

[0050] S3.19, Mangiferin: Content ≥0.7% (Pharmacopoeia requirement), its free radical scavenging ability is 100 times that of vitamin E, and it significantly reduces MDA in the liver injury model by 54%.

[0051] S3.20, This group invented polysaccharides A, B, C, and D: which regulate the phagocytic function of macrophages (ED). 50 =12.5μg / mL), anti-fatigue, liver protection RAP1, F1, F2, enhance antibody secretion, inhibit tumor metastasis (such as cervical cancer, lung cancer metastasis).

[0052] S3.21. The saponins are mainly Sarsasapogenin, and also contain Markogenin and Neogitogenin.

[0053] S3.22 contains up to 50,900 International Units (IU) of Vitamin A, far exceeding other common foods, and is crucial for vision protection and skin health.

[0054] S3.23, Group 3 of the present invention: Vitamin A (50900 IU / 100g), with a folic acid content as high as 1172.2 micrograms, helps protect vision, solves eye disease problems, improves symptoms of decreased vision and blurred vision, and reduces the occurrence of cataracts.

[0055] S3.24. People who are deficient in vitamin A for a long time may experience problems such as dry and bloodshot eyes, which can lead to corneal damage in severe cases.

[0056] S3.25, Calculation formula for essential amino acids;

[0057] S3.26, Third group of the present invention: Amino acid / nucleotide data sequence listing;

[0058] S3.27, International Units (IU) Retinol Equivalent (RAE);

[0059] S3.28, The third formula for calculating vitamin A in this invention; 50,900IU÷3.33≈15,300μg RAE

[0060] S3.29, Results of Public Announcement

[0061] The fourth group of this invention: S4.1, 0.3-10 parts of deer antler, 6-16 parts of tortoise shell, 6-28 parts of Cistanche deserticola, 3-8 parts of Gastrodia elata, 5-12 parts of organic selenium-enriched red peach K, 2-9 parts of ginseng, 10-20 parts of organic selenium-enriched yam, 8-16 parts of grass carp, 7-13 parts of cuttlefish, 6-17 parts of cuttlefish bone, 7-19 parts of organic selenium-enriched soft-shelled turtle, 17-30 parts of abalone, and 500-1000 parts of mineral water.

[0062] S4.2 Deer antlers contain 30% to 40% protein, including collagen and various essential amino acids (glycine and proline). Collagen can support bone and joint health, while amino acids participate in human metabolism and repair.

[0063] S4.3 Deer antlers contain 30% to 40% protein, including collagen and various essential amino acids (glycine and proline). Collagen supports bone and joint health, while amino acids participate in human metabolism and repair.

[0064] S4.4 Calcium phosphate: accounting for 50% to 60%, it is the main component of bone calcification, accounting for about 10% to 15%, and has the effect of growth factor (IGF-1) on cell proliferation and tissue regeneration.

[0065] S4.5, Heteropolysaccharides (GE-I, GE-II, GE-III): Composed of glucose, mannose, xylose and arabinose in a ratio of 70:1:0.5:0.3, which significantly enhances cellular immune function.

[0066] S4.6, chitinase (31.5 kDa) and β-1,3-glucanase (94 kDa) decompose the cell wall of Armillaria mellea.

[0067] S4.7, The fourth group of the present invention contains bis(4-hydroxybenzyl) ether-mono-β-D-glucopyranoside, which has unique antifungal activity and synergistic sedative effect, and can defend against fungal infection.

[0068] S4.8, Fourth group of the present invention; Anti-cancer function: Reduces cancer risk through mechanisms that induce cancer cell apoptosis and inhibit angiogenesis [citation: comprehensive inference].

[0069] S4.9. This invention has the following effects: activating macrophages and T / B lymphocytes, enhancing the body's disease resistance (enhancing NK cell activity), anti-tumor and antioxidant effects.

[0070] S4.10, selenomethionine (Se-Met), selenocysteine ​​(Se-Cys), and organic selenium-enriched yam can contain up to 0.27 mg / kg of selenium (compared to only 0.01 mg / kg for ordinary yam).

[0071] S4.11. The present invention has anti-inflammatory / balance regulation: it inhibits the NF-κB pathway, reduces the release of inflammatory factors (TNF-α, IL-6), and converts them into phytoestrogens (diosgenin), thereby relieving menopausal hot flashes and osteoporosis.

[0072] S4.12, This invention combination: The results show that 50 mg / kg of organic selenium-enriched yam polysaccharide component has a significant inhibitory effect on Lewis lung cancer.

[0073] S4.13. Enhance immunity: Promote lymphocyte proliferation and antibody synthesis, and enhance the body's resistance to viruses (such as influenza and COVID-19) and bacteria.

[0074] S4.14. Diosgenin can inhibit cell cycle arrest induced by PI3KAkt in human liver cancer cells SMMC-7721 by upregulating the expression of tumor suppressor genes p21 and p27, and induce autophagy in SMMC-7721 cells to achieve anti-cancer effects.

[0075] S4.15, diol type (Rb1, Rb2, Rc, Rd), account for more than 60% of the total saponins.

[0076] S4.16, Triol type (Rg1, Re, Rf): accounting for 20% to 30%, mainly enhancing central nervous system excitability and anti-aging activity.

[0077] S4.17 contains trace elements: calcium (Ca), phosphorus (P), zinc (Zn), copper (Cu), manganese (Mn), selenium (Se), iron (Fe), potassium (K), phosphorus (P), magnesium (Mg), germanium (Mo), and cobalt (Co). Among them, selenium and germanium have anti-cancer and immune-enhancing functions.

[0078] S4.18, The fourth group of the present invention: The protein content per 100 grams is 19.5 to 20.1 grams, containing 18 kinds of amino acids, among which essential amino acids such as leucine (1535 mg), lysine (1822 mg), and threonine (828 mg) have a high proportion, supporting tissue repair and metabolic regulation.

[0079] S4.19, Minerals: Calcium (44 mg / 100g), Phosphorus (19 mg / 100g), Iron (0.9 mg / 100g), Selenium (38.18 mcg / 100g, accounting for 63% of daily intake), Potassium (290 mg / 100g), etc., which respectively support bone health, hematopoietic function and electrolyte balance.

[0080] S4.20 (Cuttlebone): The main component is calcium carbonate (80%-85%), which has the effects of astringing and stopping bleeding, neutralizing acid and relieving pain, and can be used to treat gastric ulcer bleeding and improve anemia.

[0081] S4.21, Minerals: Calcium (44 mg / 100g), Phosphorus (196–260 mg / 100g), Iron (0.9–8.2 mg / 100g).

[0082] S4.22. The invention combination of this group has a unique high selenium content (reaching more than 38.18 micrograms / 100 grams, far exceeding the 4.46 micrograms / 100 grams of ordinary food).

[0083] S4.23, High in collagen (accounting for 30% to 50% of dry weight), sodium content reaches 2011.7 mg / 100g (far exceeding the daily recommended intake), and calcium content is 266 mg / 100g (about twice that of milk).

[0084] S4.24, Taurine and DHA / EPA: Rich in taurine and omega-3 fatty acids (DHA, EPA), which can promote nerve development and cardiovascular health.

[0085] S4.25, Calculation of protein molar concentration;

[0086] S4.26. Prepare 100 mL of 0.1 mol / L BSA (molecular weight 66,430 Da) solution. m=0.1×66.43×0.1=0.6643gm.

[0087] S4.27, Calculation method for the molar mass of calcium phosphate; Ca=40.08g / mol, P=30.97g / molP=30.97g / mol, O=16.00g / molO=16.00g / mol.

[0088] S4.28, Distribution Calculation Method; 3×Ca=3×40.08=120.24g / mol. 2×PO4=2×(30.97+4×16.00)=2×(30.97+64.00)=2×94.97=189.94g / mol. Total molar mass = 120.24 + 189.94 = 310.18 g / mol.

[0089] S4.29, Fourth group of the present invention: amino acid / nucleotide sequence;

[0090] The raw material extraction technology and the ultrasonic membrane separation technology are combined to form the Ultrasound-Assisted Membrane Separation (UAMS) technology.

[0091] Ultrasonic waves generate high-frequency vibrations in liquids, forming micron-sized bubbles (cavitation bubbles) that collapse instantly, releasing enormous energy (local temperatures can reach 5000K and pressures exceed 100MPa), which destroys large molecular aggregates or colloidal structures in the liquid and reduces deposition on the membrane surface.

[0092] Enhanced shear force: The microjets generated by cavitation can strip contaminants from the membrane surface, reduce the thickness of the concentration polarization layer, and increase transmembrane flux.

[0093] Acoustic flow effect: Ultrasonic waves induce liquid turbulence, promote solute diffusion on the membrane surface, reduce boundary layer resistance, and improve mass transfer efficiency.

[0094] Pretreatment: The feed solution is subjected to ultrasonic pretreatment (frequency 20-40kHz, power 50-200W, treatment time 5-15 minutes) to change the solute particle size or charge distribution and reduce the risk of membrane fouling.

[0095] Extraction: In the food extract, ultrasonic pretreatment reduced the molecular weight of polysaccharides from 200kDa to 50kDa, significantly reducing the fouling rate of the ultrafiltration membrane (MWCO 100kDa).

[0096] In whey protein separation, integrated ultrasonic quantum computing can increase membrane flux by 40%.

[0097] Improved anti-fouling performance: Reduced frequency of chemical cleaning (chemical cleaning agent usage in wastewater treatment reduced to 30%).

[0098] Separation efficiency optimization: AI computing power algorithm throughput is increased by 20%-50%, and energy consumption is reduced by 10%-25%.

[0099] Filtration: A three-stage filtration method is employed, including microfiltration (MF), ultrafiltration (UF), nanofiltration (NF), and reverse osmosis (RO).

[0100] Clarification: Ultrasonic-assisted microfiltration (0.1μm pore size) of beverage juice reduces turbidity to 1.5 NTU while retaining more than 90% of polyphenols.

[0101] Ultrasonic pretreatment combined with ceramic membrane filtration (MWCO 10kDa) increased the recovery rate of flavonoids from 65% to 88%.

[0102] Enzyme purification: Ultrasonic online assisted tangential flow filtration, enzyme activity retention rate of over 95%, and processing time reduced by 30%.

[0103] Oily wastewater: Ultrasonic combined with polyvinylidene fluoride (PVDF) ultrafiltration membrane, oil rejection rate >99%, flux stable at 80 L / (m³) 2 ·h).

[0104] High-salt wastewater: Low-frequency ultrasound (28kHz) delays scaling on the reverse osmosis membrane, maintaining a desalination rate of over 98.5%.

[0105] Data from mouse experiments on depression;

[0106] Mice were subjected to obstacles and anxiety, and prolonged stress (fasting, reversed day and night, damp bedding, restraint, etc.) for 5 weeks.

[0107] Week 1: Simulate depressive-like behaviors caused by long-term human stress.

[0108] Decreased preference for sugary drinks (anhedonia), weight loss, and reduced exploratory behavior.

[0109] Experimental mice were repeatedly subjected to highly aggressive attacks from their own kind to induce social avoidance and depressive-like behaviors.

[0110] Week 2: Focusing on depression induced by social stress, and the comorbidity mechanism of social anxiety and depression.

[0111] Mice were trained to feel "helpless" through inescapable electric shocks.

[0112] The reduced avoidance behavior in the experiment reflects a state of despair.

[0113] Week 3: Forced swimming test (FST) and tail suspension test (TST) were conducted to assess “behavioral despair” by observing the time the mice remained still (duration of stillness).

[0114] Forced swimming / tail suspension test: Prolonged immobility time indicates "desperate behavior".

[0115] Week 4: Insomnia / Fear: Intense anxiety, trembling, inability to fall asleep.

[0116] Social interaction test: Observe the willingness to interact with unfamiliar mice (social avoidance).

[0117] Elevated cross maze & open field experiment: to assess anxiety-like behaviors (often comorbid with depression).

[0118] Week 5: Neuroendocrine: Elevated plasma corticosterone (CORT) levels (HPA axis overactivation).

[0119] Brain tissue analysis revealed a reduction in hippocampal volume, decreased neurogenesis, and altered synaptic plasticity in the prefrontal cortex.

[0120] Inflammatory factors: Elevated levels of pro-inflammatory factors such as IL-6 and TNF-α.

[0121] Monoamine neurotransmitters: Decreased levels of 5-HT, NE, and DA in the brain (detected by HPLC and mass spectrometry).

[0122] Starting in week 6, mice were fed anthocyanin-rich health drinks, which were continued for 5 weeks.

[0123] Week 1: 100mL each time, three times a day. Mice gradually began to recover their physical strength, and their mental tremors and insomnia improved. 5-HT levels increased, and mood and sleep structure improved.

[0124] Week 2: 100 mL each time, three times a day. Despair behavior improved, symptoms of intense anxiety improved, and social ability with unfamiliar mice began to increase.

[0125] Week 3: 100 mL each time, three times a day, to inhibit HPA axis activation, reduce corticosterone levels, increase the proliferation of neural stem cells in the hippocampal dentate gyrus, and significantly increase the number of new neurons.

[0126] Week 4: 100 mL each time, three times a day, to improve treatment resistance to depression (clinical trials showed a 30% increase in effectiveness).

[0127] Week 5: 100mL each time, three times a day. Folic acid and vitamin B12: as coenzymes, they promote tryptophan hydroxylase (TPH) activity and enhance 5-HT synthesis efficiency.

[0128] The data above show that the anthocyanin health drink of the present invention, which is both food and medicine and has multiple functions, can solve the substantial problem of depression.

[0129] Data from mouse experiments on visual impairments;

[0130] The lights were bright, and the mice were trained for 4 hours in the morning and 4 hours in the afternoon by looking at the electronic screen with the lights on.

[0131] For six consecutive weeks, the mice were subjected to a reversed day-night cycle with the lights off, and the screen content was repeatedly switched between static and dynamic modes.

[0132] Through repeated experiments on mice, it was found that the disease risk varied among mice of different ages.

[0133] Physiological characteristics of young mice: ① High lens transparency, strong ultraviolet penetration, retinal damage is easy, macular area is not fully developed, and the risk of light damage is high.

[0134] Acute phototoxic reaction: ②Retinal edema, blurred vision, photophobia and tearing, which in severe cases can lead to solar retinopathy (foveal burn).

[0135] Impact: ③ Accelerates early-stage macular degeneration and speeds up the progression of myopia (accommodative spasm under strong light).

[0136] Middle-aged mice: ① The lens begins to harden, the ability to filter blue light decreases, and some mice have developed dry eye and early cataracts.

[0137] Severe eye strain: ② Increased intraocular pressure, headache, transient accommodative paralysis, and punctate corneal epithelial detachment (exacerbation of dry eye syndrome).

[0138] Blue light cumulative damage: ③ Accelerates cataracts and age-related macular degeneration (AMD), and induces or aggravates glaucoma (prolonged pupil constriction leads to impaired aqueous humor circulation).

[0139] Older mice: ① have lens opacity (cataracts), macular degeneration, and constricted pupils, requiring stronger light exposure but with poor tolerance to strong light.

[0140] Acute vision loss: ② Light damage combined with worsening cataract opacity increases the risk of retinal ischemia (arteriosclerosis + phototoxicity).

[0141] Accelerating macular atrophy: ③ Leading to irreversible central vision loss and inducing acute attacks of angle-closure glaucoma (pupil constriction under strong light, iris forward displacement blocking the angle of the anterior chamber).

[0142] Mouse model: When mice were exposed to strong light (10,000 lux) for 48 hours, the apoptosis rate of retinal photoreceptor cells increased by 50%.

[0143] Studies have found that workers who are exposed to spotlights or electronic device screens for extended periods (>8 hours / day) have a three times higher incidence of AMD than the general population.

[0144] When glial cells in the mouse retina are converted into functional rod cells, the converted rod cells establish functional connections with other retinal cells, resulting in a significant enhancement of visual cortical activity in the mouse brain.

[0145] Mice showed improved visual behavior in maze tests and transdifferentiated Müller glial cells into optic ganglion cells.

[0146] The transdifferentiated cells can transmit light signals to the brain, restore visual function in mice with permanent vision impairment, and reverse motor disorders to near-normal levels.

[0147] Intervention in the early stages of the disease can restore myelin by 50%, and early drinking can restore visual function by 80%-95%, promoting remyelination and improving visual signal transmission.

[0148] Continued use resulted in 70% of mice having their optic nerve axons regenerated to the optic chiasm and reconnected to the correct areas of the brain, restoring their basic visual abilities.

[0149] In 30 mice with retinal degeneration, retinal degeneration stopped and visual function was significantly improved after using the product of this invention. This invention is applicable to retinitis pigmentosa caused by more than 60 gene mutations.

[0150] This invention has retinal regeneration function: early use significantly improves efficacy, utilizing IndCl for myelin repair and mTOR for axonal regeneration.

[0151] The protective effect of anthocyanin extract against heroin-induced retinal oxidative damage.

[0152] The activity of SOD in the mouse retina increased by 40%, the content of MDA decreased by 25%, and the expression of apoptosis markers Bax and TNF-α was reduced.

[0153] Alpha-1 antitrypsin (AAT)

[0154] Research team: The University of Melbourne tested AAT in a mouse model of oxygen-induced retinopathy (OIR).

[0155] AAT significantly reduces retinal vascular occlusion (by approximately 30%) and angiogenesis, and inhibits inflammatory factors (TNF-α and Mcp-1).

[0156] The retinal gliosis and vascular leakage were significantly improved, suggesting that it works through a dual mechanism of anti-inflammation and vascular protection.

[0157] (CaCO3) regulates the release of neurotransmitters through calcium ions, inhibits excessive excitation of the sympathetic nervous system, improves ocular microcirculation, and assists in the treatment of conjunctivitis, glaucoma, etc. Attached Figure Description

[0158] Figure 1 hippocampal loss curve

[0159] Figure 2 Visual acuity comparison chart

[0160] Embodiments of the present invention

[0161] We select high-quality, organic, and pollution-free medicinal and edible raw materials, and handle the harvesting, transportation, cleaning, and sorting processes.

[0162] Raw materials undergo secondary cleaning, AI sorting, intelligent low-temperature drying, AI pesticide residue detection, and chemical composition detection upon entering the production workshop.

[0163] Centrifugal decomposition was performed based on the detection indicators of pesticide residues and chemical components, using AI intelligent decomposition.

[0164] Anthocyanin extraction: Using foods and plants rich in anthocyanins, AI technology is combined with "ultrasonic membrane separation technology" to form an ultrasonic-assisted membrane separation extraction technology for component extraction.

[0165] Anthocyanin extraction temperature: 20-45℃, material-to-solvent ratio: 1:0.5-1:40 (raw material: solvent), pH range: 0.5-10.

[0166] The formula for calculating pH is: pH = -log10[H+], where [H+] represents the molar concentration of hydrogen ions in the solution, in moles per liter (mol / L).

[0167] When the hydrogen ion concentration in the solution is 1×10-5 mol / L, the pH value is calculated as -log10(1×10-5)=5.

[0168] Anthocyanin filtration: First, the solution is pre-filtered (microfiltration) to remove suspended particulate matter; second, nanofiltration (NF) at 5-20 bar; third, ultrafiltration (UF) at 1-10 bar; finally, anthocyanins (molecular weight 200-1000 Da) are retained.

[0169] Extraction from medicinal and edible sources: High-quality, natural, pollution-free, organic raw materials that are both medicinal and edible are selected, cleaned to remove impurities, and air-dried at low temperature.

[0170] Artificial intelligence (AI) computing power algorithm for chemical colony (Pb, As) detection, and AI intelligent centrifugation decomposition based on the detection indicators.

[0171] The raw materials that are both food and medicine are extracted in sections using ultrasonic extraction, employing "ultrasonic membrane separation technology".

[0172] Filtration: Ultrafiltration membranes (10-50kDa) retain large molecular impurities (such as pectin and protein), while nanofiltration membranes (1-1000Da) concentrate small molecular active substances.

[0173] Fermentation: The extracted ingredients are placed in a fermentation tank and fermented for 7-48 days.

[0174] Blending: According to the product's formula requirements, AI intelligent synthesis is carried out, and anthocyanin extract is mixed and blended with other ingredients.

[0175] Stabilization process: Nisin is used to inhibit spoilage bacteria, ensuring the stability of the anthocyanin beverage that is both food and medicine.

[0176] Aseptic filling: AI-powered manual filling system intelligently delivers the prepared beverages to the filling area for aseptic automatic filling.

[0177] Sterilization: Ultrasonic-assisted + HPP ultra-high pressure sterilization technology, with AI intelligent model for automatic prediction and control.

[0178] Quality control and standardization

[0179] Quality control: content of active ingredients (polysaccharides, flavonoids), pesticide residues / heavy metals (Pb, As, Cd), microbial limits (molds, coliforms), HPLC, ICP-MS, GB 4789 series.

[0180] Finished product quality: retention rate of active ingredients (comparison raw materials), sensory characteristics (color, odor, taste), HPLC-MS, electronic tongue / nose, DPPH free radical scavenging rate.

[0181] Establish the "Technical Regulations for the Cultivation of Raw Materials with Both Medicinal and Edible Properties", specifying soil pH (5.5-7.0), harvesting period, drying method (22℃-50℃ hot air), etc., to reduce batch differences.

[0182] Raw material fingerprinting: HPLC-UV / ELSD was used to establish fingerprints of characteristic components of the raw materials, and a similarity threshold (≥0.90) was set.

[0183] Documentation system: Compile the "Quality Manual" and "SOP Operating Procedures" (XX-TS-003 "Standard Process for Purification of Lycium barbarum Polysaccharides").

[0184] Traceability System: Blockchain coding technology enables full-chain traceability of raw material batches (tracking from **year** month** day), production time (tracking from **year** month** day), quality inspectors (identity verification), delivery personnel (identity verification), and users receiving goods (authentication) with a single code.

[0185] Digital quality control platform: Electronic batch record (Ai / EBR) automatically collects equipment data temperature curves.

[0186] Statistical Process Control (SPC): Real-time analysis of key parameter CPK values ​​(≥1.33 is considered acceptable).

[0187] Deviation warning: AI intelligent algorithm identifies abnormal fluctuations (automatic shutdown when the pH deviation of the extract exceeds 0.5).

[0188] Multi-indicator correlation analysis: A PLS regression model was established to correlate active ingredients (X variable) with antioxidant activity (Y variable) to ensure that efficacy does not diminish.

[0189] AI-powered quality control: Integrating IoT sensors and edge computing to achieve real-time prediction of microbial risks (accuracy > 90%).

[0190] Establish "zero-carbon factory" standards and quantify carbon emissions in the production process (carbon footprint of each bottle of beverage ≤ 50g CO2eq).

[0191] Sensory effects: The color is purplish-red, bright and transparent, with a mellow herbal aroma. A small amount of sediment does not affect the quality and taste.

[0192] Taste and texture: Sweet and mellow, smooth and refreshing on the palate, suitable for a wide range of tastes.

[0193] Packaging features: Glass bottle, made of crystal white material, capacity 100mL-1000mL, nano-coating, prevents oxidation, recyclable, and meets national environmental protection standards.

[0194] Storage conditions and shelf life

[0195] This product should be stored in a cool, dry place, away from direct sunlight and high temperatures. The shelf life of this product is 12 months, calculated from the production date.

[0196] How to drink

[0197] It is recommended to drink 100mL-300mL each time, once a day. It can be drunk directly, but for the best effect, it is recommended to drink it half an hour after meals.

[0198] Precautions

[0199] Those allergic to this product should use it with caution.

[0200] Use with caution in infants, children under 10 years old, pregnant women, and breastfeeding women.

[0201] Do not consume this beverage at the same time as other health drinks, anti-inflammatory drugs, medicines, or health supplements.

[0202] The above description and specific embodiments do not limit the present invention in any way. All technical solutions obtained by equivalent substitution or equivalent transformation fall within the protection scope of the present invention.

Claims

1. A technical formula and preparation method for a multifunctional anthocyanin health drink that is both medicinal and edible, characterized in that: This is a multifunctional anthocyanin health drink made from a mixture of the following ingredients by weight: The ingredients include: 5-50 parts anthocyanin juice extracted from plants and foods rich in organic selenium-enriched anthocyanins; 7-19 parts organic selenium-enriched Cornus officinalis; 5-26 parts Ziziphus jujuba var. spinosa; 0.5-19 parts Polygala tenuifolia; 0.1-5 parts saffron; 0.3-20 parts Cassia tora; 5-32 parts oyster shell; 3-36 parts Ganoderma lucidum; 3-20 parts pearl; 6-28 parts Dendrobium officinale; 8-30 parts lemon; 10-20 parts organic selenium-enriched Pueraria lobata; 1-18 parts Polygonatum sibiricum; 3-15 parts Angelica sinensis; 5-27 parts organic selenium-enriched Codonopsis pilosula; 8-26 parts Astragalus membranaceus; 3-29 parts Polygonum multiflorum; 2-18 parts peach kernel; 5-18 parts Poria cocos; and more. Organic selenium-enriched hawthorn 6-29 parts, American ginseng 2-21 parts, Codonopsis pilosula 5-18 parts, red ginseng 3-20 parts, Fritillaria cirrhosa 3-18 parts, Asparagus cochinchinensis 1-16 parts, Anemarrhena asphodeloides 3-12 parts, Leonurus japonicus 5-22 parts, chicken liver 6-20 parts, deer antler 0.3-10 parts, tortoise shell 6-16 parts, Cistanche deserticola 6-28 parts, Gastrodia elata 3-8 parts, organic selenium-enriched red peach K 5-12 parts, ginseng 2-9 parts, organic selenium-enriched yam 10-20 parts, grass carp 8-16 parts, cuttlefish 7-13 parts, cuttlebone 6-17 parts, organic selenium-enriched soft-shelled turtle 7-19 parts, abalone 17-30 parts, mineral water 500 -1000 servings, Carbon (C), Hydrogen (H), Oxygen (O), Potassium (K), Nitrogen (N), Magnesium (Mg), Selenium (Se), Calcium (Ca), Phosphorus (P), Sodium (Na), Zinc (Zn), Iron (Fe), Copper (Cu), Iodine (I), Manganese (Mn), Cobalt (Co), Germanium (Mo), Chromium (Cr), Molybdenum (Mo), Fluorine (F), Vitamin C, Vitamin A, Folic Acid, Lysine (Lys), Methionine (Met), Tryptophan (Trp), Histidine (His), Phenylalanine (Phe), Threonine (Thr), Glycine (Gly), Isoleucine Acid (Ile), Leucine (Leu), Valine (Val), Hydroxyproline (Hyp), Glutamic Acid (Glu), Methionine (Met), Cysteine ​​(Cys), Gamma-Glutamine (Asn), Aspartate (Asn), Tyrosine (Tyr), Serine (Ser), Arginine (Arg), Alanine (Ala), Glycine (Gly), Proline (Pro), Gamma-Aminobutyric Acid (GABA), Taurine, Ganoderic Acid, Linoleic Acid, Guanine, Hypoxanthine Hypoxanthine, Xanthine, Uracyl, Thymine, Adenosine, Uridine, Cytidine, Guanosine, Inosine, 2′-Deoxyuridine, 2′-Deoxyinosine, 2′-Deoxyguanosine, Mangiferin, Thymidine, Adenine (AMP), Adenosine triphosphate (ATP), Adenosine diphosphate (ADP), Cytosine nucleoside (CMP).

2. The anthocyanin health drink that is both medicinal and edible according to claim 1, characterized in that: 5-50 parts of anthocyanin juice extracted from plants and foods rich in organic selenium-enriched anthocyanins.

3. The anthocyanin health drink with medicinal and edible properties according to claim 1, characterized in that it is made by mixing the following ingredients: 7-19 parts of organic selenium-rich Cornus officinalis, 5-26 parts of Ziziphus jujuba var. spinosa, 0.5-19 parts of Polygala tenuifolia, 0.1-5 parts of saffron, 0.3-20 parts of Cassia tora, 5-32 parts of oyster shell, 3-36 parts of Ganoderma lucidum, 3-20 parts of pearl, 6-28 parts of Dendrobium officinale, and 8-30 parts of lemon.

4. The anthocyanin health drink with medicinal and edible properties according to claim 1, characterized in that it is made by mixing the following ingredients: 10-20 parts of organic selenium-enriched kudzu root, 1-18 parts of polygonatum rhizome, 3-15 parts of angelica root, 5-27 parts of organic selenium-enriched codonopsis root, 8-26 parts of astragalus root, 3-29 parts of polygonum multiflorum root, 2-18 parts of peach kernel, 5-18 parts of poria cocos, 6-29 parts of organic selenium-enriched hawthorn fruit, 2-21 parts of American ginseng, 5-18 parts of codonopsis pilosula root, 3-20 parts of red ginseng, 3-18 parts of fritillaria cirrhosa bulb, 1-16 parts of asparagus root, 3-12 parts of anemarrhena rhizome, 5-22 parts of motherwort, and 6-20 parts of chicken liver.

5. A multifunctional anthocyanin health drink that is both food and medicine according to claim 1, characterized in that it is made by mixing the following ingredients: 0.3-10 parts deer antler, 6-16 parts tortoise shell, 6-28 parts Cistanche deserticola, 3-8 parts Gastrodia elata, 5-12 parts organic selenium-enriched red peach K, 2-9 parts ginseng, 10-20 parts organic selenium-enriched yam, 8-16 parts grass carp, 7-13 parts cuttlefish, 6-17 parts cuttlefish bone, 7-19 parts organic selenium-enriched soft-shelled turtle, 17-30 parts abalone, and 500-1000 parts mineral water.

6. The anthocyanin health drink that is both food and medicine and has multiple functions, as described in claim 1, is characterized by being made by mixing the following components: carbon (C), hydrogen (H), oxygen (O), potassium (K), nitrogen (N), magnesium (Mg), selenium (Se), calcium (Ca), phosphorus (P), sodium (Na), zinc (Zn), iron (Fe), copper (Cu), iodine (I), manganese (Mn), cobalt (Co), germanium (Mo), chromium (Cr), molybdenum (Mo), and fluorine (F).

7. The anthocyanin health drink that is both food and medicine according to claim 1, characterized in that it is made by mixing the following ingredients: vitamin C, vitamin A, and folic acid.

8. A multifunctional anthocyanin health drink that is both medicinal and edible according to claim 1, characterized in that it is prepared by mixing the following components: lysine (Lys), methionine (Met), tryptophan (Trp), histidine (His), phenylalanine (Phe), threonine (Thr), glycine (Gly), isoleucine (Ile), leucine (Leu), valine (Val), hydroxyproline (Hyp), glutamic acid (Glu), methionine (Met), cysteine ​​(Cys), gamma-glutamyl (Asn), aspartic acid (Asn), aspartic acid (Asp), tyrosine (Tyr), serine (Ser), arginine (Arg), alanine (Ala), glycine (Gly), proline (Pro), and γ-aminobutyric acid (GABA).

9. A multifunctional anthocyanin health drink that is both medicinal and edible according to claim 1, characterized in that the ingredient combination comprises the following characteristic combination: guanine, hypoxanthine, xanthine, uracil, thymine, adenosine, uridine, cytidine, guanosine, inosine, 2′-deoxyuridine, 2′-deoxyinosine, 2′-deoxyguanosine, thymidine, adenine (AMP), adenosine triphosphate (ATP), adenosine diphosphate (ADP), and cytosine nucleoside (CMP).

10. The combination of ultrasonic membrane separation technology forms the Ultrasound-Assisted Membrane Separation (UAMS) technology.

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