South medicine compound preparation for preventing and treating porcine diarrhea as well as preparation method and application of south medicine compound preparation

Abstract

The invention discloses a traditional Chinese medicine composition which comprises the following components in parts by weight: 60-80 parts of pithecellobium clypearia, 4-11 parts of jasminum elongatum, 4-11 parts of Chinese knotweed herb, 4-11 parts of ovateleaf holly bark, 2-8 parts of gardenia, 1-6 parts of radix sophorae flavescentis, 1-3 parts of poria cocos and 1-6 parts of citrus chachiensis hortorum. The traditional Chinese medicine composition takes south medicine as a formula component, can replace antibiotics widely used in the pig breeding industry, realizes prevention and / or treatment of pig diarrhea, can improve the pig performance of pigs, including pig daily gain and feed conversion rate, also can improve the natural resistance of the pigs, reduces the occurrence of diseases, and improves the immunity of the pigs. The dependence of pigs on antibiotics is further reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine compositions, in particular, it relates to a south medicine compound preparation for preventing and treating pig diarrhea and a preparation method and application thereof. BACKGROUND

[0002] The long-term and large-scale use of antibiotics in the livestock and poultry industry in China has brought economic effects to the industry development, but also has caused many drawbacks. Although antibiotics have the effects of preventing diseases, promoting growth and improving the performance of livestock and poultry products, the long-term and large-scale unreasonable use in animal breeding industry has caused problems such as antibiotic residues and bacterial resistance, which seriously endanger the healthy and sustainable development of the livestock industry. In addition, antibiotics can also enter the human body through biological enrichment and then endanger human health. In recent years, the drug residues and drug resistance caused by the improper use of antibiotics have attracted widespread attention. The "ban on antibiotics" at the feed end, "reduction of antibiotics" and "limitation of antibiotics" at the breeding end, and "antibiotic-free" at the product end have become an inevitable trend of the development of the livestock-related industry.

[0003] However, the comprehensive "ban on antibiotics" at the feed end may reduce the production level of the breeding industry and increase the breeding cost. The abuse of antibiotics in the pig breeding process can damage the immune program of pigs, increase the number of drug-resistant microbial flora, cause imbalance of the microbial flora of pigs, and thus cause digestive system problems.

[0004] Compared with traditional Western medicine, modern Chinese medicine preparations have many advantages. They are mainly made of pure natural herbal plants, have the advantages of abundant resources, various varieties, good curative effect, good quality and low price, wide application, low safety and toxicity, almost no residues, no drug resistance and no serious environmental pollution, and can effectively solve the problem of antibiotic resistance in livestock products, and have the dual functions of nutrition and medicine. Under the promotion of the "antibiotic-free" policy, the use of Chinese herbal medicine to make additives has become a new trend of "reduction of antibiotics" and "replacement of antibiotics".

[0005] As an important branch of traditional Chinese medicine, south medicine refers to the medicinal materials produced in all or most of the provinces south of the Yangtze River and north of the Nanling Mountains, mainly including Jiang Zhikou, Jian Zexie, realgar, Shihua, Xionghuang, Nansha Shen, Zhizi, Baifeng, Huotanmu, Qutuofeng, Chexucao, Jiubijiang, Shiliupi, etc. Traditional Chinese medicine has been widely used in the breeding industry due to its antibacterial and anti-inflammatory effects and the ability to improve immunity.

[0006] At present, natural plant Chinese herbal medicine is mainly used as a feed additive to replace antibiotics. These additives aim to prevent and treat diseases, promote growth, and have no side effects and drug resistance, which are economic and environmentally friendly. However, as a genuine medicinal material, south medicine has not been used in the prevention and treatment of weaned piglet diarrhea with a mature prescription.

[0007] Therefore, there is a need for a traditional Chinese medicine composition composed of southern medicinal herbs to achieve the prevention and treatment of pig diarrhea using southern medicinal herbs. SUMMARY

[0008] The present application aims to overcome the above-mentioned deficiencies of the prior art and provide a southern medicinal herb composite preparation for preventing and treating pig diarrhea, a preparation method thereof, and applications thereof.

[0009] The first object of the present application is to provide a traditional Chinese medicine composition.

[0010] The second object of the present application is to provide the use of the above-mentioned traditional Chinese medicine composition in the preparation of a medicine for preventing and / or treating pig diarrhea.

[0011] The third object of the present application is to provide the use of the above-mentioned traditional Chinese medicine composition in reducing the feed conversion ratio of pigs.

[0012] The fourth object of the present application is to provide the use of the above-mentioned traditional Chinese medicine composition in improving the immunity of pigs.

[0013] The fifth object of the present application is to provide a preparation method of a southern medicinal herb composite preparation for preventing and treating pig diarrhea.

[0014] The sixth object of the present application is to provide a southern medicinal herb composite preparation prepared by the above-mentioned preparation method.

[0015] The seventh object of the present application is to provide a method for reducing the feed conversion ratio of pigs.

[0016] To achieve the above-mentioned objects, the present application is implemented by the following solutions:

[0017] A traditional Chinese medicine composition, comprising the following ingredients in parts by weight:

[0018] 60-80 parts of Adansonia digitata, 4-11 parts of Entada phaseoloides, 4-11 parts of Rubus corchorifolius, 4-11 parts of Rhododendron ovatum, 2-8 parts of Gardenia jasminoides, 1-6 parts of Sophora flavescens, 1-3 parts of Poria cocos, and 1-6 parts of Citrus grandis.

[0019] Preferably, the composition comprises the following ingredients in parts by weight:

[0020] 80 parts of Adansonia digitata, 4.5 parts of Entada phaseoloides, 4.5 parts of Rubus corchorifolius, 4.5 parts of Rhododendron ovatum, 2.7 parts of Gardenia jasminoides, 1.4 parts of Sophora flavescens, 1 part of Poria cocos, and 1.4 parts of Citrus grandis.

[0021] The traditional Chinese medicine composition of the present application uses southern medicinal herbs as formula components, which can replace the widely used antibiotics in pig breeding industry, achieve the prevention and / or treatment of pig diarrhea, improve the growth performance of pigs (including daily weight gain and feed conversion rate), and also improve the natural resistance of pigs, reduce the occurrence of diseases, and thus reduce the dependence of pigs on antibiotics.

[0022] Therefore the present application also claims the use of any of the above-mentioned traditional Chinese medicine compositions in the preparation of a medicine for preventing and / or treating pig diarrhea.

[0023] Preferably, the pig is a weaned piglet.

[0024] More preferably, the medicine further comprises a pharmaceutically acceptable excipient.

[0025] The present application also claims the use of any of the above-mentioned traditional Chinese medicine compositions in reducing the feed conversion ratio of pigs.

[0026] The present application also claims the use of any of the above-mentioned traditional Chinese medicine compositions in increasing the daily weight gain of pigs.

[0027] The present application also claims the use of any of the above-mentioned traditional Chinese medicine compositions in increasing the immunity of pigs.

[0028] The present application also claims a preparation method of a south medicine compound preparation for preventing and treating pig diarrhea, comprising the following steps:

[0029] S1. mixing any of the above-mentioned traditional Chinese medicine compositions with water, fully decocting, and then separating the solid and liquid, and collecting the liquid;

[0030] S2. concentrating the liquid obtained in step S1 to a crude drug concentration of 0.2 g / mL-2 g / mL to obtain the medicine.

[0031] Preferably, the fully decocting in step S1 specifically comprises the following steps:

[0032] S11. mixing any of the above-mentioned traditional Chinese medicine compositions with water according to a mass ratio of 1:6-10, boiling for 1-1.5 h, and then separating the solid and liquid to obtain the dregs and the liquid 1;

[0033] S12. mixing the dregs obtained in step S11 with water according to a mass ratio of 1:2-4, boiling for 0.5-1 h, and then separating the solid and liquid to obtain the liquid 2;

[0034] S13. combining the liquid 1 obtained in step S11 and the liquid 2 obtained in step S12.

[0035] Preferably, the concentration in step S2 is to a crude drug concentration of 1 g / mL.

[0036] Preferably, the pig is a weaned piglet.

[0037] The present application also claims the south medicine compound preparation prepared by any of the above-mentioned preparation methods.

[0038] The present application also claims a method for reducing the feed conversion ratio of pigs, which utilizes feed containing the above-mentioned south medicine compound preparation with a final mass concentration of 2.5%-5%.

[0039] Preferably, the feed containing the Nan medicine compound preparation with a mass final concentration of 5% is used for feeding.

[0040] Compared with the prior art, the application has the following beneficial effects:

[0041] The application provides a traditional Chinese medicine composition, which contains the following components in parts by weight: 60-80 parts of Iodes cruciata, 4-11 parts of Tiliacora pallida, 4-11 parts of Rubus corchorifolius, 4-11 parts of Rhodotypos umbellata, 2-8 parts of Gardenia jasminoides, 1-6 parts of Sophora flavescens, 1-3 parts of Poria cocos and 1-6 parts of Citrus grandis. The traditional Chinese medicine composition uses Nan medicine as a formula component, can replace the antibiotics widely used in the pig breeding industry, and has the following advantages: (1) replacing the antibiotics widely abused in the pig breeding industry, reducing and eliminating the problems of drug resistance and drug residue; (2) improving the pig production performance; (3) enhancing the pig immunity; (4) reducing the environmental pollution; (5) improving the food safety; (6) promoting the healthy breeding; (7) providing a new economic growth point; and (8) promoting the modern application of traditional Chinese medicine. BRIEF DESCRIPTION OF DRAWINGS

[0042] Figure 1 It is a histopathological detection result diagram of each piglet in Example 3; A is the duodenum villus length of each group of piglets, B is the jejunum villus length of each group of piglets, C is the ileum villus length of each group of piglets, D is the duodenum crypt depth of each group of piglets, E is the jejunum crypt depth of each group of piglets, and F is the ileum crypt depth of each group of piglets;

[0043] Figure 2 It is a result diagram of the organ index of each group of piglets in Example 3;

[0044] Figure 3 It is a result diagram of the immune level detection of each group of piglets in Example 3; A is the IgA expression level of each group of piglets, B is the IgN expression level of each group of piglets, C is the IgG expression level of each group of piglets, D is the IL-6 expression level of each group of piglets, E is the IL-2 expression level of each group of piglets, F is the IL-17 expression level of each group of piglets, and G is the TNF-α expression level of each group of piglets;

[0045] Figure 4The expression detection results of tight junction proteins and JAK2 / STAT3 pathway in the jejunum of each group of piglets in Example 3 are shown in the figure; A is a Western blotting figure of STAT3 protein and JAK2 protein, A-a is a protein relative expression level result figure of p-STAT3, A-b is a protein relative expression level result figure of p-JAK2, B is a Western blotting figure of ZO-1 protein, Occludin protein and Claudin-1 protein, B-a is a protein relative expression level result figure of ZO-1, B-b is a protein relative expression level result figure of Occludin, B-c is a protein relative expression level result figure of Claudin-1, C is a mRNA relative expression level result figure of ZO-1, D is a mRNA relative expression level result figure of Claudin-1, E is a mRNA relative expression level result figure of Occludin, F is a mRNA relative expression level result figure of STAT3, G is a mRNA relative expression level result figure of JAK2, H is a mRNA relative expression level result figure of IL-6. DETAILED DESCRIPTION

[0046] The application will be further described in conjunction with the drawings of the specification and specific examples, which are used to explain the application and are not used to limit the scope of the application. The test methods used in the following examples are conventional methods unless otherwise specified, and the materials, reagents, etc. used are commercially available reagents and materials unless otherwise specified.

[0047] The animal experiments involved in the examples of the application are approved by the ethics committee;

[0048] The feed for feeding piglets in the examples of the application is fresh piglet compound feed, which is purchased from Heilongjiang Province Sanhe Feed Co., Ltd., and the item number is LM300.

[0049] Example 1 Preparation of the south medicine composition liquid

[0050] I. South medicine composition formula

[0051] The component formula of the south medicine composition is shown in Table 1.

[0052] Table 1 Component formula of the south medicine composition

[0053] South medicine components Monkeys ear ring Twisted body Fire mother Savior Gardenia Sophora Poria cocos Broad pericarp Content / g 80 4.5 4.5 4.5 2.7 1.4 1 1.4

[0054] II. Preparation method

[0055] The preparation method of the south medicine composition includes the following steps:

[0056] S1. According to the component formula shown in Table 1, accurately weigh the corresponding mass of the south medicine components, mix them uniformly, then add 800 g of distilled water, boil and keep for 1 h, then separate the solid and liquid to obtain the residue and the liquid 1;

[0057] S2. Add 300 g of distilled water to the residue obtained in step S1, boil and keep for 0.5 h, repeat twice, then separate the solid and liquid to obtain the liquid 2;

[0058] S3. Combine the liquid 1 obtained in step S1 and the liquid 2 obtained in step S2, then fully stand still, place in a centrifuge at 2500 r / min for 10 min, and collect the supernatant;

[0059] S4. Filter the supernatant, collect the filtered supernatant, and place it in a 70℃ water bath to concentrate to a crude drug concentration of 1 g / mL, thereby obtaining the south medicine composition liquid.

[0060] Example 2: Effect of the south medicine composition on the growth and development of weaned piglets

[0061] I. Experimental method

[0062] 1. Experimental animal setting

[0063] Select 24 14-day-old piglets with similar body weight and in good health that are fed with antibiotic-free feed, all of which have been immunized with foot-and-mouth disease, swine fever and pseudorabies vaccines, and are raised in a strictly disinfected pen, and the piglets are free to eat and are fed with antibiotic-free feed and high-temperature sterilized drinking water.

[0064] 2. Preparation of the south medicine lyophilized powder

[0065] According to the method shown in Example 1, the south medicine composition liquid is prepared, and the south medicine composition liquid is placed in a vacuum freeze dryer to prepare the south medicine composition lyophilized powder.

[0066] 3. Experimental animal grouping and treatment

[0067] The 24 14-day-old piglets are randomly divided into 4 groups, with 6 piglets in each group, namely the control group, the low-dose south medicine group, the high-dose south medicine group and the antibiotic group (positive control group), and the treatments of each group are as follows:

[0068] Control group: the piglets are fed with feed according to the method of free feeding twice a day.

[0069] The only difference between the low-dose south medicine group and the control group is that the feed is replaced with feed mixed with 0.25% (w / w) south medicine composition lyophilized powder, and the rest of the treatment is exactly the same.

[0070] The only difference between the high-dose southern medicine group and the control group is that the feed is replaced with feed mixed with 0.5% (w / w) southern medicine composition freeze-dried powder, and the rest of the treatment is exactly the same.

[0071] The only difference between the antibiotic group and the control group is that the feed is replaced with feed mixed with 0.05% (w / w) soluble powder of neomycin sulfate, and the rest of the treatment is exactly the same.

[0072] The 14-day-old piglets in each group were continuously fed according to the corresponding treatment method until 35 days old, and the following indicators were calculated:

[0073] (1) Average weight gain: The piglets were weighed before and after the start of feeding, and the total weight gain and average weight gain of the piglets in each group during the feeding process were calculated;

[0074] (2) Diarrhea index: During the feeding process, the feces of piglets in each group were scored daily according to the feces scoring table shown in Table 2, and the diarrhea index of piglets in each group was calculated according to formula I;

[0075] Table 2 Feces Scoring Table

[0076] Degree Appearance Initial water content in feces Score Normal Bar or granular ＜70 0 Mild Soft stool, can be shaped 70～75 1 Moderate Thick, not shaped, fecal water without separation phenomenon 75～80 2 Severe Liquid, not shaped, fecal water with separation phenomenon ＞80 3

[0077] Formula I: Diarrhea index = (sum of diarrhea scores of all piglets in the group during feeding) / (feeding days * number of piglets in the group);

[0078] (3) Feed intake and feed conversion ratio: The feed intake of piglets in each group was recorded daily during the feeding process, and the total feed intake and average daily feed intake of piglets in each group were calculated, and the feed conversion ratio of piglets in each group was calculated according to formula II;

[0079] Formula II: Feed conversion ratio = (total feed intake of piglets in each group during feeding) / (total weight gain of piglets in each group during feeding);

[0080] II. Experimental Results

[0081] The growth performance results of piglets in each group are shown in Table 3, which includes average daily weight gain, average daily feed intake, feed conversion ratio and diarrhea index.

[0082] Table 3 Growth performance results of piglets in each group

[0083] Average daily weight gain / g Average daily intake / g Feed to meat ratio Diarrhea index Control group 196.67 192.76 0.98 0.022 Low-dose south medicine group 259.84 ab ]] 198.63 0.76 ab ]] 0.005 High-dose south medicine group 237.94 a ]] 194.42 0.82 a ]] 0.003 a ]] Antibiotic group 205.48 192.66 0.94 0.003

[0084] Note: The superscript "a" in the table indicates a significant difference compared with the control group, and "b" indicates a significant difference compared with the antibiotic group.

[0085] The results show that: compared with the control group, the average daily weight gain of piglets in the low-dose southern medicine group and the high-dose southern medicine group is significantly increased, and the feed-meat ratio is significantly reduced, and the diarrhea index is also significantly reduced, indicating that the southern medicine composition liquid prepared by example 1 can significantly reduce the diarrhea index of weaned piglets, and achieve obvious antidiarrheal effect.

[0086] At the same time, compared with the antibiotic group, the average daily weight gain of piglets in the low-dose southern medicine group and the high-dose southern medicine group is significantly higher, and the feed-meat ratio is also lower, indicating that compared with using antibiotics to feed weaned piglets, using the southern medicine composition liquid prepared by example 1 to feed weaned piglets can improve the growth performance of weaned piglets and improve the feed utilization rate.

[0087] Example 3 Effect of Southern Medicine Composition on Immune Function of Weaned Piglets

[0088] I. Experimental method

[0089] 1. Experimental animal setting

[0090] Select 24 14-day-old piglets with similar body weight and health that are fed with antibiotic-free feed. All piglets are immunized with foot-and-mouth disease, swine fever and pseudorabies vaccine, and are raised in a strictly disinfected pen. Piglets are free to eat and are fed with antibiotic-free feed and high-temperature sterilized drinking water.

[0091] 2. Preparation of southern medicine freeze-dried

[0092] According to the method shown in example 1, the southern medicine composition liquid is prepared, and the southern medicine composition liquid is placed in a vacuum freeze dryer to prepare a southern medicine composition freeze-dried powder.

[0093] 3. Experimental animal grouping and treatment

[0094] The 24 14-day-old piglets are randomly divided into 4 groups, 6 piglets in each group, namely control group, low-dose southern medicine group, high-dose southern medicine group and antibiotic group (positive control group). The treatment of each group is as follows:

[0095] Control group: use feed to feed piglets according to the method of free feeding twice a day.

[0096] The only difference between the low-dose southern medicine group and the control group is that the feed is replaced with feed mixed with 0.25% (w / w) southern medicine composition freeze-dried powder, and the rest of the treatment is exactly the same.

[0097] The only difference between the high-dose southern medicine group and the control group is that the feed is replaced with feed mixed with 0.5% (w / w) southern medicine composition freeze-dried powder, and the rest of the treatment is exactly the same.

[0098] The only difference between the antibiotic group and the control group was that the feed was replaced with 0.05% (w / w) neomycin sulfate soluble powder, and the rest of the treatment was exactly the same.

[0099] The 14-day-old piglets in each group were continuously fed according to the corresponding treatment method until 35 days old, and then the piglets in each group were euthanized, the intestinal histopathology was examined, the duodenal villus length and crypt depth, the jejunum villus length and crypt depth, and the ileum villus length and crypt depth of the piglets were measured; and the heart weight, liver weight, kidney weight and spleen weight of the piglets were measured, and the organ weight to body weight ratio was calculated to obtain the organ index, including heart index, liver index, kidney index and spleen index.

[0100] The levels of IgA, IgM, IgG, IL-6, IL-2, IL-17 and TNF-α in the piglets in each group were detected using ELISA kits from Shanghai Enzyme-Linked Biotechnology (model numbers: MM-090501, MM-040301, MM-038301, MM-042101, MM-041801, MM-040201, MM-7784001) according to the kit instructions.

[0101] Next, the jejunum protein of the piglets in each group was extracted, the protein was separated by SDS-PAGE and transferred to a 0.45 μm polyvinylidene fluoride (PVDF) membrane, after blocking with Tris buffer saline solution (TBST) containing 5% (w / w) skimmed milk powder at 25°C for 1 hour, then incubated with primary antibody (primary antibody as shown in Table 4) at 4°C for 16 hours, washed with TBST, then incubated with HRP-conjugated secondary antibody (1:5000, v / v) (Kangwei Century, CW0103S / CW0102S) at 25°C for 1 hour, then detected the protein bands using an enhanced chemiluminescence detection (ECL) kit, and analyzed using Image J (version 1.51j8) software to obtain the expression of tight junction proteins and JAK2 / STAT3 pathway in the jejunum of piglets in each group.

[0102] Table 4 Primary antibody information

[0103] Antibody Brand Part number Jak2 Cell signaling 3230T P-Stat3 Cell signaling 9145T P-Jak2 Cell signaling 4406T Claudin 1 PROTEINTECH 13050-1-AP Occludin PROTEINTECH 13409-1-AP ZO-1 PROTEINTECH 21773-1-AP STAT3 ABClonal A1192 GAPDH bioworld MB001 Β-actin Abmart M20011

[0104] The mRNA of each group of piglets was extracted, and the extracted mRNA was reverse transcribed into cDNA using HiScript III RT SuperMix for quantitative PCR kit (Vazyme, China) according to its instructions; the qPCR amplification upstream and downstream primers of Occludin, ZO-1, Claudin-1, β-actin, IL-6, STAT3 and JAK2 were designed based on NCBI GeneBank, as shown in Table 5.

[0105] Table 5 qPCR amplification upstream and downstream primers

[0106]

[0107] 1 μL of cDNA was used to perform real-time fluorescent PCR amplification on a real-time fluorescent quantitative PCR instrument (LightCycler480, Roche, USA) using ChamQ Universal SYBR quantitative PCR Master Mix (Vazyme, China) combined with the qPCR amplification upstream and downstream primers shown in Table 5, to obtain the relative expression level of mRNA of STAT3 protein, JAK2 protein, ZO-1 protein, Occludin protein, Claudin-1 protein and IL-6 protein.

[0108] II. Experimental results

[0109] The histopathological detection results of each group of piglets are shown in Figure 1 A is the duodenal villus length of each group of piglets, B is the jejunum villus length of each group of piglets, C is the ileum villus length of each group of piglets, D is the duodenal crypt depth of each group of piglets, E is the jejunum crypt depth of each group of piglets, and F is the ileum crypt depth of each group of piglets.

[0110] The results show that: compared with the control group, whether it is the low-dose Nan medicine group or the high-dose Nan medicine group, the villus length and crypt depth of the duodenum, jejunum and ileum of weaned piglets are significantly increased, and the high-dose Nan medicine group is more obvious; at the same time, compared with the antibiotic group, the villus length and crypt depth of the duodenum, jejunum and ileum of weaned piglets in the low-dose Nan medicine group and the high-dose Nan medicine group are also significantly increased.

[0111] It is shown that using the Nan medicine composition liquid prepared in Example 1 to feed weaned piglets can enhance the absorption efficiency of nutrients by weaned piglets and promote the intestinal health of weaned piglets.

[0112] The organ index results of each group of piglets are shown in Figure 2As shown in the figure, the results show that there is no significant difference in the heart index, liver index, kidney index and spleen index of piglets in the control group, low-dose southern medicine group, high-dose southern medicine group and antibiotic group, indicating that feeding weaned piglets with feed containing the southern medicine composition liquid prepared in Example 1 will not affect the growth and development of the organs of weaned piglets.

[0113] The detection results of the immune levels of piglets in each group are shown in the figure Figure 3 As shown in the figure, A is the IgA expression level of piglets in each group, B is the IgN expression level of piglets in each group, C is the IgG expression level of piglets in each group, D is the IL-6 expression level of piglets in each group, E is the IL-2 expression level of piglets in each group, F is the IL-17 expression level of piglets in each group, and G is the TNF-α expression level of piglets in each group.

[0114] The results show that compared with the control group, the IgA, IgM and IgG expression levels of piglets in the high-dose southern medicine group are significantly increased, and the IL-6, IL-2, IL-17 and TNF-α expression levels are significantly reduced; the IgM, IL-6 and TNF-α expression levels of piglets in the low-dose southern medicine group have no significant change compared with the control group, but other changes are obvious; and the IgA, IgM and IgG expression levels of piglets in the antibiotic group are even significantly reduced compared with the control group.

[0115] It is indicated that feeding weaned piglets with feed containing 5% (w / w) of the feed can improve the immunity of weaned piglets and enhance the resistance of weaned piglets to the outside world.

[0116] The detection results of the expression of tight junction proteins and JAK2 / STAT3 pathway in the jejunum of piglets in each group are shown in the figure Figure 4 As shown in the figure, A is the Western blotting figure of STAT3 protein and JAK2 protein, A-a is the relative protein expression level result figure of p-STAT3, A-b is the relative protein expression level result figure of p-JAK2, B is the Western blotting figure of ZO-1 protein, Occludin protein and Claudin-1 protein, B-a is the relative protein expression level result figure of ZO-1, B-b is the relative protein expression level result figure of Occludin, B-c is the relative protein expression level result figure of Claudin-1, C is the mRNA relative expression level result figure of ZO-1, D is the mRNA relative expression level result figure of Claudin-1, E is the mRNA relative expression level result figure of Occludin, F is the mRNA relative expression level result figure of STAT3, G is the mRNA relative expression level result figure of JAK2, and H is the mRNA relative expression level result figure of IL-6.

[0117] The results show that compared with the control group, the protein and mRNA expression levels of P-STAT3 / STAT3 and P-JAK2 / JAK2 in the low-dose southern medicine group and the high-dose southern medicine group are significantly down-regulated, and the protein and mRNA expression levels of ZO-1, Occludin and Claudin-1 are significantly up-regulated.

[0118] It is illustrated that feeding weaned piglets with feed mixed with 5% (w / w) of the feed can effectively control the intestinal inflammation of weaned piglets and protect the barrier function of the intestinal tract.

[0119] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application, and are not intended to limit the scope of protection of the present application. For those skilled in the art, on the basis of the above description and ideas, other different forms of changes or modifications can also be made, which do not need to be or cannot be exhaustively listed here. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection scope of the claims of the present application.

Claims

1. A traditional Chinese medicine composition, characterized in that, By weight parts, containing the following ingredients: 60-80 parts of Iodes fructus, 4-11 parts of Tinospora crispa, 4-11 parts of Rubus corchorifolius, 4-11 parts of Rabdosia rubescens, 2-8 parts of Gardenia jasminoides, 1-6 parts of Sophora flavescens, 1-3 parts of Poria cocos and 1-6 parts of Citrus grandis.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, By weight parts, containing the following ingredients: 80 parts of Iodes fructus, 4.5 parts of Tinospora crispa, 4.5 parts of Rubus corchorifolius, 4.5 parts of Rabdosia rubescens, 2.7 parts of Gardenia jasminoides, 1.4 parts of Sophora flavescens, 1 part of Poria cocos and 1.4 parts of Citrus grandis.

3. The use of the traditional Chinese medicine composition of any one of claims 1-2 in the preparation of a medicament for preventing and / or treating pig diarrhea.

4. Use according to claim 3, characterized in that, The pig is a weaned piglet.

5. The use of the traditional Chinese medicine composition of any one of claims 1-2 in reducing the feed conversion ratio of pigs.

6. The use of the traditional Chinese medicine composition of any one of claims 1-2 in improving the immunity of pigs.

7. A preparation method of a Chinese medicine compound preparation for preventing and treating pig diarrhea, characterized in that, The method comprises the following steps: S1. Mixing the traditional Chinese medicine composition of any one of claims 1-2 with water, fully decocting, and then separating the liquid from the solid, and collecting the liquid; S2. Concentrating the liquid obtained in step S1 to a crude drug concentration of 0.5 g / mL-2 g / mL to obtain the south medicine composite preparation.

8. The preparation method according to claim 7, characterized in that, In step S2, the concentration is to a crude drug concentration of 1 g / mL.

9. The south medicine composite preparation prepared by the preparation method of any one of claims 7-8.

10. A method of reducing the feed conversion ratio of pigs, characterized in that, The feed contains the south medicine composite preparation of claim 9 with a final concentration of 2.5%-5% by mass.

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