CO2 sustained-release capsule and preparation method thereof
By preparing CO2 sustained-release capsules containing sodium alginate, corn starch, amylase, and brewer's yeast, the problem of easy volatilization of inorganic CO2 sources is solved by utilizing the respiration of brewer's yeast and the action of amylase. This enables continuous attraction and elimination of underground pests, reducing the risk of environmental pollution.
Patent Information
- Application Number
- CN202511179331.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-22
- Publication Date
- 2025-11-14
AI Technical Summary
Existing inorganic CO2 sources are easily volatile and cannot effectively attract and disinfect underground pests in the long term, resulting in poor control effects.
The CO2 sustained-release capsule is composed of sodium alginate, corn starch, amylase and brewer's yeast. It utilizes the respiration of brewer's yeast to continuously release CO2, and combined with the action of amylase, it forms a stable source of CO2 release.
It achieves continuous and stable CO2 release, improves the attraction effect on underground pests, extends their lifespan, and reduces the risk of environmental pollution.
Smart Images
Figure CN120937874A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of crop pest and disease control technology, and particularly relates to a CO2 sustained-release capsule and its preparation method. Background Technology
[0002] CO2 is a major product of root respiration in crops and diffuses well in soil. It serves as a key cue and signaling molecule for many underground pests to locate their host plant roots. Multiple studies have shown that CO2 has a good attraction effect on the larvae of several species, including the North China black grub beetle (Holotrichia oblita), the dark-colored grub beetle (Holotrichia parallela), the green-colored grub beetle (Anomalacorpulenta), and the western maize root borer (Diabrotica virgifera virgifera). It can be used as a novel attractant for underground pests and has great potential for future pest control. Currently, the method of attracting western maize root borer larvae by applying inorganic CO2 sources composed of ammonium bicarbonate and / or urea reduces the insect population density at the roots during crop growth and interferes with the process of pests locating their host plant roots. However, the overall control efficacy is poor, possibly because underground pests have long life cycles, and the highly volatile inorganic CO2 source cannot achieve long-term attraction for these pests. Summary of the Invention
[0003] Given the high volatility of CO2, this invention proposes a CO2 slow-release capsule that can attract underground pests and its preparation method. The prepared CO2 slow-release capsule can continuously attract underground pests from the infestation site to the vicinity of the capsule, while simultaneously combining with other control measures to centrally eliminate underground pests, which is convenient and quick.
[0004] This invention is achieved through the following technical solution: a CO2 sustained-release capsule and its preparation method, characterized in that it is composed of the following components in mass fractions: sodium alginate 2.00%–3.00%, corn starch 10.00%–30.00%, amyloglucosidase 1.0–3.0 U / g, and brewer's yeast 10.00%–30.00%, with ultrapure water as the solvent; wherein the brewer's yeast is Saccharomyces cerevisiae BNCC185415.
[0005] Furthermore, the reagents in the capsules are in the following proportions: sodium alginate 3.00%, corn starch 30.00%, amyloglucosidase content 1.0-3.0 U / g, and brewer's yeast 10.00%.
[0006] Compared with the prior art, the present invention has the following beneficial effects:
[0007] The CO2 sustained-release capsules prepared in this invention derive their attractant from the respiration of Saccharomyces cerevisiae. This differs from conventional volatile, unstable insect attractants that cannot spread long distances in soil. The CO2 sustained-release capsules offer the absolute advantages of continuous and stable release, long service life, and low cost. Furthermore, all components of the sustained-release capsules are readily available, easily degradable, and biocompatible, avoiding the environmental pollution drawbacks caused by traditional attractant preparation processes during production and application. Attached Figure Description
[0008] Figure 1 This is a diagram of the external appearance of the capsule described in this invention.
[0009] Figure 2 The diagram shows the CO2 sustained-release rate of the capsules described in Example 1 and Comparative Examples 1 and 2 of this invention.
[0010] Figure 3 This is a CO2 concentration gradient map established in soil by the capsule described in Example 1 of the present invention.
[0011] Figure 4 The capsule described in Example 1 of this invention has an attraction effect on grubs. Detailed Implementation
[0012] To facilitate understanding of the present invention, a more comprehensive description will be given below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention.
[0013] Example 1
[0014] A matrix solution containing 3.00% sodium alginate, 30.00% corn starch, 1.0 U / g amylase, and 10.00% Saccharomyces cerevisiae was prepared using ultrapure water as the solvent and stirred until homogeneous. The matrix solution was then added dropwise to a 0.50% CaCl2 solution using a peristaltic pump. The resulting capsules were kept in the CaCl2 solution for 20 minutes, passed through an 18-mesh sieve, and washed with ultrapure water to obtain CO2 sustained-release capsules.
[0015] The only difference between Comparative Example 1 and Example 1 is that the brewer's yeast component was removed, so that the capsule could not release CO2.
[0016] The only difference between Comparative Example 2 and Example 1 is that the amylase component was removed, so that the capsule could not release CO2.
[0017] The purpose of Comparative Examples 1 and 2 is to clarify whether brewer's yeast and amylase are necessary conditions for the capsule to produce CO2.
[0018] Experimental Example 1
[0019] Capsules of equal mass from Example 1, Comparative Example 1, and Comparative Example 2 were placed in 250 mL sealed gas washing bottles. The amount of CO2 produced by the three types of capsules within 1 hour was measured using a pump-type CO2 analyzer. The gas washing bottles were sealed with a stopper containing two tubing, which were connected to the inlet and outlet of the analyzer, respectively. The gas washing bottles were initially filled with ambient air, and the CO2 release was then measured at various time points. The release rate formula is as follows: (C1 is the CO2 concentration in the washing bottle at the end of the measurement, C0 is the initial concentration in the washing bottle, m is the weight of the capsule, and h is the measurement time). Results are as follows: Figure 2 As shown.
[0020] Figure 2 The results showed that: the capsule of Example 1 produced a large amount of CO2 at the beginning of the measurement, and then the amount of CO2 released gradually decreased, and the release rate showed an overall trend of first decreasing and then stabilizing; Comparative Example 1 did not contain brewer's yeast and could not release CO2 through yeast fermentation; Comparative Example 2 did not contain amylase, corn starch could not be broken down into monosaccharides, and brewer's yeast did not have glycogen that could be directly utilized, so it could not release CO2.
[0021] Experiment Example 2
[0022] 10g of the capsules prepared in Example 1 were placed at a depth of 10cm in the soil, and the CO2 concentration at different time points and distances from the capsules was measured using a pump-type CO2 analyzer. The results are as follows: Figure 3 As shown.
[0023] Figure 3 The results showed that the CO2 concentration gradient established by the capsules prepared in Example 1 in the soil showed a decreasing trend over time, with the CO2 concentration reaching its peak on the 8th day, reaching as high as 5340 ppm at 0 cm.
[0024] Experimental Example 3
[0025] Ten grams of the capsules prepared in Example 1 were buried 10 cm deep in the soil. Second-instar larvae of the dark-spotted scarab beetle were placed at parallel depths of 10 cm, 20 cm, and 30 cm from the capsules. The attraction effect of the capsules on the grubs under different distances was statistically analyzed. The entire experiment was conducted in darkness, with soil moisture maintained at approximately 20%. The crawling distance of the grubs towards the CO2 attractant was observed and measured at 24 h, 48 h, and 72 h after treatment. Each treatment was replicated in triplicate. The results are as follows: Figure 4 As shown.
[0026] Figure 4The results showed that when the grubs were 10 cm away from the capsule, the attraction effect remained good throughout the observation period. When the grubs were 20 cm or 30 cm away from the capsule, the attraction effect on the grubs was good after 72 hours.
[0027] The embodiments described above are merely illustrative of implementation methods of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.
Claims
1. A CO2 sustained-release capsule and its preparation method, characterized in that, It is composed of the following components by mass fraction: sodium alginate 2.00%~3.00%, corn starch 10.00%~30.00%, amyloglucosidase 1.0~3.0 U / g, and brewer's yeast 10.00%~30.00%, with ultrapure water as the solvent; the brewer's yeast is... Saccharomyces cerevisiae BNCC185415.
2. The CO2 sustained-release capsule and its preparation method as described in claim 1, characterized in that, The optimal mass ratio of each component is 3.00% sodium alginate, 30.00% corn starch, 1.0 U g-1 amyloglucosidase, and 10.00% brewer's yeast.
3. The CO2 sustained-release capsule and its preparation method as described in claims 1-2, characterized in that, The specific preparation method includes the following steps: (1) Weigh 3 g sodium alginate, 30 g corn starch, 1.0 U / g amylase and 10 g brewer's yeast using an analytical balance, place them in a beaker, add 57 mL ultrapure water, and stir well. (2) Add the matrix solution dropwise to a 0.50% CaCl2 solution using a peristaltic pump. After 20 min, pass the solution through a sieve (18 mesh), wash the capsules three times with ultrapure water to obtain CO2 sustained-release capsules, and store them at 4℃.
4. The CO2 sustained-release capsule and its preparation method as described in claim 3, characterized in that, The peristaltic pump operates at a speed of 1 rpm.