Cancer tissue slice sampling device for tumor immunoassay

Abstract

The invention relates to the technical field of cell tissue sampling, and discloses a cancer tissue slice sampling device for tumor immunoassay, the cancer tissue slice sampling device comprises a case, a wax coating constraint assembly, a slice shifting assembly, a slice linear driver, a slice cutter, a feeding linear motor and a lifting bracket, and the wax coating constraint assembly is located in the case. The invention particularly provides a cancer tissue slice sampling device for tumor immunoassay, which can stably position a wax coated sample and can take slices one by one after integral cutting.

Description

Technical Field

[0001] This invention relates to the field of cell and tissue sampling technology, specifically to a tumor immunoassay sampling device for cancer tissue sections. Background Technology

[0002] Cancer tissue section refers to the process in the field of tumor immunoassay where doctors cut paraffin-impregnated tissue samples into very thin slices and stain them for microscopic observation and study.

[0003] The main reason for the production of defective sections with unacceptable thickness in cancer tissue sections is that the cutting force of the microtome will impact the wax-coated sample. After multiple high-frequency cuts, the wax-coated sample in the fixture will be deformed or unstable in position.

[0004] Meanwhile, traditional tissue microtome machines use a slice-by-slice sampling process, meaning that after each slice of the wax-coated sample is cut, the slice is immediately removed before the next cut. After some slices are removed, the remaining wax-coated sample becomes thinner, resulting in poor positioning and clamping stability of the wax-coated sample. Summary of the Invention

[0005] In view of the above situation and to overcome the defects of the prior art, the present invention provides a cancer tissue section sampling device for tumor immunoassay. In order to solve the problem of deformation or unstable positioning of wax-coated samples caused by repeated high-frequency cutting, the present invention proposes a cancer tissue section sampling device for tumor immunoassay that can stably position wax-coated samples and can be cut as a whole and then sampled one by one.

[0006] The technical solution adopted by the present invention is as follows: a tumor tissue section sampling device for tumor immunoassay, comprising a chassis, a wax-coating constraint assembly, a section shifting assembly, a section linear actuator, a section cutting tool, a feed linear motor, and a support bracket. The wax-coating constraint assembly is located inside the chassis, the section shifting assembly is located inside the chassis, the section linear actuator is fixedly mounted on the top of the chassis, the section cutting tool is fixedly mounted on the moving end of the section linear actuator, the feed linear motor is fixedly mounted on the bottom of the chassis, and the support bracket is fixedly mounted on the moving end of the feed linear motor.

[0007] Furthermore, a left support column and a right support column are fixedly installed inside the chassis. The wax-coating constraint assembly includes a four-sided tray, a left guide rod, and a right guide rod. The four-sided tray is fixedly installed on the support bracket. The left guide rod is fixedly connected to the left support column, and the right guide rod is fixedly connected to the right support column. The four-sided tray is provided with a rod groove. The left guide rod is slidably connected to the rod groove, and the right guide rod is slidably connected to the rod groove. The left and right guide rods are fixedly connected. The connecting surfaces of the left and right guide rods coincide with the center surface of the slicing blade. The left and top sides of the four-sided tray are empty. The four-sided tray is used to place wax-coated samples.

[0008] Furthermore, the wax-coated constraint assembly also includes a wedge-shaped baffle, a reversing block, a reversing inclined rail, and a traction core rod. The traction core rod is slidably connected to the right guide rod. The reversing block is slidably connected to the left end of the traction core rod in a direction perpendicular to the axis of the right guide rod. The reversing inclined rail is fixedly installed inside the right guide rod. The reversing block is slidably connected to the reversing inclined rail. The wedge-shaped baffle is fixedly installed at the left end of the reversing block. The right end of the left guide rod is provided with a blocking and clearance groove adapted to the wedge-shaped baffle. The four-sided support box is provided with an oblique through-hole communicating with the rod groove.

[0009] Furthermore, the wax-coated constraint assembly also includes a blocking linear actuator and a blocking traction plate. The blocking linear actuator is fixedly connected to the right support column, the blocking traction plate is fixedly connected to the moving end of the blocking linear actuator, and the blocking traction plate is fixedly connected to the traction core rod.

[0010] Furthermore, the wax-coating constraint assembly also includes a left-side clamping plate, a clamping slide fork, and expansion pins. The left-side clamping plate is used to clamp the left side of the wax-coated sample. The slide fork is slidably connected to the left-side clamping plate in the front-back direction and is slidably connected to the four-sided tray in the front-back direction. The right side of the left-side clamping plate and the four-sided tray are provided with nail holes adapted to the expansion pins. The expansion pins are used to nail into the left and right ends of the wax-coated sample.

[0011] Furthermore, the left support column is provided with a displacement clearance groove, and the slice displacement assembly includes a pitch adjustment motor, a swing frame, a displacement linear actuator, a pressure control plate, a piston rod, a piston cylinder, a rigid suction cup, and a pressure control linear actuator. The pitch adjustment motor is fixedly connected to the left support column, the swing frame is fixedly mounted on the rotating end of the pitch adjustment motor, the displacement linear actuator is fixedly mounted on the swing frame, the pressure control plate is fixedly mounted on the moving end of the displacement linear actuator, the piston rod is fixedly connected to the pressure control plate, the piston cylinder is slidably connected to the piston rod, the rigid suction cup is fixedly connected to the piston cylinder, the pressure control linear actuator is fixedly mounted on the piston cylinder, and the moving end of the pressure control linear actuator is fixedly connected to the pressure control plate.

[0012] Furthermore, a display tray is detachably provided on the left side of the chassis.

[0013] Furthermore, a heat exchange channel is provided at the rear of the chassis, and a door is provided at the front of the chassis.

[0014] The beneficial effects achieved by the present invention using the above structure are as follows:

[0015] 1. The wax-coated sample is tightly connected to the four-sided tray, so that the cutting impact of the slicing tool can be buffered and transmitted through a medium, avoiding deformation of the wax-coated sample due to repeated high-frequency cutting, which would render the sample unusable for research.

[0016] 2. By combining the left-side clamping plate, clamping slide fork, expansion pin, and four-sided support box, after the sectioning blade cuts, some of the sectioned wax-coated samples and the unsectioned wax-coated samples are kept in place to maintain the positioning accuracy between the overall wax-coated sample and the wax-coated constraint components, further ensuring the controllability of the section thickness and reducing the risk of waste sections.

[0017] 3. By combining the use of wedge baffles, reversing blocks, reversing inclined rails, traction core rods, blocking linear actuators, and blocking traction discs, the remaining slices can be limited and blocked during the process of the slice shifting assembly removing the leftmost slice, thus preventing multiple slices from adhering to each other and being removed and placed into the slide pool at the same time, thereby losing their research usability.

[0018] 4. A heat exchange channel is provided at the rear of the cabinet door to facilitate the continuous input of cold air into the cabinet, so as to maintain the low temperature of the slicing blade and avoid the adverse effects of cutting heat. Attached Figure Description

[0019] Figure 1 This is a three-dimensional schematic diagram of a tumor tissue section sampling device for tumor immunoassay according to the present invention.

[0020] Figure 2 This is a three-dimensional schematic diagram from another perspective of a tumor immunoassay sampling device for cancer tissue sections according to the present invention.

[0021] Figure 3 This is a three-dimensional schematic diagram of the wax-coated constraint component of the present invention.

[0022] Figure 4 This is a cross-sectional schematic diagram of the wax-coated constraint component of the present invention.

[0023] Figure 5 This is a three-dimensional schematic diagram of the four-sided tray of the present invention.

[0024] Figure 6 This is a three-dimensional schematic diagram of the left guide rod of the present invention.

[0025] Figure 7 This is a cross-sectional schematic diagram of the internal parts of the right guide rod of the present invention.

[0026] Figure 8 This is a three-dimensional schematic diagram of the wedge-shaped baffle, reversing block, and reversing inclined rail of the present invention.

[0027] Figure 9 This is a three-dimensional schematic diagram of the slice shifting component of the present invention.

[0028] in,

[0029] 1. Chassis; 101. Chassis door; 102. Heat exchange aisle.

[0030] 2. Slicing linear driver, 201. Slicing blade,

[0031] 3. Feed linear motor, 301, lifting bracket,

[0032] 4. Left support pillar, 401. Displacement clearance groove.

[0033] 5. Right pillar,

[0034] 6. Wax-coated constraint components,

[0035] 601, Four-sided tray; 6011, Rod groove; 6012, Angled through opening.

[0036] 602, left guide rod; 6021, obstruction and clearance groove.

[0037] 603. Right guide rod; 6031. Wedge-shaped baffle; 6032. Reversing block; 6033. Reversing inclined rail; 6034. Pulling core rod.

[0038] 604, obstructing the linear actuator; 6041, obstructing the traction disc.

[0039] 605. Left side abutment plate; 6051. Abutment sliding fork.

[0040] 606. Expansion nails

[0041] 7. Slice shifting component,

[0042] 701. Pitch adjustment motor; 702. Swing frame; 703. Linear displacement actuator.

[0043] 704. Pressure control plate; 705. Piston rod; 706. Piston cylinder; 707. Rigid suction cup; 708. Pressure control linear actuator.

[0044] 8. Display tray.

[0045] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof. Detailed Implementation

[0046] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0047] In the description of this invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0048] like Figures 1-9 As shown, a tumor tissue section sampling device for tumor immunoassay includes a chassis 1, a wax-coating constraint assembly 6, a section shifting assembly 7, a section linear actuator 2, a sectioning blade 201, a feed linear motor 3, and a support bracket 301. The wax-coating constraint assembly 6 and the section shifting assembly 7 are located inside the chassis 1. The section linear actuator 2 is fixedly mounted on the top of the chassis 1. The sectioning blade 201 is fixedly mounted on the moving end of the section linear actuator 2. The feed linear motor 3 is fixedly mounted on the bottom of the chassis 1. The support bracket 301 is fixedly mounted on the moving end of the feed linear motor 3.

[0049] like Figures 1-9 As shown, the chassis 1 is fixedly equipped with a left support column 4 and a right support column 5. The wax-coating constraint assembly 6 includes a four-sided tray 601, a left guide rod 602, and a right guide rod 603. The four-sided tray 601 is fixedly mounted on the support bracket 301. The left guide rod 602 is fixedly connected to the left support column 4, and the right guide rod 603 is fixedly connected to the right support column 5. The four-sided tray 601 is provided with a rod groove 6011. The left guide rod 602 is slidably connected to the rod groove 6011, and the right guide rod 603 is slidably connected to the rod groove 6011. The left guide rod 602 and the right guide rod 603 are fixedly connected. The connecting surface of the left guide rod 602 and the right guide rod 603 coincides with the center surface of the slicing blade 201. The left side and the top of the four-sided tray 601 are empty. The four-sided tray 601 is used to place wax-coated samples.

[0050] like Figures 1-9 As shown, the wax-coated constraint assembly 6 further includes a wedge-shaped baffle 6031, a reversing block 6032, a reversing inclined rail 6033, and a traction core rod 6034. The traction core rod 6034 is slidably connected to the right guide rod 603. The reversing block 6032 is slidably connected to the left end of the traction core rod 6034 in a direction perpendicular to the axis of the right guide rod 603. The reversing inclined rail 6033 is fixedly installed inside the right guide rod 603. The reversing block 6032 is slidably connected to the reversing inclined rail 6033. The wedge-shaped baffle 6031 is fixedly installed at the left end of the reversing block 6032. The right end of the left guide rod 602 is provided with a blocking and clearance groove 6021 adapted to the wedge-shaped baffle 6031. The four-sided support box 601 is provided with an oblique through-hole 6012 communicating with the rod groove 6011.

[0051] like Figures 1-9 As shown, the wax-coated constraint assembly 6 also includes a blocking linear actuator 604 and a blocking traction plate 6041. The blocking linear actuator 604 is fixedly connected to the right support column 5, the blocking traction plate 6041 is fixedly connected to the moving end of the blocking linear actuator 604, and the blocking traction plate 6041 is fixedly connected to the traction core rod 6034.

[0052] like Figures 1-9 As shown, the wax-coating constraint assembly 6 also includes a left-side clamping plate 605, a clamping sliding fork 6051, and an expansion pin 606. The left-side clamping plate 605 is used to clamp the left side of the wax-coated sample. The sliding fork is slidably connected to the left-side clamping plate 605 in the front-back direction and is slidably connected to the four-sided tray 601 in the front-back direction. The left-side clamping plate 605 and the four-sided tray 601 are both provided with nail holes on the right side that are adapted to the expansion pin 606. The expansion pin 606 is used to nail into the left and right ends of the wax-coated sample.

[0053] like Figures 1-9 As shown, the left support column 4 is provided with a displacement clearance groove 401. The slice displacement assembly 7 includes a pitch adjustment motor 701, a swing frame 702, a displacement linear actuator 703, a pressure control plate 704, a piston rod 705, a piston cylinder 706, a rigid suction cup 707, and a pressure control linear actuator 708. The pitch adjustment motor 701 is fixedly connected to the left support column 4. The swing frame 702 is fixedly mounted on the rotating end of the pitch adjustment motor 701. The displacement linear actuator 703 is fixedly mounted on the swing frame 702. The pressure control plate 704 is fixedly mounted on the moving end of the displacement linear actuator 703. The piston rod 705 is fixedly connected to the pressure control plate 704. The piston cylinder 706 is slidably connected to the piston rod 705. The rigid suction cup 707 is fixedly connected to the piston cylinder 706. The pressure control linear actuator 708 is fixedly mounted on the piston cylinder 706. The moving end of the pressure control linear actuator 708 is fixedly connected to the pressure control plate 704.

[0054] like Figures 1-9 As shown, the left side of the chassis 1 is detachably provided with a display tray 8.

[0055] like Figures 1-9 As shown, a heat exchange channel 102 is provided at the rear of the chassis 1, and a door 101 is provided at the front of the chassis 1.

[0056] The tumor tissue is impregnated with paraffin and molded to form a wax-coated sample, ensuring that the size of the wax-coated sample matches the internal dimensions of the four-sided holder 601. It is then placed into the four-sided holder 601 and clamped and positioned by expansion pins 606 and the left-side clamping plate 605.

[0057] The feed linear motor 3 performs periodic feeding, driving the four-sided tray 601 to move to the left via the lifting bracket 301. The slicing linear driver 2 drives the slicing blade 201 to reciprocate at high speed, slicing the wax-coated sample. After all slicing processes are completed, the feed linear motor 3 returns to the zero position.

[0058] The feed linear motor 3, through the lifting bracket 301 and the four-sided support box 601, drives the wax-coated sample back to the zero position. At this time, the wedge-shaped baffle 6031, located at the connection surface of the left guide rod 602 and the right guide rod 603, is directly opposite the slice formed by the first cut by the slicing tool 201. The blocking linear actuator 604 retracts, and through the blocking traction plate 6041, the traction core rod 6034, and the reversing block 6032, it drives the wedge-shaped baffle 6031 to extend along the inclined throughlet 6012 and insert it between the uncut left end of the wax-coated sample and the slice formed by the first cut.

[0059] The operator opens the cabinet door 101, removes the sliding fork, and removes the left-side clamping plate 605 and the uncut left end of the wax-coated sample connected by the expansion pin 606 from the left side of the four-sided tray 601. During this process, the wedge-shaped baffle 6031 prevents the wax-coated sample slice from being removed synchronously due to the adhesion between it and the wax-coated sample on the left. The linear actuator 703 in the slice displacement assembly 7 extends, causing the rigid suction cup 707 to press against the leftmost slice, preventing the linear actuator 604 from returning to the right guide rod 603 with the wedge-shaped baffle 6031. At this point, the preparation work before removing the slice is completed.

[0060] The specific process for slicing removal is as follows: The feed linear motor 3 feeds to the left periodically again according to the feed amount during the previous cutting. During one cycle of feed linear motor 3 to the left, the wax-coated sample in the four-sided tray 601 moves by one slice thickness. At this time, the blocking linear driver 604 retracts, and through the blocking traction plate 6041, the traction core rod 6034, and the reversing block 6032, the wedge-shaped baffle 6031 extends along the inclined through-hole 6012 and is inserted between the leftmost slice and the second slice from the left, to avoid the adhesion between multiple slices affecting the slice thickness.

[0061] With the wedge-shaped stop blocking the second slice from the left, the displacement linear actuator 703 retracts, and the pressure-controlled linear actuator 708 extends by the same distance. Without changing the position of the rigid suction cup 707, the relative position of the piston rod 705 and the piston cylinder 706 is adjusted to create a negative pressure in the inner cavity of the piston cylinder 706 on one side of the rigid suction cup 707, thus sucking up the leftmost slice. Subsequently, the pitch adjustment motor 701 rotates, and through the swing frame 702, the displacement linear actuator 703, the pressure-controlled plate 704, the piston rod 705, the piston cylinder 706, the rigid suction cup 707, and the slice swing through the displacement clearance groove 401. At this time, the pressure-controlled linear actuator 708 retracts and releases the negative pressure effect of the rigid suction cup 707 on the slice, allowing the slice to fall into the slide tray 8.

[0062] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A tumor tissue section sampling device for tumor immunoassay, characterized in that: The system includes a chassis (1), a wax-coating constraint assembly (6), a slicing shift assembly (7), a slicing linear driver (2), a slicing blade (201), a feed linear motor (3), and a support bracket (301). The wax-coating constraint assembly (6) is located inside the chassis (1), the slicing shift assembly (7) is located inside the chassis (1), the slicing linear driver (2) is fixedly mounted on the top of the chassis (1), the slicing blade (201) is fixedly mounted on the moving end of the slicing linear driver (2), the feed linear motor (3) is fixedly mounted on the bottom of the chassis (1), and the support bracket (301) is fixedly mounted on the moving end of the feed linear motor (3).

2. The tumor tissue section sampling device for tumor immunoassay according to claim 1, characterized in that: The chassis (1) is fixedly equipped with a left support column (4) and a right support column (5). The wax-coated constraint assembly (6) includes a four-sided support box (601), a left guide rod (602), and a right guide rod (603). The four-sided support box (601) is fixedly mounted on the support bracket (301). The left guide rod (602) is fixedly connected to the left support column (4), and the right guide rod (603) is fixedly connected to the right support column (5). The four-sided support box (601) is provided with a rod groove (6). 011), the left guide rod (602) is slidably connected to the rod groove (6011), the right guide rod (603) is slidably connected to the rod groove (6011), the left guide rod (602) and the right guide rod (603) are fixedly connected, the connecting surface of the left guide rod (602) and the right guide rod (603) coincides with the center surface of the slicing tool (201), the left side and the top of the four-sided tray (601) are empty, and the four-sided tray (601) is used to place wax-coated samples.

3. The tumor tissue section sampling device for tumor immunoassay according to claim 2, characterized in that: The wax-coated constraint assembly (6) further includes a wedge-shaped baffle (6031), a reversing block (6032), a reversing inclined rail (6033), and a traction core rod (6034). The traction core rod (6034) is slidably connected to the right guide rod (603). The reversing block (6032) is slidably connected to the left end of the traction core rod (6034) in a direction perpendicular to the axis of the right guide rod (603). The reversing inclined rail (6033) is fixed. The reversing block (6032) is slidably connected to the reversing inclined rail (6033) and fixedly installed in the right guide rod (603). The wedge-shaped baffle (6031) is fixedly installed at the left end of the reversing block (6032). The right end of the left guide rod (602) is provided with a blocking and clearance groove (6021) that is adapted to the wedge-shaped baffle (6031). The four-sided support box (601) is provided with an oblique through-hole (6012) that communicates with the rod groove (6011).

4. The tumor tissue section sampling device for tumor immunoassay according to claim 3, characterized in that: The wax-coated constraint assembly (6) further includes a blocking linear actuator (604) and a blocking traction disc (6041). The blocking linear actuator (604) is fixedly connected to the right support column (5), the blocking traction disc (6041) is fixedly connected to the moving end of the blocking linear actuator (604), and the blocking traction disc (6041) is fixedly connected to the traction core rod (6034).

5. A tumor tissue section sampling device for tumor immunoassay according to claim 4, characterized in that: The wax-coating constraint assembly (6) further includes a left-side clamping plate (605), a clamping slide fork (6051), and an expansion pin (606). The left-side clamping plate (605) is used to clamp the left side of the wax-coated sample. The slide fork is slidably connected to the left-side clamping plate (605) in the front-back direction and is slidably connected to the four-sided tray (601) in the front-back direction. The right side of the left-side clamping plate (605) and the four-sided tray (601) are provided with nail holes adapted to the expansion pin (606). The expansion pin (606) is used to nail into the left and right ends of the wax-coated sample.

6. The tumor tissue section sampling device for tumor immunoassay according to claim 5, characterized in that: The left support column (4) is provided with a displacement clearance groove (401). The slice displacement assembly (7) includes a pitch adjustment motor (701), a swing frame (702), a displacement linear actuator (703), a pressure control plate (704), a piston rod (705), a piston cylinder (706), a rigid suction cup (707), and a pressure control linear actuator (708). The pitch adjustment motor (701) is fixedly connected to the left support column (4). The swing frame (702) is fixedly mounted on the rotating end of the pitch adjustment motor (701). The displacement linear actuator... (703) is fixedly mounted on the swing frame (702), the pressure control plate (704) is fixedly mounted on the moving end of the displacement linear actuator (703), the piston rod (705) is fixedly connected to the pressure control plate (704), the piston cylinder (706) is slidably connected to the piston rod (705), the rigid suction cup (707) is fixedly connected to the piston cylinder (706), the pressure control linear actuator (708) is fixedly mounted on the piston cylinder (706), and the moving end of the pressure control linear actuator (708) is fixedly connected to the pressure control plate (704).

7. A tumor tissue section sampling device for tumor immunoassay according to claim 6, characterized in that: The left side of the chassis (1) is detachably equipped with a display tray (8).

8. A tumor tissue section sampling device for tumor immunoassay according to claim 7, characterized in that: A heat exchange channel (102) is provided at the rear of the chassis (1), and a door (101) is provided at the front of the chassis (1).

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