Micropipettor for PCR (Polymerase Chain Reaction) experiment
By incorporating a flexible pipette tip, groove, and pressure relief slot into the micropipette, the problems of liquid backflow and overflow are solved, resulting in higher fixation stability and experimental cleanliness, and improving the safety of the device.
Patent Information
- Application Number
- CN202511195334.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-25
- Publication Date
- 2025-11-18
AI Technical Summary
Existing micropipettes are prone to liquid backflow and overflow during high-speed aspiration, affecting the cleanliness of the experiment. Furthermore, the tiny gap between the pipette tip and the conical interface of the pipette results in poor fixation.
Design a micropipette for PCR experiments, which adopts a structure of elastic tip, groove, protrusion and pressure relief groove. The backflow liquid is reintroduced into the elastic tip through the U-shaped contact seam and the inclined pressure relief groove to prevent overflow and reduce tip deformation.
It effectively prevents liquid spillage, improves the secure fixation of pipette tips and pipettes, and ensures experimental cleanliness and the safety of the equipment.
Smart Images
Figure CN120961248A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of micropipette, more particularly, the present application relates to a micropipette for PCR experiment. BACKGROUND
[0002] PCR, which is short for polymerase chain reaction, is a process of copying the complementary DNA strand from the mother strand DNA under the catalysis of DNA polymerase, and is a DNA in vitro synthesis amplification technology, which can quickly and specifically amplify any target DNA in vitro. In the PCR experiment, a micropipette is used for solution transfer. The micropipette is a precision instrument for measuring 0.1 microliter to 10 milliliter liquid volume, and is an essential tool for sample collection and transfer in biological, chemical and clinical experiments.
[0003] Currently, the micropipette is usually vertically inserted into the elastic suction head at the end of the micropipette, and the suction head can be tightened by slightly rotating left and right with light pressure to perform liquid suction work. However, since the current suction head and the tapered interface of the micropipette rely on friction force for sealing, there is a micron-level gap, especially when large-capacity pipetting, liquid can easily overflow from the gap between the suction head and the end of the micropipette, thereby causing contamination of the end of the micropipette, affecting the cleanliness of the experiment, and when high-speed pipetting (such as 96-well plate operation), the neck of the suction head may be affected by the existence of the small gap, causing instantaneous deformation to cause liquid backflow. SUMMARY
[0004] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present application provide a micropipette for PCR experiment, by setting an elastic suction head, a groove, a protrusion and a pressure relief slot, when the micropipette performs high-speed pipetting and liquid backflow occurs, the liquid flows into the U-shaped contact gap between the suction head connecting cone and the groove, and then flows out along the pressure relief slot on one side of the contact gap, so as to re-discharge the liquid into the inside of the elastic suction head, prevent it from further overflowing from the gap between the suction head connecting cone and the groove, and after the liquid backflow is drained, the pressure of the elastic suction head can be reduced, preventing the contact gap of the suction head connecting cone from being further expanded and squeezed, so as to solve the problems raised in the above background.
[0005] In order to achieve the above object, the present application provides the following technical scheme: A micro pipette for PCR experiment, comprising a pipette, a handle is arranged on the side of the pipette, a connecting part is arranged in communication at the bottom end of the pipette, a joystick is arranged at the top end of the pipette, a suction head connecting cone is arranged in communication at the bottom end of the connecting part, an elastic suction head is detachably sleeved at the end of the suction head connecting cone, a groove is arranged in the inner wall of the elastic suction head, a protrusion is fixedly arranged on one side of the inner wall of the elastic suction head and located in the groove, a plurality of pressure relief slots are arranged in the protrusion, an opening is arranged in communication with the pressure relief slots in the direction close to the inner side of the elastic suction head, the inner side of the suction head connecting cone is embedded into the inside of the groove, a U-shaped contact gap is formed between the suction head connecting cone and the groove, the length of the left side of the contact gap is greater than the length of the right side, the pressure relief slots are in an inclined shape with the left side being higher than the right side, the opening is in an expanded state of a horn mouth, the upper and lower ends of the horn mouth are in an arc shape, the arc length of the bottom end of the opening is greater than the arc length of the top end, and the top end of the opening has a greater arc than the bottom end.
[0006] In a preferred embodiment, the center of the suction head connecting cone and the elastic suction head is located on the same central axis, and the end of the suction head connecting cone and the protrusion is arranged in a circular arc structure.
[0007] In a preferred embodiment, the groove is in interference fit with the suction head connecting cone, and the depth of the groove is between 1cm and 3cm.
[0008] In a preferred embodiment, the protrusion is in a ring structure inside the elastic suction head, and the plurality of pressure relief slots are circumferentially distributed inside the protrusion.
[0009] In a preferred embodiment, after the suction head connecting cone and the groove are embedded into each other, the contact gap is in a completely closed state, and the pressure relief slots are located below and to the right of the contact gap.
[0010] In a preferred embodiment, the inclination of the opening as a whole is consistent with the inclination of the pressure relief slots, and the end of the opening is in communication with the inside of the elastic suction head.
[0011] The technical effects and advantages of the present application are as follows:
[0012] The present application sets the elastic suction head, the groove, the convex and the pressure relief notch, when the liquid returns in the case of high speed liquid suction of the pipette, the liquid flows into the U-shaped contact gap between the suction head connecting cone and the groove, and then is discharged along the pressure relief notch on one side of the contact gap, thereby reducing the liquid pressure, and re-discharging the liquid into the inside of the elastic suction head, preventing the liquid from further overflowing from the gap between the suction head connecting cone and the groove, and after the pressure relief and drainage of the returned liquid, the internal pressure of the elastic suction head is reduced, thereby preventing the liquid from being squeezed into the deep part of the contact gap under the action of pressure, preventing the contact gap between the suction head connecting cone and the groove from being further expanded and squeezed, and the problem of the elastic suction head being squeezed and deformed, further ensuring the fixing firmness between the suction head connecting cone and the elastic suction head, and further improving the use safety of the device. BRIEF DESCRIPTION OF DRAWINGS
[0013] Figure 1 It is a schematic diagram of the overall structure of the present application;
[0014] Figure 2 It is a schematic diagram of the assembly structure of the suction head connecting cone and the elastic suction head of the present application;
[0015] Figure 3 It is a schematic diagram of the side structure of the present application;
[0016] Figure 4 It is a schematic diagram of the installation cross-sectional structure of the suction head connecting cone and the elastic suction head of the present application;
[0017] Figure 5 It is a schematic diagram of the cross-sectional structure of the elastic suction head of the present application;
[0018] Figure 6 It is a schematic diagram of the Figure 5 enlarged structure of A of the present application.
[0019] The figure mark is: 1, pipette; 2, handle; 3, connecting part; 4, operating rod; 5, suction head connecting cone; 6, elastic suction head; 7, groove; 8, convex; 9, pressure relief notch; 10, open port. DETAILED DESCRIPTION
[0020] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.
[0021] As shown in the accompanying drawings Figure 1 and the accompanying drawings Figure 6The utility model provides a kind of PCR experiment micro pipette, including pipette 1, the side of the pipette 1 is provided with handle 2, the bottom end of the pipette 1 is connected with connection part 3, the top end of the pipette 1 is provided with joystick 4, the bottom end of the connection part 3 is connected with suction head connecting cone 5, the tip of the suction head connecting cone 5 is detachably sleeved with elastic suction head 6, recess 7 is set in the inner wall of the elastic suction head 6, the inner wall of the elastic suction head 6 and the recess 7 one side is fixed with protruding 8, the inside of the protruding 8 is provided with multiple pressure relief slots 9, the direction of the pressure relief slots 9 is communicated with opening 10, the inside of the suction head connecting cone 5 is embedded to the inside of recess 7, the suction head connecting cone 5 and recess 7 form a U-shaped contact seam, and the left side length of the contact seam is greater than the right side length, the pressure relief slots 9 are left high right low as a whole, the opening 10 is as a whole in the expansion state of flared mouth, the upper and lower ends of the flared mouth are arc-shaped expansion, and the bottom end trajectory arc length of the opening 10 is greater than the top end arc length, the top end trajectory arc of the opening 10 is greater than the bottom end trajectory arc, by the above setting, when the backflow liquid is guided and discharged by the pressure relief slots 9 and the opening 10, the pressure relief slots 9 and the opening 10 are both inclined to the lower left as a whole, prevent the liquid in the contact seam from being completely discharged, and ensure that the newly drained liquid does not interfere with the backflow liquid in the elastic suction head 6, so that the elastic suction head 6 and the suction head connecting cone 5 are not loosened after being impacted by the liquid, and the practicability of the device is further improved.
[0022] The center of the suction head connecting cone 5 and the elastic suction head 6 is located on the same central axis, the end of the suction head connecting cone 5 and the protruding 8 is provided as a circular arc structure, the recess 7 and the suction head connecting cone 5 are in interference fit, and the depth of the recess 7 is between 1cm and 3cm, the protruding 8 is annular in the inside of the elastic suction head 6, multiple pressure relief slots 9 are circumferentially distributed in the inside of the protruding 8, the inclination of the opening 10 as a whole is consistent with the inclination of the pressure relief slots 9, the end of the opening 10 is connected with the inside of the elastic suction head 6, by the above setting, the interference fit between the suction head connecting cone 5 and the elastic suction head 6 ensures that the two are completely extruded and fitted together, and the contact seam between them is compressed to be sealed, which further increases the fixation of the two and improves the sealing effect of the contact seam, prevents the two from easily loosening and separating, improves the working effect of the device, and the inclination of the opening 10 as a whole is consistent with the inclination of the pressure relief slots 9, which prevents the liquid in the contact seam from being completely discharged, ensures that the newly drained liquid does not interfere with the backflow liquid in the elastic suction head 6, and further improves the working effect of the device.
[0023] With reference to the drawings attached to the specification Figure 6 After the tip connecting cone 5 and the groove 7 are embedded in each other, the contact gap is completely closed as a whole, and the pressure relief gap 9 is located at the lower right of the contact gap.
[0024] The implementation is specific: with the above arrangement, it can be ensured that after the tip connecting cone 5 and the groove 7 are embedded in each other, they are tightly fitted and are not easy to loosen, and at the same time, when liquid backflow occurs during high-speed liquid suction, the liquid backflow will be directly discharged from the pressure relief gap 9 after flowing into the contact gap, thereby re-entering the inside of the elastic tip 6, preventing it from further overflowing from the gap between the tip connecting cone 5 and the groove 7, and reducing the risk of contamination at the end of the pipette 1.
[0025] Working principle of the application:
[0026] First step, first, the operator routinely uses the pipette 1 to perform PCR experiments, and when liquid backflow occurs during high-speed liquid suction of the pipette 1, the liquid flows into the U-shaped contact gap between the tip connecting cone 5 and the groove 7, and then flows out along the pressure relief gap 9 on one side of the contact gap, thereby reducing the liquid pressure while re-discharging the liquid into the inside of the elastic tip 6, preventing it from further overflowing from the gap between the tip connecting cone 5 and the groove 7, and reducing the internal pressure of the elastic tip 6 after the backflow liquid is discharged, thereby preventing the liquid from being squeezed into the deep part of the contact gap under pressure, preventing the contact gap between the tip connecting cone 5 and the groove 7 from being further expanded and squeezed, and preventing the elastic tip 6 from being squeezed and deformed, further ensuring the firmness of the fixation between the tip connecting cone 5 and the elastic tip 6.
[0027] Second step, at the same time, when the backflow liquid is guided and discharged by the pressure relief gap 9 and the open port 10, the pressure relief gap 9 and the open port 10 are both inclined downward as a whole, preventing the liquid in the contact gap from being completely discharged, and ensuring that the re-flowing liquid does not interfere with the backflow liquid in the elastic tip 6, causing the elastic tip 6 and the tip connecting cone 5 to be loosened after being hit by the liquid, further improving the practicality of the device.
[0028] Third step, finally, after completing a series of liquid pipetting operations, the operator can directly pull out and remove the elastic tip 6, and dispose of the consumables, clean the residual liquid inside the pipette 1, and store it vertically for next use.
[0029] Finally should be explained a few points are: first, in the description of the present application, it should be pointed out that, unless otherwise specified and limited, the term "installation", "connected", "connection" should be broad, can be mechanical or electrical connection, but also can be two elements inside the communication, can be directly connected, "up", "down", "left", "right" and so on, only for indicating the relative position relationship, when the absolute position of the described object changes, the relative position relationship may change;
[0030] Second: the present application discloses the embodiment in the drawing, only relates to the structure involved in the present application, other structures can refer to the usual design, in the case of no conflict, the same embodiment and different embodiments of the present application can be combined with each other;
[0031] Finally: the above only for the preferred embodiment of the present application, and not for limiting the present application, any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application, should be included in the protection scope of the present application.
Claims
1. A micropipette for PCR experiments, comprising a pipette, a handle provided on the side of the pipette, a connecting part provided at the bottom of the pipette, an operating lever provided at the top of the pipette, and a pipette tip connecting cone provided at the bottom of the connecting part, characterized in that: The end of the suction head connecting cone is detachably fitted with an elastic suction head. The inner wall of the elastic suction head has a groove, and a protrusion is fixedly provided on one side of the inner wall of the elastic suction head located in the groove. Multiple pressure relief slots are provided inside the protrusion. An open opening is provided in the direction of the pressure relief slot near the inner side of the elastic suction head. The inner side of the suction head connecting cone is fitted into the inside of the groove, and a U-shaped contact seam is formed between the suction head connecting cone and the groove. The left side of the contact seam is longer than the right side. The pressure relief slot is inclined on the left and lower on the right. The open opening is in an expanding state of a trumpet mouth. Both the upper and lower ends of the trumpet mouth are arc-shaped and outwardly expanded. The arc length of the bottom trajectory of the open opening is greater than the arc length of the top trajectory, and the arc length of the top trajectory of the open opening is greater than the arc length of the bottom trajectory.
2. The micropipette for PCR experiments according to claim 1, characterized in that: The centers of the suction head connecting cone and the elastic suction head are located on the same central axis, and the ends of the suction head connecting cone and the protrusion are both set as arc-shaped structures.
3. The micropipette for PCR experiments according to claim 1, characterized in that: The groove and the suction head connecting cone are interference fit, and the depth of the groove is between 1cm and 3cm.
4. The micropipette for PCR experiments according to claim 1, characterized in that: The protrusion is a ring-shaped structure located inside the elastic suction head, and multiple pressure relief slots are circumferentially distributed inside the protrusion.
5. A micropipette for PCR experiments according to claim 1, characterized in that: After the suction head connecting cone and groove are fitted together, the contact seam is completely closed, and the pressure relief groove is located at the lower right of the contact seam.
6. A micropipette for PCR experiments according to claim 1, characterized in that: The overall inclination of the open opening is consistent with the inclination of the pressure relief groove, and the end of the open opening is connected to the interior of the elastic suction head.