Mongolian agropyron cristatum hydroponic nutrient solution formula and preparation method and application thereof

Abstract

The invention discloses a Mongolian agropyron cristatum hydroponic nutrient solution formula as well as a preparation method and application thereof, and belongs to the technical field of agriculture. The Mongolian wheatgrass hydroponic nutrient solution is prepared from sodium silicate nonahydrate, ammonium nitrate, sodium dihydrogen phosphate dihydrate, potassium sulfate, anhydrous calcium chloride, magnesium sulfate heptahydrate, manganese chloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate, copper sulfate pentahydrate, ferrous sulfate heptahydrate and ethylene diamine tetraacetic acid disodium salt. The hydroponic nutrient solution provided by the invention is reasonable in component matching, the Si element necessary for growth of the Mongolian wheatgrass is added, the requirements of the Mongolian wheatgrass on nutrient elements in the growth process, especially in the seedling stage, are met, seedlings using the culture solution disclosed by the invention grow quickly and vigorously, the biomass and nutrient content of overground parts are remarkably increased, and the survival rate of the Mongolian wheatgrass is increased. The high-quality pasture characteristics of the Mongolian wheatgrass are ensured. By means of the water planting technology, the planting range of the Mongolian wheatgrass can be enlarged, growth of the Mongolian wheatgrass is accelerated, and the production quality of the Mongolian wheatgrass is improved.

Description

Technical Field

[0001] This invention relates to the field of agricultural technology, and more specifically to a formulation for a hydroponic nutrient solution for Mongolian ice plant, its preparation method, and its application. Background Technology

[0002] Soilless cultivation is an advanced agricultural technology that uses nutrient solutions, substrates, or semi-substrates to grow crops instead of soil. It rationally utilizes light, temperature, humidity, water, and nutrients to provide the plants with the necessary growth elements, and through precise environmental control techniques, ensures healthy plant growth. Soilless cultivation technology has certain advantages in overcoming the accumulation of harmful substances and pests caused by continuous cropping, reducing yield and quality decline, and also improving land space utilization.

[0003] Hydroponics, also known as nutrient solution culture, is a new soilless cultivation method for plants. Its key feature is the stable supply of ample nutrients to the plant roots, ensuring the plant receives all the necessary micronutrients to meet the growth needs of crops. Hydroponics allows for large-scale plant production in a factory-like environment, regardless of peak or off-peak seasons, and offers advantages such as reduced pesticide use, safety and hygiene, and savings in fertilizer and land resources. Currently, hydroponics is primarily used for flower and vegetable cultivation, and also for the cultivation of medicinal plants.

[0004] Mongolian ice grass ( Agropyron mongolicum Mongolian wheatgrass (Keng) is a perennial herb belonging to the genus *Agrostis* of the tribe Trissae in the Poaceae family. Its morphological characteristics include: long, dense, fibrous roots; sparsely tufted, erect stems; tightly wrapped, smooth, hairless leaf sheaths shorter than the internodes; stiff, involute leaves; and erect, relatively loose spike-like inflorescences. Mongolian wheatgrass is a major vegetation type in desert steppes and sandy areas of typical grasslands. Its tender stems and leaves, good forage quality, and appealing grazing for livestock are significant advantages. In addition to its high forage value, Mongolian wheatgrass also exhibits excellent drought resistance, wind and sand tolerance, cold resistance, and salt and alkali tolerance, making it a valuable forage material for constructing ecological and grazing artificial grasslands, and thus possessing significant potential for widespread application.

[0005] Mongolian crested grass seedlings are weak and less drought-resistant than crested wheatgrass and psammophytic crested wheatgrass, impacting forage yield and quality. Hydroponics, through automated control of cultivation conditions, can provide favorable growth for the plants. Therefore, developing hydroponic technology suitable for Mongolian crested grass growth holds promise for solving the problems of insufficient yield and quality.

[0006] The preparation of nutrient solution is key to obtaining high-yield and high-quality forage. Traditional hydroponic nutrient solution includes the following components: potassium sulfate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, calcium nitrate, boric acid, manganese sulfate, zinc sulfate, copper sulfate, ferrous sulfate heptahydrate, etc. Different types of plants have different nutritional requirements. To meet the specific needs of plant cultivation, the nutrient solution formula needs to be adjusted. For example, patent document CN113149746A discloses a nutrient solution for continuous hydroponic forage cultivation, the main components of which are calcium nitrate tetrahydrate, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, ferrous sulfate heptahydrate, zinc sulfate heptahydrate, manganese sulfate tetrahydrate, copper sulfate pentahydrate, boric acid, sodium chloride, potassium sulfate, and ammonium nitrate. Patent document CN117964426A discloses a hydroponic nutrient solution that can resist root rot of plants, made from the following components: calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, ferric disodium EDTA, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and compound fermentation liquid. This nutrient solution can promote plant growth and prevent root rot when used for seedling cultivation of ice grass, oats, and ryegrass.

[0007] Currently, there are no reports on specific hydroponic nutrient solution formulas for Mongolian icewort. In our previous research, we used nutrient solutions reported in existing literature to hydroponically cultivate Mongolian icewort. The results showed that the growth morphology of Mongolian icewort cultivated under different nutrient solution formulas differed significantly. Therefore, in order to adapt to the hydroponic technology of Mongolian icewort, it is necessary to optimize the hydroponic nutrient solution formula to promote the improvement of Mongolian icewort yield and quality. Summary of the Invention

[0008] The purpose of this invention is to provide a hydroponic nutrient solution and hydroponic method suitable for the growth of Mongolian ice grass, especially for cultivation in the seedling stage, so as to promote the yield and quality improvement of Mongolian ice grass.

[0009] To achieve the above objectives, the present invention adopts the following technical solution: This invention provides a hydroponic nutrient solution for Mongolian icewort, which consists of a base solution and silicates. The base solution per liter comprises: ammonium nitrate 110-120 mg, sodium dihydrogen phosphate dihydrate 45-55 mg, potassium sulfate 85-95 mg, anhydrous calcium chloride 105-115 mg, magnesium sulfate heptahydrate 400-410 mg, manganese chloride tetrahydrate 1.5-2.0 mg, ammonium molybdate tetrahydrate 0.08-0.10 mg, boric acid 1.0-1.2 mg, zinc sulfate heptahydrate 0.03-0.06 mg, copper sulfate pentahydrate 0.02-0.05 mg, ferrous sulfate heptahydrate 30-35 mg, and disodium ethylenediaminetetraacetate 40-50 mg. The silicates are sodium silicate nonahydrate, with a concentration of 150-550 mg / L in the nutrient solution. The solvent for the nutrient solution is water.

[0010] The formula provided by this invention primarily provides nitrogen from ammonium nitrate, phosphorus from sodium dihydrogen phosphate dihydrate, and potassium from potassium sulfate, thus providing essential macronutrients for the growth and development of *Ipomoea quamoclit*. The addition of anhydrous calcium chloride, magnesium sulfate heptahydrate, and potassium sulfate ensures a normal supply of calcium, magnesium, and sulfur during *Ipomoea quamoclit* development. Sodium silicate nonahydrate provides silicon (Si) to *Ipomoea quamoclit*, promoting plant growth, development, and nutrient absorption, and enhancing the plant's resistance to abiotic and biotic stresses. Studies have found that *Ipomoea quamoclit* leaf epidermal cell walls are thick and contain a large amount of silicon in the cell cavities. Adding silicon to the hydroponic nutrient solution can significantly increase the biomass of the upper part of *Ipomoea quamoclit*, and also significantly increase the content of nutrients such as crude protein and crude fat.

[0011] Preferably, the concentration of sodium silicate nonahydrate in the nutrient solution is 300-400 mg / L. Studies have shown that under this concentration condition, the physiological indicators of Mongolian ice grass obtained by hydroponics, such as plant height, root length, aboveground fresh weight, aboveground dry weight, aboveground dry matter content, crude protein content, and crude fat content, are significantly improved.

[0012] More preferably, the concentration of the sodium silicate nonahydrate in the nutrient solution is 350-360 mg / L.

[0013] Preferably, the base solution in each liter of nutrient solution comprises: ammonium nitrate 114-115 mg, sodium dihydrogen phosphate dihydrate 50-51 mg, potassium sulfate 89-90 mg, anhydrous calcium chloride 110-111 mg, magnesium sulfate heptahydrate 405 mg, manganese chloride tetrahydrate 1.8-1.9 mg, ammonium molybdate tetrahydrate 0.09-0.1 mg, boric acid 1.1-1.2 mg, zinc sulfate heptahydrate 0.04-0.05 mg, copper sulfate pentahydrate 0.03-0.04 mg, ferrous sulfate heptahydrate 34-35 mg, and disodium ethylenediaminetetraacetate 46-47 mg.

[0014] As a specific embodiment of the present invention, the components of the base solution per liter of nutrient solution include: ammonium nitrate 114.25 mg, sodium dihydrogen phosphate dihydrate 50.375 mg, potassium sulfate 89.25 mg, anhydrous calcium chloride 110.75 mg, magnesium sulfate heptahydrate 405 mg, manganese chloride tetrahydrate 1.875 mg, ammonium molybdate tetrahydrate 0.0925 mg, boric acid 1.1675 mg, zinc sulfate heptahydrate 0.04375 mg, copper sulfate pentahydrate 0.03875 mg, ferrous sulfate heptahydrate 34.75 mg, and disodium ethylenediaminetetraacetate 46.53 mg.

[0015] In one specific embodiment of the present invention, the concentration of sodium silicate nonahydrate in the nutrient solution is 355.25 mg / L.

[0016] Compared to commonly used hydroponic nutrient solutions such as Hoagland solution, Mongolian ice plant cultured using the above-mentioned nutrient solutions showed a significant increase in plant height, which remained dominant, significant root elongation, and a significant increase in aboveground biomass and nutrient content.

[0017] The present invention also provides a method for preparing the hydroponic nutrient solution for Mongolian ice plant, the method comprising the following steps: (1) Prepare mother liquor A, mother liquor B, mother liquor C, mother liquor D, and mother liquor E by dissolving ammonium nitrate, sodium dihydrogen phosphate dihydrate, potassium sulfate, anhydrous calcium chloride, and magnesium sulfate heptahydrate in water respectively, and set aside; prepare mother liquor F by mixing and dissolving manganese chloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate, and copper sulfate pentahydrate in water, and set aside; prepare mother liquor G by adding disodium ethylenediaminetetraacetate to boiling water until completely dissolved, then adding ferrous sulfate heptahydrate, and heating and stirring until the solution turns brownish-yellow, and set aside; prepare mother liquor H by dissolving sodium silicate nonahydrate in water, and set aside. (2) Measure out mother liquor A, mother liquor B, mother liquor C, mother liquor D, mother liquor E, mother liquor F, mother liquor G and mother liquor H in proportion, mix them, add water to make up the volume, and adjust the pH value to 5.0-5.5 with concentrated hydrochloric acid to obtain the Mongolian ice grass hydroponic nutrient solution.

[0018] Preferably, in step (1), the concentrations of mother liquor A, mother liquor B, mother liquor C, mother liquor D, and mother liquor E are 91.4 g / L, 40.3 g / L, 71.4 g / L, 88.6 g / L, and 324 g / L, respectively. g / L; The concentration of manganese dichloride tetrahydrate in mother liquor F is 1.5 g / L, the concentration of ammonium molybdate tetrahydrate is 0.074 g / L, the concentration of boric acid is 0.934 g / L, the concentration of zinc sulfate heptahydrate is 0.035 g / L, and the concentration of copper sulfate pentahydrate is 0.031 g / L; The concentration of ferrous sulfate heptahydrate in mother liquor G is 6.95 g / L, and the concentration of disodium ethylenediaminetetraacetate is 9.306 g / L; The concentration of sodium silicate nonahydrate in mother liquor H is 142.1 g / L, 284.2 g / L, or 426.3 g / L; In step (2), mother liquor A, mother liquor B, mother liquor C, mother liquor D, mother liquor E, mother liquor F, mother liquor G, and mother liquor H are mixed and dissolved in water in a volume ratio of 1:1:1:1:1:1:1:4:1.

[0019] In this invention, the nutrient solution for Mongolian ice plant hydroponics should be prepared and used immediately to prevent sedimentation from forming after prolonged storage, which would affect the hydroponic effect. Although Mongolian ice plant is an alkali-tolerant plant, excessively high pH values ​​in the nutrient solution can cause sedimentation, which is detrimental to its growth. Lowering the pH value of the nutrient solution can promote the dissolution of elements such as magnesium and calcium ions, thus aiding in plant absorption.

[0020] This invention utilizes the aforementioned nutrient solution to cultivate Mongolian icegrass, significantly increasing its biomass and ensuring its high-quality forage characteristics. This is of great significance for expanding forage cultivation and protecting the grassland ecosystem.

[0021] This invention provides the application of the aforementioned Mongolian icewort hydroponic nutrient solution as a hydroponic nutrient solution for Mongolian icewort seedlings or from seedling to tillering stages. The Mongolian icewort hydroponic nutrient solution provided by this invention meets the nutrient requirements of Mongolian icewort during hydroponic cultivation, especially for seedlings. Mongolian icewort seedlings using this invention's nutrient solution grow rapidly and vigorously.

[0022] The present invention also provides a method for hydroponic cultivation of Mongolian ice grass, the method comprising: transplanting the germinated Mongolian ice grass seedlings into a hydroponic facility containing the above-mentioned Mongolian ice grass hydroponic nutrient solution, wherein the hydroponic conditions are: 22-26℃, 16 h light / 8 h darkness, and the nutrient solution is changed every 7-10 days.

[0023] Preferably, the germination method includes: selecting plump and uniformly sized Mongolian icegrass seeds, peeling off the seed coat of the icegrass using concentrated sulfuric acid, disinfecting the mature embryo with sodium hypochlorite solution and washing it with distilled water, sowing it in 1 / 2 MS solid medium, and germinating it at 22-26℃.

[0024] Seedlings should be transplanted once they have grown to a certain size to ensure they have sufficient growing space. Ideally, the transplanting density of germinating seedlings should be 0.05 seedlings / cm². 2 The transplanting density for 30-day-old Mongolian ice grass seedlings is 0.02 plants / cm². 2 .

[0025] Preferably, the hydroponic facility is made of opaque material, and the nutrient solution is partially shielded from light to prevent algae growth.

[0026] The beneficial effects of this invention are as follows: (1) The hydroponic nutrient solution provided by the present invention has a reasonable composition and meets the nutritional requirements of Mongolian ice grass during its growth process, especially in the seedling stage. Seedlings using the culture solution of the present invention grow rapidly and vigorously, and the aboveground biomass and nutrient content increase significantly, ensuring the high-quality forage characteristics of Mongolian ice grass.

[0027] (2) The present invention adds Si element, which is necessary for the growth of Mongolian ice grass, to the basic culture medium, which is beneficial to the accumulation of Mongolian ice grass biomass and nutrients.

[0028] (3) The hydroponic technology provided by the present invention can expand the planting range of Mongolian ice grass, accelerate the growth of Mongolian ice grass, improve the production quality of Mongolian ice grass, and reduce manual operation and its costs; the hydroponic method can avoid the occurrence of soil-borne diseases and pests, and ensure the safety of the environment for the growth of Mongolian ice grass. Attached Figure Description

[0029] Figure 1 Seedlings of Mongolian ice grass 7 days after germination.

[0030] Figure 2 These are seedlings of Mongolian ice grass grown hydroponically for 10-50 days.

[0031] Figure 3 A statistical chart showing the relative growth of Mongolian ice grass plant height over 10-60 days of hydroponic cultivation.

[0032] Figure 4 A statistical chart showing the relative growth of root length of Mongolian ice plant hydroponically grown for 10-60 days.

[0033] Figure 5 The effects of different hydroponic nutrient solutions on the biomass of Mongolian ice plant are shown. A represents the fresh weight of a single plant; B represents the fresh weight of the aboveground parts; C represents the dry weight of the aboveground parts; and D represents the dry matter content of the aboveground parts.

[0034] Figure 6 The effects of different hydroponic nutrient solution formulations on nutrient accumulation in Mongolian ice plant are shown. A represents NDF content; B represents ADF content; C represents CP content; and D represents EE content. Detailed Implementation

[0035] The present invention will be further described below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Any modifications or substitutions made to the methods, steps, or conditions of the present invention without departing from the spirit and essence of the invention are within the scope of the invention.

[0036] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0037] In the following examples, the seeds of *Isodon japonicus* were obtained from Inner Mongolia Grassland Technology Innovation Center Co., Ltd. 1 / 2 MS medium was purchased from Coolaber, catalog number PM1011. Hogrange nutrient solution was purchased from Phygene, catalog number PH1782.

[0038] Example 1 This embodiment provides the stock solution formula for Mongolian ice plant hydroponic nutrient solution (I), as follows: Mother liquor A: 91.4 g NH4NO3 dissolved in water, diluted to 1 L; Mother liquor B: Dissolve 40.3 g NaH2PO4•2H2O in water and bring the volume up to 1 L; Mother liquor C: 71.4 g K2SO4 dissolved in water, diluted to 1 L; Mother liquor D: 88.6 g anhydrous CaCl2 dissolved in water, diluted to 1 L; Mother liquor E: 324 g MgSO4•7H2O dissolved in water, diluted to 1 L; Mother liquor F: 1.5 g MnCl2•4H2O, 0.074 g (NH4)6Mo7O 24 • Dissolve 4H2O, 0.934 g H3BO3, 0.035 g ZnSO4•7H2O, and 0.031 g CuSO4•5H2O in water and bring the volume to 1 L. Mother liquor G: Dissolve 6.95g FeSO4•7H2O and 9.306g EDTA-Na2 in water and bring the volume to 1 L. Note: When preparing mother liquor G, first add EDTA-Na2 to boiling ddH2O until completely dissolved, then add FeSO4•7H2O and heat while stirring constantly (2-3 hours) until the solution turns brownish-yellow.

[0039] Example 2 This embodiment provides a stock solution formula for Mongolian ice plant hydroponic nutrient solution (II). The difference from embodiment 1 is that stock solution H is added. Stock solution H is a sodium silicate nonahydrate solution. 142.1 g of sodium silicate nonahydrate is dissolved in water and the volume is adjusted to 1 L. The concentration of stock solution H is 142.1 g / L. All other aspects are the same as in embodiment 1.

[0040] Example 3 This embodiment provides a stock solution formula for Mongolian ice plant hydroponic nutrient solution (III). The difference from Example 1 is that stock solution H is added. Stock solution H is a sodium silicate nonahydrate solution. 284.2 g of sodium silicate nonahydrate is dissolved in water and the volume is adjusted to 1 L. The concentration of stock solution H is 284.2 g / L. All other aspects are the same as in Example 1.

[0041] Example 4 This embodiment provides a stock solution formula for Mongolian ice plant hydroponic nutrient solution (IV). The difference from embodiment 1 is that stock solution H is added. Stock solution H is a sodium silicate nonahydrate solution. 426.3 g of sodium silicate nonahydrate is dissolved in water and the volume is adjusted to 1 L. The concentration of stock solution H is 426.3 g / L. All other aspects are the same as in embodiment 1.

[0042] Example 5 In this embodiment, the mother liquors from Examples 1-4 were used to prepare hydroponic nutrient solutions for Mongolian ice plant, as follows: 5 mL each of mother liquor AF, 20 mL of mother liquor G, and 5 mL of mother liquor H were taken, and water was added to bring the volume to 4 L and mixed thoroughly. The pH value was adjusted to 5.0-5.5 with concentrated hydrochloric acid.

[0043] Example 6 This embodiment uses the hydroponic nutrient solution prepared in Example 5 to cultivate Mongolian icewort using hydroponics, with Hogland nutrient solution used as a control group. The Hogland nutrient solution formula is as follows: per liter, it contains 607 mg potassium sulfate, 115 mg ammonium dihydrogen phosphate, 493 mg magnesium sulfate, 20 mg EDTA sodium iron salt, 2.86 mg ferrous sulfate, 4.5 mg borax, 2.13 mg manganese sulfate, 0.05 mg copper sulfate, 0.22 mg zinc sulfate, 0.02 mg ammonium sulfate, and calcium salts.

[0044] 1. The detailed steps for hydroponic cultivation of Mongolian ice plant are as follows: (1) Selection of equipment for Mongolian ice grass cultivation: black hydroponic boxes and opaque hydroponic troughs are used for hydroponics. Black floats are made to prevent the subsequent growth of algae and affect the growth of plants.

[0045] (2) Select plump, uniformly sized Mongolian wheatgrass seeds. Peel the seed coat using concentrated sulfuric acid. Disinfect mature embryos with 10% sodium hypochlorite for 15 min, wash with distilled water, and sow 20-30 seeds per dish in 1 / 2 MS solid medium. Germinate in a 26 ℃ incubator. Figure 1 As shown.

[0046] (3) The germinated seedlings were transplanted into the hydroponic nutrient solutions prepared in Example 5, with a transplanting density of 0.05 seedlings / cm². 2 The cultivation conditions are: 26℃, 16 h light / 8 h dark. The nutrient solution needs to be changed once a week.

[0047] (4) Transplant 30-day-old Mongolian icegrass seedlings to a height of 25cm×24cm×35cm, with a transplanting density of 0.02 plants / cm². 2 In each hydroponic container, add 20 liters of nutrient solution.

[0048] (5) Change the nutrient solution every ten days, and measure the growth of Mongolian ice grass plant height and root length every ten days. The growth rate is calculated using the formula ΔL. （增长量） =L （测量值） -L （初始值） , where L （测量值） L represents the height or root length of a single *Agrostis spp.* plant measured every 10 days. （初始值） This represents the initial plant height and root length of the ice plant when it is hydroponically grown 7 days after germination. This calculation method can eliminate the differences caused by different initial growth states of the ice plant during hydroponics, and can also intuitively show the influence of nutrient solution on the growth of ice plant.

[0049] (6) After 60 days of cultivation, the fresh weight of a single plant, the fresh weight of the aboveground part of a single plant, and the weight of a single plant were statistically analyzed for Mongolian ice grass cultivated in different embodiments. After sampling, the plants were dried at 75℃ for 48 hours and the dry weight was calculated. The dry matter content (DMC) was obtained by dividing the dry weight by the fresh weight and multiplying by 100%.

[0050] (7) Take samples from 60-day-old Mongolian ice grass seedlings 2 cm above the root base, dry them at 75℃ for 48 h, and then measure the nutritional components, including neutral detergent fiber (NDF, %), acid detergent fiber (ADF, %), crude protein (CP, %), and crude fat (EE, %). The measurement method is as follows (Zhang Liying. Feed Analysis and Feed Quality Testing Technology [M]. Beijing: China Agricultural University Press, 2016.).

[0051] Neutral detergent fiber (NDF, %) mainly contains three cell wall components: hemicellulose, cellulose, and lignin. In plants, NDF increases with plant maturity, while NDF digestibility decreases. Increased NDF reduces dry matter intake. Acid detergent fiber (ADF, %) generally shows a negative correlation with digestibility: higher feed ADF corresponds to lower digestibility. Crude protein (CP, %) directly affects animal growth, reproduction, milk production, and other production performance. Crude fat (EE, %) has a significant impact on animal energy supply, physiological metabolism, and product quality.

[0052] 2. Results Analysis Seedlings of Mongolian ice grass 7 days after seed germination Figure 1 As shown. Seedlings that have germinated are transferred to a hydroponic nutrient solution and cultured for 10-50 days. Figure 2 As shown.

[0053] The statistical results of the plant height growth of Mongolian ice plant hydroponically for 10-60 days are as follows: Figure 3 As shown, the statistical results of root length growth are as follows: Figure 4 As shown, n=12, and different lowercase letters indicate significant differences in the measurement results. P <0.05. Analysis of the growth trends of Mongolian icegrass cultured in five groups using nutrient solutions revealed that, compared to the control group, the root length of Mongolian icegrass cultured in Example 2 was significantly increased; the plant height and root length of Mongolian icegrass cultured in Example 3 were significantly increased and maintained this dominance. This indicates that the biomass of Mongolian icegrass cultured in Example 3 was significantly increased, and the plant grew vigorously.

[0054] The aboveground biomass statistics of Mongolian ice plant after 60 days of hydroponics are as follows: Figure 5 As shown, n=6, and different lowercase letters indicate significant differences in the measurement results. P<0.05. Analysis of the upper biomass of Mongolian meadow grass grown in nutrient solution hydroponics in five groups revealed that, compared with the control group, the upper fresh weight of Mongolian meadow grass cultured in Example 3 was significantly increased, with higher dry matter accumulation.

[0055] The statistical results of the above-ground nutrient composition of Mongolian ice plant after 60 days of hydroponics are as follows: Figure 6 As shown, n=6, and different lowercase letters indicate significant differences in the measurement results. P <0.05. Analysis of the nutrient composition of the upper part of Mongolian icegrass grown in hydroponically in five groups of nutrient solutions revealed that, compared with the control group, the NDF content of Mongolian icegrass cultured in Example 3 was significantly reduced by 11.06%, the ADF content was significantly reduced by 7.74%, the CP content was significantly increased by 62.7%, and the EE content was significantly increased by 78.66%.

[0056] As can be seen, compared with other embodiments, the Mongolian ice grass cultivated in Example 3 has a higher accumulation of nutrients, which can increase feed intake, digestibility and energy, and significantly increase forage quality indicators, thus having higher nutritional and feeding value.

[0057] Therefore, the present invention provides a nutrient solution formula for hydroponic cultivation of Mongolian ice plant and its preparation method, with the optimal addition concentration of each component as shown in Example 3.

[0058] The above description is merely a specific embodiment of the present invention, intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and should not be construed as limiting the scope of protection of the present invention. All equivalent modifications or substitutions made based on the essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A hydroponic nutrient solution for Mongolian ice plant, characterized in that, The nutrient solution consists of a base solution and silicates. The base solution per liter comprises: ammonium nitrate 110-120 mg, sodium dihydrogen phosphate dihydrate 45-55 mg, potassium sulfate 85-95 mg, anhydrous calcium chloride 105-115 mg, magnesium sulfate heptahydrate 400-410 mg, manganese chloride tetrahydrate 1.5-2.0 mg, ammonium molybdate tetrahydrate 0.08-0.10 mg, boric acid 1.0-1.2 mg, zinc sulfate heptahydrate 0.03-0.06 mg, copper sulfate pentahydrate 0.02-0.05 mg, ferrous sulfate heptahydrate 30-35 mg, and disodium ethylenediaminetetraacetate 40-50 mg. The silicates are sodium silicate nonahydrate, with a concentration of 150-550 mg / L in the nutrient solution.

2. The Mongolian ice plant hydroponic nutrient solution as described in claim 1, characterized in that, The concentration of sodium silicate nonahydrate in the nutrient solution is 300-400 mg / L.

3. The Mongolian ice plant hydroponic nutrient solution as described in claim 1, characterized in that, The concentration of sodium silicate nonahydrate in the nutrient solution is 350-360 mg / L.

4. The hydroponic nutrient solution for Mongolian icewort as described in any one of claims 1-3, characterized in that, The base solution in each liter of nutrient solution consists of: ammonium nitrate 114-115 mg, sodium dihydrogen phosphate dihydrate 50-51 mg, potassium sulfate 89-90 mg, anhydrous calcium chloride 110-111 mg, magnesium sulfate heptahydrate 405 mg, manganese chloride tetrahydrate 1.8-1.9 mg, ammonium molybdate tetrahydrate 0.09-0.1 mg, boric acid 1.1-1.2 mg, zinc sulfate heptahydrate 0.04-0.05 mg, copper sulfate pentahydrate 0.03-0.04 mg, ferrous sulfate heptahydrate 34-35 mg, and disodium ethylenediaminetetraacetate 46-47 mg.

5. The method for preparing the hydroponic nutrient solution for Mongolian icewort as described in any one of claims 1-4, characterized in that, Includes the following steps: (1) Prepare mother liquor A, mother liquor B, mother liquor C, mother liquor D, and mother liquor E by dissolving ammonium nitrate, sodium dihydrogen phosphate dihydrate, potassium sulfate, anhydrous calcium chloride, and magnesium sulfate heptahydrate in water respectively, and set aside; prepare mother liquor F by mixing and dissolving manganese chloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate, and copper sulfate pentahydrate in water, and set aside; prepare mother liquor G by adding disodium ethylenediaminetetraacetate to boiling water until completely dissolved, then adding ferrous sulfate heptahydrate, and heating and stirring until the solution turns brownish-yellow, and set aside; prepare mother liquor H by dissolving sodium silicate nonahydrate in water, and set aside. (2) Measure out mother liquor A, mother liquor B, mother liquor C, mother liquor D, mother liquor E, mother liquor F, mother liquor G and mother liquor H in proportion, mix them, add water to make up the volume, and adjust the pH value to 5.0-5.5 with concentrated hydrochloric acid to obtain the Mongolian ice grass hydroponic nutrient solution.

6. The preparation method according to claim 5, characterized in that, In step (1), the concentrations of mother liquor A, mother liquor B, mother liquor C, mother liquor D, and mother liquor E are 91.4 g / L, 40.3 g / L, 71.4 g / L, 88.6 g / L, and 324 g / L, respectively. g / L; The concentration of manganese dichloride tetrahydrate in mother liquor F is 1.5 g / L, the concentration of ammonium molybdate tetrahydrate is 0.074 g / L, the concentration of boric acid is 0.934 g / L, the concentration of zinc sulfate heptahydrate is 0.035 g / L, and the concentration of copper sulfate pentahydrate is 0.031 g / L; The concentration of ferrous sulfate heptahydrate in mother liquor G is 6.95 g / L, and the concentration of disodium ethylenediaminetetraacetate is 9.306 g / L; The concentration of sodium silicate nonahydrate in mother liquor H is 142.1 g / L, 284.2 g / L, or 426.3 g / L; In step (2), mother liquor A, mother liquor B, mother liquor C, mother liquor D, mother liquor E, mother liquor F, mother liquor G, and mother liquor H are mixed and dissolved in water in a volume ratio of 1:1:1:1:1:1:1:4:

1.

7. The application of the Mongolian ice plant hydroponic nutrient solution as described in any one of claims 1-4 as a hydroponic nutrient solution for the seedling stage or the seedling to tillering stage of Mongolian ice plant.

8. A method for hydroponic cultivation of Mongolian ice plant, characterized in that, The method includes: transplanting the germinated Mongolian ice grass seedlings into a hydroponic facility containing the Mongolian ice grass hydroponic nutrient solution as described in any one of claims 1-4, wherein the hydroponic conditions are: 22-26℃, 16h light / 8h darkness, and the nutrient solution is changed every 7-10 days.

9. The method for hydroponic cultivation of Mongolian ice plant as described in claim 8, characterized in that, The germination method includes: selecting plump and uniformly sized Mongolian icegrass seeds, peeling off the seed coat with concentrated sulfuric acid, disinfecting the mature embryos with sodium hypochlorite solution, washing them with distilled water, sowing them in 1 / 2 MS solid medium, and germinating them at 22-26℃.

10. The method for hydroponic cultivation of Mongolian ice plant as described in claim 8, characterized in that, The transplanting density of germinating seedlings is 0.05 plants / cm². 2 The transplanting density for 30-day-old Mongolian ice grass seedlings is 0.02 plants / cm². 2 .

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