Preparation method of eyedrops for preventing eye injury

Abstract

The invention discloses a preparation method of eyedrops for preventing eye injury, and belongs to the technical field of eyedrops, the eyedrops are prepared by taking rhizoma polygonati, herba houttuyniae, sodium chloride, ethylparaben, distilled water and hypertonic liquid as raw materials, and the hypertonic liquid prepared by mixing glucose injection and vitamin C injection in the eyedrops has a dehydration effect in the application process, so that the eye injury can be prevented. The corneal edema can be quickly eliminated, the dehydration of corneal tissues is promoted, and the normal thickness is recovered, so that the vision is quickly recovered, the damage of corneal endothelium is furthest reduced, the corneal edema in the treatment process is effectively reduced, and the probability of cataract is further reduced; according to the traditional Chinese medicine eye drop, the components of rhizoma polygonati and herba houttuyniae are added, so that the eye drop has antibacterial and antiviral effects, rhizoma polygonati polysaccharide contained in rhizoma polygonati has the effects of increasing tear secretion and promoting ocular surface healing, dry eyes, foreign body sensation and burning sensation are improved, and the eye drop has no obvious irritation to cornea and iris and is suitable for different people.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of eye drops, and particularly relates to a preparation method of eye drops for preventing eye injury. BACKGROUND

[0002] Photodynamic therapy is a method of treating eye diseases by using photosensitizers and specific wavelength light. During the treatment, photosensitizers are first injected into the patient's body, and the photosensitizers selectively accumulate in the diseased tissues, especially in the newly formed blood vessels. Subsequently, the diseased area is irradiated with a specific wavelength laser to activate the photosensitizers to produce singlet oxygen and free radicals, which can destroy the structure of diseased cells and cause cell death, thereby achieving the treatment purpose.

[0003] Although photodynamic therapy is widely used in the field of ophthalmology, it may also cause some potential harm. During the photodynamic therapy, photosensitizers may stimulate the eye and cause redness and swelling. Moreover, patients who receive long-term photodynamic therapy, especially special groups such as the elderly, are prone to increase the risk of cataracts. Although the conventional eye drops can alleviate the symptoms, they are difficult to prevent the above-mentioned problems, have low sustainable efficacy, and some eye drops are too irritating to cause discomfort to some people. SUMMARY

[0004] In order to overcome the above-mentioned defects, the application provides a preparation method of eye drops for preventing eye injury, which solves the problems that photosensitizers may stimulate the eye and cause redness and swelling during photodynamic therapy, and patients who receive long-term photodynamic therapy, especially special groups such as the elderly, are prone to increase the risk of cataracts. Although the conventional eye drops can alleviate the symptoms, they are difficult to prevent the above-mentioned problems, have low sustainable efficacy, and some eye drops are too irritating to cause discomfort to some people.

[0005] To achieve the above-mentioned purpose, the application provides the following technical scheme: a preparation method of eye drops for preventing eye injury, comprising the following steps: S1, preparing the following components and their weight ratios of raw materials: Huangji 2-3 parts, Houttuynia cordata 10-15 parts, sodium chloride 3-5 parts, hydroxyphenyl ethyl ester 0.1-0.3 parts, distilled water 20-30 parts, and high osmotic solution 30-35 parts; S2, after pretreating Huangji, adding distilled water and activated carbon for injection, boiling for 30-35 min, and naturally cooling, the mixture is stored at 4-5 DEG C for 24 h to obtain Huangji eye drops; S3, Houttuynia cordata is added with 10 times the amount of water, distilled and extracted, and 6 times the amount of aromatic water is collected for standby; S4, the standing of Huangjing medicine water is filtered, and then aromatic water, sodium chloride and hydroxyphenyl ethyl ester are added and stirred uniformly, then distilled water is added and pH adjuster is used for adjustment, and after mixing, micro-porous membrane is used for fine filtration to obtain clear liquid; S5, the clear liquid is fully mixed with high osmotic solution, sterilized at 105 DEG C for 30-40 min, and then aseptically packed in containers to complete the preparation of eye drops.

[0006] As a further scheme of the application, the high osmotic solution is mixed and configured from glucose injection and vitamin C injection, the concentration of the glucose injection is 50%, and the mass ratio of the glucose injection to the vitamin C injection is 20-25:3-5.

[0007] As a further scheme of the application, in S2, the step of pretreating Huangjing is: Huangjing is soaked in water for 1 h, reflux extracted twice, 10 times water is used for the first time for 2.5 h, 8 times water is used for the second time for 2 h, and the filtrates of the two times are filtered and combined, and concentrated at 70-80 DEG C under reduced pressure to a relative density of 1.08-1.10; After the concentrated solution is placed at room temperature, stirring is carried out, ethanol is added under stirring to reach an alcohol content of %, then it is statically placed for 24-30, the precipitate is taken after filtration, and then it is heated and dissolved to complete the pretreatment of Huangjing.

[0008] As a further scheme of the application, in S2, 0.2% (g / mL) of injection activated carbon is added, and the injection activated carbon is dried in an oven at 150 DEG C for 4-5 h for activation before use.

[0009] As a further scheme of the application, in S4, the pH adjuster is 10% sodium hydroxide solution, the pH is adjusted to 7.0, and the micro-porous membrane is 0.45 mu m.

[0010] Compared with the prior art, the application has the beneficial effects that the eye drops prepared from Huangjing, Houttuynia cordata, sodium chloride, hydroxyphenyl ethyl ester, distilled water and high osmotic solution are dropped on the eyes of the patient before photodynamic therapy, the high osmotic solution mixed and configured from glucose injection and vitamin C injection has a dehydrating effect in the application process, can quickly eliminate corneal edema, promote corneal tissue dehydration and restore normal thickness, thereby quickly restore vision, maximize the reduction of damage to the corneal endothelium, effectively reduce the occurrence of corneal edema during treatment, and further reduce the probability of cataract, the addition of Huangjing and Houttuynia cordata components makes the eye drops have antibacterial and antiviral effects, the Huangjing polysaccharide contained in Huangjing has the effects of increasing tear secretion and promoting the healing of the ocular surface, improving eye dryness, foreign body sensation and burning sensation, and the eye drops have no obvious irritation to the cornea and iris, and are suitable for different populations. Detailed Implementation

[0011] The technical solution of this application will be further described in detail below with reference to specific embodiments.

[0012] This invention provides a technical solution: a method for preparing eye drops to prevent eye damage, comprising the following steps: S1. Prepare the following components and their weight ratios: 2-3 parts Polygonatum sibiricum, 10-15 parts Houttuynia cordata, 3-5 parts sodium chloride, 0.1-0.3 parts ethylparaben, 20-30 parts distilled water, and 30-35 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum decoction. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum herb solution by suction, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

[0013] The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, with the glucose injection concentration being 50% and the mass ratio of glucose injection to vitamin C injection being 20-25:3-5.

[0014] In S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

[0015] In S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is dried in an oven at 150℃ for 4-5 hours before use to activate it.

[0016] In S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm.

[0017] Example 1: A method for preparing eye drops to prevent eye damage includes the following steps: S1. Prepare the following components and their weight ratios: 3 parts Polygonatum sibiricum, 12 parts Houttuynia cordata, 3 parts sodium chloride, 0.1 parts ethylparaben, 25 parts distilled water, and 32 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum decoction. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum herb solution by suction, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

[0018] The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, with the glucose injection concentration being 50% and the mass ratio of glucose injection to vitamin C injection being 20-25:3-5.

[0019] In S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

[0020] In S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is dried in an oven at 150℃ for 4-5 hours before use to activate it.

[0021] In S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm.

[0022] Example 2: A method for preparing eye drops to prevent eye damage includes the following steps: S1. Prepare the following components and their weight ratios: 3 parts Polygonatum sibiricum, 12 parts Houttuynia cordata, 3 parts sodium chloride, 0.1 parts ethylparaben, 25 parts distilled water, and 35 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum decoction. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum herb solution by suction, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

[0023] The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, with the glucose injection concentration being 50% and the mass ratio of glucose injection to vitamin C injection being 20-25:3-5.

[0024] In S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

[0025] In S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is dried in an oven at 150℃ for 4-5 hours before use to activate it.

[0026] In S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm.

[0027] Example 3: A method for preparing eye drops to prevent eye damage includes the following steps: S1. Prepare the following components and their weight ratios: 3 parts Polygonatum sibiricum, 10 parts Houttuynia cordata, 3 parts sodium chloride, 0.1 parts ethylparaben, 30 parts distilled water, and 31 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum decoction. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum herb solution by suction, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

[0028] The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, with the glucose injection concentration being 50% and the mass ratio of glucose injection to vitamin C injection being 20-25:3-5.

[0029] In S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

[0030] In S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is dried in an oven at 150℃ for 4-5 hours before use to activate it.

[0031] In S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm.

[0032] Example 4: A method for preparing eye drops to prevent eye damage includes the following steps: S1. Prepare the following components and their weight ratios: 2 parts Polygonatum sibiricum, 10 parts Houttuynia cordata, 4 parts sodium chloride, 0.15 parts ethylparaben, 25 parts distilled water, and 33 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum decoction. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum herb solution by suction, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

[0033] The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, with the glucose injection concentration being 50% and the mass ratio of glucose injection to vitamin C injection being 20-25:3-5.

[0034] In S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

[0035] In S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is dried in an oven at 150℃ for 4-5 hours before use to activate it.

[0036] In S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm.

[0037] Example 5: A method for preparing eye drops to prevent eye damage includes the following steps: S1. Prepare the following components and their weight ratios: 3 parts Polygonatum sibiricum, 15 parts Houttuynia cordata, 4 parts sodium chloride, 0.2 parts ethylparaben, 25 parts distilled water, and 33 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum decoction. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum herb solution by suction, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

[0038] The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, with the glucose injection concentration being 50% and the mass ratio of glucose injection to vitamin C injection being 20-25:3-5.

[0039] In S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

[0040] In S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is dried in an oven at 150℃ for 4-5 hours before use to activate it.

[0041] In S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm.

[0042] Example 6: A method for preparing eye drops to prevent eye damage includes the following steps: S1. Prepare the following components and their weight ratios: 2 parts Polygonatum sibiricum, 10 parts Houttuynia cordata, 3 parts sodium chloride, 0.1 parts ethylparaben, 30 parts distilled water, and 30 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum decoction. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum herb solution by suction, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

[0043] The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, with the glucose injection concentration being 50% and the mass ratio of glucose injection to vitamin C injection being 20-25:3-5.

[0044] In S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

[0045] In S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is dried in an oven at 150℃ for 4-5 hours before use to activate it.

[0046] In S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm.

[0047] Several patients participating in photodynamic therapy were selected. Before treatment, the eye drops prepared in Examples 1-6 were instilled into the patients' eyes, and the corneal edema and ocular irritation reactions were observed. The results are shown in the table below:

[0048] The comparison in the table above shows that: The difference between Example 1 and Example 2 is that the amount of hypertonic solution is different but the difference is small, while other raw materials are the same, in order to prepare eye drops to prevent eye damage, so that the patient's eyes are not significantly irritated and the cornea is transparent and without edema. The difference between Example 1 and Example 3 is that the amount of houttuynia cordata and hypertonic solution in Example 3 is lower than that in Example 1, so that the patient's eyes are not significantly irritated, the cornea is transparent without edema, the cornea has localized thin hazy edema, and the corneal endothelial surface is smooth. In Example 6, the components of Polygonatum sibiricum, Houttuynia cordata, and hypertonic solution were all lower than those in Examples 1-2. Although there was no obvious irritation to the patient's eyes, there was slight edema, which had little impact on the patient. Therefore, the eye drops prepared using the formula in Examples 1-2 had the best preventive effect.

[0049] From the above, we know that: This eye drop is a hypertonic solution prepared by mixing glucose injection and vitamin C injection. During application, it has a dehydrating effect, which can quickly eliminate corneal edema, promote corneal tissue dehydration and restore normal thickness, thereby rapidly restoring vision, minimizing damage to the corneal endothelium, effectively reducing the occurrence of corneal edema during treatment, and thus reducing the chance of developing cataracts. The addition of Polygonatum and Houttuynia cordata ingredients gives the eye drop antibacterial and antiviral effects. Polygonatum polysaccharides contained in Polygonatum can increase tear secretion, promote ocular surface healing, and improve dry eyes, foreign body sensation, and burning sensation. Moreover, the eye drop has no obvious irritation to the cornea and iris and is suitable for different groups of people.

[0050] In the description of this invention, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc., indicating orientation or positional relationships are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0051] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0052] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a communication connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0053] In this invention, unless otherwise expressly specified and limited, the first feature "on" or "below" the second feature may be in direct contact with the first and second features, or indirect contact through an intermediate medium. In the description of this specification, references to terms such as "an embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

Claims

1. A method for preparing eye drops to prevent eye damage, characterized in that, Includes the following steps: S1. Prepare the following components and their weight ratios: 2-3 parts Polygonatum sibiricum, 10-15 parts Houttuynia cordata, 3-5 parts sodium chloride, 0.1-0.3 parts ethylparaben, 20-30 parts distilled water, and 30-35 parts hypertonic solution; S2. After pretreatment of Polygonatum, add distilled water and activated carbon for injection, boil for 30-35 minutes, then cool naturally and refrigerate at 4-5℃ for 24 hours to obtain Polygonatum medicinal solution. S3. Add 10 times the amount of water to the houttuynia cordata, distill to extract, and collect 6 times the amount of aromatic water for later use. S4. Filter the settled Polygonatum sibiricum solution, then add aromatic water, sodium chloride and ethylparaben and stir evenly. Next, add distilled water and adjust the pH with a pH adjuster. After mixing, filter the solution through a microporous membrane to obtain a clear liquid. S5. Thoroughly mix the clear liquid and the hypertonic solution, sterilize at 105℃ for 30-40 minutes, and aseptically dispense into containers to complete the preparation of the eye drops.

2. The method for preparing eye drops for preventing eye injury according to claim 1, characterized in that: The hypertonic solution is prepared by mixing glucose injection and vitamin C injection, wherein the concentration of glucose injection is 50% and the mass ratio of glucose injection to vitamin C injection is 20-25:3-5.

3. The method for preparing eye drops for preventing eye injury according to claim 1, characterized in that, In step S2, the pretreatment steps for Polygonatum are as follows: Soak Polygonatum in water for 1 hour, then reflux and extract twice. The first extraction is with 10 times the amount of water for 2.5 hours, and the second extraction is with 8 times the amount of water for 2 hours. Filter and combine the two filtrates, then concentrate under reduced pressure at 70-80℃ to a relative density of 1.08-1.

10. After the concentrate is cooled to room temperature, it is stirred. Ethanol is added while stirring to bring the alcohol content to a certain percentage. Then, it is allowed to stand for 24-30 minutes. After filtration, the precipitate is collected and then heated to dissolve, thus completing the pretreatment of Polygonatum.

4. The method for preparing eye drops for preventing eye injury according to claim 1, characterized in that: In step S2, 0.2% (g / mL) of injectable activated carbon is added, and the injectable activated carbon is activated by drying in an oven at 150℃ for 4-5 hours before use.

5. The method for preparing eye drops for preventing eye injury according to claim 1, characterized in that: In step S4, the pH adjuster is a 10% sodium hydroxide solution, adjusted to pH 7.0, and the microporous membrane is 0.45 μm in diameter.

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