Bacteriostatic and hair-consolidating natural plant extract composition containing metabiotics and preparation method of bacteriostatic and hair-consolidating natural plant extract composition

Abstract

The invention discloses a bacteriostasis and hair consolidation natural plant extract composition containing metabiotics and a preparation method of the bacteriostasis and hair consolidation natural plant extract composition. The composition comprises the following raw materials: an ajowan fruit extract, a lemongrass whole plant extract, a cinnamomum tamala leaf extract, a melaleuca alternifolia branch and leaf extract, a thuja koreana branch and leaf extract, a thyme flower extract, a cacumen biotae extract, a streptococcus thermophilus fermentation product lysate and a yeast / zinc fermentation product. Through reasonable proportioning and synergistic effect of different raw materials, the traditional Chinese medicine composition has a good inhibition effect on malassezia furfur and staphylococcus epidermidis of main microorganism groups causing alopecia seborrhoeica and other scalp diseases. In addition, beneficial components in the plant extract have the effects of promoting hair regeneration, improving scalp blood circulation and the like, and the metagen component can improve scalp microecology and inhibit pathogenic bacterium proliferation, so that the composition has good hair fixing and hair loss preventing effects.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of washing and caring products, and particularly relates to a natural plant extract composition containing postbiotics for inhibiting bacteria and fixing hair and a preparation method and application thereof. BACKGROUND

[0002] Skin is the largest organ of the human body, and is distributed with hundreds of millions of bacteria, fungi, viruses and other types of microorganisms. These microorganisms and their genetic information and functions, through interaction and co-evolution with the human host and the external environment, form a stable skin microecosystem. Skin microecological imbalance may cause various skin diseases such as atopic dermatitis, acne, psoriasis and the like.

[0003] The scalp is an important part of the human skin. Due to the presence of a large number of hair, a large number of hair follicles, sweat glands, sebaceous glands and relatively high humidity and oiliness, the scalp has a unique microecological environment, thus creating a unique microbial community structure of the human scalp. Under normal physiological conditions, the bacterial flora on the surface of a healthy scalp is mainly Propionibacterium acnes and Staphylococcus, and the fungi are mainly Malassezia. These microorganisms maintain the scalp microenvironment and barrier function through mutual cooperation and mutual restraint, and escort the healthy scalp. When the scalp environment is changed by certain internal and external factors, the abnormal proliferation of certain microorganisms such as Malassezia and Staphylococcus epidermidis will occur, thus causing the imbalance of the scalp microecology and inducing or aggravating various scalp diseases such as hair loss, dandruff, seborrheic dermatitis, scalp psoriasis and folliculitis. Therefore, by controlling the abnormal proliferation of Malassezia and Staphylococcus epidermidis on the scalp, the above scalp problems can be alleviated.

[0004] At present, the most common type of hair loss in the world is androgenetic alopecia (also known as seborrheic alopecia), which is highly prevalent in 80% of adult males and 50% of adult females. The main clinical manifestations are excessive secretion of scalp oil, oily hair, increased dandruff and itching. The pathogenesis of androgenetic alopecia is closely related to genetic susceptibility, abnormal activity of 5α-reductase, enhanced sensitivity of androgen receptors, imbalance of cytokine regulation and excessive proliferation of some microorganisms (such as Malassezia and Staphylococcus epidermidis). At present, the treatment of this disease mainly adopts oral administration of finasteride and topical application of minoxidil. However, these two treatment methods still have certain controversies and may be accompanied by adverse reactions. For example, finasteride can cause sexual dysfunction; and after discontinuation of minoxidil, its effect of promoting hair growth will gradually weaken, and it can also cause local side effects such as dry scalp, itching, irritation or allergy. Therefore, seeking a scalp care product that is safer and friendlier, has the effects of improving scalp health and inhibiting bacteria and fixing hair, has become an urgent need of more and more consumers.

[0005] China is a big country of traditional Chinese herbal medicine, and has a long history of using various natural plants. Natural plant extracts are rich in polyphenols, flavonoids, terpenes, aromatics, saponins and other active ingredients, and their multi-target mechanism has unique advantages in regulating sebaceous gland activity, inhibiting Malassezia proliferation and relieving inflammatory response. In addition, natural plant extracts are natural in origin and have the characteristics of mildness and non-irritation, and have gradually become a research hotspot in the field of cosmetics and washing and care products.

[0006] Postbiotics refers to non-living microorganisms and / or preparations of effective components thereof that are beneficial to the health of the host, including metabolites produced during fermentation, active small molecules released by molecular fermentation substrates, dead cells and cell components after lysis, etc. Because there are no living bacteria, postbiotics has the advantages of high safety, good stability, strong adaptability, and can directly play a role without microbial colonization. As a new microecological skin care ingredient, postbiotics has been proven to have a positive effect on improving skin pigmentation, regulating tyrosinase expression and collagen synthesis, inhibiting pathogenic bacteria proliferation, and improving skin microecology, and has a good application prospect in the field of cosmetics and washing and care products. SUMMARY

[0007] In view of the above defects or improvement needs of the prior art, the present application provides a postbiotic-containing antibacterial and hair-fixing natural plant extract composition and a preparation method thereof.

[0008] The technical scheme adopted by the present application is as follows:

[0009] A postbiotic-containing antibacterial and hair-fixing natural plant extract composition, which comprises natural plant extract and postbiotics; the natural plant extract is one or more of Trachyspermum ammi extract, Cymbopogon citratus extract, Cinnamomun zeylanicum extract, Melaleuca alternifolia extract, Thuja koraiensis extract, Thymus vulgaris extract and Platycladus orientalis extract; the postbiotics is one or more of Streptococcus thermophilus fermentation product lysate and yeast / zinc fermentation product.

[0010] Preferably, the A. indica fruit extract is an A. indica fruit extract, the Cymbopogon citratus extract is a Cymbopogon citratus whole plant extract, the Cinnamomum zeylanicum extract is a Cinnamomum zeylanicum leaf extract, the Melaleuca alternifolia extract is a Melaleuca alternifolia branch and leaf extract, the Thuja koraiensis extract is a Thuja koraiensis branch and leaf extract, the Thymus vulgaris extract is a Thymus vulgaris flower extract, and the Platycladus orientalis extract is a Platycladus orientalis leaf extract.

[0011] Preferably, the natural plant extract includes the following raw materials in parts by weight: 20-40 parts of A. indica fruit extract, 15-40 parts of Cymbopogon citratus whole plant extract, 10-30 parts of Cinnamomum zeylanicum leaf extract, 10-30 parts of Melaleuca alternifolia branch and leaf extract, 15-30 parts of Thuja koraiensis branch and leaf extract, 5-20 parts of Thymus vulgaris flower extract, and 5-20 parts of Platycladus orientalis leaf extract.

[0012] Preferably, the probiotic includes the following raw materials in parts by weight: 0.1-6 parts of Streptococcus thermophilus fermentation product lysate and 0.1-5 parts of Saccharomyces / Zinc fermentation product.

[0013] Preferably, the probiotic-containing bacteriostatic and hair-fixing natural plant extract composition includes the following raw materials in parts by weight: 20-40 parts of A. indica fruit extract, 15-40 parts of Cymbopogon citratus whole plant extract, 10-30 parts of Cinnamomum zeylanicum leaf extract, 10-30 parts of Melaleuca alternifolia branch and leaf extract, 15-30 parts of Thuja koraiensis branch and leaf extract, 5-20 parts of Thymus vulgaris flower extract, 5-20 parts of Platycladus orientalis leaf extract, 0.1-6 parts of Streptococcus thermophilus fermentation product lysate, and 0.1-5 parts of Saccharomyces / Zinc fermentation product.

[0014] More preferably, the probiotic-containing bacteriostatic and hair-fixing natural plant extract composition includes the following raw materials in parts by weight: 32-38 parts of A. indica fruit extract, 25-38 parts of Cymbopogon citratus whole plant extract, 20-25 parts of Cinnamomum zeylanicum leaf extract, 20-25 parts of Melaleuca alternifolia branch and leaf extract, 25-30 parts of Thuja koraiensis branch and leaf extract, 15-20 parts of Thymus vulgaris flower extract, 15-20 parts of Platycladus orientalis leaf extract, 0.1-6 parts of Streptococcus thermophilus fermentation product lysate, and 0.1-5 parts of Saccharomyces / Zinc fermentation product.

[0015] More preferably, the probiotic-containing bacteriostatic and hair-fixing natural plant extract composition includes the following raw materials in parts by weight: 36 parts of A. indica fruit extract, 35 parts of Cymbopogon citratus whole plant extract, 23 parts of Cinnamomum zeylanicum leaf extract, 22 parts of Melaleuca alternifolia branch and leaf extract, 28 parts of Thuja koraiensis branch and leaf extract, 16 parts of Thymus vulgaris flower extract, 18 parts of Platycladus orientalis leaf extract, 3 parts of Streptococcus thermophilus fermentation product lysate, and 2.5 parts of Saccharomyces / Zinc fermentation product.

[0016] Ayuwi is a plant of the Umbelliferae genus, and its fruit extract contains various active ingredients, and has good antibacterial, antiseptic, anti-infection and antioxidant effects.

[0017] Citronella is a fragrant plant of the Poaceae genus, and its extract is rich in volatile compounds such as aldehydes, alcohols, esters, terpenes and ketones, and has strong antibacterial and anti-inflammatory effects.

[0018] Ceylon cinnamon is a traditional edible and medicinal plant of the Lauraceae genus, and its extract is rich in terpenes, flavonoids and aromatic compounds, and has antibacterial, antioxidant and anti-aging effects.

[0019] Mutual leaf white jasmine is a plant of the Myrtaceae genus, and its extract contains active ingredients such as pinene, terpineol and limonene, and has good resistance to various microorganisms.

[0020] Korean cypress is a plant of the Cupressaceae genus, and its extract contains main active ingredients such as alkenes, alcohols, esters, ketones and phenols, and has good antibacterial, antiviral and hair fixing and preventing effects.

[0021] Thyme is a fragrant plant of the Labiatae genus, and its extract is rich in thymol, carvacol, limonene and γ-terpinene, and has good antibacterial, anti-inflammatory and antiviral effects.

[0022] Cupressus is a plant of the Cupressaceae genus, and its leaves contain medicinal active ingredients such as flavonoids and tannins, and have various pharmacological effects such as antibacterial and anti-inflammatory, antitumor, antioxidant, hemostatic and hair growth promoting.

[0023] The present application also provides a preparation method of a bacteriostatic and hair fixing natural plant extract composition containing a metagenome, which specifically comprises the following steps:

[0024] (1) Ayuwi fruit, thyme flowers and cupressus leaves are washed with distilled water, dried and crushed to obtain plant dry powder; a certain amount of plant dry powder is mixed with ethanol solution and soaked for 2-10 h, and then extracted with a Soxhlet extractor at 50-70°C for 5-8 h to collect the extract; the extract is extracted with an ether solvent, concentrated by rotary evaporation and freeze-dried to obtain the corresponding Ayuwi fruit extract, thyme flower extract and cupressus leaf extract;

[0025] (2) taking the whole plant of Cymbopogon citratus, Cinnamomum verum leaves, Melaleuca alternifolia branches and leaves, Sabina koraiensis branches and leaves, washing, drying and crushing to obtain plant dry powder; adding NaCl solution to a certain amount of plant dry powder and soaking for 1-5 h, then performing ultrasonic extraction to obtain a mixed liquid; connecting the mixed liquid to a water vapor distillation device, extracting at 50-70 DEG C for 2-6 h, and collecting the extract; extracting the extract with an ether solvent, and performing rotary evaporation concentration and freeze-drying to obtain the whole plant of Cymbopogon citratus extract, Cinnamomum verum leaf extract, Melaleuca alternifolia branch and leaf extract, and Sabina koraiensis branch and leaf extract;

[0026] (3) taking the fruit extract of A. indica, the whole plant extract of Cymbopogon citratus, the leaf extract of Cinnamomum verum, the branch and leaf extract of Melaleuca alternifolia, the branch and leaf extract of Sabina koraiensis, the flower extract of Thymus vulgaris, and the leaf extract of Biota orientalis by weight, mixing to obtain a plant extract, dissolving in an alcohol solvent and stirring to disperse uniformly, then adding the lysate of Streptococcus thermophilus fermentation product and the fermentation product of Saccharomyces cerevisiae / zinc by weight, stirring and dispersing uniformly to obtain a natural plant extract composition containing a postbiotic.

[0027] Preferably, in step (1), the mass-volume ratio of the plant dry powder of A. indica fruit, Thymus vulgaris flower and Biota orientalis leaf to the ethanol solution is 1g / 4-10mL; the concentration of the ethanol solution is 70-95% by mass fraction; and the ether solvent is one of diethyl ether and petroleum ether.

[0028] Preferably, in step (2), the mass-volume ratio of the plant dry powder of Cymbopogon citratus whole plant, Cinnamomum verum leaf, Melaleuca alternifolia branch and leaf, and Sabina koraiensis branch and leaf to the NaCl solution is 1g / 4-12mL; the concentration of the NaCl solution is 2-9% by mass fraction; the power of ultrasonic extraction is 180-400W, and the ultrasonic time is 30-80min; and the ether solvent is one of diethyl ether and petroleum ether.

[0029] Preferably, in step (3), the alcohol solvent is butanediol; and the mass-volume ratio of the plant extract to butanediol is 1g / 3-12mL.

[0030] The application further provides a use of the natural plant extract composition containing a postbiotic as an additive in the preparation of a hair care product.

[0031] Preferably, the hair care product has the effects of inhibiting bacteria, fixing hair, and preventing hair loss.

[0032] The composition can effectively inhibit microorganism groups such as Pityrosporum ovale and Staphylococcus epidermidis closely related to seborrheic dermatitis, and improve the dandruff proliferation and hair loss problems caused by abnormal proliferation of microorganisms, by the reasonable combination of Aegle marmelos fruit extract, Cymbopogon citratus whole plant extract, Cinnamomum zeylanicum leaf extract, Melaleuca alternifolia branch and leaf extract, and Thymus flower extract, and the synergistic effect of the active ingredients in different plant extracts.

[0033] The composition can effectively strengthen the scalp barrier function and inhibit the expression of inflammatory factors, thereby regulating the scalp microecological balance from the root and relieving symptoms such as seborrheic dermatitis and scalp itching, by adding postbiotic ingredients such as Streptococcus thermophilus fermentation product lysate and yeast / zinc fermentation product.

[0034] Compared with the prior art, the beneficial effects of the present application are:

[0035] The present application realizes the synergistic effect by scientifically compounding Aegle marmelos fruit extract, Cymbopogon citratus whole plant extract, Cinnamomum zeylanicum leaf extract, Melaleuca alternifolia branch and leaf extract, Thuja koraiensis branch and leaf extract, Thymus flower extract, Platycladus orientalis leaf extract, and postbiotics (Streptococcus thermophilus fermentation product lysate and yeast / zinc fermentation product). The experimental results show that the postbiotic-containing antibacterial and hair-fixing natural plant extract composition provided by the present application performs significantly better than each comparative example in terms of antibacterial and anti-hair loss effects. Specifically, compared with the comparative example without Aegle marmelos fruit extract, Thymus flower extract, and Platycladus orientalis leaf extract, the composition of the present application has better performance in maintaining the scalp microecological balance and inhibiting the proliferation of harmful bacteria; compared with the comparative example without postbiotic ingredients, the composition of the present application has significantly improved effect in promoting hair follicle health and reducing hair loss; in addition, the present application further enhances the bioavailability of active ingredients by optimizing the extraction site and preparation method of natural plant extracts, so that the antibacterial and hair-fixing effect of the composition reaches the best level. Therefore, the present application not only provides a safe and efficient natural plant source antibacterial and hair-fixing solution, but also provides a new technical path for developing functional scalp care products. DETAILED DESCRIPTION

[0036] The following examples are further illustrations of the present application, and are not intended to limit the present application.

[0037] Unless otherwise defined, the technical terms used in the following examples have the same meanings as commonly understood by those skilled in the art to which the present application belongs. The test reagents used in the following examples are all conventional biochemical reagents unless otherwise specified; the experimental methods described are all conventional methods unless otherwise specified.

[0038] Example 1

[0039] A bacteriostatic natural plant extract composition containing a postbiotic, comprising, by weight fraction, A. viciae fruit extract 20 parts, C. limonia whole plant extract 25 parts, C. zeylanica leaf extract 30 parts, M. alternifolia branch and leaf extract 30 parts, T. divaricata branch and leaf extract 15 parts, T. vulgaris flower extract 20 parts, T. cuspidata leaf extract 15 parts, S. thermophilus fermentation product lysate 6 parts, yeast / zinc fermentation product 0.1 part.

[0040] The A. viciae fruit extract is prepared as follows: the A. viciae fruit is washed with distilled water, dried, and ground, then 70% ethanol is added at a powder to ethanol solution mass to volume ratio (g / mL) of 1:4, mixed, and soaked for 5 hours, then extracted at 50°C for 5 hours using a Soxhlet extractor, and the extract collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the A. viciae fruit extract.

[0041] The T. vulgaris flower extract is prepared as follows: the T. vulgaris flower is washed with distilled water, dried, and ground, then 75% ethanol is added at a powder to ethanol solution mass to volume ratio (g / mL) of 1:8, mixed, and soaked for 10 hours, then extracted at 70°C for 6 hours using a Soxhlet extractor, and the extract collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the T. vulgaris flower extract.

[0042] The T. cuspidata leaf extract is prepared as follows: the T. cuspidata leaf is washed with distilled water, dried, and ground, then 95% ethanol is added at a powder to ethanol solution mass to volume ratio (g / mL) of 1:10, mixed, and soaked for 8 hours, then extracted at 55°C for 8 hours using a Soxhlet extractor, and the extract collected. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the T. cuspidata leaf extract.

[0043] The C. limonia whole plant extract is prepared as follows: the C. limonia whole plant is washed with distilled water, dried, and ground, then 9% NaCl solution is added at a powder to NaCl solution mass to volume ratio (g / mL) of 1:4, mixed, and soaked for 1 hour, then ultrasonically extracted for 60 minutes using a 320W ultrasonic device to obtain a mixed liquid. The mixed liquid is introduced into a water vapor distillation device, extracted at 50°C for 4 hours, and the extract collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the C. limonia whole plant extract.

[0044] The preparation method of Cinnamomum zeylanicum leaf extract is as follows: after the Cinnamomum zeylanicum leaves are washed with distilled water, dried and crushed, the powder is mixed with 2% NaCl solution at a mass-volume ratio (g / mL) of 1:8, and then soaked for 5 hours. Then, the mixed solution is extracted by 400 W ultrasonic wave for 30 minutes to obtain a mixed solution. The mixed solution is connected to a steam distillation device, and the extraction is carried out at 70°C for 2 hours to collect the extract. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Cinnamomum zeylanicum leaf extract.

[0045] The preparation method of Melaleuca alternifolia branch and leaf extract is as follows: after the Melaleuca alternifolia branch and leaf are washed with distilled water, dried and crushed, the powder is mixed with 5% NaCl solution at a mass-volume ratio (g / mL) of 1:12, and then soaked for 3 hours. Then, the mixed solution is extracted by 250 W ultrasonic wave for 50 minutes to obtain a mixed solution. The mixed solution is connected to a steam distillation device, and the extraction is carried out at 55°C for 2.5 hours to collect the extract. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the Melaleuca alternifolia branch and leaf extract.

[0046] The preparation method of Thuja koraiensis branch and leaf extract is as follows: after the Thuja koraiensis branch and leaf are washed with distilled water, dried and crushed, the powder is mixed with 8% NaCl solution at a mass-volume ratio (g / mL) of 1:9, and then soaked for 4 hours. Then, the mixed solution is extracted by 180 W ultrasonic wave for 80 minutes to obtain a mixed solution. The mixed solution is connected to a steam distillation device, and the extraction is carried out at 60°C for 6 hours to collect the extract. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the Thuja koraiensis branch and leaf extract.

[0047] The plant extract is obtained by mixing Aegle marmelos fruit extract, Cymbopogon citratus whole plant extract, Cinnamomum zeylanicum leaf extract, Melaleuca alternifolia branch and leaf extract, Thuja koraiensis branch and leaf extract, Thymus vulgaris flower extract, and Biota orientalis leaf extract in a weight ratio of 40:15:10:10:30:5:20, respectively. Then, the plant extract is dissolved in butanediol at a mass-volume ratio (g / mL) of 1:3, and stirred and dispersed uniformly. Then, 6 parts of Streptococcus thermophilus fermentation product lysate and 0.1 part of yeast / zinc fermentation product are added, and stirred and dispersed uniformly to obtain a natural plant extract mixture containing metacommensal.

[0048] Example 2

[0049] A metacommensal-containing antibacterial and hair-fixing natural plant extract composition comprises Aegle marmelos fruit extract 40 parts, Cymbopogon citratus whole plant extract 15 parts, Cinnamomum zeylanicum leaf extract 10 parts, Melaleuca alternifolia branch and leaf extract 10 parts, Thuja koraiensis branch and leaf extract 30 parts, Thymus vulgaris flower extract 5 parts, Biota orientalis leaf extract 20 parts, Streptococcus thermophilus fermentation product lysate 0.1 part, and yeast / zinc fermentation product 5 parts.

[0050] The preparation method of the extract of the fruit of Aegle marmelos is as follows: the fruit of Aegle marmelos is washed with distilled water, dried, and crushed, and then 75% ethanol is added at a mass-volume ratio (g / mL) of 1:10, and the mixture is soaked for 2 hours, and then extracted at 60°C for 8 hours using a Soxhlet extractor, and the extract is collected. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of the fruit of Aegle marmelos.

[0051] The preparation method of the extract of the flower of Thymus vulgaris is as follows: the flower of Thymus vulgaris is washed with distilled water, dried, and crushed, and then 95% ethanol is added at a mass-volume ratio (g / mL) of 1:6, and the mixture is soaked for 4 hours, and then extracted at 55°C for 5 hours using a Soxhlet extractor, and the extract is collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of the flower of Thymus vulgaris.

[0052] The preparation method of the extract of the leaf of Platycladus orientalis is as follows: the leaf of Platycladus orientalis is washed with distilled water, dried, and crushed, and then 70% ethanol is added at a mass-volume ratio (g / mL) of 1:4, and the mixture is soaked for 10 hours, and then extracted at 70°C for 6 hours using a Soxhlet extractor, and the extract is collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of the leaf of Platycladus orientalis.

[0053] The preparation method of the extract of the whole plant of Cymbopogon citratus is as follows: the whole plant of Cymbopogon citratus is washed with distilled water, dried, and crushed, and then 9% NaCl solution is added at a mass-volume ratio (g / mL) of 1:8, and the mixture is soaked for 1 hour, and then ultrasonic extraction is performed for 80 minutes using a 280W ultrasonic wave to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extract is collected after being extracted at 55°C for 3 hours. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of the whole plant of Cymbopogon citratus.

[0054] The preparation method of the extract of the leaf of Cinnamomum verum is as follows: the leaf of Cinnamomum verum is washed with distilled water, dried, and crushed, and then 5% NaCl solution is added at a mass-volume ratio (g / mL) of 1:9, and the mixture is soaked for 3 hours, and then ultrasonic extraction is performed for 60 minutes using a 200W ultrasonic wave to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extract is collected after being extracted at 65°C for 3 hours. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of the leaf of Cinnamomum verum.

[0055] The preparation method of the Melaleuca alternifolia branch and leaf extract is as follows: after the Melaleuca alternifolia branch and leaf is washed with distilled water, dried and crushed, 8% NaCl solution is added in a mass-volume ratio (g / mL) of 1:10 of the powder to the NaCl solution, and the mixture is soaked for 4 hours, and then extracted with 280W ultrasonic waves for 40 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extraction liquid is collected after being extracted at 70°C for 4 hours. The extraction liquid is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the Melaleuca alternifolia branch and leaf extract.

[0056] The preparation method of the Thuja koraiensis branch and leaf extract is as follows: after the Thuja koraiensis branch and leaf is washed with distilled water, dried and crushed, 6% NaCl solution is added in a mass-volume ratio (g / mL) of 1:6 of the powder to the NaCl solution, and the mixture is soaked for 5 hours, and then extracted with 320W ultrasonic waves for 55 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extraction liquid is collected after being extracted at 55°C for 6 hours. The extraction liquid is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Thuja koraiensis branch and leaf extract.

[0057] The plant extract is obtained by mixing, in parts by weight, the Aegle marmelos fruit extract, the Cymbopogon citratus whole plant extract, the Cinnamomum verum leaf extract, the Melaleuca alternifolia branch and leaf extract, the Thuja koraiensis branch and leaf extract, the Thymus flower extract, and the Platycladus orientalis leaf extract. The plant extract is dissolved in butanediol in a mass-volume ratio (g / mL) of 1:8 of the plant extract to butanediol, and then 0.1 parts of the Streptococcus thermophilus fermentation product lysate and 5 parts of the yeast / zinc fermentation product are added and uniformly dispersed by stirring to obtain a natural plant extract mixture containing a postbiotic.

[0058] Example 3

[0059] A postbiotic-containing antibacterial and hair growth-promoting natural plant extract composition includes, in parts by weight, 38 parts of Aegle marmelos fruit extract, 40 parts of Cymbopogon citratus whole plant extract, 20 parts of Cinnamomum verum leaf extract, 25 parts of Melaleuca alternifolia branch and leaf extract, 28 parts of Thuja koraiensis branch and leaf extract, 15 parts of Thymus flower extract, 12 parts of Platycladus orientalis leaf extract, 4 parts of Streptococcus thermophilus fermentation product lysate, and 2 parts of yeast / zinc fermentation product.

[0060] The preparation method of the Aegle marmelos fruit extract is as follows: after the Aegle marmelos fruit is washed with distilled water, dried and crushed, 70% ethanol is added in a mass-volume ratio (g / mL) of 1:6 of the powder to the ethanol solution, and the mixture is soaked for 6 hours, and then extracted at 65°C for 6 hours using a Soxhlet extractor to collect the extraction liquid. The extraction liquid is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Aegle marmelos fruit extract.

[0061] The preparation method of the thyme flower extract is as follows: after the thyme flowers are washed with distilled water, dried, and crushed, the powder is mixed with an ethanol solution at a mass-volume ratio (g / mL) of 1:4, 95% ethanol is added and soaked for 6 hours, and then extracted at 70°C for 6.5 hours by a Soxhlet extractor to collect the extract. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the thyme flower extract.

[0062] The preparation method of the cypress leaf extract is as follows: after the cypress leaves are washed with distilled water, dried, and crushed, the powder is mixed with an ethanol solution at a mass-volume ratio (g / mL) of 1:8, 75% ethanol is added and soaked for 5.5 hours, and then extracted at 55°C for 8 hours by a Soxhlet extractor to collect the extract. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the cypress leaf extract.

[0063] The preparation method of the lemon grass whole plant extract is as follows: after the lemon grass whole plant is washed with distilled water, dried, and crushed, the powder is mixed with a NaCl solution at a mass-volume ratio (g / mL) of 1:4, 8.5% NaCl solution is added and soaked for 5 hours, and then extracted by ultrasonic waves at 200 W for 40 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, extracted at 60°C for 4 hours to collect the extract. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the lemon grass whole plant extract.

[0064] The preparation method of the Ceylon cinnamon leaf extract is as follows: after the Ceylon cinnamon leaves are washed with distilled water, dried, and crushed, the powder is mixed with a NaCl solution at a mass-volume ratio (g / mL) of 1:10, 8% NaCl solution is added and soaked for 5 hours, and then extracted by ultrasonic waves at 280 W for 45 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, extracted at 70°C for 3.5 hours to collect the extract. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the Ceylon cinnamon leaf extract.

[0065] The preparation method of the Melaleuca alternifolia branch leaf extract is as follows: after the Melaleuca alternifolia branch leaves are washed with distilled water, dried, and crushed, the powder is mixed with a NaCl solution at a mass-volume ratio (g / mL) of 1:12, 4% NaCl solution is added and soaked for 1.5 hours, and then extracted by ultrasonic waves at 400 W for 60 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, extracted at 55°C for 5 hours to collect the extract. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Melaleuca alternifolia branch leaf extract.

[0066] The preparation method of the Thuja koraiensis Nakai branch and leaf extract is as follows: after the Thuja koraiensis Nakai branch and leaf is washed with distilled water, dried and crushed, the powder is mixed with 8% NaCl solution at a mass-volume ratio (g / mL) of 1:8, soaked for 2 hours, and then extracted with 250W ultrasonic wave for 65 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extraction liquid is collected after being extracted at 65°C for 4 hours. The extraction liquid is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Thuja koraiensis Nakai branch and leaf extract.

[0067] The plant extract is obtained by mixing, by weight fraction, the Aegle marmelos Corr. fruit extract, the Cymbopogon citratus DC. whole plant extract, the Cinnamomum verum leaf extract, the Melaleuca alternifolia branch and leaf extract, the Thuja koraiensis Nakai branch and leaf extract, the Thymus vulgaris flower extract, and the Biota orientalis leaf extract. The plant extract is dissolved in butanediol at a mass-volume ratio (g / mL) of 1:12, stirred and dispersed uniformly, 4 parts of Streptococcus thermophilus fermentation product lysate and 2 parts of yeast / zinc fermentation product are then added, and stirred and dispersed uniformly to obtain a natural plant extract mixture containing metagenomics.

[0068] Example 4

[0069] A metagenomics-containing antibacterial and hair growth-promoting natural plant extract composition comprises, by weight fraction, 32 parts of Aegle marmelos Corr. fruit extract, 38 parts of Cymbopogon citratus DC. whole plant extract, 25 parts of Cinnamomum verum leaf extract, 20 parts of Melaleuca alternifolia branch and leaf extract, 25 parts of Thuja koraiensis Nakai branch and leaf extract, 10 parts of Thymus vulgaris flower extract, 15 parts of Biota orientalis leaf extract, 2 parts of Streptococcus thermophilus fermentation product lysate, and 4.5 parts of yeast / zinc fermentation product.

[0070] The preparation method of the Aegle marmelos Corr. fruit extract is as follows: after the Aegle marmelos Corr. fruit is washed with distilled water, dried and crushed, the powder is mixed with 75% ethanol at a mass-volume ratio (g / mL) of 1:10, soaked for 3 hours, and then extracted at 60°C for 8 hours with a Soxhlet extractor to collect the extraction liquid. The extraction liquid is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Aegle marmelos Corr. fruit extract.

[0071] The preparation method of the Thymus vulgaris flower extract is as follows: after the Thymus vulgaris flower is washed with distilled water, dried and crushed, the powder is mixed with 75% ethanol at a mass-volume ratio (g / mL) of 1:7, soaked for 5 hours, and then extracted at 55°C for 6 hours with a Soxhlet extractor to collect the extraction liquid. The extraction liquid is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the Thymus vulgaris flower extract.

[0072] The preparation method of the extract of Platycladus orientalis is as follows: after the Platycladus orientalis is washed with distilled water, dried and crushed, the powder is mixed with 70% ethanol solution at a mass-volume ratio (g / mL) of 1:5, and then soaked for 8 hours. The mixture is extracted with a Soxhlet extractor at 60°C for 7 hours, and the extract is collected. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of Platycladus orientalis.

[0073] The preparation method of the extract of Cymbopogon citratus is as follows: after the Cymbopogon citratus is washed with distilled water, dried and crushed, the powder is mixed with 3% NaCl solution at a mass-volume ratio (g / mL) of 1:12, and then soaked for 2 hours. The mixture is extracted with an ultrasonic wave of 240W for 50 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extract is collected after being extracted at 60°C for 4 hours. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of Cymbopogon citratus.

[0074] The preparation method of the extract of Cinnamomum verum is as follows: after the Cinnamomum verum is washed with distilled water, dried and crushed, the powder is mixed with 5% NaCl solution at a mass-volume ratio (g / mL) of 1:6, and then soaked for 2.5 hours. The mixture is extracted with an ultrasonic wave of 320W for 30 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extract is collected after being extracted at 65°C for 5 hours. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of Cinnamomum verum.

[0075] The preparation method of the extract of Melaleuca alternifolia is as follows: after the Melaleuca alternifolia is washed with distilled water, dried and crushed, the powder is mixed with 7% NaCl solution at a mass-volume ratio (g / mL) of 1:9, and then soaked for 3 hours. The mixture is extracted with an ultrasonic wave of 220W for 80 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extract is collected after being extracted at 60°C for 3 hours. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of Melaleuca alternifolia.

[0076] The preparation method of the extract of Thuja koraiensis is as follows: after the Thuja koraiensis is washed with distilled water, dried and crushed, the powder is mixed with 6% NaCl solution at a mass-volume ratio (g / mL) of 1:10, and then soaked for 3 hours. The mixture is extracted with an ultrasonic wave of 300W for 40 minutes to obtain a mixed liquid. The mixed liquid is connected to a water vapor distillation device, and the extract is collected after being extracted at 70°C for 2 hours. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of Thuja koraiensis.

[0077] The plant extracts of A. indica fruit extract, C. flexuosa whole plant extract, C. zeylanica leaf extract, M. alternifolia branch and leaf extract, T. divaricata branch and leaf extract, T. vulgaris flower extract and T. divaricata leaf extract were mixed to obtain a plant extract, which was dissolved in butanediol at a mass-volume ratio (g / mL) of 1:10, and then stirred and dispersed uniformly. Then, 2 parts of S. thermophilus fermentation product lysate and 4.5 parts of yeast / zinc fermentation product were added, and stirred and dispersed uniformly to obtain a natural plant extract mixture containing postbiotics.

[0078] Example 5

[0079] A natural plant extract composition containing postbiotics for inhibiting bacteria and fixing hair, comprising, by weight fraction, 36 parts of A. indica fruit extract, 35 parts of C. flexuosa whole plant extract, 23 parts of C. zeylanica leaf extract, 22 parts of M. alternifolia branch and leaf extract, 28 parts of T. divaricata branch and leaf extract, 16 parts of T. vulgaris flower extract, 18 parts of T. divaricata leaf extract, 3 parts of S. thermophilus fermentation product lysate, and 2.5 parts of yeast / zinc fermentation product.

[0080] The A. indica fruit extract was prepared as follows: The A. indica fruit was washed with distilled water, dried, and ground into powder. Then, 95% ethanol was added to the powder at a mass-volume ratio (g / mL) of 1:8, and the mixture was soaked for 6 hours. The mixture was then extracted with a Soxhlet extractor at 70°C for 5.5 hours, and the extract was collected. The extract was extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the A. indica fruit extract.

[0081] The T. vulgaris flower extract was prepared as follows: The T. vulgaris flower was washed with distilled water, dried, and ground into powder. Then, 70% ethanol was added to the powder at a mass-volume ratio (g / mL) of 1:10, and the mixture was soaked for 2 hours. The mixture was then extracted with a Soxhlet extractor at 55°C for 8 hours, and the extract was collected. The extract was extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the T. vulgaris flower extract.

[0082] The T. divaricata leaf extract was prepared as follows: The T. divaricata leaf was washed with distilled water, dried, and ground into powder. Then, 95% ethanol was added to the powder at a mass-volume ratio (g / mL) of 1:6, and the mixture was soaked for 4 hours. The mixture was then extracted with a Soxhlet extractor at 60°C for 5 hours, and the extract was collected. The extract was extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the T. divaricata leaf extract.

[0083] The preparation method of lemongrass whole plant extract is as follows: The whole lemongrass plant was washed with distilled water, dried, and pulverized. Then, 2% NaCl solution was added at a powder-to-NaCl solution mass-to-volume ratio (g / mL) of 1:12. After mixing and soaking for 2 h, the mixture was ultrasonically extracted for 35 min using 300W ultrasound to obtain a mixed solution. The mixed solution was then placed in a steam distillation apparatus and extracted at 65℃ for 3 h, after which the extract was collected. The extract was extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the lemongrass whole plant extract.

[0084] The preparation method of Ceylon cinnamon leaf extract is as follows: Ceylon cinnamon leaves are washed with distilled water, dried, and pulverized. Then, 5% NaCl solution is added at a powder-to-NaCl solution mass-to-volume ratio (g / mL) of 1:6, and the mixture is soaked for 3 h. The mixture is then ultrasonically extracted at 320 W for 50 min to obtain a mixed solution. This mixed solution is then placed in a steam distillation apparatus and extracted at 50℃ for 4 h, after which the extract is collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Ceylon cinnamon leaf extract.

[0085] The preparation method of Melaleuca alternifolia leaf and branch extract is as follows: Melaleuca alternifolia leaves and branches are washed with distilled water, dried, and pulverized. A 6% NaCl solution is added at a powder-to-NaCl solution mass-to-volume ratio (g / mL) of 1:8, and the mixture is soaked for 3 h. The mixture is then ultrasonically extracted at 240 W for 80 min to obtain a mixed liquid. This mixed liquid is then placed in a steam distillation apparatus and extracted at 70℃ for 4 h, after which the extract is collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the Melaleuca alternifolia leaf and branch extract.

[0086] The preparation method of the extract of Korean cypress branches and leaves is as follows: Korean cypress branches and leaves are washed with distilled water, dried, and pulverized. A 9% NaCl solution is added at a powder-to-NaCl solution mass-to-volume ratio (g / mL) of 1:10, and the mixture is soaked for 4 h. Then, it is ultrasonically extracted for 35 min using 300W ultrasound to obtain a mixed liquid. The mixed liquid is then placed in a steam distillation apparatus and extracted at 70℃ for 6 h, after which the extract is collected. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the Korean cypress branch and leaf extract.

[0087] The following extracts were taken by weight: Ayurvedic fruit extract, lemongrass whole plant extract, Ceylon cinnamon leaf extract, Melaleuca alternifolia branch and leaf extract, Korean cypress branch and leaf extract, thyme flower extract, and Platycladus orientalis leaf extract. They were mixed to obtain plant extracts. The plant extracts were added to butylene glycol at a mass-to-volume ratio (g / mL) of 1:8. After dissolving and stirring to disperse evenly, 3 parts of Streptococcus thermophilus fermentation product lysate and 2.5 parts of yeast / zinc fermentation product were added. After stirring and dispersing evenly, a natural plant extract composition containing metageners was obtained.

[0088] Comparative Example 1

[0089] The difference between the present comparative example and Example 5 is that Comparative Example 1 does not contain the extracts of A. indica fruit, the extract of Thymus flower and the extract of Platycladus orientalis, and the rest is the same as Example 5. The bacteriostatic and hair-fixing natural plant extract composition containing a secondary metabolite of Comparative Example 1 contains, by weight fraction, 35 parts of the extract of Cymbopogon citratus, 23 parts of the extract of Cinnamomum zeylanicum, 22 parts of the extract of Melaleuca alternifolia, 18 parts of the extract of Platycladus orientalis, 3 parts of the lysate of Streptococcus thermophilus fermentation product, and 2.5 parts of the fermentation product of Saccharomyces / Zinc.

[0090] Comparative Example 2

[0091] The difference between the present comparative example and Example 5 is that Comparative Example 2 does not contain the lysate of Streptococcus thermophilus fermentation product and the fermentation product of Saccharomyces / Zinc, and the rest is the same as Example 5. The bacteriostatic and hair-fixing natural plant extract composition of Comparative Example 2 contains, by weight fraction, 36 parts of the extract of A. indica fruit, 35 parts of the extract of Cymbopogon citratus, 23 parts of the extract of Cinnamomum zeylanicum, 22 parts of the extract of Melaleuca alternifolia, 28 parts of the extract of Thuja koraiensis, 16 parts of the extract of Thymus flower, and 18 parts of the extract of Platycladus orientalis.

[0092] Comparative Example 3

[0093] The difference between the present comparative example and Example 5 is that Comparative Example 3 uses different parts of natural plant extracts, and the rest is the same as Example 5. The bacteriostatic and hair-fixing natural plant extract composition containing a secondary metabolite of Comparative Example 3 contains, by weight fraction, 36 parts of the extract of A. indica branches and leaves, 35 parts of the extract of Cymbopogon citratus, 23 parts of the extract of Cinnamomum zeylanicum bark, 22 parts of the extract of Melaleuca alternifolia flower, 28 parts of the extract of Thuja koraiensis seeds, 16 parts of the extract of Thymus leaves, 18 parts of the extract of Platycladus seeds, 3 parts of the lysate of Streptococcus thermophilus fermentation product, and 2.5 parts of the fermentation product of Saccharomyces / Zinc.

[0094] Comparative Example 4

[0095] The difference between the present comparative example and Example 5 is that Comparative Example 4 uses different preparation methods for the extract of A. indica fruit, the extract of Cymbopogon citratus, the extract of Platycladus orientalis, and the extract of Melaleuca alternifolia flower, and the rest is the same as Example 5.

[0096] The preparation method of the extract of A. indica fruit in Comparative Example 4 is as follows: the fruit of A. indica is washed with distilled water, dried, and ground into powder. Then, 95% ethanol is added to the powder at a mass-volume ratio (g / mL) of 1:2, and the mixture is extracted with a Soxhlet extractor at 85°C for 4 hours. The extract is collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the extract of A. indica fruit.

[0097] The preparation method of the lemon grass whole plant extract in Comparative Example 4 is as follows: the lemon grass whole plant is washed with distilled water, dried, and ground, and then 2% NaCl solution is added to the powder at a mass-volume ratio (g / mL) of 1:20, and the mixture is soaked for 2 h. The mixture is then introduced into a water vapor distillation device, and the extraction is carried out at 40°C for 1.5 h, and the extract is collected. The extract is extracted with petroleum ether, concentrated by rotary evaporation, and freeze-dried to obtain the lemon grass whole plant extract.

[0098] The preparation method of the cypress leaf extract in Comparative Example 4 is as follows: the cypress leaf is washed with distilled water, dried, and ground, and then ethanol solution is added to the powder at a mass-volume ratio (g / mL) of 1:15, and the mixture is extracted at 80°C for 10 h using a Soxhlet extractor, and the extract is collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the cypress leaf extract.

[0099] The preparation method of the white mahogany branch and leaf extract in Comparative Example 4 is as follows: the white mahogany branch and leaf is washed with distilled water, dried, and ground, and then 12% NaCl solution is added to the powder at a mass-volume ratio (g / mL) of 1:3, and the mixture is introduced into a water vapor distillation device, and the extraction is carried out at 75°C for 8 h, and the extract is collected. The extract is extracted with diethyl ether, concentrated by rotary evaporation, and freeze-dried to obtain the white mahogany branch and leaf extract.

[0100] The following is a description of the antibacterial efficacy and hair fixing efficacy tests of the compositions of Examples 1-5 and Comparative Examples 1-4.

[0101] (1) Antibacterial efficacy test

[0102] The main microbial species causing seborrheic alopecia is Malassezia furfur and Staphylococcus epidermidis.

[0103] The Oxford cup method is used for the related antibacterial efficacy test. The inhibition of the test sample on Malassezia furfur (ATCC44344) and Staphylococcus epidermidis (ATCC25923) is determined by the inhibition zone test. The larger the inhibition zone, the stronger the antibacterial ability of the sample.

[0104] Test sample: the compositions prepared in Examples 1-5 and Comparative Examples 1-4 are diluted with dimethyl sulfoxide to a mass fraction of 5% for the test sample.

[0105] Malassezia furfur test method: under sterile conditions, the modified Dixon agar was used to culture the resuscitated and activated Malassezia furfur at 32°C. A sterile inoculating loop was used to pick the activated strain to prepare a bacterial suspension, and sterile saline was used to adjust the concentration of the bacterial solution to 1 x 10 6 0.1 mL of the bacterial solution was spread on a modified Dixon agar plate, and an Oxford cup was placed on the surface of the culture medium using sterile forceps. After gentle pressing, 0.1 mL of the sample to be tested was added to the small tube, which was then placed in a 32°C incubator for 72 hours. After removal, the inhibition zone diameter was observed and measured using a vernier caliper. Three replicates were set for each sample group, and sterile saline was used as the negative control group.

[0106] Staphylococcus epidermidis test method: under sterile conditions, the Staphylococcus epidermidis was resuscitated and activated using a tryptone soya broth agar (TSA) culture medium. A sterile inoculating loop was used to pick the activated strain to prepare a bacterial suspension, and sterile saline was used to adjust the concentration of the bacterial solution to 1 x 10 6 0.1 mL of the bacterial solution was spread on a TSA plate, and an Oxford cup was placed on the surface of the culture medium using sterile forceps. After gentle pressing, 0.1 mL of the sample to be tested was added to the small tube, which was then placed in a 37°C incubator for 24 hours. After removal, the inhibition zone diameter was observed and measured using a vernier caliper. Three replicates were set for each sample group, and sterile saline was used as the negative control.

[0107] Inhibition effect evaluation criteria: the inhibition effect of the sample was evaluated based on the size of the inhibition zone. The larger the inhibition zone, the stronger the inhibition ability of the sample. The actual inhibition zone diameter of the sample group = the inhibition zone diameter of the sample group - the inhibition zone diameter of the control group. An actual inhibition zone diameter > 20 mm is high efficiency; an inhibition zone diameter of 15-19 mm is medium efficiency; an inhibition zone diameter of 10-14 mm is low efficiency, and an inhibition zone diameter < 10 mm is ineffective. The inhibition effects of the examples and comparative examples are shown in Table 1.

[0108] Table 1 Inhibition effect of postbiotic-containing antibacterial and hair growth natural plant extract compositions on Malassezia furfur and Staphylococcus epidermidis

[0109] As can be seen from Table 1, the compositions of Examples 1-5 of the present application have strong bacteriostatic effects on Malassezia furfur and Staphylococcus epidermidis, and the bacteriostatic circle diameter of Example 5 is the largest, indicating that the bacteriostatic effect of Example 5 is the strongest. Compared with Example 5, the bacteriostatic circles of the compositions of Comparative Examples 1-4 on the two test bacteria are reduced to different degrees, indicating that the compositions of Example 5 have certain synergies and can effectively inhibit Malassezia furfur and Staphylococcus epidermidis closely related to seborrheic alopecia. Compared with Example 5, the antibacterial performance of Comparative Example 1 is significantly reduced because it does not contain A. villosa fruit extract, Thymus flower extract and Platycladus orientalis leaf extract; the antibacterial performance of Comparative Example 2 is slightly reduced, indicating that the absence of Streptococcus thermophilus fermentation product lysate and yeast / zinc fermentation product has little effect on the antibacterial performance of the composition; the antibacterial performance of Comparative Example 3 is significantly reduced, indicating that the selection of different plant extract parts has a great influence on the antibacterial performance of the composition; the antibacterial performance of Comparative Example 4 is also significantly reduced because different preparation methods are selected for some plants.

[0110] (2) Hair growth (anti-hair loss) efficacy test

[0111] The natural plant extract compositions prepared from Example 5 and Comparative Examples 1-4 are used for hair growth efficacy test. The bacteriostatic hair growth natural plant extract compositions prepared from Example 5 and Comparative Examples 1-4 are mixed with shampoo base to prepare shampoo. The shampoo contains the following components in mass fraction: bacteriostatic hair growth natural plant extract composition containing postbiotic 9%, deionized water 54%, butanediol 20%, hexanediol 5%, silicone oil 5%, cocamide methyl 1.5%, sodium lauryl polyether sulfate 5% and methyl p-hydroxybenzoate 0.5%.

[0112] Test method: The shampoo containing the bacteriostatic hair growth compositions of Example 5 and Comparative Examples 1-4 is tested for anti-hair loss experiment according to the Cosmetics Safety Technical Specification-Anti-hair loss efficacy test method. 30 volunteers with the phenomenon of frequent hair loss and mild sparse hair are collected, half of them are male and half of them are female, and their age is 20-60 years old. The volunteers have not performed hair dyeing, hair perming, styling, etc. in the past month, and have not taken or used any drug affecting hair growth in the past two months. The volunteers are randomly divided into 5 groups, 6 people in each group (half male and half female), and the bacteriostatic hair growth compositions of Example 5 and Comparative Examples 1-4 are used for 28 days. During the use, the volunteers wash their hair every 2 days according to their daily habits, and continuously use for 28 days. Before and after using the test sample, the volunteers' hair is combed by 60 times of combing method, the number of hair loss is counted and recorded. The hair loss count of each group of volunteers is counted and the average value is taken, and the results are shown in Table 2.

[0113] Table 2 Anti-hair loss effect of bacteriostatic hair growth natural plant extract composition containing postbiotic

[0114] As can be seen from Table 2, the postbiotic-containing antibacterial hair growth natural plant extract composition prepared in Example 5 has a significantly reduced amount of hair loss after 28 days of use, indicating that the composition has good hair growth (anti-hair loss) effect. Compared with Example 5, the hair growth effects of the corresponding compositions of Comparative Examples 1-4 are all significantly reduced, indicating that the different raw material components of the composition corresponding to Example 5 have a synergistic effect, which can effectively improve the problem of hair loss. The hair growth effect of Example 5 is better than that of Comparative Example 1, indicating that the selected combination of natural plant species in Example 5 is reasonable and scientific, and the active ingredients in different extracts can have a synergistic effect. The hair growth effect of Example 5 is better than that of Comparative Example 2, indicating that the postbiotic as a raw material can effectively regulate the scalp microecology and fundamentally improve the scalp barrier to improve the hair growth effect of the composition. The hair growth effect of Example 5 is better than that of Comparative Example 3, indicating that the active ingredients of different plant parts have a significant effect on the hair growth effect of the composition, and by optimizing and selecting the active extracts of different parts of natural plants, the hair growth effect of the composite plant extract can be enhanced. The hair growth effect of Example 5 is better than that of Comparative Example 4, indicating that the difference in preparation method of different plant extracts can significantly affect the actual hair growth effect of the composition.

[0115] The above detailed description is a specific description of the embodiments of the present application, and the embodiments are not used to limit the patent scope of the present application. Any equivalent implementation or modification that does not deviate from the present application shall be included in the patent scope of the present application.

Claims

1. A bacteriostatic, solid natural plant extract composition containing a postbiotic, characterized in that, The composition comprises natural plant extracts and postbiotics; the natural plant extracts are one or more of Aegle marmelos extract, Cymbopogon citratus extract, Cinnamomum zeylanicum extract, Melaleuca alternifolia extract, Thuja koraiensis extract, Thymus vulgaris extract and Platycladus orientalis extract; the postbiotics are one or more of Streptococcus thermophilus fermentation product lysate and Saccharomyces / Zinc fermentation product.

2. The probiotic-containing bacteriostatic, solid natural plant extract composition according to claim 1, characterized in that, The natural plant extracts comprise the following raw materials in parts by weight: Aegle marmelos fruit extract 20-40 parts, Cymbopogon citratus whole plant extract 15-40 parts, Cinnamomum zeylanicum leaf extract 10-30 parts, Melaleuca alternifolia branch and leaf extract 10-30 parts, Thuja koraiensis branch and leaf extract 15-30 parts, Thymus vulgaris flower extract 5-20 parts, and Platycladus orientalis leaf extract 5-20 parts.

3. The probiotic-containing bacteriostatic, solid natural plant extract composition according to claim 1, characterized in that, The postbiotics comprise the following raw materials in parts by weight: Streptococcus thermophilus fermentation product lysate 0.1-6 parts and Saccharomyces / Zinc fermentation product 0.1-5 parts.

4. The probiotic-containing bacteriostatic, solid natural plant extract composition according to claim 1, characterized in that, The composition comprises the following raw materials in parts by weight: Aegle marmelos fruit extract 20-40 parts, Cymbopogon citratus whole plant extract 15-40 parts, Cinnamomum zeylanicum leaf extract 10-30 parts, Melaleuca alternifolia branch and leaf extract 10-30 parts, Thuja koraiensis branch and leaf extract 15-30 parts, Thymus vulgaris flower extract 5-20 parts, Platycladus orientalis leaf extract 5-20 parts, Streptococcus thermophilus fermentation product lysate 0.1-6 parts, and Saccharomyces / Zinc fermentation product 0.1-5 parts.

5. A process for the preparation of a bacteriostatic, pro-biotic containing natural plant extract composition of claim 4, characterized in that, The method comprises the following steps: (1) Aegle marmelos fruit, Thymus vulgaris flower and Platycladus orientalis leaf are washed with distilled water, dried, and ground into plant dry powder; a certain amount of plant dry powder is mixed with ethanol solution and soaked for 2-10 hours, and then extracted with a Soxhlet extractor at 50-70°C for 5-8 hours to collect the extract; the extract is extracted with an ether solvent, concentrated by rotary evaporation, and freeze-dried to obtain Aegle marmelos fruit extract, Thymus vulgaris flower extract and Platycladus orientalis leaf extract; (2) Cymbopogon citratus whole plant, Cinnamomum zeylanicum leaf, Melaleuca alternifolia branch and leaf, and Thuja koraiensis branch and leaf are washed with distilled water, dried, and ground into plant dry powder; a certain amount of plant dry powder is added with NaCl solution and soaked for 1-5 hours, and then ultrasonic extraction is performed to obtain a mixed liquid; the mixed liquid is introduced into a water vapor distillation device, extracted at 50-70°C for 2-6 hours, and then the extract is collected; the extract is extracted with an ether solvent, concentrated by rotary evaporation, and freeze-dried to obtain Cymbopogon citratus whole plant extract, Cinnamomum zeylanicum leaf extract, Melaleuca alternifolia branch and leaf extract, and Thuja koraiensis branch and leaf extract; (3) Aegle marmelos fruit extract, Cymbopogon citratus whole plant extract, Cinnamomum zeylanicum leaf extract, Melaleuca alternifolia branch and leaf extract, Thuja koraiensis branch and leaf extract, Thymus vulgaris flower extract and Platycladus orientalis leaf extract are taken in parts by weight, mixed, dissolved in an alcohol solvent, and stirred and dispersed uniformly; Streptococcus thermophilus fermentation product lysate and Saccharomyces / Zinc fermentation product are added in parts by weight, stirred and dispersed uniformly to obtain a natural plant extract composition containing postbiotics.

6. The production method according to claim 5, wherein In step (1), the mass-to-volume ratio of the dried plant powder of Ashoka fruit, thyme flower, and Platycladus orientalis leaf to the ethanol solution is 1g / 4~10mL; the concentration of the ethanol solution is 70-95% by mass.

7. The preparation method according to claim 5, characterized in that, In step (2), the mass-to-volume ratio of the dried plant powder of lemongrass whole plant, Ceylon cinnamon leaf, Melaleuca alternifolia branches and leaves, and Korean cypress branches and leaves to NaCl solution is 1g / 4~12mL; the concentration of NaCl solution is 2-9% by mass; the ultrasonic extraction power is 180-400W, and the ultrasonic time is 30-80min.

8. The preparation method according to claim 5, characterized in that, In step (3), the alcohol solvent is butanediol; the mass-to-volume ratio of the plant extract to butanediol is 1g / 3~12mL.

9. The use of the composition of antibacterial and hair-strengthening natural plant extracts containing post-genetic agents as described in any one of claims 1-4 as an additive in the preparation of hair care products.

10. Use according to claim 9, characterized in that, The hair care products described herein have antibacterial, hair-strengthening, and hair-preventing effects.

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