Thuja sutchuenensis essential oil for treating scalds, soothing nerves and helping sleep and preparation method thereof

Abstract

The invention discloses a thuja sutchuenensis essential oil and platycladus orientalis extract for treating scalds, soothing nerves and helping sleep and a preparation method thereof, and relates to the technical field of thuja sutchuenensis essential oil and platycladus orientalis extract preparation, and the preparation method comprises the following preparation steps: S1, raw material preparation: selecting branches and trunks of thuja sutchuenensis, removing impurities and pest and disease parts, cleaning, and airing for later use; s2, crushing: crushing the cleaned branches and trunks to obtain thuja sutchuenensis powder; s3, soaking: soaking the thuja sutchuenensis powder with a solvent; s4, extraction: performing ultrasonic-assisted extraction on the soaked thuja sutchuenensis powder to obtain a thuja sutchuenensis extracting solution; s5, filtering and concentrating: after ultrasonic extraction is completed, filtering the thuja sutchuenensis extracting solution. The thuja sutchuenensis essential oil and platycladus orientalis extract disclosed by the invention shows remarkable effects in the aspects of treating scalds, relieving itching, soothing the nerves, aiding sleep and relieving anxiety, and compared with the traditional technology, the thuja sutchuenensis essential oil and platycladus orientalis extract disclosed by the invention has higher purity and clearer efficacy, and the thuja sutchuenensis essential oil and platycladus orientalis extract is simple in preparation process, low in cost and wide in prospect and application potential.

Description

Technical Field

[0001] This invention relates to the field of preparation technology of Chinese cypress essential oil and Chinese cypress extract, specifically to a Chinese cypress essential oil for treating burns and for calming the nerves and promoting sleep, and its preparation method. Background Technology

[0002] *Thuja orientalis*, commonly known as cliff cypress, is an evergreen shrub or tree belonging to the genus *Thuja* in the family Cupressaceae. It has dense branches, flattened twigs, and scale-like leaves. The scale-like leaves growing in the center of the twigs are oblique-ovate, while those at the tips are boat-shaped, and both surfaces are green. It is monoecious with separate male and female branches. The flowers, called "cliff cypress," grow at the branch tips. Male flowers are round, pale red or pale yellow; female flowers are very small, green or purplish. Mature cones are solitary, ovoid or oblong. *Thuja orientalis* gets its name from its growth on cliffs. Cupressaceae plants possess a wide range of biological activities, including hemostasis, antitussive and expectorant effects, bronchodilator properties, antibacterial activity, antitumor effects, and antioxidant properties. They are widely applicable in various fields such as medicine, cosmetics, food, aromatherapy, fragrance, and chemical additives.

[0003] Thuja orientalis essential oil is an important extract of thuja orientalis, possessing various physiological and pharmacological activities. However, currently available thuja orientalis essential oils and thuja extracts vary in composition and efficacy, and most products have not been researched and developed for specific therapeutic effects. Therefore, developing a thuja orientalis essential oil with clearly defined efficacy has significant practical importance and application value. Summary of the Invention

[0004] The purpose of this invention is to provide a cypress essential oil for treating burns and for calming the nerves and aiding sleep, and a method for preparing the same, in order to solve the technical problems mentioned in the background section.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a cypress essential oil for treating burns and promoting calming and sleep, comprising the following raw materials:

[0006] The ingredients are branches and trunks of the Chinese cypress tree, ethanol, and water, in a ratio of 0.2:0.1:1:2.

[0007] A method for preparing *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep, comprising the following preparation steps:

[0008] S1. Raw material preparation: Select branches and trunks of cliff cypress trees, remove impurities and diseased or pest-infested parts, clean them thoroughly and dry them for later use.

[0009] S2. Crushing: Crush the cleaned branches and trunks to obtain cypress powder;

[0010] S3. Soaking: Soak the cypress powder in a solvent;

[0011] S4. Extraction: The soaked cypress powder is extracted with ultrasonic assistance to obtain cypress extract;

[0012] S5. Filtration and Concentration: After ultrasonic extraction, the extract of *Thuja orientalis* is filtered to obtain *Thuja orientalis* solution; the *Thuja orientalis* solution is then concentrated under vacuum to obtain *Thuja orientalis* concentrate.

[0013] S6. Refining: The concentrated liquid of Chinese cypress is refined to obtain Chinese cypress essential oil and Chinese cypress extract.

[0014] S7. Inspection and Packaging: The quality of the *Platycladus orientalis* essential oil extract is inspected, and after passing the inspection, it is packaged to obtain the *Platycladus orientalis* essential oil extract product of this invention.

[0015] Preferably, in step S1, the cleaned branches and trunks of the cliff cypress tree are placed in a well-ventilated, sunny place to dry in order to ensure that the branches and trunks are dry.

[0016] Preferably, in step S2, after the branches and trunks of the Chinese cypress tree are completely dried, a pulverizer is used to completely pulverize the branches and trunks to release more aromatic components and facilitate subsequent preparation operations.

[0017] Preferably, the solvent in S3 is an ethanol-water mixture, with a volume ratio of ethanol to water of 1:2, and the soaking time is 24 hours.

[0018] Preferably, the ultrasonic extraction temperature in step S4 is 40°C and the extraction time is 2 hours. Using ultrasound can accelerate the penetration of the solvent into the cypress cells and break down the cell wall to release active ingredients.

[0019] Preferably, in step S5, filter paper or filter membrane is used to filter the solid waste in the cypress essential oil extract to maintain its quality and purity.

[0020] Preferably, the instrument used for vacuum concentration in S5 is a rotary evaporator or a vacuum evaporator, in order to remove most of the solvent from the cypress essential oil extract.

[0021] Preferably, the purification method in S6 includes steam distillation, supercritical CO2 extraction, and molecular distillation.

[0022] Preferably, the main components and their relative contents of the cypress essential oil extract obtained in S6 are: 41.52% cypressene, 21.16% cedrol, 19.76% α-cedrene, 8.39% cypressene, 4.12% longleaf pinene, and 2.65% β-cedrene.

[0023] Compared with the prior art, the beneficial effects of the present invention are:

[0024] (1) The cypress essential oil and cypress extract in this invention have significant therapeutic effects on burns. When the skin is burned, the cypress essential oil and cypress extract can be applied directly to the affected area 2-3 times a day to effectively relieve pain and promote wound healing.

[0025] (2) The cypress essential oil and cypress extract in this invention have a good antipruritic effect. When itching symptoms are caused by mosquito bites, the cypress essential oil and cypress extract can be applied directly to the itchy area. The itching can be relieved in a few minutes. At the same time, it also has a relieving and inhibitory effect on itching caused by wounds.

[0026] (3) The cypress essential oil and cypress extract in this invention have the effects of calming the mind, promoting sleep and relieving anxiety. For people with symptoms such as insomnia and anxiety, they can use atomization or direct application or add the cypress essential oil and cypress extract to an aromatherapy lamp before going to bed so as to achieve the absorption of the cypress essential oil and cypress extract, which helps to relax the mind and body and improve sleep quality.

[0027] In summary, the *Platycladus orientalis* essential oil extract of the present invention exhibits significant effects in treating burns, relieving itching, and calming the nerves, promoting sleep, and alleviating anxiety. Compared with traditional methods, the *Platycladus orientalis* essential oil extract of the present invention has higher purity and more clearly defined efficacy, and its preparation process is simple and inexpensive, showing broad prospects and application potential. Attached Figure Description

[0028] Figure 1 This is a schematic diagram illustrating the preparation process of the *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep, as described in this invention. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] Please see Figure 1 This invention provides a technical solution: a cypress essential oil extract for treating burns and promoting calming and sleep, comprising the following raw materials:

[0031] The ingredients are branches and trunks of the Chinese cypress tree, ethanol, and water, in a ratio of 0.2:0.1:1:2.

[0032] Example 1

[0033] A method for preparing *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep, comprising the following preparation steps:

[0034] S1. Raw material preparation: Select branches and trunks of cliff cypress trees, remove impurities and diseased or pest-infested parts, clean them thoroughly and dry them for later use.

[0035] S2. Crushing: Crush the cleaned branches and trunks to obtain cypress powder;

[0036] S3. Soaking: Soak the cypress powder in a solvent, specifically an ethanol-water mixture with a volume ratio of ethanol to water of 1:2, for 24 hours.

[0037] S4. Extraction: The soaked cypress powder is subjected to ultrasonic-assisted extraction to obtain cypress extract. The ultrasonic extraction temperature is 40℃ and the extraction time is 2 hours. Ultrasonic waves can accelerate the penetration of the solvent into the cypress cells and destroy the cell wall to release active ingredients.

[0038] S5. Filtration and Concentration: After ultrasonic extraction, the extract of *Thuja orientalis* is filtered to obtain *Thuja orientalis* solution; the *Thuja orientalis* solution is then concentrated under vacuum to obtain *Thuja orientalis* concentrate.

[0039] S6. Refining: The concentrated liquid of *Thuja orientalis* is refined by steam distillation to obtain *Thuja orientalis* essential oil extract.

[0040] S7. Inspection and Packaging: The quality of the *Platycladus orientalis* essential oil extract is inspected, and after passing the inspection, it is packaged to obtain the *Platycladus orientalis* essential oil extract product of this invention.

[0041] In step S1, the cleaned branches and trunks of the cliff cypress tree are placed in a well-ventilated, sunny place to dry in order to ensure that the branches and trunks are dry.

[0042] In step S2, after the branches and trunks of the Chinese cypress tree are completely dried, a pulverizer is used to completely pulverize the branches and trunks to release more aromatic components and facilitate subsequent preparation operations.

[0043] In step S5, filter paper or filter membrane is used to filter the solid waste in the cypress essential oil extract to maintain its quality and purity.

[0044] The instrument used for vacuum concentration in S5 is a rotary evaporator or a vacuum evaporator, in order to remove most of the solvent from the cypress essential oil extract.

[0045] The main components and their relative contents of the *Platycladus orientalis* essential oil extract obtained in S6 are as follows: 41.52% thujone, 21.16% cedrol, 19.76% α-thujone, 8.39% thujone, 4.12% pinene, and 2.65% β-cedrene.

[0046] The equipment is simple, easy to operate, low in cost, and has a large output. This method is suitable for large-scale production and can better preserve the natural aroma and active ingredients of the cypress essential oil extract.

[0047] Example 2

[0048] A method for preparing *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep, comprising the following preparation steps:

[0049] S1. Raw material preparation: Select branches and trunks of cliff cypress trees, remove impurities and diseased or pest-infested parts, clean them thoroughly and dry them for later use.

[0050] S2. Crushing: Crush the cleaned branches and trunks to obtain cypress powder;

[0051] S3. Soaking: Soak the cypress powder in a solvent, specifically an ethanol-water mixture with a volume ratio of ethanol to water of 1:2, for 24 hours.

[0052] S4. Extraction: The soaked cypress powder is subjected to ultrasonic-assisted extraction to obtain cypress extract. The ultrasonic extraction temperature is 40℃ and the extraction time is 2 hours. Ultrasonic waves can accelerate the penetration of the solvent into the cypress cells and destroy the cell wall to release active ingredients.

[0053] S5. Filtration and Concentration: After ultrasonic extraction, the extract of *Thuja orientalis* is filtered to obtain *Thuja orientalis* solution; the *Thuja orientalis* solution is then concentrated under vacuum to obtain *Thuja orientalis* concentrate.

[0054] S6. Refining: The concentrated liquid of Chinese cypress is refined by supercritical CO2 extraction to obtain Chinese cypress essential oil and Chinese cypress extract.

[0055] S7. Inspection and Packaging: The quality of the *Platycladus orientalis* essential oil extract is inspected, and after passing the inspection, it is packaged to obtain the *Platycladus orientalis* essential oil extract product of this invention.

[0056] In step S1, the cleaned branches and trunks of the cliff cypress tree are placed in a well-ventilated, sunny place to dry in order to ensure that the branches and trunks are dry.

[0057] In step S2, after the branches and trunks of the Chinese cypress tree are completely dried, a pulverizer is used to completely pulverize the branches and trunks to release more aromatic components and facilitate subsequent preparation operations.

[0058] In step S5, filter paper or filter membrane is used to filter the solid waste in the cypress essential oil extract to maintain its quality and purity.

[0059] The instrument used for vacuum concentration in S5 is a rotary evaporator or a vacuum evaporator, in order to remove most of the solvent from the cypress essential oil extract.

[0060] The main components and their relative contents of the *Platycladus orientalis* essential oil extract obtained in S6 are as follows: 41.52% thujone, 21.16% cedrol, 19.76% α-thujone, 8.39% thujone, 4.12% pinene, and 2.65% β-cedrene.

[0061] Supercritical CO2 extraction can better preserve the activity and aroma of the original substances. In addition, it has the advantages of good selectivity, high extraction efficiency and mild operating conditions.

[0062] Example 3

[0063] A method for preparing *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep, comprising the following preparation steps:

[0064] S1. Raw material preparation: Select branches and trunks of cliff cypress trees, remove impurities and diseased or pest-infested parts, clean them thoroughly and dry them for later use.

[0065] S2. Crushing: Crush the cleaned branches and trunks to obtain cypress powder;

[0066] S3. Soaking: Soak the cypress powder in a solvent, specifically an ethanol-water mixture with a volume ratio of ethanol to water of 1:2, for 24 hours.

[0067] S4. Extraction: The soaked cypress powder is subjected to ultrasonic-assisted extraction to obtain cypress extract. The ultrasonic extraction temperature is 40℃ and the extraction time is 2 hours. Ultrasonic waves can accelerate the penetration of the solvent into the cypress cells and destroy the cell wall to release active ingredients.

[0068] S5. Filtration and Concentration: After ultrasonic extraction, the extract of *Thuja orientalis* is filtered to obtain *Thuja orientalis* solution; the *Thuja orientalis* solution is then concentrated under vacuum to obtain *Thuja orientalis* concentrate.

[0069] S6. Refining: The concentrated liquid of *Thuja orientalis* is refined by molecular distillation to obtain *Thuja orientalis* essential oil extract.

[0070] S7. Inspection and Packaging: The quality of the *Platycladus orientalis* essential oil extract is inspected, and after passing the inspection, it is packaged to obtain the *Platycladus orientalis* essential oil extract product of this invention.

[0071] In step S1, the cleaned branches and trunks of the cliff cypress tree are placed in a well-ventilated, sunny place to dry in order to ensure that the branches and trunks are dry.

[0072] In step S2, after the branches and trunks of the Chinese cypress tree are completely dried, a pulverizer is used to completely pulverize the branches and trunks to release more aromatic components and facilitate subsequent preparation operations.

[0073] In step S5, filter paper or filter membrane is used to filter the solid waste in the cypress essential oil extract to maintain its quality and purity.

[0074] The instrument used for vacuum concentration in S5 is a rotary evaporator or a vacuum evaporator, in order to remove most of the solvent from the cypress essential oil extract.

[0075] The main components and their relative contents of the *Platycladus orientalis* essential oil extract obtained in S6 are as follows: 41.52% thujone, 21.16% cedrol, 19.76% α-thujone, 8.39% thujone, 4.12% pinene, and 2.65% β-cedrene.

[0076] By employing molecular distillation, pure aroma components can be easily extracted and impurities in the raw materials can be removed, resulting in a purer cypress essential oil extract. In addition, because the temperature of molecular distillation is relatively low, active ingredients can be preserved, giving the cypress essential oil extract better efficacy.

[0077] Table 1 Results of activity analysis of major compounds

[0078]

[0079]

[0080] Working principle: In preparing this *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep, branches and trunks of *Platycladus orientalis* trees are selected, impurities and diseased parts are removed, and the branches and trunks are cleaned and dried for later use. The cleaned branches and trunks are crushed into *Platycladus orientalis* powder, which is then soaked in a solvent. The soaked *Platycladus orientalis* powder is then subjected to ultrasonic-assisted extraction to obtain a *Platycladus orientalis* extract. After ultrasonic extraction, the *Platycladus orientalis* extract is filtered to obtain a *Platycladus orientalis* solution. The *Platycladus orientalis* solution is then vacuum concentrated to obtain a *Platycladus orientalis* concentrate. The *Platycladus orientalis* concentrate is then refined to obtain the *Platycladus orientalis* essential oil extract. The *Platycladus orientalis* essential oil extract is then subjected to quality inspection. If it passes the quality inspection, it is packaged to obtain the *Platycladus orientalis* essential oil extract product of this invention. Content not described in detail in this description belongs to the prior art known to those skilled in the art.

[0081] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A type of cypress essential oil and cypress extract for treating burns and promoting restful sleep, characterized in that, Including the following raw materials: The ingredients are branches and trunks of the Chinese cypress tree, ethanol, and water, in a ratio of 0.2:0.1:1:

2.

2. A method for preparing *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep, characterized in that... The preparation steps include the following: S1. Raw material preparation: Select branches and trunks of cliff cypress trees, remove impurities and diseased or pest-infested parts, clean them thoroughly and dry them for later use. S2. Crushing: Crush the cleaned branches and trunks to obtain cypress powder; S3. Soaking: Soak the cypress powder in a solvent; S4. Extraction: The soaked cypress powder is extracted with ultrasonic assistance to obtain cypress extract; S5. Filtration and Concentration: After ultrasonic extraction, the extract of *Thuja orientalis* is filtered to obtain *Thuja orientalis* solution; the *Thuja orientalis* solution is then concentrated under vacuum to obtain *Thuja orientalis* concentrate. S6. Refining: The concentrated liquid of Chinese cypress is refined to obtain Chinese cypress essential oil and Chinese cypress extract. S7. Inspection and Packaging: The quality of the *Platycladus orientalis* essential oil extract is inspected, and after passing the inspection, it is packaged to obtain the *Platycladus orientalis* essential oil extract product of this invention.

3. The method for preparing the *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 2, characterized in that: In step S1, the cleaned branches and trunks of the cliff cypress tree are placed in a well-ventilated, sunny place to dry in order to ensure that the branches and trunks are dry.

4. The method for preparing the *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 2, characterized in that: In step S2, after the branches and trunks of the Chinese cypress tree are completely dried, a pulverizer is used to completely pulverize the branches and trunks to release more aromatic components and facilitate subsequent preparation operations.

5. The method for preparing the *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 2, characterized in that: The solvent used in S3 is an ethanol-water mixture with a volume ratio of ethanol to water of 1:2, and the soaking time is 24 hours.

6. The method for preparing the *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 2, characterized in that: In S4, the ultrasonic extraction temperature is 40℃ and the extraction time is 2 hours. Using ultrasound can accelerate the penetration of solvent into the cypress cells and break down the cell wall to release active ingredients.

7. The method for preparing the *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 2, characterized in that: In step S5, filter paper or filter membrane is used to filter the solid waste in the cypress essential oil extract to maintain its quality and purity.

8. The method for preparing the *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 7, characterized in that: The instrument used for vacuum concentration in S5 is a rotary evaporator or a vacuum evaporator, in order to remove most of the solvent from the cypress essential oil extract.

9. A method for preparing a *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 2, characterized in that: The purification methods in S6 include steam distillation, supercritical CO2 extraction, and molecular distillation.

10. A method for preparing a *Platycladus orientalis* essential oil extract for treating burns and promoting calming and sleep according to claim 9, characterized in that: The main components and their relative contents of the *Platycladus orientalis* essential oil extract obtained in S6 are as follows: 41.52% thujone, 21.16% cedrol, 19.76% α-thujone, 8.39% thujone, 4.12% pinene, and 2.65% β-cedrene.

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