A composition for regulating scalp microflora and use thereof

Abstract

The application discloses a composition for regulating scalp microecology, which comprises A component and B component, wherein the A component comprises, in parts by weight, 5-15 parts of Phellodendron amurense bark extract, 3-14 parts of Rhizoma Coptidis extract, 5-15 parts of Sophora flavescens root extract, 2-8 parts of Rhus galla chinensis insect gall extract, 2-18 parts of Cnidium monnieri fruit extract, 3-10 parts of Zanthoxylum bungeanum pericarp extract, 1-5 parts of Cinnamomum camphora root extract, 3-12 parts of Zingiber officinale root extract, 5-15 parts of Polygonum multiflorum root extract, 5-25 parts of Japanese Sabina extract and 10-30 parts of Biota orientalis leaf extract; the B component comprises, in parts by weight, 70-90 parts of cocamidopropyl-PG-dimethyl ammonium chloride phosphate ester sodium, 10-30 parts of sodium isostearoyl lactylate and 1-10 parts of dichlorobenzyl alcohol; and the weight ratio of the A component to the B component is 10:1. The composition can effectively regulate the balance of scalp microecology, significantly inhibit the excessive growth and reproduction of common harmful bacteria on the scalp, protect or moderately promote the growth of beneficial bacteria on the scalp, maintain the healthy bacterial community structure and diversity, fundamentally improve the scalp health by restoring and maintaining the balance of the scalp microecology, and has multiple good effects of oil control, hair loss prevention, itching prevention, dandruff removal and the like.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition for regulating scalp microecology and its application. Background Technology

[0002] As an important component of human skin, the scalp's microecological balance is crucial for scalp health and hair growth. A healthy scalp microecology consists of various microorganisms (including bacteria, fungi, and viruses), which interact to maintain the scalp's physiological functions. However, modern fast-paced lifestyles, environmental pollution, improper washing and care habits, and individual differences can easily lead to an imbalance in the scalp microecology, resulting in a series of scalp health problems such as dandruff, itching, excessive sebum secretion, inflammation, and even accelerated hair loss.

[0003] Currently, mainstream anti-hair loss and anti-dandruff shampoos on the market primarily rely on chemical antibacterial agents (such as zinc pyrithione, ketoconazole, and selenium disulfide) to control scalp fungi and bacteria when addressing the aforementioned scalp problems. However, these chemical ingredients have significant limitations: they are often irritating and may cause scalp sensitivity, dryness, and other discomforts; long-term or improper use may also lead to drug resistance in microorganisms, reducing the product's effectiveness; more importantly, they typically exhibit non-selective killing effects on the scalp microbiome, potentially disrupting the normal microecological balance of the scalp, thereby damaging the scalp barrier and even triggering new scalp problems, creating a potential ecological burden. This contradicts the growing consumer demand for gentle, safe, and sustainable skincare products. On the other hand, as consumers increasingly prefer natural and green products, some technologies are exploring the use of herbal extracts as natural functional factors in shampoos and conditioners. Although some natural ingredients claim to have "microecological regulation" effects, the evaluation and verification are still based on the antibacterial effects of Malassezia and Staphylococcus. There is a lack of systematic high-throughput microbial community structure analysis data to support them, making it impossible to achieve functional verification and the positioning of iconic functional ingredients, and it is also difficult to form a technological barrier.

[0004] While there are numerous inventions related to scalp care and hair growth in the existing technology, some deficiencies or limitations remain in achieving comprehensive, safe, and efficient regulation of the scalp microecology. Chinese patent applications CN202211731975A, CN202411056731A, CN202411657442A, and CN202510252902A primarily focus on anti-hair loss efficacy. Their compositions mainly contain ingredients such as ginger, arborvitae, he shou wu, and ginseng, but they do not fully reveal or verify their mechanisms of action and synergistic effects in comprehensively balancing and regulating the scalp microecology. Their efficacy evaluations mostly revolve around indicators such as hair density and hair loss count, lacking in-depth analysis of key microecological indicators such as scalp flora structure and inflammatory factors. The herbal oil-controlling essence disclosed in CN202510091507A contains extracts of various plants such as Phellodendron bark, Coptis root, and Sophora root, and its purpose is solely to control oil. CN202410246326A and CN202411606093 disclose a "hair loss prevention composition that regulates scalp microecology". Its mechanism of action is mainly to inhibit two harmful microorganisms, Malassezia and Staphylococcus aureus, through fermentation products. It is a broader skin care matrix technology solution, but it does not clearly target the comprehensive balance regulation of scalp microecology, nor does it mention the synergistic effect of these ingredients on different bacterial groups in this specific combination.

[0005] Therefore, there is an urgent need for a plant-based compound composition that, through systematic verification of its ability to finely regulate the scalp's microecology using high-throughput data, can fundamentally improve problems caused by microecological imbalances such as dandruff, itching, and excessive sebum secretion, ultimately achieving a triple synergistic effect of relieving itching, preventing hair loss, and removing dandruff. This would fill the technological gap in the current natural plant-based hair care products where the process-efficacy-verification closed loop is insufficient. Summary of the Invention

[0006] The purpose of this invention is to address the above-mentioned technical problems by providing a composition that can effectively regulate the scalp microecology and significantly improve the scalp flora structure, thereby improving excessive scalp oil production, dandruff, scalp itching, folliculitis, or hair loss caused by scalp microecological imbalance.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0008] In a first aspect, the present invention provides a composition for regulating scalp microecology, comprising component A and component B, wherein component A comprises, by weight parts, 5-15 parts of Phellodendron bark extract, 3-14 parts of Coptis root extract, 5-15 parts of Sophora root extract, 2-8 parts of Rhus chinensis gall extract, 2-18 parts of Cnidium monnieri extract, 3-10 parts of Zanthoxylum bungeanum pericarp extract, 1-5 parts of Cinnamomum camphora root extract, 3-12 parts of Zingiber officinale root extract, 5-15 parts of Polygonum multiflorum root extract, 5-25 parts of Swertia japonica extract, and 10-30 parts of Platycladus orientalis leaf extract.

[0009] Component B, by weight, comprises 70-90 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 10-30 parts of sodium isostearyl lactylate, and 1-10 parts of dichlorobenzyl alcohol.

[0010] The weight ratio of component A to component B is 10:1.

[0011] Preferably, component A comprises, by weight, 8-12 parts of Phellodendron bark extract, 5-10 parts of Coptis root extract, 8-12 parts of Sophora root extract, 3-6 parts of Rhus chinensis gall extract, 5-16 parts of Cnidium monnieri extract, 5-8 parts of Zanthoxylum bungeanum pericarp extract, 2-4 parts of Cinnamomum camphora root extract, 5-8 parts of Zingiber officinale root extract, 8-12 parts of Polygonum multiflorum root extract, 7-18 parts of Swertia japonica extract, and 16-24 parts of Platycladus orientalis leaf extract.

[0012] Preferably, component A comprises, by weight, 8-12 parts of Phellodendron bark extract, 5-10 parts of Coptis root extract, 8-10 parts of Sophora root extract, 3-5 parts of Rhus chinensis gall extract, 5-10 parts of Cnidium monnieri extract, 5-7 parts of Zanthoxylum bungeanum pericarp extract, 2-4 parts of Cinnamomum camphora root extract, 5-8 parts of Zingiber officinale root extract, 8-12 parts of Polygonum multiflorum root extract, 7-16 parts of Swertia japonica extract, and 16-20 parts of Platycladus orientalis leaf extract.

[0013] More preferably, component A comprises, by weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Camphor root extract, 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract.

[0014] More preferably, the extracts contain berberine (≥3000 ppm) in the bark extract of Phellodendron chinense, berberine (≥1000 ppm) in the root extract of Coptis chinensis, matrine (≥3000 ppm) in the root extract of Sophora flavescens, gallic acid (≥3000 ppm) in the gall extract of Rhus chinensis, osthol (≥3000 ppm) in the extract of Cnidium monnieri, naringin (≥2000 ppm) in the pericarp extract of Zanthoxylum bungeanum, camphor (≥1000 ppm) in the root extract of Cinnamomum camphora, gingerol (≥3000 ppm) in the root extract of Zingiber officinale, emodin (≥1000 ppm) in the root extract of Polygonum multiflorum, swertiamarin (≥2000 ppm) in the extract of Swertia japonica, and quercetin (≥1000 ppm) in the leaf extract of Platycladus orientalis.

[0015] Preferably, component B comprises, by weight, 75-85 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 12-20 parts of sodium isostearyl lactylate, and 3-7 parts of dichlorobenzyl alcohol.

[0016] Preferably, component B comprises, by weight, 75-80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15-20 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol.

[0017] More preferably, component B comprises, by weight, 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol.

[0018] Secondly, the present invention provides the use of the composition in the preparation of cosmetics for regulating the scalp microecological balance.

[0019] Thirdly, the present invention provides the use of the composition in the preparation of cosmetics for oil control, dandruff removal, relieving scalp itching, and preventing hair loss.

[0020] Fourthly, the present invention provides a shampoo and conditioner containing the composition for regulating scalp microecology described in the present invention. Preferably, the composition is present in the shampoo and conditioner at a weight percentage of 1-5%, more preferably 2-4%, and most preferably 2.5%.

[0021] The present invention has the following beneficial effects:

[0022] (1) Effectively regulates the scalp microecological balance: It can significantly inhibit the excessive growth and reproduction of common harmful bacteria on the scalp (such as Malassezia furfur, Staphylococcus aureus, Propionibacterium acnes, etc.). It can protect or moderately promote the growth of beneficial bacteria on the scalp, maintaining a healthy flora structure and diversity. By restoring and maintaining the balance of the scalp microecology, it fundamentally improves scalp health.

[0023] (2) Multi-effect improvement of scalp problems and comprehensive enhancement of scalp health: Powerful oil control and improvement of greasiness. By regulating the microorganisms related to sebum secretion and acting directly, it effectively reduces excessive scalp oil secretion, keeping the scalp fresh for a long time; Significant dandruff removal and relief of scalp itching. It effectively inhibits the microorganisms that cause dandruff and scalp itching (especially Malassezia furfur), reduces dandruff production, and quickly relieves scalp itching and discomfort; Strengthens hair roots and assists in preventing hair loss. By creating a healthy scalp microecological environment, it improves the nutritional supply to hair follicles, enhances hair follicle activity, and thus strengthens hair roots, helping to reduce hair loss caused by microecological imbalance, inflammation, or oil blockage; Soothes inflammation and repairs the barrier. The composition contains The multiple ingredients possess natural anti-inflammatory and soothing properties, which can reduce scalp inflammation. Simultaneously, a healthy scalp microecology helps repair and enhance the scalp's skin barrier function. Natural and gentle, it is safe to use. All components are natural plant extracts, which generally have better biocompatibility and lower irritation compared to chemically synthesized ingredients, making them suitable for long-term and daily care. Synergistic effects and comprehensive efficacy: the 11 plant extracts each possess unique bioactivity. Through the specific combination of these ingredients, a synergistic effect is achieved, resulting in a composition that demonstrates superior overall effects in regulating the scalp microecology and improving scalp problems compared to single ingredients or simple mixtures.

[0024] (3) Optimization design for scalp microecology: The 11 plant extracts in the formula have clear microecological regulation effects, such as the antibacterial synergy of Phellodendron bark and Sophora flavescens, the promotion of microcirculation by ginger and Polygonum multiflorum, and the regulation of oil balance by Platycladus orientalis leaf. Unlike the traditional empirical "drug pair", the selection of materials is guided by the microecological mechanism, focusing on regulating the fungal-bacterial balance, improving diversity, and restoring the function of resident flora. This provides a new direction for the design of skin microecological intervention-type shampoo and care products.

[0025] (4) Efficacy verification by integrating high-throughput sequencing data: This invention uses a dual-group design of experimental and control groups and applies high-throughput sequencing on both 16S and ITS platforms to scientifically verify the efficacy of microecological regulation; the data analysis covers multi-dimensional indicators such as α diversity, β difference, abundance change, and synergistic relationship of the microbiome, and shows the triple efficacy of "anti-hair loss, anti-itch and anti-dandruff" from the complete microecology. Attached Figure Description

[0026] Figure 1 The changes in scalp microecology before and after trial use in volunteers are shown. Detailed Implementation

[0027] To facilitate understanding of the present invention, a more complete description will be given below with reference to specific embodiments. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention.

[0028] In the description of this invention, unless otherwise explicitly defined, terms such as heating, cleaning, weighing, and freezing should be interpreted broadly. Those skilled in the art can reasonably determine the specific meaning of the above terms in this invention in conjunction with the specific content of the technical solution.

[0029] In the description of this invention, references to terms such as "some embodiments" and "examples" indicate that the specific methods or materials described in connection with that embodiment or example are included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the specific methods and materials described may be combined in any suitable manner in one or more embodiments or examples.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

[0031] Unless otherwise specified, the experimental methods used in the following examples and comparative examples are conventional methods, and the materials and reagents used are commercially available unless otherwise specified.

[0032] The plant extract composition for regulating scalp microecology proposed in this invention is characterized by its unique and synergistic compound system of 11 plant extracts. It aims to achieve refined regulation of scalp microecology through the synergistic effect of multiple targets and pathways, thereby effectively improving a series of scalp health problems such as dandruff, itchy scalp, excessive sebum secretion, inflammation and hair loss.

[0033] The preparation of the composition of this invention aims to employ optimized extraction processes tailored to the characteristics of each plant to maximize the retention and enrichment of its key active ingredients, ensuring the mildness, safety, and solvent-free nature of the extract. Quantitative detection of the target components is performed using high-performance liquid chromatography (HPLC), ultimately resulting in a highly active, high-quality compound extract. All plant materials were ordered from the Bozhou Traditional Chinese Medicine Industrial Park.

[0034] The bark of the Phellodendron amurense tree is from the Rutaceae family. Phellodendron amurense The dried bark of Rupr.

[0035] Coptis root, also known as Huanglian (Coptis chinensis), is a plant belonging to the Ranunculaceae family. Chinese Coptis Franch., Triangular Leaf Coptis Copts deltoid CYCheng et Hsiao or Yunlian Coptic tea The dried rhizome of Wall.

[0036] Sophora flavescens root, the root of the legume Sophora flavescens. Sophora flavense The dried root of Ait.

[0037] Gallnuts of the sumac tree, also known as five-fold gallnuts, are a type of gallnut from the sumac plant (Rhus chinensis) of the Anacardiaceae family. Chinese rose Mill., Green Bran Poplar Rhus potaninii Maxim. or red gluten poplar Rhus punjabensis Galls on leaves of Stew. var. sinica (Diels) Rehd. et Wils., mainly caused by gall aphids. Chinese Melaphis (Bell) Baker is a parasitic organism. Harvested in autumn, it is briefly boiled or steamed in boiling water until the surface turns gray to kill the aphids, then removed and dried.

[0038] Cnidium monnieri, a plant belonging to the Apiaceae family. Cnidium monnieri The dried, ripe fruit of (L.) Cuss.

[0039] The pericarp of Sichuan pepper is the fruit peel of the green pepper plant (Rutaceae family). Zanthoxylum schinifolium Sieb.et Zucc. or Sichuan pepper Zanthoxylum bungeanum The dried, mature pericarp of Maxim.

[0040] Camphor tree root, belonging to the Lauraceae family of camphor trees. Cinnamon camphor The dried root of (L.) Presl.

[0041] Ginger root, the root of the ginger plant (Zingiber officinale). Ginger officinalis The dried rhizome of Rosc.

[0042] The root of Polygonum multiflorum is the root of the Polygonum multiflorum plant (Polygonaceae family). Polygonum multiflorum Dried tuberous roots of Thunb.

[0043] Japanese swert, a plant belonging to the Gentianaceae family. Swertia diluta The dried whole herb of (Turcz.) Benth. etHook. f. var. tosaensis (Makino) Hara.

[0044] Oriental arborvitae leaves, from the cypress family plant Oriental arborvitae. Oriental platycladus Dry shoots and leaves of (L.) Franco.

[0045] The preparation steps for each plant extract component are as follows:

[0046] 1. Preparation of plant powder

[0047] Weigh the raw medicinal materials according to the required dosage, dry and grind them into a fine powder of 40-80 mesh, sieve, and set aside.

[0048] 2. Stepwise extraction: Select the most suitable extraction solvent and extraction method based on the physicochemical properties of the main active ingredients of each medicinal material.

[0049] 2.1 Extraction of water-soluble components

[0050] (1) Extracted plants: Rhus chinensis gall and Swertia japonica.

[0051] (2) Solvent: water.

[0052] (3) Material-liquid ratio: 1:10 (g / mL) (selectable range 1:8-1:15 (g / mL)).

[0053] (4) Extraction method: Ultrasonic-assisted water extraction. After mixing the medicinal materials with water, place them in an ultrasonic extraction device for ultrasonic extraction. The ultrasonic frequency is 40kHz (selectable range 28-40kHz), and the ultrasonic power is 400W (selectable range 300-500W). The extraction temperature is 50℃ (selectable range 40-60℃).

[0054] (3) Extraction time and number of times: Each extraction lasts 1.5-2.5 hours (2 hours is the best option), and a total of 2 extractions are performed to obtain an aqueous solution, which is the plant extract.

[0055] 2.2 Extraction of alcohol-soluble / semi-volatile components

[0056] (1) Extracted plants: bark of Phellodendron bark, root of Coptis chinensis, root of Sophora flavescens, Cnidium monnieri, pericarp of Zanthoxylum bungeanum, leaves of Platycladus orientalis, root of ginger, root of Polygonum multiflorum.

[0057] (2) Solvent: 50% v / v ethanol solution (30%-70% (v / v) ethanol solution can be selected).

[0058] (3) Material-liquid ratio: 1:8 (g / mL) (selectable range 1:5-1:10 (g / mL)).

[0059] (4) Extraction method: low-temperature maceration / percolation, supplemented by ultrasound. Mix the medicinal material with ethanol solution and macerate at room temperature (20°C) for 48 hours. During this period, perform ultrasound treatment once a day (30 minutes each time, 40kHz, ultrasound power 400W) to improve extraction efficiency.

[0060] (5) Subsequent processing: The extract needs to be concentrated under reduced pressure to remove ethanol residue and obtain an aqueous solution, which is the plant extract.

[0061] 2.3 Extraction of volatile oil components

[0062] (1) Extracted plant: camphor tree root.

[0063] (2) Solvent: water.

[0064] (3) Extraction method: steam distillation.

[0065] (4) Process parameters: Place the medicinal material and water in a distillation kettle at a material-to-liquid ratio of 1:8 (g / mL) (selectable range 1:5-1:10 (g / mL)), heat with steam, distill for 2.5 hours, collect the condensate and let it stand to separate the oil. The upper volatile oil is the camphor root extract.

[0066] 3. Extract Quality Control Plan (Indicator Components)

[0067] To ensure the stable expression of technological advantages, an HPLC analysis method for the main chemical substances in plants was developed:

[0068] (1) Chromatographic column: C18 reversed phase column (e.g., Agilent ZORBAX Eclipse Plus C18 4.6×250mm, 5μm).

[0069] (2) Mobile phase: Different mobile phases are used for detection according to the detection methods provided in the Chinese Pharmacopoeia.

[0070] (3) Flow rate: 1.0 mL / min.

[0071] (4) Detection wavelength: Selected according to the maximum absorption of each component: berberine 225 nm; berberine 345 nm; matrine 220 nm; gallic acid 272 nm; osthol 322 nm; naringin 280 nm; camphor 289 nm; gingerol 280 nm; emodin 285 nm; swertiamarin 238 nm; quercetin 360 nm.

[0072] (5) Column temperature: 30℃.

[0073] (6) Injection volume: 10 μL.

[0074] The compound plant composition of the present invention comprises the following components:

[0075]

[0076] Example 1

[0077] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to form a brownish-yellow compound plant composition 1.

[0078] Example 2

[0079] By weight, 12 parts of Phellodendron bark extract, 10 parts of Coptis root extract, 8 parts of Sophora root extract, 5 parts of Rhus chinensis gall extract, 8 parts of Cnidium monnieri extract, 5 parts of Zanthoxylum bungeanum pericarp extract, 4 parts of Cinnamomum camphora root extract, 8 parts of Zingiber officinale root extract, 12 parts of Polygonum multiflorum root extract, 16 parts of Swertia japonica extract, and 18 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to form a brownish-yellow compound plant composition 2.

[0080] Example 3

[0081] By weight, 8 parts of Phellodendron bark extract, 5 parts of Coptis root extract, 8 parts of Sophora root extract, 3 parts of Rhus chinensis gall extract, 5 parts of Cnidium monnieri extract, 5 parts of Zanthoxylum bungeanum pericarp extract, 2 parts of Camphor root extract, 5 parts of Zingiber officinale root extract, 8 parts of Polygonum multiflorum root extract, 7 parts of Swertia japonica extract, and 16 parts of Platycladus orientalis leaf extract were taken as component A; 75 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 20 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to form a brownish-yellow compound plant composition 3.

[0082] Example 4

[0083] By weight, 12 parts of Phellodendron bark extract, 10 parts of Coptis root extract, 12 parts of Sophora flavescens root extract, 6 parts of Rhus chinensis gall extract, 16 parts of Cnidium monnieri extract, 8 parts of Zanthoxylum bungeanum pericarp extract, 4 parts of Cinnamomum camphora root extract, 8 parts of Zingiber officinale root extract, 12 parts of Polygonum multiflorum root extract, 18 parts of Swertia japonica extract, and 24 parts of Platycladus orientalis leaf extract were taken as component A; 85 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 12 parts of sodium isostearyl lactylate, and 3 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to form a brownish-yellow compound plant composition 4.

[0084] Example 5

[0085] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 75 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 18 parts of sodium isostearyl lactylate, and 7 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to form a brownish-yellow compound plant composition 5.

[0086] Comparative Example 1

[0087] Comparative Example 1 of the present invention was prepared by taking 10 parts by weight of Phellodendron bark extract, 8 parts by Coptis root extract, 10 parts by Sophora root extract, 4 parts by Rhus chinensis gall extract, 10 parts by weight of Cnidium monnieri extract, 7 parts by weight of Zanthoxylum bungeanum fruit peel extract, 3 parts by weight of camphor root extract, 6 parts by weight of ginger root extract, 10 parts by weight of Polygonum multiflorum root extract, 13 parts by weight of Swertia japonica extract, and 20 parts by weight of Platycladus orientalis leaf extract.

[0088] Comparative Example 2

[0089] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 50 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 45 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 2 of the present invention.

[0090] Comparative Example 3

[0091] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 50 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, 5 parts of dichlorobenzyl alcohol, and 30 parts of purified water were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 3 of the present invention.

[0092] Comparative Example 4

[0093] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of purified water were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 4 of the present invention.

[0094] Comparative Example 5

[0095] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of purified water, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 5 of the present invention.

[0096] Comparative Example 6

[0097] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of purified water, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 6 of the present invention.

[0098] Comparative Example 7

[0099] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of purified water, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 7 of the present invention.

[0100] Comparative Example 8

[0101] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of purified water, 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 8 of the present invention.

[0102] Comparative Example 9

[0103] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of purified water, 3 parts of Camphor tree root extract, 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 9 of the present invention.

[0104] Comparative Example 10

[0105] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of purified water, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 10 of the present invention.

[0106] Comparative Example 11

[0107] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of purified water, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 11 of the present invention.

[0108] Comparative Example 12

[0109] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of purified water, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 12 of the present invention.

[0110] Comparative Example 13

[0111] By weight, 10 parts of Phellodendron bark extract, 8 parts of purified water, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 13 of the present invention.

[0112] Comparative Example 14

[0113] By weight, 10 parts of purified water, 8 parts of Coptis chinensis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum fruit peel extract, 3 parts of Cinnamomum camphora root extract, 6 parts of Zingiber officinale root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of cocamidopropyl-PG-dimethylammonium chloride phosphate sodium, 15 parts of isostearyl lactylate sodium, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 14 of the present invention.

[0114] Comparative Example 15

[0115] By weight, 13 parts of yeast / Acetobacter xylinum / black tea fermentation product (Guangzhou Youke Biotechnology Co., Ltd.), 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of cocamidopropyl-PG-dimethylammonium chloride phosphate sodium, 15 parts of isostearyl lactylate sodium, and 5 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 15 of the present invention.

[0116] Comparative Example 16

[0117] By weight, 13 parts of Lactobacillus / soybean extract fermentation product filtrate (Sentinol, France), 13 parts of Galactomyces-like bacteria fermentation product filtrate (Guangzhou Youke Biotechnology Co., Ltd.), 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of cocamidopropyl-PG-dimethylammonium chloride phosphate sodium, 15 parts of isostearyl lactylate sodium, and 5 parts of dichlorobenzyl alcohol were taken as component B. Component A and component B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 16 of the present invention.

[0118] Comparative Example 17

[0119] Comparative Example 17 of the present invention was prepared by taking 80 parts by weight of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts by weight of sodium isostearyl lactylate, and 5 parts by weight of pyridone ethanolamine salt.

[0120] Comparative Example 18

[0121] Comparative Example 18 of the present invention was prepared by taking 80 parts by weight of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts by weight of sodium isostearyl lactylate, and 5 parts by weight of zinc pyrithione.

[0122] Comparative Example 19

[0123] Comparative Example 19 of the present invention was prepared by adding a blank shampoo without any compound plant composition.

[0124] Comparative Example 20

[0125] Comparative Example 20 of the present invention was prepared by taking 80 parts by weight of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts by weight of sodium isostearyl lactylate, and 5 parts by weight of dichlorobenzyl alcohol.

[0126] Comparative Example 21

[0127] By weight, 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of ginseng extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 21 of the present invention.

[0128] The preparation method for ginseng extract is the same as that for Polygonum multiflorum root extract.

[0129] Comparative Example 22

[0130] By weight, 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of sodium cocamidopropyl-PG-dimethylammonium chloride phosphate, 15 parts of sodium isostearyl lactylate, and 5 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 22 of the present invention.

[0131] Comparative Example 23

[0132] By weight, 6 parts of ginger root extract, 10 parts of Polygonum multiflorum root extract, 13 parts of Swertia japonica extract, and 20 parts of Platycladus orientalis leaf extract were taken as component A; 80 parts of cocamidopropyl-PG-dimethylammonium chloride phosphate sodium, 15 parts of isostearyl lactylate sodium, and 5 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 23 of the present invention.

[0133] Comparative Example 24

[0134] Comparative Example 24 of the present invention was prepared by taking 10 parts by weight of Phellodendron bark extract, 8 parts by Coptis root extract, 10 parts by Sophora root extract, 4 parts by Rhus chinensis gall extract, 10 parts by weight of Cnidium monnieri extract, 7 parts by weight of Zanthoxylum bungeanum fruit peel extract, and 3 parts by weight of Camphor root extract.

[0135] Comparative Example 25

[0136] By weight, 10 parts of Phellodendron bark extract, 8 parts of Coptis root extract, 10 parts of Sophora flavescens root extract, 4 parts of Rhus chinensis gall extract, 10 parts of Cnidium monnieri extract, 7 parts of Zanthoxylum bungeanum pericarp extract, and 3 parts of Cinnamomum camphora root extract were taken as component A; 80 parts of cocamidopropyl-PG-dimethylammonium chloride phosphate sodium, 15 parts of isostearyl lactylate sodium, and 5 parts of dichlorobenzyl alcohol were taken as component B. Components A and B were mixed and dispersed evenly at a weight ratio of 10:1 to prepare Comparative Example 25 of the present invention.

[0137] The composition for regulating scalp microecology described in this invention can be added to shampoo and hair care products. Preferably, the weight percentage of the composition in the shampoo and hair care products is 1-5%, more preferably 2-4%, and most preferably 2.5%.

[0138] The following uses a shampoo formula as an example to test the antibacterial and anti-hair loss effects of products containing the composition of the present invention, but the actual application of the composition of the present invention is not limited to this.

[0139] Table 1. Shampoo formulas with antibacterial and anti-hair loss effects

[0140]

[0141] Performance testing

[0142] Experiment 1: Stability Experiment

[0143] The efficacy shampoo samples containing the compositions of Examples 1-5 or Comparative Examples 1-25 and their corresponding aqueous solutions were stored at 4°C (low temperature), 25°C (room temperature), and 55°C (high temperature) for a certain period of time (1 month). The presence of phenomena such as layering, precipitation, water separation, crystallization, discoloration, turbidity, and floating oil in Examples 1-5 and Comparative Examples 1-25 was observed and recorded.

[0144] Results analysis:

[0145] Examples 1–5: Under conditions of 4℃ / 25℃ / 55℃, after one month of observation, the system remained "transparent / pale yellow and stable," with no stratification, oil separation, significant discoloration, or precipitation observed. Comparative analysis with the comparative examples: Comparative Example 1 showed oily stratification in the aqueous phase; Comparative Examples 2, 3, and 24 exhibited unstable characteristics such as whitishness / deepening of color / stratification. Furthermore, a comparison between Comparative Example 24 and Comparative Example 25 revealed the same pattern. Sodium cocamidopropyl PG-dimethylammonium chloride phosphate provides hydrophilic-lipophilic interface stability, while sodium isostearyl lactylate aids emulsification and film formation, forming the basis of system stability. The ratio of these two types of additives in the examples is reasonable, ensuring the maintenance of the emulsion phase structure at 4–55℃. Dichlorobenzyl alcohol provides antimicrobial function, and together with antioxidant / phenolic components in plant extracts (such as ginger root and camphor root), it reduces the risk of turbidity and oxidative discoloration caused by microorganisms. When the emulsifier / surfactant was removed or significantly reduced (e.g., Comparative Examples 1, 2, 3), the system exhibited stratification / whitening. Removing certain plants individually (e.g., Comparative Examples 4–14) had no significant effect, indicating that component B has a significant effect on stability.

[0146] This demonstrates that the surface-active / emulsifying system, dichlorobenzyl alcohol, and plant-based antioxidants in component B of the composition collectively ensured the significant stability of Examples 1–5; the absence of any of these components (see Comparative Example 1) resulted in stratification / emulsification failure.

[0147] Table 2. Stability Test Results

[0148]

[0149] Experiment 2. Antibacterial Performance Test

[0150] The antibacterial performance testing scheme is based on the suspension method in the industry standard QB / T 2738-2023 (Evaluation Method for Antibacterial and Antimicrobial Effects of Daily Chemical Products).

[0151] The following standard strains were used in the experiment:

[0152] Bacteria: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 12228.

[0153] Fungus: Candida albicans ATCC 10231.

[0154] Anaerobic bacteria and resident bacteria of the skin: Propionibacterium acnes (ATCC 6919), Malassezia furfur (ATCC 14521).

[0155] Sample concentration test: The sample to be tested was diluted 1:1 with standard hard water and mixed thoroughly. The antibacterial performance of the sample is shown in Table 3 below.

[0156] Results analysis:

[0157] The compositions of the present invention shown in Examples 1-5 exhibited extremely high broad-spectrum antibacterial activity against six test bacterial species (Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Candida albicans, Malassezia furfur, and Propionibacterium acnes), with an overall inhibition rate of ≥99.8%. In contrast, the antibacterial activity of Comparative Example 12 was significantly reduced, with a substantial decrease in the average inhibition rate, especially against Propionibacterium acnes, where the inhibition rate was only 20.1%. This result indicates that in the absence of Sophora flavescens, the composition's inhibitory ability against Propionibacterium acnes in the oily environment is weakened, which may lead to the dominance of this bacterial group and thus disrupt the skin's microecological balance. Comparative Example 14 also showed a low inhibition rate, indicating that Phellodendron amurense is one of the key components in maintaining the overall bacterial control ability of the composition. Comparative Example 19 (blank control) showed no antibacterial activity. When Comparative Example 17 and Comparative Example 18 used OCT (pyrrolidone ethanolamine salt) and ZPT (zinc pyrithione) as preservatives / fungicides, their antibacterial spectrum showed a significant imbalance: they exhibited strong inhibitory effects on some bacterial species, while showing weak inhibition on others. This indicates that a single chemical fungicide cannot replicate the broad-spectrum antibacterial properties of the plant-based compound composition of this invention.

[0158] Analysis of experimental data from Examples 1 and Comparative Examples 23-25 ​​shows that the compound plant composition of the present invention (Example 1) exhibits an inhibition rate of up to 99.99% against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Candida albicans, Malassezia furfur, and Propionibacterium acnes, demonstrating highly efficient and broad-spectrum antibacterial activity. In contrast, the antibacterial performance of Comparative Example 23 (significantly reducing the number of plant species in component A) is significantly reduced across the board, especially against Staphylococcus aureus and Propionibacterium acnes, where the inhibition rates drop to 67.9% and 68.3%, respectively. This confirms that the omitted plant components such as Phellodendron amurense, Coptis chinensis, and Sophora flavescens are key sources of efficacy against specific bacterial species. A comparison between Comparative Example 24 (containing only component A) and Comparative Example 25 (component A incomplete but containing complete component B) further demonstrates that component B can significantly enhance the antibacterial efficacy of the basic plant formulation (e.g., Comparative Example 25 shows an inhibition rate of over 90% against most bacterial species), and this depends on the integrity of component A. Most importantly, the inhibition rate of Propionibacterium acnes in Comparative Example 25 (81.8%) was significantly lower than that in Example 1 (99.99%). This key difference demonstrates that there is a synergistic effect between the complete combination of plant components (component A) and the chemical auxiliary components (component B). Both are indispensable and together achieve a comprehensive and balanced high-efficiency broad-spectrum antibacterial effect that cannot be achieved by a single component.

[0159] Scalp health does not depend on the complete eradication of a single bacterial species, but rather on effectively inhibiting pathogenic bacteria (such as Malassezia, Propionibacterium acnes, and Staphylococcus aureus) while minimizing the impact on beneficial or neutral flora. This invention achieves a "broad-spectrum and balanced" antibacterial mode through the synergistic effect of multiple plant components (including alkaloids, volatile oils, and tannins). Experimental data shows that this composition has a significant inhibitory effect on pathogenic bacteria and does not exhibit the unbalanced antibacterial spectrum seen in comparative example 10 / 11. The broad-spectrum, highly effective, and balanced antibacterial effect achieved by this complete formulation is unattainable by the comparative examples. This "broad-spectrum and balanced" antibacterial property helps promote the restoration of scalp microecological homeostasis. This fully demonstrates the inventiveness and technological advancement of this invention.

[0160] Table 3. Results of antibacterial performance test

[0161]

[0162] Experiment 3. Detection of scalp condition and microecology

[0163] Volunteers were recruited through a questionnaire screening method. Based on the pre-set inclusion and exclusion criteria, factors that might interfere with the experimental results, such as recent (usually within 1 to 6 months) use of other anti-hair loss shampoos, hair growth drugs, or the presence of related scalp diseases, were excluded, and finally qualified volunteer subjects were determined.

[0164] The experiment lasted for 12 weeks. On day 1, the subjects underwent their first baseline sampling and testing (denoted as T0). Subsequently, the subjects received a standardized sample of anti-hair loss shampoo and were instructed to strictly adhere to their daily shampooing routine and not to change or use other shampoo products throughout the entire trial period. After 12 weeks of use (denoted as T...), the subjects... 12 The same sampling points of the same subject were resampled and tested. The scalp health of volunteers was comprehensively assessed by three indicators: hair loss count, scalp moisture content, and sebum content.

[0165] 1. Hair Loss Count: A direct counting method was used. Each participant was given a standardized comb and instructed to comb their hair a fixed number of times (e.g., 60 times) at a fixed time each day (e.g., early morning). The comb and all fallen hair were collected and counted under sterile conditions. To reduce diurnal fluctuations, counts were performed for three consecutive days, and the arithmetic mean was taken as the time point (T0: days 1-3; T...). 12 The final amount of hair loss (days 82-84).

[0166] 2. Scalp Moisture Content: Measured using a Corneometer CM825 skin moisture meter. Several representative areas of the subject's scalp, including the top, temporal (lateral), and occipital regions, were selected as measurement points. Each measurement point was measured three times, and the average result was used to represent the skin moisture content at that point.

[0167] 3. Sebum content: Sebumeter SM815 sebum meter was used for measurement using the oil-absorbing paper method. The main measurement area was the T-zone (the T-shaped area formed by the forehead and nose) where sebum secretion is high. Each measurement point was measured three times, and the average value was taken.

[0168] 4. Under constant temperature and humidity conditions, scalp microbial samples were obtained from volunteers using a sterile swab method. The specific procedure was as follows: 24 hours after the volunteer washed their hair, a pre-selected scalp area was selected. A sterile cotton swab, moistened with sterile saline, was repeatedly swabbed 5-10 times with moderate pressure. After collection, the swab was immediately placed in a sterile tube, flash-frozen, and transported to a 4°C freezer for storage. It was then sent to a testing institution for analysis within 24 hours to preserve the original state of the microbial community to the greatest extent possible, thereby improving the accuracy of DNA extraction and high-throughput sequencing analysis.

[0169] To ensure the accuracy, repeatability, and comparability of the measurement data to the greatest extent possible, all measurements were conducted in a constant temperature and humidity environment (temperature 20±2°C, relative humidity 50±10%). In addition, subjects were required not to use any hair care products such as conditioner or hair oil within 24 hours before each measurement, and to sit quietly and balance in the experimental environment for at least 30 minutes before the measurement.

[0170] The results are shown in Tables 4 and 5. Figure 1 As shown.

[0171] Table 4. Volunteer's hair loss count, moisture level, and oil content.

[0172]

[0173] Table 5. Anti-dandruff and antipruritic effects of the composition and 5α-reductase inhibition rate

[0174]

[0175] Results analysis:

[0176] 1. Anti-hair loss effect of the composition

[0177] The compositions of the present invention shown in Examples 1-5 have a significant effect on improving hair loss (average hair loss reduction rate: 70% in Examples, 43% in Comparative Examples). Among them, the analysis of the difference in hair loss count before and after the trial of Examples 1-5 and Comparative Examples 1-3, 20, 24, and 25 shows that the volunteers' scalp moisture increased and oil content decreased, indicating that component B has a certain synergistic effect on preventing hair loss, which can enhance the effectiveness of plant ingredients and achieve a good oil control and moisturizing effect.

[0178] Comparative analysis of experimental data from Example 2 and Comparative Examples 4-14, 1-3, and 21-25 shows that the compound plant composition of the present invention (Example 2) achieved an anti-hair loss effect of up to 81%, demonstrating a significant hair growth promoting effect. Comparative Examples 4-14 systematically replaced the single plant component in component A with pure water, and the results consistently showed a decrease in anti-hair loss effect to varying degrees (30%-70%). In particular, when *Swertia japonica* (Comparative Example 5, effect reduced to 30%), *Polygonum multiflorum* (Comparative Example 6, effect 25%), or ginger root (Comparative Example 7, effect 36%) were replaced, the efficacy was significantly weakened, confirming that these components are key active ingredients for anti-hair loss activity. The anti-hair loss effects of Comparative Example 1 (without component B) and Comparative Examples 2-3 (unbalanced or diluted component B ratio) (59%, 50%, 56%) were significantly lower than those of the complete formulation examples, indicating that the optimized component B is not only a carrier but also effectively enhances the efficacy of the active ingredients. Crucially, the anti-hair loss effects of Comparative Examples 21-23 (simplified plant combination) and 24-25 (lacking core supplementary components) (24%-56% and 17%-35%) were significantly lower than those of Examples 1-5 (60%-81%). Existing technologies use fermentation products such as yeast / Acetobacter xylinum / black tea fermentation products, galactosomalidone fermentation product filtrate, and Lactobacillus / soybean extract fermentation product filtrate as active ingredients to regulate the microecology (Comparative Examples 15 and 16). Test results show that they are not as effective as the composition of this invention in preventing hair loss. This series of data fully demonstrates that there is a clear synergistic effect between the complete plant component combination (component A) and the specific chemical auxiliary component (component B). The two together constitute an inseparable technical whole, which is a necessary condition for achieving a highly effective anti-hair loss effect.

[0179] 2. Oil-controlling and moisturizing effects of the composition

[0180] Based on the comprehensive experimental data analysis of Examples 1-5 and Comparative Examples 1-25, it is shown that the compound plant composition of the present invention exhibits significant and balanced comprehensive effects in improving scalp moisture, controlling scalp oil and inhibiting 5α-reductase activity.

[0181] After using Example 1, scalp moisture increased to 149.89 (left) and 172.19 (right), oil content decreased by approximately 45%, and the 5α-reductase inhibition rate reached 43.10%, demonstrating excellent multi-target care effects. In contrast, Comparative Example 1 (without component B) showed significant decreases in all aspects: increased moisture (74.23 / 69.18), reduced oil (27.18% / 33.47%), and 5α-reductase inhibition rate (37.80%), confirming that component B plays a key role in enhancing the penetration and efficacy of active ingredients. Particularly important, Comparative Examples 4-14 systematically replaced the single plant components in component A with pure water. The results showed that when Japanese swert (Comparative Example 5, oil reduction only 18.59%), Polygonum multiflorum (Comparative Example 6, oil reduction 10.86%), or ginger root (Comparative Example 7, oil reduction 8.82%) were replaced, the oil-controlling effect was significantly weakened, confirming that these components are the core source of efficacy for achieving highly effective oil regulation. The 5α-reductase inhibition rate of Comparative Examples 21-23 (simplified plant combinations) (18.20%-25.98%) was significantly lower than that of the complete formulation examples (41.32%-47.20%), while Comparative Examples 24-25 (lacking core ingredients such as Polygonum multiflorum and Swertia japonica) showed unsatisfactory effects in terms of moisture retention and oil control. These key differences demonstrate a clear synergistic effect between the complete plant ingredient combination (component A) and specific chemical auxiliary components (component B). Together, they constitute a multi-target, comprehensive scalp health regulation system, achieving a highly effective hair loss prevention effect that cannot be achieved with a single component or simplified formulation.

[0182] 3. The anti-dandruff and antipruritic effects of the composition

[0183] Analysis of experimental data from Examples 1-5 and Comparative Examples 1-25 on itch relief and dandruff removal shows that the compound plant composition of the present invention exhibits a significant and stable effect in improving scalp comfort.

[0184] Examples 1-5 all achieved pruritus scores of 7 or higher (maximum 9 points) and dandruff removal scores of 9 points (the maximum score), demonstrating excellent overall care effects. In contrast, Comparative Example 1 (without Component B) showed a dandruff removal score of 6 points, proving that Component B made a significant contribution to improving the dandruff removal effect. Comparative Examples 4-14 showed a significant decrease in dandruff removal effect after systematically replacing the single plant component in Component A with pure water. This was particularly evident when *Swertia japonica* (Comparative Example 5, dandruff removal score 5 points), *Polygonum multiflorum* (Comparative Example 6, dandruff removal score 5 points), or ginger root (Comparative Example 7, dandruff removal score 4 points) were replaced, significantly weakening the dandruff removal effect and confirming that these components are key elements for achieving highly effective dandruff removal. Importantly, Comparative Examples 17-18 (containing only chemical dandruff removal components) had significantly lower pruritus and dandruff removal scores (both 2 and 5 points) than the examples with the complete formulation, while the effects of Comparative Examples 21-23 (simplified plant combination) (4-6 points) were also comprehensively inferior to the examples. This key gap demonstrates that there is a clear synergistic effect between the complete combination of plant ingredients (component A) and specific chemical auxiliary components (component B). Together, they constitute a multi-target scalp health regulation system, achieving a lasting anti-itch and thorough dandruff removal effect that cannot be achieved by a single chemical antidandruff agent or a simplified plant-based formula.

[0185] 4. The scalp microecological regulation effect of the composition

[0186] Maintaining a healthy scalp microbiota is crucial for hair follicle health. Malassezia is a major symbiotic fungus on the scalp, but its excessive proliferation is closely associated with dandruff and seborrheic dermatitis, thus triggering or exacerbating hair loss problems. Data shows... Figure 1 In many pre-conception (day 0) samples, the relative abundance of Malassezia was generally above 0.80. Besides fungi, the balance of the bacterial community is equally important. Key bacterial targets of interest in this invention include, but are not limited to, Staphylococcus aureus. Staphylococcus Many pathogenic Staphylococcus species are associated with scalp inflammation and hair follicle infections, and a decrease in their relative abundance is a direct indicator of the composition's ability to modulate pathogenicity. Dermatobacteria ( Cutibacterium ): Related to sebum secretion and acne formation, its balance is crucial for a healthy sebum environment. Probiotics (such as lactobacilli) Lactobacillus and Bifidobacteria Bifidobacterium The maintenance or growth of these microbial communities is key evidence that the composition has the ability to "selectively regulate" rather than simply "indiscriminately kill".

[0187] The compositions of the present invention (Examples 1-5) all exhibited significantly better inhibitory effects than the comparative Malassezia, and demonstrated broad-spectrum and balanced microbial community regulation characteristics through the synergistic effect of multiple plant components.

[0188] Example 1:

[0189] After 84 days of continuous use, Malassezia ( Malassezia The relative abundance of Staphylococcus aureus decreased from 0.8707 before use to 0.2247, with an inhibition rate as high as 74.2%. This reduction demonstrates the high inhibitory capacity of the composition against the core pathogenic fungi of the scalp microecology. Regarding the bacterial community, the regulatory effect of Example 1 showed high selectivity: Staphylococcus aureus (… Staphylococcus ) and Lawsonia ( Lawsonella Complete elimination of Staphylococcus aureus: The relative abundance of Staphylococcus aureus plummeted from 0.8728 to 0.0242, achieving an inhibition rate of 97.2%. Simultaneously, the abundance of Lawsonia lactiflora dropped completely from 0.0868 to 0, demonstrating a powerful ability to eliminate inflammatory indicator bacteria. This indicates that the composition has a strong controlling effect on potential scalp pathogens, particularly Staphylococcus aureus, which can cause inflammation and infection. Significant promotion of probiotic flora: Lactobacillus (… Lactobacillus The relative abundance of Bifidobacterium increased significantly from 0.0006 to 0.0123; Bifidobacterium The number of bacteria significantly increased from extremely low levels (2.53e-05) to 0.0121. This indicates that the composition has excellent selective regulatory capabilities. Symbiotic community dynamics: Acinetobacter (… Acinetobacter The relative abundance increased from 0.0002 to 0.0124, Bacteroides ( Bacteroides The concentration also increased from 0.00098 to 0.0313. After the pathogens were eliminated, these symbiotic bacteria occupied their niches and proliferated, reflecting the reshaping and rebalancing of the macro-microbial niches of the scalp. This phenomenon indicates that while the composition strongly inhibits pathogenic fungi and bacteria, it creates a favorable growth environment for beneficial bacteria, embodying the composition's concept of "regulating" rather than "completely eradicating" microecological balance.

[0190] Examples 2-3:

[0191] Examples 2 and 3 are fine-tuned formulations based on Example 1, designed to explore the optimal range of efficacy. Example 2: The proportions of the herbal ingredients in Component A were slightly adjusted (Phellodendron bark extract increased to 12 parts, Coptis root extract increased to 10 parts, and Platycladus orientalis leaf decreased to 18 parts). This formulation still showed excellent inhibitory effects against Malassezia, decreasing from 0.8950 to 0.2270, with an inhibition rate as high as 74.6%. The inhibition rate against Staphylococcus aureus also reached 89.9% (decreasing from 0.9051 to 0.0911). Example 3: The amount of Component A was generally reduced, and the surfactant proportions in Component B were slightly changed (sodium cocamidopropyl-PG-dimethylammonium chloride phosphate decreased to 75 parts, and sodium isostearyl lactylate increased to 20 parts). Despite the reduced amount of Component A, its inhibition rate against Malassezia still reached 67.1% (decreasing from 0.8744 to 0.2875), demonstrating excellent efficacy. More notably, Example 3 achieved the strongest Staphylococcus aureus inhibition effect among all formulations, with the relative abundance decreasing from 0.6877 to 0.0144, resulting in an inhibition rate of up to 97.9%; it also significantly promoted the proliferation of Lactobacillus (0.0135) and Bifidobacterium (0.0115).

[0192] Examples 1, 2, and 3 together define the ideal range of the composition of the present invention for highly efficient regulation of the microecology, namely, the value of Malassezia is below 0.30 and the inhibition rate of Staphylococcus is above 89%.

[0193] Examples 4-5:

[0194] Examples 4 and 5 demonstrate the sensitivity of formulation boundaries to efficacy by adjusting the component ratios of components A and B. Example 4: The amount of herbal ingredients in component A was generally increased, but the dichlorobenzyl alcohol in component B was reduced to 3 parts. This formulation significantly reduced the inhibition rate against Malassezia to 41.1% (from 0.9080 to 0.5350). Although the inhibition effect against Staphylococcus aureus remained excellent (94.7%), the control effect against Malassezia was significantly worse than in Examples 1-3. This result indicates that even increasing the total amount of plant extracts in component A can lead to a significant decrease in overall antifungal efficacy if the proportion of specific components in component B (such as dichlorobenzyl alcohol) is below the optimal value. This strongly supports a precise synergistic dependence between the active components in components A and B. Example 5: Component A used the standard formulation, but the dichlorobenzyl alcohol in component B was increased to 7 parts. The inhibition rate of Malassezia decreased to 53.6% (from 0.8965 to 0.4158), and the inhibition rate of Staphylococcus aureus was only 69.7% (from 0.8018 to 0.2431). Increasing the content of dichlorobenzyl alcohol did not lead to enhanced inhibition; instead, it weakened the overall regulatory capacity against Malassezia and Staphylococcus aureus. This further confirms that the optimal effect of this invention does not come from the highest concentration of a single component, but rather from multiple synergistic balances achieved through a precise ratio of component A to component B.

[0195] Comparative Examples 1-3:

[0196] Comparative Example 1 contained only the plant extract of Component A, without the surfactant and dichlorobenzyl alcohol of Component B. This formulation showed an inhibition rate of only 43.5% against Malassezia (decreased from 0.6188 to 0.3498) and an inhibition rate of 69.7% against Staphylococcus aureus (decreased from 0.3342 to 0.1013). Comparing Comparative Example 1 (Component A only) with Example 1 (Component A + Component B), the Malassezia inhibition rate of Example 1 (74.2%) was found to be 1.7 times that of using Component A alone. This directly demonstrates that the addition of Component B does not merely provide basic washing function, but produces a significant synergistic effect, maximizing the activity of Component A. This synergistic effect is the key innovation of this invention. Comparative Example 2 maintained the 10:1 A / B ratio, but adjusted the surfactant ratio within Component B. Sodium cocamidopropyl-PG-dimethylammonium chloride phosphate (80 parts) was reduced to 50 parts, while sodium isostearyl lactylate (15 parts) was increased to 45 parts. This imbalance resulted in a significant decrease in the inhibitory effect against Malassezia, with an inhibition rate of only 38.0% (from 0.9166 to 0.5677). Comparative Example 3 involved diluting the active ingredient by adding 30 parts of purified water to component B. The diluted composition showed a decrease in the inhibition rate against Malassezia to 34.1% (from 0.8228 to 0.5415).

[0197] These results clearly indicate that even if the composition of component A remains unchanged, the proportion of active excipients within component B, as well as the overall concentration of A and B, must be precisely controlled within the ranges of Examples 1-5 to ensure its efficient microecological regulation ability. More importantly, any adjustment to component B (change in the weight ratio of Comparative Example 2 or dilution in Comparative Example 3) will lead to a significant decrease in core antifungal activity and trigger a chain reaction in the macro-microecology, such as the Clostridium species in Comparative Example 2 (…). Clostridium_ in the strict sense_1 The explosive proliferation of the relative abundance of component B highlights the fine-tuning ability of the formulation in regulating the stability of the micro-ecosystem. This suggests that an imbalance in the proportion of component B may lead to severe niche disruption and instability of the scalp microbiota.

[0198] Comparative Example 4-14:

[0199] Comparative Examples 4 to 14 verified the necessity of each herb for the overall efficacy by sequentially replacing any one of the 11 herbs in Component A of Example 1 with an equal amount of pure water. The results showed that all 11 herbs in Component A are an indispensable part of the highly efficient microecological regulation system. The inhibitory effect of Malassezia significantly decreased or completely failed after the absence of any of the following components (the relative abundance of Malassezia in the later stages of the trial was concentrated between 0.60 and 0.77, far higher than 0.2247 in Example 1): Comparative Example 4 (without Platycladus orientalis leaves): Malassezia value reached as high as 0.6876; Comparative Example 5 (without Swertia japonica): Malassezia value reached as high as 0.6880, and the inhibition of Staphylococcus aureus also failed significantly, indicating that this component contributes to the dual regulation of fungi and bacteria; Comparative Example 6 (without Polygonum multiflorum): Malassezia value reached as high as 0.7399, although the inhibition of Staphylococcus aureus was still acceptable, the ecological niche imbalance was severe; Comparative Example 9 (without Zanthoxylum bungeanum pericarp): Malassezia value reached as high as 0.7709. These data demonstrate that Japanese swert, arborvitae leaves, Polygonum multiflorum, and Sichuan pepper pericarp are crucial for maintaining a highly effective antifungal synergistic effect in the compound system.

[0200] Comparative Examples 6 (lacking Polygonum multiflorum) and 13 (lacking Coptis chinensis) provide important evidence regarding the separation of the active mechanisms. Comparative Example 6: Malassezia inhibition failed (post-value 0.7399), but the inhibition of Staphylococcus aureus was extremely excellent (decreased from 0.0996 to 0.0080, inhibition rate 92.0%). Comparative Example 13: Malassezia inhibition failed (post-value 0.6806), and the inhibition of Staphylococcus aureus was also excellent (decreased from 0.0079 to 0.0076; due to the low starting value, the rate of change was small, but controlled at an extremely low level). These two cases suggest that Polygonum multiflorum and Coptis chinensis may primarily participate in the synergistic inhibition mechanism against Malassezia, while the inhibition of Staphylococcus aureus is mainly undertaken by other components in fraction A or fraction B.

[0201] Comparative Example 14 showed that the later value of Malassezia decreased to 0.1069, which appeared to be superior to Example 1 (0.2247). However, the relative abundance of Malassezia in this formulation was extremely low in the early stages of use, at only 0.1935, compared to 0.8707 in Example 1. Due to the significant difference in the starting baseline, this "superiority" at a single data point is not comparable and cannot refute the synergistic necessity of the other 10 herbs for maintaining the overall microecological balance.

[0202] In summary, the results of Comparative Examples 4-14 collectively demonstrate the core innovation of this invention: the 11 herbs in component A constitute an indivisible synergistic compound system. The absence of any single component leads to a significant degradation in overall regulatory efficacy, and is usually accompanied by an imbalance in Staphylococcus regulation or the overall fungal community (such as fungi and cladocerans). This synergistic effect is not obvious and transcends the simple summation of the effects of individual herbs.

[0203] Comparative Examples 15-16:

[0204] Comparative Examples 15 and 16 replaced most of the herbal components in component A with different bio-fermentation products. Comparative Example 15 (containing yeast / Acetobacter xylinum / black tea fermentation product): Malassezia inhibition rate was only 9.6% (decreased from 0.8694 to 0.7854), while Broutella increased (from 0.005 to 0.012); Comparative Example 16 (containing Lactobacillus / soybean extract fermentation product filtrate): Malassezia inhibition rate was only 4.1% (decreased from 0.8466 to 0.8119), and various fungi (Penicillium, Aspergillus) were uncontrolled. This demonstrates that the chemical structure and multi-target mechanism of component A of the plant compound extract cannot be replaced by simple bio-fermentation products.

[0205] Compared to the 74.2% inhibition rate of Example 1, the bio-fermentation product almost completely failed in inhibiting Malassezia. This comparison strongly demonstrates that the chemical structure and multi-target mechanism of action of component A of the compound plant extract of this invention are key to achieving efficient microecological regulation and cannot be easily replaced by common microbial fermentation products.

[0206] Comparative Examples 17-20:

[0207] Comparative Example 17 (containing 5 parts OCT): The relative abundance of Malassezia decreased from 0.8239 to 0.1912, with an inhibition rate of 76.7%, indicating a moderate effect. Comparative Example 18 (containing 5 parts ZPT): After using ZPT, the relative abundance of Malassezia decreased from 0.8408 to 0.1517, with an inhibition rate of 81.9%, but the relative abundance of Staphylococcus aureus also increased from 0.0835 to 0.0966 (an increase of 15.7%). Comparative Example 19 (blank shampoo): In a matrix without any added active ingredients, the relative abundance of Malassezia surged from 0.1248 to 0.8166; Staphylococcus aureus also surged from 0.0406 to 0.1982. This confirms that the scalp microecology is easily imbalanced in the absence of regulation and further demonstrates that the matrix itself cannot achieve microecological regulation. Comparative Example 20 (component B only): This formulation is the same as component B of Example 1, but lacks component A. The relative abundance of Malassezia increased from 0.5414 to 0.7822 (an increase of 44.5%), while Lawsonella surged from 0.0274 to 0.3793. These comparative data indicate that Examples 1-5, compared to traditional antifungal agents OCT and ZPT, exhibited similar Malassezia inhibition rates over the same time span. However, ZPT even led to an increase in the relative abundance of Staphylococcus aureus, and the antimicrobial agent dichlorobenzyl alcohol in component B, without the synergistic effect of component A, could not provide effective antifungal or balanced microecological efficacy on its own, and might even disrupt the balance. This suggests that existing antifungal agents (dichlorobenzyl alcohol, OCT, ZPT, etc.) may not effectively regulate the scalp microecology under specific shampoo bases and long time spans, and may even exacerbate the imbalance. The composition of this invention achieves simultaneous and highly efficient inhibition of Malassezia and Staphylococcus aureus, further emphasizing the synergistic effect of the specific ratio of components A and B, significantly surpassing existing technologies.

[0208] Comparative Examples 21-25:

[0209] Comparative Examples 21, 22, and 23 were designed to test the activity of a small number of core herbs in component A. For example, Comparative Example 23 retained only four herbs: ginger root, Polygonum multiflorum, Swertia japonica, and Platycladus orientalis leaves, and mixed them with standard component B. Results of Comparative Example 23: The relative abundance of Malassezia increased dramatically from 0.4118 to 0.8934 (an increase of 117.0%), and Enhydrobacterium increased dramatically from 0.00003 to 0.0637. Results of Comparative Example 25: When component A (lacking ginger root, Polygonum multiflorum, Swertia japonica, and Platycladus orientalis leaves) was mixed with component B, the relative abundance of Malassezia increased dramatically from 0.1717 to 0.8209 (an increase of 378.0%). These results clearly demonstrate that the 11 herbs in component A constitute a complex compound system highly dependent on integrity. The severe simplification of the formulation led to uncontrolled Malassezia and an outbreak of opportunistic pathogens (such as hydrophilic bacteria), indicating that even with the retention of several core herbs (such as in Comparative Example 23), the simplification completely undermined the efficacy of microecological regulation, resulting in uncontrolled Malassezia. This further demonstrates the uniqueness and optimality of the formulations in the examples.

[0210] The test results above indicate that mixing component A (11 herbs) with component B at a weight ratio of 10:1 produces a non-obvious synergistic effect. This synergistic effect not only improves the bioavailability of active substances, but more importantly, it creates a special environment that can achieve dual, highly effective inhibition and selective balance of the scalp microecology.

[0211] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Therefore, any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A composition for modulating the scalp microflora, characterized in that, The composition consists of A component and B component, wherein the A component consists of the following components by weight fraction: Phellodendron bark extract 5-15 parts, Coptis root extract 3-14 parts, Sophora root extract 5-15 parts, Rhus gallaenoidea extract 2-8 parts, Cnidium fruit extract 2-18 parts, Zanthoxylum pericarp extract 3-10 parts, Cinnamomum root extract 1-5 parts, Ginger root extract 3-12 parts, Polygonum multiflorum root extract 5-15 parts, Japanese Sabina extract 5-25 parts, Platycladus orientalis leaf extract 10-30 parts; The B component consists of the following components by weight fraction: Sodium cocamido propyl-PG-dimethyl ammonium chloride phosphate ester 70-90 parts, sodium isostearoyl lactylate 10-30 parts, dichlorobenzyl alcohol 1-10 parts; The weight ratio of the A component to the B component is 10:1; In the Phellodendron bark extract, the content of phellodendrine is ≥3000ppm, in the Coptis root extract, the content of berberine is ≥1000ppm, in the Sophora root extract, the content of sophoramine is ≥3000ppm, in the Rhus gallaenoidea extract, the content of gallic acid is ≥3000ppm, in the Cnidium fruit extract, the content of cnidim is ≥3000ppm, in the Zanthoxylum pericarp extract, the content of naringin is ≥2000ppm, in the Cinnamomum root extract, the content of camphor is ≥1000ppm, in the Ginger root extract, the content of gingerol is ≥3000ppm, in the Polygonum multiflorum root extract, the content of emodin is ≥1000ppm, in the Japanese Sabina extract, the content of sabina is ≥2000ppm, and in the Platycladus orientalis leaf extract, the content of quercitrin is ≥1000ppm.

2. The composition of claim 1, wherein, The A component consists of the following components by weight fraction: Phellodendron bark extract 8-12 parts, Coptis root extract 5-10 parts, Sophora root extract 8-12 parts, Rhus gallaenoidea extract 3-6 parts, Cnidium fruit extract 5-16 parts, Zanthoxylum pericarp extract 5-8 parts, Cinnamomum root extract 2-4 parts, Ginger root extract 5-8 parts, Polygonum multiflorum root extract 8-12 parts, Japanese Sabina extract 7-18 parts, and Platycladus orientalis leaf extract 16-24 parts.

3. The composition of claim 2, wherein, The A component consists of the following components by weight fraction: Phellodendron bark extract 8-12 parts, Coptis root extract 5-10 parts, Sophora root extract 8-10 parts, Rhus gallaenoidea extract 3-5 parts, Cnidium fruit extract 5-10 parts, Zanthoxylum pericarp extract 5-7 parts, Cinnamomum root extract 2-4 parts, Ginger root extract 5-8 parts, Polygonum multiflorum root extract 8-12 parts, Japanese Sabina extract 7-16 parts, and Platycladus orientalis leaf extract 16-20 parts.

4. The composition of claim 3, wherein, The A component consists of the following components by weight fraction: Phellodendron bark extract 8-12 parts, Coptis root extract 5-10 parts, Sophora root extract 8-10 parts, Rhus gallaenoidea extract 3-5 parts, Cnidium fruit extract 5-10 parts, Zanthoxylum pericarp extract 5-7 parts, Cinnamomum root extract 2-4 parts, Ginger root extract 5-8 parts, Polygonum multiflorum root extract 8-12 parts, Japanese Sabina extract 7-16 parts, and Platycladus orientalis leaf extract 16-20 parts. The A component consists of the following components by weight fraction: Phellodendron bark extract 10 parts, Coptis root extract 8 parts, Sophora root extract 10 parts, Rhus gallaenoidea extract 4 parts, Cnidium fruit extract 10 parts, Zanthoxylum pericarp extract 7 parts, Cinnamomum root extract 3 parts, Ginger root extract 6 parts, Polygonum multiflorum root extract 10 parts, Japanese Sabina extract 13 parts, and Platycladus orientalis leaf extract 20 parts.

5. The composition of claim 1, wherein, The B component consists of the following components by weight fraction: sodium cocamidopropyl-PG-dimethyl ammonium chloride phosphate ester 75-85 parts, sodium isostearoyl lactylate 12-20 parts, dichlorobenzyl alcohol 3-7 parts.

6. The composition of claim 5, wherein, The B component consists of the following components by weight fraction: sodium cocamidopropyl-PG-dimethyl ammonium chloride phosphate ester 75-80 parts, sodium isostearoyl lactylate 15-20 parts, dichlorobenzyl alcohol 5 parts.

7. The composition of claim 6, wherein, The B component consists of the following components by weight fraction: sodium cocamidopropyl-PG-dimethyl ammonium chloride phosphate ester 75-80 parts, sodium isostearoyl lactylate 15-20 parts, dichlorobenzyl alcohol 5 parts.

8. Use of the composition according to any one of claims 1 to 7 in the manufacture of a cosmetic product for regulating the microecological balance of the scalp.

9. Use of the composition according to any one of claims 1 to 7 in the manufacture of a cosmetic product for oil control, dandruff removal, relief of head itch, and hair loss prevention.

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