A composition containing PDRN with firming and anti-wrinkle efficacy and application thereof

Abstract

The application belongs to the field of daily chemicals, and discloses a composition containing PDRN and having the effect of tightening and anti-wrinkle, wherein the composition comprises PDRN, an extract of Saururus chinensis, a root oil of Phlox subulata, a seed oil of Rosa rubus, and a seed oil of Panax ginseng. The composition provided by the application uses PDRN, the extract of Saururus chinensis, the root oil of Phlox subulata, the seed oil of Rosa rubus, and the seed oil of Panax ginseng in combination, and through mutual cooperation of the components, synergistically promotes the secretion of collagen type I, so that the composition has the effect of tightening and anti-wrinkle. In addition, the composition provided by the application also has the effect of barrier repair, can significantly down-regulate the TEWL value of skin, is mild and non-irritating, and is suitable for people with sensitive skin.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of daily cosmetics, in particular to a composition containing PDRN with firming and anti-wrinkle effects and application thereof. BACKGROUND

[0002] Skin aging is a complex physiological process, mainly manifested as wrinkle formation, skin laxity, loss of elasticity, etc. Its internal mechanism is closely related to the decrease in synthesis and increase in degradation of extracellular matrix in the dermis, especially type I collagen. Therefore, promoting the production of type I collagen is one of the key ways to achieve firming and anti-wrinkle effects in the field of cosmetics.

[0003] At present, a variety of active ingredients with anti-wrinkle effects have been widely used on the market, such as retinol and its derivatives, vitamin C, polypeptides, fruit acids, etc. However, these ingredients have certain limitations in practical application. Retinol has a significant effect, but it is highly irritating and can cause adverse reactions such as dry skin, redness, and desquamation, which is not suitable for long-term use by sensitive skin people; vitamin C is unstable in nature and is easily oxidized; and the cost of some synthetic polypeptides is high, and the transdermal absorption efficiency needs to be improved.

[0004] On the other hand, many natural extracts, such as various plant essential oils and extracts, are favored due to their mildness and safety. They usually contain rich antioxidant and anti-inflammatory ingredients, which help to delay skin aging. However, the efficacy of a single natural ingredient in promoting collagen regeneration is often limited, and it is difficult to achieve the desired firming and anti-wrinkle effect. If multiple natural ingredients are compounded, the interaction between different ingredients is complex, and simple addition may not produce a synergistic effect, and may even result in mutual offsetting of efficacy or increased irritation.

[0005] Therefore, there is an urgent need in the art to develop a new composition that can effectively promote the secretion of type I collagen, improve wrinkles and laxity problems from the root, and at the same time has good skin barrier repair function, is mild, and can meet the safety skin care needs of a wider range of people including sensitive skin. SUMMARY

[0006] Based on the deficiencies of the prior art described above, the present application aims to provide a firming and anti-wrinkle composition. The firming and anti-wrinkle composition provided by the present application has a synergistic effect between the components, and the use of the components together can significantly promote the secretion of type I collagen, reduce the skin TEWL value, and is mild and non-irritating, suitable for sensitive skin people.

[0007] In a first aspect, the present application provides a composition containing PDRN with firming and anti-wrinkle effects, which comprises the following components by mass:

[0008] PDRN: 0.01-0.2 parts;

[0009] Saururus chinensis extract: 0.1-0.3 parts;

[0010] Chrysopogon zizanioides root oil: 0.1-0.3 parts;

[0011] Rosarubiginosa seed oil: 2-4 parts;

[0012] Panax ginseng seed oil: 0.1-0.3 parts.

[0013] The composition provided by the present application includes PDRN, which is also known as polydeoxyribonucleotide. PDRN can be derived from animals, plants, microorganisms, and biosynthesis. PDRN has significant effects of tightening and anti-wrinkling, barrier repair, anti-inflammatory, improving pigmentation, and promoting wound healing in skin care products.

[0014] The composition provided by the present application includes Saururus chinensis extract, which is extracted from traditional Chinese medicine Saururus chinensis (Lour.) Baill. Saururus chinensis extract is rich in lignin (such as Saururus chinensis ketone) and triterpenoid active substances, and has significant effects of anti-inflammatory, antioxidant, oil control, and antibacterial.

[0015] The composition provided by the present application includes Chrysopogon zizanioides root oil, which is extracted from the roots of Chrysopogon zizanioides (L.) Roberty. Chrysopogon zizanioides root oil is rich in terpenoid active substances such as chrysopogonol, chrysopogon ketone, and chrysopogonene, and has effects of sterilization and anti-inflammatory, promoting wound healing, shrinking pores, and promoting skin cell proliferation.

[0016] The composition provided by the present application contains Rosarubiginosa seed oil, which is extracted from the seeds of Rosarubiginosa. Rosarubiginosa seed oil is rich in active substances such as linoleic acid, linolenic acid, gamma-linolenic acid, vitamin A, vitamin E, and carotenoids, and has significant effects of anti-aging, soothing, anti-inflammatory, repair, and moisturizing.

[0017] The composition provided by the present application contains Panax ginseng seed oil, which is extracted from the seeds of Panax ginseng C. A. Mey. Panax ginseng seed oil is rich in active substances such as unsaturated fatty acids, oleic acid, ginsenosides, and vitamin E, and has significant effects of antioxidant, anti-aging, moisturizing, barrier repair, skin lightening, and sun protection.

[0018] The composition provided by the present application uses PDRN, threeleaf cudweed extract, orchid root oil, rust red rose seed oil and ginseng seed oil in combination, and each component cooperates and promotes each other, and synergistic effect is obtained in promoting type I collagen secretion, so that the composition has the effect of tightening and anti-wrinkling. In addition, the composition provided by the present application can also significantly down-regulate the skin TEWL value, has the skin barrier repair effect, and is mild and non-irritating, and is suitable for sensitive skin people.

[0019] The inventors found in actual research that the mass fraction of PDRN, threeleaf cudweed extract, orchid root oil, rust red rose seed oil and ginseng seed oil in the composition of the composition will affect the tightening and anti-wrinkling and repair effects of the composition, and when the mass fraction of PDRN, threeleaf cudweed extract, orchid root oil, rust red rose seed oil and ginseng seed oil is within the above range, the tightening and anti-wrinkling and repair effects are better.

[0020] Preferably, the composition comprises the following mass fraction of components:

[0021] PDRN: 0.1-0.2 parts;

[0022] Threeleaf cudweed extract: 0.2-0.3 parts;

[0023] Orchid root oil: 0.2-0.3 parts;

[0024] Rust red rose seed oil: 3-4 parts;

[0025] Ginseng seed oil: 0.2-0.3 parts.

[0026] The inventors found in actual research that the mass fraction of PDRN, threeleaf cudweed extract, orchid root oil, rust red rose seed oil and ginseng seed oil in the composition of the composition will affect the tightening and anti-wrinkling and repair effects of the composition, and when the mass fraction of PDRN, threeleaf cudweed extract, orchid root oil, rust red rose seed oil and ginseng seed oil is within the above range, the tightening and anti-wrinkling and repair effects are better.

[0027] Preferably, the composition comprises the following mass fraction of components:

[0028] PDRN: 0.1 parts;

[0029] Threeleaf cudweed extract: 0.2 parts;

[0030] Orchid root oil: 0.2 parts;

[0031] Rust red rose seed oil: 3 parts;

[0032] Ginseng seed oil: 0.2 parts.

[0033] The inventors found in actual research that the mass fraction of PDRN, threeleaf cudweed extract, iris root oil, rust red rose seed oil and ginseng seed oil in the composition can affect the firming and anti-wrinkle and repair effects of the composition, and the firming and anti-wrinkle and repair effects are better when the mass fraction of PDRN, threeleaf cudweed extract, iris root oil, rust red rose seed oil and ginseng seed oil is as described above.

[0034] In a second aspect, the present application provides use of the PDRN-containing composition with firming and anti-wrinkle effects according to the first aspect in the preparation of a cosmetic product with firming and anti-wrinkle effects.

[0035] Preferably, the dosage form of the cosmetic product is at least one of a powder, an emulsion, a cream and a spray.

[0036] In a third aspect, the present application provides a water-oil two-phase essence oil with firming and anti-wrinkle effects, wherein the essence oil comprises the PDRN-containing composition with firming and anti-wrinkle effects according to the first aspect.

[0037] Preferably, the essence oil further comprises a humectant, an emollient, a preservative and deionized water.

[0038] Preferably, the humectant is at least one of glycerin, hyaluronic acid and allantoin.

[0039] Preferably, the emollient is at least one of European hazel seed oil, caprylic / capric triglyceride, triisooctanoin, white pool seed oil, C9-12 alkane, jojoba seed oil, squalane, glass flax seed oil and oat kernel oil.

[0040] Preferably, the preservative is at least one of p-hydroxyacetophenone, 1,2-hexanediol and paraben.

[0041] Compared with the prior art, the present application has the following beneficial effects:

[0042] The present application initiatively uses PDRN, threeleaf cudweed extract, iris root oil, rust red rose seed oil and ginseng seed oil in combination to obtain a composition with firming and anti-wrinkle effects, and research shows that the composition formed by PDRN, threeleaf cudweed extract, iris root oil, rust red rose seed oil and ginseng seed oil forms a multidimensional synergistic mechanism in promoting the secretion of type I collagen and reducing the TEWL value of the skin.

[0043] In promoting the secretion of collagen type I, PDRN (polydeoxyribonucleotides) acts as a core signal molecule, playing the role of a "synthesis initiator". The mechanism is that PDRN can bind to specific receptors on the surface of fibroblast membranes (such as adenosine A2A receptors), activate intracellular signal transduction pathways, and significantly up-regulate the secretion of collagen type I. This is a direct and efficient driving signal for promoting collagen regeneration; The core function of Sanbiac Extract (rich in triterpenoids and lignin active ingredients) and Phlox Root Oil (rich in phloxol and other terpenoids) is to "clear obstacles and escort". Through their excellent antioxidant and anti-inflammatory activities, they can effectively scavenge excess reactive oxygen species generated by external stimuli such as ultraviolet light, and inhibit key signaling molecules of inflammatory pathways such as NF-κB. This action can significantly reduce the overexpression of matrix metalloproteinases, thereby reducing the degradation of synthesized collagen type I, and creating a healthy microenvironment for fibroblasts with low oxidative stress and low inflammation levels, indirectly enhancing and maintaining the synthesis-promoting effect of PDRN; Rusty red rose seed oil and ginseng seed oil serve as "nutrient sources". The unsaturated fatty acids, sterols, vitamin E, and ginsenosides contained in both can enhance cell viability and support this high-energy process. At the same time, these oil components help to consolidate the skin lipid barrier and maintain the optimal hydration environment required for normal cell metabolism, ensuring the smooth progress of the collagen synthesis process from the basic level.

[0044] In reducing the skin TEWL value, PDRN, as a cell-level repair signal molecule, can directly act on epidermal keratinocytes to promote their proliferation and differentiation. This process is crucial for maintaining the integrity of the skin barrier, as it accelerates the renewal of damaged or aged stratum corneum, helping to rebuild a dense and healthy stratum corneum structure, thereby reducing passive water loss at the cellular level; Rusty red rose seed oil and ginseng seed oil are rich in plant sterols, ceramide analogues, and unsaturated fatty acids, which can effectively penetrate the stratum corneum and supplement the composition of intercellular lipids. These exogenous lipids can fuse with the skin's own lipids, rearrange to form a more dense lamellar structure, and firmly connect keratinocytes, the "bricks", like "mortar", directly reinforcing the physical barrier of the skin and enhancing its water retention capacity; Sanbiac Extract and Phlox Root Oil provide a stable external environment for barrier repair through their anti-inflammatory and soothing properties. Micro-inflammation caused by external stimuli can disrupt the stability of the barrier and interfere with lipid synthesis and arrangement. These two components help to reduce such adverse stimuli, thereby indirectly ensuring that the repair effect of PDRN and the integration process of exogenous lipids can proceed smoothly, maintaining the homeostasis of the barrier function.

[0045] In summary, the composition provided by the present application creates and maintains a favorable "external environment" for the synthesis and persistence of collagen by strengthening the barrier (reducing the TEWL value); at the same time, it provides solid "internal support" for the barrier function by promoting collagen neogenesis. PDRN, extract of threeleaf kindeywort, vetiveria zizanoides root oil, rosa rubiginosa seed oil and panax ginseng seed oil each plays its own role and promotes each other in the system, and jointly acts on the "dermal-epidermal" functional unit, and finally realizes the truly synergistic effect beyond a single approach in tightening, anti-wrinkle and barrier repair. DETAILED DESCRIPTION

[0046] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples. The purpose is to understand the content of the present application in detail, rather than to limit the present application. All other examples obtained by those of ordinary skill in the art without making creative efforts fall within the protection scope of the present application. The experimental reagents and instruments designed in the implementation and comparative examples of the present application are common reagents and instruments unless otherwise specified, and can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and the raw materials used in parallel experiments are the same batch of raw materials unless otherwise specified.

[0047] The raw materials used in the composition will be described below, but are not limited to the following raw materials:

[0048] PDRN-1 was purchased from Regenera Life Science; extraction source: brown algae; item number: RJMLDE-C03.

[0049] PDRN-2 was purchased from Regenera Life Science; extraction source: biota orientalis; item number: RJMPOLE-C02.

[0050] PDRN-3 was purchased from Regenera Life Science; extraction source: male salmon testis; item number: RJMPDRN-C02.

[0051] The extract of threeleaf kindeywort was purchased from Shanghai Gaoke Biological Co., Ltd.; item number: Xiayanyin (Erythcalm SC).

[0052] Vetiveria zizanoides root oil was purchased from Guangzhou Zhuangyou Biological Technology Co., Ltd.; item number: Xianggenyou (Vetiveria zizanoides root oil).

[0053] Rosa rubiginosa seed oil was purchased from Northstar Lipids (UK) Ltd; item number: Rose Hip Seed Oil CB (rose fruit oil).

[0054] Panax ginseng seed oil was purchased from Guangdong Yue Microbial Technology Co., Ltd.; item number: Panax ginseng seed oil.

[0055] The remaining raw materials were obtained from the market.

[0056] The composition with PDRN, which has the firming and anti-wrinkle effect, its components, mass parts and preparation method are as follows:

[0057] Composition 1:

[0058] It is composed of the following mass parts of components:

[0059] PDRN-1: 0.1 part;

[0060] Saururus chinensis extract: 0.2 part;

[0061] Orchid root oil: 0.2 part;

[0062] Rusty red rose seed oil: 3 parts;

[0063] Ginseng seed oil: 0.2 part;

[0064] Preparation method: mix the above components uniformly, seal and store in dark.

[0065] Composition 2:

[0066] It is composed of the following mass parts of components:

[0067] PDRN-1: 0.01 part;

[0068] Saururus chinensis extract: 0.1 part;

[0069] Orchid root oil: 0.1 part;

[0070] Rusty red rose seed oil: 2 parts;

[0071] Ginseng seed oil: 0.1 part;

[0072] Preparation method: same as composition 1.

[0073] Composition 3:

[0074] It is composed of the following mass parts of components:

[0075] PDRN-1: 0.2 part;

[0076] Saururus chinensis extract: 0.3 part;

[0077] Orchid root oil: 0.3 part;

[0078] Rusty red rose seed oil: 4 parts;

[0079] Ginseng seed oil: 0.3 part;

[0080] Preparation method: same as composition 1.

[0081] Composition ①:

[0082] Different from composition 1, PDRN-2 is used to replace PDRN-1 in equal mass; the rest of the components, mass parts and the preparation method of the composition are the same as composition 1.

[0083] Composition 2:

[0084] Different from composition 1, PDRN-3 is used to replace PDRN-1 in equal mass; the rest of the components, mass parts and the preparation method of the composition are the same as composition 1.

[0085] Composition 3:

[0086] Different from composition 1, PDRN-1 is absent, and the missing mass parts are made up of Saururus chinensis extract, Vetiveria zizanioides root oil, Rosa rubus seed oil and Panax seed oil in a mass ratio of 0.2:0.2:3:0.2; the rest of the components, mass parts and the preparation method of the composition are the same as composition 1.

[0087] Composition 4:

[0088] Different from composition 1, Saururus chinensis extract is absent, and the missing mass parts are made up of PDRN-1, Vetiveria zizanioides root oil, Rosa rubus seed oil and Panax seed oil in a mass ratio of 0.1:0.2:3:0.2; the rest of the components, mass parts and the preparation method of the composition are the same as composition 1.

[0089] Composition 5:

[0090] Different from composition 1, Vetiveria zizanioides root oil is absent, and the missing mass parts are made up of PDRN-1, Saururus chinensis extract, Rosa rubus seed oil and Panax seed oil in a mass ratio of 0.1:0.2:3:0.2; the rest of the components, mass parts and the preparation method of the composition are the same as composition 1.

[0091] Composition 6:

[0092] Different from composition 1, Rosa rubus seed oil is absent, and the missing mass parts are made up of PDRN-1, Saururus chinensis extract, Vetiveria zizanioides root oil and Panax seed oil in a mass ratio of 0.1:0.2:0.2:0.2; the rest of the components, mass parts and the preparation method of the composition are the same as composition 1.

[0093] Composition 7:

[0094] Different from composition 1, Panax seed oil is absent, and the missing mass parts are made up of PDRN-1, Saururus chinensis extract, Vetiveria zizanioides root oil and Rosa rubus seed oil in a mass ratio of 0.1:0.2:0.2:3; the rest of the components, mass parts and the preparation method of the composition are the same as composition 1.

[0095] Composition 8:

[0096] Composed of the following mass parts of components:

[0097] PDRN-1: 0.3 parts;

[0098] Saururus chinensis extract: 0.1 parts;

[0099] Orpinia stricta root oil: 2.5 parts;

[0100] Rosa rubiginosa seed oil: 0.4 parts;

[0101] Panax seed oil: 0.4 parts;

[0102] Preparation method same as composition 1.

[0103] Water-oil two-phase essence oil with firming and anti-wrinkle effects, the components, mass percentages and preparation method of the essence oil are as follows:

[0104] Essence oil 1:

[0105] Consists of the following mass percentage components:

[0106] Composition 1: 5wt%;

[0107] Humectant: 1wt%;

[0108] Emollient: 20wt%;

[0109] Preservative: 0.3wt%;

[0110] Deionized water to 100wt%;

[0111] The humectant is hyaluronic acid; the emollient consists of 1:1 European hazelnut oil and jojoba seed oil; the preservative consists of 1:1.3 p-hydroxyacetophenone and 1,2-hexanediol by mass ratio;

[0112] Preparation method of essence oil 1:

[0113] S1: Mix deionized water, humectant and preservative uniformly to obtain an aqueous phase;

[0114] S2: Mix composition and emollient uniformly to obtain an oil phase;

[0115] S3: Add the oil phase to the aqueous phase to obtain a water-oil two-phase essence oil.

[0116] Essence oil 2:

[0117] Consists of the following mass percentage components:

[0118] Composition 1: 0.1wt%;

[0119] Humectant: 1wt%;

[0120] Emollient: 20wt%;

[0121] Preservative: 0.3 wt%;

[0122] Deionized water to 100 wt%;

[0123] The humectant is hyaluronic acid; the emollient consists of European hazelnut oil and jojoba seed oil in a mass ratio of 1:1; the preservative consists of p-hydroxyacetophenone and 1,2-hexanediol in a mass ratio of 1:1.3;

[0124] The preparation method is the same as that of the essential oil 1.

[0125] Essential oil 3:

[0126] Consists of the following mass percentage components:

[0127] Composition 1: 10 wt%;

[0128] Humectant: 1 wt%;

[0129] Emollient: 20 wt%;

[0130] Preservative: 0.3 wt%;

[0131] Deionized water to 100 wt%;

[0132] The humectant is hyaluronic acid; the emollient consists of European hazelnut oil and jojoba seed oil in a mass ratio of 1:1; the preservative consists of p-hydroxyacetophenone and 1,2-hexanediol in a mass ratio of 1:1.3;

[0133] The preparation method is the same as that of the essential oil 1.

[0134] Essential oil 4: Different from the essential oil 1, the composition 2 is used to replace the composition 1 in the same mass percentage, and the rest of the components and mass percentages, the preparation method and process parameters of the essential oil are the same as those of the essential oil 1.

[0135] Essential oil 5: Different from the essential oil 1, the composition 3 is used to replace the composition 1 in the same mass percentage, and the rest of the components and mass percentages, the preparation method and process parameters of the essential oil are the same as those of the essential oil 1.

[0136] Essential oil ①: Different from the essential oil 1, the composition ① is used to replace the composition 1 in the same mass percentage, and the rest of the components and mass percentages, the preparation method and process parameters of the essential oil are the same as those of the essential oil 1.

[0137] Essential oil ②: Different from the essential oil 1, the composition ② is used to replace the composition 1 in the same mass percentage, and the rest of the components and mass percentages, the preparation method and process parameters of the essential oil are the same as those of the essential oil 1.

[0138] Essence oil ③: Different from essence oil 1, composition ③ is used to replace composition 1 in the same mass percentage, and the preparation method and process parameters of the remaining components and mass percentages of the essence oil are the same as those of essence oil 1.

[0139] Essence oil ④: Different from essence oil 1, composition ④ is used to replace composition 1 in the same mass percentage, and the preparation method and process parameters of the remaining components and mass percentages of the essence oil are the same as those of essence oil 1.

[0140] Essence oil ⑤: Different from essence oil 1, composition ⑤ is used to replace composition 1 in the same mass percentage, and the preparation method and process parameters of the remaining components and mass percentages of the essence oil are the same as those of essence oil 1.

[0141] Essence oil ⑥: Different from essence oil 1, composition ⑥ is used to replace composition 1 in the same mass percentage, and the preparation method and process parameters of the remaining components and mass percentages of the essence oil are the same as those of essence oil 1.

[0142] Essence oil ⑦: Different from essence oil 1, composition ⑦ is used to replace composition 1 in the same mass percentage, and the preparation method and process parameters of the remaining components and mass percentages of the essence oil are the same as those of essence oil 1.

[0143] Essence oil ⑧: Different from essence oil 1, composition ⑧ is used to replace composition 1 in the same mass percentage, and the preparation method and process parameters of the remaining components and mass percentages of the essence oil are the same as those of essence oil 1.

[0144] Blank essence oil:

[0145] Composed of the following mass percentage components:

[0146] Moisturizer: 1wt%;

[0147] Emollient: 20wt%;

[0148] Preservative: 0.1wt%;

[0149] Deionized water to 100wt%;

[0150] Among them, the moisturizer is hyaluronic acid; the emollient is composed of European hazelnut oil and jojoba seed oil in a mass ratio of 1:1; the preservative is composed of p-hydroxyacetophenone and 1,2-hexanediol in a mass ratio of 1:1.3;

[0151] Preparation method of blank essence oil:

[0152] S1: Mix deionized water, moisturizer, and preservative uniformly to obtain an aqueous phase;

[0153] S2: Mix the emollient uniformly to obtain an oil phase;

[0154] S3: Add the oil phase to the water phase to obtain a two-phase water-oil essential oil.

[0155] Efficacy verification:

[0156] Experiment 1: Effect of the composition on type I collagen secretion;

[0157] Test samples: Compositions 1-3, ①-⑧;

[0158] The preparation method for the experimental group is as follows:

[0159] Compositions 1-3 and 1-8 were diluted separately using 50wt% ethanol-water solution and low-sugar DEME medium, and then tested; the mass ratio of 50wt% ethanol-water solution, composition, and low-sugar DEME medium was 2:1:97.

[0160] The preparation method is as follows: First, mix 50wt% ethanol-water solution with the composition evenly, then add low sugar DEME medium, mix evenly to obtain the test sample of the experimental group.

[0161] The preparation method for blank control test samples is as follows:

[0162] The mass ratio of 50wt% ethanol-water solution to low-sugar DEME medium is 2:98;

[0163] Preparation method: Mix 50wt% ethanol-water solution with low-sugar DEME medium to obtain blank test samples.

[0164] The positive group test sample was a 100 ng / mTGFβ1 working solution, which was prepared according to the preparation method described in Appendix B of the standard T / SHRH 031-2020 "Test of Firming and Anti-wrinkle Efficacy of Cosmetics - Determination of Type I Collagen Content in In Vitro Fibroblasts".

[0165] Experimental cells: human dermal fibroblasts.

[0166] The test method was conducted in accordance with the test method specified in standard T / SHRH 031-2020 "Test of Firming and Anti-wrinkle Efficacy of Cosmetics - Determination of Type I Collagen Content in In Vitro Fibroblasts".

[0167] Test kit: Human type I collagen (ColⅠ) ELISA test kit (COBIO Biotechnology, catalog number: CB10152-Hu).

[0168] Formula for calculating the upregulation rate of type I collagen:

[0169] Upward adjustment rate (%) = (T / C - 1) × 100%;

[0170] In the formula: T is the average value of the type I collagen content of the test sample; C is the average value of the type I collagen content of the blank group.

[0171] Table 1 Effect of the composition on the type I collagen content secreted by human dermal fibroblasts

[0172] Group Up-regulation rate (%) Composition 1 30.2 Composition 2 28.7 Composition 3 29.4 Composition 1 23.5 Composition 2 24.2 Composition 3 17.7 Composition 4 21.5 Composition 5 19.4 Composition 6 22.6 Composition 7 18.7 Composition 8 24.9 Positive group 39.5

[0173] According to the results in Table 1, the compositions 1-3 and the compositions ①-⑧ all have a certain effect of promoting the secretion of type I collagen; comparison of the results of the composition 1-3 and the composition ⑧ shows that, within the mass fraction range defined in the application, the effect of each component in the composition is better; comparison of the composition 1 and the compositions ①-② shows that, the PDRN from different raw materials has an influence on the effect of the composition in promoting the secretion of type I collagen, and the PDRN from the brown algae defined in the application has the best effect; comparison of the results of the composition 1 and the compositions ③-⑦ shows that, the PDRN, the extract of threeleaf falsestarwort, the lawn grass root oil, the rust red rose seed oil and the ginseng seed oil in the composition of the application have a significant synergistic effect, and when used together, they can significantly promote the secretion of type I collagen by human dermal fibroblasts.

[0174] Test 2: Safety test of the essence oil

[0175] The biological safety of the essence oils 1-5, the essence oils ①-⑧ and the blank essence oil is verified by tests in the application.

[0176] The irritation of the cosmetic is evaluated according to the 2015 Cosmetic Safety Technical Specification as the reference standard, the test method is the skin patch test, the test is performed on the subjects who are randomly distributed and positive in the lactic acid irritation test at the age of 16-65, and the subjects are randomly divided into groups, each group corresponds to one essence oil, and each group has 32 subjects.

[0177] Test object: the essence oils 1-5, the essence oils ①-⑧ and the blank essence oil.

[0178] Test method: before the test, the test object is mechanically emulsified, the emulsified test object is placed in the patch tester, the amount is 0.020-0.025g, the patch tester with the test object is covered with a non-irritating cloth-based adhesive tape on the back or the curved side of the forearm of the subject, and the skin surface is evenly attached by pressing with the palm for 24 hours, 30 minutes after removing the test patch tester, the skin reaction is observed after the indentation disappears. If the result is negative, the observation is performed again at 24 hours and 48 hours after the patch test.

[0179] The mechanical emulsification is to shake the test object vigorously to mix the sample water phase and oil phase to form an emulsion.

[0180] Evaluation criteria:

[0181] 0 grade: negative reaction;

[0182] 1 grade: suspicious reaction, only weak erythema;

[0183] 2 grade: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0184] 3 grade: strong positive reaction, erythema, infiltration, edema, and papules may be present, and the reaction may exceed the test area;

[0185] 4 grade: very strong positive reaction, obvious erythema, severe infiltration, edema, and confluent blisters, and the reaction exceeds the test area.

[0186] Test results: the skin reactions of all subjects were negative, indicating that the essential oil provided by the application is safe and suitable for sensitive skin groups.

[0187] Test 3: Effect of essential oil on skin tightening, anti-wrinkle, and barrier repair

[0188] Test samples: essential oil 1-5, essential oil 1-8, and blank essential oil; before use, the subjects should be informed to shake the water and oil phases in the test samples before use, and if necessary, the staff should demonstrate accordingly when distributing the test samples.

[0189] Subjects: sensitive skin subjects with positive lactic acid stinging at the age of 35-55, the subjects were grouped, each group corresponding to one test sample, and each group had 32 subjects.

[0190] Test period: skin elasticity determination, test period was 28 days; TEWL value determination test period was 14 days.

[0191] Test sample usage instructions: use the test sample on the test area twice a day, morning and evening, with a single dose of 2.00 mg / cm 2 .

[0192] The skin elasticity determination test area was the zygomatic part of the face, and the test area size was 3 cm x 3 cm; the TEWL value determination test area was the inner arm, and the test area size was 3 cm x 3 cm.

[0193] The TEWL value test method was determined according to the method described in the standard “T / ZHCA 003-2018 Cosmetics Influence Test Method for Trans-Epidermal Water Loss”.

[0194] The skin elasticity test method was determined according to the method described in the standard “T / ZHCA 005-2019 Cosmetics Influence Test Method for Skin Elasticity”.

[0195] TEWL value change rate (%) = (T0 (TEWL value) - T 14(T EWL value) / T0 (TEWL value) x 100%.

[0196] In the above formula, T0 (TEWL value) is the TEWL detection value on day 0; T 14 (T EWL value) is the TEWL detection value on day 14.

[0197] Skin elasticity change rate (%) = (T14 (TEWL value) - T0 (TEWL value)) / T0 (TEWL value) x 100%. .

[0198] In the above formula, 样品 is the average value of the difference in the elasticity representation value on the application side; is the average value of the difference in the elasticity representation value on the control side; wherein , The calculation method refers to the standard "T / ZHCA 005-2019 Cosmetics Influence on Skin Elasticity Test Method", wherein the application side is applied with the test sample, and the control side is applied with an equal amount of glycerol solution. The preparation method of the glycerol solution is described in the standard "T / ZHCA 005-2019 Cosmetics Influence on Skin Elasticity Test Method" 5.3.

[0199] When the above change rate is positive, it indicates that the represented characteristics have improved, and the higher the positive value, the better the improvement.

[0200] Table 2 Influence of essential oil on skin TEWL value and elasticity

[0201] Group Change rate of skin elasticity (%) Change rate of TEWL value (%) Essence oil 1 14.7 21.7 Essence oil 2 9.4 10.3 Essence oil 3 15.3 22.9 Essence oil 4 14.2 19.6 Essence oil 5 14.4 20.5 Essence oil 1 11.5 17.2 Essence oil 2 11.9 16.7 Essence oil 3 8.2 13.4 Essence oil 4 9.9 16.9 Essence oil 5 9.1 10.5 Essence oil 6 11.2 12.1 Essence oil 7 8.6 9.6 Essence oil 8 12.2 17.4 Blank essence oil 1.6 2.6

[0202] As can be seen from the results in Table 2, the essential oil provided by the present application has significant firming anti-wrinkle and skin barrier improvement effects; specifically, as can be seen from the results of the comparison of essential oils 1-5, 1-8 and the blank essential oil, the firming anti-wrinkle and skin barrier improvement effects of the essential oil are closely related to the composition; as can be seen from the results of the comparison of essential oils 1-3 and the blank essential oil, the addition amount of the composition has a certain influence on the firming anti-wrinkle and skin barrier improvement effects of the essential oil, and based on the consideration of cost performance, the present application selects essential oil 1 as the essential oil with the best effects; as can be seen from the results of the comparison of essential oil 1 and essential oils 4, 5 and 8, the mass fraction of each component in the composition has a certain influence on the firming anti-wrinkle and skin barrier improvement effects of the essential oil, and when the mass fraction of each component in the composition is within the range defined in the present application, the firming anti-wrinkle and skin barrier improvement effects of the essential oil obtained are better; as can be seen from the results of the comparison of essential oil 1 and essential oils 1-2, the PDRN from different raw materials has a certain influence on the firming anti-wrinkle and skin barrier improvement effects of the essential oil, and when the essential oil contains the PDRN from brown algae as defined in the present application, the firming anti-wrinkle and skin barrier improvement effects of the essential oil obtained are better; as can be seen from the results of the comparison of essential oil 1 and essential oils 3-6, the PDRN, the extract of threeleaf falsestarwort, the spodiopogon sibiricus root oil, the rust red rose seed oil and the panax ginseng seed oil in the composition have a significant synergistic effect, and when they are added to the essential oil as active substances, the firming anti-wrinkle and skin barrier improvement effects of the essential oil can be significantly improved.

[0203] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: they can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A composition containing PDRN with firming and anti-wrinkle effects, characterized in that, The composition comprises the following components in parts by weight: PDRN: 0.01-0.2 parts; Three-white grass extract: 0.1-0.3 parts; Vetiver root oil: 0.1-0.3 parts; Rusty Rosehip Seed Oil: 2-4 parts; Ginseng seed oil: 0.1-0.3 parts; The PDRN extraction raw material is brown algae.

2. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: PDRN: 0.1-0.2 parts; Three-white grass extract: 0.2-0.3 parts; Vetiver root oil: 0.2-0.3 parts; Rusty Rosehip Seed Oil: 3-4 parts; Ginseng seed oil: 0.2-0.3 parts.

3. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: PDRN: 0.1 part; Three-white grass extract: 0.2 parts; Vetiver root oil: 0.2 parts; Rusty Rosehip Seed Oil: 3 parts; Ginseng seed oil: 0.2 parts.

4. The use of the composition according to any one of claims 1-3 in the preparation of cosmetics with firming and anti-wrinkle effects.

5. The application as described in claim 4, characterized in that, The dosage form of the cosmetic is at least one of powder, emulsion, cream, and spray.

6. A two-phase water-oil essence oil with firming and anti-wrinkle effects, characterized in that, The essential oil comprises any one of the compositions containing PDRN and having firming and anti-wrinkle effects as described in claims 1-3.

7. The essential oil as described in claim 6, characterized in that, The essential oil also includes moisturizers, emollients, preservatives, and deionized water.

8. The essential oil as described in claim 7, characterized in that, The moisturizer includes at least one of glycerin, hyaluronic acid, and allantoin.

9. The essential oil as described in claim 7, characterized in that, The emollients include at least one of the following: European hazelnut seed oil, caprylic / capric triglyceride, triisocaprylic triglyceride, meadowfoam seed oil, C9-12 alkyl, jojoba seed oil, squalane, borage seed oil, and oat kernel oil.

10. The essential oil as described in claim 7, characterized in that, The preservative includes at least one of p-hydroxyacetophenone, 1,2-hexanediol, and parabens.