Tea leaf theanine composition for improving female menstrual syndrome and preparation method thereof

Abstract

The invention relates to the technical field of health-care food, and particularly discloses a tea leaf theanine composition for improving female menstrual syndromes and a preparation method thereof, and the tea leaf theanine composition comprises tea leaf theanine, traditional Chinese medicine powder for nourishing blood and regulating menstruation, gamma-aminobutyric acid, an anti-inflammatory berry extract, linseed oil, hawthorn fruit powder and a mixed fermentation product. The preparation method of the tea leaf theanine composition comprises the following steps: adding linseed oil into hawthorn fruit powder, uniformly stirring and mixing, adding tea leaf theanine, blood-nourishing menstruation-regulating traditional Chinese medicine powder, gamma-aminobutyric acid, an anti-inflammatory berry extract and a mixed fermentation product, dispersing at a high speed, freeze-drying, crushing and sieving to obtain the tea leaf theanine composition. The tea leaf theanine composition for improving the female menstrual syndrome is obtained. The tea leaf theanine composition prepared by the invention has a remarkable improvement effect on female menstrual syndromes, and the comprehensive effect is far better than that of each contrast. The improvement effect is stable and reliable, and the relieving effect on core symptoms such as dysmenorrhea, emotional fluctuation, fatigue and weakness is particularly outstanding.

Description

Technical Field

[0001] This application relates to the field of health food technology, and more specifically, it relates to a tea theanine composition for improving premenstrual syndrome in women and a method for preparing the same. Background Technology

[0002] Premenstrual syndrome (PMS) is a series of physiological and psychological syndromes that occur in women during specific phases of the menstrual cycle. Its incidence rate is as high as 40-70% among women of reproductive age, severely impacting their work efficiency, quality of life, and mental health. The clinical manifestations of this condition are diverse, with core symptoms including mood swings, physical discomfort, and digestive system abnormalities. Its pathogenesis is not fully understood, but it is currently believed to be closely related to neurotransmitter imbalances, endocrine disorders, and inflammatory activation caused by fluctuations in estrogen and progesterone levels.

[0003] Existing methods for improving premenstrual syndrome (PMS) are mainly divided into two categories: drug treatment and non-drug intervention. Drug treatment often uses Western medicines such as anti-anxiety drugs, non-steroidal anti-inflammatory drugs (NSAIDs), and hormone regulators. While these can quickly relieve some symptoms, long-term use can easily cause side effects such as gastrointestinal irritation, burden on liver and kidney function, and hormonal imbalances, and carries the risk of drug dependence, thus limiting their clinical application. Non-drug interventions include dietary adjustments, exercise therapy, psychological counseling, and supplementation with health products. Among these, health products are the first choice for women's daily conditioning due to their convenience and relatively high safety. Currently, most health products on the market for PMS are based on traditional Chinese medicine compound formulas and vitamin and mineral complexes, but they have many shortcomings: some traditional Chinese medicine compound formulas have complex ingredients, are either warm or cold in nature, limiting their applicable population, and their bitter taste leads to poor compliance; vitamin and mineral products mostly only supplement a single nutrient, with limited effects on improving core symptoms such as mood regulation and pain relief. Furthermore, there are few existing products with tea extract as the core active ingredient specifically formulated for conditioning, and they fail to fully utilize the synergistic effects of natural plant active ingredients. Theanine, a unique non-protein amino acid found in tea, is the core component responsible for tea's calming, anti-anxiety, and anti-inflammatory physiological activities. Modern pharmacological studies have confirmed that theanine can improve mood swings by regulating the levels of neurotransmitters such as γ-aminobutyric acid (GABA) and serotonin in the central nervous system; it can also inhibit the release of inflammatory factors, alleviating dysmenorrhea-related inflammatory responses, and is characterized by its non-toxicity and good biocompatibility. However, current applications of theanine are mostly focused on calming the nerves, aiding sleep, and combating fatigue, and a composition specifically targeting multiple symptoms of premenstrual syndrome (PMS) with theanine as the core and combined with other natural active ingredients has not yet been developed. Therefore, developing a composition with theanine as the core, combined with synergistic natural active ingredients, and with an efficient, safe, and easily industrialized preparation process to comprehensively improve multiple symptoms of PMS in women, while avoiding the shortcomings of existing drugs and health products, and meeting women's demand for natural, safe, and highly effective conditioning products, has significant clinical value and market prospects. Summary of the Invention

[0004] In order to solve the technical problems mentioned in the background art, this application provides a tea theanine composition for improving premenstrual syndrome in women and a method for preparing the same.

[0005] This application provides a tea-based theanine composition for improving premenstrual syndrome in women, employing the following technical solution: A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 15-25 parts tea theanine, 33-42 parts traditional Chinese medicine powder for nourishing blood and regulating menstruation, 5-8 parts γ-aminobutyric acid, 4-6 parts anti-inflammatory berry extract, 8-12 parts flaxseed oil, 6-10 parts hawthorn powder, and 3-5 parts mixed fermentation product.

[0006] Preferably, the blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of Angelica sinensis powder and Paeonia lactiflora powder in a mass ratio of 10-22:15-25.

[0007] Preferably, the anti-inflammatory berry extract is cranberry extract.

[0008] Preferably, the method for preparing the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose powder and mulberry powder to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4-6℃ for 12-16 hours, then centrifuge and freeze dry to obtain the fermentation substrate; (3) Add privet extract and sterile water to the fermentation substrate, stir and mix at 30-35℃ for 20-30 minutes, and sterilize by moist heat sterilization to obtain fermentation broth; inoculate fermentation bacteria into fermentation broth, and after fermentation, obtain mixed fermentation product.

[0009] Preferably, the mass ratio of wolfberry powder, rose powder and mulberry powder in step (1) is 20-30:5-8:4-10.

[0010] Preferably, the mass ratio of the mixture, vitamin E and anhydrous ethanol in step (2) is 10-15:0.1-0.5:40-60.

[0011] Preferably, in step (3), the mass ratio of fermentation substrate, privet extract and sterile water is 10-12:2-4:40-50.

[0012] Preferably, the amount of fermentation bacteria in step (3) is 3-5% of the mass of the fermentation broth.

[0013] Preferably, the fermentation bacteria in step (3) consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective viable count ratio of 10-12:4-8.

[0014] Preferably, the fermentation temperature in step (3) is 35-37℃, and the anaerobic fermentation time is 24-48h.

[0015] This application also provides a method for preparing a tea theanine composition that improves premenstrual syndrome in women, using the following technical solution: A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Flaxseed oil was added to hawthorn powder and stirred until well mixed. Then, tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products were added. The mixture was placed in a high-speed disperser and stirred and dispersed. After freeze-drying, it was pulverized and sieved to obtain a tea theanine composition for improving women's premenstrual syndrome.

[0016] In summary, this application has the following beneficial effects: This application establishes its core conditioning foundation through the classic combination of Angelica sinensis and Paeonia lactiflora, known for their blood-nourishing and menstrual-regulating properties. In Traditional Chinese Medicine, Angelica sinensis is known for its ability to replenish and invigorate blood, promote blood circulation, and relieve pain, while Paeonia lactiflora is known for its ability to nourish blood, soothe the liver, and alleviate pain. When combined in a scientific ratio, these two ingredients can precisely target core issues such as dysmenorrhea and irregular menstruation caused by blood deficiency and qi stagnation during menstruation, forming a synergistic conditioning loop that nourishes blood and relieves pain. The application also incorporates the dual mood-soothing effects of theanine and γ-aminobutyric acid (GABA) from tea. Theanine can alleviate anxiety and irritability by regulating central neurotransmitters, while GABA can directly inhibit nerve excitation and stabilize mood fluctuations. The two complement each other, enhancing the intervention effect on mood-related symptoms. Furthermore, the application combines the anti-inflammatory activity of cranberry extract, rich in anthocyanins and proanthocyanidins, which can inhibit the release of inflammatory factors and alleviate inflammation-related symptoms such as breast tenderness and pelvic congestion during menstruation. Finally, the application integrates the Omega-3 fatty acids from flaxseed oil and the organic acid components from hawthorn powder. The former lubricates the intestines, while the latter aids digestion and eliminates food stagnation, working together to harmonize gastrointestinal function during menstruation and improve physical discomfort such as nausea, vomiting, and bloating. Simultaneously, an innovative mixed fermentation preparation process was designed, using wolfberry powder (for nourishing the liver and kidneys), rose powder (for regulating qi and relieving depression), and mulberry powder (for nourishing yin and replenishing blood) as raw materials. Targeted extraction was achieved by precisely controlling the ethanol concentration, extraction temperature, and solid-liquid ratio to maximize the enrichment of active ingredients. The liver and kidney nourishing effects of privet fruit extract were then added, along with the synergistic fermentation of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus. The former regulates the intestinal microecology, while the latter enhances the immune barrier, forming a multi-target synergistic mechanism of blood-nourishing, soothing, and anti-inflammatory microecological regulation. This results in a composition that significantly alleviates pain symptoms such as dysmenorrhea and breast tenderness, as well as mood symptoms such as irritability and emotional fluctuations. Detailed Implementation

[0017] The present application will be further described in detail below with reference to the embodiments.

[0018] The tea theanine, wolfberry powder, rose pollen, and mulberry powder used in the embodiments and comparative examples of this application were all provided by Hunan Jinong Bio-resources Co., Ltd.; Angelica sinensis powder (food grade) was purchased from Guangzhou Huayu Biotechnology Co., Ltd.; Paeonia lactiflora powder (food grade) was purchased from Wuhan Maikairui Chemical Co., Ltd.; cranberry extract (food grade) was purchased from Guangdong Mingcheng Biotechnology Co., Ltd.; γ-aminobutyric acid was purchased from Jiangsu Enming Bioengineering Technology Co., Ltd.; flaxseed oil was purchased from Guyuan Biotechnology (Tianjin) Co., Ltd.; hawthorn powder was purchased from Hebei Tuohai Biotechnology Co., Ltd.; privet fruit extract was purchased from Shaanxi Junhe Biotechnology Co., Ltd.; Bifidobacterium animalis subsp. lactis BI516 and Lactobacillus acidophilus LA11-Onlly were both provided by Shanghai Jiaotong University Angli Co., Ltd., with strain preservation numbers CGMCC 11959 and CGMCC 2106, respectively.

[0019] Examples 1-3 provide a tea theanine composition for improving premenstrual syndrome in women and its preparation method.

[0020] Example 1 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 15 parts tea theanine, 33 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 5 parts γ-aminobutyric acid, 4 parts anti-inflammatory berry extract, 8 parts flaxseed oil, 6 parts hawthorn powder, and 3 parts mixed fermentation product. The blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of angelica powder and white peony powder in a mass ratio of 10:15, and the anti-inflammatory berry extract is cranberry extract. The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 20:5:4 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4°C for 12 h to settle, then centrifuge at 2000 rpm for 10 min and freeze dry at -20°C for 4 h to obtain the fermentation substrate. Control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 10:0.1:40. (3) Add privet extract and sterile water to the fermentation substrate, stir and mix for 20 min at 30℃ and 200 rpm, sterilize by moist heat sterilization for 15 min at 103.4 kPa and 121℃ to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at 3% of the mass of fermentation broth, ferment at 35℃, ferment for 24 h, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate, privet extract and sterile water to be 10:2:40. The fermentation bacteria consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective viable count ratio of 10:4.

[0021] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Add flaxseed oil to hawthorn powder and stir at 200 rpm until well mixed. Then add tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products. Place in a high-speed disperser and stir at 1000 rpm to disperse. Freeze-dry at -20℃ for 10 hours and pulverize through a 40-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0022] Example 2 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 20 parts tea theanine, 38 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 7 parts γ-aminobutyric acid, 5 parts anti-inflammatory berry extract, 10 parts flaxseed oil, 8 parts hawthorn powder, and 4 parts mixed fermentation product. The blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of angelica powder and white peony powder in a mass ratio of 16:20, and the anti-inflammatory berry extract is cranberry extract. The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 25:7:8 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 5°C for 14 h to settle, then centrifuge at 3000 rpm for 12 min and freeze dry at -30°C for 6 h to obtain the fermentation substrate. Control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 12:0.3:50. (3) Add privet extract and sterile water to the fermentation substrate, stir and mix at 300 rpm for 25 min at 32℃, sterilize by wet heat sterilization at 103.4 kPa and 121℃ for 22 min to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at an inoculation amount of 4% of the mass of fermentation broth, ferment at 36℃, ferment for 36 h, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate, privet extract and sterile water to be 11:3:45. The fermentation bacteria consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective viable count ratio of 11:6.

[0023] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Flaxseed oil was added to hawthorn powder and stirred at 250 rpm until well mixed. Then, tea theanine, blood-nourishing and menstrual-regulating Chinese herbal powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products were added. The mixture was placed in a high-speed disperser and stirred at 1500 rpm until dispersed. The mixture was then freeze-dried at -30℃ for 11 hours and pulverized through a 50-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0024] Example 3 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 25 parts tea theanine, 42 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 8 parts γ-aminobutyric acid, 6 parts anti-inflammatory berry extract, 12 parts flaxseed oil, 10 parts hawthorn powder, and 5 parts mixed fermentation product. The blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of angelica powder and white peony powder in a mass ratio of 22:25, and the anti-inflammatory berry extract is cranberry extract. The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 30:8:10 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 6°C for 16 h, centrifuge at 4000 rpm for 15 min, freeze dry at -40°C for 8 h to obtain the fermentation substrate, and control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 15:0.5:60. (3) Add privet extract and sterile water to the fermentation substrate, stir and mix at 35°C and 400 rpm for 30 min, sterilize by moist heat sterilization at 103.4 kPa and 121°C for 30 min to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at 5% of the mass of fermentation broth, ferment at 37°C for 48 h, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate, privet extract and sterile water to 12:4:50. The fermentation bacteria consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective viable count ratio of 12:8.

[0025] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Flaxseed oil was added to hawthorn powder and stirred at 300 rpm until evenly mixed. Then, tea theanine, blood-nourishing and menstrual-regulating Chinese herbal powder, γ-aminobutyric acid, anti-inflammatory berry extract, and mixed fermentation products were added. The mixture was placed in a high-speed disperser and stirred at 2000 rpm until dispersed. The mixture was then freeze-dried at -40℃ for 12 hours and pulverized through a 60-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0026] Comparative Example 1 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 15 parts tea theanine, 33 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 5 parts γ-aminobutyric acid, 4 parts anti-inflammatory berry extract, 8 parts flaxseed oil, 6 parts hawthorn powder, and 3 parts mixed fermentation product, wherein the blood-nourishing and menstruation-regulating traditional Chinese medicine powder is angelica powder, and the anti-inflammatory berry extract is cranberry extract. The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 20:5:4 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4°C for 12 h to settle, then centrifuge at 2000 rpm for 10 min and freeze dry at -20°C for 4 h to obtain the fermentation substrate. Control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 10:0.1:40. (3) Add privet extract and sterile water to the fermentation substrate, stir and mix for 20 min at 30℃ and 200 rpm, sterilize by moist heat sterilization for 15 min at 103.4 kPa and 121℃ to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at 3% of the mass of fermentation broth, ferment at 35℃, ferment for 24 h, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate, privet extract and sterile water to be 10:2:40. The fermentation bacteria consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective viable count ratio of 10:4.

[0027] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Add flaxseed oil to hawthorn powder and stir at 200 rpm until well mixed. Then add tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products. Place in a high-speed disperser and stir at 1000 rpm to disperse. Freeze-dry at -20℃ for 10 hours and pulverize through a 40-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0028] Comparative Example 2 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 15 parts tea theanine, 33 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 5 parts γ-aminobutyric acid, 4 parts anti-inflammatory berry extract, 8 parts flaxseed oil, 6 parts hawthorn powder, and 3 parts mixed fermentation product, wherein the blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of white peony powder, and the anti-inflammatory berry extract is cranberry extract; The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 20:5:4 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4°C for 12 h to settle, then centrifuge at 2000 rpm for 10 min and freeze dry at -20°C for 4 h to obtain the fermentation substrate. Control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 10:0.1:40. (3) Add privet extract and sterile water to the fermentation substrate, stir and mix for 20 min at 30℃ and 200 rpm, sterilize by moist heat sterilization for 15 min at 103.4 kPa and 121℃ to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at 3% of the mass of fermentation broth, ferment at 35℃, ferment for 24 h, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate, privet extract and sterile water to be 10:2:40. The fermentation bacteria consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective viable count ratio of 10:4.

[0029] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Add flaxseed oil to hawthorn powder and stir at 200 rpm until well mixed. Then add tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products. Place in a high-speed disperser and stir at 1000 rpm to disperse. Freeze-dry at -20℃ for 10 hours and pulverize through a 40-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0030] Comparative Example 3 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 15 parts tea theanine, 33 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 5 parts γ-aminobutyric acid, 4 parts anti-inflammatory berry extract, 8 parts flaxseed oil, 6 parts hawthorn powder, and 3 parts mixed fermentation product. The blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of angelica powder and white peony powder in a mass ratio of 10:15, and the anti-inflammatory berry extract is cranberry extract. The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 20:5:4 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4°C for 12 h to settle, then centrifuge at 2000 rpm for 10 min and freeze dry at -20°C for 4 h to obtain the fermentation substrate. Control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 10:0.1:40. (3) Add sterile water to the fermentation substrate, stir and mix for 20 minutes at 30°C and 200 rpm, sterilize by wet heat sterilization for 15 minutes at 103.4 kPa and 121°C to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at 3% of the mass of fermentation broth, ferment at 35°C, ferment for 24 hours, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate to sterile water to be 10:40. The fermentation bacteria consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective viable count ratio of 10:4.

[0031] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Add flaxseed oil to hawthorn powder and stir at 200 rpm until well mixed. Then add tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products. Place in a high-speed disperser and stir at 1000 rpm to disperse. Freeze-dry at -20℃ for 10 hours and pulverize through a 40-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0032] Comparative Example 4 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 15 parts tea theanine, 33 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 5 parts γ-aminobutyric acid, 4 parts anti-inflammatory berry extract, 8 parts flaxseed oil, 6 parts hawthorn powder, and 3 parts mixed fermentation product. The blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of angelica powder and white peony powder in a mass ratio of 10:15, and the anti-inflammatory berry extract is cranberry extract. The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 20:5:4 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4°C for 12 h to settle, then centrifuge at 2000 rpm for 10 min and freeze dry at -20°C for 4 h to obtain the fermentation substrate. Control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 10:0.1:40. (3) Add privet extract and sterile water to the fermentation substrate, stir and mix for 20 min at 30℃ and 200 rpm, sterilize by moist heat sterilization for 15 min at 103.4 kPa and 121℃ to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at 3% of the mass of fermentation broth, ferment at 35℃, ferment for 24 h, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate, privet extract and sterile water to be 10:2:40. The fermentation bacteria is Lactobacillus acidophilus.

[0033] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Add flaxseed oil to hawthorn powder and stir at 200 rpm until well mixed. Then add tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products. Place in a high-speed disperser and stir at 1000 rpm to disperse. Freeze-dry at -20℃ for 10 hours and pulverize through a 40-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0034] Comparative Example 5 A tea-based theanine composition for improving premenstrual syndrome in women comprises the following components in parts by weight: 15 parts tea theanine, 33 parts blood-nourishing and menstruation-regulating traditional Chinese medicine powder, 5 parts γ-aminobutyric acid, 4 parts anti-inflammatory berry extract, 8 parts flaxseed oil, 6 parts hawthorn powder, and 3 parts mixed fermentation product, wherein the blood-nourishing and menstruation-regulating traditional Chinese medicine powder is composed of angelica powder and white peony powder in a mass ratio of 10:15, and the anti-inflammatory berry extract is cranberry extract; The preparation method of the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose pollen and mulberry powder in a mass ratio of 20:5:4 to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4°C for 12 h to settle, then centrifuge at 2000 rpm for 10 min and freeze dry at -20°C for 4 h to obtain the fermentation substrate. Control the mass ratio of the mixture, vitamin E and anhydrous ethanol to be 10:0.1:40. (3) Add privet extract and sterile water to the fermentation substrate, stir and mix for 20 min at 30℃ and 200 rpm, sterilize by moist heat sterilization for 15 min at 103.4 kPa and 121℃ to obtain fermentation broth; inoculate fermentation bacteria into the fermentation broth at 3% of the mass of fermentation broth, ferment at 35℃, ferment for 24 h, and after fermentation, obtain mixed fermentation product. Control the mass ratio of fermentation substrate, privet extract and sterile water to be 10:2:40. The fermentation bacteria are Bifidobacterium lactis subsp. animalis.

[0035] A method for preparing a tea theanine composition for improving premenstrual syndrome in women includes the following preparation steps: Add flaxseed oil to hawthorn powder and stir at 200 rpm until well mixed. Then add tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products. Place in a high-speed disperser and stir at 1000 rpm to disperse. Freeze-dry at -20℃ for 10 hours and pulverize through a 40-mesh sieve to obtain a tea theanine composition for improving premenstrual syndrome in women.

[0036] Performance testing (a) Subject design Women aged 18-45 who meet the clinical diagnostic criteria for premenstrual syndrome (PMS), have experienced clear PMS symptoms (dysmenorrhea, mood swings, etc.) in the past three menstrual cycles, have no history of severe liver or kidney dysfunction, endocrine disorders, or allergies, and voluntarily sign an informed consent form. Excluded individuals include pregnant or breastfeeding women, patients with gynecological organic diseases, those currently taking endocrine-affecting or analgesic medications, and those allergic to any component of the composition.

[0037] A randomized controlled trial was conducted, with experimental groups (Examples 1-3) and control groups (Comparative Examples 1-5), each with 30 participants. There were no significant differences in age or disease duration between the groups (P > 0.05).

[0038] (II) Dosing regimen Take the recommended daily dose for adults (5g once daily) dissolved in warm water for three consecutive menstrual cycles. During the treatment period: Subjects should avoid spicy, stimulating, and cold foods, avoid strenuous exercise, maintain a regular sleep schedule, and refrain from taking other health supplements or medications that improve PMS.

[0039] (III) Test Period The total cycle was 3 menstrual cycles. Indicators were measured and recorded before medication (baseline), after the end of the first menstrual cycle, and after the end of the third menstrual cycle.

[0040] Clinical efficacy evaluation symptom scoring test: The Menstrual Syndrome Symptom Rating Scale was used to quantify the core symptoms (0-4 points: 0 points - no symptoms, 1 point - mild, 2 points - moderate, 3 points - severe, 4 points - severely affecting daily life). Specific indicators are as follows: Pain: dysmenorrhea, breast tenderness, headache; Emotional: irritability, anxiety, depression, mood swings; Physical: fatigue, nausea and vomiting, abdominal distension, edema. The scores of each symptom and the total score were calculated, and the differences before and after medication and between groups were compared.

[0041] Calculation of improvement rate and effectiveness: Symptom improvement rate = (Pre-drug score - Post-drug score) / Pre-drug score × 100%; Total effective rate is determined as follows: markedly effective (total score improvement rate ≥ 70%), effective (30% ≤ total score improvement rate < 70%), and ineffective (total score improvement rate < 30%). Total effective rate = (number of markedly effective cases + number of effective cases) / total number of cases × 100%; The test results are shown in Table 1.

[0042] Table 1 Performance parameters of tea-theanine composition in improving premenstrual syndrome in women

[0043] As shown in Table 1, the tea-theanine composition prepared in this application has a significant effect on improving premenstrual syndrome (PMS) in women, and its overall effect far exceeds that of the individual comparison ratios. Its improvement effect is stable and reliable, with particularly outstanding relief of core symptoms such as dysmenorrhea, mood swings, and fatigue.

[0044] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A tea-based theanine composition for improving premenstrual syndrome in women, characterized in that, It includes the following components by weight: 15-25 parts of tea theanine, 33-42 parts of traditional Chinese medicine powder for nourishing blood and regulating menstruation, 5-8 parts of γ-aminobutyric acid, 4-6 parts of anti-inflammatory berry extract, 8-12 parts of flaxseed oil, 6-10 parts of hawthorn powder, and 3-5 parts of mixed fermentation products.

2. The tea theanine composition for improving premenstrual syndrome in women according to claim 1, characterized in that, The blood-nourishing and menstruation-regulating Chinese herbal powder is composed of Angelica sinensis powder and Paeonia lactiflora powder in a mass ratio of 10-22:15-25.

3. The tea theanine composition for improving premenstrual syndrome in women according to claim 1, characterized in that, The anti-inflammatory berry extract is cranberry extract.

4. The tea theanine composition for improving premenstrual syndrome in women according to claim 1, characterized in that, The method for preparing the mixed fermentation product includes the following preparation steps: (1) Mix wolfberry powder, rose powder and mulberry powder to obtain a mixture; (2) Add vitamin E and anhydrous ethanol to the mixture, let it stand at 4-6℃ for 12-16 hours, then centrifuge and freeze dry to obtain the fermentation substrate; (3) Add privet extract and sterile water to the fermentation substrate, stir and mix at 30-35℃ for 20-30 minutes, and sterilize by moist heat sterilization to obtain fermentation broth; inoculate fermentation bacteria into fermentation broth, and after fermentation, obtain mixed fermentation product.

5. The tea theanine composition for improving premenstrual syndrome in women according to claim 4, characterized in that, In step (3), the mass ratio of fermentation substrate, privet extract and sterile water is 10-12:2-4:40-50.

6. The tea theanine composition for improving premenstrual syndrome according to claim 4, characterized in that, In step (3), the amount of fermentation bacteria inoculated is 3-5% of the mass of the fermentation liquid.

7. The tea theanine composition for improving premenstrual syndrome in women according to claim 4, characterized in that, In step (3), the fermentation bacteria consist of Bifidobacterium animalis subsp. lactis and Lactobacillus acidophilus with an effective live bacteria ratio of 10-12:4-8.

8. The tea theanine composition for improving premenstrual syndrome in women according to claim 4, characterized in that, In step (3), the fermentation temperature is 35-37℃, and the anaerobic fermentation time is 24-48h.

9. A method for preparing a tea theanine composition for improving premenstrual syndrome as described in any one of claims 1-8, characterized in that, The preparation steps include the following: Flaxseed oil was added to hawthorn powder and stirred until well mixed. Then, tea theanine, blood-nourishing and menstrual-regulating Chinese medicine powder, γ-aminobutyric acid, anti-inflammatory berry extract and mixed fermentation products were added. The mixture was placed in a high-speed disperser and stirred and dispersed. After freeze-drying, it was pulverized and sieved to obtain a tea theanine composition for improving women's premenstrual syndrome.

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