Preparation method of organic compound and application of organic compound in soil

Abstract

The invention belongs to the technical field of fertilizers, and particularly relates to a preparation method of an organic compound and application of the organic compound in soil. The organic compound is prepared from the following raw materials: humic acid, maltodextrin, a microbial agent, polyacrylamide, oat bran, cow dung and brown algae. The microbial agent comprises nitrifying bacteria, rhizobium radiobium, bacillus licheniformis and bacillus megatherium. According to the invention, the raw materials are compounded for use, and the raw materials have a synergistic effect, so that the efficient organic compound capable of improving the soil fertility is provided. The raw materials of the organic compound are easy to obtain, the rice ear number is remarkably increased, the rice seed number per ear is increased, the rice dry grain weight is increased, the rice yield is effectively increased, the potato yield is effectively increased, and good economic benefits and social benefits are achieved.

Description

Technical Field

[0001] This invention belongs to the field of fertilizer technology, specifically relating to a method for preparing an organic compound and its application in soil. Background Technology

[0002] Organic fertilizers are fertilizers containing organic matter that provide crops with a variety of inorganic and organic nutrients while also improving soil fertility. High in organic matter, organic fertilizers improve soil structure, enhance its physical and chemical properties, increase its water and fertilizer retention capacity, and alleviate soil compaction caused by long-term use of chemical fertilizers, thus effectively achieving stable and increased yields and promoting healthy diets.

[0003] While organic fertilizers play a vital role in improving soil fertility and crop yield and quality, improper application methods can lead to a series of problems. Long-term application of organic compounds alone can reduce available phosphorus in black soil. Experiments have shown that applying organic fertilizer alone is detrimental to high and stable rice yields. After transplanting late-season rice, the soil in the organic fertilizer-treated plots showed severe foaming, dark black roots, significantly poor development, and significantly delayed seedling recovery. Excessive application of manure, especially pig manure, can cause the accumulation of salts and heavy metals in the soil. Studies have shown that the application of organic fertilizers can lead to the accumulation of nitrates in the soil.

[0004] Research on organic fertilizers should continue to be conducted in conjunction with different regional environments, climates, planting systems, and soil conditions to study their role in nutrient transformation, cycling, and balance in the crop-soil ecosystem, as well as nutrient balance in the soil-plant-animal food chain, so as to fully utilize soil nutrient resources and organic compounds to improve fertilizer utilization.

[0005] Chinese patent CN119735479A discloses an organic fertilizer made from livestock waste and its preparation method. The organic fertilizer includes livestock waste nutrient base, soil amendment particles, hexadecyl alcohol ester, calcium disodium ethylenediaminetetraacetate, and hydrolyzed polymaleic anhydride. The raw materials used are relatively expensive.

[0006] Different organic materials have varying effects on promoting soil fertility. Existing organic fertilizers have relatively low nutrient content and their nutrient utilization rate is not ideal. Therefore, further research into improving the types and preparation methods of organic compounds, increasing the nutrient content in organic compounds, and improving crop yield are urgent practical needs. Summary of the Invention

[0007] The purpose of this invention is to provide a method for preparing an organic compound and its application in soil. The organic compound of this invention uses readily available raw materials and significantly increases the number of rice panicles, the number of filled grains per panicle, and the dry grain weight of rice, effectively improving crop yield and demonstrating good economic and social benefits.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] The first objective of this invention is to provide an organic compound.

[0010] A second objective of this invention is to provide a method for preparing the above-mentioned organic complex.

[0011] A third objective of this invention is to provide the application of the above-mentioned organic compound in improving soil fertility.

[0012] To achieve the first objective, the technical solution adopted by this invention is as follows:

[0013] The present invention provides an organic complex made of humic acid, maltodextrin, microbial inoculants, polyacrylamide, oat bran, cow dung and brown algae.

[0014] In some embodiments, the organic complex is made from the following raw materials in parts by weight: 10-20 parts humic acid, 6-12 parts maltodextrin, 6-12 parts microbial inoculant, 3-8 parts polyacrylamide, 10-20 parts oat bran, 3-8 parts cow dung, and 6-12 parts brown algae.

[0015] In some preferred embodiments, the organic complex is made from the following raw materials in parts by weight: 15 parts humic acid, 9 parts maltodextrin, 9 parts microbial inoculant, 6 parts polyacrylamide, 15 parts oat bran, 6 parts cow dung, and 9 parts brown algae.

[0016] In some embodiments, the microbial agent comprises nitrifying bacteria, rhizobium radiation, Bacillus licheniformis, and Bacillus megaterium with a live bacteria ratio of 1:1.0-2.5:0.8-1.5:0.5-1.3.

[0017] In some preferred embodiments, the ratio of viable counts of the nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium is 1:2:1:0.8.

[0018] To achieve the second objective, the technical solution adopted by this invention is as follows:

[0019] The preparation method of the organic compound of the present invention is as follows: (1) air-dry cow dung and crush it; (2) mix microbial inoculants evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial inoculants to obtain the organic compound of the present invention.

[0020] To achieve the third objective, the technical solution adopted by this invention is as follows:

[0021] This invention provides the use of the organic compound in improving soil fertility.

[0022] In some embodiments, the organic compound increases the number of panicles in rice.

[0023] In some embodiments, the organic compound increases the number of grains per panicle of rice.

[0024] In some embodiments, the organic compound increases the dry grain weight of rice.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The polyacrylamide in the organic compound of this invention can improve soil structure, increase soil porosity and aeration, enhance soil water retention capacity, and reduce soil permeability, thereby improving soil fertility and crop yield.

[0027] This invention combines humic acid, maltodextrin, microbial agents, polyacrylamide, oat bran, cow manure, and brown algae, among other raw materials, to provide a highly efficient organic compound that enhances soil fertility.

[0028] The organic compound of this invention uses readily available raw materials, significantly increases the number of rice panicles, the number of grains per panicle, and the dry grain weight of rice, effectively improving rice yield and potato yield, and has good economic and social benefits. Attached Figure Description

[0029] Figure 1 The comparison of rice yield under different treatments shows that the different lowercase letters above the bars indicate significant differences between different groups (P < 0.05).

[0030] Figure 2 Comparison of potato tuber yield under different treatments: Different lowercase letters above the bars indicate significant differences between different groups (P < 0.05).

[0031] Figure 3 Comparison of potato yields under different treatments: Different lowercase letters above the bars indicate significant differences between different groups (P < 0.05). Detailed Implementation

[0032] The embodiments of the present invention will be described in detail below with reference to examples. However, those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be regarded as limiting the scope of the present invention.

[0033] Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the invention.

[0034] Example 1 An organic complex, the components and preparation method are as follows:

[0035] 15 parts humic acid, 9 parts maltodextrin, 9 parts microbial inoculant, 6 parts polyacrylamide, 15 parts oat bran, 6 parts cow dung, and 9 parts brown algae.

[0036] The ratio of viable counts of nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium in the microbial agent is 1:2:1:0.8, with a total viable count of 3.0 × 10⁻⁶. 9 cfu / g.

[0037] The preparation method is as follows: (1) air-dry cow dung and crush it; (2) mix microbial agents evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial agents to obtain the organic complex of the present invention.

[0038] Example 2 An organic complex, the components and preparation method are as follows:

[0039] 20 parts humic acid, 6 parts maltodextrin, 12 parts microbial inoculant, 3 parts polyacrylamide, 10 parts oat bran, 3 parts cow dung, and 12 parts brown algae.

[0040] The ratio of viable bacteria (nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium) in the microbial inoculant is 1:1:1:1, with a total viable count of 3.2 × 10⁻⁶. 9 cfu / g.

[0041] The preparation method is as follows: (1) air-dry cow dung and crush it; (2) mix microbial agents evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial agents to obtain the organic complex of the present invention.

[0042] Example 3 An organic complex, the components and preparation method are as follows:

[0043] 10 parts humic acid, 12 parts maltodextrin, 6 parts microbial inoculant, 8 parts polyacrylamide, 20 parts oat bran, 8 parts cow dung, and 6 parts brown algae.

[0044] The ratio of viable bacteria (nitrifying bacteria, Rhizobium radiationis, Bacillus licheniformis, and Bacillus megaterium) in the microbial agent is 1:2.5:0.8:1.3, with a total viable count of 2.5 × 10⁻⁶. 9 cfu / g.

[0045] The preparation method is as follows: (1) air-dry cow dung and crush it; (2) mix microbial agents evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial agents to obtain the organic complex of the present invention.

[0046] Example 4 An organic complex, the components and preparation method are as follows:

[0047] 15 parts humic acid, 12 parts maltodextrin, 6 parts microbial inoculant, 6 parts polyacrylamide, 20 parts oat bran, 6 parts cow dung, and 10 parts brown algae.

[0048] The ratio of viable counts of nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium in the microbial agent is 1:1:1.5:1.3, with a total viable count of 2.8 × 10⁻⁶. 9 cfu / g.

[0049] The preparation method is as follows: (1) air-dry cow dung and crush it; (2) mix microbial agents evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial agents to obtain the organic complex of the present invention.

[0050] Example 5 An organic complex, the components and preparation method are as follows:

[0051] 10 parts humic acid, 10 parts maltodextrin, 6 parts microbial inoculant, 8 parts polyacrylamide, 15 parts oat bran, 8 parts cow dung, and 9 parts brown algae.

[0052] The ratio of viable counts of nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium in the microbial agent is 1:2:1:0.8, with a total viable count of 3.0 × 10⁻⁶. 9 cfu / g.

[0053] The preparation method is as follows: (1) air-dry cow dung and crush it; (2) mix microbial agents evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial agents to obtain the organic complex of the present invention.

[0054] Example 6

[0055] 10 parts humic acid, 12 parts maltodextrin, 6 parts microbial inoculant, 8 parts polyacrylamide, 20 parts oat bran, 8 parts cow dung, and 6 parts brown algae.

[0056] The ratio of viable bacteria (nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium) in the microbial agent is 1:2.5:0.8:1.3, with a total viable count of 3.2 × 10⁻⁶. 9 cfu / g.

[0057] The preparation method is as follows: (1) air-dry cow dung and crush it; (2) mix microbial agents evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial agents to obtain the organic complex of the present invention.

[0058] Example 7 An organic complex, the components and preparation method are as follows:

[0059] 10 parts humic acid, 10 parts maltodextrin, 6 parts microbial inoculant, 8 parts polyacrylamide, 15 parts oat bran, 8 parts cow dung, and 9 parts brown algae.

[0060] The ratio of viable bacteria (nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium) in the microbial agent is 1:1:1:1, with a total viable count of 2.5 × 10⁻⁶. 9 cfu / g.

[0061] The preparation method is as follows: Weigh each raw medicinal material, crush it, soak it in water for 1.5 hours, decoct it twice, each time adding water at a rate of 5 times the total amount of medicinal materials, and decoct for 0.5 hours each time, and combine the decoctions. The preparation method is as follows: (1) Air dry cow dung naturally and crush it; (2) Mix the microbial agent evenly; (3) Mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix it with cow dung and microbial agent to obtain the organic complex of the present invention.

[0062] Comparative Example 1: An organic complex, the components and preparation method are as follows:

[0063] Compared with Example 1, the only difference is that the microbial agent is nitrifying bacteria, rhizobium radiation, and Bacillus megaterium, wherein the ratio of viable counts of nitrifying bacteria, rhizobium radiation, and Bacillus megaterium is 1:2:0.8, and the total viable count is 3.0 × 10⁻⁶. 9 cfu / g.

[0064] The preparation method is the same as in Example 1.

[0065] Comparative Example 2: An organic complex, the components and preparation method are as follows:

[0066] Compared with Example 1, the only difference is that the microbial agent is nitrifying bacteria, Bacillus licheniformis, and Bacillus megaterium, wherein the ratio of viable counts of nitrifying bacteria, Bacillus licheniformis, and Bacillus megaterium is 1:1:0.8, and the total viable count is 3.0 × 10⁻⁶. 9 cfu / g.

[0067] The preparation method is the same as in Example 1.

[0068] Comparative Example 3: An organic complex, the components and preparation method are as follows:

[0069] Compared with Example 1, the only difference is that the microbial agent is nitrifying bacteria and Bacillus megaterium, wherein the ratio of viable bacteria to viable bacteria of nitrifying bacteria is 1:0.8, and the total viable bacteria count is 3.0 × 10⁻⁶. 9 cfu / g.

[0070] The preparation method is the same as in Example 1.

[0071] Comparative Example 4: An organic complex, the components and preparation method are as follows:

[0072] 9 parts maltodextrin, 12 parts microbial inoculant, 8 parts polyacrylamide, 20 parts oat bran, 8 parts cow dung, and 12 parts brown algae.

[0073] The ratio of viable counts of nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium in the microbial agent is 1:2:1:0.8, with a total viable count of 3.0 × 10⁻⁶. 9 cfu / g.

[0074] The preparation method is the same as in Example 1.

[0075] Comparative Example 5: An organic complex, the components and preparation method are as follows:

[0076] 15 parts bentonite, 9 parts maltodextrin, 9 parts microbial inoculant, 6 parts polyacrylamide, 15 parts oat bran, 6 parts cow dung, and 9 parts brown algae.

[0077] The ratio of viable counts of nitrifying bacteria, Rhizobium radiophyllum, Bacillus licheniformis, and Bacillus megaterium in the microbial agent is 1:2:1:0.8, with a total viable count of 3.0 × 10⁻⁶. 9 cfu / g.

[0078] The preparation method is the same as in Example 1.

[0079] Test Example 1

[0080] 1. Experimental Methods

[0081] A field comparison trial was conducted with eight treatments: control group (CK), Example 1 group (A), Example 2 group (B), Comparative Example 1 group (C), Comparative Example 2 group (D), Comparative Example 3 group (E), Comparative Example 4 group (F), and Comparative Example 5 group (G). Each treatment was replicated in triplicate using a randomized block design, with each plot measuring 16m. 2 .

[0082] Rice was sown around the Qingming Festival, using dry-seedling cultivation with insulation, and transplanted in early May. At transplanting, the organic compound from groups AG (Examples 1, 2, Comparative Examples 1, 2, 3, 4, and 5) was applied in a single application according to the design requirements, without further topdressing, at a rate of 3.0 t / hm². 2 The control group did not receive any organic compounds. Field management, weeding, and pest and disease control were carried out according to local practices, and were completely consistent across all treatments.

[0083] 2. Measurement Indicators

[0084] Harvesting takes place in early October, and yield is calculated based on actual output. Plant samples are taken at harvest time to measure relevant biological indicators.

[0085] 3. Data Processing

[0086] Data processing was performed using Graphpad Prism 7.0 software, and the measurement data was presented in the following format: The comparison between groups was performed using SNK-q, with P < 0.05 considered statistically significant.

[0087] 4. Measurement Results

[0088] As shown in Table 1, compared with the control group (CK) without the application of organic compound, the organic compound treatments in groups A and B increased the number of rice panicles. The effects of groups A and B in increasing the number of rice panicles were the most significant. The effects of groups A and B in increasing the number of rice panicles were significantly better than those in groups C, D, E, F, and G.

[0089] Table 1. Number of spikelets under different treatments

[0090] Group <![CDATA[Number of productive ears (10,000 / hm 2 )]]> CK group 181.8±12.9a Group A 292.4±9.0b Group B 288.8±14.6b Group C 231.2±9.3c Group D 240.0±14.3c Group E 233.1±16.9c Group F 225.7±24.8c Group G 223.5±14.1c

[0091] Note: Different lowercase letters indicate significant differences between different groups (P < 0.05).

[0092] As shown in Table 2, compared with the control group (CK) without the application of organic compound, the organic compound treatments in groups A and B increased the number of filled grains per panicle in rice. The effects of groups A and B in increasing the number of filled grains per panicle were the most significant. The effects of groups A and B in increasing the number of filled grains per panicle were significantly better than those in groups C, D, E, F, and G.

[0093] Table 2 Number of filled grains per ear under different treatments

[0094] Group Number of grains per ear CK group 78.8±9.5a Group A 112.5±10.5b Group B 114.3±13.0b Group C 91.5±6.8c Group D 94.1±8.4c Group E 95.6±12.0c Group F 93.9±5.8c Group G 91.4±10.3c

[0095] Note: Different lowercase letters indicate significant differences between different groups (P < 0.05).

[0096] As shown in Table 3, compared with the CK group (no organic compound treatment), the organic compound treatments in groups A and B increased the dry grain weight of rice. Groups A and B showed the most significant effect in increasing the dry grain weight of rice. The effect of groups A and B in increasing the dry grain weight of rice was significantly better than that of groups C, D, E, F, and G.

[0097] Table 3 Dry Particle Weight under Different Treatments

[0098] Group Dry grain weight (g) CK group 26.1±3.0a Group A 34.7±2.0b Group B 33.0±1.5b Group C 28.3±0.8a Group D 27.4±2.4a Group E 27.9±1.4a Group F 28.3±1.1a Group G 28.3±0.9a

[0099] Note: Different lowercase letters indicate significant differences between different groups (P < 0.05).

[0100] like Figure 1 As shown, compared with the control group (CK) without the application of organic compounds, the organic compound treatments in groups A and B increased rice yield. Groups A and B showed the most significant yield-increasing effects. The yield-increasing effects of groups A and B were significantly better than those of groups C, D, E, F, and G.

[0101] The organic compound of this invention increases the number of rice panicles, the number of filled grains per panicle, and the dry grain weight of rice, effectively improving rice yield.

[0102] Test Example 2

[0103] 1. Experimental Methods

[0104] A field comparison trial was conducted with five treatments: control group (CK), Example 1 group (A), Example 3 group (B), Comparative Example 1 group (C), and Comparative Example 2 group (D). Each treatment was replicated in triplicate using a randomized block design, with each plot measuring 25m. 2 .

[0105] The tested variety was potato, planted manually at a depth of 15 cm. The organic compound of this invention was applied at the initial flowering stage of the potatoes. The organic compound from groups AD (Example 1, Example 2, Comparative Example 1, and Comparative Example 2) was applied all at once, without further topdressing, at a rate of 3.5 t / hm². 2 The control group did not receive any organic compounds. Field management, weeding, and pest and disease control were carried out according to local practices, and were completely consistent across all treatments.

[0106] 2. Measurement Indicators

[0107] After harvest, the yield is calculated based on the actual output. At harvest time, plant samples are taken to measure the yield of tubers in each plot and the equivalent yield.

[0108] 3. Data Processing

[0109] Data processing was performed using Graphpad Prism 7.0 software, and the measurement data was presented in [the format of the original text]. The comparison between groups was performed using SNK-q, with P < 0.05 considered statistically significant.

[0110] 4. Measurement Results

[0111] like Figure 2 As shown, compared with the control group (CK) without the application of organic compounds, the organic compound treatments in groups A and B increased the yield of tubers in the plots. The effects of groups A and B in increasing tuber yield were the most significant. The effects of groups A and B in increasing tuber yield were significantly better than those in groups C and D.

[0112] like Figure 3 As shown, compared with the control group (CK) without the application of the organic compound, the organic compound treatments in groups A and B increased the equivalent yield. The effects of increasing equivalent yield were most significant in groups A and B. The effects of increasing equivalent yield in groups A and B were significantly better than those in groups C and D.

[0113] After the formulation of the organic compound was changed in Comparative Examples 1-5, the effect of improving soil fertility became worse. The applicant believes that the components of the organic compound of the present invention have a synergistic effect, thereby achieving the technical effect of significantly improving soil fertility.

[0114] The present invention has been described in detail above with general descriptions and specific embodiments. However, modifications or improvements can be made to the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. An organic complex, characterized in that, The organic complex is made from the following raw materials in parts by weight: 10-20 parts humic acid, 6-12 parts maltodextrin, 6-12 parts microbial inoculant, 3-8 parts polyacrylamide, 10-20 parts oat bran, 3-8 parts cow dung, and 6-12 parts brown algae.

2. The organic complex according to claim 1, characterized in that, The organic complex is made from the following raw materials in parts by weight: 15 parts humic acid, 9 parts maltodextrin, 9 parts microbial inoculant, 6 parts polyacrylamide, 15 parts oat bran, 6 parts cow dung, and 9 parts brown algae.

3. The organic complex according to claim 1, characterized in that, The organic complex is made from the following raw materials in parts by weight: 20 parts humic acid, 6 parts maltodextrin, 12 parts microbial inoculant, 3 parts polyacrylamide, 10 parts oat bran, 3 parts cow dung, and 12 parts brown algae.

4. The organic complex according to claim 1, characterized in that, The organic complex is made from the following raw materials in parts by weight: 10 parts humic acid, 12 parts maltodextrin, 6 parts microbial inoculant, 8 parts polyacrylamide, 20 parts oat bran, 8 parts cow dung, and 6 parts brown algae.

5. The organic complex according to any one of claims 1-4, characterized in that, The microbial agent includes nitrifying bacteria, rhizobium radiation, Bacillus licheniformis, and Bacillus megaterium with a live bacteria ratio of 1:1.0-2.5:0.8-1.5:0.5-1.

3.

6. The organic complex according to claim 5, characterized in that, The ratio of viable counts of nitrifying bacteria, rhizobium radiation, Bacillus licheniformis, and Bacillus megaterium is 1:2:1:0.

8.

7. The organic complex according to any one of claims 1-4, characterized in that, The preparation method of the organic compound is as follows: (1) air-dry cow dung and crush it; (2) mix microbial inoculants evenly; (3) mix humic acid, maltodextrin, polyacrylamide, oat bran and brown algae, and then mix with cow dung and microbial inoculants to obtain the organic compound of the present invention.

8. Use of the organic compound according to any one of claims 1-4 in improving soil fertility.

9. The use according to claim 8, characterized in that, The organic compound increases the number of panicles and the dry grain weight of rice.

10. The use according to claim 8, characterized in that, The organic compound increases the number of grains per panicle of rice.

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