Magnaporthe oryzae inoculation device and method

Abstract

The invention discloses a magnaporthe oryzae inoculation device and method, and relates to the technical field of plant pathology research instruments.The magnaporthe oryzae inoculation device comprises an inoculation box, the top of the inoculation box is fixedly connected with a water tank used for keeping the humidity of the humid environment in the inoculation box, and two inoculation assemblies used for inoculation are arranged outside the inoculation box; the bottom of the inoculation box is fixedly connected with a control box; and an observation window for observing the growth condition of rice after internal inoculation is arranged on the outer wall of the inoculation box. Through the opposite-direction rotation system of the flow guide plate and the culture tank, the inclined plane design of the flow guide plate and the inclined plane design of the circular baffle and the flow guide plate, uniform coverage of rice leaves by liquid medicine, improvement of inoculation efficiency and liquid medicine utilization rate, integration of light supplement, humidity control and waste liquid separation functions, optimization of experimental conditions and reduction of operation complexity are realized; the growth condition of the rice can be recorded without opening the device through the observation window and the transparent circular baffle, and environmental interference is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant pathology research apparatus, and particularly relates to a Magnaporthe oryzae inoculation device and method. BACKGROUND

[0002] Magnaporthe oryzae is a major disease of rice, which has the characteristics of wide range of disease, strong pathogenicity and serious harm, and can cause rice yield reduction, and even absolute yield reduction in severe cases, and is one of the key factors restricting global rice production safety. In order to effectively prevent and control rice blast, a large amount of basic research (such as pathogenicity analysis of the pathogen and analysis of the disease resistance mechanism of rice) and applied research (such as disease-resistant variety screening and fungicide effect evaluation) need to be carried out in scientific research and production, and pathogen inoculation is the core link of these researches. Through artificial inoculation of Magnaporthe oryzae on rice plants, the natural disease process is simulated, and the disease condition is observed to obtain experimental data.

[0003] According to the Chinese patent document CN209098680U, a Magnaporthe oryzae inoculation device is disclosed, which comprises an inoculation box, a plurality of fixed platforms are arranged in the inoculation box from bottom to top, an inoculation turntable is connected to the fixed platform through a motor, and an inoculation dish is placed on the inoculation turntable. A water storage tank is arranged on one side of the inoculation box, a first water pump is connected to the water storage tank through a first connecting pipe, the first water pump is communicated with the inoculation box through a first water outlet pipe, and a first atomizing nozzle is connected to the first water outlet pipe corresponding to each inoculation turntable. A inoculation liquid storage tank is arranged on one side of the inoculation box, a second water pump is connected to the inoculation liquid storage tank through a second connecting pipe, the second water pump is communicated with the inoculation box through a second water outlet pipe, and a second atomizing nozzle is connected to the second water outlet pipe corresponding to each inoculation turntable. The rice pathogen inoculation can be automatically and uniformly carried out, and the water can be automatically and uniformly sprayed, thereby saving manpower and improving work efficiency.

[0004] The above-mentioned device can not control the process of spraying the liquid in the inoculation liquid storage tank through the atomizing nozzle and letting the spray itself fall on the rice leaves to be inoculated, which may cause waste of liquid and unsatisfactory inoculation effect. No waste liquid discharge structure is provided, which may pollute the internal environment of the inoculation box. SUMMARY

[0005] The present application adopts a conical boss design for the flow guide plate, the surface inclination angle is parallel to the inoculation nozzle, ensures that the liquid medicine sprayed by the inoculation nozzle falls on the surface of the flow guide plate, then the liquid medicine uniformly slides along the inclined surface to the rice leaves, the motor drives the culture tank to rotate, makes all the rice plants uniformly contact the liquid medicine, the culture tank and the flow guide plate rotate reversely through the gear system, uses the centrifugal force to throw the liquid medicine to the edge, covers a wider area, improves the utilization rate of the liquid medicine, reduces the waste of the liquid medicine, the circular baffle is made of transparent corrosion-resistant material, can avoid the liquid medicine flowing to other positions, through the side wall observation window, the growth and disease of the rice can be directly recorded, without opening the box, the flow guide plate is provided with a light supplement lamp, provides stable light conditions, ensures the normal growth of the rice, the water tank and the atomizing nozzle keep the humid environment in the inoculation box, provides conditions for the growth of the rice blast fungus.

[0006] To solve the above technical problems, the technical scheme adopted by the present application is: A rice blast fungus inoculation device and method, comprising an inoculation box, the top of the inoculation box is fixedly connected with a water tank for keeping the internal humid environment of the inoculation box, the outside of the inoculation box is provided with two groups of inoculation assemblies for inoculation, the bottom of the inoculation box is fixedly connected with a control box, and the outer wall of the inoculation box is provided with an observation window for observing the growth of the rice after inoculation.

[0007] Preferably, the inner cavity of the inoculation box is fixedly connected with a liquid leakage structure near the bottom, the center of the liquid leakage structure is fixedly connected with a motor, the surface of the liquid leakage structure is rotatably connected with a culture tank for planting rice, the output end of the motor is fixedly connected with the culture tank, the upper surface of the culture tank is fixedly connected with a circular baffle for blocking the inoculation liquid, the inside of the circular baffle is provided with a flow guide plate for gathering and dripping the liquid medicine, the bottom of the water tank is fixedly connected with a water pipe, the water pipe penetrates into the inside of the inoculation box and is fixedly connected with an atomizing nozzle, and there is a gap between the flow guide plate and the circular baffle for the liquid medicine to drip.

[0008] Preferably, the liquid leakage structure is a cylinder at the upper part and a gradually inclined downward cone at the lower part, the bottom center of the cone is fixedly connected with a cylindrical connecting column, the surface of the cylindrical part of the liquid leakage structure is fixedly connected with two mutually intersecting mounting plates, the connecting column is fixedly connected to the center of the inner wall of the bottom of the inoculation box, the flow guide plate is in the shape of a conical boss and has a cavity inside, the flow guide plate is made of transparent material, and the circular baffle is made of transparent corrosion-resistant material, so that the growth of the rice in the culture tank can be observed through the observation window.

[0009] Preferably, the inoculation assembly includes an inoculation pipe, a water pump is fixedly connected to the outer wall of the control box, two inoculation solution storage tanks for storing inoculation stock solution are provided inside the control box, one end of the inoculation pipe extends into the interior of the inoculation tank and is inclined, two inoculation nozzles for spraying inoculation stock solution are fixedly connected to the inclined portion of the inoculation pipe, one end of the inoculation pipe is fixedly connected to the outlet of the water pump, and the inlet of the water pump extends through a pipe into the interior of the control box and is fixedly connected to the inoculation solution storage tank.

[0010] Preferably, a leakage port is provided at the center of the bottom connecting column of the leakage structure, the leakage port extends into the interior of the control box, and a waste liquid tank is provided inside the control box near the lower part of the leakage port.

[0011] Preferably, the inclination angle of the inoculation pipe is parallel to the conical surface of the guide plate.

[0012] Preferably, a rotating shaft is fixedly connected to the center of the conical protrusion of the guide plate, and a plurality of supplementary lighting tubes for providing light to the rice are arranged inside the guide plate. An eccentric shaft is fixedly connected to the end of the rotating shaft away from the guide plate, and a cylindrical gear is fixedly connected to the end of the eccentric shaft away from the rotating shaft. An internal gear is fixedly connected to the inside of the cultivation tank near the rotating shaft. The internal gear meshes with the cylindrical gear. The internal gear is concentric with the cultivation tank. The eccentric shaft and the cylindrical gear are rotatably connected to the cultivation tank.

[0013] Due to the adoption of the above technical solution, the technical progress achieved by this invention compared to the prior art is as follows: 1. This invention uses a conical boss design for the guide plate, with the surface tilt angle parallel to the inoculation nozzle, to ensure that the liquid medicine slides evenly onto the rice leaves along the inclined surface. The motor drives the culture tank to rotate, so that all rice plants are evenly in contact with the liquid medicine. The guide plate and the culture tank rotate in opposite directions through a gear system, using centrifugal force to throw the liquid medicine to the edge, covering a wider area. The leakage structure separates the waste liquid from the plants, preventing pathogen residue.

[0014] 2. This invention uses a corrosion-resistant material for the transparent circular baffle, combined with a side observation window, which allows for direct recording of rice growth and disease incidence without opening the box. The baffle integrates supplemental lighting tubes to provide stable lighting conditions and ensure normal rice growth. The water tank and atomizing nozzle maintain a humid environment inside the inoculation box, providing conditions for the growth of rice blast pathogens. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the inoculation box of the present invention; Figure 3This is a schematic diagram of the leakage structure of the present invention; Figure 4 This is a schematic diagram of the drug solution guiding structure of the present invention; Figure 5 For the present invention Figure 4 Enlarged structural diagram at point A in the middle.

[0016] In the diagram: 1. Inoculation box; 11. Guide plate; 111. Supplemental light tube; 112. Rotating shaft; 1121. Eccentric shaft; 1122. Cylindrical gear; 12. Circular baffle; 13. Culture tank; 131. Internal gear; 14. Leakage structure; 141. Leakage port; 142. Waste liquid tank; 15. Motor; 2. Inoculation assembly; 21. Inoculation pipeline; 22. Inoculation nozzle; 23. Water pump; 24. Inoculation solution storage tank; 3. Control box; 4. Water tank; 41. Atomizing nozzle; 5. Observation window. Detailed Implementation

[0017] To make the technical means, creative features, objectives, and effects of this invention easier to understand, the invention is further described below in conjunction with specific embodiments: like Figure 1 As shown, this invention provides a rice blast fungus inoculation device, including an inoculation box 1. A water tank 4, used to maintain a humid environment inside the inoculation box 1, is fixedly connected to the top of the box. Two sets of inoculation components 2 are installed on the outside of the box 1. A control box 3 is fixedly connected to the bottom of the box 1. An observation window 5 is provided on the outer wall of the box 1 for observing the growth of rice after inoculation. The inoculation box 1 and control box 3 are separated to avoid contamination of the internal environment of the box 1, which could affect the experimental results. The observation window 5 allows for recording the daily growth of rice without opening the box 1, while also reducing the frequency of cross-contamination between the internal and external environments, thus preventing contamination of the internal environment. The inoculation components 2 spray the stock solution containing rice blast fungus onto the surface of the rice leaves, and the water tank 4 sprays water into the box 1 to maintain a humid environment.

[0018] like Figure 2As shown, a liquid leakage structure 14 is fixedly connected to the bottom of the inner cavity of the inoculation box 1. A motor 15 is fixedly connected to the center of the liquid leakage structure 14. A rice cultivation trough 13 is rotatably connected to the surface of the liquid leakage structure 14. The output end of the motor 15 is fixedly connected to the cultivation trough 13. A circular baffle 12 is fixedly connected to the upper surface of the cultivation trough 13 to block the inoculation liquid. A guide plate 11 is provided inside the circular baffle 12 to collect and drip the liquid. A water pipe is fixedly connected to the bottom of the water tank 4 and extends through the inside of the inoculation box 1. An atomizing nozzle 41 is fixedly connected to the water pipe. There is a gap between the guide plate 11 and the circular baffle 12 for the liquid to drip. Rice plants in the cultivation tank 13 are planted in a circular pattern below the gaps. The inoculation component 2 sprays the original liquid containing rice blast fungicide onto the surface of the guide plate 11. The liquid slides down the conical surface of the guide plate 11 and finally drips onto the surface of the rice leaves. The liquid gradually flows from the tip of the leaf to the root of the leaf, ensuring that the leaf is in full contact with the liquid. The motor 15 rotates the rice plants in the cultivation tank 13 to ensure that each rice plant is in full contact with the liquid. The atomizing nozzle 41 sprays water from the water tank 4 into the inoculation box 1 in the form of water mist, keeping the internal environment of the inoculation box 1 humid. Excess water mist forms water droplets on the inner wall of the inoculation box 1 and finally drips into the leakage structure 14. At the same time, the water mist can also absorb and carry away the floating dust in the inoculation box 1.

[0019] A filter screen is installed at the bottom of the culture tank 13 to filter out excess rice blast pathogens in the culture tank 13 and drain them into the leakage structure 14.

[0020] The leakage structure 14 is cylindrical at the top and gradually sloping downwards into a cone shape. A cylindrical connecting column is fixedly connected to the center of the bottom of the cone. Two intersecting mounting plates are fixedly connected to the surface of the cylindrical part of the leakage structure 14. The connecting column is fixedly connected to the center of the bottom inner wall of the inoculation box 1. The guide plate 11 is a conical boss shape with an internal cavity. The guide plate 11 is made of transparent material. The circular baffle 12 is made of transparent and corrosion-resistant material, facilitating observation of the growth of rice in the cultivation tank 13 through the observation window 5. The conical structure improves the flow of waste liquid and prevents it from accumulating in one place. The transparent circular baffle 12 allows researchers to easily observe the growth of rice after inoculation with rice blast fungus through the observation window. The corrosion resistance extends the service life of the device.

[0021] like Figure 3As shown, the inoculation assembly 2 includes an inoculation pipe 21. A water pump 23 is fixedly connected to the outer wall of the control box 3. The control box 3 contains two inoculation solution storage tanks 24 for storing the inoculation stock solution. One end of the inoculation pipe 21 extends into the interior of the inoculation box 1 and is inclined. Two inoculation nozzles 22 for spraying the inoculation stock solution are fixedly connected to the inclined portion of the inoculation pipe 21. One end of the inoculation pipe 21 is fixedly connected to the outlet of the water pump 23. The inlet of the water pump 23 extends through a pipe into the interior of the control box 3 and is fixedly connected to the inoculation solution storage tanks 24. The inoculation stock solution is a mixed solution containing rice blast fungus spores. The water pump 23 draws the solution from the inoculation solution storage tanks 24, passes it through the inoculation pipe 21, and finally sprays it out from the inoculation nozzles 22. The sprayed solution adheres to the surface of the guide plate 11 and flows towards the surface of the rice leaves.

[0022] A leakage port 141 is provided at the center of the bottom connecting column of the leakage structure 14, and the leakage port 141 extends into the interior of the control box 3. A waste liquid tank 142 is provided inside the control box 3 near the lower part of the leakage port 141. The waste liquid collected in the leakage structure 14 flows through the conical structure to the leakage port 141 and is discharged into the waste liquid tank 142, where it is finally disposed of in a unified manner.

[0023] The inclination angle of the inoculation pipe 21 is parallel to the conical surface of the guide plate 11. When parallel, it can improve the effect of the medicine sprayed from the inoculation nozzle 22 on the surface of the guide plate 11, and reduce the waste of medicine.

[0024] like Figure 4 and Figure 5As shown, a rotating shaft 112 is fixedly connected to the center of the conical protrusion of the guide plate 11. Several supplementary lighting tubes 111 are provided inside the guide plate 11 to provide light for the rice. An eccentric shaft 1121 is fixedly connected to the end of the rotating shaft 112 away from the guide plate 11. A cylindrical gear 1122 is fixedly connected to the end of the eccentric shaft 1121 away from the rotating shaft 112. An internal gear 131 is fixedly connected to the inside of the cultivation tank 13 near the rotating shaft 112. The internal gear 131 meshes with the cylindrical gear 1122. The internal gear 131 is concentric with the cultivation tank 13. The eccentric shaft 1121 and the cylindrical gear 1122 are rotatably connected to the cultivation tank 13. The supplemental light tube 111 and the motor 15 are electrically connected via an external power supply. Through the eccentric shaft 1121 and the cylindrical gear 1122, when the cultivation tank 13 rotates with the meshing gear 131, the gear meshing drives the cylindrical gear 1122 and the eccentric shaft 1121 to rotate, which in turn drives the rotating shaft 112 and the guide plate 11 to rotate. The guide plate 11 and the cultivation tank 13 rotate in opposite directions under the action of the gear. When the liquid medicine on the surface flows to the edge through the rotation of the guide plate 11, the centrifugal force can cause the water droplets formed by the liquid medicine to be thrown out. The thrown liquid medicine can be blocked by the circular baffle 12. By changing the rotation speed of the guide plate 11, the centrifugal force can be changed, so that the liquid medicine can fill the gap between the guide plate 11 and the circular baffle 12, thereby improving the inoculation efficiency of rice in the gap.

[0025] The working principle of this invention is as follows: Rice is placed on the cultivation tank 13 in the gap between the guide plate 11 and the circular baffle 12. The rice blast fungus stock solution is sprayed onto the surface of the guide plate 11 through the inoculation nozzle 22. Through the action of the motor and gears, the cultivation tank 13 and the guide plate 11 are rotated in opposite directions, and the medicine solution on the surface of the guide plate 11 is dripped onto the surface of the rice leaves. Water is sprayed into the inoculation box 1 through the water tank 4 and the atomizing nozzle 41 to keep the internal environment of the inoculation box 1 moist and promote the growth of rice blast fungus. The supplemental light tube 111 can provide stable light conditions to ensure normal rice growth. The growth of rice is observed through the observation window 5.

[0026] The present invention has been described in detail above. However, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, any modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A rice blast fungus inoculation device, comprising an inoculation box (1), characterized in that: The top of the inoculation box (1) is fixedly connected to a water tank (4) to maintain a humid environment inside the inoculation box (1). Two sets of inoculation components (2) for inoculation are provided on the outside of the inoculation box (1). A control box (3) is fixedly connected to the bottom of the inoculation box (1). An observation window (5) for observing the growth of rice after inoculation is provided on the outer wall of the inoculation box (1).

2. The rice blast fungus inoculation device according to claim 1, characterized in that: The inoculation box (1) has a leaking structure (14) fixedly connected to the bottom of its inner cavity. A motor (15) is fixedly connected to the center of the leaking structure (14). A rice cultivation trough (13) is rotatably connected to the surface of the leaking structure (14). The output end of the motor (15) is fixedly connected to the cultivation trough (13). A circular baffle (12) is fixedly connected to the upper surface of the cultivation trough (13) to block the inoculation solution. A guide plate (11) for collecting and dripping the solution is provided inside the circular baffle (12). A water pipe is fixedly connected to the bottom of the water tank (4) and extends through the inside of the inoculation box (1) to a fixedly connected atomizing nozzle (41). There is a gap between the guide plate (11) and the circular baffle (12) for the solution to drip.

3. The rice blast fungus inoculation device according to claim 2, characterized in that: The leakage structure (14) is a cylinder at the top and a cone that gradually slopes downwards at the bottom. A cylindrical connecting column is fixedly connected to the center of the bottom of the cone. Two intersecting mounting plates are fixedly connected to the surface of the cylindrical part of the leakage structure (14). The connecting column is fixedly connected to the center of the bottom inner wall of the inoculation box (1). The guide plate (11) is a cone-shaped boss with an internal cavity. The guide plate (11) is made of transparent material. The circular baffle (12) is made of transparent and corrosion-resistant material, which facilitates observation of the growth of rice on the cultivation tank (13) through the observation window (5).

4. The rice blast fungus inoculation device according to claim 3, characterized in that: The inoculation assembly (2) includes an inoculation pipe (21). A water pump (23) is fixedly connected to the outer wall of the control box (3). The control box (3) is equipped with two inoculation solution storage tanks (24) for storing inoculation stock solution. One end of the inoculation pipe (21) extends into the inoculation tank (1) and is inclined. The inclined part of the inoculation pipe (21) is fixedly connected to two inoculation nozzles (22) for spraying out inoculation stock solution. One end of the inoculation pipe (21) is fixedly connected to the outlet of the water pump (23). The inlet of the water pump (23) extends through the pipe into the control box (3) and is fixedly connected to the inoculation solution storage tank (24).

5. The rice blast fungus inoculation device according to claim 3, characterized in that: The bottom connecting column of the leakage structure (14) has a leakage port (141) at the center, and the leakage port (141) extends into the interior of the control box (3). The interior of the control box (3) is provided with a waste liquid tank (142) near the leakage port (141).

6. The rice blast fungus inoculation device according to claim 4, characterized in that: The inclination angle of the inoculation pipe (21) is parallel to the conical surface of the guide plate (11).

7. The rice blast fungus inoculation device according to claim 2, characterized in that: A rotating shaft (112) is fixedly connected to the center of the conical protrusion of the guide plate (11). Several supplementary lighting tubes (111) for providing light to rice are arranged inside the guide plate (11). An eccentric shaft (1121) is fixedly connected to the end of the rotating shaft (112) away from the guide plate (11). A cylindrical gear (1122) is fixedly connected to the end of the eccentric shaft (1121) away from the rotating shaft (112). An internal gear (131) is fixedly connected to the inside of the cultivation tank (13) near the rotating shaft (112). The internal gear (131) and the cylindrical gear (1122) mesh with each other. The internal gear (131) is concentric with the cultivation tank (13). The eccentric shaft (1121) and the cylindrical gear (1122) are rotatably connected to the cultivation tank (13).

8. A method for inoculating rice blast fungus, comprising the rice blast fungus inoculation device according to claims 1-7, characterized in that, The specific steps are as follows: Step 1: First, plant rice in the gap between the guide plate (11) and the circular baffle (12) in the cultivation trough (13); Step 2: Start the water pump (23) and motor (15) to spray the liquid medicine onto the surface of the guide plate (11). The motor (15) drives the guide plate (11) and the culture tank (13) to rotate in opposite directions, so that the liquid medicine on the surface of the guide plate (11) drips onto the surface of the rice leaves. Step 3: After the medicine is sprayed, the atomizing nozzle (41) sprays water in the form of water mist into the inside of the inoculation box (1) to keep the inside environment of the inoculation box (1) moist. Step 4: Observe the growth of rice through the reserved observation window (5) and the transparent prototype baffle (12), and record the experimental results.

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